Carg13458 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13458
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AAI domain-containing protein
Location	Carg_Chr08: 2936563 .. 2937130 (-)
RNA-Seq Expression	Carg13458
Synteny	Carg13458

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCCGGGAAGCTTCAAACCTCTGCAATTTCTAGTGTTCATGATAGCAATGACATGCTTTGTGGAGCCAAGCAAAGCCAAGTTCTGTTCAAACAGTTTGATTTCCACGTTGGTTCAGCTCATTCCTTGCAAGCCATCACTGTCGCCATTCAGCCCCATGCCACCAAGTCTCCTCTGTTGCCATGCGCTCAAGACTCTCGGCCAGCCATGTCTCTGTGCAGTGTTGGACGCCCCTCCTGTTTCTGGTATTGATTACAACGTGGCCATGTTGCTTCCTCAGAAATGCGCTTTGAATTTCCACCCATGTAACTCTTTCTTCTTCTTCAATCTCTCAACATTTTTTCTGGTTGTTTTGTGTTGAAGACTCTTCAAGAAACTTGATTTTGTTCTTGTTCTTGTTGTTCTTATGCAGGTTTTTAGAAGATGGAGGATGGTGAGATGTTTGAAGATGATGAATAAAACTGCTTGTTATGTTTTGATATCATGTGGTTGGATATATGTTTTATGAAAAAATAAATGTTTGTTTCAACTTTCCAATCGAAATTTATAGAAAGTCGAGTTTGATC

mRNA sequence

ATGAAGCCGGGAAGCTTCAAACCTCTGCAATTTCTAGTGTTCATGATAGCAATGACATGCTTTGTGGAGCCAAGCAAAGCCAAGTTCTGTTCAAACAGTTTGATTTCCACGTTGGTTCAGCTCATTCCTTGCAAGCCATCACTGTCGCCATTCAGCCCCATGCCACCAAGTCTCCTCTGTTGCCATGCGCTCAAGACTCTCGGCCAGCCATGTCTCTGTGCAGTGTTGGACGCCCCTCCTGTTTCTGGTATTGATTACAACGTGGCCATGTTGCTTCCTCAGAAATGCGCTTTGAATTTCCACCCATGTAACTCTTTCTTCTTCTTCAATCTCTCAACATTTTTTCTGGTTTTTAGAAGATGGAGGATGGTGAGATGTTTGAAGATGATGAATAAAACTGCTTGTTATGTTTTGATATCATGTGGTTGGATATATGTTTTATGAAAAAATAAATGTTTGTTTCAACTTTCCAATCGAAATTTATAGAAAGTCGAGTTTGATC

Coding sequence (CDS)

ATGAAGCCGGGAAGCTTCAAACCTCTGCAATTTCTAGTGTTCATGATAGCAATGACATGCTTTGTGGAGCCAAGCAAAGCCAAGTTCTGTTCAAACAGTTTGATTTCCACGTTGGTTCAGCTCATTCCTTGCAAGCCATCACTGTCGCCATTCAGCCCCATGCCACCAAGTCTCCTCTGTTGCCATGCGCTCAAGACTCTCGGCCAGCCATGTCTCTGTGCAGTGTTGGACGCCCCTCCTGTTTCTGGTATTGATTACAACGTGGCCATGTTGCTTCCTCAGAAATGCGCTTTGAATTTCCACCCATGTAACTCTTTCTTCTTCTTCAATCTCTCAACATTTTTTCTGGTTTTTAGAAGATGGAGGATGGTGAGATGTTTGAAGATGATGAATAAAACTGCTTGTTATGTTTTGATATCATGTGGTTGGATATATGTTTTATGA

Protein sequence

MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLCCHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCNSFFFFNLSTFFLVFRRWRMVRCLKMMNKTACYVLISCGWIYVL

Homology

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BLAST of Carg13458 vs. NCBI nr
Match: KAG7025667.1 (hypothetical protein SDJN02_12165, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 307.4 bits (786), Expect = 6.8e-80
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC
Sbjct: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCNSFFFFNLSTFFLVFRR 120
           CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCNSFFFFNLSTFFLVFRR
Sbjct: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCNSFFFFNLSTFFLVFRR 120

Query: 121 WRMVRCLKMMNKTACYVLISCGWIYVL 148
           WRMVRCLKMMNKTACYVLISCGWIYVL
Sbjct: 121 WRMVRCLKMMNKTACYVLISCGWIYVL 147

[Close]

BLAST of Carg13458 vs. NCBI nr
Match: XP_023004704.1 (putative lipid-transfer protein DIR1 [Cucurbita maxima] >KAG6593320.1 hypothetical protein SDJN03_12796, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 217.6 bits (553), Expect = 7.0e-53
Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC
Sbjct: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC
Sbjct: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 103

[Close]

BLAST of Carg13458 vs. NCBI nr
Match: XP_023514059.1 (putative lipid-transfer protein DIR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.5 bits (545), Expect = 6.0e-52
Identity = 102/103 (99.03%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MKPGSFKPLQFLVFMIAMTCFVE SKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC
Sbjct: 1   MKPGSFKPLQFLVFMIAMTCFVEQSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC
Sbjct: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 103

[Close]

BLAST of Carg13458 vs. NCBI nr
Match: XP_038900004.1 (non-specific lipid-transfer protein P5-like [Benincasa hispida])

HSP 1 Score: 175.6 bits (444), Expect = 3.1e-40
Identity = 79/103 (76.70%), Postives = 93/103 (90.29%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK  +FKPLQFLVFM+A+ CF+E  KAKFCSNSLIS+LVQLIPC+P+LSPF P+PPS+LC
Sbjct: 1   MKQANFKPLQFLVFMMAIACFLEQGKAKFCSNSLISSLVQLIPCRPALSPFHPIPPSILC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           C+A+KTLGQ CLCA+LDAPPVSG+DYN+AM LPQKCA NF PC
Sbjct: 61  CNAIKTLGQSCLCALLDAPPVSGVDYNLAMSLPQKCATNFDPC 103

[Close]

BLAST of Carg13458 vs. NCBI nr
Match: KGN45155.1 (hypothetical protein Csa_016316 [Cucumis sativus])

HSP 1 Score: 166.8 bits (421), Expect = 1.4e-37
Identity = 76/103 (73.79%), Postives = 90/103 (87.38%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK   FKPL+FLVFM+A+T F+  +KAKFCSNSLI +LVQLIPC+PSLSPF P+PPSL+C
Sbjct: 1   MKQAIFKPLEFLVFMLAITSFLGQAKAKFCSNSLIYSLVQLIPCRPSLSPFHPIPPSLVC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           C A+KTLGQ C+CA+LDAPPVSG+DYN+AM LPQKCA NF PC
Sbjct: 61  CDAIKTLGQSCICALLDAPPVSGVDYNLAMSLPQKCAANFEPC 103

[Close]

BLAST of Carg13458 vs. ExPASy TrEMBL
Match: A0A6J1KX32 (putative lipid-transfer protein DIR1 OS=Cucurbita maxima OX=3661 GN=LOC111497920 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.4e-53
Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC
Sbjct: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC
Sbjct: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 103

[Close]

BLAST of Carg13458 vs. ExPASy TrEMBL
Match: A0A0A0K635 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428920 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 6.9e-38
Identity = 76/103 (73.79%), Postives = 90/103 (87.38%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK   FKPL+FLVFM+A+T F+  +KAKFCSNSLI +LVQLIPC+PSLSPF P+PPSL+C
Sbjct: 1   MKQAIFKPLEFLVFMLAITSFLGQAKAKFCSNSLIYSLVQLIPCRPSLSPFHPIPPSLVC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           C A+KTLGQ C+CA+LDAPPVSG+DYN+AM LPQKCA NF PC
Sbjct: 61  CDAIKTLGQSCICALLDAPPVSGVDYNLAMSLPQKCAANFEPC 103

[Close]

BLAST of Carg13458 vs. ExPASy TrEMBL
Match: A0A1S3CEQ8 (protein LIM1-like OS=Cucumis melo OX=3656 GN=LOC103499902 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.1e-35
Identity = 72/103 (69.90%), Postives = 88/103 (85.44%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK   FKPL+FLVF++A+  F+   KAKFCSNSLI +LVQL+PC+PSLSPF P+PPS LC
Sbjct: 1   MKQAIFKPLEFLVFILAIASFLGQGKAKFCSNSLIYSLVQLLPCRPSLSPFHPIPPSPLC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           C+A++TLGQ C+CA+LDAPPVSG+DYN+AM LPQKCA NF PC
Sbjct: 61  CNAIETLGQSCICALLDAPPVSGVDYNLAMSLPQKCASNFEPC 103

[Close]

BLAST of Carg13458 vs. ExPASy TrEMBL
Match: V4SWN4 (AAI domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10033078mg PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.5e-27
Identity = 57/104 (54.81%), Postives = 77/104 (74.04%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK    K L  L+ ++ MT F+E   +  C+N+ ISTLVQLIPC+P++SPFSP+PPS  C
Sbjct: 1   MKLSGTKALPILLLVLVMTSFMEQGNSHPCANTFISTLVQLIPCRPAVSPFSPIPPSEAC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCN 105
           C+A+K LGQPCLC +++ PP++GID NVA+ LP KC  NF PC+
Sbjct: 61  CNAIKALGQPCLCVLINGPPIAGIDRNVALQLPDKCIANFEPCD 104

[Close]

BLAST of Carg13458 vs. ExPASy TrEMBL
Match: A0A067H6Y1 (AAI domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g039389mg PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 9.4e-27
Identity = 57/103 (55.34%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MKPGSFKPLQFLVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLC 60
           MK    K L  L+ ++ MT F+E   +  C+N+ ISTLVQLIPC+P++SPFSP+PPS  C
Sbjct: 1   MKLSGTKALPILLLVLVMTSFMEQGNSHPCANTFISTLVQLIPCRPAVSPFSPIPPSEAC 60

Query: 61  CHALKTLGQPCLCAVLDAPPVSGIDYNVAMLLPQKCALNFHPC 104
           C+A+K LGQPCLC +++ PP++GID NVA+ LP KC  NF PC
Sbjct: 61  CNAIKALGQPCLCVLINGPPIAGIDRNVALQLPDKCIANFEPC 103

[Close]

BLAST of Carg13458 vs. TAIR 10
Match: AT3G07450.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 2.4e-22
Identity = 43/93 (46.24%), Postives = 64/93 (68.82%), Query Frame = 0


Query: 12  LVFMIAMTCFVEPSKAKFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLCCHALKTLGQPC 71
           L+ ++  +  V  +    C  + +S L+QL+PC+PS++PFS +PP+ LCC A+KTLGQPC
Sbjct: 14  LLMVMVQSGLVRETLGHPCGRTFLSALIQLVPCRPSVAPFSTLPPNGLCCAAIKTLGQPC 73

Query: 72  LCAVLDAPPVSGIDYNVAMLLPQKCALNFHPCN 105
           LC +   PP+ G+D  +A+ LP KC+ NF PCN
Sbjct: 74  LCVLAKGPPIVGVDRTLALHLPGKCSANFLPCN 106

[Close]

BLAST of Carg13458 vs. TAIR 10
Match: AT3G52130.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 8.9e-22
Identity = 41/75 (54.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 30  CSNSLISTLVQLIPCKPSLSPFSPMPPSLLCCHALKTLGQPCLCAVLDAPPVSGIDYNVA 89
           CS +  S LVQLIPC+ +++PFSP+PP+ +CC A+ TLG+PCLC + + PP+SGID ++A
Sbjct: 48  CSRTFFSALVQLIPCRAAVAPFSPIPPTEICCSAVVTLGRPCLCLLANGPPLSGIDRSMA 107

Query: 90  MLLPQKCALNFHPCN 105
           + LPQ+C+ NF PC+
Sbjct: 108 LQLPQRCSANFPPCD 122

[Close]

BLAST of Carg13458 vs. TAIR 10
Match: AT5G48490.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.4e-06
Identity = 33/94 (35.11%), Postives = 49/94 (52.13%), Query Frame = 0


Query: 12  LVFMIAMTCFVEPSKA-KFCSNSLISTLVQLIPCKPSLSPFSPMPPSLLCCHALKTLGQP 71
           ++ M+  +  VE S A   C      T  +L  C P++S  +P  PSLLCC+ALK     
Sbjct: 10  VIVMVMASLVVERSVAIDLCG----MTQAELNECLPAVSKNNPTSPSLLCCNALKHADYT 69

Query: 72  CLCAVLDAPPVS--GIDYNVAMLLPQKCALNFHP 103
           CLC   ++P +   G+D  +A  LP++C L   P
Sbjct: 70  CLCGYKNSPWLGSFGVDPKLASSLPKECDLTNAP 99

The following BLAST results are available for this feature:

BLAST of Carg13458 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025667.1	6.8e-80	100.00	hypothetical protein SDJN02_12165, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023004704.1	7.0e-53	100.00	putative lipid-transfer protein DIR1 [Cucurbita maxima] >KAG6593320.1 hypothetic...	[more]
XP_023514059.1	6.0e-52	99.03	putative lipid-transfer protein DIR1 [Cucurbita pepo subsp. pepo]	[more]
XP_038900004.1	3.1e-40	76.70	non-specific lipid-transfer protein P5-like [Benincasa hispida]	[more]
KGN45155.1	1.4e-37	73.79	hypothetical protein Csa_016316 [Cucumis sativus]	[more]

BLAST of Carg13458 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg13458 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KX32	3.4e-53	100.00	putative lipid-transfer protein DIR1 OS=Cucurbita maxima OX=3661 GN=LOC111497920...	[more]
A0A0A0K635	6.9e-38	73.79	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428920 PE=4 SV...	[more]
A0A1S3CEQ8	1.1e-35	69.90	protein LIM1-like OS=Cucumis melo OX=3656 GN=LOC103499902 PE=4 SV=1	[more]
V4SWN4	5.5e-27	54.81	AAI domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10033078mg...	[more]
A0A067H6Y1	9.4e-27	55.34	AAI domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g039389mg PE=...	[more]

BLAST of Carg13458 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07450.1	2.4e-22	46.24	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G52130.1	8.9e-22	54.67	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT5G48490.1	2.4e-06	35.11	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 13..101 e-value: 8.8E-8 score: 32.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 28..103 e-value: 1.6E-10 score: 42.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 37..105
IPR039265	Putative lipid-transfer protein DIR1-like	PANTHER	PTHR33122	LIPID BINDING PROTEIN-RELATED	coord: 2..104
None	No IPR available	PANTHER	PTHR33122:SF33	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 2..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13458-RA	Carg13458-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009627	systemic acquired resistance
molecular_function	GO:0005504	fatty acid binding