Carg13300 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg13300 vs. NCBI nr
Match: KAG7027419.1 (Gamma-interferon-inducible lysosomal thiol reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555.1 bits (1429), Expect = 3.3e-154
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60
           MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL
Sbjct: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60

Query: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120
           ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF
Sbjct: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120

Query: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180
           TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ
Sbjct: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180

Query: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240
           SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY
Sbjct: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240

Query: 241 LQSDNVGSQVCYVTPPRNATS 262
           LQSDNVGSQVCYVTPPRNATS
Sbjct: 241 LQSDNVGSQVCYVTPPRNATS 261

BLAST of Carg13300 vs. NCBI nr
Match: XP_023518316.1 (gamma-interferon-inducible lysosomal thiol reductase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.3 bits (1414), Expect = 1.8e-152
Identity = 257/261 (98.47%), Postives = 260/261 (99.62%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60
           MGR SRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL
Sbjct: 1   MGRLSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60

Query: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120
           ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF
Sbjct: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120

Query: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180
           TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEA+EMQLEQ
Sbjct: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAIEMQLEQ 180

Query: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240
           SYAYETTQLNPPH+FVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRHY
Sbjct: 181 SYAYETTQLNPPHQFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRHY 240

Query: 241 LQSDNVGSQVCYVTPPRNATS 262
           LQSDNVGSQVCYVTPPRNATS
Sbjct: 241 LQSDNVGSQVCYVTPPRNATS 261

BLAST of Carg13300 vs. NCBI nr
Match: XP_022966572.1 (gamma-interferon-inducible lysosomal thiol reductase isoform X1 [Cucurbita maxima])

HSP 1 Score: 533.5 bits (1373), Expect = 1.0e-147
Identity = 252/262 (96.18%), Postives = 256/262 (97.71%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLM-LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60
           MGR SRWDAPAVTALLM LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG
Sbjct: 1   MGRLSRWDAPAVTALLMLLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60

Query: 61  LISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120
           LI+AVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG
Sbjct: 61  LITAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120

Query: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLE 180
           FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEA+EMQLE
Sbjct: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAIEMQLE 180

Query: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRH 240
           QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRH
Sbjct: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRH 240

Query: 241 YLQSDNVGSQVCYVTPPRNATS 262
           YL+S N GSQVCYVT PRN TS
Sbjct: 241 YLESANGGSQVCYVTSPRNTTS 262

BLAST of Carg13300 vs. NCBI nr
Match: KAG6595409.1 (Gamma-interferon-responsive lysosomal thiol protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496.9 bits (1278), Expect = 1.1e-136
Identity = 235/261 (90.04%), Postives = 237/261 (90.80%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60
           MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL
Sbjct: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60

Query: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120
           ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF
Sbjct: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120

Query: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180
           TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL                        LE+
Sbjct: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL------------------------LEK 180

Query: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240
           SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRHY
Sbjct: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRHY 237

Query: 241 LQSDNVGSQVCYVTPPRNATS 262
           LQSDNVGSQVCYVTPPRNATS
Sbjct: 241 LQSDNVGSQVCYVTPPRNATS 237

BLAST of Carg13300 vs. NCBI nr
Match: XP_023518317.1 (gamma-interferon-inducible lysosomal thiol reductase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494.2 bits (1271), Expect = 6.9e-136
Identity = 234/261 (89.66%), Postives = 236/261 (90.42%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60
           MGR SRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL
Sbjct: 1   MGRLSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60

Query: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120
           ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF
Sbjct: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120

Query: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180
           TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL                        LEQ
Sbjct: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL------------------------LEQ 180

Query: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240
           SYAYETTQLNPPH+FVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRHY
Sbjct: 181 SYAYETTQLNPPHQFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRHY 237

Query: 241 LQSDNVGSQVCYVTPPRNATS 262
           LQSDNVGSQVCYVTPPRNATS
Sbjct: 241 LQSDNVGSQVCYVTPPRNATS 237

BLAST of Carg13300 vs. ExPASy Swiss-Prot
Match: Q9SV73 (Gamma-interferon-responsive lysosomal thiol protein OS=Arabidopsis thaliana OX=3702 GN=GILT PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 7.4e-32
Identity = 72/209 (34.45%), Postives = 105/209 (50.24%), Query Frame = 0

Query: 28  SGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFSCQHG 87
           SGKV +++YYE+LCP C  FIV  L K+F+  L +  +L++ P+GNA +  +   +CQHG
Sbjct: 29  SGKVKLNLYYESLCPGCQEFIVDDLGKIFDYDLYTITDLKLFPFGNAELSDNLTVTCQHG 88

Query: 88  PDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPIDCYR 147
            +EC LNA+EAC +  +PD +  + FI CVE  T       W  C   +       DCY 
Sbjct: 89  EEECKLNALEACALRTWPDQKSQYSFIRCVESDT-----KGWESCVKNSGREKAINDCYN 148

Query: 148 NGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVNDKPLQ 207
               + ++L                         YA +T  L PPH +VPWV +N KPL 
Sbjct: 149 GDLSRKLIL------------------------GYATKTKNLKPPHEYVPWVTLNGKPLD 208

Query: 208 EDYQ---NFMAYVCKAFNG-SPAPDACRS 233
           +  Q   + +A +C A+ G +  P  C S
Sbjct: 209 DSVQSTDDLVAQICNAYKGKTTLPKVCNS 208

BLAST of Carg13300 vs. ExPASy Swiss-Prot
Match: Q9ESY9 (Gamma-interferon-inducible lysosomal thiol reductase OS=Mus musculus OX=10090 GN=Ifi30 PE=1 SV=3)

HSP 1 Score: 103.2 bits (256), Expect = 4.5e-21
Identity = 69/218 (31.65%), Postives = 100/218 (45.87%), Query Frame = 0

Query: 20  LLSPSP-SASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAW--- 79
           LL P P   S  V VS+YYE+LC  C  F+V  L   +   ++  +N+ ++P+GNA    
Sbjct: 47  LLGPRPLPPSPPVRVSLYYESLCGACRYFLVRDLFPTWLM-VMEIMNITLVPYGNAQERN 106

Query: 80  IQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDI 139
           +     F+CQHG  EC LN +EAC +    + E  F  I C+E   + +   +   C  +
Sbjct: 107 VSGTWEFTCQHGELECRLNMVEACLLDKL-EKEAAFLTIVCME--EMDDMEKKLGPCLQV 166

Query: 140 AQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRF 199
                 P        GK                        QL    A  T  L+PPH +
Sbjct: 167 YAPEVSPESIMECATGKR---------------------GTQLMHENAQLTDALHPPHEY 226

Query: 200 VPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSI 234
           VPWV+VN+KPL +D    ++ VC+ + G+  PD C SI
Sbjct: 227 VPWVLVNEKPL-KDPSELLSIVCQLYQGTEKPDICSSI 238

BLAST of Carg13300 vs. ExPASy Swiss-Prot
Match: P13284 (Gamma-interferon-inducible lysosomal thiol reductase OS=Homo sapiens OX=9606 GN=IFI30 PE=1 SV=3)

HSP 1 Score: 99.8 bits (247), Expect = 5.0e-20
Identity = 70/207 (33.82%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 31  VTVSVYYEALCPFCANFIVYHLVKLFENGLI--SAVNLRMIPWGNAWIQPDGA---FSCQ 90
           V V++YYEALC  C  F++    +LF   L+    +N+ ++P+GNA  Q       F CQ
Sbjct: 62  VNVTLYYEALCGGCRAFLIR---ELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKCQ 121

Query: 91  HGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPIDC 150
           HG +EC  N +EAC +    D E  F  I C+E F             D+ +   + +  
Sbjct: 122 HGEEECKFNKVEACVLDEL-DMELAFLTIVCMEEFE------------DMERSLPLCLQL 181

Query: 151 YRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVNDKP 210
           Y  G          SP ++  +   M    MQL  + A  T  L PPH +VPWV VN KP
Sbjct: 182 YAPG---------LSPDTI--MECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKP 239

Query: 211 LQEDYQNFMAYVCKAFNGSPAPDACRS 233
           L ED    +  VC+ + G   PD C S
Sbjct: 242 L-EDQTQLLTLVCQLYQGK-KPDVCPS 239

BLAST of Carg13300 vs. ExPASy Swiss-Prot
Match: A6QPN6 (Gamma-interferon-inducible lysosomal thiol reductase OS=Bos taurus OX=9913 GN=IFI30 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.4e-19
Identity = 65/221 (29.41%), Postives = 101/221 (45.70%), Query Frame = 0

Query: 17  MLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAW- 76
           + L  SP       V VS+YYEALCP C  F++  L   +   ++  +N+ ++P+GNA  
Sbjct: 42  LCLQASPQKPDVPLVNVSLYYEALCPGCREFLIRELFPTWLM-VLEILNVTLVPYGNAQE 101

Query: 77  --IQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCF 136
             +     F+CQHG  EC+LN +EAC +      E+   F+  V    + +       C 
Sbjct: 102 RNVSGKWEFTCQHGERECLLNKVEACLLD---QLEQKIAFLTIVCLEEMDDMEQNLKPCL 161

Query: 137 DI--AQLSAIPI-DCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLN 196
            I   ++SA  I +C     G                        MQL    A  T  L 
Sbjct: 162 QIYAPKVSADSIMECATGNRG------------------------MQLLHINAQLTDALR 221

Query: 197 PPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACR 232
           PPH++VPWV+VN + + +D ++ +  VC+ + G   PD C+
Sbjct: 222 PPHKYVPWVVVNGEHM-KDAEHLLHLVCRLYQGQ-KPDVCQ 232

BLAST of Carg13300 vs. ExPASy Swiss-Prot
Match: Q499T2 (Gamma-interferon-inducible lysosomal thiol reductase OS=Rattus norvegicus OX=10116 GN=Ifi30 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.4e-19
Identity = 67/217 (30.88%), Postives = 103/217 (47.47%), Query Frame = 0

Query: 20  LLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAW---I 79
           LLS  P     V VS+YYE+LC  C  F+V +L   +   ++  +N+ ++P+GNA    +
Sbjct: 53  LLSAPP-----VNVSLYYESLCGACRYFLVRNLFPTWLM-VMEIMNITLVPYGNAQERNV 112

Query: 80  QPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIA 139
                F+CQHG  EC LN +EAC +    + E  F  I C+E   +++   +   C  + 
Sbjct: 113 SGTWEFTCQHGELECKLNKVEACLLDKL-EKEAAFLTIVCME--EMEDMEKKLGPCLQL- 172

Query: 140 QLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFV 199
                              +   SP S+          E+  E   A  T  L PPH +V
Sbjct: 173 ------------------YVPEVSPESIMECATGKRGTELMHEN--AQLTDALQPPHEYV 232

Query: 200 PWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSI 234
           PWV+VN+KPL +  Q  ++ VC+ + G+  PD C S+
Sbjct: 233 PWVLVNEKPLTDPSQ-LLSSVCELYQGTEKPDICSSM 238

BLAST of Carg13300 vs. ExPASy TrEMBL
Match: A0A6J1HNC5 (gamma-interferon-inducible lysosomal thiol reductase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466215 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.0e-148
Identity = 252/262 (96.18%), Postives = 256/262 (97.71%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLM-LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60
           MGR SRWDAPAVTALLM LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG
Sbjct: 1   MGRLSRWDAPAVTALLMLLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60

Query: 61  LISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120
           LI+AVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG
Sbjct: 61  LITAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120

Query: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLE 180
           FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEA+EMQLE
Sbjct: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAIEMQLE 180

Query: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRH 240
           QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRH
Sbjct: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRH 240

Query: 241 YLQSDNVGSQVCYVTPPRNATS 262
           YL+S N GSQVCYVT PRN TS
Sbjct: 241 YLESANGGSQVCYVTSPRNTTS 262

BLAST of Carg13300 vs. ExPASy TrEMBL
Match: A0A6J1F0Q7 (gamma-interferon-inducible lysosomal thiol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111438182 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 7.7e-133
Identity = 231/261 (88.51%), Postives = 233/261 (89.27%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60
           MG  S  DAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL
Sbjct: 1   MGCLSPRDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGL 60

Query: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120
           ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF
Sbjct: 61  ISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGF 120

Query: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQ 180
           TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL                        LE+
Sbjct: 121 TVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL------------------------LEK 180

Query: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHY 240
           SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRHY
Sbjct: 181 SYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRHY 237

Query: 241 LQSDNVGSQVCYVTPPRNATS 262
           LQSDNVGSQVCYVTPPRNATS
Sbjct: 241 LQSDNVGSQVCYVTPPRNATS 237

BLAST of Carg13300 vs. ExPASy TrEMBL
Match: A0A6J1HSI5 (gamma-interferon-inducible lysosomal thiol reductase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466215 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.9e-131
Identity = 229/262 (87.40%), Postives = 232/262 (88.55%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLM-LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60
           MGR SRWDAPAVTALLM LLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG
Sbjct: 1   MGRLSRWDAPAVTALLMLLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60

Query: 61  LISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120
           LI+AVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG
Sbjct: 61  LITAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120

Query: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLE 180
           FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL                        LE
Sbjct: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKL------------------------LE 180

Query: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRH 240
           QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKA+NGSPAPDACRSIMLDDRH
Sbjct: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAYNGSPAPDACRSIMLDDRH 238

Query: 241 YLQSDNVGSQVCYVTPPRNATS 262
           YL+S N GSQVCYVT PRN TS
Sbjct: 241 YLESANGGSQVCYVTSPRNTTS 238

BLAST of Carg13300 vs. ExPASy TrEMBL
Match: A0A5D3CGA6 (Gamma-interferon-inducible lysosomal thiol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00130 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.2e-106
Identity = 188/261 (72.03%), Postives = 207/261 (79.31%), Query Frame = 0

Query: 1   MGRQSRWDAPAVTALLMLLLLSP-SPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENG 60
           MGR     +  +TAL  +LLLS   PSAS KVTVSVYYEALCPFCANF+VYHLVKLFENG
Sbjct: 1   MGRLVHGWSAVLTALCAVLLLSSLPPSASEKVTVSVYYEALCPFCANFVVYHLVKLFENG 60

Query: 61  LISAVNLRMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEG 120
           LISAVNLRMIPWGNAWIQPDG+F CQHGPDECMLN IEACTISIYPD EKHFRFIHCVEG
Sbjct: 61  LISAVNLRMIPWGNAWIQPDGSFFCQHGPDECMLNTIEACTISIYPDIEKHFRFIHCVEG 120

Query: 121 FTVQNRHNEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLE 180
            TVQNRHNEWTKCFDIA+LSAIPIDCYRNGHGK+                        LE
Sbjct: 121 LTVQNRHNEWTKCFDIAKLSAIPIDCYRNGHGKM------------------------LE 180

Query: 181 QSYAYETTQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRH 240
           Q YA ETT+LNPPHRFVPWVIV++ PLQEDYQNFMAY+CKA+ GS  P+AC+SI LD+R 
Sbjct: 181 QYYASETTRLNPPHRFVPWVIVDNHPLQEDYQNFMAYICKAYKGSGVPEACKSINLDNRQ 237

Query: 241 YLQSDNVGSQVCYVTPPRNAT 261
           YL++ + GSQVCY T  RN+T
Sbjct: 241 YLENASGGSQVCYATTTRNST 237

BLAST of Carg13300 vs. ExPASy TrEMBL
Match: A0A0A0L2Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G312830 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.2e-106
Identity = 183/254 (72.05%), Postives = 201/254 (79.13%), Query Frame = 0

Query: 7   WDAPAVTALLMLLLLSPSPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNL 66
           W A A     +LL LS  PSAS KVTVSVYYEALCPFCANF+VYHLVKLF+NGLISAVNL
Sbjct: 8   WSAVATALCALLLFLSLPPSASEKVTVSVYYEALCPFCANFVVYHLVKLFQNGLISAVNL 67

Query: 67  RMIPWGNAWIQPDGAFSCQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRH 126
           RMIPWGNAWIQPDG+F CQHGPDECMLN IEACTIS+YPDTE+HFRFIHCVEG TVQNRH
Sbjct: 68  RMIPWGNAWIQPDGSFFCQHGPDECMLNTIEACTISVYPDTEEHFRFIHCVEGLTVQNRH 127

Query: 127 NEWTKCFDIAQLSAIPIDCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYET 186
           NEWTKCFDIAQLSAIPIDCYRNGHGK+                        LEQ YA ET
Sbjct: 128 NEWTKCFDIAQLSAIPIDCYRNGHGKM------------------------LEQYYASET 187

Query: 187 TQLNPPHRFVPWVIVNDKPLQEDYQNFMAYVCKAFNGSPAPDACRSIMLDDRHYLQSDNV 246
           T+LNPPHRFVPWVIV++ PLQEDYQNFMAY+CKA+ GS  P+AC+SI L +R YL + + 
Sbjct: 188 TRLNPPHRFVPWVIVDNHPLQEDYQNFMAYICKAYKGSAVPEACKSINLKNRQYLDNASG 237

Query: 247 GSQVCYVTPPRNAT 261
           GSQVCY T  RN+T
Sbjct: 248 GSQVCYATTTRNST 237

BLAST of Carg13300 vs. TAIR 10
Match: AT5G01580.1 (gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 235.7 bits (600), Expect = 4.1e-62
Identity = 106/208 (50.96%), Postives = 137/208 (65.87%), Query Frame = 0

Query: 24  SPSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFS 83
           S S+S KVT+S+YYEALCPFCA FIV  L K+FE GLIS+++L+++PWGNA I+PDG   
Sbjct: 20  SLSSSQKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSIDLQLVPWGNAAIRPDGTIL 79

Query: 84  CQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPI 143
           CQHG  EC LNAI AC I+ YPD  KHF +I+C E   ++N+  +W  C ++  LS   +
Sbjct: 80  CQHGEAECALNAIHACAINAYPDVMKHFGYIYCTEQLVLENKLEKWADCLEMVGLSRAAV 139

Query: 144 DCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVND 203
           DCY NG+G                         QLEQ YA ET++L P HRFVPWV+VN+
Sbjct: 140 DCYINGYGN------------------------QLEQRYAEETSELYPAHRFVPWVVVNN 199

Query: 204 KPLQEDYQNFMAYVCKAFNGSPAPDACR 232
            PLQE+YQNF+ YVC A+  +  P+ACR
Sbjct: 200 LPLQENYQNFVMYVCNAYGSNQVPEACR 203

BLAST of Carg13300 vs. TAIR 10
Match: AT1G07080.1 (Thioredoxin superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 6.6e-52
Identity = 89/208 (42.79%), Postives = 130/208 (62.50%), Query Frame = 0

Query: 25  PSASGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFS- 84
           PS+S KV+V +YYE+LCP+C++FIV HL KLFE+ LIS V+L + PWGN  ++ D   + 
Sbjct: 33  PSSSPKVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWGNTKLRSDNVTAV 92

Query: 85  CQHGPDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPI 144
           CQHG  EC L+ +EAC I  +P    HF FI+CVE    ++++++W  C++   L++ P+
Sbjct: 93  CQHGAFECFLDTVEACAIDAWPKVSDHFPFIYCVEKLVTEHKYDKWETCYEKLNLNSKPV 152

Query: 145 -DCYRNGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVN 204
            DC  +GHG                         +L   YA ET  L PPH++VPWV+V+
Sbjct: 153 ADCLSSGHGN------------------------ELALHYAAETNALQPPHKYVPWVVVD 212

Query: 205 DKPLQEDYQNFMAYVCKAFNGSPAPDAC 231
            +PL EDY+NF++Y+CKA+ G+  P AC
Sbjct: 213 GQPLYEDYENFISYICKAYKGNKVPGAC 216

BLAST of Carg13300 vs. TAIR 10
Match: AT4G12890.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 162.9 bits (411), Expect = 3.4e-40
Identity = 82/211 (38.86%), Postives = 115/211 (54.50%), Query Frame = 0

Query: 28  SGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFSCQHG 87
           S KV +++YYE+LCP+C NFIV  L K+F++ L+   +L+++P+GNA I  +   +CQHG
Sbjct: 36  SNKVKINLYYESLCPYCQNFIVDDLGKIFDSDLLKITDLKLVPFGNAHISNNLTITCQHG 95

Query: 88  PDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPIDCYR 147
            +EC LNA+EAC I   PD +  ++FI CVE  T     NEW  C   +       DCY 
Sbjct: 96  EEECKLNALEACGIRTLPDPKLQYKFIRCVEKDT-----NEWESCVKKSGREKAINDCY- 155

Query: 148 NGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVNDKPLQ 207
           NG                        +  +L   YA  T+ L P H +VPWV +N KPL 
Sbjct: 156 NGD-----------------------LSQKLILGYAKLTSSLKPKHEYVPWVTLNGKPLY 215

Query: 208 EDYQNFMAYVCKAFNGSPAPDACRSIMLDDR 239
           ++Y N +A VCKA+ G   P  C S +L +R
Sbjct: 216 DNYHNLVAQVCKAYKGKDLPKLCSSSVLYER 217

BLAST of Carg13300 vs. TAIR 10
Match: AT4G12900.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 156.0 bits (393), Expect = 4.2e-38
Identity = 80/212 (37.74%), Postives = 112/212 (52.83%), Query Frame = 0

Query: 28  SGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFSCQHG 87
           S KV +++YYE+LCP C NFIV+HL K+F   L +  +L++IP+GNA +  D   +CQHG
Sbjct: 34  SDKVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITDLKLIPFGNAHVSDDLTVTCQHG 93

Query: 88  PDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPIDCYR 147
            +EC LNA+EAC I  +P+   H++FI CVE  T     N W  C           DCY 
Sbjct: 94  EEECKLNALEACAIRTWPNQRLHYKFIRCVETNT-----NAWESCVKKYGGEKAINDCYN 153

Query: 148 NGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVNDKPLQ 207
               K ++L                         YA +T  L P H++VPW+ +N +PL 
Sbjct: 154 GDLSKELIL------------------------GYANQTLSLKPEHKYVPWMTLNGEPLY 213

Query: 208 EDYQNFMAYVCKAFNGSPA-PDACRSIMLDDR 239
           E+  +F+  VCKA+ G  A P  C S +L  R
Sbjct: 214 ENIGDFVDLVCKAYKGKAALPKLCYSSVLSQR 216

BLAST of Carg13300 vs. TAIR 10
Match: AT4G12870.1 (Gamma interferon responsive lysosomal thiol (GILT) reductase family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-33
Identity = 74/212 (34.91%), Postives = 110/212 (51.89%), Query Frame = 0

Query: 28  SGKVTVSVYYEALCPFCANFIVYHLVKLFENGLISAVNLRMIPWGNAWIQPDGAFSCQHG 87
           S KV +++YYE+LCP C +FIV  LVK+F++ L +  +++++P+G A +  +    CQHG
Sbjct: 30  SDKVELNLYYESLCPGCQSFIVDELVKVFDSDLDTITDVKLVPFGYAKVSNNLTVICQHG 89

Query: 88  PDECMLNAIEACTISIYPDTEKHFRFIHCVEGFTVQNRHNEWTKCFDIAQLSAIPIDCYR 147
            +EC LNA+EAC I+  P+ +  ++FI CVE     N  N  + C           DCY 
Sbjct: 90  EEECKLNALEACVINTLPNPKSQYKFIRCVE----NNTDNWESSCLKGYGNEKAINDCYN 149

Query: 148 NGHGKLVMLLSSSPSSLCFLTLIMEAVEMQLEQSYAYETTQLNPPHRFVPWVIVNDKPLQ 207
           +   K ++L                         YA +T+ L P H FVPWV +N KPL 
Sbjct: 150 SDLSKKLIL------------------------GYAKQTSSLKPKHEFVPWVTINSKPLY 209

Query: 208 EDYQNFMAYVCKAFNG-SPAPDACRSIMLDDR 239
               + +  VCKA+ G +P P  C S  L +R
Sbjct: 210 TKLDDLVGQVCKAYKGKTPLPIDCSSAALSER 213