Carg13466 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13466
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionBeta-glucosidase
LocationCarg_Chr08: 2965928 .. 2969559 (-)
RNA-Seq ExpressionCarg13466
SyntenyCarg13466
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCAGCGAGTAACGCTCCGGTGCTTCTGATAACGCTCATTGCGGCCGCAATTGCCGGCGGCGCTTTGGCTGATGGTGGGAGCAGCCCTTCTGTGGAGCCGAGTCATACTTCAGTTCCCTTCAACCGGAGCAGTTTCCCGGCGGGTTTTCTATTCGGAGCTGGCTCCGCCGCCTACCAGGTTTATCCCCTCTCTCTCTCTCTCTCTCTCCGCCTCTCTCTCCGCCGCCTTCCAGAGCACCGACATTGCCAGTTTTGTTTCTTTGCAGTTAGAAGGAGCAGCTAGTAAAGATGGAAGAGGTCCAAGTATTTGGGACACTTTCACTAAGAACCACCCCGGTCTGGTTCTTCATTACCAATAATTTTTATTTATGGCTAAATTAAAAACTTTAAATAGTTTAAATATAAAATAATGATATGCTACGATCGTATAGTTGACGGGTTATTGAATTAAATGATCAGAGAAAATATGGGACCATAGCAGTGGAGAAGTGGCTACCGATTTCTACCATCGTTATAAGGTAACATTTTTGCACTCTTATTTTTCACTATTAATTATATGACTAAAAAATTTAGCTAATATTTTTTAATATTTAAATATTTAGGAACTTATTATATATATTTTGTTATTATTAAAAGTAAATTAATAATATTGGATTATAATTATAATTATAATTATAATTATTAAAAAATTGTGATTTAATTTGTAGGAGGACGTGCAAATAATGAAAAAGATGGGGTTGGACTCTTTCAGATTCTCCATCTCTTGGTCAAGGATTCTCCCCAGTAAGTTTTTTTTTTTTTTTACCCAAAAGTTATTATTTCCATTTCCATCGGTAAATTTTTGTTTTTTACCGTTTTATTTTTCCTTCGAAAAAAAAATGACAGAGGGAACCGTTCGTGGAGGAGTGAATCCACTTGGCGTCAAATTTTACAACAATCTCATCAATGAGCTCCTCGCCAATGGTAAAAAATTTAAAAAGAGGTTAATTTTATTATTAAATTAATGTAAGAATTATATTTTTATATATCCTAAATAATTATTTGTGGTGAACAAAAAAAATATAGGAATAATACCGTACGTCACTCTTTTTCATTGGGATCTTCCTCAAGCACTTGAAGATAAGTACGATGGATTTCGAAACGTCAAAATTGTGTAAGTAAAATAATTAATTTTATTATATAAAATTAAAAGTTTACGATGTAATACACATAAAACTCAATTTTATATTTTAATTTAAAAATATTAAATATGAGTCAAATTTAAACATTAATTGTAATTAATTAAAAATTAATTAATGTAATTTTGGTGATTAAACAGGAATGATTTTCGGAATTACGCGGACTTGTGCTTCAAATTATTTGGCGATCGGGTCAAGTATTGGACAACGCTTAACGAACCATATTCATTTAGCGCTTTTGGGTATAACAGCGGCACTTTCGCTCCTGGAAGATGTTCCAACTACGTCGGAAATTGTACCGCCGGCAACTCCGGCACCGAGCCCTACATCGTCGCTCACAATCTCCTCCTCTCCCATGCCGCTGCCGTCAAAGTTTACAGGACAAAGTACCAGGTCAGTATTCAAGAACAAATCTCTAAATCAATCTCTTCATTGTTAATCAGATTCTTGTTTTGGACGCCGATTTTGCTAATTCGGTTTCATTTGAGAGCTACAGGAAAAGCAGAAGGGGAAGATTGGAATCACATTGGTGACTCACTGGTTTAGGCCCAAACGCAACACGCCAGCTTCGCGAGCGGCGGCGAATCGAGCTCTTGATTTCTTCTTGGGATGGTGAGTGTCTTGATCGTAATCGTTAGTGGAGAAATGTTAGTGAAAATTAAGTAACTGAGTTGAAATGGATTTTAGGTTTCTGCATCCGATTACTTATGGCACCTACCCTAAATCGATGCGTCAGTACGTCGGAGATAGACTGCCGAAATTCTCCGCGGCAGAGTCAAAAAGTGTTAAAGGATCGATGGATTTTCTTGGAATGAATTACTACACTGGGAATTTTGCTGACAATGTGCCTTTCTCAAATTCGCCCAACAAAAGCTATAGTTCTGATGCACACGTCGCATTCTCTAGTATGTCTTCTTTCTTTTCTTTGACGAGATAATTAAATTTTAGGGCGAACTTCGATCTTTACACCTAAATTTGAATAAGCTATGCAATTTCTATGTTAGGAATTCATGAAATATGATATTAGACGAGAGGTGAATTTTGATTGAGATTTGTGAAGTTTTAGAATTTTATTGAGCGAGTTCTTGAAGCTTGGTAGATGGTAGGTTGTTTTTTGTTTGAAAGTTTAGACTAAAATATGATTCAATTGTCAAGTTAAAGTGATTTTTCATCCGAACCAAACCTTTTTCTTATTCTTAACATGAATTTTTTCTTCTACAGCGGAGAGAGATGGTGTTCTAATTGGACCAGCGGTTCGTACTCTCTCTATCTATCCATCTAGAGCTGTTATTTTCATTACTTTAATTAGCAGCTGAGCGATTTATTTATGCTTCTGCAGACTGGTTTGAACTGGCTTTACATCTACCCAGAGGGTATTCGTCTACTTTTGAAATACGTTAAAGCAGAGTACAAAGATCCAGTTATTTACATCACCGAGAATGGTACCTCTCTTTCTCTCCAATCAATGGCTATACAACGATGCATTTAGTTAGTCATATGAAAAATGAAATTTGAAGACAAAAGGTCAAATAAAAAGTTTGTATTTCATATATGAGATCCCATGTCGGTTGGAGAGAGGAATGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCCTGGGAGAGGCGTTTCAAAAACCGTGAGGCTAATGGCGATATGTAATGGGTAAAAGTGAACAATATTTGTTAGTGGTGTACTTGGGCTGTTACAAATGGTATCAAAGCCAAACACCACACGGTGTGCTAGCGAGGACGCTAGGCTCCCGAGGAGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGGAAATGAAACATTTCTTATAAGGGTGTGGAAACGTCTCCCTAGTAGATGCATTTTAAAAACAGTGAGGTTGATAGCGATACGAAACGGACCAAAGCGGACAATATTTGCTAGCAGTAGACTTGGGCTGTTACAAATGATATCAAAGTCAGACACCGAACAATGTGTCAGTGAGGACACGCTGGGCTCCAAAGGGGGTGGATTGTGAGATCCCACATCGGCGGTTGGAGAGGGGAAGGAATCATTTTTTATAAGGGTGTGGAAACCTCTCCCTAGTAAACACGTTTTAAAAACTGTGAGGCTGGCGGTGATACGTAACGGACCAAAGCGGACAATATTTGTTACTTGTGGACTTAGACTATTACAATCATATTTACCGCGTTTTTTGGATGTATTTGGAGATAGGTTTAGAAATGATTAGAAATGTGTTTGGTTGAATCTCATTGTTTATCAATTTATGAATGAATATGTTTTCTGCTAAATGGATACTAGACAAGCCCTAAACTATTGGGTTGTTGTGGTGTACAGGTATGGCTTATTCAGACAATATGACACTGCCAATTAAGGAAGCTCTAAAAGATGGAACAAGGATCAAATACCACCACGCCCATCTCGCATCTCTTCTTCAAGCTATCAAGTATGAATGA

mRNA sequence

ATGGCGGCAGCGAGTAACGCTCCGGTGCTTCTGATAACGCTCATTGCGGCCGCAATTGCCGGCGGCGCTTTGGCTGATGGTGGGAGCAGCCCTTCTGTGGAGCCGAGTCATACTTCAGTTCCCTTCAACCGGAGCAGTTTCCCGGCGGGTTTTCTATTCGGAGCTGGCTCCGCCGCCTACCAGTTAGAAGGAGCAGCTAGTAAAGATGGAAGAGGTCCAAGTATTTGGGACACTTTCACTAAGAACCACCCCGAGAAAATATGGGACCATAGCAGTGGAGAAGTGGCTACCGATTTCTACCATCGTTATAAGGAGGACGTGCAAATAATGAAAAAGATGGGGTTGGACTCTTTCAGATTCTCCATCTCTTGGTCAAGGATTCTCCCCAAGGGAACCGTTCGTGGAGGAGTGAATCCACTTGGCGTCAAATTTTACAACAATCTCATCAATGAGCTCCTCGCCAATGTTGAAATGGATTTTAGGTTTCTGCATCCGATTACTTATGGCACCTACCCTAAATCGATGCGTCAGTACGTCGGAGATAGACTGCCGAAATTCTCCGCGGCAGAGTCAAAAAGTGTTAAAGGATCGATGGATTTTCTTGGAATGAATTACTACACTGGGAATTTTGCTGACAATGTGCCTTTCTCAAATTCGCCCAACAAAAGCTATAGTTCTGATGCACACGTCGCATTCTCTACGGAGAGAGATGGTGTTCTAATTGGACCAGCGGTTCGTATGGCTTATTCAGACAATATGACACTGCCAATTAAGGAAGCTCTAAAAGATGGAACAAGGATCAAATACCACCACGCCCATCTCGCATCTCTTCTTCAAGCTATCAAGTATGAATGA

Coding sequence (CDS)

ATGGCGGCAGCGAGTAACGCTCCGGTGCTTCTGATAACGCTCATTGCGGCCGCAATTGCCGGCGGCGCTTTGGCTGATGGTGGGAGCAGCCCTTCTGTGGAGCCGAGTCATACTTCAGTTCCCTTCAACCGGAGCAGTTTCCCGGCGGGTTTTCTATTCGGAGCTGGCTCCGCCGCCTACCAGTTAGAAGGAGCAGCTAGTAAAGATGGAAGAGGTCCAAGTATTTGGGACACTTTCACTAAGAACCACCCCGAGAAAATATGGGACCATAGCAGTGGAGAAGTGGCTACCGATTTCTACCATCGTTATAAGGAGGACGTGCAAATAATGAAAAAGATGGGGTTGGACTCTTTCAGATTCTCCATCTCTTGGTCAAGGATTCTCCCCAAGGGAACCGTTCGTGGAGGAGTGAATCCACTTGGCGTCAAATTTTACAACAATCTCATCAATGAGCTCCTCGCCAATGTTGAAATGGATTTTAGGTTTCTGCATCCGATTACTTATGGCACCTACCCTAAATCGATGCGTCAGTACGTCGGAGATAGACTGCCGAAATTCTCCGCGGCAGAGTCAAAAAGTGTTAAAGGATCGATGGATTTTCTTGGAATGAATTACTACACTGGGAATTTTGCTGACAATGTGCCTTTCTCAAATTCGCCCAACAAAAGCTATAGTTCTGATGCACACGTCGCATTCTCTACGGAGAGAGATGGTGTTCTAATTGGACCAGCGGTTCGTATGGCTTATTCAGACAATATGACACTGCCAATTAAGGAAGCTCTAAAAGATGGAACAAGGATCAAATACCACCACGCCCATCTCGCATCTCTTCTTCAAGCTATCAAGTATGAATGA

Protein sequence

MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNNLINELLANVEMDFRFLHPITYGTYPKSMRQYVGDRLPKFSAAESKSVKGSMDFLGMNYYTGNFADNVPFSNSPNKSYSSDAHVAFSTERDGVLIGPAVRMAYSDNMTLPIKEALKDGTRIKYHHAHLASLLQAIKYE
Homology
BLAST of Carg13466 vs. NCBI nr
Match: KAG7025675.1 (hypothetical protein SDJN02_12173, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.5 bits (1469), Expect = 8.3e-159
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANVEMDFRFLHPITYGTYPKSMRQYVG 180
           SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANVEMDFRFLHPITYGTYPKSMRQYVG
Sbjct: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANVEMDFRFLHPITYGTYPKSMRQYVG 180

Query: 181 DRLPKFSAAESKSVKGSMDFLGMNYYTGNFADNVPFSNSPNKSYSSDAHVAFSTERDGVL 240
           DRLPKFSAAESKSVKGSMDFLGMNYYTGNFADNVPFSNSPNKSYSSDAHVAFSTERDGVL
Sbjct: 181 DRLPKFSAAESKSVKGSMDFLGMNYYTGNFADNVPFSNSPNKSYSSDAHVAFSTERDGVL 240

Query: 241 IGPAVRMAYSDNMTLPIKEALKDGTRIKYHHAHLASLLQAIKYE 285
           IGPAVRMAYSDNMTLPIKEALKDGTRIKYHHAHLASLLQAIKYE
Sbjct: 241 IGPAVRMAYSDNMTLPIKEALKDGTRIKYHHAHLASLLQAIKYE 284

BLAST of Carg13466 vs. NCBI nr
Match: XP_022960187.1 (vicianin hydrolase-like [Cucurbita moschata])

HSP 1 Score: 472.6 bits (1215), Expect = 2.3e-129
Identity = 276/462 (59.74%), Postives = 278/462 (60.17%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFT+NHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTRNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQALEDKYDGFR 180

Query: 181 ---VEMDFR--------------------------------------------------- 240
              +  DFR                                                   
Sbjct: 181 NVKIVNDFRNYADLCFKLFGDRVKYWTTLNEPYSFSAFGYNSGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSGTEPYIVAHNLLLSHAAAVKVYRTKYQAKQKGKIGITLVTHWFRPKRNTPASRAAANR 300

BLAST of Carg13466 vs. NCBI nr
Match: XP_023513690.1 (vicianin hydrolase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.3 bits (1209), Expect = 1.2e-128
Identity = 274/462 (59.31%), Postives = 278/462 (60.17%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQALEDKYDGFR 180

Query: 181 ---VEMDFR--------------------------------------------------- 240
              +  DFR                                                   
Sbjct: 181 NVKIVNDFRNYADLCFKLFGDRVKYWTTLNEPYSFSAYGYNSGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSGTEPYIVAHNLLLSHAAAVKVYRTRYQAKQKGKIGITLVTHWFRPKRNTPASRAAANR 300

BLAST of Carg13466 vs. NCBI nr
Match: XP_023004521.1 (vicianin hydrolase-like [Cucurbita maxima])

HSP 1 Score: 456.4 bits (1173), Expect = 1.7e-124
Identity = 265/462 (57.36%), Postives = 275/462 (59.52%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADG +SPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGRNSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKG++RGGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SISWSRILPKGSIRGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQALEDEYNGFR 180

Query: 181 -----------VEMDFR------------------------------------------- 240
                       ++ F+                                           
Sbjct: 181 NVKIVNDFGNYADLCFKLFGDRVKYWTTLNEPYSFSVFGYNSGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSGTEPYIVAHNLLLAHAAAVKVYRTKYQAKQKGKIGITLVTHWFRPKRNTPASRAAANR 300

BLAST of Carg13466 vs. NCBI nr
Match: XP_004135908.1 (vicianin hydrolase [Cucumis sativus] >KGN45162.1 hypothetical protein Csa_016147 [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 5.3e-113
Identity = 243/461 (52.71%), Postives = 260/461 (56.40%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           M AA NAPVLLITLIAA+IA GAL  GG+SP VEPSHTSVPFNRSSFPAGF+FGAGSAAY
Sbjct: 1   MTAAYNAPVLLITLIAASIASGALGTGGTSPPVEPSHTSVPFNRSSFPAGFIFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAAS DGRGPSIWDTFTKNHPEKIWDHSSGE ATDFYHRYKED+++MK MGLDSFRF
Sbjct: 61  QLEGAASLDGRGPSIWDTFTKNHPEKIWDHSSGERATDFYHRYKEDIKLMKLMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKG +RGG+NPLGVKFYNN+INELLAN                         
Sbjct: 121 SISWSRILPKGKIRGGINPLGVKFYNNVINELLANKIVPYVTLFHWDLPQALEDEYGGFL 180

Query: 181 ---VEMDFR--------------------------------------------------- 240
              V  DFR                                                   
Sbjct: 181 SSKVVNDFREYVDLCFKLFGDRVKYWVTLNEPFSYSFNGYNGGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 282
                                                                       
Sbjct: 241 NSATEPYIVAHNLLLSHSAAVKLYKQKYQKKQKGQIGITLVTHWFRPKRNTAASQKAANR 300

BLAST of Carg13466 vs. ExPASy Swiss-Prot
Match: A2SY66 (Vicianin hydrolase (Fragment) OS=Vicia sativa subsp. nigra OX=3909 PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.1e-60
Identity = 157/456 (34.43%), Postives = 195/456 (42.76%), Query Frame = 0

Query: 7   APVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAYQLEGAA 66
           +P LL     A +       G  S  V PSH +  FN+S FP  FLFG GS+AYQ+EGA+
Sbjct: 2   SPSLLYLFSLATLLAVVTGTGTPSQEVHPSHYATTFNKSLFPKDFLFGIGSSAYQVEGAS 61

Query: 67  SKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSR 126
           + DGRGPSIWDTFTK HPEKIWDHSSG +  DFYHRYK D++I+K++GLDS+RFSISWSR
Sbjct: 62  NIDGRGPSIWDTFTKQHPEKIWDHSSGNIGADFYHRYKSDIKIVKEIGLDSYRFSISWSR 121

Query: 127 ILPKGTVRGGVNPLGVKFYNNLINELLAN----------------------------VEM 186
           I PKG  +G VNPLGVKFYNN+INE+LAN                            V  
Sbjct: 122 IFPKG--KGEVNPLGVKFYNNVINEILANGLIPFVTLFHWDLPQSLEDEYKGFLSSKVVK 181

Query: 187 DFR--------------------------------------------------------- 246
           DF                                                          
Sbjct: 182 DFENYADFVFKTYGDRVKHWVTLNEPFSYALYGYNGGTFAPGRCSKYAGNCEYGDSSTEP 241

Query: 247 ------------------------------------------------------------ 283
                                                                       
Sbjct: 242 YIVAHNLILSHAAAAKLYKTKYQAHQKGNIGATLVTHYFEPHSNSAADRVAASRALDFFF 301

BLAST of Carg13466 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.5e-59
Identity = 158/465 (33.98%), Postives = 207/465 (44.52%), Query Frame = 0

Query: 1   MAAASNAP--VLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSA 60
           MAAA   P  +LL  L+ A +A GA    G     EP     P +R SFP GF+FG  S+
Sbjct: 1   MAAAGAMPGGLLLTFLLLAVVASGAYNSAG-----EP-----PVSRRSFPKGFIFGTASS 60

Query: 61  AYQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSF 120
           +YQ EG A++ GRGPSIWDTFT  HPEKI D S+G+VA+D YH YKEDV++MK MG+D++
Sbjct: 61  SYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAY 120

Query: 121 RFSISWSRILPKGTVRGGVNPLGVKFYNNLINELLA------------------------ 180
           RFSISW+RILP G++RGGVN  G+K+YNNLINELL+                        
Sbjct: 121 RFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNG 180

Query: 181 ----NVEMDFR------------------------------------------------- 240
               N+  DF+                                                 
Sbjct: 181 FLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNC 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 SVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDA 300

BLAST of Carg13466 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 5.9e-59
Identity = 158/465 (33.98%), Postives = 207/465 (44.52%), Query Frame = 0

Query: 1   MAAASNAP--VLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSA 60
           MAAA   P  +LL  L+ A +A GA    G     EP     P +R SFP GF+FG  S+
Sbjct: 1   MAAAGAMPGGLLLTFLLLAVVASGAYNGAG-----EP-----PVSRRSFPKGFIFGTASS 60

Query: 61  AYQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSF 120
           +YQ EG A++ GRGPSIWDTFT  HPEKI D S+G+VA+D YH YKEDV++MK MG+D++
Sbjct: 61  SYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAY 120

Query: 121 RFSISWSRILPKGTVRGGVNPLGVKFYNNLINELLA------------------------ 180
           RFSISW+RILP G++RGGVN  G+K+YNNLINELL+                        
Sbjct: 121 RFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNG 180

Query: 181 ----NVEMDFR------------------------------------------------- 240
               N+  DF+                                                 
Sbjct: 181 FLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNC 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 SVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDA 300

BLAST of Carg13466 vs. ExPASy Swiss-Prot
Match: Q7XKV2 (Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 2.6e-54
Identity = 139/423 (32.86%), Postives = 185/423 (43.74%), Query Frame = 0

Query: 41  PFNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFY 100
           P +R SFP GF+FG  S++YQ EG A + GRGPSIWDTFT  HP+KI D S+G+VA D Y
Sbjct: 29  PISRRSFPEGFIFGTASSSYQYEGGAREGGRGPSIWDTFTHQHPDKIADKSNGDVAADSY 88

Query: 101 HRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNNLINELL------- 160
           H YKEDV+IMK MG+D++RFSISW+RILP G++ GG+N  G+ +YNNLINELL       
Sbjct: 89  HLYKEDVRIMKDMGVDAYRFSISWTRILPNGSLSGGINREGISYYNNLINELLLKGVQPF 148

Query: 161 ------------------------------------------------------------ 220
                                                                       
Sbjct: 149 VTLFHWDSPQALEDKYNGFLSPNIINDYKEYAETCFKEFGDRVKHWITFNEPLSFCVAGY 208

Query: 221 ------------------------------------------------------------ 280
                                                                       
Sbjct: 209 ASGGMFAPGRCSPWEGNCSAGDSGREPYTACHHQLLAHAETVRLYKEKYQVLQKGKIGIT 268

Query: 281 -------------ANVE-----MDFR---FLHPITYGTYPKSMRQYVGDRLPKFSAAESK 283
                        +N++     +DF    F+ P+  G YP SMR+ V +RLP+F+  +S+
Sbjct: 269 LVSNWFVPFSRSKSNIDAARRALDFMLGWFMDPLIRGEYPLSMRELVRNRLPQFTKEQSE 328

BLAST of Carg13466 vs. ExPASy Swiss-Prot
Match: O64882 (Beta-glucosidase 17 OS=Arabidopsis thaliana OX=3702 GN=BGLU17 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.2e-53
Identity = 142/432 (32.87%), Postives = 190/432 (43.98%), Query Frame = 0

Query: 32  SVEPS--HTSVPFNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKIWD 91
           +++PS    S    RSSFP  F FGA S+AYQ EGAA+ DGR PSIWDTFTK +PEKI D
Sbjct: 23  ALDPSFLRLSTSLQRSSFPQDFRFGAASSAYQSEGAANVDGREPSIWDTFTKQYPEKISD 82

Query: 92  HSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNNLI 151
            S+G+VA +FY+R+KEDV  MK++GLDSFRFSISWSRILP+GTV GGVN  G+ FYN+LI
Sbjct: 83  GSNGDVADEFYYRFKEDVAHMKEIGLDSFRFSISWSRILPRGTVAGGVNQAGINFYNHLI 142

Query: 152 NELLAN------------------------------------VEMDFR------------ 211
           NEL++N                                    V++ F+            
Sbjct: 143 NELISNGIRPLVTLFHWDTPQALEDEYGGFLNPQIVKDFVEYVDICFKEFGDRVKEWITI 202

Query: 212 ------------------------------------------------------------ 271
                                                                       
Sbjct: 203 NEPNMFAVLGYNVGNIAPGRCSSYVQNCTVGNSATEPYLVAHYLILSHAATVQLYREKYQ 262

Query: 272 ---------------------------------------FLHPITYGTYPKSMRQYVGDR 282
                                                  F  PITYG YPK+MR+ VG+R
Sbjct: 263 SFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDFFFGWFADPITYGDYPKTMRELVGNR 322

BLAST of Carg13466 vs. ExPASy TrEMBL
Match: A0A6J1H6Q8 (vicianin hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC111460999 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.1e-129
Identity = 276/462 (59.74%), Postives = 278/462 (60.17%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFT+NHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTRNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQALEDKYDGFR 180

Query: 181 ---VEMDFR--------------------------------------------------- 240
              +  DFR                                                   
Sbjct: 181 NVKIVNDFRNYADLCFKLFGDRVKYWTTLNEPYSFSAFGYNSGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSGTEPYIVAHNLLLSHAAAVKVYRTKYQAKQKGKIGITLVTHWFRPKRNTPASRAAANR 300

BLAST of Carg13466 vs. ExPASy TrEMBL
Match: A0A6J1KSC1 (vicianin hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111497798 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.4e-125
Identity = 265/462 (57.36%), Postives = 275/462 (59.52%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAASNAPVLLITLIAAAIAGGALADG +SPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY
Sbjct: 1   MAAASNAPVLLITLIAAAIAGGALADGRNSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF
Sbjct: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKG++RGGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SISWSRILPKGSIRGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQALEDEYNGFR 180

Query: 181 -----------VEMDFR------------------------------------------- 240
                       ++ F+                                           
Sbjct: 181 NVKIVNDFGNYADLCFKLFGDRVKYWTTLNEPYSFSVFGYNSGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSGTEPYIVAHNLLLAHAAAVKVYRTKYQAKQKGKIGITLVTHWFRPKRNTPASRAAANR 300

BLAST of Carg13466 vs. ExPASy TrEMBL
Match: A0A0A0K9V3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G428990 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.5e-113
Identity = 243/461 (52.71%), Postives = 260/461 (56.40%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           M AA NAPVLLITLIAA+IA GAL  GG+SP VEPSHTSVPFNRSSFPAGF+FGAGSAAY
Sbjct: 1   MTAAYNAPVLLITLIAASIASGALGTGGTSPPVEPSHTSVPFNRSSFPAGFIFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAAS DGRGPSIWDTFTKNHPEKIWDHSSGE ATDFYHRYKED+++MK MGLDSFRF
Sbjct: 61  QLEGAASLDGRGPSIWDTFTKNHPEKIWDHSSGERATDFYHRYKEDIKLMKLMGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           SISWSRILPKG +RGG+NPLGVKFYNN+INELLAN                         
Sbjct: 121 SISWSRILPKGKIRGGINPLGVKFYNNVINELLANKIVPYVTLFHWDLPQALEDEYGGFL 180

Query: 181 ---VEMDFR--------------------------------------------------- 240
              V  DFR                                                   
Sbjct: 181 SSKVVNDFREYVDLCFKLFGDRVKYWVTLNEPFSYSFNGYNGGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 282
                                                                       
Sbjct: 241 NSATEPYIVAHNLLLSHSAAVKLYKQKYQKKQKGQIGITLVTHWFRPKRNTAASQKAANR 300

BLAST of Carg13466 vs. ExPASy TrEMBL
Match: A0A6J1D5L7 (vicianin hydrolase-like OS=Momordica charantia OX=3673 GN=LOC111017519 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 6.5e-109
Identity = 235/462 (50.87%), Postives = 261/462 (56.49%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAY 60
           MAAA+ APVLLI+LIAAA+AGGALADG +SPSVEPSH++VPFNRSSFPAGF+FGAGSAAY
Sbjct: 1   MAAANIAPVLLISLIAAALAGGALADGTASPSVEPSHSTVPFNRSSFPAGFVFGAGSAAY 60

Query: 61  QLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRF 120
           QLEGAA  DGRGPSIWDTFTK+HPEKIWDHSSG+VATDFYHRYK+DV+IMK +GLDSFRF
Sbjct: 61  QLEGAAHTDGRGPSIWDTFTKDHPEKIWDHSSGDVATDFYHRYKDDVKIMKMLGLDSFRF 120

Query: 121 SISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------- 180
           S+SWSRILPKG + GGVNPLGVKFYNNLINELLAN                         
Sbjct: 121 SLSWSRILPKGKLSGGVNPLGVKFYNNLINELLANGIIPYVTLFHWDLPQTLEDEYGGFL 180

Query: 181 -----------VEMDFR------------------------------------------- 240
                      V++ F+                                           
Sbjct: 181 SPKVVDDYRDYVDLCFKLFGDRVKYWVTLNEPLSYSNNGYNTGTFAPGRCSNYVGNCTAG 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 NSATEPYIVSHHLILSHAAAVEVYKKKYQAKQKGQIGVTLVTHWFRPKRNTAASKRAASR 300

BLAST of Carg13466 vs. ExPASy TrEMBL
Match: A0A1S3CFH7 (vicianin hydrolase-like OS=Cucumis melo OX=3656 GN=LOC103499893 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 5.5e-108
Identity = 235/463 (50.76%), Postives = 257/463 (55.51%), Query Frame = 0

Query: 1   MAAASNAPVLLITLIAAAIAGGALADGG-SSPSVEPSHTSVPFNRSSFPAGFLFGAGSAA 60
           M AA NAPVLLITLIAAAIA GAL   G +SPSVEPSHTSVPFNRSSFPAGF+FGAGSAA
Sbjct: 1   MTAAYNAPVLLITLIAAAIANGALGTAGTTSPSVEPSHTSVPFNRSSFPAGFIFGAGSAA 60

Query: 61  YQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFR 120
           YQLEGAAS DGRGPSIWDTFTKNHPEKIWD  +GE ATDFYHRYKED+++MK +GLDSFR
Sbjct: 61  YQLEGAASLDGRGPSIWDTFTKNHPEKIWDRKTGERATDFYHRYKEDIKLMKLLGLDSFR 120

Query: 121 FSISWSRILPKGTVRGGVNPLGVKFYNNLINELLAN------------------------ 180
           FSISWSRILPKG +RGG+NPLGVKFYNN+INELLAN                        
Sbjct: 121 FSISWSRILPKGKIRGGINPLGVKFYNNVINELLANGIVPYVTLFHWDLPQALEDEYGGF 180

Query: 181 ----VEMDFR-------------------------------------------------- 240
               V  DFR                                                  
Sbjct: 181 LSTKVVNDFREYADLCFKLFGDRVKYWVTLNEPYSYSSNGYNGGTFAPGRCSNYVGNCTA 240

Query: 241 ------------------------------------------------------------ 283
                                                                       
Sbjct: 241 GNSATEPYIVAHNLLLSHSAAVKLYKQKYQKKQKGQIGITVVTHWFRPRRNTPASQRAAY 300

BLAST of Carg13466 vs. TAIR 10
Match: AT2G44480.1 (beta glucosidase 17 )

HSP 1 Score: 210.7 bits (535), Expect = 1.5e-54
Identity = 142/432 (32.87%), Postives = 190/432 (43.98%), Query Frame = 0

Query: 32  SVEPS--HTSVPFNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKIWD 91
           +++PS    S    RSSFP  F FGA S+AYQ EGAA+ DGR PSIWDTFTK +PEKI D
Sbjct: 23  ALDPSFLRLSTSLQRSSFPQDFRFGAASSAYQSEGAANVDGREPSIWDTFTKQYPEKISD 82

Query: 92  HSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNNLI 151
            S+G+VA +FY+R+KEDV  MK++GLDSFRFSISWSRILP+GTV GGVN  G+ FYN+LI
Sbjct: 83  GSNGDVADEFYYRFKEDVAHMKEIGLDSFRFSISWSRILPRGTVAGGVNQAGINFYNHLI 142

Query: 152 NELLAN------------------------------------VEMDFR------------ 211
           NEL++N                                    V++ F+            
Sbjct: 143 NELISNGIRPLVTLFHWDTPQALEDEYGGFLNPQIVKDFVEYVDICFKEFGDRVKEWITI 202

Query: 212 ------------------------------------------------------------ 271
                                                                       
Sbjct: 203 NEPNMFAVLGYNVGNIAPGRCSSYVQNCTVGNSATEPYLVAHYLILSHAATVQLYREKYQ 262

Query: 272 ---------------------------------------FLHPITYGTYPKSMRQYVGDR 282
                                                  F  PITYG YPK+MR+ VG+R
Sbjct: 263 SFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDFFFGWFADPITYGDYPKTMRELVGNR 322

BLAST of Carg13466 vs. TAIR 10
Match: AT5G42260.1 (beta glucosidase 12 )

HSP 1 Score: 171.4 bits (433), Expect = 1.0e-42
Identity = 115/357 (32.21%), Postives = 154/357 (43.14%), Query Frame = 0

Query: 37  HTSVP-FNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKIWDHSSGEV 96
           H+S P   RS FP  F+FGA ++AYQ+EGAA +DGRGPSIWDTF++ +PEKI D S+G +
Sbjct: 25  HSSTPKLRRSDFPEDFIFGAATSAYQVEGAAHEDGRGPSIWDTFSEKYPEKIKDGSNGSI 84

Query: 97  ATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNNLINELL-- 156
           A+D YH YKEDV ++ ++G D++RFSISWSRILP+  ++GG+N  G+ +YNNLINELL  
Sbjct: 85  ASDSYHLYKEDVGLLHQIGFDAYRFSISWSRILPRENLKGGINQAGIDYYNNLINELLSK 144

Query: 157 --------------------------ANVEMDFR-------------------------- 216
                                     A +  DFR                          
Sbjct: 145 GIKPFATIFHWDTPQSLEDAYGGFLGAEIVNDFRDYADICFKNFGDRVKHWMTLNEPLTV 204

Query: 217 ------------------------------------------------------------ 244
                                                                       
Sbjct: 205 VQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLILAHGEAVKVYREKYKASQKG 264

BLAST of Carg13466 vs. TAIR 10
Match: AT5G44640.1 (beta glucosidase 13 )

HSP 1 Score: 168.7 bits (426), Expect = 6.8e-42
Identity = 114/365 (31.23%), Postives = 157/365 (43.01%), Query Frame = 0

Query: 29  SSPSVEPSHTSVP-FNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKI 88
           S+  +   H+S P   RS FP  F+FGA ++AYQ+EGAA +DGRGPSIWDTF++ +PEKI
Sbjct: 17  SNEVIAKKHSSTPKLRRSDFPKDFIFGAATSAYQVEGAAHEDGRGPSIWDTFSEKYPEKI 76

Query: 89  WDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNN 148
            D ++G +A+D YH YKEDV ++ ++G  ++RFSISWSRILP+G ++GG+N  G+ +YNN
Sbjct: 77  KDGTNGSIASDSYHLYKEDVGLLHQIGFGAYRFSISWSRILPRGNLKGGINQAGIDYYNN 136

Query: 149 LINELL----------------------------ANVEMDFR------------------ 208
           LINELL                            A +  DFR                  
Sbjct: 137 LINELLSKGIKPFATIFHWDTPQSLEDAYGGFFGAEIVNDFRDYADICFKNFGDRVKHWM 196

Query: 209 ------------------------------------------------------------ 244
                                                                       
Sbjct: 197 TLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLILAHGEAVKVYRE 256

BLAST of Carg13466 vs. TAIR 10
Match: AT2G25630.1 (beta glucosidase 14 )

HSP 1 Score: 166.4 bits (420), Expect = 3.4e-41
Identity = 123/415 (29.64%), Postives = 172/415 (41.45%), Query Frame = 0

Query: 29  SSPSVEPSHTSVP-FNRSSFPAGFLFGAGSAAYQLEGAASKDGRGPSIWDTFTKNHPEKI 88
           S+  V   H+S P   ++ FP  F+FGA ++AYQ+EGAA +DGRGPSIWDTF++ +PEKI
Sbjct: 16  SNEVVAKRHSSTPKLRKTDFPEDFIFGAATSAYQVEGAAQEDGRGPSIWDTFSEKYPEKI 75

Query: 89  WDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILPKGTVRGGVNPLGVKFYNN 148
            D S+G +A D YH YKEDV ++ ++G +++RFSISWSRILP+G ++GG+N  G+ +YNN
Sbjct: 76  KDGSNGSIADDSYHLYKEDVGLLHQIGFNAYRFSISWSRILPRGNLKGGINQAGIDYYNN 135

Query: 149 LINELL----------------------------ANVEMDFR------------------ 208
           LINELL                            A +  DFR                  
Sbjct: 136 LINELLSKGIKPFATIFHWDTPQDLEDAYGGFRGAEIVNDFRDYADICFKSFGDRVKHWI 195

Query: 209 ------------------------------------------------------------ 268
                                                                       
Sbjct: 196 TLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTAGNGATEPYIVGHNLILAHGEAIKVYRK 255

Query: 269 ------------------------------------------FLHPITYGTYPKSMRQYV 282
                                                     F+ P+  G YP  M   V
Sbjct: 256 KYKASQKGQVGIALNAGWNLPYTESAEDRLAAARAMAFTFDYFMEPLVTGKYPVDMVNNV 315

BLAST of Carg13466 vs. TAIR 10
Match: AT2G44450.1 (beta glucosidase 15 )

HSP 1 Score: 161.8 bits (408), Expect = 8.3e-40
Identity = 132/452 (29.20%), Postives = 181/452 (40.04%), Query Frame = 0

Query: 10  LLITLIAAAIAGGALADGGSSPSVEPSHTSVPFNRSSFPAGFLFGAGSAAYQLEGAASKD 69
           LL+ LI  A +   LA+  SS        +    RS FP  F+FG+ ++AYQ+EG A +D
Sbjct: 8   LLVVLIVLA-SNDVLANNNSS--------TPKLRRSDFPEDFIFGSATSAYQVEGGAHED 67

Query: 70  GRGPSIWDTFTKNHPEKIWDHSSGEVATDFYHRYKEDVQIMKKMGLDSFRFSISWSRILP 129
           GRGPSIWDTF++ +PEKI D S+G VA + YH YKEDV ++ ++G +++RFSISWSRILP
Sbjct: 68  GRGPSIWDTFSEKYPEKIKDGSNGSVADNSYHLYKEDVALLHQIGFNAYRFSISWSRILP 127

Query: 130 KGTVRGGVNPLGVKFYNNLINELL----------------------------ANVEMDFR 189
           +G ++GG+N  G+ +YNNLINELL                            A +  DFR
Sbjct: 128 RGNLKGGINQAGIDYYNNLINELLSKGIKPFATMFHWDTPQALEDAYGGFRGAEIVNDFR 187

Query: 190 ------------------------------------------------------------ 249
                                                                       
Sbjct: 188 DYADICFKNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSKFTNPNCTDGNGATEPYI 247

Query: 250 ------------------------------------------------------------ 282
                                                                       
Sbjct: 248 VGHNLILSHGAAVQVYREKYKASQQGQVGIALNAGWNLPYTESPKDRLAAARAMAFTFDY 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025675.18.3e-159100.00hypothetical protein SDJN02_12173, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022960187.12.3e-12959.74vicianin hydrolase-like [Cucurbita moschata][more]
XP_023513690.11.2e-12859.31vicianin hydrolase-like [Cucurbita pepo subsp. pepo][more]
XP_023004521.11.7e-12457.36vicianin hydrolase-like [Cucurbita maxima][more]
XP_004135908.15.3e-11352.71vicianin hydrolase [Cucumis sativus] >KGN45162.1 hypothetical protein Csa_016147... [more]
Match NameE-valueIdentityDescription
A2SY664.1e-6034.43Vicianin hydrolase (Fragment) OS=Vicia sativa subsp. nigra OX=3909 PE=1 SV=1[more]
B8AVF04.5e-5933.98Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1[more]
Q7XKV45.9e-5933.98Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2[more]
Q7XKV22.6e-5432.86Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2[more]
O648822.2e-5332.87Beta-glucosidase 17 OS=Arabidopsis thaliana OX=3702 GN=BGLU17 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H6Q81.1e-12959.74vicianin hydrolase-like OS=Cucurbita moschata OX=3662 GN=LOC111460999 PE=3 SV=1[more]
A0A6J1KSC18.4e-12557.36vicianin hydrolase-like OS=Cucurbita maxima OX=3661 GN=LOC111497798 PE=3 SV=1[more]
A0A0A0K9V32.5e-11352.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G428990 PE=3 SV=1[more]
A0A6J1D5L76.5e-10950.87vicianin hydrolase-like OS=Momordica charantia OX=3673 GN=LOC111017519 PE=3 SV=1[more]
A0A1S3CFH75.5e-10850.76vicianin hydrolase-like OS=Cucumis melo OX=3656 GN=LOC103499893 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44480.11.5e-5432.87beta glucosidase 17 [more]
AT5G42260.11.0e-4232.21beta glucosidase 12 [more]
AT5G44640.16.8e-4231.23beta glucosidase 13 [more]
AT2G25630.13.4e-4129.64beta glucosidase 14 [more]
AT2G44450.18.3e-4029.20beta glucosidase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001360Glycoside hydrolase family 1PFAMPF00232Glyco_hydro_1coord: 45..155
e-value: 1.3E-46
score: 159.0
coord: 162..228
e-value: 6.6E-10
score: 38.0
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 36..155
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 247..282
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 162..244
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 157..284
e-value: 1.4E-24
score: 88.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..156
e-value: 4.6E-54
score: 185.5
NoneNo IPR availablePANTHERPTHR10353:SF261BETA-GLUCOSIDASE 17coord: 36..155
NoneNo IPR availablePANTHERPTHR10353:SF261BETA-GLUCOSIDASE 17coord: 247..282
NoneNo IPR availablePANTHERPTHR10353:SF261BETA-GLUCOSIDASE 17coord: 162..244
IPR033132Glycosyl hydrolases family 1, N-terminal conserved sitePROSITEPS00653GLYCOSYL_HYDROL_F1_2coord: 51..65
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 38..155
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 160..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13466-RACarg13466-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds