Carg13147 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13147
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein MODIFYING WALL LIGNIN-2-like
LocationCarg_Chr04: 3166940 .. 3168006 (+)
RNA-Seq ExpressionCarg13147
SyntenyCarg13147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAACGCCGTTTCGTCTACGCCTTAAGCCTCTCGATCGTCGTCTCTTTCGCACTCGTTGCCATTGTGTCGTGTATAGCTGCAGAATTACACAGAACAAAGGTATAGAATTCATCGCACAAGTGTGTGTTTGTTCATTAAGAACTTGGTTTTTGGCTAACTTTTCCCTCTTTTTTTTGTAGACCAAAGATCTCAGATTGGATGGGAAACTGTGTTATTTGCCAGAAAGTCAAGCATTTAGATATGGAGTTGCAGCATTGGCGTGTTTGGTTATTGCTCAAGTTATTGGAAATGTCTTGTTCTGCACAAGTTGCAGTTTGAACTCAAGAGGGAAGAAAAGCAATGATCAACAACCTCCTAGAAGATCAAATTTGGCCATAATTCTTCTTGTTATTTCTTGGTAGGTTTGGTTCATTTCTCTTGTTTTAACTGTTTGATCAGCCTTCCAAAAGTCATTCAAATCACCAATCAATAGTCATAAATCTTCAATTTCAAGAACTCATCATCTAGTCTCATTCATGTTTATGTTCAATTTCTTGAGAATTTGCTAATAATTCATTCTTCTGATTCAGAATCTTCAAAGAAATTTGTAAGAATTTGCTAATAGTTGATTATTCATGAAACGGAAAAATCAATTCAAATCGAAAATGTAATTCAAGAGGAGATTTGATGTTCTAGGGCAAGCTTTACCGTGGTGATATTGCTACTGAGCGCGGCGACGAGTATGAGTAGACAGCAACAATACGGTGCGGGCTGGTTGAGCGGCGAGTGCTATTTGGTGAAGAGCGGCGTATACATCGCCGCAGCAATTCTGATCCTCGTTTCGACATGTTCAATCGTCTGCTCCGCCGTAGCAGTCTTGAGGAAGAGCCTTCAGATCGACGAATCCAGAAAAACTAGTACACTTCCAAAATAAAAAAAAAGGAAATAAAAGCGTCGCAACGGGAACGGCAACGGCGGCGATTGGAAGAAGGCGGCGAAGCGCCATTGACGAGAGCTCCACGGATGCCATGTGTCCTGTGGATGGCGCCTTCACTGGAACGGTCTCTTGGATCTCCAGCTAA

mRNA sequence

ATGGAAAAACGCCGTTTCGTCTACGCCTTAAGCCTCTCGATCGTCGTCTCTTTCGCACTCGTTGCCATTGTGTCGTGTATAGCTGCAGAATTACACAGAACAAAGACCAAAGATCTCAGATTGGATGGGAAACTGTGTTATTTGCCAGAAAGTCAAGCATTTAGATATGGAGTTGCAGCATTGGCGTGTTTGGTTATTGCTCAAGTTATTGGAAATGTCTTGTTCTGCACAAGTTGCAGTTTGAACTCAAGAGGGAAGAAAAGCAATGATCAACAACCTCCTAGAAGATCAAATTTGGCCATAATTCTTCTTGTTATTTCTTGGGCAAGCTTTACCGTGGTGATATTGCTACTGAGCGCGGCGACGAGTATGAGTAGACAGCAACAATACGGTGCGGGCTGGTTGAGCGGCGAGTGCTATTTGGTGAAGAGCGGCGTATACATCGCCGCAGCAATTCTGATCCTCGTTTCGACATGTTCAATCGTCTGCTCCGCCGTAGCAGTCTTGAGGAAGAGCCTTCAGATCGACGAATCCAGAAAAACTAGAAATAAAAGCGTCGCAACGGGAACGGCAACGGCGGCGATTGGAAGAAGGCGGCGAAGCGCCATTGACGAGAGCTCCACGGATGCCATGTGTCCTGTGGATGGCGCCTTCACTGGAACGGTCTCTTGGATCTCCAGCTAA

Coding sequence (CDS)

ATGGAAAAACGCCGTTTCGTCTACGCCTTAAGCCTCTCGATCGTCGTCTCTTTCGCACTCGTTGCCATTGTGTCGTGTATAGCTGCAGAATTACACAGAACAAAGACCAAAGATCTCAGATTGGATGGGAAACTGTGTTATTTGCCAGAAAGTCAAGCATTTAGATATGGAGTTGCAGCATTGGCGTGTTTGGTTATTGCTCAAGTTATTGGAAATGTCTTGTTCTGCACAAGTTGCAGTTTGAACTCAAGAGGGAAGAAAAGCAATGATCAACAACCTCCTAGAAGATCAAATTTGGCCATAATTCTTCTTGTTATTTCTTGGGCAAGCTTTACCGTGGTGATATTGCTACTGAGCGCGGCGACGAGTATGAGTAGACAGCAACAATACGGTGCGGGCTGGTTGAGCGGCGAGTGCTATTTGGTGAAGAGCGGCGTATACATCGCCGCAGCAATTCTGATCCTCGTTTCGACATGTTCAATCGTCTGCTCCGCCGTAGCAGTCTTGAGGAAGAGCCTTCAGATCGACGAATCCAGAAAAACTAGAAATAAAAGCGTCGCAACGGGAACGGCAACGGCGGCGATTGGAAGAAGGCGGCGAAGCGCCATTGACGAGAGCTCCACGGATGCCATGTGTCCTGTGGATGGCGCCTTCACTGGAACGGTCTCTTGGATCTCCAGCTAA

Protein sequence

MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAALACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSAATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRKTRNKSVATGTATAAIGRRRRSAIDESSTDAMCPVDGAFTGTVSWISS
Homology
BLAST of Carg13147 vs. NCBI nr
Match: KAG7031081.1 (hypothetical protein SDJN02_05120, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.2 bits (1084), Expect = 2.9e-114
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA
Sbjct: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180

Query: 181 TRNKSVATGTATAAIGRRRRSAIDESSTDAMCPVDGAFTGTVSWISS 228
           TRNKSVATGTATAAIGRRRRSAIDESSTDAMCPVDGAFTGTVSWISS
Sbjct: 181 TRNKSVATGTATAAIGRRRRSAIDESSTDAMCPVDGAFTGTVSWISS 227

BLAST of Carg13147 vs. NCBI nr
Match: KAG6600430.1 (Protein MODIFYING WALL LIGNIN-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.4 bits (864), Expect = 9.4e-89
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA
Sbjct: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. NCBI nr
Match: XP_023543619.1 (uncharacterized protein LOC111803449 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.0 bits (855), Expect = 1.0e-87
Identity = 180/181 (99.45%), Postives = 180/181 (99.45%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRS LAIILLVISWASFTVVILLLSA
Sbjct: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSILAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. NCBI nr
Match: XP_022941598.1 (uncharacterized protein LOC111446907 [Cucurbita moschata])

HSP 1 Score: 330.5 bits (846), Expect = 1.2e-86
Identity = 179/181 (98.90%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQ PRRS LAIILLVISWASFTVVILLLSA
Sbjct: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQRPRRSILAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. NCBI nr
Match: XP_022982923.1 (uncharacterized protein LOC111481621 [Cucurbita maxima])

HSP 1 Score: 325.5 bits (833), Expect = 3.7e-85
Identity = 176/181 (97.24%), Postives = 178/181 (98.34%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LAC VIAQVIGNVLFCT+CSLNSRGKK+NDQQPPRRS LAIILLVISWASFTVVILLLSA
Sbjct: 61  LACFVIAQVIGNVLFCTNCSLNSRGKKNNDQQPPRRSILAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVST SIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTGSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. ExPASy Swiss-Prot
Match: A2RVU1 (Protein MODIFYING WALL LIGNIN-1 OS=Arabidopsis thaliana OX=3702 GN=MWL1 PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-26
Identity = 69/179 (38.55%), Postives = 104/179 (58.10%), Query Frame = 0

Query: 18  FALVAIVSCIAAELHRTKT----------KDLRLDGKLCYLPESQAFRYGVAALACLVIA 77
           F L A   C++AE  + K           KDL+ DG+ CYLPE++AF  G+AAL C+ +A
Sbjct: 6   FGLAAFFLCLSAEFQKAKALLRAQVFLKGKDLKWDGESCYLPENRAFGLGIAALVCVSVA 65

Query: 78  QVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSAATSMSRQ 137
           Q++GNV+ C       RG    D+   R +   IILL+ SW +F V + L+S   SM+R+
Sbjct: 66  QIVGNVVIC-------RGFTKTDK--TRTTIFCIILLLFSWVNFAVAVTLISVGASMNRE 125

Query: 138 QQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRKTRNKSV 187
           Q YG GWL+ ECYLVK GV+ A+  L + +  +I+ +    ++ SLQ++   K   ++V
Sbjct: 126 QIYGKGWLNRECYLVKDGVFAASGFLSVTTMAAILGAFAFKVKPSLQVENHDKRHTQNV 175

BLAST of Carg13147 vs. ExPASy Swiss-Prot
Match: O65708 (Protein MODIFYING WALL LIGNIN-2 OS=Arabidopsis thaliana OX=3702 GN=MWL2 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.8e-24
Identity = 65/163 (39.88%), Postives = 97/163 (59.51%), Query Frame = 0

Query: 13  SIVVSFALVAIVSCIAAELHRTKTKDLRLD-GKLCYLPESQAFRYGVAALACLVIAQVIG 72
           S+V S  LV+ ++C AAE  RT+ +D+R D  + CY+P S AF  G AA+ C  +AQ++G
Sbjct: 8   SVVFSLGLVSFITCFAAEFKRTQKEDIRWDTERNCYVPGSHAFGLGSAAVLCFCLAQIVG 67

Query: 73  NVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSAATSMSRQQQYG 132
           N++   +   +    K  D        L  +LL++SW++F VV+L+LS A SMSR Q YG
Sbjct: 68  NIVVFRN---HRTRTKREDGYKITDLTLPTVLLLLSWSNFVVVVLILSTAISMSRAQAYG 127

Query: 133 AGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQ 175
            GWL  +CYLVK GV+ A+  L ++   ++  SA  +  K  Q
Sbjct: 128 EGWLDEDCYLVKDGVFAASGCLAILGLGALTISATRIKVKKQQ 167

BLAST of Carg13147 vs. ExPASy TrEMBL
Match: A0A6J1FSJ4 (uncharacterized protein LOC111446907 OS=Cucurbita moschata OX=3662 GN=LOC111446907 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.6e-87
Identity = 179/181 (98.90%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQ PRRS LAIILLVISWASFTVVILLLSA
Sbjct: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQRPRRSILAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. ExPASy TrEMBL
Match: A0A6J1J6B1 (uncharacterized protein LOC111481621 OS=Cucurbita maxima OX=3661 GN=LOC111481621 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.8e-85
Identity = 176/181 (97.24%), Postives = 178/181 (98.34%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA
Sbjct: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LAC VIAQVIGNVLFCT+CSLNSRGKK+NDQQPPRRS LAIILLVISWASFTVVILLLSA
Sbjct: 61  LACFVIAQVIGNVLFCTNCSLNSRGKKNNDQQPPRRSILAIILLVISWASFTVVILLLSA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVST SIVCSAVAVLRKSLQIDESRK
Sbjct: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTGSIVCSAVAVLRKSLQIDESRK 180

Query: 181 T 182
           T
Sbjct: 181 T 181

BLAST of Carg13147 vs. ExPASy TrEMBL
Match: A0A0A0KYL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056580 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.5e-63
Identity = 137/181 (75.69%), Postives = 152/181 (83.98%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           M K RF YALSLSIVVS AL+A VSC+AAELHRTKTKDL+LDGKLCYLPESQAF YGVAA
Sbjct: 1   MGKHRFGYALSLSIVVSLALIAFVSCVAAELHRTKTKDLKLDGKLCYLPESQAFGYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLV+AQVIGN+L CTSCS+NSR KK   +QPP+R NLA   LV+SWASFTVVILLLS 
Sbjct: 61  LACLVMAQVIGNILLCTSCSINSREKK-RSEQPPKRPNLATFFLVVSWASFTVVILLLST 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           A+SMSRQQ Y  GWL GECYLVKSGVY+AAAILIL+S CS V SAV V     +I+ESRK
Sbjct: 121 ASSMSRQQPYATGWLGGECYLVKSGVYVAAAILILISICSTVGSAVTV-----RINESRK 175

Query: 181 T 182
           +
Sbjct: 181 S 175

BLAST of Carg13147 vs. ExPASy TrEMBL
Match: A0A5A7T9R1 (DUF1218 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold118G00180 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.8e-61
Identity = 134/181 (74.03%), Postives = 151/181 (83.43%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           M K  F YALSLSIVVS AL+A VSC+AAELHRTKTKDL+LDGKLCYLPESQAF YGVAA
Sbjct: 1   MGKHCFGYALSLSIVVSLALIAFVSCVAAELHRTKTKDLKLDGKLCYLPESQAFGYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLV+AQVIGN+L CTSCS+NSR KK   +QPP+R NLA   LV+SWASFTVVILLL A
Sbjct: 61  LACLVMAQVIGNILLCTSCSINSREKK-RSEQPPKRPNLATFFLVVSWASFTVVILLLIA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           A+SMSRQQ Y  GWL GECYLVKSGVY+AAAILIL+S C+ V SAV V     +++ESRK
Sbjct: 121 ASSMSRQQPYATGWLGGECYLVKSGVYVAAAILILISICTTVGSAVTV-----RMNESRK 175

Query: 181 T 182
           +
Sbjct: 181 S 175

BLAST of Carg13147 vs. ExPASy TrEMBL
Match: A0A1S3BTM2 (uncharacterized protein LOC103493027 OS=Cucumis melo OX=3656 GN=LOC103493027 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.8e-61
Identity = 134/181 (74.03%), Postives = 151/181 (83.43%), Query Frame = 0

Query: 1   MEKRRFVYALSLSIVVSFALVAIVSCIAAELHRTKTKDLRLDGKLCYLPESQAFRYGVAA 60
           M K  F YALSLSIVVS AL+A VSC+AAELHRTKTKDL+LDGKLCYLPESQAF YGVAA
Sbjct: 1   MGKHCFGYALSLSIVVSLALIAFVSCVAAELHRTKTKDLKLDGKLCYLPESQAFGYGVAA 60

Query: 61  LACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSA 120
           LACLV+AQVIGN+L CTSCS+NSR KK   +QPP+R NLA   LV+SWASFTVVILLL A
Sbjct: 61  LACLVMAQVIGNILLCTSCSINSREKK-RSEQPPKRPNLATFFLVVSWASFTVVILLLIA 120

Query: 121 ATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESRK 180
           A+SMSRQQ Y  GWL GECYLVKSGVY+AAAILIL+S C+ V SAV V     +++ESRK
Sbjct: 121 ASSMSRQQPYATGWLGGECYLVKSGVYVAAAILILISICTTVGSAVTV-----RMNESRK 175

Query: 181 T 182
           +
Sbjct: 181 S 175

BLAST of Carg13147 vs. TAIR 10
Match: AT1G31720.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 119.4 bits (298), Expect = 3.8e-27
Identity = 70/187 (37.43%), Postives = 106/187 (56.68%), Query Frame = 0

Query: 10  LSLSIVVSFALVAIVSCIAAELHRTKT----------KDLRLDGKLCYLPESQAFRYGVA 69
           L    +  F L A   C++AE  + K           KDL+ DG+ CYLPE++AF  G+A
Sbjct: 17  LFFMFIFLFGLAAFFLCLSAEFQKAKALLRAQVFLKGKDLKWDGESCYLPENRAFGLGIA 76

Query: 70  ALACLVIAQVIGNVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLS 129
           AL C+ +AQ++GNV+ C       RG    D+   R +   IILL+ SW +F V + L+S
Sbjct: 77  ALVCVSVAQIVGNVVIC-------RGFTKTDK--TRTTIFCIILLLFSWVNFAVAVTLIS 136

Query: 130 AATSMSRQQQYGAGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQIDESR 187
              SM+R+Q YG GWL+ ECYLVK GV+ A+  L + +  +I+ +    ++ SLQ++   
Sbjct: 137 VGASMNREQIYGKGWLNRECYLVKDGVFAASGFLSVTTMAAILGAFAFKVKPSLQVENHD 194

BLAST of Carg13147 vs. TAIR 10
Match: AT4G19370.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-25
Identity = 65/163 (39.88%), Postives = 97/163 (59.51%), Query Frame = 0

Query: 13  SIVVSFALVAIVSCIAAELHRTKTKDLRLD-GKLCYLPESQAFRYGVAALACLVIAQVIG 72
           S+V S  LV+ ++C AAE  RT+ +D+R D  + CY+P S AF  G AA+ C  +AQ++G
Sbjct: 8   SVVFSLGLVSFITCFAAEFKRTQKEDIRWDTERNCYVPGSHAFGLGSAAVLCFCLAQIVG 67

Query: 73  NVLFCTSCSLNSRGKKSNDQQPPRRSNLAIILLVISWASFTVVILLLSAATSMSRQQQYG 132
           N++   +   +    K  D        L  +LL++SW++F VV+L+LS A SMSR Q YG
Sbjct: 68  NIVVFRN---HRTRTKREDGYKITDLTLPTVLLLLSWSNFVVVVLILSTAISMSRAQAYG 127

Query: 133 AGWLSGECYLVKSGVYIAAAILILVSTCSIVCSAVAVLRKSLQ 175
            GWL  +CYLVK GV+ A+  L ++   ++  SA  +  K  Q
Sbjct: 128 EGWLDEDCYLVKDGVFAASGCLAILGLGALTISATRIKVKKQQ 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031081.12.9e-114100.00hypothetical protein SDJN02_05120, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6600430.19.4e-89100.00Protein MODIFYING WALL LIGNIN-1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023543619.11.0e-8799.45uncharacterized protein LOC111803449 [Cucurbita pepo subsp. pepo][more]
XP_022941598.11.2e-8698.90uncharacterized protein LOC111446907 [Cucurbita moschata][more]
XP_022982923.13.7e-8597.24uncharacterized protein LOC111481621 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A2RVU12.4e-2638.55Protein MODIFYING WALL LIGNIN-1 OS=Arabidopsis thaliana OX=3702 GN=MWL1 PE=1 SV=... [more]
O657083.8e-2439.88Protein MODIFYING WALL LIGNIN-2 OS=Arabidopsis thaliana OX=3702 GN=MWL2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1FSJ45.6e-8798.90uncharacterized protein LOC111446907 OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
A0A6J1J6B11.8e-8597.24uncharacterized protein LOC111481621 OS=Cucurbita maxima OX=3661 GN=LOC111481621... [more]
A0A0A0KYL92.5e-6375.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056580 PE=4 SV=1[more]
A0A5A7T9R11.8e-6174.03DUF1218 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3BTM21.8e-6174.03uncharacterized protein LOC103493027 OS=Cucumis melo OX=3656 GN=LOC103493027 PE=... [more]
Match NameE-valueIdentityDescription
AT1G31720.13.8e-2737.43Protein of unknown function (DUF1218) [more]
AT4G19370.12.7e-2539.88Protein of unknown function (DUF1218) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009606Modifying wall lignin-1/2PFAMPF06749DUF1218coord: 60..158
e-value: 1.9E-16
score: 60.3
NoneNo IPR availablePANTHERPTHR31769:SF6PROTEIN MODIFYING WALL LIGNIN-1coord: 2..197
NoneNo IPR availablePANTHERPTHR31769OS07G0462200 PROTEIN-RELATEDcoord: 2..197
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..26
score: 7.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13147-RACarg13147-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane