Carg12880 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12880
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AvrRpt-cleavage domain-containing protein
Location	Carg_Chr05: 10498580 .. 10499495 (-)
RNA-Seq Expression	Carg12880
Synteny	Carg12880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGTCGCCTCCCTCTCTCTCTCTCAATCCGTCAGTGTTGTTTTCTCCCTTCGATTGATTTTGAAATTCCATTTTTTTTTTCCTCGGGAGCGTTTCTCTCTCCAGAATTCATGACGGTATGTTCTGGTTTGATTTCTGCGTCTTAAGAATATTATTACAATTTTCATTTTCAGCTTTGATTTTTTCTTTGATGTGCTTTCCGATCGGTTCGAGTTTTGCTTCCTATTGAGTTTTCGTTTGTTTGATTCGAATCGCCACTTTTGATTTGTTTATTCATTTGATTTTGTTAAAGATTGTTCAATTCTGCGTTTCTAGATTTTTTTTTTTCTTTCGTTTTCCTGAGATTGATGTTGATTGATTCTGGATTAGTTAAATTGGGCAGAAATCTGGATTTTTGTGCAAGATTAATTAATTTCTTGGAGGTCGTTTCTGAATGAATAATTCTGACAGCTGCATTCAAATATGTGACGTAGTTGGAGTAATTGATGTTGGAGGAAACTTTTTAATTGGTCTATTTTGTTTTGTTTGATTGAGTAATATCGATATTTTCAACATCTGTTGCTATAAACCAATGTTGTATGGTAATAAATGATGGTTGTTAGATTCGTAGTCCGATTGGTAAGGTAAGCCATCAGATTCACCGAGGGTACATCTTGTTTGTTCTTTCTTTTTGTAGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

mRNA sequence

AAAGTCGCCTCCCTCTCTCTCTCTCAATCCGTCAGTGTTGTTTTCTCCCTTCGATTGATTTTGAAATTCCATTTTTTTTTTCCTCGGGAGCGTTTCTCTCTCCAGAATTCATGACGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

Coding sequence (CDS)

ATGACGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

Protein sequence

MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAKAPPVRYIHNPFSFVLLNGGYS

Homology

[Close]

BLAST of Carg12880 vs. NCBI nr
Match: KAG7030269.1 (RPM1-interacting protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 174.1 bits (440), Expect = 4.9e-40
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRYIHNPFSFVLLNGGYS 82
          APPVRYIHNPFSFVLLNGGYS
Sbjct: 61 APPVRYIHNPFSFVLLNGGYS 81

[Close]

BLAST of Carg12880 vs. NCBI nr
Match: XP_008463014.1 (PREDICTED: RPM1-interacting protein 4-like isoform X1 [Cucumis melo])

HSP 1 Score: 145.2 bits (365), Expect = 2.4e-31
Identity = 68/78 (87.18%), Postives = 70/78 (89.74%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRTAPDPAK 60

Query: 61 APPVRYIHNPFSFVLLNG 79
          APPVRYIHN F    L+G
Sbjct: 61 APPVRYIHNLFPIWALDG 78

[Close]

BLAST of Carg12880 vs. NCBI nr
Match: XP_022975420.1 (uncharacterized protein LOC111474733 isoform X1 [Cucurbita maxima])

HSP 1 Score: 139.0 bits (349), Expect = 1.8e-29
Identity = 67/79 (84.81%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRY---IHNPFSFVLL 77
          APP ++   I + F F+ L
Sbjct: 61 APPKKWLCCIQSLFDFIYL 79

[Close]

BLAST of Carg12880 vs. NCBI nr
Match: XP_023535777.1 (uncharacterized protein LOC111797108 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 139.0 bits (349), Expect = 1.8e-29
Identity = 67/79 (84.81%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRY---IHNPFSFVLL 77
          APP ++   I + F F+ L
Sbjct: 61 APPKKWLCCIQSLFDFIYL 79

[Close]

BLAST of Carg12880 vs. NCBI nr
Match: XP_022936748.1 (uncharacterized protein LOC111443250 [Cucurbita moschata] >XP_022975427.1 uncharacterized protein LOC111474733 isoform X2 [Cucurbita maxima] >XP_023535778.1 uncharacterized protein LOC111797108 isoform X2 [Cucurbita pepo subsp. pepo] >KAG6592101.1 Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.7 bits (343), Expect = 8.7e-29
Identity = 63/67 (94.03%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

[Close]

BLAST of Carg12880 vs. ExPASy Swiss-Prot
Match: O22633 (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.8e-21
Identity = 50/70 (71.43%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGAD 60
          M+DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G D
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGD 60

Query: 61 PAKAPPVRYI 68
          P+K  P +++
Sbjct: 61 PSKPQPKKWL 69

[Close]

BLAST of Carg12880 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.3e-06
Identity = 20/37 (54.05%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 3   DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTG 40
           +K   +PKFG+WD N+P+SA+G+T IFNK R+E+ +G
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSG 179

[Close]

BLAST of Carg12880 vs. ExPASy TrEMBL
Match: A0A1S3CI83 (RPM1-interacting protein 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501257 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.2e-31
Identity = 68/78 (87.18%), Postives = 70/78 (89.74%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRTAPDPAK 60

Query: 61 APPVRYIHNPFSFVLLNG 79
          APPVRYIHN F    L+G
Sbjct: 61 APPVRYIHNLFPIWALDG 78

[Close]

BLAST of Carg12880 vs. ExPASy TrEMBL
Match: A0A6J1IGN4 (uncharacterized protein LOC111474733 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111474733 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.5e-30
Identity = 67/79 (84.81%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRY---IHNPFSFVLL 77
          APP ++   I + F F+ L
Sbjct: 61 APPKKWLCCIQSLFDFIYL 79

[Close]

BLAST of Carg12880 vs. ExPASy TrEMBL
Match: A0A6J1ID15 (uncharacterized protein LOC111474733 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111474733 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-29
Identity = 63/67 (94.03%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

[Close]

BLAST of Carg12880 vs. ExPASy TrEMBL
Match: A0A6J1FE40 (uncharacterized protein LOC111443250 OS=Cucurbita moschata OX=3662 GN=LOC111443250 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.2e-29
Identity = 63/67 (94.03%), Postives = 66/67 (98.51%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

[Close]

BLAST of Carg12880 vs. ExPASy TrEMBL
Match: A0A6J1CEA3 (RPM1-interacting protein 4 OS=Momordica charantia OX=3673 GN=LOC111010436 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.0e-27
Identity = 60/67 (89.55%), Postives = 65/67 (97.01%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA+GR GADP+K
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDARGRPGADPSK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

[Close]

BLAST of Carg12880 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 103.6 bits (257), Expect = 7.6e-23
Identity = 51/68 (75.00%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGAD 60
          M+DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G D
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGD 60

Query: 61 PAKAPPVR 66
          P+K  PV+
Sbjct: 61 PSKPQPVK 67

[Close]

BLAST of Carg12880 vs. TAIR 10
Match: AT5G55850.2 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 100.9 bits (250), Expect = 4.9e-22
Identity = 50/66 (75.76%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGADPA 62
          DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G DP+
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGDPS 78

Query: 63 KAPPVR 66
          K  PV+
Sbjct: 79 KPQPVK 83

[Close]

BLAST of Carg12880 vs. TAIR 10
Match: AT5G55850.3 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 100.1 bits (248), Expect = 8.4e-22
Identity = 49/68 (72.06%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGADPA 62
          DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G DP+
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGDPS 78

Query: 63 KAPPVRYI 68
          K  P +++
Sbjct: 79 KPQPKKWL 85

[Close]

BLAST of Carg12880 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-19
Identity = 42/47 (89.36%), Postives = 44/47 (93.62%), Query Frame = 0


Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGK 48
          M DKGRPLPKFGEWDVNDP+SAEGFTVIFNKAR+EKK GGK DSPGK
Sbjct: 1  MADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGGKSDSPGK 47

[Close]

BLAST of Carg12880 vs. TAIR 10
Match: AT5G63270.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 68.9 bits (167), Expect = 2.1e-12
Identity = 33/46 (71.74%), Postives = 38/46 (82.61%), Query Frame = 0


Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKT----GGKPDS 45
          +KG+PLPKFGEWDVN+P SAEGFTVIF+KA DEKKT    G  P+S
Sbjct: 4  NKGKPLPKFGEWDVNNPASAEGFTVIFSKASDEKKTKKASGAGPNS 49

The following BLAST results are available for this feature:

BLAST of Carg12880 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030269.1	4.9e-40	100.00	RPM1-interacting protein 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_008463014.1	2.4e-31	87.18	PREDICTED: RPM1-interacting protein 4-like isoform X1 [Cucumis melo]	[more]
XP_022975420.1	1.8e-29	84.81	uncharacterized protein LOC111474733 isoform X1 [Cucurbita maxima]	[more]
XP_023535777.1	1.8e-29	84.81	uncharacterized protein LOC111797108 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022936748.1	8.7e-29	94.03	uncharacterized protein LOC111443250 [Cucurbita moschata] >XP_022975427.1 unchar...	[more]

BLAST of Carg12880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22633	1.8e-21	71.43	Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1	[more]
Q8GYN5	6.3e-06	54.05	RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1	[more]

BLAST of Carg12880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CI83	1.2e-31	87.18	RPM1-interacting protein 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035012...	[more]
A0A6J1IGN4	8.5e-30	84.81	uncharacterized protein LOC111474733 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1ID15	4.2e-29	94.03	uncharacterized protein LOC111474733 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FE40	4.2e-29	94.03	uncharacterized protein LOC111443250 OS=Cucurbita moschata OX=3662 GN=LOC1114432...	[more]
A0A6J1CEA3	1.0e-27	89.55	RPM1-interacting protein 4 OS=Momordica charantia OX=3673 GN=LOC111010436 PE=4 S...	[more]

BLAST of Carg12880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55850.1	7.6e-23	75.00	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.2	4.9e-22	75.76	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.3	8.4e-22	72.06	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT2G04410.1	1.8e-19	89.36	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G63270.1	2.1e-12	71.74	RPM1-interacting protein 4 (RIN4) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008700	RIN4, pathogenic type III effector avirulence factor Avr cleavage site	PFAM	PF05627	AvrRpt-cleavage	coord: 3..38 e-value: 9.2E-23 score: 79.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..63
None	No IPR available	PANTHER	PTHR33159:SF59	RIN4, PATHOGENIC TYPE III EFFECTOR AVIRULENCE FACTOR AVR CLEAVAGE SITE-RELATED	coord: 3..68
IPR040387	RPM1-interacting protein 4/NOI4	PANTHER	PTHR33159	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 3..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12880-RA	Carg12880-RA	mRNA