Carg13384 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg13384 vs. NCBI nr
Match: KAG7025594.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.6 bits (1394), Expect = 3.9e-150
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD
Sbjct: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVLNKMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLNKMSATQCPKA 267

BLAST of Carg13384 vs. NCBI nr
Match: KAG6593243.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 538.5 bits (1386), Expect = 3.3e-149
Identity = 265/267 (99.25%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPPATIPSAALR SSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD
Sbjct: 1   MKPVALLLHPPATIPSAALRFSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVLNKMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLNKMSATQCPKA 267

BLAST of Carg13384 vs. NCBI nr
Match: XP_022959652.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 528.1 bits (1359), Expect = 4.4e-146
Identity = 260/267 (97.38%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPV LLLHPPATI SAALR SSKSHLR RPL+ SCFAVQSESTKVEVSSKPEYKPGMLD
Sbjct: 1   MKPVVLLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSESTKVEVSSKPEYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVLNKMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLNKMSATQCPKA 267

BLAST of Carg13384 vs. NCBI nr
Match: XP_023514967.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.8 bits (1353), Expect = 2.2e-145
Identity = 259/267 (97.00%), Postives = 263/267 (98.50%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPPATI SAALR SSKSHLR RPL+ SCFAVQSESTKVEVSSKPEYKPGMLD
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSESTKVEVSSKPEYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICP+ATRRREVL+KMSATQCPKA
Sbjct: 241 PCLEICPHATRRREVLSKMSATQCPKA 267

BLAST of Carg13384 vs. NCBI nr
Match: XP_023004624.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita maxima])

HSP 1 Score: 523.9 bits (1348), Expect = 8.3e-145
Identity = 258/267 (96.63%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPPATI SAALR SSKSHLR RPL+FSCFAVQSESTKVEVSSKP+YKPGMLD
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNFSCFAVQSESTKVEVSSKPDYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTA TCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGV PPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVPPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVL+KMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLSKMSATQCPKA 267

BLAST of Carg13384 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.3e-28
Identity = 77/236 (32.63%), Postives = 124/236 (52.54%), Query Frame = 0

Query: 20  RLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLDDFFLNL---FRSKMVQE-- 79
           R S    L ++P+         E+ ++E S+       + D FF  +   + SK +Q+  
Sbjct: 29  RSSISPTLCSKPVYSGKLKAAKETARIETSNTK--NASIEDSFFSKIAINYLSKNLQDAA 88

Query: 80  -VGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIAL---FPPLLLELYRMLVSP 139
            +   S    YD L++ A R++     N DT +    +L +L    P ++  L +M   P
Sbjct: 89  GISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPP 148

Query: 140 IAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGIC 199
               KV+  + A  T  +  WL+G   V   E+ +G   +S V++EKC++LE+S C+G+C
Sbjct: 149 ---SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRKEKSVVYIEKCRFLEQSNCVGMC 208

Query: 200 LNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKEPCLEIC 247
            + CK P+Q F KN +G+P+ MEP+FND SC+  FG  PP  E+D  +K+PC E C
Sbjct: 209 THICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFEFC 254

BLAST of Carg13384 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.3e-26
Identity = 72/214 (33.64%), Postives = 109/214 (50.93%), Query Frame = 0

Query: 54  YKPGMLDDFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILI 113
           YK    D   +N     +    G  ++K G+D L+E A   + K     +T +    IL 
Sbjct: 53  YKDNWFDKLAINHLSKSVQAATGISNNKSGFDSLVEAATVASQK----FNTTQQQGIILD 112

Query: 114 AL---FPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQ 173
           AL   FP  +L + R ++ P    K+A    A  T     WL+G   V   E+ +G   +
Sbjct: 113 ALDRAFPKPILSVIRRVMPP---SKLAREYFAVFTTIFFAWLLGPSEVRESEI-NGRREK 172

Query: 174 SGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPP 233
           + V ++KC++LEE+ C+G+C+N CK P+Q F K+  G+P+ M PNF+D SC+  FG  PP
Sbjct: 173 NIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDMSCEMIFGQEPP 232

Query: 234 LPEEDSILKEPCLEICPNATRRREVLNKMSATQC 265
              +D  LK+PC ++C           K  ATQC
Sbjct: 233 ASTDDPALKQPCYKLCK--------AKKNHATQC 250

BLAST of Carg13384 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-22
Identity = 61/211 (28.91%), Postives = 105/211 (49.76%), Query Frame = 0

Query: 38  AVQSESTKVEVSSKPE---YKPGMLDDFFLNLFRSKMVQEVGWDSDKPGYDGLIEVA--- 97
           A    + + E ++ P    Y+    D   +      + +  G  ++K GY+ LI+ A   
Sbjct: 61  AAMMSTVQTETAAAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAI 120

Query: 98  NRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQW 157
           +R+    K +    +A  R L    P  +L + ++++ P    + +    A  T     W
Sbjct: 121 SRIFSLDKQSEIVTQALERAL----PSYILTMIKVMMPP---SRFSREYFAAFTTIFFPW 180

Query: 158 LMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPLL 217
           L+G C V   E+ +G   ++ V++ KC++LE + C+G+C N CK P Q F ++ +G+ + 
Sbjct: 181 LVGPCEVMESEV-EGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVY 240

Query: 218 MEPNFNDYSCQFKFGVLPPLPEEDSILKEPC 243
           M PNF D SC+  FG  P  PE+D  LK+PC
Sbjct: 241 MSPNFEDMSCEMIFGQQP--PEDDPALKQPC 261

BLAST of Carg13384 vs. ExPASy TrEMBL
Match: A0A6J1H551 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460668 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.1e-146
Identity = 260/267 (97.38%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPV LLLHPPATI SAALR SSKSHLR RPL+ SCFAVQSESTKVEVSSKPEYKPGMLD
Sbjct: 1   MKPVVLLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSESTKVEVSSKPEYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVLNKMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLNKMSATQCPKA 267

BLAST of Carg13384 vs. ExPASy TrEMBL
Match: A0A6J1KWU1 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497865 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 4.0e-145
Identity = 258/267 (96.63%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPPATI SAALR SSKSHLR RPL+FSCFAVQSESTKVEVSSKP+YKPGMLD
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNFSCFAVQSESTKVEVSSKPDYKPGMLD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLNLFRSKMVQEVGWDS+KPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL
Sbjct: 61  DFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           LELYRMLVSPIAGGKVAAIMVARVTA TCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGV PPLPEEDSILKE
Sbjct: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVPPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREVL+KMSATQCPKA
Sbjct: 241 PCLEICPNATRRREVLSKMSATQCPKA 267

BLAST of Carg13384 vs. ExPASy TrEMBL
Match: A0A0A0K9J2 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407750 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.3e-127
Identity = 229/267 (85.77%), Postives = 242/267 (90.64%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 60
           MKPVALLLHPP   P+     SSKSHLRNRP   SC A+QS S K   SSK EYKPG+LD
Sbjct: 1   MKPVALLLHPPTISPAPLRFTSSKSHLRNRPFILSCSALQSGSIKDGASSKAEYKPGILD 60

Query: 61  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 120
           DFFLN+FRSKMVQEVGWDS+KPGYDGLIEVA+RLTM GK+NS+TIEASVRILIALFPPLL
Sbjct: 61  DFFLNVFRSKMVQEVGWDSEKPGYDGLIEVASRLTMTGKTNSETIEASVRILIALFPPLL 120

Query: 121 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180
           L+LYR+LVSPIAGGKVAAIMVARVTA TCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY
Sbjct: 121 LKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 180

Query: 181 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 240
           LEESKCIGIC+NTCK PTQSFFK+QMGIPLLMEPNF DYSCQFKFGVLPPLPEEDSILKE
Sbjct: 181 LEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFTDYSCQFKFGVLPPLPEEDSILKE 240

Query: 241 PCLEICPNATRRREVLNKMSATQCPKA 268
           PCLEICPNATRRREV  K+SA QCPKA
Sbjct: 241 PCLEICPNATRRREVSGKISAAQCPKA 267

BLAST of Carg13384 vs. ExPASy TrEMBL
Match: A0A5D3D2R7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001770 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 232/268 (86.57%), Postives = 245/268 (91.42%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRL-SSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGML 60
           MKP+ALLLHPP TI SA LR  SSKSHLRNRP   SC A+QS S K   SSK EYKPG+L
Sbjct: 1   MKPMALLLHPP-TISSAPLRFNSSKSHLRNRPFILSCSALQSGSIKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGY GLIEVA+RLTM G++NS+TIEASVRIL ALFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTA TCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240
           YLEESKCIGIC+NTCK PTQSFFK+QMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVLNKMSATQCPKA 268
           EPCLEICPNATRRREV  KMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Carg13384 vs. ExPASy TrEMBL
Match: A0A1S3BRJ4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492751 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 232/268 (86.57%), Postives = 245/268 (91.42%), Query Frame = 0

Query: 1   MKPVALLLHPPATIPSAALRL-SSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGML 60
           MKP+ALLLHPP TI SA LR  SSKSHLRNRP   SC A+QS S K   SSK EYKPG+L
Sbjct: 1   MKPLALLLHPP-TISSAPLRFNSSKSHLRNRPFILSCSALQSGSIKDGTSSKSEYKPGIL 60

Query: 61  DDFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPL 120
           DDFFLN+FRSKMVQEVGWDS+KPGY GLIEVA+RLTM G++NS+TIEASVRIL ALFPPL
Sbjct: 61  DDFFLNVFRSKMVQEVGWDSEKPGYGGLIEVASRLTMTGRTNSETIEASVRILTALFPPL 120

Query: 121 LLELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180
           LL+LYR+LVSPIAGGKVAAIMVARVTA TCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK
Sbjct: 121 LLKLYRILVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCK 180

Query: 181 YLEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240
           YLEESKCIGIC+NTCK PTQSFFK+QMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK
Sbjct: 181 YLEESKCIGICINTCKLPTQSFFKDQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILK 240

Query: 241 EPCLEICPNATRRREVLNKMSATQCPKA 268
           EPCLEICPNATRRREV  KMSA QCPKA
Sbjct: 241 EPCLEICPNATRRREVSGKMSAAQCPKA 267

BLAST of Carg13384 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 293.9 bits (751), Expect = 1.3e-79
Identity = 151/267 (56.55%), Postives = 192/267 (71.91%), Query Frame = 0

Query: 4   VALLLHPPATI---PSAALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLD 63
           + +LL PP TI    S + +L     LR R       +  SE    E + K EYKPG LD
Sbjct: 5   IQVLLPPPTTIVFSNSFSTKLILTRRLRCR------ISNSSEVKSDEGAPKLEYKPGPLD 64

Query: 64  DFFLNLFRSKMVQEVGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLL 123
           DFF+  FR+K+V+EVG DS+KPGY GLIE+   L +KG++ S+T +A+VRIL +LFPPL+
Sbjct: 65  DFFMQSFRNKLVEEVGSDSEKPGYVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLI 124

Query: 124 LELYRMLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKY 183
           LELY++L++PIA GK+AA+MVARVT  TCQWLMG   VN I+LP+G S  SGVFVEKC+Y
Sbjct: 125 LELYKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQY 184

Query: 184 LEESKCIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKE 243
           LEESKC+G+C+NTCK PTQ+FFK+ MG+PL+MEPNF DYSCQFKFGV P  PE+D  + E
Sbjct: 185 LEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAP--PEDDGNVNE 244

Query: 244 PCLEICPNATRRREVLNKMSATQCPKA 268
           PC E C  A RR     K+ + +CP A
Sbjct: 245 PCFETCSIAGRR-----KLKSGECPLA 258

BLAST of Carg13384 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 143.3 bits (360), Expect = 2.9e-34
Identity = 89/257 (34.63%), Postives = 133/257 (51.75%), Query Frame = 0

Query: 17  AALRLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLDDFFLNLFRSKM----- 76
           AA+      +L  R  S  C   +       +  K  Y+ G+++  F+ LF  KM     
Sbjct: 2   AAIASLQAVNLTFRRRSTRCGIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGS 61

Query: 77  -----VQEVG-WDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIALFPPLLLELYR 136
                 +E G W+ D   Y+  +EV+ R+ M+G+S     EA   +L+++ PP   E +R
Sbjct: 62  KKKKDTKEKGFWEYD---YESFVEVSKRV-MQGRSRVQQQEAVREVLLSMLPPGAPEQFR 121

Query: 137 MLVSPIAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESK 196
            L  P    K AA   A +T     WL+G   V  +E+ +G   +SGV ++KC+YLE S 
Sbjct: 122 KLFPPT---KWAAEFNAALTVPFFHWLVGPSQVIEVEV-NGVKQRSGVRIKKCRYLENSG 181

Query: 197 CIGICLNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKEPCL-E 256
           C+G+C+N CK PTQ FF N+ G+PL M PN+ D SC+  +G  PP  EED   K+PCL +
Sbjct: 182 CVGMCVNMCKIPTQDFFTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPCLAD 241

Query: 257 ICPNATRRREVLNKMSA 262
           IC  +     +  K+ A
Sbjct: 242 ICSMSNPSSPICPKLEA 250

BLAST of Carg13384 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-29
Identity = 77/236 (32.63%), Postives = 124/236 (52.54%), Query Frame = 0

Query: 20  RLSSKSHLRNRPLSFSCFAVQSESTKVEVSSKPEYKPGMLDDFFLNL---FRSKMVQE-- 79
           R S    L ++P+         E+ ++E S+       + D FF  +   + SK +Q+  
Sbjct: 29  RSSISPTLCSKPVYSGKLKAAKETARIETSNTK--NASIEDSFFSKIAINYLSKNLQDAA 88

Query: 80  -VGWDSDKPGYDGLIEVANRLTMKGKSNSDTIEASVRILIAL---FPPLLLELYRMLVSP 139
            +   S    YD L++ A R++     N DT +    +L +L    P ++  L +M   P
Sbjct: 89  GISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPP 148

Query: 140 IAGGKVAAIMVARVTAFTCQWLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGIC 199
               KV+  + A  T  +  WL+G   V   E+ +G   +S V++EKC++LE+S C+G+C
Sbjct: 149 ---SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRKEKSVVYIEKCRFLEQSNCVGMC 208

Query: 200 LNTCKFPTQSFFKNQMGIPLLMEPNFNDYSCQFKFGVLPPLPEEDSILKEPCLEIC 247
            + CK P+Q F KN +G+P+ MEP+FND SC+  FG  PP  E+D  +K+PC E C
Sbjct: 209 THICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFEFC 254