Carg13500 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13500
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein-serine/threonine phosphatase
Location	Carg_Chr08: 3150001 .. 3150699 (+)
RNA-Seq Expression	Carg13500
Synteny	Carg13500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCTGTTTGTTTGCCAAGAATTTGTGGAGGCTTCATTTTTCGGTCGCTGTTAGTTTTGATGTTGATTTTGACTTGATTTGGAGGGAGTTTACGAAGTATGTATGGATGTATGGATGTATGTTGTATGATGTATTTTCATTTGTTTGAATCTTGTGTCTGCTCTGAAACCTAAACGACAGTTTGGAAGATGGAGGGATGGTGTTGTGAGAAATGAACATTTCGATGTGTTTTAGTTGGAAGTTCGGAACCTATTGGTTTTTGTATTATTTTTCTTGAGAAAATGCATTGGGACGGACGGATACCGTTTTACTCCGATTAGTCTCTGACAATTAGATTGATCCTTTCAGTTTTTCCCCCTTCCCCTTGGAAATGATGTGAACTCTGCCTACAAATGATTTTTCTGTGAGGGGGTGACCTTTTGGTGATTCATGGTTTTTCTCTAATTCCAGATTGAAAATGGGGATATATCTGAGCTCCCCCAAAACAGAAAAGGCCTCGGATGATGGTGAAAACGAAAGACTTCGGTACGGCCTTTCCTCAATGCAAGGGTGGCGTGCAACCATGGAAGATGCTGTGAGTTTTCCTTCTACTGTATCCGTTCCCTTTGACGTTGAAGTCTGTGCCATGTTATTGTACTCAGTTGTTCATCATGCACTCTATTGCATACTGCCATCCATTCAAATTAATATATCATAG

mRNA sequence

ATGATCTGTTTGTTTGCCAAGAATTTGTGGAGGCTTCATTTTTCGGTCGCTGTTAGTTTTGATGTTGATTTTGACTTGATTTGGAGGGAGTTTACGAAATTGAAAATGGGGATATATCTGAGCTCCCCCAAAACAGAAAAGGCCTCGGATGATGGTGAAAACGAAAGACTTCGGTACGGCCTTTCCTCAATGCAAGGGTGGCGTGCAACCATGGAAGATGCTGTGAGTTTTCCTTCTACTGTATCCGTTCCCTTTGACGTTGAAGTCTGTGCCATGTTATTGTACTCAGTTGTTCATCATGCACTCTATTGCATACTGCCATCCATTCAAATTAATATATCATAG

Coding sequence (CDS)

ATGATCTGTTTGTTTGCCAAGAATTTGTGGAGGCTTCATTTTTCGGTCGCTGTTAGTTTTGATGTTGATTTTGACTTGATTTGGAGGGAGTTTACGAAATTGAAAATGGGGATATATCTGAGCTCCCCCAAAACAGAAAAGGCCTCGGATGATGGTGAAAACGAAAGACTTCGGTACGGCCTTTCCTCAATGCAAGGGTGGCGTGCAACCATGGAAGATGCTGTGAGTTTTCCTTCTACTGTATCCGTTCCCTTTGACGTTGAAGTCTGTGCCATGTTATTGTACTCAGTTGTTCATCATGCACTCTATTGCATACTGCCATCCATTCAAATTAATATATCATAG

Protein sequence

MICLFAKNLWRLHFSVAVSFDVDFDLIWREFTKLKMGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVSFPSTVSVPFDVEVCAMLLYSVVHHALYCILPSIQINIS

Homology

[Close]

BLAST of Carg13500 vs. NCBI nr
Match: KAG7025709.1 (putative protein phosphatase 2C 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 232.6 bits (592), Expect = 1.6e-57
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MICLFAKNLWRLHFSVAVSFDVDFDLIWREFTKLKMGIYLSSPKTEKASDDGENERLRYG 60
           MICLFAKNLWRLHFSVAVSFDVDFDLIWREFTKLKMGIYLSSPKTEKASDDGENERLRYG
Sbjct: 1   MICLFAKNLWRLHFSVAVSFDVDFDLIWREFTKLKMGIYLSSPKTEKASDDGENERLRYG 60

Query: 61  LSSMQGWRATMEDAVSFPSTVSVPFDVEVCAMLLYSVVHHALYCILPSIQINIS 115
           LSSMQGWRATMEDAVSFPSTVSVPFDVEVCAMLLYSVVHHALYCILPSIQINIS
Sbjct: 61  LSSMQGWRATMEDAVSFPSTVSVPFDVEVCAMLLYSVVHHALYCILPSIQINIS 114

[Close]

BLAST of Carg13500 vs. NCBI nr
Match: KAG7014188.1 (putative protein phosphatase 2C 60, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 97.4 bits (241), Expect = 8.2e-17
Identity = 47/54 (87.04%), Postives = 49/54 (90.74%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVSFPSTVSVPFDVEV 90
          MGIYLSSPKTEKASDDGENE LRYG+SSMQGWRATMEDAVSF S VS PFDV +
Sbjct: 1  MGIYLSSPKTEKASDDGENESLRYGVSSMQGWRATMEDAVSFLSIVSAPFDVNL 54

[Close]

BLAST of Carg13500 vs. NCBI nr
Match: KAG6593363.1 (putative protein phosphatase 2C 60, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 89.4 bits (220), Expect = 2.2e-14
Identity = 46/63 (73.02%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 12 LHFSVAVSFDVDFDLIWREFTKLKMGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATM 71
          LHFS  +S       ++     LKMGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATM
Sbjct: 21 LHFSRKIS-------LFEALGGLKMGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATM 76

Query: 72 EDA 75
          EDA
Sbjct: 81 EDA 76

[Close]

BLAST of Carg13500 vs. NCBI nr
Match: KAF5741796.1 (protein phosphatase 2C 21 isoform X1 [Tripterygium wilfordii])

HSP 1 Score: 85.1 bits (209), Expect = 4.2e-13
Identity = 43/64 (67.19%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 28 WREFTKLK--MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA----VSFPSTV 86
          WR  T+ +  MG+YLSSPKTEKAS+DGENERLRYGLSSMQGWRATMEDA    + F S+ 
Sbjct: 18 WRFLTRFENIMGVYLSSPKTEKASEDGENERLRYGLSSMQGWRATMEDAHQAYLDFDSST 77

[Close]

BLAST of Carg13500 vs. NCBI nr
Match: KAB5564924.1 (hypothetical protein DKX38_004978 [Salix brachista])

HSP 1 Score: 84.3 bits (207), Expect = 7.2e-13
Identity = 47/79 (59.49%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 36  MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVSFPSTVSVPFDVEVCA--ML 95
           MG+YLSSPKT+KAS DGEN+RLRYGLSSMQGWRATMEDAVS      V F    C   ML
Sbjct: 1   MGVYLSSPKTDKASQDGENDRLRYGLSSMQGWRATMEDAVSTLYDFCVAFVKIQCLGYML 60

Query: 96  LYSVVHHALYCILPSIQIN 113
           +  V+H  +   + S  IN
Sbjct: 61  MLKVIHFGIVIAVWSRGIN 79

[Close]

BLAST of Carg13500 vs. ExPASy Swiss-Prot
Match: O81716 (Probable protein phosphatase 2C 21 OS=Arabidopsis thaliana OX=3702 GN=PPC4-2 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 9.8e-13
Identity = 33/39 (84.62%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MG YLSSPKTEK S+DGEN++LR+GLSSMQGWRATMEDA
Sbjct: 1  MGTYLSSPKTEKLSEDGENDKLRFGLSSMQGWRATMEDA 39

[Close]

BLAST of Carg13500 vs. ExPASy Swiss-Prot
Match: Q67UP9 (Probable protein phosphatase 2C 58 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0651600 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-12
Identity = 32/41 (78.05%), Postives = 39/41 (95.12%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVS 77
          MG+YLS+PKTEK S+DGEN++L++GLSSMQGWRATMEDA S
Sbjct: 1  MGVYLSTPKTEKLSEDGENDKLKFGLSSMQGWRATMEDAHS 41

[Close]

BLAST of Carg13500 vs. ExPASy Swiss-Prot
Match: Q9SZ53 (Probable protein phosphatase 2C 60 OS=Arabidopsis thaliana OX=3702 GN=At4g31860 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-12
Identity = 32/39 (82.05%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MGIYLS+PKT+K S+DGEN +LRYGLSSMQGWRA+MEDA
Sbjct: 1  MGIYLSTPKTDKFSEDGENHKLRYGLSSMQGWRASMEDA 39

[Close]

BLAST of Carg13500 vs. ExPASy Swiss-Prot
Match: Q6ETK3 (Probable protein phosphatase 2C 11 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0180000 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 3.1e-11
Identity = 31/41 (75.61%), Postives = 37/41 (90.24%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVS 77
          MGIYLS+PKT+K S+DGEN++L+ GLSSMQGWRA MEDA S
Sbjct: 1  MGIYLSTPKTDKFSEDGENDKLKLGLSSMQGWRANMEDAHS 41

[Close]

BLAST of Carg13500 vs. ExPASy Swiss-Prot
Match: Q653S3 (Probable protein phosphatase 2C 70 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0558000 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 4.1e-11
Identity = 30/39 (76.92%), Postives = 35/39 (89.74%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MG+YLS+PKTEK S +G N+RLRYGL+SMQGWR TMEDA
Sbjct: 1  MGVYLSTPKTEKYSGEGGNDRLRYGLASMQGWRTTMEDA 39

[Close]

BLAST of Carg13500 vs. ExPASy TrEMBL
Match: A0A7J7D6U7 (Protein-serine/threonine phosphatase OS=Tripterygium wilfordii OX=458696 GN=HS088_TW10G00802 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.0e-13
Identity = 43/64 (67.19%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 28 WREFTKLK--MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA----VSFPSTV 86
          WR  T+ +  MG+YLSSPKTEKAS+DGENERLRYGLSSMQGWRATMEDA    + F S+ 
Sbjct: 18 WRFLTRFENIMGVYLSSPKTEKASEDGENERLRYGLSSMQGWRATMEDAHQAYLDFDSST 77

[Close]

BLAST of Carg13500 vs. ExPASy TrEMBL
Match: A0A5N5NBL1 (Protein-serine/threonine phosphatase OS=Salix brachista OX=2182728 GN=DKX38_004978 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 3.5e-13
Identity = 47/79 (59.49%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 36  MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDAVSFPSTVSVPFDVEVCA--ML 95
           MG+YLSSPKT+KAS DGEN+RLRYGLSSMQGWRATMEDAVS      V F    C   ML
Sbjct: 1   MGVYLSSPKTDKASQDGENDRLRYGLSSMQGWRATMEDAVSTLYDFCVAFVKIQCLGYML 60

Query: 96  LYSVVHHALYCILPSIQIN 113
           +  V+H  +   + S  IN
Sbjct: 61  MLKVIHFGIVIAVWSRGIN 79

[Close]

BLAST of Carg13500 vs. ExPASy TrEMBL
Match: A0A6J1KZ81 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111497691 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.0e-13
Identity = 39/39 (100.00%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA
Sbjct: 1  MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 39

[Close]

BLAST of Carg13500 vs. ExPASy TrEMBL
Match: A0A6J1HB24 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461180 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.0e-13
Identity = 39/39 (100.00%), Postives = 39/39 (100.00%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA
Sbjct: 1  MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 39

[Close]

BLAST of Carg13500 vs. ExPASy TrEMBL
Match: A0A6J1JMK1 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111488236 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.0e-12
Identity = 47/76 (61.84%), Postives = 54/76 (71.05%), Query Frame = 0


Query: 1  MICLFAKNLWRLHFSVAVSFDVDF--DLIWREFTKLKMGIYLSSPKTEKASDDGENERLR 60
          MI L AKN   +   + ++F+ +         FT  KMGIYLSSPKTEKASDDGENE LR
Sbjct: 1  MIRLLAKNSSVVVSLILINFEWELPRQACVASFT-WKMGIYLSSPKTEKASDDGENESLR 60

Query: 61 YGLSSMQGWRATMEDA 75
          YG+SSMQGWRATMEDA
Sbjct: 61 YGVSSMQGWRATMEDA 75

[Close]

BLAST of Carg13500 vs. TAIR 10
Match: AT2G25070.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 74.3 bits (181), Expect = 7.0e-14
Identity = 33/39 (84.62%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MG YLSSPKTEK S+DGEN++LR+GLSSMQGWRATMEDA
Sbjct: 1  MGTYLSSPKTEKLSEDGENDKLRFGLSSMQGWRATMEDA 39

[Close]

BLAST of Carg13500 vs. TAIR 10
Match: AT4G31860.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.0e-13
Identity = 32/39 (82.05%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MGIYLS+PKT+K S+DGEN +LRYGLSSMQGWRA+MEDA
Sbjct: 1  MGIYLSTPKTDKFSEDGENHKLRYGLSSMQGWRASMEDA 39

[Close]

BLAST of Carg13500 vs. TAIR 10
Match: AT4G31860.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.0e-13
Identity = 32/39 (82.05%), Postives = 37/39 (94.87%), Query Frame = 0


Query: 36 MGIYLSSPKTEKASDDGENERLRYGLSSMQGWRATMEDA 75
          MGIYLS+PKT+K S+DGEN +LRYGLSSMQGWRA+MEDA
Sbjct: 1  MGIYLSTPKTDKFSEDGENHKLRYGLSSMQGWRASMEDA 39

The following BLAST results are available for this feature:

BLAST of Carg13500 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025709.1	1.6e-57	100.00	putative protein phosphatase 2C 21, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG7014188.1	8.2e-17	87.04	putative protein phosphatase 2C 60, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6593363.1	2.2e-14	73.02	putative protein phosphatase 2C 60, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAF5741796.1	4.2e-13	67.19	protein phosphatase 2C 21 isoform X1 [Tripterygium wilfordii]	[more]
KAB5564924.1	7.2e-13	59.49	hypothetical protein DKX38_004978 [Salix brachista]	[more]

BLAST of Carg13500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81716	9.8e-13	84.62	Probable protein phosphatase 2C 21 OS=Arabidopsis thaliana OX=3702 GN=PPC4-2 PE=...	[more]
Q67UP9	1.3e-12	78.05	Probable protein phosphatase 2C 58 OS=Oryza sativa subsp. japonica OX=39947 GN=O...	[more]
Q9SZ53	2.8e-12	82.05	Probable protein phosphatase 2C 60 OS=Arabidopsis thaliana OX=3702 GN=At4g31860 ...	[more]
Q6ETK3	3.1e-11	75.61	Probable protein phosphatase 2C 11 OS=Oryza sativa subsp. japonica OX=39947 GN=O...	[more]
Q653S3	4.1e-11	76.92	Probable protein phosphatase 2C 70 OS=Oryza sativa subsp. japonica OX=39947 GN=O...	[more]

BLAST of Carg13500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7J7D6U7	2.0e-13	67.19	Protein-serine/threonine phosphatase OS=Tripterygium wilfordii OX=458696 GN=HS08...	[more]
A0A5N5NBL1	3.5e-13	59.49	Protein-serine/threonine phosphatase OS=Salix brachista OX=2182728 GN=DKX38_0049...	[more]
A0A6J1KZ81	6.0e-13	100.00	Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111497691...	[more]
A0A6J1HB24	6.0e-13	100.00	Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114611...	[more]
A0A6J1JMK1	1.0e-12	61.84	Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111488236...	[more]

BLAST of Carg13500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25070.1	7.0e-14	84.62	Protein phosphatase 2C family protein	[more]
AT4G31860.2	2.0e-13	82.05	Protein phosphatase 2C family protein	[more]
AT4G31860.1	2.0e-13	82.05	Protein phosphatase 2C family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036457	PPM-type phosphatase domain superfamily	GENE3D	3.60.40.10		coord: 34..95 e-value: 9.4E-10 score: 40.0
IPR036457	PPM-type phosphatase domain superfamily	SUPERFAMILY	81606	PP2C-like	coord: 38..77
IPR015655	Protein phosphatase 2C family	PANTHER	PTHR13832	PROTEIN PHOSPHATASE 2C	coord: 32..75
None	No IPR available	PANTHER	PTHR13832:SF744	PROTEIN PHOSPHATASE 2C 60 ISOFORM X1-RELATED	coord: 32..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13500-RA	Carg13500-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006470	protein dephosphorylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004722	protein serine/threonine phosphatase activity