Carg13270 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13270
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncell wall protein DAN4-like
LocationCarg_Chr07: 5977130 .. 5980799 (+)
RNA-Seq ExpressionCarg13270
SyntenyCarg13270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCGAAGCCGCGGAGTGCTGAGATAGTGAAATACTGCAAATACTTTGACAAACAGAGAGAATCCAAAGCAAACAAGATTCACTTCATCTCTCCATTCCCGTTTTCTCGATCTTCACTGCTCCACGCCGACGCAATCTTCCGATTCAAGCCCCACCGGAGCATCACTGGCGCTGATAAATGGTAATCCTTATTATTTGATTGCTATGATTCATGTTAAGTAATTAGTATATGCTTGTGGTTAAGCTTCTTGTATTCGGTTTGTTGATTTTTCGTTGGATCTGTTGGTCTCGTCAATGTTGAGGAGAAGACTGAGGACTGAGGATTGAGTTTTTTATACCTGCGGTTTTGTGTTGTTCTTCTTAACGGAAGATTTATGATCAATACTCAGATTCGGACCTGAGATTGAGAGAAAGGACCGGCGGAATCGTGCTTCGTTTCTTTTAGTTAGAATTTCTGGAATGTTCACGAACGGGACGGAGGGAGAGCTTGCAATGTTGCTCGATATGCGAACGATTGAGAAGGAGATGGATAGTCAGACTCTGCTACATCGGAGCTATTCGGAGGATCTTGATCCTAATTCAGAAACCGTTTCGTTTTCCAGTTCCGGTCCGTCATTTTTTCTTCCTACACTTTTTAGAAATTTCCAAGTTTATTATCGATCAGCTTTTTTAGAAGATTTTGACTCATTCTTTTTTTGGCCTTTTCTGATTCGTAAACTGCAGAACCTAACAATAATGGTGTTTGTTCACCCGTCTCCAAGAATGTTGTATCACCCGTTCCACCGCGGAAGATCAGAGCGGACGATTTTATGGATTCAGAAAATGAAAAATCTGAGTACGAATGGTAAGTAAATCACATTTTCTCTGATTTCATACTTGTAGCAACTCATATCTCAGTGATGAATCGGCTTTTCTTTTCAATTTGCTGTACATGAATTTTGAATACGAACCATGAAATATCCAATTTAAGAGTAGCATTGACTGGTATAGTGGGGTCGAATGTTGTTCATCAACGGATGCTTTTTCAAATAATTTCAAGAATTCATGTAGCACTAGGATGTAAACAGTACCTCACTGAGTTTTCTGGTCCTACTCGAACCTAAACAACGCGCCTGGTTCTTGTTTTCTTCATCTACATGGGGTTCCAATACGTCGTCTTTGTCAGCGAGATTCCCTTTTATTTTACCCGTTAAAGCGGAATGATATATTTGAAAAGACAAAAGACGACTCGTGAAAGTCATTTTGTTCCTCAACGTTATCATTCATTCTCCTTTCTTCCTTTATCGTAGAATAAAACAAAGCTCTGTTATACTGTGTACTTCCCCCTTCCCTTTAACTTTATTCAAAGGTTTTCACAAAATCGATTTCATCAACCGCTTTGTTTTTTTAAAAAAAACCTTGAACGTTTTCAAATCAAGAAAGTGATATTTGAATTTCTTCGCTATTTTCATTGAATCCCTTGTTTTGGGAGTTTTATTTTTAGATTATTAGCACCCCAACCTGCCTCAACTTTCTCCTTTATATCCATATTGATATCATCATTCAAGGACCAAGGAACAAGGACCATCCCCTGCCCTGAAACACTTCAGTAGCAATTTTTGAATGTCTTAGAACATGAATAGAGCCATGTAATGATGTTGTTGTAGATGTGGTAAATATTTGGTTATTATTGTTGATATGGGCATAATTATTATAAATTTGGATGCTGATCCATTATTTGAGTTCATACTTTTAAAAAGTGTTACTTTGTTATCAAATTCACTATTCATTGTACTCTCTTTTGTTTCTCAGGCTCCTTACACCACCTGGCACCCCACTGTTTTCATCAATGGAGTCGGAATCACAGAAAAATCCTACAAGTAAGAATGATATGCTGAATTCTCGTTCCACTGCTCTGAAACCGAGGGTAAGAGCTCTCAACAAGTTTGATTAACCGTTTGAATAACTGCAAATTGTATACTTTCCAATCAAAACTGTTGAATTTGCAATTATTCAAACTCAATGTTCCCAATCTGTATTTGATTAGCCTTACACCTTTTGGGGTTGCAGCTTGTAAACATCCAGGAAGAGTCTAACTCGGTGAGTAACATAGCGAATTTGCAATCTGGACAACTGAATTCTTCATGTGCTAGCAATAGAAAGCCCTCATCAAAGGCTTCTGCAGCTACTGCTTCCAGGTCTGCGACACCAACTTCACGGCCTACTTTAACTAAAAGTACAAAGCCTACAAGATCAGCCACGCCAACGTCACGAGTCACTGTCAAAGCTTCAGTCCCTCCGGCGAGATCTTCGACAACAGTGAAAACCACTGCTCGGTCATCAACACCATCTGAAAGATCCGTGTCGACTACAAAGAAAATATCAAGGTCAGCAACGCCTAATCGTTGTCCATCAAATCCAACATGTTCGTCTATCACTTCACGTCCTAATGAACGATCATCTTCGACCTCGAAGTTTAATGCTAGAAGCTCTAGTAATCCAAGACCTTCGAGGGGCACCTTTCCATTGATTATATCCAGACCTTCAAAACCCTCTGAGGTGCAAAATTTCCCACTTGATGAAGCTGCACGTCCATTATCAGAAAGGCCAGCTTCAGCCACAAGGGGAAGGCCAGTTGCAGCCAGTAGTGCTAAATCATCATCCCTTAGGGCTATGTCAAATGGGAAAACTCGGCAGAAACCAAGCTCTCCTTCGAAGCAACTTGCCTCAAACAGAAGTAGTGCATACAACAGTGGTAAAGTATTTCCATTCAAGCAGAAAATACGTTCCTCTGAAGAAAATGAGGTCAGCCCGGTGATCATGGGAACAAAGATGGTTGAAAGGGTAGTAAACATGAGGAAGCTCGCCCCTCCGAAGCAAGGCAGCTACCGCTCGAGCAGTGGTGATCCTTCCAGCAAGTCGTCTATTGACGCCACTGGCTTTGGAAGGACACTTTCTAACAAATCACTAGATATGGCTTTGAGGCACATGGTATGCCTCTCCTCTCTTCCATGATGGTTAATTACTACCATAGATAAACATGATCTTTGTTCAACATAACATGGCAGCACCTGATGTGGACTTTTCTTATTTTACAGGATATAACACTTAGCATTTCTGGTAAAGTACGTCCAGTGGTTACAAAAACTCAGGCCTCCTCCATCGACAGTGGCAGTTCAAGGTCTGCAAAAGTCGGAACAATTGGAGTTTCAGACTCTCCCCTTGCTACAAGCAGCAATGGCAGTTCTGCTCCGAGCGCATGGGGTAGTTCCATTCGTCTGGACGGCAGTGAAACTGAAGATACCGACGTTGCCACCGAGACATTGTCGTCATAGGGAATCCCTCTCCTGGCGAGCCATGTAGGCAACATTACCAGGTTCGTTCTCGGCTGCAAGTTTTTATAGCAGTGTATAACTCATGGATCCACTCTAAGGGAGGAGGTTGTAGGGAAGCAAATACATATTAGCGATGTAAAATCTATCATTAGTGTTAGAATTTATAGTAATTGTCCGATAATTCTATGAGTTATCAGAGTGTTGTGAAGAAAAAGAAATTGTATTTTAAGATTTGGAATTTGAAGAGCTCCTTCATATGAAATTTGTTGCTGTGTTGCGTAGGAATCTCCATGTTATCAATTATGGCTTCAGTTACCCAACCAATTAACTTTCTGTTAATAATTTTGTTTTTA

mRNA sequence

ATGGGGCGAAGCCGCGGAGTGCTGAGATAATTCGGACCTGAGATTGAGAGAAAGGACCGGCGGAATCGTGCTTCGTTTCTTTTAGTTAGAATTTCTGGAATGTTCACGAACGGGACGGAGGGAGAGCTTGCAATGTTGCTCGATATGCGAACGATTGAGAAGGAGATGGATAGTCAGACTCTGCTACATCGGAGCTATTCGGAGGATCTTGATCCTAATTCAGAAACCGTTTCGTTTTCCAGTTCCGGTCCGTCATTTTTTCTTCCTACACTTTTTAGAAATTTCCAAGTTTATTATCGATCAGCTTTTTTAGAAGATTTTGACTCATTCTTTTTTTGGCCTTTTCTGATTCGTAAACTGCAGAACCTAACAATAATGGTGTTTGTTCACCCGTCTCCAAGAATGTTGTATCACCCGTTCCACCGCGGAAGATCAGAGCGGACGATTTTATGGATTCAGAAAATGAAAAATCTGAGTACGAATGGTAAGCTCCTTACACCACCTGGCACCCCACTGTTTTCATCAATGGAGTCGGAATCACAGAAAAATCCTACAAGTAAGAATGATATGCTGAATTCTCGTTCCACTGCTCTGAAACCGAGGCTTGTAAACATCCAGGAAGAGTCTAACTCGGTGAGTAACATAGCGAATTTGCAATCTGGACAACTGAATTCTTCATGTGCTAGCAATAGAAAGCCCTCATCAAAGGCTTCTGCAGCTACTGCTTCCAGGTCTGCGACACCAACTTCACGGCCTACTTTAACTAAAAGTACAAAGCCTACAAGATCAGCCACGCCAACGTCACGAGTCACTGTCAAAGCTTCAGTCCCTCCGGCGAGATCTTCGACAACAGTGAAAACCACTGCTCGGTCATCAACACCATCTGAAAGATCCGTGTCGACTACAAAGAAAATATCAAGGTCAGCAACGCCTAATCGTTGTCCATCAAATCCAACATGTTCGTCTATCACTTCACGTCCTAATGAACGATCATCTTCGACCTCGAAGTTTAATGCTAGAAGCTCTAGTAATCCAAGACCTTCGAGGGGCACCTTTCCATTGATTATATCCAGACCTTCAAAACCCTCTGAGGTGCAAAATTTCCCACTTGATGAAGCTGCACGTCCATTATCAGAAAGGCCAGCTTCAGCCACAAGGGGAAGGCCAGTTGCAGCCAGTAGTGCTAAATCATCATCCCTTAGGGCTATGTCAAATGGGAAAACTCGGCAGAAACCAAGCTCTCCTTCGAAGCAACTTGCCTCAAACAGAAGTAGTGCATACAACAGTGGTAAAGTATTTCCATTCAAGCAGAAAATACGTTCCTCTGAAGAAAATGAGGTCAGCCCGGTGATCATGGGAACAAAGATGGTTGAAAGGGTAGTAAACATGAGGAAGCTCGCCCCTCCGAAGCAAGGCAGCTACCGCTCGAGCAGTGGTGATCCTTCCAGCAAGTCGTCTATTGACGCCACTGGCTTTGGAAGGACACTTTCTAACAAATCACTAGATATGGCTTTGAGGCACATGGATATAACACTTAGCATTTCTGGTAAAGTACGTCCAGTGGTTACAAAAACTCAGGCCTCCTCCATCGACAGTGGCAGTTCAAGGTCTGCAAAAGTCGGAACAATTGGAGTTTCAGACTCTCCCCTTGCTACAAGCAGCAATGGCAGTTCTGCTCCGAGCGCATGGGGTAGTTCCATTCGTCTGGACGGCAGTGAAACTGAAGATACCGACGTTGCCACCGAGACATTGTCGTCATAGGGAATCCCTCTCCTGGCGAGCCATGTAGGCAACATTACCAGGTTCGTTCTCGGCTGCAAGTTTTTATAGCAGTGTATAACTCATGGATCCACTCTAAGGGAGGAGGTTGTAGGGAAGCAAATACATATTAGCGATGTAAAATCTATCATTAGTGTTAGAATTTATAGTAATTGTCCGATAATTCTATGAGTTATCAGAGTGTTGTGAAGAAAAAGAAATTGTATTTTAAGATTTGGAATTTGAAGAGCTCCTTCATATGAAATTTGTTGCTGTGTTGCGTAGGAATCTCCATGTTATCAATTATGGCTTCAGTTACCCAACCAATTAACTTTCTGTTAATAATTTTGTTTTTA

Coding sequence (CDS)

ATGTTCACGAACGGGACGGAGGGAGAGCTTGCAATGTTGCTCGATATGCGAACGATTGAGAAGGAGATGGATAGTCAGACTCTGCTACATCGGAGCTATTCGGAGGATCTTGATCCTAATTCAGAAACCGTTTCGTTTTCCAGTTCCGGTCCGTCATTTTTTCTTCCTACACTTTTTAGAAATTTCCAAGTTTATTATCGATCAGCTTTTTTAGAAGATTTTGACTCATTCTTTTTTTGGCCTTTTCTGATTCGTAAACTGCAGAACCTAACAATAATGGTGTTTGTTCACCCGTCTCCAAGAATGTTGTATCACCCGTTCCACCGCGGAAGATCAGAGCGGACGATTTTATGGATTCAGAAAATGAAAAATCTGAGTACGAATGGTAAGCTCCTTACACCACCTGGCACCCCACTGTTTTCATCAATGGAGTCGGAATCACAGAAAAATCCTACAAGTAAGAATGATATGCTGAATTCTCGTTCCACTGCTCTGAAACCGAGGCTTGTAAACATCCAGGAAGAGTCTAACTCGGTGAGTAACATAGCGAATTTGCAATCTGGACAACTGAATTCTTCATGTGCTAGCAATAGAAAGCCCTCATCAAAGGCTTCTGCAGCTACTGCTTCCAGGTCTGCGACACCAACTTCACGGCCTACTTTAACTAAAAGTACAAAGCCTACAAGATCAGCCACGCCAACGTCACGAGTCACTGTCAAAGCTTCAGTCCCTCCGGCGAGATCTTCGACAACAGTGAAAACCACTGCTCGGTCATCAACACCATCTGAAAGATCCGTGTCGACTACAAAGAAAATATCAAGGTCAGCAACGCCTAATCGTTGTCCATCAAATCCAACATGTTCGTCTATCACTTCACGTCCTAATGAACGATCATCTTCGACCTCGAAGTTTAATGCTAGAAGCTCTAGTAATCCAAGACCTTCGAGGGGCACCTTTCCATTGATTATATCCAGACCTTCAAAACCCTCTGAGGTGCAAAATTTCCCACTTGATGAAGCTGCACGTCCATTATCAGAAAGGCCAGCTTCAGCCACAAGGGGAAGGCCAGTTGCAGCCAGTAGTGCTAAATCATCATCCCTTAGGGCTATGTCAAATGGGAAAACTCGGCAGAAACCAAGCTCTCCTTCGAAGCAACTTGCCTCAAACAGAAGTAGTGCATACAACAGTGGTAAAGTATTTCCATTCAAGCAGAAAATACGTTCCTCTGAAGAAAATGAGGTCAGCCCGGTGATCATGGGAACAAAGATGGTTGAAAGGGTAGTAAACATGAGGAAGCTCGCCCCTCCGAAGCAAGGCAGCTACCGCTCGAGCAGTGGTGATCCTTCCAGCAAGTCGTCTATTGACGCCACTGGCTTTGGAAGGACACTTTCTAACAAATCACTAGATATGGCTTTGAGGCACATGGATATAACACTTAGCATTTCTGGTAAAGTACGTCCAGTGGTTACAAAAACTCAGGCCTCCTCCATCGACAGTGGCAGTTCAAGGTCTGCAAAAGTCGGAACAATTGGAGTTTCAGACTCTCCCCTTGCTACAAGCAGCAATGGCAGTTCTGCTCCGAGCGCATGGGGTAGTTCCATTCGTCTGGACGGCAGTGAAACTGAAGATACCGACGTTGCCACCGAGACATTGTCGTCATAG

Protein sequence

MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFRNFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQKMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVSNIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVKASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAASSAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLSISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSETEDTDVATETLSS
Homology
BLAST of Carg13270 vs. NCBI nr
Match: KAG7027390.1 (hypothetical protein SDJN02_11402 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 995.3 bits (2572), Expect = 2.0e-286
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
           NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ
Sbjct: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
           KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK
Sbjct: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540

Query: 541 TEDTDVATETLSS 554
           TEDTDVATETLSS
Sbjct: 541 TEDTDVATETLSS 553

BLAST of Carg13270 vs. NCBI nr
Match: KAG6595382.1 (hypothetical protein SDJN03_11935, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 800.0 bits (2065), Expect = 1.2e-227
Identity = 477/553 (86.26%), Postives = 483/553 (87.34%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSS P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK+                          R   ++ 
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKI--------------------------RADDFMD 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 SENEKSEYEWLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK
Sbjct: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSS+ENEVSPVIMG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSDENEVSPVIMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 513

Query: 541 TEDTDVATETLSS 554
           TEDTDVATETLSS
Sbjct: 541 TEDTDVATETLSS 513

BLAST of Carg13270 vs. NCBI nr
Match: XP_022966648.1 (TRIO and F-actin-binding protein-like [Cucurbita maxima])

HSP 1 Score: 776.5 bits (2004), Expect = 1.5e-220
Identity = 464/553 (83.91%), Postives = 476/553 (86.08%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSS P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKIR---------------AEDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESESQK PTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 -----SEYEWLLTPPGTPLFSSMESESQKTPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIAN QSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRV VK
Sbjct: 181 NIANFQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVNVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPP RSSTT KTTARSSTPSERSVS+TKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPVRSSTTAKTTARSSTPSERSVSSTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFP I++RPSKPSEV NFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPSIMTRPSKPSEVLNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQ+IRSS+ENEVSPV+MG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQRIRSSDENEVSPVVMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSID TGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDVTGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKV TIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVRTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 513

Query: 541 TEDTDVATETLSS 554
           TEDTDVATETLSS
Sbjct: 541 TEDTDVATETLSS 513

BLAST of Carg13270 vs. NCBI nr
Match: XP_022931827.1 (TRIO and F-actin-binding protein-like [Cucurbita moschata])

HSP 1 Score: 776.2 bits (2003), Expect = 1.9e-220
Identity = 463/553 (83.73%), Postives = 477/553 (86.26%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSS P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKIR---------------AEDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESES+KNPTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 -----SEYEWLLTPPGTPLFSSMESESEKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPT RV VK
Sbjct: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTLRVNVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPP R STT KTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPVRPSTTAKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFPLI++RPSKPSEV NFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPLIMTRPSKPSEVLNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFK +IRSS+ENEVSPV+MG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKPRIRSSDENEVSPVVMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSA SAWGSSIRL+GSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAQSAWGSSIRLEGSE 513

Query: 541 TEDTDVATETLSS 554
           TEDTD+ATETLSS
Sbjct: 541 TEDTDIATETLSS 513

BLAST of Carg13270 vs. NCBI nr
Match: XP_023517474.1 (TRIO and F-actin-binding protein-like [Cucurbita pepo subsp. pepo] >XP_023517475.1 TRIO and F-actin-binding protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.0 bits (2000), Expect = 4.3e-220
Identity = 465/554 (83.94%), Postives = 478/554 (86.28%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSS+ P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSAEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKIR---------------AEDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 -----SEYEWLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIANLQSGQLNSSCASNRK SSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRV VK
Sbjct: 181 NIANLQSGQLNSSCASNRKASSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVNVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPP RSSTT KTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPVRSSTTAKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFP I++RPSKPSEV NFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPSIMTRPSKPSEVLNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFK +IRSS+ENEVSPV+MG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKPRIRSSDENEVSPVVMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSK-SSIDATGFGRTLSNKSLDMALRHMDITL 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSK SSIDATGFGRTLSNKSLDMALRHMDITL
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSSIDATGFGRTLSNKSLDMALRHMDITL 480

Query: 481 SISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGS 540
           SISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGS
Sbjct: 481 SISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGS 514

Query: 541 ETEDTDVATETLSS 554
           ETEDTD+ATETLSS
Sbjct: 541 ETEDTDIATETLSS 514

BLAST of Carg13270 vs. ExPASy TrEMBL
Match: A0A6J1HSQ1 (TRIO and F-actin-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC111466271 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 7.2e-221
Identity = 464/553 (83.91%), Postives = 476/553 (86.08%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSS P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKIR---------------AEDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESESQK PTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 -----SEYEWLLTPPGTPLFSSMESESQKTPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIAN QSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRV VK
Sbjct: 181 NIANFQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVNVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPP RSSTT KTTARSSTPSERSVS+TKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPVRSSTTAKTTARSSTPSERSVSSTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFP I++RPSKPSEV NFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPSIMTRPSKPSEVLNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQ+IRSS+ENEVSPV+MG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQRIRSSDENEVSPVVMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSID TGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDVTGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKV TIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVRTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 513

Query: 541 TEDTDVATETLSS 554
           TEDTDVATETLSS
Sbjct: 541 TEDTDVATETLSS 513

BLAST of Carg13270 vs. ExPASy TrEMBL
Match: A0A6J1EUT5 (TRIO and F-actin-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111438122 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 9.4e-221
Identity = 463/553 (83.73%), Postives = 477/553 (86.26%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSS P+        
Sbjct: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSEPN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGVCSPVSKNVVSPVPPRKIR---------------AEDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLFSSMESES+KNPTSKNDMLNSRSTALKPRLVNIQEESNSVS
Sbjct: 121 -----SEYEWLLTPPGTPLFSSMESESEKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180

Query: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVK 240
           NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPT RV VK
Sbjct: 181 NIANLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTLRVNVK 240

Query: 241 ASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300
           ASVPP R STT KTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS
Sbjct: 241 ASVPPVRPSTTAKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSS 300

Query: 301 TSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAAS 360
           TSKFNARSSSNPRPSRGTFPLI++RPSKPSEV NFPLDEAARPLSERPASATRGRPVAAS
Sbjct: 301 TSKFNARSSSNPRPSRGTFPLIMTRPSKPSEVLNFPLDEAARPLSERPASATRGRPVAAS 360

Query: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMG 420
           SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFK +IRSS+ENEVSPV+MG
Sbjct: 361 SAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKPRIRSSDENEVSPVVMG 420

Query: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480
           TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS
Sbjct: 421 TKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLS 480

Query: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRLDGSE 540
           ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSA SAWGSSIRL+GSE
Sbjct: 481 ISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAQSAWGSSIRLEGSE 513

Query: 541 TEDTDVATETLSS 554
           TEDTD+ATETLSS
Sbjct: 541 TEDTDIATETLSS 513

BLAST of Carg13270 vs. ExPASy TrEMBL
Match: A0A0A0KXX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G308580 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.6e-180
Identity = 391/557 (70.20%), Postives = 429/557 (77.02%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTN TEGE+ MLLDMRT+EKEMD+  LLHRS SEDLDPN ETVSFS S  +        
Sbjct: 1   MFTNDTEGEVVMLLDMRTVEKEMDNDNLLHRSCSEDLDPNLETVSFSLSEAN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK+                          RT  ++ 
Sbjct: 61  ------NNGGCSPVSKNVVSPVPPRKI--------------------------RTEDFLD 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLF SME+ESQKN T+KNDM+NSRSTALKPRLVNIQEE NSVS
Sbjct: 121 SENEKSDYDWLLTPPGTPLFPSMETESQKNTTNKNDMMNSRSTALKPRLVNIQEECNSVS 180

Query: 181 NIA----NLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSR 240
           NIA    NLQSGQLNSSCASN+KPSSKAS+ATASRSATPTSR TLTK+TKP+RSATPTSR
Sbjct: 181 NIASKHPNLQSGQLNSSCASNKKPSSKASSATASRSATPTSRQTLTKTTKPSRSATPTSR 240

Query: 241 VTVKASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNE 300
           V  KAS PP RSST  KTTA+SSTP+E+SV+TTK+ SRSATPNRCPS PTCSSITSRPN 
Sbjct: 241 VNTKASAPPVRSSTPAKTTAQSSTPTEKSVTTTKQTSRSATPNRCPSKPTCSSITSRPNG 300

Query: 301 RSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRP 360
           RSSSTSK NARSSSNPRPSRGTFP I +RPSKPSE   F LDEAA P+ ERPAS T+GRP
Sbjct: 301 RSSSTSKSNARSSSNPRPSRGTFPSIKTRPSKPSEAPKFTLDEAASPMPERPASTTKGRP 360

Query: 361 VAASSAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSP 420
           + ASSAKSSS R MSNGKTRQKP+SPSKQ ASN SSAYNSGKVFPFK +IR  ++NEVSP
Sbjct: 361 IVASSAKSSSGRTMSNGKTRQKPNSPSKQFASNGSSAYNSGKVFPFKPRIRCPDDNEVSP 420

Query: 421 VIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMD 480
           V+MGTKMVERVVNMRKL PPKQG YR S GDPSSKSS D  GFGRTLSNKSLDMALRHMD
Sbjct: 421 VVMGTKMVERVVNMRKLVPPKQGDYRPSIGDPSSKSSADIPGFGRTLSNKSLDMALRHMD 480

Query: 481 ITLSISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRL 540
           IT SISGKVRPV+TKTQ SS+++GSSRS K GT GVSDSPLATSSNGSSAPSAW +SIRL
Sbjct: 481 ITRSISGKVRPVITKTQTSSMNNGSSRSTKAGTTGVSDSPLATSSNGSSAPSAWSNSIRL 517

Query: 541 DGSETEDTDVATETLSS 554
           D SETED +++TE +SS
Sbjct: 541 DESETEDNELSTEMVSS 517

BLAST of Carg13270 vs. ExPASy TrEMBL
Match: A0A6J1DET0 (probable serine/threonine-protein kinase nek3 OS=Momordica charantia OX=3673 GN=LOC111019413 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.5e-175
Identity = 398/564 (70.57%), Postives = 434/564 (76.95%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MF N TEGELAMLLD+RT+EKE+DS  LLHRSYSEDLDPNSE VSFS S           
Sbjct: 1   MFGNATEGELAMLLDLRTVEKEIDSDNLLHRSYSEDLDPNSEIVSFSCS----------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                                    +L N         SP          R  RT  ++ 
Sbjct: 61  -------------------------ELNNNG-----GCSPISKNASSVPPRKTRTEDFMD 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLF SME+ESQKN T+KND  NSRSTALKPRLVNIQEES+SVS
Sbjct: 121 SENEKSDYDWLLTPPGTPLFPSMETESQKNSTNKNDTPNSRSTALKPRLVNIQEESDSVS 180

Query: 181 NIA-----NLQSG-QLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPT 240
           NIA     NLQSG QLNSSCAS+++PSSKASAATASRSATPTSRPTL+KSTKP+RSATPT
Sbjct: 181 NIASKHHPNLQSGQQLNSSCASDKRPSSKASAATASRSATPTSRPTLSKSTKPSRSATPT 240

Query: 241 SRV---TVKASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSIT 300
           SRV   +VKA+VPPARSST  K TAR STP ERSVS +K+ SRSATPNRCPS PTC SI 
Sbjct: 241 SRVSLASVKAAVPPARSSTPGKATARLSTPIERSVSASKQTSRSATPNRCPSKPTCPSIV 300

Query: 301 SRPNERSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASA 360
           SRP  RSSSTSKFNARSSSNPRPSRGTFP I  RPSKPSEV NFPLD AA PL ERPASA
Sbjct: 301 SRPTGRSSSTSKFNARSSSNPRPSRGTFPSIKPRPSKPSEVPNFPLDAAAHPLPERPASA 360

Query: 361 TRGRPVAA-SSAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSE 420
           T+GRPVAA SSAK SS R MSNGKTRQKPSSPSKQLASN +    SG +FPFKQ+IRSS+
Sbjct: 361 TKGRPVAASSSAKPSSGRTMSNGKTRQKPSSPSKQLASNGT----SGTIFPFKQRIRSSD 420

Query: 421 ENEVSPVIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDM 480
           +NEVSPV+MGTKMVERVVNMRKLAPPKQG +RSS+GDP+SK S D +GFGRTLSNKSLDM
Sbjct: 421 DNEVSPVVMGTKMVERVVNMRKLAPPKQGDHRSSNGDPASKPSADTSGFGRTLSNKSLDM 480

Query: 481 ALRHMDITLSISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAW 540
           ALRHMDIT S+SGKVRPVVTKTQASS+D+ +SRS K GTIGV+DSPLATSSNGSSAPSAW
Sbjct: 481 ALRHMDITRSLSGKVRPVVTKTQASSMDNANSRSIKFGTIGVADSPLATSSNGSSAPSAW 519

Query: 541 GSSIRLDGSETEDT-DVATETLSS 554
            SSIRLDGSETED  D+A ET+SS
Sbjct: 541 SSSIRLDGSETEDNDDLAVETMSS 519

BLAST of Carg13270 vs. ExPASy TrEMBL
Match: A0A5D3BCG1 (Cell wall protein DAN4-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00890 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.9e-174
Identity = 383/557 (68.76%), Postives = 426/557 (76.48%), Query Frame = 0

Query: 1   MFTNGTEGELAMLLDMRTIEKEMDSQTLLHRSYSEDLDPNSETVSFSSSGPSFFLPTLFR 60
           MFTN +EGE+ MLLDMRT+EKEMD+  LLHRS S   DPN ETVSFS S  +        
Sbjct: 1   MFTNDSEGEVVMLLDMRTVEKEMDNDNLLHRSCSG--DPNLETVSFSVSEAN-------- 60

Query: 61  NFQVYYRSAFLEDFDSFFFWPFLIRKLQNLTIMVFVHPSPRMLYHPFHRGRSERTILWIQ 120
                  +            P   RK++                  F    +E+      
Sbjct: 61  ------NNGGCSPVSKNAVSPVPPRKIKT---------------EDFMDSENEK------ 120

Query: 121 KMKNLSTNGKLLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVS 180
                S    LLTPPGTPLF SME+ESQKN T+KNDM+ SRSTALKPRLVNIQEE NSVS
Sbjct: 121 -----SDYDWLLTPPGTPLFPSMETESQKNATNKNDMMISRSTALKPRLVNIQEECNSVS 180

Query: 181 NIA----NLQSGQLNSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSR 240
           NIA    NLQSGQLNS+CA+N+KPSSKAS+ATASRSATPTSRPTL+K+TKP+RSATPTSR
Sbjct: 181 NIASKHPNLQSGQLNSACATNKKPSSKASSATASRSATPTSRPTLSKTTKPSRSATPTSR 240

Query: 241 VTVKASVPPARSSTTVKTTARSSTPSERSVSTTKKISRSATPNRCPSNPTCSSITSRPNE 300
           V  KAS PP RSST  KTTA+SSTP+E+SVSTTK+ SRSATPNRCPS PTCSSI SRPN 
Sbjct: 241 VNTKASAPPVRSSTPAKTTAQSSTPTEKSVSTTKQTSRSATPNRCPSKPTCSSIASRPNG 300

Query: 301 RSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLDEAARPLSERPASATRGRP 360
           RSSSTSK NARSSSNPRPSR TFP I +RPSKPSE  NFPLDEAA  + ERP S T+GRP
Sbjct: 301 RSSSTSKSNARSSSNPRPSRSTFPSIKTRPSKPSEAPNFPLDEAASSMPERPVSTTKGRP 360

Query: 361 VAASSAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSP 420
           + ASS+KSSS R MSNGKTRQKPSSPSKQLASN SSAYNSGKVFPFK +IRS+++NEVSP
Sbjct: 361 IVASSSKSSSGRTMSNGKTRQKPSSPSKQLASNGSSAYNSGKVFPFKPRIRSADDNEVSP 420

Query: 421 VIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMD 480
           V+MGTKMVERVVNMRKLAPPKQG YR S GDPSSKSS+D  GFGRTLSNKSLDMALRHMD
Sbjct: 421 VVMGTKMVERVVNMRKLAPPKQGDYRPSIGDPSSKSSVDIPGFGRTLSNKSLDMALRHMD 480

Query: 481 ITLSISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSPLATSSNGSSAPSAWGSSIRL 540
           IT SISGKVR V+TKT  SS+++GSSRS K GT GVSDSPLATSSNGSSAPSAW SSI L
Sbjct: 481 ITRSISGKVRSVITKTHTSSMNNGSSRSTKAGTTGVSDSPLATSSNGSSAPSAWSSSIHL 515

Query: 541 DGSETEDTDVATETLSS 554
           D SE ED +++TE +SS
Sbjct: 541 DDSEIEDNELSTEMVSS 515

BLAST of Carg13270 vs. TAIR 10
Match: AT2G40070.1 (BEST Arabidopsis thaliana protein match is: proline-rich family protein (TAIR:AT3G09000.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 185.7 bits (470), Expect = 1.0e-46
Identity = 198/497 (39.84%), Postives = 259/497 (52.11%), Query Frame = 0

Query: 131 LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVSNIANLQ---S 190
           LLTPPGTPLF S+E ES +   S+     SR   L  RL N   ES + +++ + Q   S
Sbjct: 104 LLTPPGTPLFPSLEMESHRTMMSQTGDSKSRPATLTSRLANSSTESAARNHLTSRQQTSS 163

Query: 191 GQLNSSCASNRKPSSKASAATASRSATPTSR-PTLTKSTKPTRSATPTSRVTVKA----- 250
             L+SS  ++R+PSS  S    SR ATPT R  TLT ++K +R +TPTSR TV +     
Sbjct: 164 PGLSSSSGASRRPSS--SGGPGSRPATPTGRSSTLTANSKSSRPSTPTSRATVSSATRPS 223

Query: 251 --------------------------------------------------------SVPP 310
                                                                   S  P
Sbjct: 224 LTNSRSTVSATTKPTPMSRSTSLSSSRLTPTASKPTTSTARSAGSVTRSTPSTTTKSAGP 283

Query: 311 ARSSTTV-KTTARSSTPSER-SVSTTKKISRSATPNRCP----------SNPTCSSITSR 370
           +RS+T + ++TARSSTP+ R ++  +K ISRS+TP R P          +NPT S I  +
Sbjct: 284 SRSTTPLSRSTARSSTPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQI--K 343

Query: 371 PNERSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLD---EAARPLSERPAS 430
           P+  S + +K     S NP  SR   P + SRP KPS++  F L+        L ERP S
Sbjct: 344 PS--SPAPAKPMPTPSKNPALSRAASPTVRSRPWKPSDMPGFSLETPPNLRTTLPERPLS 403

Query: 431 ATRGRPVAASSAKSS-SLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSS 490
           ATRGRP A SS   S        G+ R++  SPS+     R+  Y+SG   P   +  S 
Sbjct: 404 ATRGRPGAPSSRSGSVEPGGPPGGRPRRQSCSPSR----GRAPMYSSGSSVPAVNRGYSK 463

Query: 491 EENEVSPVIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSI-DATGFGRTLSNKSL 544
             + VSPV+MGTKMVERV+NMRKLAPP+     S  G+ S+KSS  D+ GFGRTLS KSL
Sbjct: 464 ASDNVSPVMMGTKMVERVINMRKLAPPRSDDKGSPHGNLSAKSSSPDSAGFGRTLSKKSL 523

BLAST of Carg13270 vs. TAIR 10
Match: AT2G40070.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: proline-rich family protein (TAIR:AT3G09000.1); Has 108635 Blast hits to 60786 proteins in 2176 species: Archae - 287; Bacteria - 15142; Metazoa - 39415; Fungi - 26849; Plants - 4416; Viruses - 2864; Other Eukaryotes - 19662 (source: NCBI BLink). )

HSP 1 Score: 185.7 bits (470), Expect = 1.0e-46
Identity = 198/497 (39.84%), Postives = 259/497 (52.11%), Query Frame = 0

Query: 131 LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVSNIANLQ---S 190
           LLTPPGTPLF S+E ES +   S+     SR   L  RL N   ES + +++ + Q   S
Sbjct: 64  LLTPPGTPLFPSLEMESHRTMMSQTGDSKSRPATLTSRLANSSTESAARNHLTSRQQTSS 123

Query: 191 GQLNSSCASNRKPSSKASAATASRSATPTSR-PTLTKSTKPTRSATPTSRVTVKA----- 250
             L+SS  ++R+PSS  S    SR ATPT R  TLT ++K +R +TPTSR TV +     
Sbjct: 124 PGLSSSSGASRRPSS--SGGPGSRPATPTGRSSTLTANSKSSRPSTPTSRATVSSATRPS 183

Query: 251 --------------------------------------------------------SVPP 310
                                                                   S  P
Sbjct: 184 LTNSRSTVSATTKPTPMSRSTSLSSSRLTPTASKPTTSTARSAGSVTRSTPSTTTKSAGP 243

Query: 311 ARSSTTV-KTTARSSTPSER-SVSTTKKISRSATPNRCP----------SNPTCSSITSR 370
           +RS+T + ++TARSSTP+ R ++  +K ISRS+TP R P          +NPT S I  +
Sbjct: 244 SRSTTPLSRSTARSSTPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQI--K 303

Query: 371 PNERSSSTSKFNARSSSNPRPSRGTFPLIISRPSKPSEVQNFPLD---EAARPLSERPAS 430
           P+  S + +K     S NP  SR   P + SRP KPS++  F L+        L ERP S
Sbjct: 304 PS--SPAPAKPMPTPSKNPALSRAASPTVRSRPWKPSDMPGFSLETPPNLRTTLPERPLS 363

Query: 431 ATRGRPVAASSAKSS-SLRAMSNGKTRQKPSSPSKQLASNRSSAYNSGKVFPFKQKIRSS 490
           ATRGRP A SS   S        G+ R++  SPS+     R+  Y+SG   P   +  S 
Sbjct: 364 ATRGRPGAPSSRSGSVEPGGPPGGRPRRQSCSPSR----GRAPMYSSGSSVPAVNRGYSK 423

Query: 491 EENEVSPVIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSI-DATGFGRTLSNKSL 544
             + VSPV+MGTKMVERV+NMRKLAPP+     S  G+ S+KSS  D+ GFGRTLS KSL
Sbjct: 424 ASDNVSPVMMGTKMVERVINMRKLAPPRSDDKGSPHGNLSAKSSSPDSAGFGRTLSKKSL 483

BLAST of Carg13270 vs. TAIR 10
Match: AT3G09000.1 (proline-rich family protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.6e-42
Identity = 187/455 (41.10%), Postives = 252/455 (55.38%), Query Frame = 0

Query: 131 LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVSNIANLQSGQL 190
           LLTPPGTP F   E ES ++  +++D  NSR T LK RL N +E+  S +N     S   
Sbjct: 84  LLTPPGTPQF---EKESHRSVMNQHDAPNSRPTVLKSRLGNCREDIVSGNNNKPQTS--- 143

Query: 191 NSSCASNRKPSSKASAATASRSATPTSRPT--LTKSTKP-------TRSATPTSRVTVKA 250
           +SS A  R+PSS  S+ + SR ATPT R T   T +++P       +RS+TPTSR T+ A
Sbjct: 144 SSSVAGLRRPSSSGSSRSTSRPATPTRRSTTPTTSTSRPVTTRASNSRSSTPTSRATLTA 203

Query: 251 -----SVPPARSSTTVKTTARSSTPSE-----RSVSTTKKISRSATPNRCPSNPTCSSIT 310
                S    R++TT   +ARS+TP+       S S+ K +SR ATP R PS PT  SI 
Sbjct: 204 ARATTSTAAPRTTTTSSGSARSATPTRSNPRPSSASSKKPVSRPATPTRRPSTPTGPSIV 263

Query: 311 SRPNERSSSTSKFNARSSSNPRPSRGTFP---LIISRPSKPSEVQNFPLD---EAARPLS 370
           S     S  TS     +S +  PSRGT P   L  SRP KP E+  F L+        L+
Sbjct: 264 S-SKAPSRGTSPSPTVNSLSKAPSRGTSPSPTLNSSRPWKPPEMPGFSLEAPPNLRTTLA 323

Query: 371 ERPASATRGRPVAASS--AKSSSLR------AMSNGKTRQKPSSPSKQLAS-NRSSAYNS 430
           +RP SA+RGRP  AS+  ++S S+       +  +G  R++  SPS+  A    ++   +
Sbjct: 324 DRPVSASRGRPGVASAPGSRSGSIERGGGPTSGGSGNARRQSCSPSRGRAPIGNTNGSLT 383

Query: 431 GKVFPFKQKIRSSEENEVSPVIMGTKMVERVVNMRKLAPPKQGSYRSSSGDPSSKSSIDA 490
           G     K     S  + +SPV MG KMVERVVNMRKL PP+  +     G   S S+ ++
Sbjct: 384 GVRGRAKASNGGSGCDNLSPVAMGNKMVERVVNMRKLGPPRL-TENGGRGSGKSSSAFNS 443

Query: 491 TGFGRTLSNKSLDMALRHMDITLSISGKVRPVVTKTQASSIDSGSSRSAKVGTIGVSDSP 550
            G+GR LS  S+DMA+RHMDI   ++G +RP+VTK  ASS+ S  SR        VS SP
Sbjct: 444 LGYGRNLSKSSIDMAIRHMDIRRGMTGNLRPLVTKVPASSMYSVRSRPG-----SVSSSP 503

BLAST of Carg13270 vs. TAIR 10
Match: AT5G01280.1 (BEST Arabidopsis thaliana protein match is: proline-rich family protein (TAIR:AT3G09000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 73.2 bits (178), Expect = 7.5e-13
Identity = 138/460 (30.00%), Postives = 208/460 (45.22%), Query Frame = 0

Query: 131 LLTPPGTPLFSSMESESQKNPTSKNDMLNSRSTALKPRLVNIQEESNSVSNIANLQSGQL 190
           L+TPPG+P          +N T+  +  +     L  RL N  +E  S     +L S   
Sbjct: 29  LVTPPGSP---------SRNVTNHLNAPDDNLMTLISRLENYSKE-ESEHQTTSLHS--- 88

Query: 191 NSSCASNRKPSSKASAATASRSATPTSRPTLTKSTKPTRSATPTSRVTVKASVPPARSST 250
           +SS +  R+PSS +S+ + SR  TPT +      T   R +TPTSR T   +     SS+
Sbjct: 89  SSSVSGIRRPSSSSSSRSTSRPPTPTRK----SKTPAKRPSTPTSRATSTTTRATLTSSS 148

Query: 251 TVKTTARSSTPSERSVSTTKKI----SRSATPNRCPSNPTCSSITS-RPNER--SSSTSK 310
           T  +T   S PS  S + T ++    +R+  P       T  S TS R N R  S+  SK
Sbjct: 149 TTSSTRSWSRPSSSSGTGTSRVTLTAARATRPTTSTDQQTTGSATSTRSNNRPMSAPNSK 208

Query: 311 FNARSS------------------------SNPRPSRGTFPLIISRPSKPSEVQNFPLDE 370
             +RSS                        S P  S    P++ SRP +P E+  F ++ 
Sbjct: 209 PGSRSSTPTRRPSTPNGSSTVLRSKPTKPLSKPALSLEASPIVRSRPWEPYEMPGFSVEA 268

Query: 371 AAR---PLSERPASATRGRPVAASSAKSSSLRAMSNGKTRQKPSSPSKQLASNRSSAYNS 430
            +     L +RP +A+  R  A  ++ SS   +      +++  SPS+  A N +     
Sbjct: 269 PSNLRTTLPDRPQTASSSRTRAFDASSSSRSASTERDVAKRQSCSPSRSRAPNGNVNGAV 328

Query: 431 GKVFPFKQKIRSSEENEVSPVIMGTKMVERVVNMRKLAPPK---QGSYR--SSSGDPS-- 490
             +   + K  + +   +S    G + VE+VVNMRKLA P+    GS R     GD S  
Sbjct: 329 PSLRGQRAKTNNDDGRLISHAAKGNQKVEKVVNMRKLATPRLTESGSRRLGGGGGDSSAG 388

Query: 491 -SKSSIDATGFGRTLSNKSLDMALRHMDITL-SISGKVRPVVTKTQASSIDSGSSRSAKV 548
            S S     GFGR LS  S+DMALRHMD+   S++G  R  VTK  A+S+ S  S     
Sbjct: 389 KSSSGSGGFGFGRNLSKSSIDMALRHMDVRKGSMAGNFRHSVTKAPATSVYSVRS----- 448

BLAST of Carg13270 vs. TAIR 10
Match: AT2G38160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G40070.2); Has 972 Blast hits to 731 proteins in 211 species: Archae - 0; Bacteria - 236; Metazoa - 194; Fungi - 201; Plants - 218; Viruses - 32; Other Eukaryotes - 91 (source: NCBI BLink). )

HSP 1 Score: 70.9 bits (172), Expect = 3.7e-12
Identity = 83/245 (33.88%), Postives = 127/245 (51.84%), Query Frame = 0

Query: 263 ERSVSTTKKISRSATPNRCPSNPTCSSITSRPNERSSSTSKFNARSSSNP--RPSRGTFP 322
           E S+   K+    +T  +     T S   S+P  R+ S+ + N  +   P  +PSR   P
Sbjct: 92  ENSMVKLKEPKAKSTALKQREPLTRSVKVSKPTPRTVSSKQANTAAEVKPSIKPSRQATP 151

Query: 323 LIISRPSKPSEVQNFPLDEAARPLSERPASATRGRPVAASSAKSSSL-RAMSNGK--TRQ 382
              SR + PS       + A +  SE   ++ +    A++  +SSS+ +++S+ K  T +
Sbjct: 152 --TSRATLPSTRLT---NSAQKSNSESITTSIKSNTRASAFPRSSSVGKSVSSAKCTTPK 211

Query: 383 KPSSPSKQLASNRSSAYNSGKVFPFKQKIRSSEENEVSPVIMGTKMVERVVNMRKLAPPK 442
            P + ++ ++++RS  Y+SG     +   RS   N+V+PV+MGT+MVERVVNMRKL PPK
Sbjct: 212 IPETRARPVSTSRSRVYSSGD----RSTGRSKISNDVNPVLMGTQMVERVVNMRKLPPPK 271

Query: 443 QGSYRSSSGDPSSKSSIDATGFGRTLSNKSLDMALRHMDITLSISGKVRPVVTKTQASSI 502
                +              GFGRTLS  SLDMALRHM+I  S+S  +R V      +S+
Sbjct: 272 HDDNTT-------------LGFGRTLSRSSLDMALRHMNIRHSVSKNLR-VTVSASTNSM 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027390.12.0e-286100.00hypothetical protein SDJN02_11402 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6595382.11.2e-22786.26hypothetical protein SDJN03_11935, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022966648.11.5e-22083.91TRIO and F-actin-binding protein-like [Cucurbita maxima][more]
XP_022931827.11.9e-22083.73TRIO and F-actin-binding protein-like [Cucurbita moschata][more]
XP_023517474.14.3e-22083.94TRIO and F-actin-binding protein-like [Cucurbita pepo subsp. pepo] >XP_023517475... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1HSQ17.2e-22183.91TRIO and F-actin-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC11146627... [more]
A0A6J1EUT59.4e-22183.73TRIO and F-actin-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111438... [more]
A0A0A0KXX81.6e-18070.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G308580 PE=4 SV=1[more]
A0A6J1DET03.5e-17570.57probable serine/threonine-protein kinase nek3 OS=Momordica charantia OX=3673 GN=... [more]
A0A5D3BCG13.9e-17468.76Cell wall protein DAN4-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT2G40070.11.0e-4639.84BEST Arabidopsis thaliana protein match is: proline-rich family protein (TAIR:AT... [more]
AT2G40070.21.0e-4639.84FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G09000.11.6e-4241.10proline-rich family protein [more]
AT5G01280.17.5e-1330.00BEST Arabidopsis thaliana protein match is: proline-rich family protein (TAIR:AT... [more]
AT2G38160.13.7e-1233.88unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..317
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 492..553
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 492..537
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 433..460
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 357..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 438..460
NoneNo IPR availablePANTHERPTHR31949GASTRIC MUCIN-LIKE PROTEINcoord: 130..549
NoneNo IPR availablePANTHERPTHR31949:SF15GPI-ANCHORED PROTEIN PB15E9.01C-RELATEDcoord: 130..549

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13270-RACarg13270-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043622 cortical microtubule organization
cellular_component GO:0055028 cortical microtubule