Carg13463 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13463
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description30S ribosomal protein 2, chloroplastic-like
LocationCarg_Chr08: 2948442 .. 2951399 (+)
RNA-Seq ExpressionCarg13463
SyntenyCarg13463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCTTGTCGCTTCGGAACGCAAAGCTTTACAGACCAAAAATGGCTTCCATCTCTGCCGTGTTATCGCCAACGAATCTCATCCACAGTTCCACAAAACCCGCCGAAATCCTTCTCAAATCAGTCTCATTTCACCGCCGTCATACCCATACCCTCCTCCGCCTATCTCCCGGTCGCCCCACGAGACTCTTCGCGGCGCTTACTGCTCAGGATTCCAGTTCAGTGGCGGAAAGGAAATTGTACGTTGGAAATATACCTAGAAATGTGGATAATGAGGAGCTCAAGCGGATTGTTGAACAACATGGGGCTGTGGAGAAGGCTGAGGTGCGTAATTTATGATTGATTATTTATTATTATTTTTTTTTCTTCTATTGTACATTTTGAAATTTGTTGCGTACCCTACGCGTCGAGATGTTGATGTTGAGGTTTGCTTGCTGGATTCTATTATTGAGGACTTATATCTATCTGCTTGATTAGTTTGTCTTCAACGAATCTTCGCATGAATTGATTTGAGTTAATTGTTCATAAGTTAAGTTCATCGAAACTTCTTCCTGAATGTACCCTGATTTTGGCATGATTTAATCGAGTTAATTGTTTATAAGTTGAGTTCATCAAAACATCTGCCTGAACGTACCCTGATTTTGGGCCAAATTTGTCCACCATATACTTTCAAATGGAGTTTACTTGCAGAGTGTTATCCAATCCACGAAGGAATTAAAGATGGAAAATGTTCATAGGAGAGGGAAGGAAGAGTTCATAGGAAGGGAAAGGATAGTCACGCTCTGTTAGTTACAATAAGCTGCAGCCACTTATCAAAGGGAAGAAGGATGTACAGGACGATTTTAGGGGAAAGGAGAATCGAATCCTGTGAAATGCTCGCCACTGGCTCATAGAAATTCCATAGGACTTCAAAATTTAATTCTTGCTGCTTTCGGGTTCATTGCTTCAGTGTCGTTGCGTTTGTATGAGAGTATCTAAGATGAGTACCAAACAGTTCCTGAAATGAAGTAGCTAATGGCATGTCTTTAATGATGTATTTCTTGCAATCAATTTACTTGAAGAATTCTAGCATCTTCTTGTTCTTAATCTTTACTTGCTAACTGTTGGTATTGGCTTTAAAACTCCAATTTTGTTCCATTTCCAGGTCATGTATGACAAATACTCTGGAAGAAGCCGCAGGTTTGCCTTTGTTACCATGAAAACGGTTGACGACGCAAATGCAGCAATTGAGAAACTGAATGAGACAGTAAGCTAAATACCTATCAAGTGCAACAAAAATCGAAAAAGAACAAGAACAAAACAATTGTTTCTTACTGCTCACTAGAATCTAGATAGATGAATTGTGCTGATTCTATCGGTCTAATGACAGGACGTTGGGGGACGTACAATTAAAGTCAATATAACCGAAAAACCTGTGGTAAATACGGTTGATATGTCACTTCTTGAGGCAGAGGAATCCCAATTCATCGACAGTCCCCACAAAGTATATGTAGGAAACATTTCGAGTACTGTCTCTACTGAAACACTCAAAAACTTCTTCTCTGAGAAGGGTAAAGTTCTCAGTGCAAAGGTTTCACGGGTTCCCGGTACATCGAAAGCAAGTGGCTACGGTTTTGTTACCTTTTCTTCCGAGGAGGAAGTCGAAGCTGCCATTTCATCTTTTAACAATGCTGTAAGTAACTCGCTTCCATTCTCTATCATGTCGAACGTTGTAACATCAATACTGATTTATGCAAACTCTATCTCACTTTCTGGTCGGGTTTCATGCATTGGCAACATTTTTGCTCACATTGTCTAGTAGAGCATTCCTTGTTTCATTTTCTGTTTCCCTAAACAATTACATATAGAGTAGCATTCTGATCACCCACTTGTAAGAAGCATTTCTAAACCTAGTCGATTGGTATGCCAACTCGAACAACTTACATTGATCATCGATATTACTAATAGTTGGACAAGCTATGTTCATCAAAGATGATGGCATACTTCCCTGAAAACTCTCATGCACATTTCTCGTTTCCGAACGGGTGGAAGAAGCTTGTGTCTGATATGTGTTTTCCTTTGCTGCAGTTGTTGGAAGGTCAACCAATTCGTGTAAACAAGGCATAATTTTGTAGGATTCTTTGTAACAAAAGGTATGCAAATTTCGCTTTTTACCTCTTGGCATGAATTAATGAACAGCAGAGGTCAGATTTTTCTCTCAATTTCTCTGTATTGATCATTAATGTATAATCTTGTTTATTTTTCTGTTTAAGATTTGACTATTTGCAAATCTTTACTTGTTCCTCAAATTCCACGTTTGACTACTCAACTATTTGCAGTCCTCATGTGCACTATAAATACATCATCTTTTCAAAGGAATTACTCATTTCTCAAATTCTCAGTTCCTCTCAAAACCCAGAAAACAATGATGCTCTCTCTTCTCTCAGCTTTCATAGATTGCTTCCTTCCTTCTCTCTCCCCAAAGGCTTCCCATGGCGGAAGAACAAGTAAACCCCAAACTCAAACAACAGCCTCCAAACTGACACCTGAAGCTGATTCGCAGCTCCCTCAAGCTCCTATAATCGTGTCTCATTTCCCAGTTTCTGCAAACTTATCTTGCTTGTGATTTCCTTGATGAACTCTGGTCTTCAATGGACTCCTCTTGGATCGAATCTGAAAAAGATGAAGTTTTGTCTTTGATCATGTTGTCTCTCGAGTTCAGTCATGGGTGTTTGTAGAACTAGTTTCTTTCTTGTTCCAGCTCTGGTTTTGATTCTTTTTTTCCTTTTTGGAGATTAATATACAACGTTTTTCTTGTAATTTCTTGGTTTCTTCGATGGGTATTGACTTTTCTATCTCATGTTTGTAATGGGTCTTGATTAAAATGTTAAAATACTCCAAATAGCCTTTTATATTTACTACTTTTTTAAGATGGGTCTTTTAGTAGCATAAGGTCAAAGACACTTGAAATAATTGCTCTC

mRNA sequence

GTTCTTGTCGCTTCGGAACGCAAAGCTTTACAGACCAAAAATGGCTTCCATCTCTGCCGTGTTATCGCCAACGAATCTCATCCACAGTTCCACAAAACCCGCCGAAATCCTTCTCAAATCAGTCTCATTTCACCGCCGTCATACCCATACCCTCCTCCGCCTATCTCCCGGTCGCCCCACGAGACTCTTCGCGGCGCTTACTGCTCAGGATTCCAGTTCAGTGGCGGAAAGGAAATTGTACGTTGGAAATATACCTAGAAATGTGGATAATGAGGAGCTCAAGCGGATTGTTGAACAACATGGGGCTGTGGAGAAGGCTGAGGTCATGTATGACAAATACTCTGGAAGAAGCCGCAGGTTTGCCTTTGTTACCATGAAAACGGTTGACGACGCAAATGCAGCAATTGAGAAACTGAATGAGACAGACGTTGGGGGACGTACAATTAAAGTCAATATAACCGAAAAACCTGTGGTAAATACGGTTGATATGTCACTTCTTGAGGCAGAGGAATCCCAATTCATCGACAGTCCCCACAAAGTATATGTAGGAAACATTTCGAGTACTGTCTCTACTGAAACACTCAAAAACTTCTTCTCTGAGAAGGGTAAAGTTCTCAGTGCAAAGGTTTCACGGGTTCCCGGTACATCGAAAGCAAGTGGCTACGGTTTTGTTACCTTTTCTTCCGAGGAGGAAGTCGAAGCTGCCATTTCATCTTTTAACAATGCTTTGTTGGAAGGTCAACCAATTCGTGTAAACAAGGCATAATTTTGTAGGATTCTTTGTAACAAAAGGTATGCAAATTTCGCTTTTTACCTCTTGGCATGAATTAATGAACAGCAGAGGTCAGATTTTTCTCTCAATTTCTCTGTATTGATCATTAATGTATAATCTTGTTTATTTTTCTGTTTAAGATTTGACTATTTGCAAATCTTTACTTGTTCCTCAAATTCCACGTTTGACTACTCAACTATTTGCAGTCCTCATGTGCACTATAAATACATCATCTTTTCAAAGGAATTACTCATTTCTCAAATTCTCAGTTCCTCTCAAAACCCAGAAAACAATGATGCTCTCTCTTCTCTCAGCTTTCATAGATTGCTTCCTTCCTTCTCTCTCCCCAAAGGCTTCCCATGGCGGAAGAACAAGTAAACCCCAAACTCAAACAACAGCCTCCAAACTGACACCTGAAGCTGATTCGCAGCTCCCTCAAGCTCCTATAATCGTGTCTCATTTCCCAGTTTCTGCAAACTTATCTTGCTTGTGATTTCCTTGATGAACTCTGGTCTTCAATGGACTCCTCTTGGATCGAATCTGAAAAAGATGAAGTTTTGTCTTTGATCATGTTGTCTCTCGAGTTCAGTCATGGGTGTTTGTAGAACTAGTTTCTTTCTTGTTCCAGCTCTGGTTTTGATTCTTTTTTTCCTTTTTGGAGATTAATATACAACGTTTTTCTTGTAATTTCTTGGTTTCTTCGATGGGTATTGACTTTTCTATCTCATGTTTGTAATGGGTCTTGATTAAAATGTTAAAATACTCCAAATAGCCTTTTATATTTACTACTTTTTTAAGATGGGTCTTTTAGTAGCATAAGGTCAAAGACACTTGAAATAATTGCTCTC

Coding sequence (CDS)

ATGGCTTCCATCTCTGCCGTGTTATCGCCAACGAATCTCATCCACAGTTCCACAAAACCCGCCGAAATCCTTCTCAAATCAGTCTCATTTCACCGCCGTCATACCCATACCCTCCTCCGCCTATCTCCCGGTCGCCCCACGAGACTCTTCGCGGCGCTTACTGCTCAGGATTCCAGTTCAGTGGCGGAAAGGAAATTGTACGTTGGAAATATACCTAGAAATGTGGATAATGAGGAGCTCAAGCGGATTGTTGAACAACATGGGGCTGTGGAGAAGGCTGAGGTCATGTATGACAAATACTCTGGAAGAAGCCGCAGGTTTGCCTTTGTTACCATGAAAACGGTTGACGACGCAAATGCAGCAATTGAGAAACTGAATGAGACAGACGTTGGGGGACGTACAATTAAAGTCAATATAACCGAAAAACCTGTGGTAAATACGGTTGATATGTCACTTCTTGAGGCAGAGGAATCCCAATTCATCGACAGTCCCCACAAAGTATATGTAGGAAACATTTCGAGTACTGTCTCTACTGAAACACTCAAAAACTTCTTCTCTGAGAAGGGTAAAGTTCTCAGTGCAAAGGTTTCACGGGTTCCCGGTACATCGAAAGCAAGTGGCTACGGTTTTGTTACCTTTTCTTCCGAGGAGGAAGTCGAAGCTGCCATTTCATCTTTTAACAATGCTTTGTTGGAAGGTCAACCAATTCGTGTAAACAAGGCATAA

Protein sequence

MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNKA
Homology
BLAST of Carg13463 vs. NCBI nr
Match: KAG7025672.1 (30S ribosomal protein 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.9 bits (1169), Expect = 4.3e-124
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK
Sbjct: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. NCBI nr
Match: XP_022959724.1 (30S ribosomal protein 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 451.8 bits (1161), Expect = 3.6e-123
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSSTKPAEILLKSVSFHRR THTLLRLSPGRPTRLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRRTHTLLRLSPGRPTRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK
Sbjct: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. NCBI nr
Match: KAG6593325.1 (30S ribosomal protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 450.3 bits (1157), Expect = 1.1e-122
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0

Query: 4   ISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSSVAE 63
           ISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSSVAE
Sbjct: 25  ISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSSVAE 84

Query: 64  RKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANAAIE 123
           RKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANAAIE
Sbjct: 85  RKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANAAIE 144

Query: 124 KLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTETLKN 183
           KLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTETLKN
Sbjct: 145 KLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTETLKN 204

Query: 184 FFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNKA 242
           FFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNKA
Sbjct: 205 FFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNKA 262

BLAST of Carg13463 vs. NCBI nr
Match: XP_023513630.1 (30S ribosomal protein 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 444.5 bits (1142), Expect = 5.8e-121
Identity = 236/241 (97.93%), Postives = 237/241 (98.34%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSS KPAEILL SVSFHRR THT LRLSPGRPTRLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSIKPAEILLTSVSFHRRRTHTFLRLSPGRPTRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEELKRIVE+HGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELKRIVEEHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK
Sbjct: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. NCBI nr
Match: XP_023004641.1 (30S ribosomal protein 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 441.4 bits (1134), Expect = 4.9e-120
Identity = 235/241 (97.51%), Postives = 236/241 (97.93%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSSTKPAEILLKSVSFHRR THTLLRLSPGRP+RLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRRTHTLLRLSPGRPSRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEEL RIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELTRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVN VDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNMVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEK KVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNN LLEGQPIRVNK
Sbjct: 181 LKNFFSEKDKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNVLLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. ExPASy Swiss-Prot
Match: Q8VYM4 (30S ribosomal protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSRP2 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.6e-68
Identity = 129/187 (68.98%), Postives = 161/187 (86.10%), Query Frame = 0

Query: 55  AQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKT 114
           A D SS A R++Y+GNIPR V NE+L ++VE+HGAVEK +VMYDKYSGRSRRF F TMK+
Sbjct: 67  ALDPSSEAARRVYIGNIPRTVTNEQLTKLVEEHGAVEKVQVMYDKYSGRSRRFGFATMKS 126

Query: 115 VDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISS 174
           V+DANA +EKLN   V GR IKVNITEKP+ ++ D+S+L++E+S F+DSP+KVYVGN++ 
Sbjct: 127 VEDANAVVEKLNGNTVEGREIKVNITEKPIASSPDLSVLQSEDSAFVDSPYKVYVGNLAK 186

Query: 175 TVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQ 234
           TV+ E L+N FSEKGKV+SAKVSRVPGTSK++G+GFVTFSSEE+VEAAI + NN+LLEGQ
Sbjct: 187 TVTKEMLENLFSEKGKVVSAKVSRVPGTSKSTGFGFVTFSSEEDVEAAIVALNNSLLEGQ 246

Query: 235 PIRVNKA 242
            IRVNKA
Sbjct: 247 KIRVNKA 253

BLAST of Carg13463 vs. ExPASy Swiss-Prot
Match: P82277 (30S ribosomal protein 2, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSRP2 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-68
Identity = 147/261 (56.32%), Postives = 194/261 (74.33%), Query Frame = 0

Query: 1   MASISAVLSPTNLIH------SSTKP---AEILLKSVSFHRRHTHTL-LRLSPGRPTRLF 60
           MA+IS++L    L+       SS+KP   ++ L++  SF       L  R+S   P    
Sbjct: 1   MATISSILPCHGLLQHCSSSSSSSKPKFSSQNLVQLQSFSNGFGLKLKTRVSTNPPLLKV 60

Query: 61  AALTAQDSSSVA----------ERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKY 120
            A+  +++SS +           R+LYVGNIPRN++N+EL+ IVE+HGA+E AEVMYDKY
Sbjct: 61  RAVVTEETSSSSTASSSSDGEGARRLYVGNIPRNLNNDELRTIVEEHGAIEIAEVMYDKY 120

Query: 121 SGRSRRFAFVTMKTVDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQF 180
           SGRSRRF FVTMKTV+DANA IEKLN+T++GGR IKVNITEKP +  +D++  +AE+SQF
Sbjct: 121 SGRSRRFGFVTMKTVEDANAVIEKLNDTEIGGRKIKVNITEKP-LEGMDIATTQAEDSQF 180

Query: 181 IDSPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVE 240
           ++SP+KVY+GN++ TV+ E LK+FFSEKGKVL AKV R PGTSK++G+GFV+FSSEEEVE
Sbjct: 181 VESPYKVYIGNLAKTVTNELLKDFFSEKGKVLGAKVQRTPGTSKSNGFGFVSFSSEEEVE 240

Query: 241 AAISSFNNALLEGQPIRVNKA 242
           AAI + NN++LEGQ IRVNKA
Sbjct: 241 AAIQALNNSVLEGQKIRVNKA 260

BLAST of Carg13463 vs. ExPASy Swiss-Prot
Match: Q39061 (RNA-binding protein CP33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP33 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-31
Identity = 70/185 (37.84%), Postives = 116/185 (62.70%), Query Frame = 0

Query: 59  SSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDA 118
           +S  E +LYVGN+P  + + EL +I  + G V   +++YDK + RSR F FVTM ++++A
Sbjct: 111 ASGEEGRLYVGNLPYTITSSELSQIFGEAGTVVDVQIVYDKVTDRSRGFGFVTMGSIEEA 170

Query: 119 NAAIEKLNETDVGGRTIKVNITEKP--VVNTVDMSLLEAEESQFIDSPHKVYVGNISSTV 178
             A++  N + +GGRT+KVN  E P    N V  + +      ++DSPHKVY GN+   +
Sbjct: 171 KEAMQMFNSSQIGGRTVKVNFPEVPRGGENEVMRTKIRDNNRSYVDSPHKVYAGNLGWNL 230

Query: 179 STETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPI 238
           +++ LK+ F ++  VL AKV     T ++ G+GF++F S E V++A+++ N   +EG+ +
Sbjct: 231 TSQGLKDAFGDQPGVLGAKVIYERNTGRSRGFGFISFESAENVQSALATMNGVEVEGRAL 290

Query: 239 RVNKA 242
           R+N A
Sbjct: 291 RLNLA 295

BLAST of Carg13463 vs. ExPASy Swiss-Prot
Match: P19684 (33 kDa ribonucleoprotein, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.6e-31
Identity = 73/187 (39.04%), Postives = 119/187 (63.64%), Query Frame = 0

Query: 57  DSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVD 116
           +S SV   +LYVGN+P ++ + +L  I  + G V   E++YD+ + RSR FAFVTM +V+
Sbjct: 107 ESESVEGGRLYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSVE 166

Query: 117 DANAAIEKLNETDVGGRTIKVNITEKP--VVNTVDMSLLEAEESQFIDSPHKVYVGNISS 176
           +A  AI   + + VGGRT+KVN  E P      V  + + +    F+DSPHK+YV N+S 
Sbjct: 167 EAKEAIRLFDGSQVGGRTVKVNFPEVPRGGEREVMSAKIRSTYQGFVDSPHKLYVANLSW 226

Query: 177 TVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQ 236
            ++++ L++ F+++   +SAKV     + ++ G+GF+TFSS E + +A+ + N   LEG+
Sbjct: 227 ALTSQGLRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMNSALDTMNEVELEGR 286

Query: 237 PIRVNKA 242
           P+R+N A
Sbjct: 287 PLRLNVA 293

BLAST of Carg13463 vs. ExPASy Swiss-Prot
Match: Q9ZUU4 (RNA-binding protein CP29B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP29B PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.6e-28
Identity = 77/200 (38.50%), Postives = 116/200 (58.00%), Query Frame = 0

Query: 56  QDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTV 115
           ++ S  A+ KL+VGN+P NVD+ +L ++ E  G VE  EV+YDK +GRSR F FVTM +V
Sbjct: 83  KEQSFSADLKLFVGNLPFNVDSAQLAQLFESAGNVEMVEVIYDKITGRSRGFGFVTMSSV 142

Query: 116 DDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQF--------------I 175
            +  AA ++ N  ++ GR ++VN    P       S      S F               
Sbjct: 143 SEVEAAAQQFNGYELDGRPLRVNAGPPPPKREDGFS--RGPRSSFGSSGSGYGGGGGSGA 202

Query: 176 DSPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEA 235
            S ++VYVGN+S  V    L++ FSE+GKV+ A+V     + ++ G+GFVT+ S +EV+ 
Sbjct: 203 GSGNRVYVGNLSWGVDDMALESLFSEQGKVVEARVIYDRDSGRSKGFGFVTYDSSQEVQN 262

Query: 236 AISSFNNALLEGQPIRVNKA 242
           AI S + A L+G+ IRV++A
Sbjct: 263 AIKSLDGADLDGRQIRVSEA 280

BLAST of Carg13463 vs. ExPASy TrEMBL
Match: A0A6J1H8X7 (30S ribosomal protein 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460708 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.8e-123
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSSTKPAEILLKSVSFHRR THTLLRLSPGRPTRLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRRTHTLLRLSPGRPTRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK
Sbjct: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. ExPASy TrEMBL
Match: A0A6J1KSN9 (30S ribosomal protein 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497873 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.4e-120
Identity = 235/241 (97.51%), Postives = 236/241 (97.93%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSS 60
           MASISAVLSPTNLIHSSTKPAEILLKSVSFHRR THTLLRLSPGRP+RLFAALTAQDSSS
Sbjct: 1   MASISAVLSPTNLIHSSTKPAEILLKSVSFHRRRTHTLLRLSPGRPSRLFAALTAQDSSS 60

Query: 61  VAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120
           VAERKLYVGNIPRNVDNEEL RIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA
Sbjct: 61  VAERKLYVGNIPRNVDNEELTRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANA 120

Query: 121 AIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180
           AIEKLNETDVGGRTIKVNITEKPVVN VDMSLLEAEESQFIDSPHKVYVGNISSTVSTET
Sbjct: 121 AIEKLNETDVGGRTIKVNITEKPVVNMVDMSLLEAEESQFIDSPHKVYVGNISSTVSTET 180

Query: 181 LKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNK 240
           LKNFFSEK KVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNN LLEGQPIRVNK
Sbjct: 181 LKNFFSEKDKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNVLLEGQPIRVNK 240

Query: 241 A 242
           A
Sbjct: 241 A 241

BLAST of Carg13463 vs. ExPASy TrEMBL
Match: A0A6J1JXN0 (30S ribosomal protein 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488418 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.4e-101
Identity = 208/259 (80.31%), Postives = 226/259 (87.26%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEI-------LLKSVSFHRRHTHTLLRLSPG-------RP 60
           MASIS++L+P NLIHSS KP EI        L+ +SFH R  +TLLR+SPG       R 
Sbjct: 1   MASISSILTPANLIHSSIKPTEIPQNLSTQFLRPISFHPR--YTLLRVSPGLQTSAGKRR 60

Query: 61  TRLF----AALTAQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSG 120
           TRLF    AA++ QD SSVA RKLY+GNIPRNV NEEL RIVE+HGAVEKAEVMYDKYSG
Sbjct: 61  TRLFAVAEAAVSVQDPSSVAARKLYIGNIPRNVGNEELTRIVEEHGAVEKAEVMYDKYSG 120

Query: 121 RSRRFAFVTMKTVDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFID 180
           RSRRFAFVTMKTVDDANA IEKLN+T+VGGRTIKVNITEKP+VNTVDMSLL+A+ESQFID
Sbjct: 121 RSRRFAFVTMKTVDDANAVIEKLNDTEVGGRTIKVNITEKPLVNTVDMSLLQADESQFID 180

Query: 181 SPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAA 240
           SP+KVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSK+SGYGFVTFSSEEEVEAA
Sbjct: 181 SPYKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKSSGYGFVTFSSEEEVEAA 240

Query: 241 ISSFNNALLEGQPIRVNKA 242
           ISSFNNALLEGQPIRVNKA
Sbjct: 241 ISSFNNALLEGQPIRVNKA 257

BLAST of Carg13463 vs. ExPASy TrEMBL
Match: A0A6J1GSN8 (30S ribosomal protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456607 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.4e-101
Identity = 208/259 (80.31%), Postives = 226/259 (87.26%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEI-------LLKSVSFHRRHTHTLLRLSPG-------RP 60
           MASIS++L+P NLIHSS KP EI        L+ +SFH R  +TLLR+SPG       R 
Sbjct: 1   MASISSILTPANLIHSSIKPTEIPQTLSTQFLRPISFHPR--YTLLRVSPGLQTSAGKRR 60

Query: 61  TRLF----AALTAQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSG 120
           TRLF    AA++ QD SSVA RKLY+GNIPRNV NEEL RIVE+HGAVEKAEVMYDKYSG
Sbjct: 61  TRLFAVAEAAVSVQDPSSVAARKLYIGNIPRNVGNEELTRIVEEHGAVEKAEVMYDKYSG 120

Query: 121 RSRRFAFVTMKTVDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFID 180
           RSRRFAFVTMKTVDDANA IEKLN+T+VGGRTIKVNITEKP+VNTVDMSLL+A+ESQFID
Sbjct: 121 RSRRFAFVTMKTVDDANAVIEKLNDTEVGGRTIKVNITEKPLVNTVDMSLLQADESQFID 180

Query: 181 SPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAA 240
           SP+KVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSK+SGYGFVTFSSEEEVEAA
Sbjct: 181 SPYKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKSSGYGFVTFSSEEEVEAA 240

Query: 241 ISSFNNALLEGQPIRVNKA 242
           ISSFNNALLEGQPIRVNKA
Sbjct: 241 ISSFNNALLEGQPIRVNKA 257

BLAST of Carg13463 vs. ExPASy TrEMBL
Match: A0A6J1GQT6 (30S ribosomal protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456607 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.4e-101
Identity = 208/259 (80.31%), Postives = 226/259 (87.26%), Query Frame = 0

Query: 1   MASISAVLSPTNLIHSSTKPAEI-------LLKSVSFHRRHTHTLLRLSPG-------RP 60
           MASIS++L+P NLIHSS KP EI        L+ +SFH R  +TLLR+SPG       R 
Sbjct: 23  MASISSILTPANLIHSSIKPTEIPQTLSTQFLRPISFHPR--YTLLRVSPGLQTSAGKRR 82

Query: 61  TRLF----AALTAQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSG 120
           TRLF    AA++ QD SSVA RKLY+GNIPRNV NEEL RIVE+HGAVEKAEVMYDKYSG
Sbjct: 83  TRLFAVAEAAVSVQDPSSVAARKLYIGNIPRNVGNEELTRIVEEHGAVEKAEVMYDKYSG 142

Query: 121 RSRRFAFVTMKTVDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFID 180
           RSRRFAFVTMKTVDDANA IEKLN+T+VGGRTIKVNITEKP+VNTVDMSLL+A+ESQFID
Sbjct: 143 RSRRFAFVTMKTVDDANAVIEKLNDTEVGGRTIKVNITEKPLVNTVDMSLLQADESQFID 202

Query: 181 SPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAA 240
           SP+KVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSK+SGYGFVTFSSEEEVEAA
Sbjct: 203 SPYKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKSSGYGFVTFSSEEEVEAA 262

Query: 241 ISSFNNALLEGQPIRVNKA 242
           ISSFNNALLEGQPIRVNKA
Sbjct: 263 ISSFNNALLEGQPIRVNKA 279

BLAST of Carg13463 vs. TAIR 10
Match: AT3G52150.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-69
Identity = 129/187 (68.98%), Postives = 161/187 (86.10%), Query Frame = 0

Query: 55  AQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKT 114
           A D SS A R++Y+GNIPR V NE+L ++VE+HGAVEK +VMYDKYSGRSRRF F TMK+
Sbjct: 67  ALDPSSEAARRVYIGNIPRTVTNEQLTKLVEEHGAVEKVQVMYDKYSGRSRRFGFATMKS 126

Query: 115 VDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISS 174
           V+DANA +EKLN   V GR IKVNITEKP+ ++ D+S+L++E+S F+DSP+KVYVGN++ 
Sbjct: 127 VEDANAVVEKLNGNTVEGREIKVNITEKPIASSPDLSVLQSEDSAFVDSPYKVYVGNLAK 186

Query: 175 TVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQ 234
           TV+ E L+N FSEKGKV+SAKVSRVPGTSK++G+GFVTFSSEE+VEAAI + NN+LLEGQ
Sbjct: 187 TVTKEMLENLFSEKGKVVSAKVSRVPGTSKSTGFGFVTFSSEEDVEAAIVALNNSLLEGQ 246

Query: 235 PIRVNKA 242
            IRVNKA
Sbjct: 247 KIRVNKA 253

BLAST of Carg13463 vs. TAIR 10
Match: AT3G52150.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-69
Identity = 129/187 (68.98%), Postives = 161/187 (86.10%), Query Frame = 0

Query: 55  AQDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKT 114
           A D SS A R++Y+GNIPR V NE+L ++VE+HGAVEK +VMYDKYSGRSRRF F TMK+
Sbjct: 67  ALDPSSEAARRVYIGNIPRTVTNEQLTKLVEEHGAVEKVQVMYDKYSGRSRRFGFATMKS 126

Query: 115 VDDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISS 174
           V+DANA +EKLN   V GR IKVNITEKP+ ++ D+S+L++E+S F+DSP+KVYVGN++ 
Sbjct: 127 VEDANAVVEKLNGNTVEGREIKVNITEKPIASSPDLSVLQSEDSAFVDSPYKVYVGNLAK 186

Query: 175 TVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQ 234
           TV+ E L+N FSEKGKV+SAKVSRVPGTSK++G+GFVTFSSEE+VEAAI + NN+LLEGQ
Sbjct: 187 TVTKEMLENLFSEKGKVVSAKVSRVPGTSKSTGFGFVTFSSEEDVEAAIVALNNSLLEGQ 246

Query: 235 PIRVNKA 242
            IRVNKA
Sbjct: 247 KIRVNKA 253

BLAST of Carg13463 vs. TAIR 10
Match: AT3G52380.1 (chloroplast RNA-binding protein 33 )

HSP 1 Score: 137.9 bits (346), Expect = 1.1e-32
Identity = 70/185 (37.84%), Postives = 116/185 (62.70%), Query Frame = 0

Query: 59  SSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDA 118
           +S  E +LYVGN+P  + + EL +I  + G V   +++YDK + RSR F FVTM ++++A
Sbjct: 111 ASGEEGRLYVGNLPYTITSSELSQIFGEAGTVVDVQIVYDKVTDRSRGFGFVTMGSIEEA 170

Query: 119 NAAIEKLNETDVGGRTIKVNITEKP--VVNTVDMSLLEAEESQFIDSPHKVYVGNISSTV 178
             A++  N + +GGRT+KVN  E P    N V  + +      ++DSPHKVY GN+   +
Sbjct: 171 KEAMQMFNSSQIGGRTVKVNFPEVPRGGENEVMRTKIRDNNRSYVDSPHKVYAGNLGWNL 230

Query: 179 STETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPI 238
           +++ LK+ F ++  VL AKV     T ++ G+GF++F S E V++A+++ N   +EG+ +
Sbjct: 231 TSQGLKDAFGDQPGVLGAKVIYERNTGRSRGFGFISFESAENVQSALATMNGVEVEGRAL 290

Query: 239 RVNKA 242
           R+N A
Sbjct: 291 RLNLA 295

BLAST of Carg13463 vs. TAIR 10
Match: AT1G60000.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-31
Identity = 84/226 (37.17%), Postives = 126/226 (55.75%), Query Frame = 0

Query: 16  SSTKPAEILLKSVSFHRRHTHTLLRLSPGRPTRLFAALTAQDSSSVAERKLYVGNIPRNV 75
           S+ KP  I  +S S   R     + +      +   A    D  +    KLY GN+P NV
Sbjct: 37  STPKPLLISSRSCSRRFRVLSETITVKLEEEEKDDGASAVLDPPAAVNTKLYFGNLPYNV 96

Query: 76  DNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTVDDANAAIEKLNETDVGGRTI 135
           D+  L +I++     E  EV+Y++ +G+SR FAFVTM  V+D N  I+ L+ T+  GR +
Sbjct: 97  DSATLAQIIQDFANPELVEVLYNRDTGQSRGFAFVTMSNVEDCNIIIDNLDGTEYLGRAL 156

Query: 136 KVNITEKPVVNTVDMSLLEAEESQFIDSPHKVYVGNISSTVSTETLKNFFSEKGKVLSAK 195
           KVN  +KP  N         +E  + ++ HK++VGN+S TV++E+L   F E G V+ A+
Sbjct: 157 KVNFADKPKPN---------KEPLYPETEHKLFVGNLSWTVTSESLAGAFRECGDVVGAR 216

Query: 196 VSRVPGTSKASGYGFVTFSSEEEVEAAISSFNNALLEGQPIRVNKA 242
           V     T ++ GYGFV +SS+ E+E A+ S +   LEG+ IRVN A
Sbjct: 217 VVFDGDTGRSRGYGFVCYSSKAEMETALESLDGFELEGRAIRVNLA 253

BLAST of Carg13463 vs. TAIR 10
Match: AT2G37220.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 126.3 bits (316), Expect = 3.3e-29
Identity = 77/200 (38.50%), Postives = 116/200 (58.00%), Query Frame = 0

Query: 56  QDSSSVAERKLYVGNIPRNVDNEELKRIVEQHGAVEKAEVMYDKYSGRSRRFAFVTMKTV 115
           ++ S  A+ KL+VGN+P NVD+ +L ++ E  G VE  EV+YDK +GRSR F FVTM +V
Sbjct: 83  KEQSFSADLKLFVGNLPFNVDSAQLAQLFESAGNVEMVEVIYDKITGRSRGFGFVTMSSV 142

Query: 116 DDANAAIEKLNETDVGGRTIKVNITEKPVVNTVDMSLLEAEESQF--------------I 175
            +  AA ++ N  ++ GR ++VN    P       S      S F               
Sbjct: 143 SEVEAAAQQFNGYELDGRPLRVNAGPPPPKREDGFS--RGPRSSFGSSGSGYGGGGGSGA 202

Query: 176 DSPHKVYVGNISSTVSTETLKNFFSEKGKVLSAKVSRVPGTSKASGYGFVTFSSEEEVEA 235
            S ++VYVGN+S  V    L++ FSE+GKV+ A+V     + ++ G+GFVT+ S +EV+ 
Sbjct: 203 GSGNRVYVGNLSWGVDDMALESLFSEQGKVVEARVIYDRDSGRSKGFGFVTYDSSQEVQN 262

Query: 236 AISSFNNALLEGQPIRVNKA 242
           AI S + A L+G+ IRV++A
Sbjct: 263 AIKSLDGADLDGRQIRVSEA 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025672.14.3e-124100.0030S ribosomal protein 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022959724.13.6e-12399.5930S ribosomal protein 2, chloroplastic-like [Cucurbita moschata][more]
KAG6593325.11.1e-122100.0030S ribosomal protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023513630.15.8e-12197.9330S ribosomal protein 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023004641.14.9e-12097.5130S ribosomal protein 2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VYM41.6e-6868.9830S ribosomal protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSRP2 ... [more]
P822773.5e-6856.3230S ribosomal protein 2, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSRP2 PE=... [more]
Q390611.5e-3137.84RNA-binding protein CP33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP33 ... [more]
P196842.6e-3139.0433 kDa ribonucleoprotein, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=1 SV=... [more]
Q9ZUU44.6e-2838.50RNA-binding protein CP29B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP29... [more]
Match NameE-valueIdentityDescription
A0A6J1H8X71.8e-12399.5930S ribosomal protein 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KSN92.4e-12097.5130S ribosomal protein 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1JXN09.4e-10180.3130S ribosomal protein 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GSN89.4e-10180.3130S ribosomal protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1GQT69.4e-10180.3130S ribosomal protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT3G52150.11.1e-6968.98RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT3G52150.21.1e-6968.98RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT3G52380.11.1e-3237.84chloroplast RNA-binding protein 33 [more]
AT1G60000.11.2e-3137.17RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT2G37220.13.3e-2938.50RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 166..239
e-value: 5.9E-22
score: 88.9
coord: 65..138
e-value: 2.5E-27
score: 106.8
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 167..237
e-value: 6.0E-17
score: 61.3
coord: 66..136
e-value: 1.7E-20
score: 72.6
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 165..241
score: 18.188618
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 64..142
score: 18.700279
IPR003954RNA recognition motif domain, eukaryoteSMARTSM00361rrm2_1coord: 166..239
e-value: 0.11
score: 21.6
coord: 65..138
e-value: 0.8
score: 15.2
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 152..241
e-value: 3.1E-23
score: 84.1
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 44..147
e-value: 1.6E-27
score: 98.4
NoneNo IPR availablePANTHERPTHR48025OS02G0815200 PROTEINcoord: 14..241
NoneNo IPR availablePANTHERPTHR48025:SF430S RIBOSOMAL PROTEIN 2, CHLOROPLASTICcoord: 14..241
NoneNo IPR availableCDDcd12399RRM_HP0827_likecoord: 65..142
e-value: 1.34228E-35
score: 119.64
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 55..142
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 160..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13463-RACarg13463-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005840 ribosome
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding