Carg12936 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12936
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pectinesterase inhibitor 3
Location	Carg_Chr10: 3681565 .. 3682177 (-)
RNA-Seq Expression	Carg12936
Synteny	Carg12936

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCCCTTTTCTCTCCTCCTCGTCCTCCTCCTCTTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTTCTGGCGCCGTCAACACCCCACAAGATCTCCCCAAGCCACCCTCTCCGTCAGCCCTCTCCCTCGCCCGCGACCTCTCCCGGTATCTCTCCAATTCCTCCGCCAACCCTCCGGCAGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGGCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTTTTAAGGAAGTGATGGGGAGGTGAAATTTGATGTTAAGAAGAGGATTTCTAAAGGTCGCTAAAGCTTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

mRNA sequence

ATGACTTCCCTTTTCTCTCCTCCTCGTCCTCCTCCTCTTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTTCTGGCGCCGTCAACACCCCACAAGATCTCCCCAAGCCACCCTCTCCGTCAGCCCTCTCCCTCGCCCGCGACCTCTCCCGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGGCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTCGCTAAAGCTTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

Coding sequence (CDS)

ATGACTTCCCTTTTCTCTCCTCCTCGTCCTCCTCCTCTTCTCTCTCCCTCTCCGCCGCAGCCCACGACGGCCCCTCCGCCGTCCCAAGATCTGATCCACTCCTCCTGCCTTCAAGCCAGCTACCCAAGTCTCTGCATCAGAACCCTCTCCTCCTACTTCTGGCGCCGTCAACACCCCACAAGATCTCCCCAAGCCACCCTCTCCGTCAGCCCTCTCCCTCGCCCGCGACCTCTCCCGGCCTCGAAGCGGCAACGCGCGGCGGTCGACGACTGCGTGGAACAGATTGGAGACTCTGTTGAGGAGCTGAGCAACACTCTGGGTCTCCTCCGCCACCTCCCCTACGGCGACCGGCGGTCTTTCCGATTGCAAATGAGCAATGCCCAGACGTGGGTCAGCGCCGCCTTGACCAATGAAGACACGTGTCTCGATGGGTCGCTAAAGCTTACTAGTAATGCTCTGTATATGATTAATCGCCTCGACGTCGGAACCCCCGCCGGGAGGAAGAAATTGACCGCTCCTTGA

Protein sequence

MTSLFSPPRPPPLLSPSPPQPTTAPPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDGSLKLTSNALYMINRLDVGTPAGRKKLTAP

Homology

[Close]

BLAST of Carg12936 vs. NCBI nr
Match: KAG7023721.1 (Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 343.2 bits (879), Expect = 1.3e-90
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MTSLFSPPRPPPLLSPSPPQPTTAPPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPT 60
           MTSLFSPPRPPPLLSPSPPQPTTAPPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPT
Sbjct: 1   MTSLFSPPRPPPLLSPSPPQPTTAPPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPT 60

Query: 61  RSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSF 120
           RSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSF
Sbjct: 61  RSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSF 120

Query: 121 RLQMSNAQTWVSAALTNEDTCLDGSLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           RLQMSNAQTWVSAALTNEDTCLDGSLKLTSNALYMINRLDVGTPAGRKKLTAP
Sbjct: 121 RLQMSNAQTWVSAALTNEDTCLDGSLKLTSNALYMINRLDVGTPAGRKKLTAP 173

[Close]

BLAST of Carg12936 vs. NCBI nr
Match: XP_022961187.1 (pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023750.1 Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-54
Identity = 130/179 (72.63%), Postives = 130/179 (72.63%), Query Frame = 0


Query: 25  PPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL------ 84
           PPPSQDLIHSSCLQASYPSLCIRTLSSY      P    QATLSVS L   R L      
Sbjct: 24  PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVS-LSLARDLSRYLSN 83

Query: 85  ------PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 144
                  ASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS
Sbjct: 84  SSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 143

Query: 145 AALTNEDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           AALTNEDTCLDG                    K TSNALYMINRLDVGTPAGRKKLTAP
Sbjct: 144 AALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLDVGTPAGRKKLTAP 201

[Close]

BLAST of Carg12936 vs. NCBI nr
Match: XP_023516503.1 (pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.5 bits (563), Expect = 5.7e-54
Identity = 129/179 (72.07%), Postives = 129/179 (72.07%), Query Frame = 0


Query: 25  PPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL------ 84
           PPPSQDLIHSSCLQASYPSLCIRTLSSY      P    QATLSVS L   R L      
Sbjct: 24  PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVS-LSLARDLSRYLSN 83

Query: 85  ------PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 144
                  ASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS
Sbjct: 84  SSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 143

Query: 145 AALTNEDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           AALTNEDTCLDG                    K TSNALYMINRLDVGTPAGRKKL AP
Sbjct: 144 AALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLDVGTPAGRKKLAAP 201

[Close]

BLAST of Carg12936 vs. NCBI nr
Match: XP_022987422.1 (pectinesterase inhibitor 3 [Cucurbita maxima])

HSP 1 Score: 218.0 bits (554), Expect = 6.3e-53
Identity = 127/179 (70.95%), Postives = 128/179 (71.51%), Query Frame = 0


Query: 25  PPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL------ 84
           PPPSQDLIHSSCLQASYPSLCIRTLSSY      P    QATLSVS L   R L      
Sbjct: 24  PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVS-LSLARDLSRYLSN 83

Query: 85  ------PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 144
                  ASKRQR AVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS
Sbjct: 84  SSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 143

Query: 145 AALTNEDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           AALTNEDTCLDG                    K TSNALYMINRL+VGTPAGRKKL AP
Sbjct: 144 AALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLNVGTPAGRKKLAAP 201

[Close]

BLAST of Carg12936 vs. NCBI nr
Match: XP_038879670.1 (pectinesterase inhibitor 3 [Benincasa hispida])

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-42
Identity = 110/174 (63.22%), Postives = 118/174 (67.82%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL--------- 87
           SQDLIHSSCLQA YP+LCIRTLSSY      P    QA +SVS L   R L         
Sbjct: 56  SQDLIHSSCLQARYPNLCIRTLSSYAGAVTTPQELAQAAISVS-LSLARDLSRYLSDALR 115

Query: 88  PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPY-GDRRSFRLQMSNAQTWVSAALTN 147
            AS RQRAAV+DCV+QIGDSVEELSNTLG+LRHL   GDRR FRLQM NA+TWVSAALTN
Sbjct: 116 QASMRQRAAVNDCVDQIGDSVEELSNTLGVLRHLTCGGDRRDFRLQMGNAKTWVSAALTN 175

Query: 148 EDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           EDTCLDG                    K+TSNAL+MINRLD G PAGRKKL AP
Sbjct: 176 EDTCLDGFKEVDGEVKLDVKKRISKVAKVTSNALFMINRLDGGNPAGRKKLAAP 228

[Close]

BLAST of Carg12936 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 75/165 (45.45%), Postives = 100/165 (60.61%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFW-----RRQHPTRSPQATLSVSPLPRPR------ 87
           ++D++HSSC  ASYPSLC+RTLSSY       RR     + + +LS +     +      
Sbjct: 30  AEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRD 89

Query: 88  PLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPY--GDRRSFRLQMSNAQTWVSAA 147
            +   K+++AA+ DCVE IGDSV+ELS TLG+L+HL    G  + FR QMSNAQTW SAA
Sbjct: 90  SVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAA 149

Query: 148 LTNEDTCLDG-------------------SLKLTSNALYMINRLD 161
           LT++DTCLDG                     ++TSNALYM+N+LD
Sbjct: 150 LTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQLD 194

[Close]

BLAST of Carg12936 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.2e-15
Identity = 50/128 (39.06%), Postives = 73/128 (57.03%), Query Frame = 0


Query: 30  DLIHSSCLQASYPSLCIRTLSSYFWR-RQHPTRSPQATLSVSPLPRPRPLPA-------- 89
           + I +SC   SYP  C ++LSSY    ++ P +  +  L+VS + R +            
Sbjct: 36  EFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVS-IARAKSAKTYVSEMTDY 95

Query: 90  ---SKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDR-RSFRLQMSNAQTWVSAALT 145
              +KRQ  AV DC+E++GD+V+ LSN+L  L+HL  GD    F   +SN +TW SAALT
Sbjct: 96  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 155

[Close]

BLAST of Carg12936 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.5e-14
Identity = 46/131 (35.11%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 27  PSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHP-------------TRSPQATLSVSPLP 86
           P+   I +SC   +YP++C ++LS+Y    Q+              TR+ QA   +  L 
Sbjct: 24  PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLN 83

Query: 87  RPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSA 145
           + + L A  RQ AA+ DC+E++ DS++ +S +   +++L +     F  +MSN +TWVSA
Sbjct: 84  KFKGLKA--RQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSA 143

[Close]

BLAST of Carg12936 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.0e-13
Identity = 61/184 (33.15%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 13  LLSPSPPQPTTAPP---PSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHP---------- 72
           LLS +  Q ++A P    S   I SSC    YPSLC+ TLS+Y  + +H           
Sbjct: 12  LLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTAL 71

Query: 73  ----TRSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYG 132
                R+   T+ V+ L +  P    +R+  A+ DC+E +G+SV+ L+ ++  L    + 
Sbjct: 72  IISLARAKSVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHA 131

Query: 133 -DRRSFRLQMSNAQTWVSAALTNEDTCLDG--------------------SLKLTSNALY 159
                F  +MSN QTWVSAALT+E TCLDG                      ++TSNAL 
Sbjct: 132 VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALA 191

[Close]

BLAST of Carg12936 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.1e-13
Identity = 70/206 (33.98%), Postives = 92/206 (44.66%), Query Frame = 0


Query: 3   SLFSPPRPPPL-LSPSPPQPTTAPPPSQ---------------------DLIHSSCLQAS 62
           S  SP  PPPL LSPS P P   PPPS                      D I +SC    
Sbjct: 100 SSLSPSSPPPLSLSPSSPPP---PPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITL 159

Query: 63  YPSLCIRTLSSYFWR-RQHPTRSPQATLSVSPLPRPRPLPASKR----------QRAAVD 122
           Y ++C  +LS Y    R +P +     L+++           K           +  AV 
Sbjct: 160 YKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVA 219

Query: 123 DCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDG----- 158
           DCVE+IGDSV  L +++  L  + Y D   F + MS+ +TWVSAALTN+DTC+DG     
Sbjct: 220 DCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVK 279

[Close]

BLAST of Carg12936 vs. ExPASy TrEMBL
Match: A0A6J1H9I1 (pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 7.3e-55
Identity = 130/179 (72.63%), Postives = 130/179 (72.63%), Query Frame = 0


Query: 25  PPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL------ 84
           PPPSQDLIHSSCLQASYPSLCIRTLSSY      P    QATLSVS L   R L      
Sbjct: 24  PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVS-LSLARDLSRYLSN 83

Query: 85  ------PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 144
                  ASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS
Sbjct: 84  SSANLRQASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 143

Query: 145 AALTNEDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           AALTNEDTCLDG                    K TSNALYMINRLDVGTPAGRKKLTAP
Sbjct: 144 AALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLDVGTPAGRKKLTAP 201

[Close]

BLAST of Carg12936 vs. ExPASy TrEMBL
Match: A0A6J1JAB3 (pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.1e-53
Identity = 127/179 (70.95%), Postives = 128/179 (71.51%), Query Frame = 0


Query: 25  PPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL------ 84
           PPPSQDLIHSSCLQASYPSLCIRTLSSY      P    QATLSVS L   R L      
Sbjct: 24  PPPSQDLIHSSCLQASYPSLCIRTLSSYSGAVNTPQDLAQATLSVS-LSLARDLSRYLSN 83

Query: 85  ------PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 144
                  ASKRQR AVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS
Sbjct: 84  SSVNLRQASKRQRVAVDDCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVS 143

Query: 145 AALTNEDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKLTAP 174
           AALTNEDTCLDG                    K TSNALYMINRL+VGTPAGRKKL AP
Sbjct: 144 AALTNEDTCLDGFKEVDGEVKFDVKKRISKVAKATSNALYMINRLNVGTPAGRKKLAAP 201

[Close]

BLAST of Carg12936 vs. ExPASy TrEMBL
Match: A0A0A0LWR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 9.3e-42
Identity = 106/171 (61.99%), Postives = 119/171 (69.59%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL--------- 87
           SQDLIHSSCLQASYP+LCIRTLSSY    + P    QAT+SVS L   + L         
Sbjct: 53  SQDLIHSSCLQASYPTLCIRTLSSYAGAVKTPRDLAQATISVS-LSLAQNLSEYLSDSLR 112

Query: 88  PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYG-DRRSFRLQMSNAQTWVSAALTN 147
            AS++QRAAVDDCV+QIGDSVEELSNTLG+LRHLP G DRR FRL+M NA+TWVSAALTN
Sbjct: 113 QASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLPCGDDRRKFRLEMGNAKTWVSAALTN 172

Query: 148 EDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRKKL 171
           E+TCLDG                    K+TSNAL+MINRLD G P GRK L
Sbjct: 173 EETCLDGFKEVDGEVKLDVKRRILKVAKVTSNALFMINRLDGGNPTGRKML 222

[Close]

BLAST of Carg12936 vs. ExPASy TrEMBL
Match: A0A5A7UKZ9 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 8.1e-38
Identity = 99/169 (58.58%), Postives = 114/169 (67.46%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL--------- 87
           SQDLI SSCLQA YP+LCIRTLSSY    + P    Q T+SVS L   + L         
Sbjct: 27  SQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRDLAQVTISVS-LSLAQNLSEYLSDSLR 86

Query: 88  PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYG-DRRSFRLQMSNAQTWVSAALTN 147
            AS++QRAAVDDCV+QIGDSVEELSNTLG+LRHLP G DRR FRL+M NA+TWVSAALTN
Sbjct: 87  KASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLPCGDDRRKFRLEMGNAKTWVSAALTN 146

Query: 148 EDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRK 169
           E+TCLDG                    K+TSNAL+MINRLD G   G++
Sbjct: 147 EETCLDGFKEVDGEVKLDVKRRIVKVAKVTSNALFMINRLDSGNSTGKE 194

[Close]

BLAST of Carg12936 vs. ExPASy TrEMBL
Match: A0A1S3BR62 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 8.1e-38
Identity = 99/169 (58.58%), Postives = 114/169 (67.46%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFWRRQHPTRSPQATLSVSPLPRPRPL--------- 87
           SQDLI SSCLQA YP+LCIRTLSSY    + P    Q T+SVS L   + L         
Sbjct: 27  SQDLIRSSCLQARYPTLCIRTLSSYAGSVKTPRDLAQVTISVS-LSLAQNLSEYLSDSLR 86

Query: 88  PASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYG-DRRSFRLQMSNAQTWVSAALTN 147
            AS++QRAAVDDCV+QIGDSVEELSNTLG+LRHLP G DRR FRL+M NA+TWVSAALTN
Sbjct: 87  KASRQQRAAVDDCVDQIGDSVEELSNTLGVLRHLPCGDDRRKFRLEMGNAKTWVSAALTN 146

Query: 148 EDTCLDG------------------SLKLTSNALYMINRLDVGTPAGRK 169
           E+TCLDG                    K+TSNAL+MINRLD G   G++
Sbjct: 147 EETCLDGFKEVDGEVKLDVKRRIVKVAKVTSNALFMINRLDSGNSTGKE 194

[Close]

BLAST of Carg12936 vs. TAIR 10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 8.9e-29
Identity = 75/165 (45.45%), Postives = 100/165 (60.61%), Query Frame = 0


Query: 28  SQDLIHSSCLQASYPSLCIRTLSSYFW-----RRQHPTRSPQATLSVSPLPRPR------ 87
           ++D++HSSC  ASYPSLC+RTLSSY       RR     + + +LS +     +      
Sbjct: 30  AEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRD 89

Query: 88  PLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPY--GDRRSFRLQMSNAQTWVSAA 147
            +   K+++AA+ DCVE IGDSV+ELS TLG+L+HL    G  + FR QMSNAQTW SAA
Sbjct: 90  SVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAA 149

Query: 148 LTNEDTCLDG-------------------SLKLTSNALYMINRLD 161
           LT++DTCLDG                     ++TSNALYM+N+LD
Sbjct: 150 LTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQLD 194

[Close]

BLAST of Carg12936 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 3.0e-16
Identity = 50/128 (39.06%), Postives = 73/128 (57.03%), Query Frame = 0


Query: 30  DLIHSSCLQASYPSLCIRTLSSYFWR-RQHPTRSPQATLSVSPLPRPRPLPA-------- 89
           + I +SC   SYP  C ++LSSY    ++ P +  +  L+VS + R +            
Sbjct: 36  EFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVS-IARAKSAKTYVSEMTDY 95

Query: 90  ---SKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYGDR-RSFRLQMSNAQTWVSAALT 145
              +KRQ  AV DC+E++GD+V+ LSN+L  L+HL  GD    F   +SN +TW SAALT
Sbjct: 96  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 155

[Close]

BLAST of Carg12936 vs. TAIR 10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.3e-15
Identity = 58/137 (42.34%), Postives = 78/137 (56.93%), Query Frame = 0


Query: 18  PPQPTTAPPPSQDLIHSSCLQASYPSLCIRTLSSYFWRRQ-HPTRSPQATLSVSPLPRPR 77
           P QPTT      D I +SC    YP +C  +L+ Y    Q +P R  +  + VS L R +
Sbjct: 48  PEQPTT---DDLDFIRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVS-LSRAK 107

Query: 78  PLPA--SKRQR----AAVDDCVEQIGDSVEELSNTLGLLRHLPY---GDRRSFRLQMSNA 137
              A  SK  R    AAV DCV  +GD+V+++  +L  LR + +   GD  +FR QMSN 
Sbjct: 108 YTAAYLSKLSRRAASAAVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGD-PAFRFQMSNV 167

Query: 138 QTWVSAALTNEDTCLDG 145
           QTW+SAALT+E+TC DG
Sbjct: 168 QTWMSAALTDEETCTDG 179

[Close]

BLAST of Carg12936 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-14
Identity = 61/184 (33.15%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 13  LLSPSPPQPTTAPP---PSQDLIHSSCLQASYPSLCIRTLSSYFWRRQHP---------- 72
           LLS +  Q ++A P    S   I SSC    YPSLC+ TLS+Y  + +H           
Sbjct: 12  LLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTAL 71

Query: 73  ----TRSPQATLSVSPLPRPRPLPASKRQRAAVDDCVEQIGDSVEELSNTLGLLRHLPYG 132
                R+   T+ V+ L +  P    +R+  A+ DC+E +G+SV+ L+ ++  L    + 
Sbjct: 72  IISLARAKSVTIFVAKLTKETP-KFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHA 131

Query: 133 -DRRSFRLQMSNAQTWVSAALTNEDTCLDG--------------------SLKLTSNALY 159
                F  +MSN QTWVSAALT+E TCLDG                      ++TSNAL 
Sbjct: 132 VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALA 191

[Close]

BLAST of Carg12936 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 3.6e-14
Identity = 70/206 (33.98%), Postives = 92/206 (44.66%), Query Frame = 0


Query: 3   SLFSPPRPPPL-LSPSPPQPTTAPPPSQ---------------------DLIHSSCLQAS 62
           S  SP  PPPL LSPS P P   PPPS                      D I +SC    
Sbjct: 100 SSLSPSSPPPLSLSPSSPPP---PPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITL 159

Query: 63  YPSLCIRTLSSYFWR-RQHPTRSPQATLSVSPLPRPRPLPASKR----------QRAAVD 122
           Y ++C  +LS Y    R +P +     L+++           K           +  AV 
Sbjct: 160 YKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVA 219

Query: 123 DCVEQIGDSVEELSNTLGLLRHLPYGDRRSFRLQMSNAQTWVSAALTNEDTCLDG----- 158
           DCVE+IGDSV  L +++  L  + Y D   F + MS+ +TWVSAALTN+DTC+DG     
Sbjct: 220 DCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFSLVK 279

The following BLAST results are available for this feature:

BLAST of Carg12936 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023721.1	1.3e-90	100.00	Pectinesterase inhibitor 3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022961187.1	1.5e-54	72.63	pectinesterase inhibitor 3 [Cucurbita moschata] >KAG6590055.1 Pectinesterase inh...	[more]
XP_023516503.1	5.7e-54	72.07	pectinesterase inhibitor 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022987422.1	6.3e-53	70.95	pectinesterase inhibitor 3 [Cucurbita maxima]	[more]
XP_038879670.1	1.0e-42	63.22	pectinesterase inhibitor 3 [Benincasa hispida]	[more]

BLAST of Carg12936 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WE4	1.3e-27	45.45	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9SB37	4.2e-15	39.06	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	3.5e-14	35.11	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI72	3.0e-13	33.15	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SI74	5.1e-13	33.98	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Carg12936 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9I1	7.3e-55	72.63	pectinesterase inhibitor 3 OS=Cucurbita moschata OX=3662 GN=LOC111461775 PE=4 SV...	[more]
A0A6J1JAB3	3.1e-53	70.95	pectinesterase inhibitor 3 OS=Cucurbita maxima OX=3661 GN=LOC111484974 PE=4 SV=1	[more]
A0A0A0LWR8	9.3e-42	61.99	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G597770 PE=4 S...	[more]
A0A5A7UKZ9	8.1e-38	58.58	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001300...	[more]
A0A1S3BR62	8.1e-38	58.58	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492396 PE=4 SV=1	[more]

BLAST of Carg12936 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20740.1	8.9e-29	45.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	3.0e-16	39.06	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G14890.1	3.3e-15	42.34	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.1e-14	33.15	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	3.6e-14	33.98	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 27..155 e-value: 6.9E-16 score: 68.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 81..144 e-value: 7.0E-11 score: 39.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..145 e-value: 4.5E-18 score: 66.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..161 e-value: 2.9E-18 score: 68.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 29..159
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 63..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 27..160
None	No IPR available	PANTHER	PTHR31080:SF110	PECTINESTERASE INHIBITOR 3	coord: 27..160
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 34..160 e-value: 1.7148E-25 score: 93.6569

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12936-RA	Carg12936-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity