Carg13236 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13236
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein MIZU-KUSSEI 1
Location	Carg_Chr07: 5687016 .. 5687753 (+)
RNA-Seq Expression	Carg13236
Synteny	Carg13236

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAGATCGACGCCCTACGTCGGTACCTCCTCCCATGTTTCTTTCCCCCCACCGCAACAGCCACAGCCTCCTCCGCCGCCCCCAAGAAACGCCTAAGCACCTCGCTTCGCGACGACGTTGAAACCACCACCAACGGCTATCCAACCGACGACCAAGATTCCCCATCCACCACTCCCGACAGCGTCACACCAAAATTCTGTACCGCCGCCACTGCCATCGTGGCCCCACCACGGCCCTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACAGACGTGGCCACATGTGGTTCTGCGTCCAACACGACCGGCTACGAACTAAACCGTTTCTCCTCCTCGAGTTACCGATTCTGACTCACCAACTCGTCAACGAAATGCGGTTCGGACTCGTCCGAATCGCCCTTGAATACAATGGGGTCGAACTCGAGTTTTGCCCTCTCCGCTCAATCCCCATTTGGGCAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCAGCCAGGAAAAAAGGCGGTGACCCGATCCGGTCCATGCTAAAGACAATGCAATCGACAACAGTCGGAGCCGGTGTAATCCCATCCGGATTTGGGTCGGACTCGGAGGAGATCATGTACATGAGAGCTAATTATGAGCACGTGGTGGGCAGCGCTGACTCGGAGTCATTTCATCTGATCAACCCGGACGAGTACCCAGGTCAAGAACTTAGTATCTTCTTGCTCAGATCTCGTAATGGGTAA

mRNA sequence

ATGGCCAAGATCGACGCCCTACGTCGGTACCTCCTCCCATGTTTCTTTCCCCCCACCGCAACAGCCACAGCCTCCTCCGCCGCCCCCAAGAAACGCCTAAGCACCTCGCTTCGCGACGACGTTGAAACCACCACCAACGGCTATCCAACCGACGACCAAGATTCCCCATCCACCACTCCCGACAGCGTCACACCAAAATTCTGTACCGCCGCCACTGCCATCGTGGCCCCACCACGGCCCTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACAGACGTGGCCACATGTGGTTCTGCGTCCAACACGACCGGCTACGAACTAAACCGTTTCTCCTCCTCGAGTTACCGATTCTGACTCACCAACTCGTCAACGAAATGCGGTTCGGACTCGTCCGAATCGCCCTTGAATACAATGGGGTCGAACTCGAGTTTTGCCCTCTCCGCTCAATCCCCATTTGGGCAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCAGCCAGGAAAAAAGGCGGTGACCCGATCCGGTCCATGCTAAAGACAATGCAATCGACAACAGTCGGAGCCGGTGTAATCCCATCCGGATTTGGGTCGGACTCGGAGGAGATCATGTACATGAGAGCTAATTATGAGCACGTGGTGGGCAGCGCTGACTCGGAGTCATTTCATCTGATCAACCCGGACGAGTACCCAGGTCAAGAACTTAGTATCTTCTTGCTCAGATCTCGTAATGGGTAA

Coding sequence (CDS)

ATGGCCAAGATCGACGCCCTACGTCGGTACCTCCTCCCATGTTTCTTTCCCCCCACCGCAACAGCCACAGCCTCCTCCGCCGCCCCCAAGAAACGCCTAAGCACCTCGCTTCGCGACGACGTTGAAACCACCACCAACGGCTATCCAACCGACGACCAAGATTCCCCATCCACCACTCCCGACAGCGTCACACCAAAATTCTGTACCGCCGCCACTGCCATCGTGGCCCCACCACGGCCCTCGAAAACCATGGTCATCGGAACCATCTTCGGCCACAGACGTGGCCACATGTGGTTCTGCGTCCAACACGACCGGCTACGAACTAAACCGTTTCTCCTCCTCGAGTTACCGATTCTGACTCACCAACTCGTCAACGAAATGCGGTTCGGACTCGTCCGAATCGCCCTTGAATACAATGGGGTCGAACTCGAGTTTTGCCCTCTCCGCTCAATCCCCATTTGGGCAATGTCCTGCAACGGCCGGAAACTCGGGTTCGCAGCCAGGAAAAAAGGCGGTGACCCGATCCGGTCCATGCTAAAGACAATGCAATCGACAACAGTCGGAGCCGGTGTAATCCCATCCGGATTTGGGTCGGACTCGGAGGAGATCATGTACATGAGAGCTAATTATGAGCACGTGGTGGGCAGCGCTGACTCGGAGTCATTTCATCTGATCAACCCGGACGAGTACCCAGGTCAAGAACTTAGTATCTTCTTGCTCAGATCTCGTAATGGGTAA

Protein sequence

MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLLRSRNG

Homology

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BLAST of Carg13236 vs. NCBI nr
Match: KAG7027356.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 496.9 bits (1278), Expect = 1.0e-136
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

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BLAST of Carg13236 vs. NCBI nr
Match: XP_022931935.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata] >KAG6595348.1 Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493.8 bits (1270), Expect = 8.5e-136
Identity = 243/245 (99.18%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVEL FCPLRSIPIWAMSCNGRKLGFAARKKGGDP+RSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGGDPVRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

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BLAST of Carg13236 vs. NCBI nr
Match: XP_023517128.1 (protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.5 bits (1264), Expect = 4.2e-135
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVEL FCPLRSIPIWAMSCNGRKLGFAARKK GDP+RSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKSGDPVRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

[Close]

BLAST of Carg13236 vs. NCBI nr
Match: XP_022966571.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 490.0 bits (1260), Expect = 1.2e-134
Identity = 240/245 (97.96%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPT+TATAS+A PKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTSTATASAATPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVEL FCPLRSIPIWAMSCNGRKLGFAARKKGGDP+RSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGGDPVRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

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BLAST of Carg13236 vs. NCBI nr
Match: XP_004142185.2 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017911 [Cucumis sativus])

HSP 1 Score: 426.0 bits (1094), Expect = 2.2e-115
Identity = 217/249 (87.15%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPT-ATATASSAAPKKRLSTSLRDDVETTT---NGYPTDDQDSP 60
           MAKIDALRR+LLPCFFPPT ATA ASSA PKKRLSTSLRDD+ETTT   N  PT  QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60

Query: 61  STTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLEL 120
           +TTPDSVTPKF  +A +IVAPPRPSKTMVIGTIFGHRRGH+WFCVQHDRLR KPFLLLE 
Sbjct: 61  ATTPDSVTPKFAVSA-SIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120

Query: 121 PILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIR 180
           PILTHQLVNEMRFGLVRIALE N VEL FCPLRSIPIWAMSCNGRKLGFAA+KK G+P+R
Sbjct: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVR 180

Query: 181 SMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELS 240
           SMLKTMQSTTVGAGV+PSGFGS SEE+MYMRANYEHVVGSADSESFHLINPDE PGQELS
Sbjct: 181 SMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELS 240

Query: 241 IFLLRSRNG 246
           IFLLRSRNG
Sbjct: 241 IFLLRSRNG 248

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BLAST of Carg13236 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.6e-31
Identity = 97/248 (39.11%), Postives = 132/248 (53.23%), Query Frame = 0


Query: 10  YLLPC----FFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTPDSVTP 69
           +L+PC    + P +++++AS    K  L  SL           P     S  + P S + 
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSSSS 109

Query: 70  KFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILTHQLVN 129
                 + +          V GT++GH+RGH+ F VQ+++ R+ P LLL+L + T  LV 
Sbjct: 110 VSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATLVK 169

Query: 130 EMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGG--DPIRSMLKTMQ 189
           EM  GLVRIALE          L   P W M CNGRK G+A  + G   D    +L T+ 
Sbjct: 170 EMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVS 229

Query: 190 STTVGAGVIP--------SGFGSDSE--EIMYMRANYEHVVGSADSESFHLINPDEYPGQ 242
             TVGAGVIP        SG GS +E  E++YMR  +E VVGS DSE+F+++NPD+  G 
Sbjct: 230 RVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGP 289

[Close]

BLAST of Carg13236 vs. ExPASy TrEMBL
Match: A0A6J1F0T8 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 4.1e-136
Identity = 243/245 (99.18%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVEL FCPLRSIPIWAMSCNGRKLGFAARKKGGDP+RSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGGDPVRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

[Close]

BLAST of Carg13236 vs. ExPASy TrEMBL
Match: A0A6J1HPP6 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466214 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 5.9e-135
Identity = 240/245 (97.96%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60
           MAKIDALRRYLLPCFFPPT+TATAS+A PKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP
Sbjct: 1   MAKIDALRRYLLPCFFPPTSTATASAATPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTP 60

Query: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120
           DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT
Sbjct: 61  DSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILT 120

Query: 121 HQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLK 180
           HQLVNEMRFGLVRIALEYNGVEL FCPLRSIPIWAMSCNGRKLGFAARKKGGDP+RSMLK
Sbjct: 121 HQLVNEMRFGLVRIALEYNGVELGFCPLRSIPIWAMSCNGRKLGFAARKKGGDPVRSMLK 180

Query: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240
           TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL
Sbjct: 181 TMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLL 240

Query: 241 RSRNG 246
           RSRNG
Sbjct: 241 RSRNG 245

[Close]

BLAST of Carg13236 vs. ExPASy TrEMBL
Match: A0A0A0KXC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.1e-115
Identity = 217/249 (87.15%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPT-ATATASSAAPKKRLSTSLRDDVETTT---NGYPTDDQDSP 60
           MAKIDALRR+LLPCFFPPT ATA ASSA PKKRLSTSLRDD+ETTT   N  PT  QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSAVPKKRLSTSLRDDLETTTATLNEDPTHGQDSP 60

Query: 61  STTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLEL 120
           +TTPDSVTPKF  +A +IVAPPRPSKTMVIGTIFGHRRGH+WFCVQHDRLR KPFLLLE 
Sbjct: 61  ATTPDSVTPKFAVSA-SIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120

Query: 121 PILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIR 180
           PILTHQLVNEMRFGLVRIALE N VEL FCPLRSIPIWAMSCNGRKLGFAA+KK G+P+R
Sbjct: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGEPVR 180

Query: 181 SMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELS 240
           SMLKTMQSTTVGAGV+PSGFGS SEE+MYMRANYEHVVGSADSESFHLINPDE PGQELS
Sbjct: 181 SMLKTMQSTTVGAGVMPSGFGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELS 240

Query: 241 IFLLRSRNG 246
           IFLLRSRNG
Sbjct: 241 IFLLRSRNG 248

[Close]

BLAST of Carg13236 vs. ExPASy TrEMBL
Match: A0A6J1ISJ8 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.7e-113
Identity = 217/253 (85.77%), Postives = 227/253 (89.72%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPTA-TATASSAAPKKRLSTSLRDDVE----TTTNGYPTDDQDS 60
           MAKIDALRR+ LPCFFPPTA TATA+SAAPKKRLSTSLRDD+E    TT NG PT DQDS
Sbjct: 1   MAKIDALRRFFLPCFFPPTATTATAASAAPKKRLSTSLRDDLEISASTTANGGPTHDQDS 60

Query: 61  PSTTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLE 120
           P+TTPDSVTPKF TAA AIVAPPRPSKTMVIGTIFGHRRGH+WFCVQ DRLR KPFLLLE
Sbjct: 61  PATTPDSVTPKFVTAA-AIVAPPRPSKTMVIGTIFGHRRGHVWFCVQQDRLRNKPFLLLE 120

Query: 121 LPILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPI 180
            PILTHQLVNEMRFGLVRIALE N VEL  CPLRSIPIWAMSCNGRKLGFAA+KK GD I
Sbjct: 121 FPILTHQLVNEMRFGLVRIALECNRVELGLCPLRSIPIWAMSCNGRKLGFAAKKKAGDSI 180

Query: 181 RSMLKTMQSTTVGAGVIPSGF----GSDSEEIMYMRANYEHVVGSADSESFHLINPDEYP 240
           RSMLKTMQSTTVGAGV+PSGF    GS +EE+MYMRANYEHVVGSADSESFHLINPDE+P
Sbjct: 181 RSMLKTMQSTTVGAGVMPSGFGFGSGSKTEEVMYMRANYEHVVGSADSESFHLINPDEFP 240

Query: 241 GQELSIFLLRSRN 245
           GQELSIFLLRS N
Sbjct: 241 GQELSIFLLRSPN 252

[Close]

BLAST of Carg13236 vs. ExPASy TrEMBL
Match: A0A5D3DK02 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001000 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.3e-113
Identity = 213/249 (85.54%), Postives = 225/249 (90.36%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFPPT-ATATASSAAPKKRLSTSLRDDVETTT---NGYPTDDQDSP 60
           MAKIDALRR+LLPCFFPPT ATA ASS  PKKRLSTSLRDD+ETTT   +  PT  QDSP
Sbjct: 1   MAKIDALRRFLLPCFFPPTAATAAASSTVPKKRLSTSLRDDLETTTATVSEDPTHAQDSP 60

Query: 61  STTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLEL 120
           +TTPDSVTPKF  +A +IVAPPRPSKTMVIGTIFGHRRGH+WFCVQHDRLR KPFLLLE 
Sbjct: 61  ATTPDSVTPKFAISA-SIVAPPRPSKTMVIGTIFGHRRGHVWFCVQHDRLRNKPFLLLEF 120

Query: 121 PILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIR 180
           PILTHQLVNEMRFGLVRIALE N VEL FCPLRSIPIWAMSCNGRKLGFAA+KK G+ +R
Sbjct: 121 PILTHQLVNEMRFGLVRIALECNRVELGFCPLRSIPIWAMSCNGRKLGFAAKKKAGESVR 180

Query: 181 SMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELS 240
           SMLKTMQSTTVGAGV+PSG GS SEE+MYMRANYEHVVGSADSESFHLINPDE PGQELS
Sbjct: 181 SMLKTMQSTTVGAGVMPSGLGSGSEEVMYMRANYEHVVGSADSESFHLINPDECPGQELS 240

Query: 241 IFLLRSRNG 246
           IFLLRSRNG
Sbjct: 241 IFLLRSRNG 248

[Close]

BLAST of Carg13236 vs. TAIR 10
Match: AT2G37880.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 277.3 bits (708), Expect = 1.2e-74
Identity = 149/252 (59.13%), Postives = 176/252 (69.84%), Query Frame = 0


Query: 1   MAKIDALRRYLLPCFFP---PTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPS 60
           M KID+LRR+LLPC      PT  +T S+ A KKRLSTSLRDD++         D  S S
Sbjct: 1   MTKIDSLRRFLLPCISTPTNPTTVSTTSTGASKKRLSTSLRDDID-------VQDSASSS 60

Query: 61  TTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELP 120
            +    T     + +A+  P RPSKTMVIGTIFG R+GH+WFCVQHDRL  KP LLLEL 
Sbjct: 61  ASSSEATSAVDYSISAVTVPQRPSKTMVIGTIFGRRKGHVWFCVQHDRLSVKPILLLELS 120

Query: 121 ILTHQLVNEMRFGLVRIALEY-NGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIR 180
           I T QLV+EM  GLVR+ALE     EL+ C LRS+P+W M CNGRKLGFA R+   +  R
Sbjct: 121 IATSQLVHEMGSGLVRVALECPTRPELKSCLLRSVPVWTMFCNGRKLGFAVRRSANEETR 180

Query: 181 SMLKTMQSTTVGAGVIPSGFG------SDSEEIMYMRANYEHVVGSADSESFHLINPDEY 240
            MLK ++S TVGAGV+PSG G      SD++E+MYMRANYEHVVGS+DSESFHLINPD  
Sbjct: 181 MMLKRLESMTVGAGVLPSGSGLGGSDESDTDEVMYMRANYEHVVGSSDSESFHLINPDAN 240

Query: 241 PGQELSIFLLRS 243
             QELSIFLLR+
Sbjct: 241 SAQELSIFLLRT 245

[Close]

BLAST of Carg13236 vs. TAIR 10
Match: AT1G21050.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 142.9 bits (359), Expect = 3.4e-34
Identity = 89/226 (39.38%), Postives = 130/226 (57.52%), Query Frame = 0


Query: 21  TATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTPDSVTPKFCTAATAIVAPPRP 80
           T T+   + + R   +LR  +E       T  Q        SV+    +++++  +    
Sbjct: 23  TPTSVDCSREFRHRRTLRSLIECMIPYCCTYQQQPYQNDTLSVSSTSSSSSSSDHSSSSQ 82

Query: 81  SKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILTHQLVNEM-RFGLVRIALEYN 140
           S + V GT FGHRRG + FC+Q   + + P LLLEL + T  L  EM   G++RIALE +
Sbjct: 83  SSSTVSGTFFGHRRGRVSFCLQDAAVGSSPLLLLELAVPTAALAKEMDEEGVLRIALECD 142

Query: 141 ---GVELEFCPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLKTMQSTTVGAGVIPSGF 200
                      +  +P+W+M CNGRK+GFA R+K  +     L+ MQS +VGAGV+PS  
Sbjct: 143 RRRSSNSRSSSIFDVPVWSMFCNGRKMGFAVRRKVTENDAVFLRMMQSVSVGAGVVPS-- 202

Query: 201 GSDSEEIMYMRANYEHVVGSADSESFHLINPDEYPGQELSIFLLRS 243
             + ++++Y+RA +E V GS+DSESFH++NP    GQELSIFLLRS
Sbjct: 203 -EEEDQMLYLRARFERVTGSSDSESFHMMNPGGSYGQELSIFLLRS 245

[Close]

BLAST of Carg13236 vs. TAIR 10
Match: AT1G76610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 142.5 bits (358), Expect = 4.5e-34
Identity = 83/191 (43.46%), Postives = 114/191 (59.69%), Query Frame = 0


Query: 53  QDSPSTTPDSVTPKFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHD-RLRTKPF 112
           Q  P+   D+V+    T+++        +  +V GT +GHRRGH+ FC+Q D R  + P 
Sbjct: 50  QPPPTDQNDTVSVSSSTSSS------DQTGVVVTGTFYGHRRGHVSFCLQDDTRPSSPPL 109

Query: 113 LLLELPILTHQLVNEMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKG 172
           LLLEL + T  L  EM  G +RIAL           + ++P+W+M CNGRK GFA R++ 
Sbjct: 110 LLLELAVPTAALAREMEEGFLRIALRSKSNRRS--SIFNVPVWSMYCNGRKFGFAVRRET 169

Query: 173 GDPIRSMLKTMQSTTVGAGVIPSGFGSDSEEIMYMRANYEHVVGSADSESFHLINPDEYP 232
            +     L+ MQS +VGAGVIP+G      E +Y+RA +E V GS+DSESFH++N     
Sbjct: 170 TENDVGFLRLMQSVSVGAGVIPNG------ETLYLRAKFERVTGSSDSESFHMVNQGGGY 226

Query: 233 GQELSIFLLRS 243
           GQELSIFL RS
Sbjct: 230 GQELSIFLSRS 226

[Close]

BLAST of Carg13236 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-32
Identity = 97/248 (39.11%), Postives = 132/248 (53.23%), Query Frame = 0


Query: 10  YLLPC----FFPPTATATASSAAPKKRLSTSLRDDVETTTNGYPTDDQDSPSTTPDSVTP 69
           +L+PC    + P +++++AS    K  L  SL           P     S  + P S + 
Sbjct: 50  FLVPCRRCSYVPLSSSSSASHNIGKFHLKFSLLLRSFINIINIPACKMLSLPSPPSSSSS 109

Query: 70  KFCTAATAIVAPPRPSKTMVIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILTHQLVN 129
                 + +          V GT++GH+RGH+ F VQ+++ R+ P LLL+L + T  LV 
Sbjct: 110 VSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSVQYNQ-RSDPVLLLDLAMSTATLVK 169

Query: 130 EMRFGLVRIALEYNGVELEFCPLRSIPIWAMSCNGRKLGFAARKKGG--DPIRSMLKTMQ 189
           EM  GLVRIALE          L   P W M CNGRK G+A  + G   D    +L T+ 
Sbjct: 170 EMSSGLVRIALECEKRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVS 229

Query: 190 STTVGAGVIP--------SGFGSDSE--EIMYMRANYEHVVGSADSESFHLINPDEYPGQ 242
             TVGAGVIP        SG GS +E  E++YMR  +E VVGS DSE+F+++NPD+  G 
Sbjct: 230 RVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGP 289

[Close]

BLAST of Carg13236 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-31
Identity = 77/170 (45.29%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 85  VIGTIFGHRRGHMWFCVQHDRLRTKPFLLLELPILTHQLVNEMRFGLVRIALEYNGVELE 144
           V+GT+FG+RRGH++F VQ D  R  P +L++LP  T  LV EM  GLVRIALE    + +
Sbjct: 98  VVGTLFGNRRGHVYFAVQDDPTRL-PAVLIQLPTPTSVLVREMASGLVRIALETAAYKTD 157

Query: 145 F-CPLRSIPIWAMSCNGRKLGFAARKKGGDPIRSMLKTMQSTTVGAGVIPS--------- 204
               L     W   CNG+K G+AARK+ G+    +LK +   T+GAGV+P+         
Sbjct: 158 SKKKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEG 217

Query: 205 --GFGSDSEEIMYMRANYEHVVGSADSESFHLINPD-EYPGQELSIFLLR 242
               GS+  E+MYMRA +E VVGS DSE+F+++NPD    G ELS++ LR
Sbjct: 218 NGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLR 266

The following BLAST results are available for this feature:

BLAST of Carg13236 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027356.1	1.0e-136	100.00	Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022931935.1	8.5e-136	99.18	protein MIZU-KUSSEI 1 [Cucurbita moschata] >KAG6595348.1 Protein MIZU-KUSSEI 1, ...	[more]
XP_023517128.1	4.2e-135	98.78	protein MIZU-KUSSEI 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022966571.1	1.2e-134	97.96	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
XP_004142185.2	2.2e-115	87.15	protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN54300.1 hypothetical protein Csa_017...	[more]

BLAST of Carg13236 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	2.6e-31	39.11	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of Carg13236 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0T8	4.1e-136	99.18	protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111438210 PE=4 SV=1	[more]
A0A6J1HPP6	5.9e-135	97.96	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466214 PE=4 SV=1	[more]
A0A0A0KXC2	1.1e-115	87.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297500 PE=4 SV=1	[more]
A0A6J1ISJ8	3.7e-113	85.77	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478303 PE=4 SV=1	[more]
A0A5D3DK02	8.3e-113	85.54	Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50...	[more]

BLAST of Carg13236 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37880.1	1.2e-74	59.13	Protein of unknown function, DUF617	[more]
AT1G21050.1	3.4e-34	39.38	Protein of unknown function, DUF617	[more]
AT1G76610.1	4.5e-34	43.46	Protein of unknown function, DUF617	[more]
AT2G41660.1	1.9e-32	39.11	Protein of unknown function, DUF617	[more]
AT3G25640.1	1.2e-31	45.29	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 85..241 e-value: 8.1E-57 score: 191.8
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 85..242 e-value: 3.6E-57 score: 190.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..63
None	No IPR available	PANTHER	PTHR31276	FAMILY NOT NAMED	coord: 1..243
None	No IPR available	PANTHER	PTHR31276:SF6	PROTEIN MIZU-KUSSEI 1	coord: 1..243

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13236-RA	Carg13236-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism