Carg13251 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13251
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	glutathione S-transferase U8-like
Location	Carg_Chr07: 5841481 .. 5841693 (-)
RNA-Seq Expression	Carg13251
Synteny	Carg13251

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGTTGTGGACATTGTAGGGTTCGTGTTTGCTGGGTGGATTCCTGCCATTGAAGAAGCGGTGGGATTTAAGCTATTGAACCACAAGTTTCCAAACCTAAGAAAATGGATCGAAGAGTATGCGAACCATACTGTGGCTAAGCAGGTTCTACCCGAAAAGGATGCGCTTGTTGTCTTTATAAAAAATGTTTCGCTTAAGCGAAAATATTAA

mRNA sequence

ATGGGGGTTGTGGACATTGTAGGGTTCGTGTTTGCTGGGTGGATTCCTGCCATTGAAGAAGCGGTGGGATTTAAGCTATTGAACCACAAGTTTCCAAACCTAAGAAAATGGATCGAAGAGTATGCGAACCATACTGTGGCTAAGCAGGTTCTACCCGAAAAGGATGCGCTTGTTGTCTTTATAAAAAATGTTTCGCTTAAGCGAAAATATTAA

Coding sequence (CDS)

ATGGGGGTTGTGGACATTGTAGGGTTCGTGTTTGCTGGGTGGATTCCTGCCATTGAAGAAGCGGTGGGATTTAAGCTATTGAACCACAAGTTTCCAAACCTAAGAAAATGGATCGAAGAGTATGCGAACCATACTGTGGCTAAGCAGGTTCTACCCGAAAAGGATGCGCTTGTTGTCTTTATAAAAAATGTTTCGCTTAAGCGAAAATATTAA

Protein sequence

MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVFIKNVSLKRKY

Homology

[Close]

BLAST of Carg13251 vs. NCBI nr
Match: KAG6595363.1 (putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 144.1 bits (362), Expect = 4.7e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
           MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF
Sbjct: 151 MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 210

Query: 61  IKNVSLKRKY 71
           IKNVSLKRKY
Sbjct: 211 IKNVSLKRKY 220

[Close]

BLAST of Carg13251 vs. NCBI nr
Match: KAG7027371.1 (putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 144.1 bits (362), Expect = 4.7e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
          MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF
Sbjct: 1  MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60

Query: 61 IKNVSLKRKY 71
          IKNVSLKRKY
Sbjct: 61 IKNVSLKRKY 70

[Close]

BLAST of Carg13251 vs. NCBI nr
Match: XP_022966639.1 (probable glutathione S-transferase [Cucurbita maxima])

HSP 1 Score: 143.3 bits (360), Expect = 8.0e-31
Identity = 68/70 (97.14%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
           MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQ+LPEKDALV+F
Sbjct: 151 MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQILPEKDALVIF 210

Query: 61  IKNVSLKRKY 71
           IKNVSLKRKY
Sbjct: 211 IKNVSLKRKY 220

[Close]

BLAST of Carg13251 vs. NCBI nr
Match: XP_022931954.1 (probable glutathione S-transferase [Cucurbita moschata])

HSP 1 Score: 142.5 bits (358), Expect = 1.4e-30
Identity = 68/70 (97.14%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
           MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWI+EYANHTVAKQVLPEKDALV+F
Sbjct: 151 MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIDEYANHTVAKQVLPEKDALVIF 210

Query: 61  IKNVSLKRKY 71
           IKNVSLKRKY
Sbjct: 211 IKNVSLKRKY 220

[Close]

BLAST of Carg13251 vs. NCBI nr
Match: KAG6595362.1 (putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107.1 bits (266), Expect = 6.4e-20
Identity = 50/70 (71.43%), Postives = 61/70 (87.14%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G+VDIVG + AGWIPAIEEA+GF+LL  HKFPNL+KW EE+ NH VAKQV+P+KDALV 
Sbjct: 151 IGLVDIVGLILAGWIPAIEEALGFELLIAHKFPNLKKWTEEFVNHHVAKQVMPQKDALVA 210

Query: 61  FIKNVSLKRK 70
           F+KNV+L RK
Sbjct: 211 FLKNVALNRK 220

[Close]

BLAST of Carg13251 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.9e-07
Identity = 26/62 (41.94%), Postives = 35/62 (56.45%), Query Frame = 0


Query: 2   GVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 61
           G+VDI     A WIP  +E  G +L  + KFP L KW +E+ NH    +VLP +D L  +
Sbjct: 154 GLVDIAAVFIAFWIPIFQEIAGLQLFTSEKFPILYKWSQEFLNHPFVHEVLPPRDPLFAY 213

Query: 62  IK 63
            K
Sbjct: 214 FK 215

[Close]

BLAST of Carg13251 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 7.9e-05
Identity = 25/63 (39.68%), Postives = 35/63 (55.56%), Query Frame = 0


Query: 2   GVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 61
           G  DI   + A W+   EEA G  L+ + KFPN  KW  EY N +  K+ LP +D L+ F
Sbjct: 148 GFADIAANLVAFWLGVFEEASGVVLVTSEKFPNFCKWRGEYINCSQIKESLPPRDELLAF 207

Query: 62  IKN 64
            ++
Sbjct: 208 YRS 210

[Close]

BLAST of Carg13251 vs. ExPASy Swiss-Prot
Match: Q03664 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 1.8e-04
Identity = 22/62 (35.48%), Postives = 33/62 (53.23%), Query Frame = 0


Query: 2   GVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 61
           G  DI   +   W+   EE  G  L+ + KFPN  +W +EY N +  K+ LP +D L+ F
Sbjct: 148 GFADIAANLVGFWLGVFEEGYGVVLVTSEKFPNFSRWRDEYINCSQVKESLPSRDELLAF 207

Query: 62  IK 63
            +
Sbjct: 208 FR 209

[Close]

BLAST of Carg13251 vs. ExPASy Swiss-Prot
Match: Q9ZW24 (Glutathione S-transferase U7 OS=Arabidopsis thaliana OX=3702 GN=GSTU7 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.1e-04
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLN-HKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G VDIV  + A W+   EE VG K++   KFP + +W++    + V K+ +P +D  + 
Sbjct: 154 LGFVDIVATLVAFWLMRTEEIVGVKVVPVEKFPEIHRWVKNLLGNDVIKKCIPPEDEHLK 213

Query: 61  FIK 63
           +I+
Sbjct: 214 YIR 216

[Close]

BLAST of Carg13251 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 6.7e-04
Identity = 22/62 (35.48%), Postives = 32/62 (51.61%), Query Frame = 0


Query: 2   GVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 61
           G  DI   +   W+   EE  G  L+ + KFPN  KW +EY N +   + LP +D L+ F
Sbjct: 148 GFADIAANLVGFWLGVFEEGYGDVLVKSEKFPNFSKWRDEYINCSQVNESLPPRDELLAF 207

Query: 62  IK 63
            +
Sbjct: 208 FR 209

[Close]

BLAST of Carg13251 vs. ExPASy TrEMBL
Match: A0A6J1HUD5 (probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466263 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.9e-31
Identity = 68/70 (97.14%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
           MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQ+LPEKDALV+F
Sbjct: 151 MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQILPEKDALVIF 210

Query: 61  IKNVSLKRKY 71
           IKNVSLKRKY
Sbjct: 211 IKNVSLKRKY 220

[Close]

BLAST of Carg13251 vs. ExPASy TrEMBL
Match: A0A6J1EVP2 (probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438220 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 6.6e-31
Identity = 68/70 (97.14%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIEEYANHTVAKQVLPEKDALVVF 60
           MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWI+EYANHTVAKQVLPEKDALV+F
Sbjct: 151 MGVVDIVGFVFAGWIPAIEEAVGFKLLNHKFPNLRKWIDEYANHTVAKQVLPEKDALVIF 210

Query: 61  IKNVSLKRKY 71
           IKNVSLKRKY
Sbjct: 211 IKNVSLKRKY 220

[Close]

BLAST of Carg13251 vs. ExPASy TrEMBL
Match: A0A6J1HSP0 (probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466262 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.1e-20
Identity = 50/70 (71.43%), Postives = 60/70 (85.71%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLN-HKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G+VDIVG + AGWIPAIEEA+GF+LLN HK PNL+KW EE+ NH VAKQV+P+KDALV 
Sbjct: 151 IGLVDIVGLILAGWIPAIEEALGFELLNTHKLPNLKKWTEEFVNHRVAKQVMPQKDALVA 210

Query: 61  FIKNVSLKRK 70
           F+KNV L RK
Sbjct: 211 FLKNVVLNRK 220

[Close]

BLAST of Carg13251 vs. ExPASy TrEMBL
Match: A0A6J1F079 (probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438219 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-19
Identity = 48/70 (68.57%), Postives = 60/70 (85.71%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLL-NHKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G+VDIVG + AGWIPAIEEA+GF+LL  H+FPNL+KW EE+ NH VAKQV+P+KD LV 
Sbjct: 151 IGLVDIVGLILAGWIPAIEEALGFELLITHRFPNLKKWTEEFVNHHVAKQVMPQKDGLVA 210

Query: 61  FIKNVSLKRK 70
           F+KNV+L RK
Sbjct: 211 FLKNVALNRK 220

[Close]

BLAST of Carg13251 vs. ExPASy TrEMBL
Match: A0A5D3DJ09 (Glutathione S-transferase U8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001410 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.7e-16
Identity = 45/70 (64.29%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLN-HKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G VDI G V A WIPA EEAVGF+LL+ HKFPNL KW +++ NH+VAK+VLPEKD LV 
Sbjct: 154 IGFVDIAGLVLARWIPATEEAVGFELLSAHKFPNLTKWSQDFVNHSVAKEVLPEKDVLVA 213

Query: 61  FIKNVSLKRK 70
           F+KNV    K
Sbjct: 214 FLKNVVFSTK 223

[Close]

BLAST of Carg13251 vs. TAIR 10
Match: AT2G29420.1 (glutathione S-transferase tau 7 )

HSP 1 Score: 44.7 bits (104), Expect = 3.6e-05
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 1   MGVVDIVGFVFAGWIPAIEEAVGFKLLN-HKFPNLRKWIEEYANHTVAKQVLPEKDALVV 60
           +G VDIV  + A W+   EE VG K++   KFP + +W++    + V K+ +P +D  + 
Sbjct: 154 LGFVDIVATLVAFWLMRTEEIVGVKVVPVEKFPEIHRWVKNLLGNDVIKKCIPPEDEHLK 213

Query: 61  FIK 63
           +I+
Sbjct: 214 YIR 216

The following BLAST results are available for this feature:

BLAST of Carg13251 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6595363.1	4.7e-31	100.00	putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7027371.1	4.7e-31	100.00	putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022966639.1	8.0e-31	97.14	probable glutathione S-transferase [Cucurbita maxima]	[more]
XP_022931954.1	1.4e-30	97.14	probable glutathione S-transferase [Cucurbita moschata]	[more]
KAG6595362.1	6.4e-20	71.43	putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Carg13251 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32110	4.9e-07	41.94	Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1	[more]
Q03662	7.9e-05	39.68	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03664	1.8e-04	35.48	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9ZW24	5.1e-04	33.33	Glutathione S-transferase U7 OS=Arabidopsis thaliana OX=3702 GN=GSTU7 PE=2 SV=1	[more]
Q03663	6.7e-04	35.48	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Carg13251 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HUD5	3.9e-31	97.14	probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466263 P...	[more]
A0A6J1EVP2	6.6e-31	97.14	probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438220...	[more]
A0A6J1HSP0	3.1e-20	71.43	probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111466262 P...	[more]
A0A6J1F079	1.2e-19	68.57	probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111438219...	[more]
A0A5D3DJ09	2.7e-16	64.29	Glutathione S-transferase U8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Carg13251 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29420.1	3.6e-05	33.33	glutathione S-transferase tau 7	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1050.10		coord: 1..69 e-value: 3.8E-17 score: 64.4
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 2..64
None	No IPR available	PANTHER	PTHR11260:SF651	GLUTATHIONE S-TRANSFERASE, AMINO-TERMINAL DOMAIN PROTEIN	coord: 2..64
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13251-RA	Carg13251-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004364	glutathione transferase activity