Carg13109 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13109
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionserine/threonine-protein kinase WAG1-like
LocationCarg_Chr04: 3400951 .. 3402303 (+)
RNA-Seq ExpressionCarg13109
SyntenyCarg13109
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGACGATTCAGATCTTGACTTCAGCTTCACCAGCACCATCTCCACCTCCACCGCCCGCAGCAGTTTAACTCTCAGCTTTAACGAATCCCGTGGCTTCTCCGCCCTTAACCTCCGTCCCCACCGCCACTCCGACTCACACTGGTCCGCCATCAAAGCTGCCACTACTCTTTCCTCCGATGGCCACCTCCACCTCCGCCACCTGAAACTCATCAAACACCTCGGCACTGGCAACCTCGGTCGGGTTTTCCTCTGCCATTTGAGAGACAACGATCACGCCAATTTCGCCCTTAAGGTCGTTGACAGAGACGCTTTAAGTAACAAGAAGCTTTTGCAGGTTCAAACGGAATCCGAGATTCTCGCTTTGCTCGACAACCCTTTTCTCCCTACTCTCTACGCGCGTCTCGACGTCTCTCACTACACGTGCCTCCTTATTGACTACTGCCCAGCCGGTGACCTCCACTCTCTCCTCCGCAAACAGCCTGGAAACCGCCTCTCCATTTCGGCGGCGAGATTCTTCGTCGCTGAGGTTCTGGTGGCGCTTGAGTATCTTCACGCGCTTGGGATTGTGTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCACTGATTTCGATTTGTGTTTCAAGTCCGACGTTGTCCCCACTTTTCACACGTCGACACAGCGGGGACCACAGGGTACTGCCTCCTGCTTTGGGATGAGGACCGCGGCGGAGGAGATTGTCGGGGAGTTCGTGGCGGAGCCGACGACGGCCTTTTCGAAGTCATGTGTAGGGACTCATGAGTATTTGGCGCCTGAGTTGGTAACCGGCGGTGGCCACGGAAACGCCGTCGACTGGTGGGCGTTTGGGGTGTTCATTTACGAGCTGCTACACGGAACGACGCCGTTTAAGGGAGTGAACAAGGAGGGTACGCTTAGAAACATAGCGTCTACAGAAGGGGTGAAGTTCAGGGCGGCCATGGAGGAAGAGGAAGGTATAGCAGAGGCGAGGGATCTGATAGAGAGACTACTGGTGAAAGATCCGGCGAGACGGTTGGGATCCGGGAAAGGGGCGACGGAGATCAAACGGCATCCATTCTTCAACGGTATAAAATGGGCGTTAATCCGGATGTACCGACCGCCGGAGGTGTACGGATTGATGAGAAAAGCCAGATCACACGTGAGCCACGTGCGTGACGTCAACCACCGCATGCGGCGTCGGAGCTGGTGGAACTGGAGAAAGCTCAGTAACTATTTAATTAAAAACGGAAATTCCAGTAAATCTCATACCAATACCAATTCCAATACTAATAATTACAGATTTAACGCCAAAAATAAAATGGCTTAA

mRNA sequence

ATGGAGGACGATTCAGATCTTGACTTCAGCTTCACCAGCACCATCTCCACCTCCACCGCCCGCAGCAGTTTAACTCTCAGCTTTAACGAATCCCGTGGCTTCTCCGCCCTTAACCTCCGTCCCCACCGCCACTCCGACTCACACTGGTCCGCCATCAAAGCTGCCACTACTCTTTCCTCCGATGGCCACCTCCACCTCCGCCACCTGAAACTCATCAAACACCTCGGCACTGGCAACCTCGGTCGGGTTTTCCTCTGCCATTTGAGAGACAACGATCACGCCAATTTCGCCCTTAAGGTCGTTGACAGAGACGCTTTAAGTAACAAGAAGCTTTTGCAGGTTCAAACGGAATCCGAGATTCTCGCTTTGCTCGACAACCCTTTTCTCCCTACTCTCTACGCGCGTCTCGACGTCTCTCACTACACGTGCCTCCTTATTGACTACTGCCCAGCCGGTGACCTCCACTCTCTCCTCCGCAAACAGCCTGGAAACCGCCTCTCCATTTCGGCGGCGAGATTCTTCGTCGCTGAGGTTCTGGTGGCGCTTGAGTATCTTCACGCGCTTGGGATTGTGTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCACTGATTTCGATTTGTGTTTCAAGTCCGACGTTGTCCCCACTTTTCACACGTCGACACAGCGGGGACCACAGGGTACTGCCTCCTGCTTTGGGATGAGGACCGCGGCGGAGGAGATTGTCGGGGAGTTCGTGGCGGAGCCGACGACGGCCTTTTCGAAGTCATGTGTAGGGACTCATGAGTATTTGGCGCCTGAGTTGGTAACCGGCGGTGGCCACGGAAACGCCGTCGACTGGTGGGCGTTTGGGGTGTTCATTTACGAGCTGCTACACGGAACGACGCCGTTTAAGGGAGTGAACAAGGAGGGTACGCTTAGAAACATAGCGTCTACAGAAGGGGTGAAGTTCAGGGCGGCCATGGAGGAAGAGGAAGGTATAGCAGAGGCGAGGGATCTGATAGAGAGACTACTGGTGAAAGATCCGGCGAGACGGTTGGGATCCGGGAAAGGGGCGACGGAGATCAAACGGCATCCATTCTTCAACGGTATAAAATGGGCGTTAATCCGGATGTACCGACCGCCGGAGGTGTACGGATTGATGAGAAAAGCCAGATCACACGTGAGCCACGTGCGTGACGTCAACCACCGCATGCGGCGTCGGAGCTGGTGGAACTGGAGAAAGCTCAGTAACTATTTAATTAAAAACGGAAATTCCAGTAAATCTCATACCAATACCAATTCCAATACTAATAATTACAGATTTAACGCCAAAAATAAAATGGCTTAA

Coding sequence (CDS)

ATGGAGGACGATTCAGATCTTGACTTCAGCTTCACCAGCACCATCTCCACCTCCACCGCCCGCAGCAGTTTAACTCTCAGCTTTAACGAATCCCGTGGCTTCTCCGCCCTTAACCTCCGTCCCCACCGCCACTCCGACTCACACTGGTCCGCCATCAAAGCTGCCACTACTCTTTCCTCCGATGGCCACCTCCACCTCCGCCACCTGAAACTCATCAAACACCTCGGCACTGGCAACCTCGGTCGGGTTTTCCTCTGCCATTTGAGAGACAACGATCACGCCAATTTCGCCCTTAAGGTCGTTGACAGAGACGCTTTAAGTAACAAGAAGCTTTTGCAGGTTCAAACGGAATCCGAGATTCTCGCTTTGCTCGACAACCCTTTTCTCCCTACTCTCTACGCGCGTCTCGACGTCTCTCACTACACGTGCCTCCTTATTGACTACTGCCCAGCCGGTGACCTCCACTCTCTCCTCCGCAAACAGCCTGGAAACCGCCTCTCCATTTCGGCGGCGAGATTCTTCGTCGCTGAGGTTCTGGTGGCGCTTGAGTATCTTCACGCGCTTGGGATTGTGTACCGGGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCACTGATTTCGATTTGTGTTTCAAGTCCGACGTTGTCCCCACTTTTCACACGTCGACACAGCGGGGACCACAGGGTACTGCCTCCTGCTTTGGGATGAGGACCGCGGCGGAGGAGATTGTCGGGGAGTTCGTGGCGGAGCCGACGACGGCCTTTTCGAAGTCATGTGTAGGGACTCATGAGTATTTGGCGCCTGAGTTGGTAACCGGCGGTGGCCACGGAAACGCCGTCGACTGGTGGGCGTTTGGGGTGTTCATTTACGAGCTGCTACACGGAACGACGCCGTTTAAGGGAGTGAACAAGGAGGGTACGCTTAGAAACATAGCGTCTACAGAAGGGGTGAAGTTCAGGGCGGCCATGGAGGAAGAGGAAGGTATAGCAGAGGCGAGGGATCTGATAGAGAGACTACTGGTGAAAGATCCGGCGAGACGGTTGGGATCCGGGAAAGGGGCGACGGAGATCAAACGGCATCCATTCTTCAACGGTATAAAATGGGCGTTAATCCGGATGTACCGACCGCCGGAGGTGTACGGATTGATGAGAAAAGCCAGATCACACGTGAGCCACGTGCGTGACGTCAACCACCGCATGCGGCGTCGGAGCTGGTGGAACTGGAGAAAGCTCAGTAACTATTTAATTAAAAACGGAAATTCCAGTAAATCTCATACCAATACCAATTCCAATACTAATAATTACAGATTTAACGCCAAAAATAAAATGGCTTAA

Protein sequence

MEDDSDLDFSFTSTISTSTARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWRKLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA
Homology
BLAST of Carg13109 vs. NCBI nr
Match: KAG6600472.1 (Serine/threonine-protein kinase WAG2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031118.1 Serine/threonine-protein kinase WAG2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTSTARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAATTLSS 60
           MEDDSDLDFSFTSTISTSTARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAATTLSS
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAATTLSS 60

Query: 61  DGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQTESEI 120
           DGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQTESEI
Sbjct: 61  DGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQTESEI 120

Query: 121 LALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLV 180
           LALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLV
Sbjct: 121 LALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLV 180

Query: 181 ALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTASCF 240
           ALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTASCF
Sbjct: 181 ALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTASCF 240

Query: 241 GMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELLH 300
           GMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELLH
Sbjct: 241 GMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELLH 300

Query: 301 GTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLGSGKGA 360
           GTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLGSGKGA
Sbjct: 301 GTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLGSGKGA 360

Query: 361 TEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWRKLSNY 420
           TEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWRKLSNY
Sbjct: 361 TEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWRKLSNY 420

Query: 421 LIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           LIKNGNSSKSHTNTNSNTNNYRFNAKNKMA
Sbjct: 421 LIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 450

BLAST of Carg13109 vs. NCBI nr
Match: XP_022942107.1 (serine/threonine-protein kinase WAG1-like [Cucurbita moschata])

HSP 1 Score: 874.4 bits (2258), Expect = 4.2e-250
Identity = 439/455 (96.48%), Postives = 442/455 (97.14%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRGFSALNLRPHRHSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLARSSLTLSFNESRGFSALNLRPHRHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
           TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS+SAARFFV
Sbjct: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFV 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240

Query: 241 TASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300
           T  CFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI
Sbjct: 241 TPCCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300

Query: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360
           YELLHGTTPF+GVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG
Sbjct: 301 YELLHGTTPFRGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360

Query: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420
           SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR
Sbjct: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420

Query: 421 KLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           KL NYLIKNGNSSKSHT    NTN+YRFNAKNKMA
Sbjct: 421 KLCNYLIKNGNSSKSHT----NTNHYRFNAKNKMA 451

BLAST of Carg13109 vs. NCBI nr
Match: XP_022980840.1 (serine/threonine-protein kinase WAG1-like [Cucurbita maxima])

HSP 1 Score: 863.6 bits (2230), Expect = 7.5e-247
Identity = 432/455 (94.95%), Postives = 438/455 (96.26%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRG SALNLRPH HSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLARSSLTLSFNESRGSSALNLRPHHHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDG+LHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGNLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
            ESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS+SAARFFV
Sbjct: 121 MESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFV 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240

Query: 241 TASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300
            A CFGM TAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPEL+TGGGHGNAVDWWAFGVFI
Sbjct: 241 AACCFGMGTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELLTGGGHGNAVDWWAFGVFI 300

Query: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360
           YELLHGTTPFKGVNKEGTLRNIASTEGVKF+AAMEEEEGI EARDL+ERLLVKDPARRLG
Sbjct: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFKAAMEEEEGIEEARDLVERLLVKDPARRLG 360

Query: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420
           SGKGATEIKRHPFFNGIKW LIRMYRPPEVYGLMRKARSHV HVRDVNHRMRRR WWNWR
Sbjct: 361 SGKGATEIKRHPFFNGIKWPLIRMYRPPEVYGLMRKARSHVRHVRDVNHRMRRRGWWNWR 420

Query: 421 KLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           KLSNYLIKNG SSKSHTNTNSNTN+YRFNAKNKMA
Sbjct: 421 KLSNYLIKNGISSKSHTNTNSNTNHYRFNAKNKMA 455

BLAST of Carg13109 vs. NCBI nr
Match: XP_023554515.1 (serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 853.2 bits (2203), Expect = 1.0e-243
Identity = 432/455 (94.95%), Postives = 436/455 (95.82%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRG S LNLRPHRHSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLARSSLTLSFNESRGSSTLNLRPHRHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
           TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS+SAARFFV
Sbjct: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFV 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQ GPQG
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQPGPQG 240

Query: 241 TASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300
           TASCFG RTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPEL+TGGGHGNAVDWWAFGVFI
Sbjct: 241 TASCFGRRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELITGGGHGNAVDWWAFGVFI 300

Query: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360
           YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG
Sbjct: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360

Query: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420
           SGKGATEIKRHPFF GIKWALIRMYRPPEVYGLMRKARSHV    DVNHR RRR WWNWR
Sbjct: 361 SGKGATEIKRHPFFKGIKWALIRMYRPPEVYGLMRKARSHVG---DVNHRTRRRGWWNWR 420

Query: 421 KLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           KLSNYLIKNGNS  S+TNTNSNTN+YRFNAKNKMA
Sbjct: 421 KLSNYLIKNGNS--SNTNTNSNTNHYRFNAKNKMA 450

BLAST of Carg13109 vs. NCBI nr
Match: XP_023522923.1 (serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 787.3 bits (2032), Expect = 6.8e-224
Identity = 398/443 (89.84%), Postives = 411/443 (92.78%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRG S LNLRPHRHSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSIARSSLTLSFNESRGSSTLNLRPHRHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
           TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP  R S++AARFF 
Sbjct: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPNYRFSVAAARFFA 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVML+DFDLCFKS+VVPTFHT TQ GPQ 
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSEVVPTFHTWTQPGPQS 240

Query: 241 TASCFGMRTAA---EEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFG 300
           T SCFG RTAA   +EIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN VDWWAFG
Sbjct: 241 T-SCFGWRTAAAESQEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNGVDWWAFG 300

Query: 301 VFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPAR 360
           VF+YELLHGTTPFKGVNKEGTLRNIAS+E VKFRAA+ EEEG+AEARDL+ERLLVKDPAR
Sbjct: 301 VFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFRAAV-EEEGMAEARDLVERLLVKDPAR 360

Query: 361 RLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWW 420
           RLGSGKGATEIKRHPFF+GIKW LIR YR PEVYGLMRKARSHVSHV  V+HRMRRR WW
Sbjct: 361 RLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKARSHVSHVTGVSHRMRRRGWW 420

Query: 421 NWRKLSNYLIKNGNSSKSHTNTN 436
           NWRKLSN L KNGN+SKSHTN N
Sbjct: 421 NWRKLSNGLFKNGNASKSHTNNN 441

BLAST of Carg13109 vs. ExPASy Swiss-Prot
Match: Q9C8M5 (Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=2 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 2.7e-154
Identity = 290/472 (61.44%), Postives = 344/472 (72.88%), Query Frame = 0

Query: 4   DSDLDFSFTSTIS----------TSTARSSLTLSFNE---------SRGFSALNLRPHRH 63
           D+DLD SFTST +          +S ARSSLTLSFN+         +   SA     HR 
Sbjct: 9   DTDLDLSFTSTATDRTFTSSSARSSLARSSLTLSFNDRLSTATTPSTTTSSAATTLHHRR 68

Query: 64  SDSHWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRD-NDHANFALKVVDR 123
            D HW++I+AATTLSSDG LHLRH KL++HLGTGNLGRVFLCHLRD  +   FALKV+DR
Sbjct: 69  YDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPNPTGFALKVIDR 128

Query: 124 DALSNKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPG 183
           D L+ KK+  V+TE+EIL+LLD+PFLPTLYAR+D SHYTCLLIDYCP GDLHSLLRKQP 
Sbjct: 129 DVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNGDLHSLLRKQPN 188

Query: 184 NRLSISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVP 243
           NRL IS  RFF AEVLVALEYLHALGIVYRDLKPEN+L+REDGH+ML+DFDLCFK+DVVP
Sbjct: 189 NRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSDFDLCFKADVVP 248

Query: 244 TFH-------TSTQRGPQGTASCFG--MRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAP 303
           TF        +S+ R  +    CF   +    EEIV EF AEP TAFSKSCVGTHEYLAP
Sbjct: 249 TFRSRRFRRTSSSPRKTRRGGGCFSTEVEYEREEIVAEFAAEPVTAFSKSCVGTHEYLAP 308

Query: 304 ELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEG 363
           ELV G GHG+ VDWWAFG+F+YE+L+GTTPFKG  KE TLRNI S + V F     EEEG
Sbjct: 309 ELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDVAFTL---EEEG 368

Query: 364 IAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARS 423
           + EA+DLIE+LLVKDP +RLG  +GA +IKRH FF GIKW LIR Y+PPE+ GL++K ++
Sbjct: 369 MVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPPEIRGLVKKTKA 428

Query: 424 HVSHVRDVNHRMRRRSWWNWRKLSNYLIKNGNSSKSHTNTNSNTNNYRFNAK 447
           H  HV        RR+ W W  LS +L+++ + SKS +   SN N Y +  K
Sbjct: 429 HAGHVTAA--VTPRRNKWLWWALS-HLLRSKSLSKSSSKIQSNNNYYHYVGK 474

BLAST of Carg13109 vs. ExPASy Swiss-Prot
Match: Q9LUL2 (Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 6.8e-150
Identity = 297/475 (62.53%), Postives = 351/475 (73.89%), Query Frame = 0

Query: 4   DSDLDFSFTS-----TISTSTARSSLTLSFNE-----------SRGFSALNLRPHRHSDS 63
           D+DLD SFTS     T ++S+AR+SLTLSFN+           S   S++N   HR  D 
Sbjct: 10  DTDLDLSFTSTTTDRTFASSSARTSLTLSFNDRLSTSSAVTTSSTSSSSVN---HRRHDP 69

Query: 64  HWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDAL- 123
           HWSAIK+A  LSSDG++HLRHLKLI+HLGTGNLGRVFLC+LRD+  A FALKV+DR+ L 
Sbjct: 70  HWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVIDRNCLT 129

Query: 124 SNKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRL 183
           + KKL QV+TE+EIL+LLD+PFLPTLYAR+D SHYTCLLIDY P GDLHSLLRKQPGNRL
Sbjct: 130 TEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRKQPGNRL 189

Query: 184 SISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFH 243
            I   RFF AEVLVALEYLHA+GIVYRDLKPENVLLREDGHVML+DFDLCFKSDVVPTF 
Sbjct: 190 PIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFK 249

Query: 244 TSTQRGPQGTAS-------CFGM----RTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPE 303
           +   R    + S       CF +    +   EEIV EF AEP TAFS+SCVGTHEYLAPE
Sbjct: 250 SRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTHEYLAPE 309

Query: 304 LVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGI 363
           LV+G GHG+ VDWWAFG+F+YELL+GTTPFKG +KE TLRNI ST      A+   +  +
Sbjct: 310 LVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNIVSTTKT---ASFHMDGDL 369

Query: 364 AEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPP-EVYGLM--RKA 423
            EARDLIE+LLVKDP +RLG  +GA +IKRHPFF+GIKW LIR Y+PP EV GL+  +  
Sbjct: 370 DEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVRGLVIKKST 429

Query: 424 RSHVSHVRDVNHRMRRRSWWNWRKLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKN 448
           R H SHV  V+   RRR  + WR LS YL++ G SS   +   SN+N Y +  K+
Sbjct: 430 RPHASHVIAVS--PRRRKSFLWRALS-YLLR-GKSSSGGSKNQSNSNYYHYVGKS 473

BLAST of Carg13109 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.7e-92
Identity = 189/409 (46.21%), Postives = 247/409 (60.39%), Query Frame = 0

Query: 17  TSTARSSLTLSFNESRGFSAL--NLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIKH 76
           ++T R S +   ++    S+   N +PH+ +D  W AI+A  T    G L L H +L+K 
Sbjct: 71  SNTCRPSTSSDISDESTCSSFSGNNKPHKANDVRWEAIQAVRT--KHGVLGLNHFRLLKR 130

Query: 77  LGTGNLGRVFLCHLRDNDHANFALKVVDRDAL-SNKKLLQVQTESEILALLDNPFLPTLY 136
           LG G++G V L  L       FA+KV+D+ AL S KKLL+ QTE EIL  LD+PFLPTLY
Sbjct: 131 LGCGDIGTVHLAELH-GTRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLY 190

Query: 137 ARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALGIVYR 196
           +  +   ++CL++++CP GDLH+L ++QPG R S  AA+F+VAEVL+A+EYLH LGI+YR
Sbjct: 191 SHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYR 250

Query: 197 DLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG------------------ 256
           DLKPENVL+R+DGHVML+DFDL  +  V PT   ST    +G                  
Sbjct: 251 DLKPENVLVRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSC 310

Query: 257 ---TASCFGMRTAAEEI----------------VGEFVAEPTTAFSKSCVGTHEYLAPEL 316
                +CF  R  + +                 + E VAEPT+A S S VGTHEYLAPE+
Sbjct: 311 ISAPTTCFSPRYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEI 370

Query: 317 VTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIA 376
           + G GHG+AVDWW FG+F+YELL G TPFKG     TL N+    G   R         A
Sbjct: 371 IKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV---GQPLRFPESPVVSFA 430

Query: 377 EARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEV 386
            ARDLI  LLVK+P  RL   +GATE+K+HPFF G+ WAL+R   PPE+
Sbjct: 431 -ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEI 472

BLAST of Carg13109 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.9e-92
Identity = 190/414 (45.89%), Postives = 248/414 (59.90%), Query Frame = 0

Query: 17  TSTARSSLTLSFNES---RGFSALNLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIK 76
           +ST R S +   ++      FS+   +PH+ +D  W AI+A  T    G L L H +L+K
Sbjct: 56  SSTCRPSTSSDISDESTCSSFSSSINKPHKANDVRWEAIQAVRT--KHGGLGLNHFRLLK 115

Query: 77  HLGTGNLGRVFLCHLRDNDHANFALKVVDRDAL-SNKKLLQVQTESEILALLDNPFLPTL 136
            LG G++G V L  L +     FA+KV+D+ AL S KKLL+ QTE EIL  LD+PFLPTL
Sbjct: 116 RLGCGDIGTVHLAEL-NGTRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTL 175

Query: 137 YARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALGIVY 196
           Y+  +   ++CL++++CP GDLH+L ++QPG R +  AA+F+VAEVL+A+EYLH LGI+Y
Sbjct: 176 YSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIY 235

Query: 197 RDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTA--------------- 256
           RDLKPENVL+R+DGHVML+DFDL  +  V  +   S   G +G +               
Sbjct: 236 RDLKPENVLVRDDGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQ 295

Query: 257 ---------SCFGMRTAAEEI-----------------VGEFVAEPTTAFSKSCVGTHEY 316
                    SCFG R  + +                  + E VAEPT A S S VGTHEY
Sbjct: 296 PSCISMAPTSCFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEY 355

Query: 317 LAPELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEE 376
           LAPE++ G GHG+AVDWW FG+F+YELL G TPFKG     TL N+    G   R     
Sbjct: 356 LAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV---GQPLRFPESP 415

Query: 377 EEGIAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEV 386
               A ARDLI  LLVK+P  RL   +GATEIK+HPFF G+ WAL+R   PPE+
Sbjct: 416 VVSFA-ARDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEI 462

BLAST of Carg13109 vs. ExPASy Swiss-Prot
Match: Q2QM77 (Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 8.4e-92
Identity = 188/388 (48.45%), Postives = 244/388 (62.89%), Query Frame = 0

Query: 16  STSTARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIKHL 75
           S S A ++   S +  R  +++ LRPHR  D  W+AI+AA+T S+   L  R  KL++ +
Sbjct: 61  SPSAAVAAAATSCSPPR--ASVLLRPHRSGDVAWAAIRAASTTSA-APLGPRDFKLVRRI 120

Query: 76  GTGNLGRVFLCHLRDNDHAN----FALKVVDRDALSNK-KLLQVQTESEILALLDNPFLP 135
           G G++G V+LC LR +        +A+KVVDR A++ K KL +   E  IL  LD+PFLP
Sbjct: 121 GGGDIGTVYLCRLRSSPERESPCMYAMKVVDRRAVARKQKLGRAAAEKRILRQLDHPFLP 180

Query: 136 TLYARLDVS-HYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALG 195
           TL+A  D + H++C ++++CP GDLHSL  + P  R  + +ARF+ AEVL+A+EYLH +G
Sbjct: 181 TLFADFDATPHFSCAVMEFCPGGDLHSLRHRMPSRRFPLPSARFYAAEVLLAIEYLHMMG 240

Query: 196 IVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTF--HTSTQRGPQGTASCF------- 255
           IVYRDLKPENVL+R DGH+MLTDFDL  +S   P+    T T     G ASCF       
Sbjct: 241 IVYRDLKPENVLIRADGHIMLTDFDLSLQSTTSPSLDGDTDTDDEASGGASCFPDHLLRF 300

Query: 256 -GMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELL 315
              R A       FVAEP  A S S VGTHEY+APE+ +GG HG AVDWWA+GVF+YEL+
Sbjct: 301 KRRRNAVAAPRPRFVAEPVDARSCSFVGTHEYVAPEVASGGAHGAAVDWWAYGVFLYELI 360

Query: 316 HGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLGSGKG 375
           +G TPF G   E TLRNI          +       A+ARDLI RLL KDPA RLGS +G
Sbjct: 361 YGRTPFAGATNEATLRNIVRRPLAFPSGSGSCGPADADARDLIARLLAKDPAARLGSRRG 420

Query: 376 ATEIKRHPFFNGIKWALIRMYRPPEVYG 388
           A ++K HPFF  +  AL+R  RPP V G
Sbjct: 421 AADVKSHPFFKSLNLALLRSSRPPVVPG 445

BLAST of Carg13109 vs. ExPASy TrEMBL
Match: A0A6J1FPC0 (serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC111447279 PE=4 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.1e-250
Identity = 439/455 (96.48%), Postives = 442/455 (97.14%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRGFSALNLRPHRHSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLARSSLTLSFNESRGFSALNLRPHRHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
           TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS+SAARFFV
Sbjct: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFV 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240

Query: 241 TASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300
           T  CFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI
Sbjct: 241 TPCCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300

Query: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360
           YELLHGTTPF+GVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG
Sbjct: 301 YELLHGTTPFRGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360

Query: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420
           SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR
Sbjct: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420

Query: 421 KLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           KL NYLIKNGNSSKSHT    NTN+YRFNAKNKMA
Sbjct: 421 KLCNYLIKNGNSSKSHT----NTNHYRFNAKNKMA 451

BLAST of Carg13109 vs. ExPASy TrEMBL
Match: A0A6J1IXP2 (serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC111480110 PE=4 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 3.6e-247
Identity = 432/455 (94.95%), Postives = 438/455 (96.26%), Query Frame = 0

Query: 1   MEDDSDLDFSFTSTISTST-----ARSSLTLSFNESRGFSALNLRPHRHSDSHWSAIKAA 60
           MEDDSDLDFSFTSTISTST     ARSSLTLSFNESRG SALNLRPH HSD HWSAIKAA
Sbjct: 1   MEDDSDLDFSFTSTISTSTARSSLARSSLTLSFNESRGSSALNLRPHHHSDPHWSAIKAA 60

Query: 61  TTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120
           TTLSSDG+LHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ
Sbjct: 61  TTLSSDGNLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNKKLLQVQ 120

Query: 121 TESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFV 180
            ESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS+SAARFFV
Sbjct: 121 MESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVSAARFFV 180

Query: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240
           AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG
Sbjct: 181 AEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG 240

Query: 241 TASCFGMRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAVDWWAFGVFI 300
            A CFGM TAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPEL+TGGGHGNAVDWWAFGVFI
Sbjct: 241 AACCFGMGTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPELLTGGGHGNAVDWWAFGVFI 300

Query: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLLVKDPARRLG 360
           YELLHGTTPFKGVNKEGTLRNIASTEGVKF+AAMEEEEGI EARDL+ERLLVKDPARRLG
Sbjct: 301 YELLHGTTPFKGVNKEGTLRNIASTEGVKFKAAMEEEEGIEEARDLVERLLVKDPARRLG 360

Query: 361 SGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRMRRRSWWNWR 420
           SGKGATEIKRHPFFNGIKW LIRMYRPPEVYGLMRKARSHV HVRDVNHRMRRR WWNWR
Sbjct: 361 SGKGATEIKRHPFFNGIKWPLIRMYRPPEVYGLMRKARSHVRHVRDVNHRMRRRGWWNWR 420

Query: 421 KLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNKMA 451
           KLSNYLIKNG SSKSHTNTNSNTN+YRFNAKNKMA
Sbjct: 421 KLSNYLIKNGISSKSHTNTNSNTNHYRFNAKNKMA 455

BLAST of Carg13109 vs. ExPASy TrEMBL
Match: A0A6J1J9F8 (serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC111482931 PE=4 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.2e-211
Identity = 379/451 (84.04%), Postives = 402/451 (89.14%), Query Frame = 0

Query: 4   DSDLDFSFTSTIS----------TSTARSSLTLSFNESR------GFSALNLRPHRHSDS 63
           DSDLDFSFTST +          TS ARSSLTLSFNESR        +A++ RPH HSD 
Sbjct: 11  DSDLDFSFTSTTTDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAAAVHHRPHHHSDP 70

Query: 64  HWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALS 123
           HWSAIKAAT LSSDGHLHLRHLKLI+HLGTGNLGRVFLCHLRDNDHA+FALKVVD+DALS
Sbjct: 71  HWSAIKAATALSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDKDALS 130

Query: 124 NKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS 183
           NKKLLQVQTESEILA L++PFLPTLYARLDVSHYTCLLIDYCP+GDLHSLLRKQP  R S
Sbjct: 131 NKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPSGDLHSLLRKQPNYRFS 190

Query: 184 ISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHT 243
           ++AARFF AEVLVALEYLHALGIVYRDLKPENVLLREDGHVML+DFDLCFKS+VVPTFHT
Sbjct: 191 VAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSEVVPTFHT 250

Query: 244 STQRGPQGTASCFGMRTAA---EEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN 303
            TQ GPQ T SCFG RTAA   +EIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN
Sbjct: 251 WTQPGPQST-SCFGWRTAAVESQEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN 310

Query: 304 AVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIER 363
            VDWWAFGVF+YELLHGTTPFKGVNKEGTLRNIAS+EGVKFRAA+ EEEG+AEARDLIER
Sbjct: 311 GVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRAAV-EEEGMAEARDLIER 370

Query: 364 LLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNH 423
           LLVKDPARRLGSGKGATEIKRHPFF+GIKW LIR YR PEVYGLMRKARSHVSHV   +H
Sbjct: 371 LLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKARSHVSHVTGASH 430

Query: 424 RMRRRSWWNWRKLSNYLIKNGNSSKSHTNTN 436
           RMRRR WWNWRKLSN L KNG++SKSHTN N
Sbjct: 431 RMRRRGWWNWRKLSNGLFKNGSASKSHTNNN 459

BLAST of Carg13109 vs. ExPASy TrEMBL
Match: A0A6J1ETX0 (serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC111437682 PE=4 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 1.0e-209
Identity = 378/451 (83.81%), Postives = 401/451 (88.91%), Query Frame = 0

Query: 4   DSDLDFSFTSTIS----------TSTARSSLTLSFNESR------GFSALNLRPHRHSDS 63
           DSDLDFSFTST +          TS ARSSLTLSFNESR        +A+N RPH HSD 
Sbjct: 11  DSDLDFSFTSTTTDRTFASSSARTSLARSSLTLSFNESRLAAAAAAAAAVNHRPHHHSDP 70

Query: 64  HWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALS 123
           HWSAIKAAT LSSDG LHLR+LKL++HLGTGNLGRVFLCHLRDNDHA+FALKVVD+DALS
Sbjct: 71  HWSAIKAATALSSDGRLHLRNLKLVRHLGTGNLGRVFLCHLRDNDHASFALKVVDKDALS 130

Query: 124 NKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS 183
           NKKLLQVQTESEILA L++PFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP  R S
Sbjct: 131 NKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPSYRFS 190

Query: 184 ISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHT 243
           ++AARFF AEVLVALEYLHALGIVYRDLKPENVLLREDGHVML+DFDLCFKS+VVPTFHT
Sbjct: 191 VAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSEVVPTFHT 250

Query: 244 STQRGPQGTASCFGMRTAA---EEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN 303
            TQ GPQ T SCFG RTAA   +EIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN
Sbjct: 251 WTQPGPQST-SCFGWRTAAAEPQEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGN 310

Query: 304 AVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIER 363
            VDWWAFGVF+YELLHGTTPFKGVNKEGTLRNIAS+E VKFRAA+ EEEG+AEARDLIER
Sbjct: 311 GVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFRAAV-EEEGMAEARDLIER 370

Query: 364 LLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNH 423
           LLVKDPARRLGSGKGATEIKRH FF+GIKW LIR YR PEVYGLMRKARSHVSHV  V+H
Sbjct: 371 LLVKDPARRLGSGKGATEIKRHTFFDGIKWPLIRTYRAPEVYGLMRKARSHVSHVTGVSH 430

Query: 424 RMRRRSWWNWRKLSNYLIKNGNSSKSHTNTN 436
           RMRRR+WWNWRKLSN L KNGN+SKSHTN N
Sbjct: 431 RMRRRTWWNWRKLSNGLFKNGNASKSHTNNN 459

BLAST of Carg13109 vs. ExPASy TrEMBL
Match: A0A5D3D1P2 (Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001730 PE=4 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.8e-207
Identity = 375/463 (80.99%), Postives = 402/463 (86.83%), Query Frame = 0

Query: 4   DSDLDFSFTSTIS-----------TSTARSSLTLSFNESR---GFSALNLRPHRHSDSHW 63
           DSDLDFSFTST +           TS ARSSLTLSFNESR     +ALNLRPH  SDSHW
Sbjct: 11  DSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAALNLRPHHRSDSHW 70

Query: 64  SAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSNK 123
           SAI+AATTLSSDGHLHLRHLKLI+HLGTGNLGRVFLCHLRDNDHA+FALKVVD++ALSNK
Sbjct: 71  SAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDKEALSNK 130

Query: 124 KLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSIS 183
           KL QVQTE+EILA LD+PFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPG+R S++
Sbjct: 131 KLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGSRFSVA 190

Query: 184 AARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTST 243
           AARFF AEVLVALEYLHALGIVYRDLKPENVLLREDGHVML+DFDLCFKSDVVPTFHT T
Sbjct: 191 AARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFHTWT 250

Query: 244 QRGPQGTASCFGMRTA---AEEIVGEFVAEPTTAFSKSCVGTHEYLAPELVTGGGHGNAV 303
           + GP+ T  C G RT     EEIVGEFVAEPT AFSKSCVGTHEYLAPELV+GGGHGN V
Sbjct: 251 RPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLAPELVSGGGHGNGV 310

Query: 304 DWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIAEARDLIERLL 363
           DWWAFGVF+YELLHGTTPFKGVNKEGTLRNIAS+EGVKFRAA EEEEG+ EARDLIERLL
Sbjct: 311 DWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRAAGEEEEGMGEARDLIERLL 370

Query: 364 VKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARSHVSHVRDVNHRM 423
           VKDPA RLGS KGATEIKRHPFF GIKW LIR YR PEVYG+MRK RS VSHVR V+ R+
Sbjct: 371 VKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGRSQVSHVRGVSQRI 430

Query: 424 RRRSWWNWRK-LSNYLIKNGNSSKSHTNTNSNTNNYRFNAKNK 449
           RRR WWNWRK +SN LIKNG+ SKSH    SNTN Y +++K K
Sbjct: 431 RRRGWWNWRKVISNGLIKNGSCSKSH----SNTNFYSYSSKKK 469

BLAST of Carg13109 vs. TAIR 10
Match: AT1G53700.1 (WAG 1 )

HSP 1 Score: 546.6 bits (1407), Expect = 1.9e-155
Identity = 290/472 (61.44%), Postives = 344/472 (72.88%), Query Frame = 0

Query: 4   DSDLDFSFTSTIS----------TSTARSSLTLSFNE---------SRGFSALNLRPHRH 63
           D+DLD SFTST +          +S ARSSLTLSFN+         +   SA     HR 
Sbjct: 9   DTDLDLSFTSTATDRTFTSSSARSSLARSSLTLSFNDRLSTATTPSTTTSSAATTLHHRR 68

Query: 64  SDSHWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRD-NDHANFALKVVDR 123
            D HW++I+AATTLSSDG LHLRH KL++HLGTGNLGRVFLCHLRD  +   FALKV+DR
Sbjct: 69  YDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPNPTGFALKVIDR 128

Query: 124 DALSNKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPG 183
           D L+ KK+  V+TE+EIL+LLD+PFLPTLYAR+D SHYTCLLIDYCP GDLHSLLRKQP 
Sbjct: 129 DVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNGDLHSLLRKQPN 188

Query: 184 NRLSISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVP 243
           NRL IS  RFF AEVLVALEYLHALGIVYRDLKPEN+L+REDGH+ML+DFDLCFK+DVVP
Sbjct: 189 NRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSDFDLCFKADVVP 248

Query: 244 TFH-------TSTQRGPQGTASCFG--MRTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAP 303
           TF        +S+ R  +    CF   +    EEIV EF AEP TAFSKSCVGTHEYLAP
Sbjct: 249 TFRSRRFRRTSSSPRKTRRGGGCFSTEVEYEREEIVAEFAAEPVTAFSKSCVGTHEYLAP 308

Query: 304 ELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEG 363
           ELV G GHG+ VDWWAFG+F+YE+L+GTTPFKG  KE TLRNI S + V F     EEEG
Sbjct: 309 ELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDVAFTL---EEEG 368

Query: 364 IAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEVYGLMRKARS 423
           + EA+DLIE+LLVKDP +RLG  +GA +IKRH FF GIKW LIR Y+PPE+ GL++K ++
Sbjct: 369 MVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPPEIRGLVKKTKA 428

Query: 424 HVSHVRDVNHRMRRRSWWNWRKLSNYLIKNGNSSKSHTNTNSNTNNYRFNAK 447
           H  HV        RR+ W W  LS +L+++ + SKS +   SN N Y +  K
Sbjct: 429 HAGHVTAA--VTPRRNKWLWWALS-HLLRSKSLSKSSSKIQSNNNYYHYVGK 474

BLAST of Carg13109 vs. TAIR 10
Match: AT3G14370.1 (Protein kinase superfamily protein )

HSP 1 Score: 531.9 bits (1369), Expect = 4.8e-151
Identity = 297/475 (62.53%), Postives = 351/475 (73.89%), Query Frame = 0

Query: 4   DSDLDFSFTS-----TISTSTARSSLTLSFNE-----------SRGFSALNLRPHRHSDS 63
           D+DLD SFTS     T ++S+AR+SLTLSFN+           S   S++N   HR  D 
Sbjct: 10  DTDLDLSFTSTTTDRTFASSSARTSLTLSFNDRLSTSSAVTTSSTSSSSVN---HRRHDP 69

Query: 64  HWSAIKAATTLSSDGHLHLRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDAL- 123
           HWSAIK+A  LSSDG++HLRHLKLI+HLGTGNLGRVFLC+LRD+  A FALKV+DR+ L 
Sbjct: 70  HWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVIDRNCLT 129

Query: 124 SNKKLLQVQTESEILALLDNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRL 183
           + KKL QV+TE+EIL+LLD+PFLPTLYAR+D SHYTCLLIDY P GDLHSLLRKQPGNRL
Sbjct: 130 TEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRKQPGNRL 189

Query: 184 SISAARFFVAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFH 243
            I   RFF AEVLVALEYLHA+GIVYRDLKPENVLLREDGHVML+DFDLCFKSDVVPTF 
Sbjct: 190 PIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVVPTFK 249

Query: 244 TSTQRGPQGTAS-------CFGM----RTAAEEIVGEFVAEPTTAFSKSCVGTHEYLAPE 303
           +   R    + S       CF +    +   EEIV EF AEP TAFS+SCVGTHEYLAPE
Sbjct: 250 SRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTHEYLAPE 309

Query: 304 LVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGI 363
           LV+G GHG+ VDWWAFG+F+YELL+GTTPFKG +KE TLRNI ST      A+   +  +
Sbjct: 310 LVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNIVSTTKT---ASFHMDGDL 369

Query: 364 AEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPP-EVYGLM--RKA 423
            EARDLIE+LLVKDP +RLG  +GA +IKRHPFF+GIKW LIR Y+PP EV GL+  +  
Sbjct: 370 DEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVRGLVIKKST 429

Query: 424 RSHVSHVRDVNHRMRRRSWWNWRKLSNYLIKNGNSSKSHTNTNSNTNNYRFNAKN 448
           R H SHV  V+   RRR  + WR LS YL++ G SS   +   SN+N Y +  K+
Sbjct: 430 RPHASHVIAVS--PRRRKSFLWRALS-YLLR-GKSSSGGSKNQSNSNYYHYVGKS 473

BLAST of Carg13109 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 189/409 (46.21%), Postives = 247/409 (60.39%), Query Frame = 0

Query: 17  TSTARSSLTLSFNESRGFSAL--NLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIKH 76
           ++T R S +   ++    S+   N +PH+ +D  W AI+A  T    G L L H +L+K 
Sbjct: 71  SNTCRPSTSSDISDESTCSSFSGNNKPHKANDVRWEAIQAVRT--KHGVLGLNHFRLLKR 130

Query: 77  LGTGNLGRVFLCHLRDNDHANFALKVVDRDAL-SNKKLLQVQTESEILALLDNPFLPTLY 136
           LG G++G V L  L       FA+KV+D+ AL S KKLL+ QTE EIL  LD+PFLPTLY
Sbjct: 131 LGCGDIGTVHLAELH-GTRCFFAMKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLY 190

Query: 137 ARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALGIVYR 196
           +  +   ++CL++++CP GDLH+L ++QPG R S  AA+F+VAEVL+A+EYLH LGI+YR
Sbjct: 191 SHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYR 250

Query: 197 DLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQG------------------ 256
           DLKPENVL+R+DGHVML+DFDL  +  V PT   ST    +G                  
Sbjct: 251 DLKPENVLVRDDGHVMLSDFDLSLRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSC 310

Query: 257 ---TASCFGMRTAAEEI----------------VGEFVAEPTTAFSKSCVGTHEYLAPEL 316
                +CF  R  + +                 + E VAEPT+A S S VGTHEYLAPE+
Sbjct: 311 ISAPTTCFSPRYFSSKSKKDKKMKNETGNQVSPLPELVAEPTSARSMSFVGTHEYLAPEI 370

Query: 317 VTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEEEEGIA 376
           + G GHG+AVDWW FG+F+YELL G TPFKG     TL N+    G   R         A
Sbjct: 371 IKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV---GQPLRFPESPVVSFA 430

Query: 377 EARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEV 386
            ARDLI  LLVK+P  RL   +GATE+K+HPFF G+ WAL+R   PPE+
Sbjct: 431 -ARDLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEI 472

BLAST of Carg13109 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 339.7 bits (870), Expect = 3.5e-93
Identity = 190/414 (45.89%), Postives = 248/414 (59.90%), Query Frame = 0

Query: 17  TSTARSSLTLSFNES---RGFSALNLRPHRHSDSHWSAIKAATTLSSDGHLHLRHLKLIK 76
           +ST R S +   ++      FS+   +PH+ +D  W AI+A  T    G L L H +L+K
Sbjct: 56  SSTCRPSTSSDISDESTCSSFSSSINKPHKANDVRWEAIQAVRT--KHGGLGLNHFRLLK 115

Query: 77  HLGTGNLGRVFLCHLRDNDHANFALKVVDRDAL-SNKKLLQVQTESEILALLDNPFLPTL 136
            LG G++G V L  L +     FA+KV+D+ AL S KKLL+ QTE EIL  LD+PFLPTL
Sbjct: 116 RLGCGDIGTVHLAEL-NGTRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTL 175

Query: 137 YARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEYLHALGIVY 196
           Y+  +   ++CL++++CP GDLH+L ++QPG R +  AA+F+VAEVL+A+EYLH LGI+Y
Sbjct: 176 YSHFETEKFSCLVMEFCPGGDLHTLRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIY 235

Query: 197 RDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTA--------------- 256
           RDLKPENVL+R+DGHVML+DFDL  +  V  +   S   G +G +               
Sbjct: 236 RDLKPENVLVRDDGHVMLSDFDLSLRCTVSLSIVRSANVGSEGLSKNSVSCSQQPACIQQ 295

Query: 257 ---------SCFGMRTAAEEI-----------------VGEFVAEPTTAFSKSCVGTHEY 316
                    SCFG R  + +                  + E VAEPT A S S VGTHEY
Sbjct: 296 PSCISMAPTSCFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEY 355

Query: 317 LAPELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKFRAAMEE 376
           LAPE++ G GHG+AVDWW FG+F+YELL G TPFKG     TL N+    G   R     
Sbjct: 356 LAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVV---GQPLRFPESP 415

Query: 377 EEGIAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEV 386
               A ARDLI  LLVK+P  RL   +GATEIK+HPFF G+ WAL+R   PPE+
Sbjct: 416 VVSFA-ARDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEI 462

BLAST of Carg13109 vs. TAIR 10
Match: AT2G44830.1 (Protein kinase superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 6.0e-93
Identity = 197/420 (46.90%), Postives = 256/420 (60.95%), Query Frame = 0

Query: 10  SFTSTISTSTARSSLTLSFNESRGFS----ALNLRPHRHSDSHWSAIKAATTLSSDGHLH 69
           SF ST  TS +R+S +   +E   +S    +LN +PH+ +D  W+AI A  T   DG L 
Sbjct: 304 SFEST-KTSASRASDSSGLSEESSWSNFTGSLN-KPHKGNDPWWNAILAIRT--RDGILG 363

Query: 70  LRHLKLIKHLGTGNLGRVFLCHLRDNDHANFALKVVDRDALSN-KKLLQVQTESEILALL 129
           + H KL+K LG G++G V+L  L      +FA+KV+D+ +L + KKL + QTE +IL LL
Sbjct: 364 MSHFKLLKRLGCGDIGSVYLAEL-SGTRCHFAVKVMDKASLEDRKKLNRAQTERDILQLL 423

Query: 130 DNPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSISAARFFVAEVLVALEY 189
           D+PFLPTLY   +   ++CL+++YCP GDLH+L ++QPG   S  AARF+ AEVL+ALEY
Sbjct: 424 DHPFLPTLYTHFETDRFSCLVMEYCPGGDLHTLRQRQPGKHFSEYAARFYAAEVLLALEY 483

Query: 190 LHALGIVYRDLKPENVLLREDGHVMLTDFDLCFKSDVVPTFHTSTQRGPQGTA------- 249
           LH LG+VYRDLKPENVL+R+DGH+ML+DFDL  +  V PT   +    P           
Sbjct: 484 LHMLGVVYRDLKPENVLVRDDGHIMLSDFDLSLRCAVSPTLIKTFDSDPSRRGAFCVQPA 543

Query: 250 ------------SCF--------------GMRTAAEEI------VGEFVAEPTTAFSKSC 309
                       SCF                +T A+        + E VAEP T  S S 
Sbjct: 544 CMEPTSACIIQPSCFLPRSIFPNKNKKNKSRKTQADFFKSHSGSLPELVAEPNTR-SMSF 603

Query: 310 VGTHEYLAPELVTGGGHGNAVDWWAFGVFIYELLHGTTPFKGVNKEGTLRNIASTEGVKF 369
           VGTHEYLAPE++ G GHG+AVDWW FG+F++ELL+G TPFKG     TL N+   E +KF
Sbjct: 604 VGTHEYLAPEIIKGEGHGSAVDWWTFGIFVHELLYGKTPFKGSGNRATLFNVVG-EQLKF 663

Query: 370 RAAMEEEEGIAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFNGIKWALIRMYRPPEV 386
               E        RDLI+ LLVKDP  RLG+ +GATEIK+HPFF G+ WALIR   PPEV
Sbjct: 664 ---PESPATSYAGRDLIQALLVKDPKNRLGTKRGATEIKQHPFFEGVNWALIRCSTPPEV 713

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600472.15.0e-259100.00Serine/threonine-protein kinase WAG2, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022942107.14.2e-25096.48serine/threonine-protein kinase WAG1-like [Cucurbita moschata][more]
XP_022980840.17.5e-24794.95serine/threonine-protein kinase WAG1-like [Cucurbita maxima][more]
XP_023554515.11.0e-24394.95serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo][more]
XP_023522923.16.8e-22489.84serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C8M52.7e-15461.44Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=... [more]
Q9LUL26.8e-15062.53Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=... [more]
Q9SUA31.7e-9246.21Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1... [more]
Q9FG744.9e-9245.89Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=... [more]
Q2QM778.4e-9248.45Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FPC02.1e-25096.48serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IXP23.6e-24794.95serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1J9F84.2e-21184.04serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1ETX01.0e-20983.81serine/threonine-protein kinase WAG1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3D1P24.8e-20780.99Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G53700.11.9e-15561.44WAG 1 [more]
AT3G14370.14.8e-15162.53Protein kinase superfamily protein [more]
AT4G26610.11.2e-9346.21D6 protein kinase like 1 [more]
AT5G55910.13.5e-9345.89D6 protein kinase [more]
AT2G44830.16.0e-9346.90Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 69..369
e-value: 8.3E-76
score: 267.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 71..369
e-value: 3.3E-52
score: 177.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 69..369
score: 41.770931
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 153..383
e-value: 5.2E-77
score: 260.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 68..152
e-value: 5.2E-77
score: 260.6
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 73..215
e-value: 4.3E-15
score: 52.9
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 264..379
e-value: 1.2E-26
score: 91.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 425..450
NoneNo IPR availablePANTHERPTHR45637:SF2SERINE/THREONINE-PROTEIN KINASE WAG1coord: 3..402
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 3..402
NoneNo IPR availableCDDcd05574STKc_phototropin_likecoord: 67..385
e-value: 6.58329E-164
score: 462.477
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 190..202
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 45..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13109-RACarg13109-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity