Carg13345 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13345
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionchaperone protein dnaJ 72
LocationCarg_Chr07: 6598909 .. 6603027 (-)
RNA-Seq ExpressionCarg13345
SyntenyCarg13345
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAACTTTCTTCCGAGAAGCCAGAGTTTCGATTCCATTCCATGGAAGCTCGTCGACTCCGGAATTTCCTGAAGAAGAAGCGATTCCCATCGTCGTCAACTCCATTTCTTGAAGGATTCATCTCCCGCCGCTGTAATTGTGAACGGAGAGAATAATCGGCCATGGATGTTCGCGATCACTACAAGGTACTGGGCTTGAACAGAAGCGCGACGAAGGAAGAAGTCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCACTCCCAATCGCCCAAGTCCGTTAGGGACTCCGCTACTTTGAGATTCAAACAGGTTTCGGAAGCGTATGAGATTCTTGGCGATGATTGTAAGCGTGCTGATTATAATATTCGATCTCGTTGTGCTTCTGGTCCGTCTGTTAATCAACATTATTACTCGTCGTATAATTCCTATGCTAACGCGAGTGGACCTCGATATGGATATGGTTCTTCCTCTGGATTTGCCTCTCGCTCTGGTTCTAATGTCGATGGCTTGTTCACGAATTTTCATATGCTGCTCCGGTTTCTCACCACGCGTGCGTTTCTTCTCAATTTGGCTTTCGCCGGGTATTCATTCGTTACCCTAATATTTTCTTCCAATACGATTTATGTTTTGAATTCTGATAGCAACTTGCTTCGACGCTTACTTTCGCTAGAGAAATGCTCCTCTTTCCATGTCTAAATTTCCGTTTCGGAAACAAGTGTATATCTGTTGAAGAAAGCGTAAATGTATCTGCACTTCTGCAATCCTGAATCTTGATTTTGCATCCTATTTTTCAATTAGTTGATTGGATATATTTGTCACCGTTGATTGGTTCTTCACTTTTTCAATTCAAGCTTTTGGATTATATCCTGGTGCTTCTTCATCTACGCTGTTTTCAGCCCAAAATTTATGCCCCGATTCTCCACTTAAGCTTCAGTGTTCGTTATAATTTAGTATGTGAATTTGCTCGATGATGATACTGCAATACTTCATGTAAAACTATTAATTCTTTTAAACGTTTTTTGAATTCTTGTATCATTTTTACCCTTCCAAAGATCAGTGTGTATACCCTTCCTTAACTAAATTATACACAGAATAGTGTGTATTTGATGGACAACATGTGAATATTCTTAGCTAAACCCCTTGGTTCAATGGTAGCAAGAATTTCTTGGAACATTTGATTTGTGGGGAGGGTTTTTGTTCCCTGGATAAGGTCGACAAACCAGTTCTTACCGTCGTATGGATCTTCTTTTTTCCCCCGAAACAATGGAATCTGAAAGTTGCCCCAAGTAGAAATTTGAGAATGGACAAAAGGTTGGCTGCTCACTAGAGAGGAAAGGGACTGAAAACAAAACAAATCAGAGGAGCCAAAAAATTGGACAATTCTAGAACCGTGACCGAATATTTAAGGAACCGTGACTGAATGTTTCAAAAATTACCTTTTTAGTTTTCAAAATTTGGCTTGATTGTTGGTATATGTAAAAAGTAGACAATTAAAGAAAGAAACTTATTAGTGAAAGTAGTGTATAAGCTTAATTTTCAAAACCAAAGATAAATATAGTTTTCAAACGGAGCTTATACTATTTTATTTTCTTGCATTAGACATGGACGTTGGTTTCATAGTCAAGCTGTGAATATGAACTCTGCTGTATGATTTGGTTATTGTATGTAAAATTGAGAATAATTTATGAGTTTGAGTTTATCTTGGAGCCTTCTTCAACTCCAATTATACTCTTTCAATGTTTACAGTGTCTGAGTTTATTGAGCCTGTGCTCTCTGACTGGGAAGTGTTACATTTTTTTGCAGTGTTTTAGTTGGTGGCATGTTTGCAATTGACACAAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGGTATGTAATTATTTGTTCTCTTCCCCACCCTCGCTCCCAATTCACCCCTACCAAAATGACACATTTCCAGTGATGAAATATCCTGCTCTTTTGCAGATTTTGCGATTCATACTTGTATGTTCTTATTAATAGTTTGAGCATAGTTACGACTTTGGTCTCTGAACTTTCATACTTGTCTTGGCCTTTACGTTTTCAAATATTATGTTTTATTCTCTAAACCTTGCATAAAAAACTTGAATCATACTTGTATGATCTTATTAATAGTTTGTCCCTGAATTTTCTTACTTGTCTTGGCCTTTACGTTTTCAAATATTATGTTTTATTCTCTAAACCTTGCATAAAAAACTCAAATCATACTGTATGCTTATTAATAGTTTTGAGCATAGTTGCGACTTTGGTCCCTGAATTTTCATACTTGTCTTGGCTTTGCGTTTTCAAATATTATGTTTTATTCTCTAAACCTTGCATAAAAAACTTGAATCGTACTTCAATCATACTTGTATGTTCTTATTAATAGTTTTGAGCATAGTTACGACTTTGGTCCTTGAATTTTCATACTTGTCTTGGCCTTTACGCTTTCAAATATTATGTTTTATTCTCTAAACCTTGCATAAAAAACTCGAATCATACTTGTATGTTCTTATTAATAGTTTTGAGCATAGCTACGACTTTGGTCCCTTAATTTTCATAGTTGTCTTGACCTTTACGTTTTCAAATATTATGTTTATTCTCTAAGCCTTGCATAAAAAACTCGAATCATACTTGTATGTTTTTATTAATAGTTTTGAGCATAGTTACAACTTTGGTCCCTAGCATAGTCCCTTAATTTTCATGCTTGTCTTGGCCTTTGCGTTTTCAAATATTATGTTTTATTCTCTAGACCTTGCATAAAAAACTAGCCTTCGTCATTGAACTTTTGATCCTTTGACAAAAAACTTCATTCATTTGGAAACTTAGACACTTGACCTACTATGGGGATTTGAACAATTGTCCATGTAGACACATGTTGATGAGATAATAAAAAATGTGCCATTGTCCGAATGGTTCGTGGAGTAAAACTTAATGTTTAAAAGCTTATAAACCAAAATAGAATAAGCACAAATATGTTATTAGTAGACTCTTAAACCATATAGGCGTTTAAAATTACTTCTAAAACAAAGTATATTATTCATAGATTTTACAAACCGTGATCCACGTTGGTTGGGGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAATCTCTCCTTAGCAGCTGCGTTTTAAAAACCTTGATGAGAGGAAGCCTTGAAAGGGATAGTCCAAAGAGAACAATATCTGCTAGCAGTGGACTTGTGTTGTTACATAGAGACAAACTGTTTGTCTCTATTGCAAACTATTCTAGTAATGTTTGTGTTTATTGAATGCTATCTGTGACTGGAAATGTTTGTTTTCCTTACAGAAATCATTTGAAGAAGCCATGGAATCAATCAATAAAGCCAAAACACAAGAAGATGCTTAAAGGGCTTGCATTGGAAAGTCATTTCCCTACCACCGGATGGCGTTTTCATGTCTTCTTCCTGTGGAGATCACATTTCTCAATGTAAGTGAAGTTGAAACTGACTGGACTGTACAAAGAATCGGAGAGCTGCATGAAATGTACATATACTAATCTTTTTCTTAACCCCTCCTGCTTCATGTCATTATAGATCTATAGATTTTATGAAACTTTTTTAAGTGTTGTCTGCATTTTCATGTGGCGCAGTGTTGTCTGGTGTGACAAATTTCGCCATGAATTCGAGCATACTCTAGTGGATAAGGTATCGATTACGATATTATAGATTTTTGTTGAACTAAAAATAATGTATTGCAGGGTAGGGGTGTTGTATTCGTTTAGAAGAAAAATAAAAATTGTGTGTTCATTGTAGTCTTATAGAACGACAATTATTTAAATATGTTTGTATGGCCGGAAAAGCCAAGGATCTAACTTATCAAGTTTTACTACAGTTACTTGAAATGTGTTTGGATAACGTCTTTTTTTCGATTGGGGGATGGGGAGGAGAAGCAGAATGATATTTCTGTAGGATTTGTGTGTTAAAAGATGGGCTTTTTGGGACTTCTATTGTCTGTCATTTTCAATTTTGTCTCTATTTTTTTTCTTTAAAAAATTATTCTGATTTTCTCTTCATGTATTAATTCTTTTAATCAAACTATACTTTGAAAAAAATGGGTTTATGAGATATTTCATCTAGCCT

mRNA sequence

TAACTTTCTTCCGAGAAGCCAGAGTTTCGATTCCATTCCATGGAAGCTCGTCGACTCCGGAATTTCCTGAAGAAGAAGCGATTCCCATCGTCGTCAACTCCATTTCTTGAAGGATTCATCTCCCGCCGCTGTAATTGTGAACGGAGAGAATAATCGGCCATGGATGTTCGCGATCACTACAAGGTACTGGGCTTGAACAGAAGCGCGACGAAGGAAGAAGTCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCACTCCCAATCGCCCAAGTCCGTTAGGGACTCCGCTACTTTGAGATTCAAACAGGTTTCGGAAGCGTATGAGATTCTTGGCGATGATTGTAAGCGTGCTGATTATAATATTCGATCTCGTTGTGCTTCTGGTCCGTCTGTTAATCAACATTATTACTCGTCGTATAATTCCTATGCTAACGCGAGTGGACCTCGATATGGATATGGTTCTTCCTCTGGATTTGCCTCTCGCTCTGGTTCTAATGTCGATGGCTTGTTCACGAATTTTCATATGCTGCTCCGGTTTCTCACCACGCGTGCGTTTCTTCTCAATTTGGCTTTCGCCGGTGTTTTAGTTGGTGGCATGTTTGCAATTGACACAAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGAAATCATTTGAAGAAGCCATGGAATCAATCAATAAAGCCAAAACACAAGAAGATGCTTAAAGGGCTTGCATTGGAAAGTCATTTCCCTACCACCGGATGGCGTTTTCATGTCTTCTTCCTGTGGAGATCACATTTCTCAATGTAAGTGAAGTTGAAACTGACTGGACTGTACAAAGAATCGGAGAGCTGCATGAAATGTACATATACTAATCTTTTTCTTAACCCCTCCTGCTTCATGTCATTATAGATCTATAGATTTTATGAAACTTTTTTAAGTGTTGTCTGCATTTTCATGTGGCGCAGTGTTGTCTGGTGTGACAAATTTCGCCATGAATTCGAGCATACTCTAGTGGATAAGGTATCGATTACGATATTATAGATTTTTGTTGAACTAAAAATAATGTATTGCAGGGTAGGGGTGTTGTATTCGTTTAGAAGAAAAATAAAAATTGTGTGTTCATTGTAGTCTTATAGAACGACAATTATTTAAATATGTTTGTATGGCCGGAAAAGCCAAGGATCTAACTTATCAAGTTTTACTACAGTTACTTGAAATGTGTTTGGATAACGTCTTTTTTTCGATTGGGGGATGGGGAGGAGAAGCAGAATGATATTTCTGTAGGATTTGTGTGTTAAAAGATGGGCTTTTTGGGACTTCTATTGTCTGTCATTTTCAATTTTGTCTCTATTTTTTTTCTTTAAAAAATTATTCTGATTTTCTCTTCATGTATTAATTCTTTTAATCAAACTATACTTTGAAAAAAATGGGTTTATGAGATATTTCATCTAGCCT

Coding sequence (CDS)

ATGGATGTTCGCGATCACTACAAGGTACTGGGCTTGAACAGAAGCGCGACGAAGGAAGAAGTCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCACTCCCAATCGCCCAAGTCCGTTAGGGACTCCGCTACTTTGAGATTCAAACAGGTTTCGGAAGCGTATGAGATTCTTGGCGATGATTGTAAGCGTGCTGATTATAATATTCGATCTCGTTGTGCTTCTGGTCCGTCTGTTAATCAACATTATTACTCGTCGTATAATTCCTATGCTAACGCGAGTGGACCTCGATATGGATATGGTTCTTCCTCTGGATTTGCCTCTCGCTCTGGTTCTAATGTCGATGGCTTGTTCACGAATTTTCATATGCTGCTCCGGTTTCTCACCACGCGTGCGTTTCTTCTCAATTTGGCTTTCGCCGGTGTTTTAGTTGGTGGCATGTTTGCAATTGACACAAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGAAATCATTTGAAGAAGCCATGGAATCAATCAATAAAGCCAAAACACAAGAAGATGCTTAA

Protein sequence

MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDGLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKTQEDA
Homology
BLAST of Carg13345 vs. NCBI nr
Match: KAG7027464.1 (Chaperone protein dnaJ 72 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-97
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180

Query: 181 AKTQEDA 188
           AKTQEDA
Sbjct: 181 AKTQEDA 187

BLAST of Carg13345 vs. NCBI nr
Match: KAG6595464.1 (Chaperone protein dnaJ 72, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 186/187 (99.47%), Postives = 186/187 (99.47%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180

Query: 181 AKTQEDA 188
           AKT EDA
Sbjct: 181 AKTHEDA 187

BLAST of Carg13345 vs. NCBI nr
Match: XP_022925035.1 (chaperone protein dnaJ 72 [Cucurbita moschata])

HSP 1 Score: 361.7 bits (927), Expect = 3.8e-96
Identity = 185/187 (98.93%), Postives = 185/187 (98.93%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYAN SGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANGSGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180

Query: 181 AKTQEDA 188
           AKT EDA
Sbjct: 181 AKTHEDA 187

BLAST of Carg13345 vs. NCBI nr
Match: XP_023518661.1 (chaperone protein dnaJ 72 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 8.6e-96
Identity = 184/187 (98.40%), Postives = 186/187 (99.47%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPK+VRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKAVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSV QHYYSSYNSYANASGPRYGYGSSSGFA+RSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVKQHYYSSYNSYANASGPRYGYGSSSGFAARSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180

Query: 181 AKTQEDA 188
           AKTQEDA
Sbjct: 181 AKTQEDA 187

BLAST of Carg13345 vs. NCBI nr
Match: XP_022966465.1 (chaperone protein dnaJ 72 [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 2.1e-94
Identity = 182/187 (97.33%), Postives = 185/187 (98.93%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPK+VRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKAVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGP VNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPYVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGG+FAIDTSGEALWKMHNSGKSFEEAM SI+K
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGIFAIDTSGEALWKMHNSGKSFEEAMASIDK 180

Query: 181 AKTQEDA 188
           AKTQEDA
Sbjct: 181 AKTQEDA 187

BLAST of Carg13345 vs. ExPASy Swiss-Prot
Match: Q0WTI8 (Chaperone protein dnaJ 72 OS=Arabidopsis thaliana OX=3702 GN=ATJ72 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.9e-43
Identity = 102/183 (55.74%), Postives = 124/183 (67.76%), Query Frame = 0

Query: 5   DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R+ATK+EVK+AFR+LA ++HPDKH+QSP+ VR +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNQHYYSSY-NSYANASGPRYGYGSSSGFASRSGSNVDGLFT 124
           D KRA YN  S        +  Y + Y N    A G  YGYG      +R  S+     +
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRNRQASSFS---S 122

Query: 125 NFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKT 184
            F    R+LTTRAFLLNLA AG L     AIDTSGE LWKM NSGKSFEEAMESI K+K+
Sbjct: 123 GFDSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGKSFEEAMESIEKSKS 182

Query: 185 QED 187
            +D
Sbjct: 183 HKD 182

BLAST of Carg13345 vs. ExPASy Swiss-Prot
Match: C3P8L9 (Chaperone protein DnaJ OS=Bacillus anthracis (strain A0248) OX=592021 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-13
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 1  MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+E+K+A+R+LAK++HPD       S  ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPD------VSKEENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

BLAST of Carg13345 vs. ExPASy Swiss-Prot
Match: C3L5R6 (Chaperone protein DnaJ OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) OX=568206 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-13
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 1  MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+E+K+A+R+LAK++HPD       S  ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPD------VSKEENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

BLAST of Carg13345 vs. ExPASy Swiss-Prot
Match: Q81LS3 (Chaperone protein DnaJ OS=Bacillus anthracis OX=1392 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-13
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 1  MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+E+K+A+R+LAK++HPD       S  ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPD------VSKEENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

BLAST of Carg13345 vs. ExPASy Swiss-Prot
Match: B7JN38 (Chaperone protein DnaJ OS=Bacillus cereus (strain AH820) OX=405535 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.2e-13
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 1  MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+E+K+A+R+LAK++HPD       S  ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPD------VSKEENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

BLAST of Carg13345 vs. ExPASy TrEMBL
Match: A0A6J1EGR8 (chaperone protein dnaJ 72 OS=Cucurbita moschata OX=3662 GN=LOC111432401 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.9e-96
Identity = 185/187 (98.93%), Postives = 185/187 (98.93%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYAN SGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANGSGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180

Query: 181 AKTQEDA 188
           AKT EDA
Sbjct: 181 AKTHEDA 187

BLAST of Carg13345 vs. ExPASy TrEMBL
Match: A0A6J1HRQ1 (chaperone protein dnaJ 72 OS=Cucurbita maxima OX=3661 GN=LOC111466112 PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.0e-94
Identity = 182/187 (97.33%), Postives = 185/187 (98.93%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPK+VRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKAVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGP VNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPYVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           LFTNFHMLLRFLTTRAFLLNLAFAGVLVGG+FAIDTSGEALWKMHNSGKSFEEAM SI+K
Sbjct: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGIFAIDTSGEALWKMHNSGKSFEEAMASIDK 180

Query: 181 AKTQEDA 188
           AKTQEDA
Sbjct: 181 AKTQEDA 187

BLAST of Carg13345 vs. ExPASy TrEMBL
Match: A0A6J1DJW0 (chaperone protein dnaJ 72 OS=Momordica charantia OX=3673 GN=LOC111021790 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.0e-86
Identity = 171/188 (90.96%), Postives = 175/188 (93.09%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPK++RDSATLRFKQVSEAYE
Sbjct: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYY-SSYNSYANASGPRYGYGSSSGFASRSGSNVD 120
           ILGDDCKRADYNIRSRCASGPSVN HYY SSYNSYANASGPR  Y SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPR--YASSSGFASRSGSNVE 120

Query: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTQEDA 188
           KAKT EDA
Sbjct: 181 KAKTHEDA 186

BLAST of Carg13345 vs. ExPASy TrEMBL
Match: A0A1S3B4T7 (chaperone protein dnaJ 72 OS=Cucumis melo OX=3656 GN=LOC103486026 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.3e-85
Identity = 169/187 (90.37%), Postives = 173/187 (92.51%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGL RSATKEE+K+AFRKLAKEFHPDKHSQSPK VRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLTRSATKEEIKDAFRKLAKEFHPDKHSQSPKVVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQ YYSSYNSYA ASGPR  YGSSSGFASRS  N DG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQQYYSSYNSYARASGPR--YGSSSGFASRSRFNADG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINK 180
           L TNFHMLLRFLTTRAFLLN AFAGVL GGM AID+SGEALWKMHNSGKSFEEAM+SINK
Sbjct: 121 LVTNFHMLLRFLTTRAFLLNFAFAGVLAGGMLAIDSSGEALWKMHNSGKSFEEAMDSINK 180

Query: 181 AKTQEDA 188
           AKT EDA
Sbjct: 181 AKTHEDA 185

BLAST of Carg13345 vs. ExPASy TrEMBL
Match: A0A5A7TEJ7 (Chaperone protein dnaJ 72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G00350 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.2e-64
Identity = 133/151 (88.08%), Postives = 136/151 (90.07%), Query Frame = 0

Query: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60
           MDVRDHYKVLGL RSATKEE+K+AFRKLAKEFHPDKHSQSPK VRDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLTRSATKEEIKDAFRKLAKEFHPDKHSQSPKVVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSSYNSYANASGPRYGYGSSSGFASRSGSNVDG 120
           ILGDDCKRADYNIRSRCASGPSVNQ YYSSYNSYA ASGPR  YGSSSGFASRS  N DG
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQQYYSSYNSYARASGPR--YGSSSGFASRSRFNADG 120

Query: 121 LFTNFHMLLRFLTTRAFLLNLAFAGVLVGGM 152
           L TNFHMLLRFLTTRAFLLN AFAG L   +
Sbjct: 121 LVTNFHMLLRFLTTRAFLLNFAFAGYLFSSL 149

BLAST of Carg13345 vs. TAIR 10
Match: AT2G41000.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.8e-44
Identity = 102/183 (55.74%), Postives = 124/183 (67.76%), Query Frame = 0

Query: 5   DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R+ATK+EVK+AFR+LA ++HPDKH+QSP+ VR +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNQHYYSSY-NSYANASGPRYGYGSSSGFASRSGSNVDGLFT 124
           D KRA YN  S        +  Y + Y N    A G  YGYG      +R  S+     +
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRNRQASSFS---S 122

Query: 125 NFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKT 184
            F    R+LTTRAFLLNLA AG L     AIDTSGE LWKM NSGKSFEEAMESI K+K+
Sbjct: 123 GFDSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGKSFEEAMESIEKSKS 182

Query: 185 QED 187
            +D
Sbjct: 183 HKD 182

BLAST of Carg13345 vs. TAIR 10
Match: AT2G41000.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.5e-37
Identity = 90/167 (53.89%), Postives = 109/167 (65.27%), Query Frame = 0

Query: 5   DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R+ATK+EVK+AFR+LA ++HPDKH+QSP+ VR +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNQHYYSSY-NSYANASGPRYGYGSSSGFASRSGSNVDGLFT 124
           D KRA YN  S        +  Y + Y N    A G  YGYG      +R  S+     +
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRNRQASSFS---S 122

Query: 125 NFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKS 171
            F    R+LTTRAFLLNLA AG L     AIDTSGE LWKM NSG S
Sbjct: 123 GFDSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGVS 166

BLAST of Carg13345 vs. TAIR 10
Match: AT1G59725.1 (DNAJ heat shock family protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.7e-12
Identity = 41/103 (39.81%), Postives = 65/103 (63.11%), Query Frame = 0

Query: 5   DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
           D+Y VL +N SAT++++K+++R+LA ++HPDK   +P S++  A  +FKQ+SEAY++L D
Sbjct: 4   DYYNVLNVNPSATEDDLKKSYRRLAMKWHPDK---NPTSIKQEAEAKFKQISEAYDVLSD 63

Query: 65  DCKRADYNI-----RSRCASGPSVNQHYYSSYNSYANASGPRY 103
             KR  Y+       +   +  S  QH YSS N+    +G RY
Sbjct: 64  PNKRQIYDQYGEDGLTATEATASSQQHNYSSGNNNNYNAGFRY 103

BLAST of Carg13345 vs. TAIR 10
Match: AT4G28480.1 (DNAJ heat shock family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-11
Identity = 33/68 (48.53%), Postives = 50/68 (73.53%), Query Frame = 0

Query: 5  DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
          D+YKVL ++RSA  +++K+A+RKLA ++HPDK+  + K     A  +FKQ+SEAY++L D
Sbjct: 4  DYYKVLQVDRSANDDDLKKAYRKLAMKWHPDKNPNNKK----DAEAKFKQISEAYDVLSD 63

Query: 65 DCKRADYN 73
            KRA Y+
Sbjct: 64 PQKRAVYD 67

BLAST of Carg13345 vs. TAIR 10
Match: AT4G28480.2 (DNAJ heat shock family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-11
Identity = 33/68 (48.53%), Postives = 50/68 (73.53%), Query Frame = 0

Query: 5  DHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYEILGD 64
          D+YKVL ++RSA  +++K+A+RKLA ++HPDK+  + K     A  +FKQ+SEAY++L D
Sbjct: 4  DYYKVLQVDRSANDDDLKKAYRKLAMKWHPDKNPNNKK----DAEAKFKQISEAYDVLSD 63

Query: 65 DCKRADYN 73
            KRA Y+
Sbjct: 64 PQKRAVYD 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027464.13.5e-97100.00Chaperone protein dnaJ 72 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6595464.11.3e-9699.47Chaperone protein dnaJ 72, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925035.13.8e-9698.93chaperone protein dnaJ 72 [Cucurbita moschata][more]
XP_023518661.18.6e-9698.40chaperone protein dnaJ 72 [Cucurbita pepo subsp. pepo][more]
XP_022966465.12.1e-9497.33chaperone protein dnaJ 72 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q0WTI83.9e-4355.74Chaperone protein dnaJ 72 OS=Arabidopsis thaliana OX=3702 GN=ATJ72 PE=2 SV=1[more]
C3P8L97.2e-1350.00Chaperone protein DnaJ OS=Bacillus anthracis (strain A0248) OX=592021 GN=dnaJ PE... [more]
C3L5R67.2e-1350.00Chaperone protein DnaJ OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) OX=568... [more]
Q81LS37.2e-1350.00Chaperone protein DnaJ OS=Bacillus anthracis OX=1392 GN=dnaJ PE=3 SV=1[more]
B7JN387.2e-1350.00Chaperone protein DnaJ OS=Bacillus cereus (strain AH820) OX=405535 GN=dnaJ PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EGR81.9e-9698.93chaperone protein dnaJ 72 OS=Cucurbita moschata OX=3662 GN=LOC111432401 PE=4 SV=... [more]
A0A6J1HRQ11.0e-9497.33chaperone protein dnaJ 72 OS=Cucurbita maxima OX=3661 GN=LOC111466112 PE=4 SV=1[more]
A0A6J1DJW03.0e-8690.96chaperone protein dnaJ 72 OS=Momordica charantia OX=3673 GN=LOC111021790 PE=4 SV... [more]
A0A1S3B4T73.3e-8590.37chaperone protein dnaJ 72 OS=Cucumis melo OX=3656 GN=LOC103486026 PE=4 SV=1[more]
A0A5A7TEJ74.2e-6488.08Chaperone protein dnaJ 72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT2G41000.12.8e-4455.74Chaperone DnaJ-domain superfamily protein [more]
AT2G41000.22.5e-3753.89Chaperone DnaJ-domain superfamily protein [more]
AT1G59725.13.7e-1239.81DNAJ heat shock family protein [more]
AT4G28480.12.4e-1148.53DNAJ heat shock family protein [more]
AT4G28480.22.4e-1148.53DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 7..25
score: 42.43
coord: 47..67
score: 46.75
coord: 25..40
score: 44.16
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 4..67
e-value: 4.1E-24
score: 96.1
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 5..72
e-value: 6.9E-22
score: 77.3
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 5..75
score: 19.718315
IPR001623DnaJ domainCDDcd06257DnaJcoord: 5..64
e-value: 9.05075E-23
score: 84.1341
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 1..78
e-value: 4.7E-24
score: 86.4
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 1..107
NoneNo IPR availablePANTHERPTHR24074:SF34CHAPERONE PROTEIN DNAJ 72coord: 5..176
NoneNo IPR availablePANTHERPTHR24074CO-CHAPERONE PROTEIN DJLAcoord: 5..176
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 52..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13345-RACarg13345-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane