Carg13402 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13402
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein NIM1-INTERACTING 1
Location	Carg_Chr08: 2587900 .. 2588289 (-)
RNA-Seq Expression	Carg13402
Synteny	Carg13402

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCAACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

mRNA sequence

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCAACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

Coding sequence (CDS)

ATGGAAAAGCAGAGCTTGAAGACGACGGGGAAGAGAGTGGCTTCCGCCGCCGTCGACGGAGAGGAGGAGGAGGAGGAGGAGATGAAGATGGAGGCGTTCTTTGCTCTGGTGAGGAATTTCAAGGAAATGCGAGATCAGCGGAGGAAGGAGTTGATGATCGGCTGCAACGGCGAGAAAGCGGCAGCGGCGGAGATGAGGAAGAGGAAGATGAAGACGGTGGAGAATCGGTCGGCGTGGATTCCGAGGTTTGAAAGAGAGGATTTCGATGAGGAGTTTCAGGTGAGAGGTCCGGCGAAGGAAGAGGCGGCGGCGGCGGCGATCGCGACGGCGAAGAAGAAGAAGAAAGTAAAGAACGAAGAGGATATTTTGGATCTTAATCTGTCTCTGTAG

Protein sequence

MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEEDILDLNLSL

Homology

[Close]

BLAST of Carg13402 vs. NCBI nr
Match: KAG7025612.1 (hypothetical protein SDJN02_12109, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 238.4 bits (607), Expect = 3.4e-59
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60
           MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA
Sbjct: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60

Query: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120
           AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE
Sbjct: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120

Query: 121 DILDLNLSL 130
           DILDLNLSL
Sbjct: 121 DILDLNLSL 129

[Close]

BLAST of Carg13402 vs. NCBI nr
Match: KAG6593261.1 (hypothetical protein SDJN03_12737, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.8 bits (582), Expect = 2.7e-56
Identity = 126/129 (97.67%), Postives = 126/129 (97.67%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60
           MEKQSLKTTGKRVASAAVDG  EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA
Sbjct: 1   MEKQSLKTTGKRVASAAVDG--EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60

Query: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120
           AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAI TAKKKKKVKNEE
Sbjct: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIVTAKKKKKVKNEE 120

Query: 121 DILDLNLSL 130
           DILDLNLSL
Sbjct: 121 DILDLNLSL 127

[Close]

BLAST of Carg13402 vs. NCBI nr
Match: XP_022960173.1 (protein NIM1-INTERACTING 1 [Cucurbita moschata])

HSP 1 Score: 222.6 bits (566), Expect = 1.9e-54
Identity = 126/133 (94.74%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVAS----AAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCN 60
           MEKQSLKTTGKRVAS    AAVDG  EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC+
Sbjct: 1   MEKQSLKTTGKRVASAAAAAAVDG--EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCD 60

Query: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120
           GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV
Sbjct: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120

Query: 121 KNEEDILDLNLSL 130
           KNEEDILDLNLSL
Sbjct: 121 KNEEDILDLNLSL 131

[Close]

BLAST of Carg13402 vs. NCBI nr
Match: XP_023514605.1 (protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 212.2 bits (539), Expect = 2.6e-51
Identity = 120/129 (93.02%), Postives = 122/129 (94.57%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60
           MEKQSLK TGKRVA AAVDG EEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC+GEKA
Sbjct: 1   MEKQSLKPTGKRVAVAAVDG-EEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCDGEKA 60

Query: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120
           AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQ+RGPAKEEAAAA    AKKKKKVKNEE
Sbjct: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQLRGPAKEEAAAA----AKKKKKVKNEE 120

Query: 121 DILDLNLSL 130
           DILDLNLSL
Sbjct: 121 DILDLNLSL 124

[Close]

BLAST of Carg13402 vs. NCBI nr
Match: XP_023004697.1 (protein NIM1-INTERACTING 1 [Cucurbita maxima])

HSP 1 Score: 206.1 bits (523), Expect = 1.9e-49
Identity = 115/129 (89.15%), Postives = 119/129 (92.25%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60
           MEKQSLKTTGKRVA+A VD EEE+EEEMKMEAFF LVRNFKEMRDQRRKELMI C+GEKA
Sbjct: 1   MEKQSLKTTGKRVAAATVDREEEKEEEMKMEAFFDLVRNFKEMRDQRRKELMIVCDGEKA 60

Query: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120
           AAAEMRK+KMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA      KKKKVKNEE
Sbjct: 61  AAAEMRKKKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA------KKKKVKNEE 120

Query: 121 DILDLNLSL 130
           DILDLNLSL
Sbjct: 121 DILDLNLSL 123

[Close]

BLAST of Carg13402 vs. ExPASy TrEMBL
Match: A0A6J1H831 (protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 9.3e-55
Identity = 126/133 (94.74%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVAS----AAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCN 60
           MEKQSLKTTGKRVAS    AAVDG  EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC+
Sbjct: 1   MEKQSLKTTGKRVASAAAAAAVDG--EEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCD 60

Query: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120
           GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV
Sbjct: 61  GEKAAAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKV 120

Query: 121 KNEEDILDLNLSL 130
           KNEEDILDLNLSL
Sbjct: 121 KNEEDILDLNLSL 131

[Close]

BLAST of Carg13402 vs. ExPASy TrEMBL
Match: A0A6J1KR57 (protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 9.0e-50
Identity = 115/129 (89.15%), Postives = 119/129 (92.25%), Query Frame = 0


Query: 1   MEKQSLKTTGKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKA 60
           MEKQSLKTTGKRVA+A VD EEE+EEEMKMEAFF LVRNFKEMRDQRRKELMI C+GEKA
Sbjct: 1   MEKQSLKTTGKRVAAATVDREEEKEEEMKMEAFFDLVRNFKEMRDQRRKELMIVCDGEKA 60

Query: 61  AAAEMRKRKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAAAIATAKKKKKVKNEE 120
           AAAEMRK+KMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA      KKKKVKNEE
Sbjct: 61  AAAEMRKKKMKTVENRSAWIPRFEREDFDEEFQVRGPAKEEAAAA------KKKKVKNEE 120

Query: 121 DILDLNLSL 130
           DILDLNLSL
Sbjct: 121 DILDLNLSL 123

[Close]

BLAST of Carg13402 vs. ExPASy TrEMBL
Match: A0A6J1DAZ5 (protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.4e-20
Identity = 70/121 (57.85%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 22  EEEEEEMKMEAFFALVRNFKEMRDQRRKEL--MIGCNGEKAAAAEMRKRKMKTVENRSAW 81
           EEEEEEMKMEAFFAL+RNFKEMRD+RRKEL  MIG  GE     E +++KMKT    S W
Sbjct: 12  EEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGE----TEKKRKKMKTAP--STW 71

Query: 82  IPRFEREDFDEEFQVRGP----------AKEEAAAAAI-ATAKKKKKVKNEEDILDLNLS 130
           IP F REDFDE+FQVRGP           KE+     + A   KKK +KN+E+ LDLNL+
Sbjct: 72  IPTFLREDFDEDFQVRGPPPVLPKPCNLIKEDTPPPPLPAAVAKKKNMKNDEECLDLNLA 126

[Close]

BLAST of Carg13402 vs. ExPASy TrEMBL
Match: A0A6J1JVD5 (uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.6e-17
Identity = 65/119 (54.62%), Postives = 82/119 (68.91%), Query Frame = 0


Query: 22  EEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKAAAAEMRKR--KMKTVENRSAW 81
           EEEEEE++ME F+ALVRNFKE+RD+ RKE++ G  G++ AA + RKR    +TV+N+S+W
Sbjct: 15  EEEEEEVEMEKFYALVRNFKEIRDRWRKEVVNG-EGDETAAEKTRKRMKTTETVQNQSSW 74

Query: 82  IPRFEREDFDEEFQVRGPAKE---------EAAAAAIATAKKKKKVKNEEDILDLNLSL 130
           I RFEREDFDE+ QV G A E         E    A A  KK+KKV +  D LDLNLSL
Sbjct: 75  ILRFEREDFDEDLQVGGAAVELPKHCCLVKEDTPVAAAPLKKEKKVASGVDCLDLNLSL 132

[Close]

BLAST of Carg13402 vs. ExPASy TrEMBL
Match: A0A0A0K5V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.8e-16
Identity = 69/137 (50.36%), Postives = 83/137 (60.58%), Query Frame = 0


Query: 10  GKRVASAAVDGEEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGC--NGEKAAAAEMRK 69
           GK   S     E+  E+E +ME FFALVR+ KEMRDQRRKEL IG     E+ AA E R+
Sbjct: 4   GKLQPSPDTTAEDSLEDE-EMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEKRR 63

Query: 70  RKMKT---VENRSAWIPRFEREDFDEEFQVRGP---------AKEEAAAAAIATA---KK 129
           ++M+    VENRS WIP+FEREDFDEEFQ              K +  AA+  T    K 
Sbjct: 64  KRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPMKN 123

[Close]

BLAST of Carg13402 vs. TAIR 10
Match: AT1G02450.1 (NIM1-interacting 1 )

HSP 1 Score: 41.6 bits (96), Expect = 5.7e-04
Identity = 34/114 (29.82%), Postives = 61/114 (53.51%), Query Frame = 0


Query: 21  EEEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCNGEKAAAAEMRKRKMKTVENRSAWI 80
           +EEEEEE K++ FF L+++++E R +RR+EL        A  + + +RK    E     +
Sbjct: 37  DEEEEEEKKIDTFFKLIKHYQEARKRRREEL--------AENSGVVRRKSNGGERSGIVV 96

Query: 81  PRFEREDFDE-EFQVRGP----AKEEAAAAAIATAKKKKKVKNEEDILDLNLSL 130
           P F+ EDF +    ++ P    +  +     +   + + + +NE+  LDLNL+L
Sbjct: 97  PAFQPEDFSQCRTGLKPPLMFVSDHKEENTKVEQEEDQTEERNEDKALDLNLAL 142

The following BLAST results are available for this feature:

BLAST of Carg13402 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025612.1	3.4e-59	100.00	hypothetical protein SDJN02_12109, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6593261.1	2.7e-56	97.67	hypothetical protein SDJN03_12737, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022960173.1	1.9e-54	94.74	protein NIM1-INTERACTING 1 [Cucurbita moschata]	[more]
XP_023514605.1	2.6e-51	93.02	protein NIM1-INTERACTING 1 [Cucurbita pepo subsp. pepo]	[more]
XP_023004697.1	1.9e-49	89.15	protein NIM1-INTERACTING 1 [Cucurbita maxima]	[more]

BLAST of Carg13402 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg13402 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H831	9.3e-55	94.74	protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV...	[more]
A0A6J1KR57	9.0e-50	89.15	protein NIM1-INTERACTING 1 OS=Cucurbita maxima OX=3661 GN=LOC111497915 PE=3 SV=1	[more]
A0A6J1DAZ5	7.4e-20	57.85	protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 S...	[more]
A0A6J1JVD5	2.6e-17	54.62	uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189...	[more]
A0A0A0K5V7	3.8e-16	50.36	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1	[more]

BLAST of Carg13402 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02450.1	5.7e-04	29.82	NIM1-interacting 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031425	NPR1/NH1-interacting protein	PFAM	PF15699	NPR1_interact	coord: 22..125 e-value: 4.2E-18 score: 66.0
IPR031425	NPR1/NH1-interacting protein	PANTHER	PTHR33669	PROTEIN NEGATIVE REGULATOR OF RESISTANCE	coord: 7..129
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR33669:SF1	NPR1/NH1-INTERACTING PROTEIN-RELATED	coord: 7..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13402-RA	Carg13402-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010112	regulation of systemic acquired resistance
cellular_component	GO:0005634	nucleus