Carg13220 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13220
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)-bd_dom domain-containing protein
LocationCarg_Chr07: 5587126 .. 5590239 (-)
RNA-Seq ExpressionCarg13220
SyntenyCarg13220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAGTCCATGGCGATGCTAACACGTGTCCCATTAGCCTACTCCATCCCAACCCCGAAGTCTACCCTTTCTCCACCACTGCCCTTTCATCAATGTCATAATCGTTGTTCCCTGCCCAATTCCAATTCAATCTCGTTTCTCTCACTTCAACTTGTTACCTCCTCCATTTCTCCGACCTCCCAGAAGGGTTTCAGAAGGCGCCGCCGCGTCGGTGCTCTTTCGCTGGTTGCAATGGCGGATTCCGGTCCTAGAACTGTCCTCGTCACCGGAGCTGGTGGCAGAACCGGTAACTCTGCTCTTTTCTTTGCTTGTTTATTGTTGTTTTGCTTATGTTCGACTGTTGAGGAAATTGTAGGGCAGTTTAAGGCAGAATTAGTAGTGGGGTTTCGATTTTTTCTTCCGGGTATGGAATTTTATCTTCGTGTCTCTGGAACTGATTGTTCGATGTCTTCCCTGTTGAGGGTTTAGGTTTTATGTTGATTTGGTTTTTGATTTGGAAAATTGGAGCCACTGACTTAACTTTGTTGCACATGGGACGTTGAATATTAGTAGGTAGTGTTGAGGATGGTTGGTAGAGGAGTCCCACATCGACTAGCTAATTAAGGAGTTGATCATGGGTTTATAAGTAAGAAATACACCTTCACTGGTACGAGGCATTTTGAGGAAACCAAAAGCAACATTATGAGAATTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGGTTTCTGATGAAGTATCAAACTCATGTCCTTAACTTAGTCATGTCAATAGAATTCTCAAGTGTCAAACCAAGAAGTTGTGAGTCCCGAAGGTGTAGTCAAAAGTGACTAAAGTGTTGAACAAATAGTGTATTTTGTTTGAGGGCTCTAGAGGAGTCGAGCCTCAATTAGAGGGAGACTGTTCGAGGAATCCATAGGCCTTAGGGGAGGCTCTATGGTGTACTTTGTTAGCTGTCAACTCGAGTTCTCTATCTGATTGGTTCGCTTGTACACAAATCCTGCACCTTCGAAATTGGTTTATGGATTTGAGAACATGTTTGAAGTGATTTTCTTAGTGCTTAAAAAGGGTCATTAAGCATTTTGTATAAGCGCTTAGATTCCTAATAAGGTTTTGTTGGTCCTGTTTGGCAGGTCAACTAGTTTACAAGAAATTGAAGGAGAGGTCAGATGAGTATGTTGCAAGAGGTTTGGTTAGAACTGAAGAGAGCAAGCAGAGTATCGGTGGAGCAGACGATCTTTTCATTGGTGATATTAGGAATGCAGATACCCTTGGTCCTGCAATTCAAGGCATCGACGCGCTCATTATTCTTACGAGCGCCGTGCCAAAGATGAAGCCAGGGTTTGATCCAACCAAAGGTGGTAGGCCTGAGTTTTACTTCGAAGATGGAGCATATCCTGAACAGGTACCTCAACAGTCTCTTCTCTTCTTTTCTTTTCTTTTTGCACACCTTATTATGTATTTATGTGAGATCACACATTGGCTGGAAAGGAGAATGAAACATTTCTATAAGGGTGTGAAAATCTCTTTCTAGTAGACGCGTTTTAAAACCTTGAAGAGCCTAAAGAGGACAATATTTGCTAACGGTAGGCTTGAGCCATTATAAATGGTATAGAGTTAAACACTTGGCAGTGTGCCGACGAGAACGCTGGCCCCAAAGGGGGTGGATTGTGAGATCCCACATTGGTTGGAGAGGAGAACGAAACATTCCTTATAAAGGTGTGGAAACTTTCCCTAGTAGACGCATTTTAAAACCTTAAGGACCCCAATGAGGAAAATATTTGCTAGCGGTGAGCTTGAGCTTGGACTGTTACAATTTCGCTCTCTGATGGATGTTCGTGTGTAGGTTGATTGGATTGGCCAAAAGATTCAGATAGATACTGGTGAGTGATCGGATTGCTCTTTTTTTTTTCCGTATCGTTTTCATGAGTTTCAAATGCTGAAATCATATCATGATTCGAAACAGCCAAGGCTGCAGGAGTTAAGCAAATCGTTTTGGTCGGGTCGATGGGAGGAACAAATATCAACCATCCCTTGAACAGTTTGGGTAATGGGAACATATTGGTTAGTATTATCTTTCCAATTACTTGATCTTCTACTTCTGGAAATCGTGAGAAACAGTATCTAACTCTTTTCATTATATAAAAGGTTTGGAAAAGGAAGGCCGAGCAGTATTTGGCTGACTCTGGTGTTCCTTACACCATCATAAGGTACGTCGTTGATCGAGTTGATCGAGTTTCATATTCATTGTCACCACAGAGAGTTCTGTACCTTTGCTCAAAACATAACCACTTGAAATTCTCAGAAAAAGCTGTTTTTGTGTTCCCATCTGGTTGGAAATATTGTTACTATTCAACTTCACTCAGCTTGCTCAAACCTTTCTAAATGCAAACGTCGAACGACTGTGTTCCTGTTCGATTTAAGCGTTCTTTCTTTATCAATTTACCGAATTTGAATACCGTTTGTCAGGGCTGGAGGTCTGCAGGATAAGGACGGTGGGATACGAGAACTGCTAGTAGGGAAGGATGATGAACTTCTTCAGACGGAGACGAGAACCATTGCCAGGGCTGATGTGGCCGAAGTCTGCCTTCAGGTATTAAATACTATGAAATTATTCCAGGACGACTATTAGAAAAATACTATGATTGGTTATTGCTCACCGTCATCACCCATCATCGCTGAGCATATCAATGGACTACTGTAACTTGGCCACTGTATGGAGGGTTCGTCGATGGTGGGCAGTGGTCTCTGGTCGTGGAAGGTGATAGTGTGAGAGTATTTTAGTCATTTTGAAGAGATCTGAAGTTAAACATCTAAAAAAAAATCTATGGAATAGTCTATCATCTTTCCTATTTAACATTTAATCCAAGCTCGAAACTCTGGAACAGGCACTGCAATTTGAGGAGGCCAAATTCAAGGCGCTCGATCTAGCCTCGAAACCTGAGGGGGTAGGCACGCCGACGACCGATTTCAAGGCCTTGTTCTCTCAAGTGACGACGAGTTTCTGAGTGGGTCATGTTTGTAAGGTCATTCTTTTCTAATGTAGTATTGTTGCCCAGAATTGTTTGGCTGCTAATAAACTCTGAACTGTGAGCTTTGAAG

mRNA sequence

ATGAAGCAGTCCATGGCGATGCTAACACGTGTCCCATTAGCCTACTCCATCCCAACCCCGAAGTCTACCCTTTCTCCACCACTGCCCTTTCATCAATGTCATAATCGTTGTTCCCTGCCCAATTCCAATTCAATCTCGTTTCTCTCACTTCAACTTGTTACCTCCTCCATTTCTCCGACCTCCCAGAAGGGTTTCAGAAGGCGCCGCCGCGTCGGTGCTCTTTCGCTGGTTGCAATGGCGGATTCCGGTCCTAGAACTGTCCTCGTCACCGGAGCTGGTGGCAGAACCGGTCAACTAGTTTACAAGAAATTGAAGGAGAGGTCAGATGAGTATGTTGCAAGAGGTTTGGTTAGAACTGAAGAGAGCAAGCAGAGTATCGGTGGAGCAGACGATCTTTTCATTGGTGATATTAGGAATGCAGATACCCTTGGTCCTGCAATTCAAGGCATCGACGCGCTCATTATTCTTACGAGCGCCGCTGCAGGAGTTAAGCAAATCGTTTTGGTCGGGTCGATGGGAGGAACAAATATCAACCATCCCTTGAACAGTTTGGGTAATGGGAACATATTGGTTTGGAAAAGGAAGGCCGAGCAGTATTTGGCTGACTCTGGTGTTCCTTACACCATCATAAGGGCTGGAGGTCTGCAGGATAAGGACGGTGGGATACGAGAACTGCTAGTAGGGAAGGATGATGAACTTCTTCAGACGGAGACGAGAACCATTGCCAGGGCTGATGTGGCCGAAGTCTGCCTTCAGGCACTGCAATTTGAGGAGGCCAAATTCAAGGCGCTCGATCTAGCCTCGAAACCTGAGGGGGTAGGCACGCCGACGACCGATTTCAAGGCCTTGTTCTCTCAAGTGACGACGAGTTTCTGAGTGGGTCATGTTTGTAAGGTCATTCTTTTCTAATGTAGTATTGTTGCCCAGAATTGTTTGGCTGCTAATAAACTCTGAACTGTGAGCTTTGAAG

Coding sequence (CDS)

ATGAAGCAGTCCATGGCGATGCTAACACGTGTCCCATTAGCCTACTCCATCCCAACCCCGAAGTCTACCCTTTCTCCACCACTGCCCTTTCATCAATGTCATAATCGTTGTTCCCTGCCCAATTCCAATTCAATCTCGTTTCTCTCACTTCAACTTGTTACCTCCTCCATTTCTCCGACCTCCCAGAAGGGTTTCAGAAGGCGCCGCCGCGTCGGTGCTCTTTCGCTGGTTGCAATGGCGGATTCCGGTCCTAGAACTGTCCTCGTCACCGGAGCTGGTGGCAGAACCGGTCAACTAGTTTACAAGAAATTGAAGGAGAGGTCAGATGAGTATGTTGCAAGAGGTTTGGTTAGAACTGAAGAGAGCAAGCAGAGTATCGGTGGAGCAGACGATCTTTTCATTGGTGATATTAGGAATGCAGATACCCTTGGTCCTGCAATTCAAGGCATCGACGCGCTCATTATTCTTACGAGCGCCGCTGCAGGAGTTAAGCAAATCGTTTTGGTCGGGTCGATGGGAGGAACAAATATCAACCATCCCTTGAACAGTTTGGGTAATGGGAACATATTGGTTTGGAAAAGGAAGGCCGAGCAGTATTTGGCTGACTCTGGTGTTCCTTACACCATCATAAGGGCTGGAGGTCTGCAGGATAAGGACGGTGGGATACGAGAACTGCTAGTAGGGAAGGATGATGAACTTCTTCAGACGGAGACGAGAACCATTGCCAGGGCTGATGTGGCCGAAGTCTGCCTTCAGGCACTGCAATTTGAGGAGGCCAAATTCAAGGCGCTCGATCTAGCCTCGAAACCTGAGGGGGTAGGCACGCCGACGACCGATTTCAAGGCCTTGTTCTCTCAAGTGACGACGAGTTTCTGA

Protein sequence

MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF
Homology
BLAST of Carg13220 vs. NCBI nr
Match: KAG7027340.1 (putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT
Sbjct: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE
Sbjct: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAAAGVKQIVLVGSMGGTNINHP 180
           ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAAAGVKQIVLVGSMGGTNINHP
Sbjct: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAAAGVKQIVLVGSMGGTNINHP 180

Query: 181 LNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRT 240
           LNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRT
Sbjct: 181 LNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRT 240

Query: 241 IARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF 292
           IARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF
Sbjct: 241 IARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF 291

BLAST of Carg13220 vs. NCBI nr
Match: KAG6595330.1 (putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 538.1 bits (1385), Expect = 4.7e-149
Identity = 290/333 (87.09%), Postives = 290/333 (87.09%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT
Sbjct: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE
Sbjct: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGADDLFIGDIRNADTLGPAIQGID LIILTSA                     
Sbjct: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDVLIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQKNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. NCBI nr
Match: XP_022931843.1 (uncharacterized protein At5g02240 [Cucurbita moschata])

HSP 1 Score: 532.3 bits (1370), Expect = 2.6e-147
Identity = 287/333 (86.19%), Postives = 288/333 (86.49%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSIS LS Q+VTSSISPT
Sbjct: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISSLSFQIVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE
Sbjct: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA                     
Sbjct: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQKNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. NCBI nr
Match: XP_023518926.1 (uncharacterized protein At5g02240-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 529.3 bits (1362), Expect = 2.2e-146
Identity = 285/333 (85.59%), Postives = 288/333 (86.49%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHN CSLPNSNSIS LSLQ+VTSSISPT
Sbjct: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNCCSLPNSNSISSLSLQIVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSD+YVARGLVRTE
Sbjct: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDQYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGADDLF+GDIRNADTLGPAIQGIDALIILTSA                     
Sbjct: 121 ESKQSIGGADDLFVGDIRNADTLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQRNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. NCBI nr
Match: XP_022966108.1 (uncharacterized protein At5g02240-like [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.1e-141
Identity = 278/333 (83.48%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMA LTRVPLAYSIP+PKSTLS P PFHQCHN CSLPNSNSISFLSLQ+VTSSISPT
Sbjct: 1   MKQSMATLTRVPLAYSIPSPKSTLSTPPPFHQCHNCCSLPNSNSISFLSLQIVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQK FRRR RVGALSLVAMADSG RTVLVTGAGGRTGQLVYKKLKERSD+YVARGLVRTE
Sbjct: 61  SQKSFRRRCRVGALSLVAMADSGSRTVLVTGAGGRTGQLVYKKLKERSDQYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGA+DLFIGDIRNADTLGPAIQGIDALIILTSA                     
Sbjct: 121 ESKQSIGGAEDLFIGDIRNADTLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQKNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSG+PYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. ExPASy Swiss-Prot
Match: O80934 (Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g37660 PE=1 SV=2)

HSP 1 Score: 348.6 bits (893), Expect = 6.9e-95
Identity = 191/296 (64.53%), Postives = 220/296 (74.32%), Query Frame = 0

Query: 38  SLPNSNSISFLSLQLVTSSISPTSQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTG 97
           S+P S+S+ F S  LV+ S S      F  + R  ++++ A A + P TVLVTGAGGRTG
Sbjct: 32  SVPRSSSLQFRS--LVSDSTSICGPSKFTGKNRRVSVTVSAAATTEPLTVLVTGAGGRTG 91

Query: 98  QLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILT 157
           Q+VYKKLKERS+++VARGLVRT+ESK+ I G D++FIGDIR+  ++ PA++GIDAL+ILT
Sbjct: 92  QIVYKKLKERSEQFVARGLVRTKESKEKINGEDEVFIGDIRDTASIAPAVEGIDALVILT 151

Query: 158 SA------------------------------------------AAGVKQIVLVGSMGGT 217
           SA                                          AAGVKQIVLVGSMGGT
Sbjct: 152 SAVPQMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGT 211

Query: 218 NINHPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQ 277
           NINHPLNS+GN NILVWKRKAEQYLADSG+PYTIIRAGGLQDKDGGIRELLVGKDDELL+
Sbjct: 212 NINHPLNSIGNANILVWKRKAEQYLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDELLE 271

Query: 278 TETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF 292
           TETRTIARADVAEVC+QALQ EEAKFKALDLASKPEG GTPT DFKALF+QVTT F
Sbjct: 272 TETRTIARADVAEVCVQALQLEEAKFKALDLASKPEGTGTPTKDFKALFTQVTTKF 325

BLAST of Carg13220 vs. ExPASy Swiss-Prot
Match: Q94EG6 (Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.6e-80
Identity = 160/248 (64.52%), Postives = 181/248 (72.98%), Query Frame = 0

Query: 86  TVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLGP 145
           TVLVTGA GRTGQ+VYKKLKE SD++VA+GLVR+ + K+ IGG  D+FIGDI +AD++ P
Sbjct: 6   TVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEADVFIGDITDADSINP 65

Query: 146 AIQGIDALIILTSA------------------------------------------AAGV 205
           A QGIDAL+ILTSA                                           AGV
Sbjct: 66  AFQGIDALVILTSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIGQKNQIDAAKVAGV 125

Query: 206 KQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIR 265
           K IV+VGSMGGTN +HPLN LGNGNILVWKRKAEQYLADSG PYTIIRAGGL DK+GG+R
Sbjct: 126 KHIVVVGSMGGTNPDHPLNKLGNGNILVWKRKAEQYLADSGTPYTIIRAGGLLDKEGGVR 185

Query: 266 ELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKAL 292
           ELLVGKDDELLQT+T+T+ RADVAEVC+QAL FEEAK KA DL SKPEG  TPT DFKAL
Sbjct: 186 ELLVGKDDELLQTDTKTVPRADVAEVCIQALLFEEAKNKAFDLGSKPEGTSTPTKDFKAL 245

BLAST of Carg13220 vs. ExPASy Swiss-Prot
Match: D5JWB3 (Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.8e-69
Identity = 152/273 (55.68%), Postives = 173/273 (63.37%), Query Frame = 0

Query: 79  MADSGPR-TVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDI 138
           MADS  + TVL++GA G TG L +KKLKERSD++  RGLVR+E SKQ +GG D++FIGDI
Sbjct: 1   MADSSKKLTVLLSGASGLTGSLAFKKLKERSDKFEVRGLVRSEASKQKLGGGDEIFIGDI 60

Query: 139 RNADTLGPAIQGIDALIILTSA-------------------------------------- 198
            +  TL PA++GIDALIILTSA                                      
Sbjct: 61  SDPKTLEPAMEGIDALIILTSAIPRMKPTEEFTAEMISGGRSEDVIDASFSGPMPEFYYD 120

Query: 199 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 258
                                  GVK IVLVGSMGG + +H LN +GNGNIL+WKRKAEQ
Sbjct: 121 EGQYPEQVDWIGQKNQIDTAKKMGVKHIVLVGSMGGCDPDHFLNHMGNGNILIWKRKAEQ 180

Query: 259 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSGVPYTIIRAGGL +K GG+RELLV KDD LL TE   IARADVAE C+QAL+ EE
Sbjct: 181 YLADSGVPYTIIRAGGLDNKAGGVRELLVAKDDVLLPTENGFIARADVAEACVQALEIEE 240

BLAST of Carg13220 vs. ExPASy Swiss-Prot
Match: Q8H124 (Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g34460 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.9e-08
Identity = 68/241 (28.22%), Postives = 112/241 (46.47%), Query Frame = 0

Query: 85  RTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLG 144
           + V V GA G+TG+ + ++L  R    V  G+   E++K S    DD  +  +R   T G
Sbjct: 47  KKVFVAGATGQTGKRIVEQLLSRGFA-VKAGVRDVEKAKTSF--KDDPSLQIVRADVTEG 106

Query: 145 P----AIQGIDALIILTSAA---------------------------AGVKQIVLVGS-- 204
           P     + G D+  ++ +                              GV++ VLV S  
Sbjct: 107 PDKLAEVIGDDSQAVICATGFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSIL 166

Query: 205 MGGTNINHPLNSLG-----NGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELL 264
           + G  +   LN         G  LV K +AE+Y+  SG+ YTI+R GGL++ D     ++
Sbjct: 167 VNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKYIKKSGINYTIVRPGGLKN-DPPTGNVV 226

Query: 265 VGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQ 288
           +  +D L +    +I+R  VAEV ++AL  EE+ FK +++ ++ E    P   +K LF+ 
Sbjct: 227 MEPEDTLYE---GSISRDLVAEVAVEALLQEESSFKVVEIVARAE---APKRSYKDLFAS 277

BLAST of Carg13220 vs. ExPASy Swiss-Prot
Match: Q10A77 (Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 7.8e-06
Identity = 30/82 (36.59%), Postives = 46/82 (56.10%), Query Frame = 0

Query: 154 IILTSAAAGVKQIVLVGSMGGTNINHPLNSLG-NGNILVWKRKAEQYLADSGVPYTIIRA 213
           ++  + AA V+  +LV S+G   I  P   L     +L WKR+AE+ L  SG+PYTI+R 
Sbjct: 185 LVQAATAAKVEHFILVTSLGTNRIGFPAFLLNLFWGVLCWKRRAEEALIGSGLPYTIVRP 244

Query: 214 GGLQDKDGGIRE---LLVGKDD 232
           GG++      +E   L+V  +D
Sbjct: 245 GGMERPTDAFKETHNLVVAVED 266

BLAST of Carg13220 vs. ExPASy TrEMBL
Match: A0A6J1EZW5 (uncharacterized protein At5g02240 OS=Cucurbita moschata OX=3662 GN=LOC111438130 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 287/333 (86.19%), Postives = 288/333 (86.49%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSIS LS Q+VTSSISPT
Sbjct: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISSLSFQIVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE
Sbjct: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA                     
Sbjct: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQKNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. ExPASy TrEMBL
Match: A0A6J1HQY4 (uncharacterized protein At5g02240-like OS=Cucurbita maxima OX=3661 GN=LOC111465891 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 278/333 (83.48%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MKQSMAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPT 60
           MKQSMA LTRVPLAYSIP+PKSTLS P PFHQCHN CSLPNSNSISFLSLQ+VTSSISPT
Sbjct: 1   MKQSMATLTRVPLAYSIPSPKSTLSTPPPFHQCHNCCSLPNSNSISFLSLQIVTSSISPT 60

Query: 61  SQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTE 120
           SQK FRRR RVGALSLVAMADSG RTVLVTGAGGRTGQLVYKKLKERSD+YVARGLVRTE
Sbjct: 61  SQKSFRRRCRVGALSLVAMADSGSRTVLVTGAGGRTGQLVYKKLKERSDQYVARGLVRTE 120

Query: 121 ESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA--------------------- 180
           ESKQSIGGA+DLFIGDIRNADTLGPAIQGIDALIILTSA                     
Sbjct: 121 ESKQSIGGAEDLFIGDIRNADTLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFE 180

Query: 181 ---------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240
                                AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ
Sbjct: 181 DGAYPEQVDWIGQKNQIDTAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQ 240

Query: 241 YLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 292
           YLADSG+PYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE
Sbjct: 241 YLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEE 300

BLAST of Carg13220 vs. ExPASy TrEMBL
Match: A0A6J1ISE9 (uncharacterized protein At5g02240-like OS=Cucurbita maxima OX=3661 GN=LOC111478270 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.3e-124
Identity = 254/330 (76.97%), Postives = 264/330 (80.00%), Query Frame = 0

Query: 5   MAMLTRVPLAYSIPTPKSTL-SPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQK 64
           MAMLTRVPL Y+   PK+TL  PPL FHQCH  C +PNS+SI  LSL +V SSISPT  K
Sbjct: 1   MAMLTRVPLVYT--NPKATLFPPPLAFHQCHKCCFVPNSSSIPSLSLHIVASSISPTFHK 60

Query: 65  GFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESK 124
           GF RRRR GALS+VAMADSG  TVLVTGAGGRTGQLVYKKLKERSD+Y+ARGLVRTEESK
Sbjct: 61  GF-RRRRFGALSVVAMADSGLSTVLVTGAGGRTGQLVYKKLKERSDQYIARGLVRTEESK 120

Query: 125 QSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA------------------------ 184
           QSIG ADDLF+GDIR AD+LGPAIQGIDALIILTSA                        
Sbjct: 121 QSIGAADDLFVGDIRVADSLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFEDGA 180

Query: 185 ------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA 244
                             AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA
Sbjct: 181 YPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA 240

Query: 245 DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF 292
           DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF
Sbjct: 241 DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF 300

BLAST of Carg13220 vs. ExPASy TrEMBL
Match: A0A6J1GGP3 (uncharacterized protein At5g02240-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453737 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.6e-123
Identity = 253/330 (76.67%), Postives = 263/330 (79.70%), Query Frame = 0

Query: 5   MAMLTRVPLAYSIPTPKSTLSPP-LPFHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQK 64
           MAMLTRVPL Y+   PK+TL PP L FHQCH  C +PNS SI  LSL +V SSISPT  K
Sbjct: 1   MAMLTRVPLVYT--KPKATLFPPALAFHQCHKCCFVPNSTSIPSLSLHIVASSISPTFHK 60

Query: 65  GFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESK 124
           GF RRRR G+LS+VAMADSG  TVLVTGAGGRTGQLVYKKLKERSD+Y+ARGLVRTEESK
Sbjct: 61  GF-RRRRFGSLSVVAMADSGLSTVLVTGAGGRTGQLVYKKLKERSDQYIARGLVRTEESK 120

Query: 125 QSIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA------------------------ 184
           QSIG ADDLF+GDIR AD+LGPAIQGIDALIILTSA                        
Sbjct: 121 QSIGSADDLFVGDIRVADSLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFEDGA 180

Query: 185 ------------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA 244
                             AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA
Sbjct: 181 YPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLA 240

Query: 245 DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF 292
           DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF
Sbjct: 241 DSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKF 300

BLAST of Carg13220 vs. ExPASy TrEMBL
Match: A0A0A0KZL2 (NAD(P)-bd_dom domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G296130 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.1e-122
Identity = 250/329 (75.99%), Postives = 260/329 (79.03%), Query Frame = 0

Query: 5   MAMLTRVPLAYSIPTPKSTLSPPLPFHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQKG 64
           MAMLTRVPL YS    KST SPPLPFHQCH    + NSN I   SLQ++ SSIS TS KG
Sbjct: 1   MAMLTRVPLFYS--ASKSTSSPPLPFHQCHKCSFVTNSNLIPSSSLQILPSSISTTSHKG 60

Query: 65  FRRRRRVGALSLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQ 124
           F RRRR  ALS+VAMADS P TVLVTGAGGRTGQ+VYKKLKERSD Y ARGLVRTEESKQ
Sbjct: 61  F-RRRRFPALSVVAMADSAPPTVLVTGAGGRTGQIVYKKLKERSDHYAARGLVRTEESKQ 120

Query: 125 SIGGADDLFIGDIRNADTLGPAIQGIDALIILTSA------------------------- 184
           SIGGADDLF+GDIR+AD+LGPAIQGIDALIILTSA                         
Sbjct: 121 SIGGADDLFVGDIRDADSLGPAIQGIDALIILTSAVPKMKPGFDPTKGGRPEFYFEDGAY 180

Query: 185 -----------------AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLAD 244
                            AAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLAD
Sbjct: 181 PEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLAD 240

Query: 245 SGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFK 292
           SG+PYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVC+QALQFEEAKFK
Sbjct: 241 SGIPYTIIRAGGLQDKDGGIRELLVGKDDELLQTETRTIARADVAEVCIQALQFEEAKFK 300

BLAST of Carg13220 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 348.6 bits (893), Expect = 4.9e-96
Identity = 191/296 (64.53%), Postives = 220/296 (74.32%), Query Frame = 0

Query: 38  SLPNSNSISFLSLQLVTSSISPTSQKGFRRRRRVGALSLVAMADSGPRTVLVTGAGGRTG 97
           S+P S+S+ F S  LV+ S S      F  + R  ++++ A A + P TVLVTGAGGRTG
Sbjct: 32  SVPRSSSLQFRS--LVSDSTSICGPSKFTGKNRRVSVTVSAAATTEPLTVLVTGAGGRTG 91

Query: 98  QLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLGPAIQGIDALIILT 157
           Q+VYKKLKERS+++VARGLVRT+ESK+ I G D++FIGDIR+  ++ PA++GIDAL+ILT
Sbjct: 92  QIVYKKLKERSEQFVARGLVRTKESKEKINGEDEVFIGDIRDTASIAPAVEGIDALVILT 151

Query: 158 SA------------------------------------------AAGVKQIVLVGSMGGT 217
           SA                                          AAGVKQIVLVGSMGGT
Sbjct: 152 SAVPQMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGT 211

Query: 218 NINHPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELLVGKDDELLQ 277
           NINHPLNS+GN NILVWKRKAEQYLADSG+PYTIIRAGGLQDKDGGIRELLVGKDDELL+
Sbjct: 212 NINHPLNSIGNANILVWKRKAEQYLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDELLE 271

Query: 278 TETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQVTTSF 292
           TETRTIARADVAEVC+QALQ EEAKFKALDLASKPEG GTPT DFKALF+QVTT F
Sbjct: 272 TETRTIARADVAEVCVQALQLEEAKFKALDLASKPEGTGTPTKDFKALFTQVTTKF 325

BLAST of Carg13220 vs. TAIR 10
Match: AT5G02240.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.2e-81
Identity = 160/248 (64.52%), Postives = 181/248 (72.98%), Query Frame = 0

Query: 86  TVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLGP 145
           TVLVTGA GRTGQ+VYKKLKE SD++VA+GLVR+ + K+ IGG  D+FIGDI +AD++ P
Sbjct: 6   TVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEADVFIGDITDADSINP 65

Query: 146 AIQGIDALIILTSA------------------------------------------AAGV 205
           A QGIDAL+ILTSA                                           AGV
Sbjct: 66  AFQGIDALVILTSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIGQKNQIDAAKVAGV 125

Query: 206 KQIVLVGSMGGTNINHPLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIR 265
           K IV+VGSMGGTN +HPLN LGNGNILVWKRKAEQYLADSG PYTIIRAGGL DK+GG+R
Sbjct: 126 KHIVVVGSMGGTNPDHPLNKLGNGNILVWKRKAEQYLADSGTPYTIIRAGGLLDKEGGVR 185

Query: 266 ELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKAL 292
           ELLVGKDDELLQT+T+T+ RADVAEVC+QAL FEEAK KA DL SKPEG  TPT DFKAL
Sbjct: 186 ELLVGKDDELLQTDTKTVPRADVAEVCIQALLFEEAKNKAFDLGSKPEGTSTPTKDFKAL 245

BLAST of Carg13220 vs. TAIR 10
Match: AT4G31530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-15
Identity = 92/321 (28.66%), Postives = 134/321 (41.74%), Query Frame = 0

Query: 17  IPTPKSTLSPPLP--FHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQKGFRRRRRVGAL 76
           I  P  T   PLP  F    N  SL    S S   +Q  T     TS             
Sbjct: 24  IRNPLFTSPLPLPSSFFLVRNEASL----SSSITPVQAFTEEAVDTSD------------ 83

Query: 77  SLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFI 136
               +A S  + VLV G  G  GQLV   L +R+    +R L+R  +    + G  D + 
Sbjct: 84  ----LASSSSKLVLVVGGTGGVGQLVVASLLKRNIR--SRLLLRDLDKATKLFGKQDEYS 143

Query: 137 -----GDIRNADTLGPAI-QGIDALIILTSAAA--------------------------- 196
                GD RNA+ L P++ +G+  +I  T   A                           
Sbjct: 144 LQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISAL 203

Query: 197 --GVKQIVLVGSMGGTNINH-PLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQD- 256
              VK++VLV S+G T  N  P + +    +L +K+  E +L DSG+P+TIIR G L D 
Sbjct: 204 PSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFLRDSGLPFTIIRPGRLTDG 263

Query: 257 -------------KDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKAL 285
                          G  R +++G+ D L+   +R +    VAE C+QAL  E  + KA 
Sbjct: 264 PYTSYDLNTLLKATAGERRAVVIGQGDNLVGEVSRLV----VAEACIQALDIEFTQGKAY 318

BLAST of Carg13220 vs. TAIR 10
Match: AT4G31530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 6.1e-14
Identity = 87/304 (28.62%), Postives = 125/304 (41.12%), Query Frame = 0

Query: 17  IPTPKSTLSPPLP--FHQCHNRCSLPNSNSISFLSLQLVTSSISPTSQKGFRRRRRVGAL 76
           I  P  T   PLP  F    N  SL    S S   +Q  T     TS             
Sbjct: 24  IRNPLFTSPLPLPSSFFLVRNEASL----SSSITPVQAFTEEAVDTSD------------ 83

Query: 77  SLVAMADSGPRTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFI 136
               +A S  + VLV G  G  GQLV   L +R+    +R L+R  +    + G  D + 
Sbjct: 84  ----LASSSSKLVLVVGGTGGVGQLVVASLLKRNIR--SRLLLRDLDKATKLFGKQDEYS 143

Query: 137 -----GDIRNADTLGPAI-QGIDALIILTSAAA--------------------------- 196
                GD RNA+ L P++ +G+  +I  T   A                           
Sbjct: 144 LQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISAL 203

Query: 197 --GVKQIVLVGSMGGTNINH-PLNSLGNGNILVWKRKAEQYLADSGVPYTIIRAGGLQD- 256
              VK++VLV S+G T  N  P + +    +L +K+  E +L DSG+P+TIIR G L D 
Sbjct: 204 PSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFLRDSGLPFTIIRPGRLTDG 263

Query: 257 -------------KDGGIRELLVGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKAL 269
                          G  R +++G+ D L+   +R +    VAE C+QAL  E  + KA 
Sbjct: 264 PYTSYDLNTLLKATAGERRAVVIGQGDNLVGEVSRLV----VAEACIQALDIEFTQGKAY 301

BLAST of Carg13220 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-09
Identity = 68/241 (28.22%), Postives = 112/241 (46.47%), Query Frame = 0

Query: 85  RTVLVTGAGGRTGQLVYKKLKERSDEYVARGLVRTEESKQSIGGADDLFIGDIRNADTLG 144
           + V V GA G+TG+ + ++L  R    V  G+   E++K S    DD  +  +R   T G
Sbjct: 47  KKVFVAGATGQTGKRIVEQLLSRGFA-VKAGVRDVEKAKTSF--KDDPSLQIVRADVTEG 106

Query: 145 P----AIQGIDALIILTSAA---------------------------AGVKQIVLVGS-- 204
           P     + G D+  ++ +                              GV++ VLV S  
Sbjct: 107 PDKLAEVIGDDSQAVICATGFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSIL 166

Query: 205 MGGTNINHPLNSLG-----NGNILVWKRKAEQYLADSGVPYTIIRAGGLQDKDGGIRELL 264
           + G  +   LN         G  LV K +AE+Y+  SG+ YTI+R GGL++ D     ++
Sbjct: 167 VNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKYIKKSGINYTIVRPGGLKN-DPPTGNVV 226

Query: 265 VGKDDELLQTETRTIARADVAEVCLQALQFEEAKFKALDLASKPEGVGTPTTDFKALFSQ 288
           +  +D L +    +I+R  VAEV ++AL  EE+ FK +++ ++ E    P   +K LF+ 
Sbjct: 227 MEPEDTLYE---GSISRDLVAEVAVEALLQEESSFKVVEIVARAE---APKRSYKDLFAS 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027340.11.1e-155100.00putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6595330.14.7e-14987.09putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022931843.12.6e-14786.19uncharacterized protein At5g02240 [Cucurbita moschata][more]
XP_023518926.12.2e-14685.59uncharacterized protein At5g02240-like [Cucurbita pepo subsp. pepo][more]
XP_022966108.12.1e-14183.48uncharacterized protein At5g02240-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O809346.9e-9564.53Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q94EG61.6e-8064.52Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 P... [more]
D5JWB33.8e-6955.68Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1[more]
Q8H1242.9e-0828.22Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q10A777.8e-0636.59Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 ... [more]
Match NameE-valueIdentityDescription
A0A6J1EZW51.2e-14786.19uncharacterized protein At5g02240 OS=Cucurbita moschata OX=3662 GN=LOC111438130 ... [more]
A0A6J1HQY41.0e-14183.48uncharacterized protein At5g02240-like OS=Cucurbita maxima OX=3661 GN=LOC1114658... [more]
A0A6J1ISE93.3e-12476.97uncharacterized protein At5g02240-like OS=Cucurbita maxima OX=3661 GN=LOC1114782... [more]
A0A6J1GGP31.6e-12376.67uncharacterized protein At5g02240-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0KZL23.1e-12275.99NAD(P)-bd_dom domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G2961... [more]
Match NameE-valueIdentityDescription
AT2G37660.14.9e-9664.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02240.11.2e-8164.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.11.9e-1528.66NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.26.1e-1428.62NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G34460.12.0e-0928.22NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 160..291
e-value: 8.5E-40
score: 138.8
coord: 84..159
e-value: 1.2E-18
score: 69.5
NoneNo IPR availablePANTHERPTHR14194:SF103NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 160..291
NoneNo IPR availablePANTHERPTHR14194:SF103NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 52..159
NoneNo IPR availableCDDcd05243SDR_a5coord: 86..266
e-value: 2.60164E-47
score: 155.471
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 91..157
e-value: 1.4E-7
score: 31.5
coord: 158..255
e-value: 2.9E-17
score: 63.1
IPR044163Sanguinarine reductase SARED1-likePANTHERPTHR14194NITROGEN METABOLIC REGULATION PROTEIN NMR-RELATEDcoord: 160..291
IPR044163Sanguinarine reductase SARED1-likePANTHERPTHR14194NITROGEN METABOLIC REGULATION PROTEIN NMR-RELATEDcoord: 52..159
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 85..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13220-RACarg13220-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity