Carg12968 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12968
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncytochrome P450 CYP72A219-like
LocationCarg_Chr10: 3500802 .. 3502192 (+)
RNA-Seq ExpressionCarg12968
SyntenyCarg12968
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTACTTTGTGTTCTATTCATTGGCACTTTGCTTGGTTGTTTTTGGAGTGGCAAAAGCTTTCTACTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGACAAGGAGTTAGGGGCAGTTCCTACAAGCCTTTGGTTGGTGATGCCAAAGAGTATGTGAGCATGATCACTGAAGCGTGGTCGACTCCCATGAATCTAGACCATCAGATCATTCAACGTGTCGATCCGTTCACTGCGGTGTATGCTAAAAAATATGGTATGTTGTACTGTGTCGTGTCGTGTCTAGTATTAGAATGAATCTGTCGATCCATTCACGTCCATGTATACTAAAAAGTATGGTATGTTGTAACGTGTCGTGTCGTGTATTAGAATGAATCTGTCGATCCATTCACGTCAGTGTATGCTAAAAAGTATGGTATGTTGTGTCGTGTCTAGTATTAGAATGAATTTGTGGATCCGTTCACGACAGTGTATGCTAAAAAGTATGGTATGTTGTACTGAGTTGTGTCGTGTCTAGTATTGAATGAATCTATCGATCCATTCACATCAGTGTATGCTAAAAAATATGGTATCTTGTACCATGTCATGTTGTGCTATGTCTAGTATTAGAATGAATCTCCCATCCATTCATGTCAGTGTATGCTAAAACATATGGTATGTTGTACCGTGTCGTGTTGTGTCTAGTATTAGAATGGATCTTAATTCTTCGTCATTTATGTGTAGGAAAGTTATCGATGTGCTGGTTTGGGACAAGTCCGAGATTGATGGTGAAGGACCCCGAGATGATGAAAGAAGTACTATCAAACAAGGAGGGTAGCTTTGAGAAGCCACCGATGAACCCACTTATTCTGAAGCTGACCAAGGGATTAACTACTCTTAAAGGGGAGGAATGGGCTAAACACAGGAGGATTATCAATCCTGCTTTTCATCTGGAAAGGCTCAAGGTACATACACAGTTTAAGAATTCATTGGCTAAGTTCTAAACTAGAGTGGTGGAAGTTAAGAACATAATGATAATGTTTGGTTGATGAAGGGAATGATGCCAGCCTTCACCATAAGCTGCAGCAGCATGATAGAGCGCTGGAAGGAGATGGCTGGAAATCAAGAGAGTGTTGAAGTGGATGTGTGGCCTGAAATTCAGAGACTAACAGCAGATGTTATATCCAGGGCAGCCTTTGGCTCCAACTTTGAAGAAGGGAAGAAACTTTTTGAACTTCTCAAACAACTCACTCTTTTTACTGTTGAAGCCATGCAGACCTTGTATCTTCCTGGTTTCAGGTTAAATTTTGCTTCCCCTTACCACCTATAATTTTCCTGTCCTTCGAATATCTGGGGTGAGAATGATCAGTAAATGAACATAATTGTGAGTGCTTACAAGT

mRNA sequence

ATGGAGTACTTTGTGTTCTATTCATTGGCACTTTGCTTGGTTGTTTTTGGAGTGGCAAAAGCTTTCTACTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGACAAGGAGTTAGGGGCAGTTCCTACAAGCCTTTGGTTGGTGATGCCAAAGAGTATGTGAGCATGATCACTGAAGCGTGGTCGACTCCCATGAATCTAGACCATCAGATCATTCAACGTGTCGATCCGTTCACTGCGGTGTATGCTAAAAAATATGGAAAGTTATCGATGTGCTGGTTTGGGACAAGTCCGAGATTGATGGGAATGATGCCAGCCTTCACCATAAGCTGCAGCAGCATGATAGAGCGCTGGAAGGAGATGGCTGGAAATCAAGAGAGTGTTGAAGTGGATGTGTGGCCTGAAATTCAGAGACTAACAGCAGATGTTATATCCAGGGCAGCCTTTGGCTCCAACTTTGAAGAAGGGAAGAAACTTTTTGAACTTCTCAAACAACTCACTCTTTTTACTGTTGAAGCCATGCAGACCTTGTATCTTCCTGGTTTCAGGTTAAATTTTGCTTCCCCTTACCACCTATAATTTTCCTGTCCTTCGAATATCTGGGGTGAGAATGATCAGTAAATGAACATAATTGTGAGTGCTTACAAGT

Coding sequence (CDS)

ATGGAGTACTTTGTGTTCTATTCATTGGCACTTTGCTTGGTTGTTTTTGGAGTGGCAAAAGCTTTCTACTCCATTTGGTGGAGACCCAAATTGGTGGAGAGACAGTTGAAAAGACAAGGAGTTAGGGGCAGTTCCTACAAGCCTTTGGTTGGTGATGCCAAAGAGTATGTGAGCATGATCACTGAAGCGTGGTCGACTCCCATGAATCTAGACCATCAGATCATTCAACGTGTCGATCCGTTCACTGCGGTGTATGCTAAAAAATATGGAAAGTTATCGATGTGCTGGTTTGGGACAAGTCCGAGATTGATGGGAATGATGCCAGCCTTCACCATAAGCTGCAGCAGCATGATAGAGCGCTGGAAGGAGATGGCTGGAAATCAAGAGAGTGTTGAAGTGGATGTGTGGCCTGAAATTCAGAGACTAACAGCAGATGTTATATCCAGGGCAGCCTTTGGCTCCAACTTTGAAGAAGGGAAGAAACTTTTTGAACTTCTCAAACAACTCACTCTTTTTACTGTTGAAGCCATGCAGACCTTGTATCTTCCTGGTTTCAGGTTAAATTTTGCTTCCCCTTACCACCTATAA

Protein sequence

MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMGMMPAFTISCSSMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGFRLNFASPYHL
Homology
BLAST of Carg12968 vs. NCBI nr
Match: KAG7023689.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 399.4 bits (1025), Expect = 1.7e-107
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI
Sbjct: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMGMMPAFTISCSSMIER 120
           TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMGMMPAFTISCSSMIER
Sbjct: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMGMMPAFTISCSSMIER 120

Query: 121 WKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTL 180
           WKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTL
Sbjct: 121 WKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTL 180

Query: 181 YLPGFRLNFASPYHL 196
           YLPGFRLNFASPYHL
Sbjct: 181 YLPGFRLNFASPYHL 195

BLAST of Carg12968 vs. NCBI nr
Match: KAG6590028.1 (Cytochrome P450 72A225, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 388.3 bits (996), Expect = 4.0e-104
Identity = 193/204 (94.61%), Postives = 194/204 (95.10%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI
Sbjct: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------GMMPAFT 120
           TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFG +PRLM         GMMPAFT
Sbjct: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGKNPRLMVKDPEMMKEGMMPAFT 120

Query: 121 ISCSSMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTL 180
           ISCSSMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTL
Sbjct: 121 ISCSSMIERWKEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTL 180

Query: 181 FTVEAMQTLYLPGFRLNFASPYHL 196
           FTVEAMQTLYLPGFRLNFASPYHL
Sbjct: 181 FTVEAMQTLYLPGFRLNFASPYHL 204

BLAST of Carg12968 vs. NCBI nr
Match: XP_022961280.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 352.1 bits (902), Expect = 3.2e-93
Identity = 185/245 (75.51%), Postives = 186/245 (75.92%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI
Sbjct: 14  MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 73

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM                
Sbjct: 74  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKAPEMMKEVLSNKEG 133

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAFTISCS+MIERW
Sbjct: 134 SFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFTISCSNMIERW 193

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY
Sbjct: 194 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 253

BLAST of Carg12968 vs. NCBI nr
Match: XP_023515452.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.1 bits (902), Expect = 3.2e-93
Identity = 185/245 (75.51%), Postives = 186/245 (75.92%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI
Sbjct: 14  MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 73

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM                
Sbjct: 74  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKDPEMMKEVLSNKEG 133

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAFTISCS+MIERW
Sbjct: 134 SFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFTISCSNMIERW 193

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY
Sbjct: 194 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 253

BLAST of Carg12968 vs. NCBI nr
Match: XP_022988287.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 344.7 bits (883), Expect = 5.1e-91
Identity = 181/245 (73.88%), Postives = 183/245 (74.69%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGS+YKPLVGDAKEYVSMI
Sbjct: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSAYKPLVGDAKEYVSMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQI QRVDPFTAVYAKKYGKLSMCWFGTSPRLM                
Sbjct: 61  TEAWSTPMNLDHQITQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKDPEMMKEVLSNKEG 120

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAF ISCS MIERW
Sbjct: 121 SFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFQISCSDMIERW 180

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KEMAGNQESVEV+VWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY
Sbjct: 181 KEMAGNQESVEVEVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 240

BLAST of Carg12968 vs. ExPASy Swiss-Prot
Match: Q9LUC8 (Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.7e-29
Identity = 75/241 (31.12%), Postives = 114/241 (47.30%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+A+ +V + V +    +W +PK++E  L+RQG+ G+ Y PLVGD K   SM+ EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEAR 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+NL   I  R+ P+     K +G+    WFG  P                       
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERW-KEMA 184
                                              ++  M+PAF  SCS ++  W K + 
Sbjct: 128 HTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVT 187

Query: 185 GNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 187
             Q S EVD+WP +  +TADVISR AFGS+++EG+++FEL  +L    ++A +   +PG+
Sbjct: 188 DKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGY 247

BLAST of Carg12968 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.7e-29
Identity = 73/235 (31.06%), Postives = 110/235 (46.81%), Query Frame = 0

Query: 10  ALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAWSTPMN 69
           A+ +V+    + F  +W RP+ +E+ L+ QG  G+SY+   GD KE + M+ EA S P+N
Sbjct: 13  AIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEAKSKPIN 72

Query: 70  LDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP---------------------------- 129
           L   II R+ P        YGK S  W G  P                            
Sbjct: 73  LYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHNPL 132

Query: 130 ------------------------------RLMGMMPAFTISCSSMIERWKEMAGNQESV 187
                                         +L  M+PA  +S S ++ +W+EM   +   
Sbjct: 133 TKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQF 192

BLAST of Carg12968 vs. ExPASy Swiss-Prot
Match: Q9LUC6 (Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 73/240 (30.42%), Postives = 114/240 (47.50%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V +SLA+ +V + V +    +W+ PK++ER L+RQG+ G+SY PL+GD K+ +SM  EA 
Sbjct: 8   VTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEAT 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+     I  RV P      K +G+ ++ WFG  P                       
Sbjct: 68  SKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+  F  SCS ++  W ++  
Sbjct: 128 HTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVS 187

Query: 185 NQ-ESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 186
           ++  S EVDVWP +  +TADVISR AFGS++ EG ++FEL  +L    ++A Q  ++PG+
Sbjct: 188 DKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGY 247

BLAST of Carg12968 vs. ExPASy Swiss-Prot
Match: Q9LUC5 (Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-28
Identity = 71/240 (29.58%), Postives = 115/240 (47.92%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+ L +V + + +    +W++PK++E  L+RQG+ G+ Y PLVGD K+  +M++EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+ L   I  RV P+     K YG+    WFG  P                       
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKP 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+PAF  SC  ++  W ++  
Sbjct: 128 HTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVS 187

Query: 185 NQ-ESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 186
           ++  S EVDVWP +  +TADVISR AFGS+++EG+++FEL  +L    ++A +  ++PG+
Sbjct: 188 DKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGY 247

BLAST of Carg12968 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.3e-28
Identity = 76/237 (32.07%), Postives = 109/237 (45.99%), Query Frame = 0

Query: 8   SLALCLVVFGVA-KAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAWST 67
           ++A+ +VV G A K    +W  P+ +E  L++QG RG+SY+   GD KE   M  +A   
Sbjct: 14  AVAVAVVVVGWAWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMTRKAKLK 73

Query: 68  PMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP------------------------- 127
           P+NL    + RV PF     KKYGK S  W G +P                         
Sbjct: 74  PINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSYKFNKPKR 133

Query: 128 ---------------------------------RLMGMMPAFTISCSSMIERWKEMAGNQ 186
                                            RL  M+PA   SC  M+ +W +M    
Sbjct: 134 NPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKWDKMISKD 193

BLAST of Carg12968 vs. ExPASy TrEMBL
Match: A0A6J1HBQ5 (cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461826 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.5e-93
Identity = 185/245 (75.51%), Postives = 186/245 (75.92%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI
Sbjct: 14  MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 73

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM                
Sbjct: 74  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKAPEMMKEVLSNKEG 133

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAFTISCS+MIERW
Sbjct: 134 SFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFTISCSNMIERW 193

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY
Sbjct: 194 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 253

BLAST of Carg12968 vs. ExPASy TrEMBL
Match: A0A6J1JCN0 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111485582 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.5e-91
Identity = 181/245 (73.88%), Postives = 183/245 (74.69%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGS+YKPLVGDAKEYVSMI
Sbjct: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSAYKPLVGDAKEYVSMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQI QRVDPFTAVYAKKYGKLSMCWFGTSPRLM                
Sbjct: 61  TEAWSTPMNLDHQITQRVDPFTAVYAKKYGKLSMCWFGTSPRLMVKDPEMMKEVLSNKEG 120

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAF ISCS MIERW
Sbjct: 121 SFEKPPMNPLILKLTKGLTTLKGEEWAKHRRIINPAFHLERLKGMMPAFQISCSDMIERW 180

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KEMAGNQESVEV+VWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY
Sbjct: 181 KEMAGNQESVEVEVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 240

BLAST of Carg12968 vs. ExPASy TrEMBL
Match: A0A5D3CDY3 (Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001740 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.1e-82
Identity = 162/245 (66.12%), Postives = 175/245 (71.43%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEY VFYSLALCLVVFGVAKAF+SIWW+PKLVER LKRQG+RG++YKPLVGDAKEYV MI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQI+QRVDPFTAV AKKYGK+SMCWFGTSPRL+                
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAF ISCS+MIE W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKGMMPAFNISCSNMIEDW 180

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KE A NQ+SVEVDVWPE+QRLT DVISRAAFGSNFEEGKK+FE LKQLTL T+EAMQTLY
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTLY 240

BLAST of Carg12968 vs. ExPASy TrEMBL
Match: A0A5A7UKX1 (Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001770 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.1e-82
Identity = 162/245 (66.12%), Postives = 175/245 (71.43%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEY VFYSLALCLVVFGVAKAF+SIWW+PKLVER LKRQG+RG++YKPLVGDAKEYV MI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQI+QRVDPFTAV AKKYGK+SMCWFGTSPRL+                
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                      GMMPAF ISCS+MIE W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKGMMPAFNISCSNMIEDW 180

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KE A NQ+SVEVDVWPE+QRLT DVISRAAFGSNFEEGKK+FE LKQLTL T+EAMQTLY
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTLY 240

BLAST of Carg12968 vs. ExPASy TrEMBL
Match: A0A1S3BRD8 (cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492359 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.7e-81
Identity = 160/245 (65.31%), Postives = 174/245 (71.02%), Query Frame = 0

Query: 1   MEYFVFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMI 60
           MEY VFYSLALCLVVFGVAKAF+SIWW+PKLVER LKRQG+RG++YKPLVGDAKEYV MI
Sbjct: 1   MEYSVFYSLALCLVVFGVAKAFHSIWWKPKLVERHLKRQGIRGNAYKPLVGDAKEYVRMI 60

Query: 61  TEAWSTPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSPRLM---------------- 120
           TEAWSTPMNLDHQI+QRVDPFTAV AKKYGK+SMCWFGTSPRL+                
Sbjct: 61  TEAWSTPMNLDHQIVQRVDPFTAVNAKKYGKISMCWFGTSPRLIVKDPEMMKEVLMNKVG 120

Query: 121 -------------------------------------------GMMPAFTISCSSMIERW 180
                                                       MMPAF ISCS+MIE W
Sbjct: 121 SFEKPPLSPLILKLTRGLTTLKGKEWAKHKRIINPAFHLERLKRMMPAFNISCSNMIEDW 180

Query: 181 KEMAGNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLY 187
           KE A NQ+SVEVDVWPE+QRLT DVISRAAFGSNFEEGKK+FE LKQLTL T+EAMQT+Y
Sbjct: 181 KEKAANQDSVEVDVWPELQRLTGDVISRAAFGSNFEEGKKIFEHLKQLTLLTLEAMQTMY 240

BLAST of Carg12968 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 129.0 bits (323), Expect = 4.1e-30
Identity = 75/241 (31.12%), Postives = 114/241 (47.30%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+A+ +V + V +    +W +PK++E  L+RQG+ G+ Y PLVGD K   SM+ EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEAR 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+NL   I  R+ P+     K +G+    WFG  P                       
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERW-KEMA 184
                                              ++  M+PAF  SCS ++  W K + 
Sbjct: 128 HTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVT 187

Query: 185 GNQESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 187
             Q S EVD+WP +  +TADVISR AFGS+++EG+++FEL  +L    ++A +   +PG+
Sbjct: 188 DKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGY 247

BLAST of Carg12968 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 73/240 (30.42%), Postives = 114/240 (47.50%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V +SLA+ +V + V +    +W+ PK++ER L+RQG+ G+SY PL+GD K+ +SM  EA 
Sbjct: 8   VTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEAT 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+     I  RV P      K +G+ ++ WFG  P                       
Sbjct: 68  SKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+  F  SCS ++  W ++  
Sbjct: 128 HTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVS 187

Query: 185 NQ-ESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 186
           ++  S EVDVWP +  +TADVISR AFGS++ EG ++FEL  +L    ++A Q  ++PG+
Sbjct: 188 DKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGY 247

BLAST of Carg12968 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-29
Identity = 71/240 (29.58%), Postives = 115/240 (47.92%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+ L +V + + +    +W++PK++E  L+RQG+ G+ Y PLVGD K+  +M++EA 
Sbjct: 8   VTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEAR 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+ L   I  RV P+     K YG+    WFG  P                       
Sbjct: 68  SKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKP 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+PAF  SC  ++  W ++  
Sbjct: 128 HTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVS 187

Query: 185 NQ-ESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 186
           ++  S EVDVWP +  +TADVISR AFGS+++EG+++FEL  +L    ++A +  ++PG+
Sbjct: 188 DKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGY 247

BLAST of Carg12968 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 125.2 bits (313), Expect = 5.9e-29
Identity = 73/240 (30.42%), Postives = 116/240 (48.33%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+A+ +V + V +    +W++PK++E  L+RQG+ G+ Y PLVGD K+  SM  EA 
Sbjct: 8   VTVSVAVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEAR 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+NL   I  R+ P+     K +G+    WFG  P                       
Sbjct: 68  SKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+PAF  SCS ++ +W ++  
Sbjct: 128 HTFPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVS 187

Query: 185 NQE-SVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPGF 186
           ++E S EVDVWP +  +TADVISR AFGS+  EG+++FEL  +L    ++ ++  ++PG+
Sbjct: 188 DKESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGY 247

BLAST of Carg12968 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-28
Identity = 72/242 (29.75%), Postives = 114/242 (47.11%), Query Frame = 0

Query: 5   VFYSLALCLVVFGVAKAFYSIWWRPKLVERQLKRQGVRGSSYKPLVGDAKEYVSMITEAW 64
           V  S+ + +V + V +    +W++PK++E  L+RQG+ G+ Y PL+GD K  V+M+TEA 
Sbjct: 8   VTVSVVVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLKRNVNMLTEAT 67

Query: 65  STPMNLDHQIIQRVDPFTAVYAKKYGKLSMCWFGTSP----------------------- 124
           S P+ L   I  RV P      K +G+    W G  P                       
Sbjct: 68  SKPIKLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEVFNKVYDYPKA 127

Query: 125 -----------------------------------RLMGMMPAFTISCSSMIERWKEMAG 184
                                              ++  M+PAF  SCS ++  W ++  
Sbjct: 128 QTFLLGRLIATGIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSDVVGEWSKLVS 187

Query: 185 NQ--ESVEVDVWPEIQRLTADVISRAAFGSNFEEGKKLFELLKQLTLFTVEAMQTLYLPG 187
           ++   S EVDVWP +  +T DVISR AFGS+++EG+++FEL  +L    ++A   +Y+PG
Sbjct: 188 DKGSSSCEVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLILQAFWKVYIPG 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023689.11.7e-107100.00Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6590028.14.0e-10494.61Cytochrome P450 72A225, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961280.13.2e-9375.51cytochrome P450 CYP72A219-like isoform X1 [Cucurbita moschata][more]
XP_023515452.13.2e-9375.51cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022988287.15.1e-9173.88cytochrome P450 CYP72A219-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LUC85.7e-2931.12Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1[more]
H2DH215.7e-2931.06Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q9LUC62.2e-2830.42Cytochrome P450 72A14 OS=Arabidopsis thaliana OX=3702 GN=CYP72A14 PE=2 SV=1[more]
Q9LUC53.7e-2829.58Cytochrome P450 72A15 OS=Arabidopsis thaliana OX=3702 GN=CYP72A15 PE=2 SV=1[more]
A0A0S2IHL26.3e-2832.07Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HBQ51.5e-9375.51cytochrome P450 CYP72A219-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JCN02.5e-9173.88cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111485582 PE=3 ... [more]
A0A5D3CDY36.1e-8266.12Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5A7UKX16.1e-8266.12Cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BRD88.7e-8165.31cytochrome P450 CYP72A219-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349235... [more]
Match NameE-valueIdentityDescription
AT3G14660.14.1e-3031.12cytochrome P450, family 72, subfamily A, polypeptide 13 [more]
AT3G14680.11.5e-2930.42cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
AT3G14690.12.6e-2929.58cytochrome P450, family 72, subfamily A, polypeptide 15 [more]
AT3G14650.15.9e-2930.42cytochrome P450, family 72, subfamily A, polypeptide 11 [more]
AT3G14640.11.0e-2829.75cytochrome P450, family 72, subfamily A, polypeptide 10 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 31..193
e-value: 4.2E-12
score: 47.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 71..189
NoneNo IPR availablePANTHERPTHR24282:SF148CYTOCHROME P450 72A15-LIKEcoord: 102..187
coord: 8..104
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 102..187
coord: 8..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12968-RACarg12968-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen