Carg12835 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12835
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	GDP-L-galactose phosphorylase 1-like
Location	Carg_Chr09: 5413993 .. 5416376 (+)
RNA-Seq Expression	Carg12835
Synteny	Carg12835

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TACGACGAAAGGGTTGTATAACTCTTACTAACCCCTCGCCGCACGGTGGACGTGGTGCTTTGCCCTCCGAAGGGGGTAGTCCTTCTGATCTTCTTTTTCTTGCCGGCGGTGGATTCTCTTGCGTAGAGCATTCTTCGAATTAGCTTTTCAACGGCTTTGTTTGTTTTGGGGGGCCGATTTTGGTGTTGGGTTTGATTTGAATTAGAAGCGGACGATATGTTGAGGATTAAGCGTGTTCCGACTGTGGTATCTAATTTCCAAAAGGATGAAGCTGAGGAAGTCGCTCCCAGAGCAGGGTGCGGCCGGAACTGCCTCAATGAATGTTGCTATGAAGGTGTTTGTTAAAATGCGTAGCTAGCGAGGATTCGTTTGGTTGCTTCTCTTGTGTTCTTGATTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGGTCTGAAATGTTTTCGTTCCATGTGGGTATTGGGGCTGTAATCATGATTGTTCGTTTCCCTTTTGTTGTAGGAGCCAAGATTCCCTTGTATGCTTTCAAGAAGCTAAACAAGATCTCAAAAGGCTTGTGCTGCGAGTATGAGAACACTGCACCTCCTTTTGCTTTTCTTGACTCGCTGCTTCTTGGGGAGGTAAGTTTGATGACATGCTCTGGTTATCAATTTTGCTTATCTTTCAATGGCTATAATGGATGCCCCGTTGTTGTAGTGGGAGGACCGGATGCAGCGAGGGCTGTTTCGCTATGATGTCACTGCTTGTGAGACTAAGGTAAGAATTCTACAGTAATTTGTGAAATAAAAGTTGATAGGCGAAGTAATCTGCTATTTGAGATATAGATGTAGTCTCAATGTGAGATCCCACGATTGGTTGGGGAGGAGAACGAAGCACTCTTTACAAGGGTGTGGGAACGAAGCACTCTTTACAAGGGTGTGGGAACGAAGCACTCTTTACAAGGGTGTGGGAACGAAGCACTCTTTACAAGGGTGTGGGAACGAAGCACTCTTTACAAGGGGGGAAGCCCTCGTTTGGGAAAGTCTAGGACAATATCGGCTGGCAGTAGGTCTGAGCTGTTACACTCAATATCTTTGGTAACTTTTGTGTAGGTAATTCCTGGAAAGTATGGTTTCATTGCCCAGCTGAACGAGGGTCGACATCTGAAGAAGAGGCCAACCGAGTTTCGAGTTGATAAGGTTCTCCAGCCTTTTGATGGTAACAAATTCAACTTCACTAAGGTTGGGCAAGAAGAGATCCTCTTTCGGTTTGAAGCAAATGAAAATGGGAATACACAGCTTATTCCAAATGCCCCTCTTGATGTCGAAAATTCTCCAAGCATGGTTGCCATTAATGTGTGTACTTGTTCCTGTTTTAGTGTTTTTAGACCAAATTGTTGGGGATGTTTGTGTTGTGATCATTCTTAATATGTCATCAACAGGTTAGTCCCATTGAATACGGACACGTCCTCTTGATTCCTCGTATTTTTGAGCGCTTGCCACAAAGGATCGATCGAGAAAGCTTCTTGCTTGCTCTTCACATGGCAGCGGAGGCTGGGAATACATACTTCCGACTCGGGTACAACAGCTTGGGCGCATTCGCCACCATCAATCACCTTCACTTTCAGGTATCTATCTACCCTTTAGAAAAAGTGACTTGATGAAGCTAGTCTTTACACAAGAACATGTGAATTAATAACTAAATAATCAATCAGGCATATTACTTGGCTGTTCCTTTCCCTATTGAGAAAGCTCCGACGAAGAAAACAATCTCGTTGAATGATGAAGTTATCGTCTCCAAGCTACTAAAATATCCCGTTCGAGGGCTCGTGTTCGAGGGCGAAAACACTCTTCAAGCGTTATCGAACGCGGTGTCTGATGCCTGCATATGCCTTCAAGAAAACAACATTCCTTACAATGTCTTAATTGCTGATTGTGGGCAACGTATCTTCCTTCTCCCACAGGCAAGCTGATATTATCTGATGCCAGCATTAGCTAGCTCTTAGTTTCCTGTATCCTTGTTAACTCTGACCTTACCTATCATAACTAACAGTGCTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGTGGAGCTCCTCGACACCCAAGTGAACCCGGCCGTTTGGGAGATAAGTGGGCACATGGTGTTGAAGAGGAAGAAGGATTACGACGAGGCGTCGGAGGAAAACGCCTGGAGGCTCCTTGCTGAGGTATCCCTATCAGAAGAGAGGTTTCAAGAAGTGTGTGCTCTAATCTTTGAAGTCCTCGACACAGTTGATGTTGAAGGTAGCCCGAGCGTGGCTGAGGAGCACGGTGATGCTTGTCTCGAGGAAGTTGAAGCTAGAAACAACGCCTCCCGACCACTCCGGGTGACTGGGACGTGGGAATGCTTAGTTTAG

mRNA sequence

TACGACGAAAGGGTTGTATAACTCTTACTAACCCCTCGCCGCACGGTGGACGTGGTGCTTTGCCCTCCGAAGGGGGTAGTCCTTCTGATCTTCTTTTTCTTGCCGGCGGTGGATTCTCTTGCGTAGAGCATTCTTCGAATTAGCTTTTCAACGGCTTTGTTTGTTTTGGGGGGCCGATTTTGGTGTTGGGTTTGATTTGAATTAGAAGCGGACGATATGTTGAGGATTAAGCGTGTTCCGACTGTGGTATCTAATTTCCAAAAGGATGAAGCTGAGGAAGTCGCTCCCAGAGCAGGGTGCGGCCGGAACTGCCTCAATGAATGTTGCTATGAAGGAGCCAAGATTCCCTTGTATGCTTTCAAGAAGCTAAACAAGATCTCAAAAGGCTTGTGCTGCGAGTATGAGAACACTGCACCTCCTTTTGCTTTTCTTGACTCGCTGCTTCTTGGGGAGAGGCCAACCGAGTTTCGAGTTGATAAGGTTCTCCAGCCTTTTGATGGTAACAAATTCAACTTCACTAAGGTTGGGCAAGAAGAGATCCTCTTTCGGTTTGAAGCAAATGAAAATGGGAATACACAGCTTATTCCAAATGCCCCTCTTGATGTCGAAAATTCTCCAAGCATGGTTGCCATTAATGTTAGTCCCATTGAATACGGACACGTCCTCTTGATTCCTCGTATTTTTGAGCGCTTGCCACAAAGGATCGATCGAGAAAGCTTCTTGCTTGCTCTTCACATGGCAGCGGAGGCTGGGAATACATACTTCCGACTCGGGTACAACAGCTTGGGCGCATTCGCCACCATCAATCACCTTCACTTTCAGGCATATTACTTGGCTGTTCCTTTCCCTATTGAGAAAGCTCCGACGAAGAAAACAATCTCGTTGAATGATGAAGTTATCGTCTCCAAGCTACTAAAATATCCCGTTCGAGGGCTCGTGTTCGAGGGCGAAAACACTCTTCAAGCGTTATCGAACGCGGTGTCTGATGCCTGCATATGCCTTCAAGAAAACAACATTCCTTACAATTGCTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGTGGAGCTCCTCGACACCCAAGTGAACCCGGCCGTTTGGGAGATAAGTGGGCACATGGTGTTGAAGAGGAAGAAGGATTACGACGAGGCGTCGGAGGAAAACGCCTGGAGGCTCCTTGCTGAGGTATCCCTATCAGAAGAGAGGTTTCAAGAAGTGTGTGCTCTAATCTTTGAAGTCCTCGACACAGTTGATGTTGAAGGTAGCCCGAGCGTGGCTGAGGAGCACGGTGATGCTTGTCTCGAGGAAGTTGAAGCTAGAAACAACGCCTCCCGACCACTCCGGGTGACTGGGACGTGGGAATGCTTAGTTTAG

Coding sequence (CDS)

ATGTTGAGGATTAAGCGTGTTCCGACTGTGGTATCTAATTTCCAAAAGGATGAAGCTGAGGAAGTCGCTCCCAGAGCAGGGTGCGGCCGGAACTGCCTCAATGAATGTTGCTATGAAGGAGCCAAGATTCCCTTGTATGCTTTCAAGAAGCTAAACAAGATCTCAAAAGGCTTGTGCTGCGAGTATGAGAACACTGCACCTCCTTTTGCTTTTCTTGACTCGCTGCTTCTTGGGGAGAGGCCAACCGAGTTTCGAGTTGATAAGGTTCTCCAGCCTTTTGATGGTAACAAATTCAACTTCACTAAGGTTGGGCAAGAAGAGATCCTCTTTCGGTTTGAAGCAAATGAAAATGGGAATACACAGCTTATTCCAAATGCCCCTCTTGATGTCGAAAATTCTCCAAGCATGGTTGCCATTAATGTTAGTCCCATTGAATACGGACACGTCCTCTTGATTCCTCGTATTTTTGAGCGCTTGCCACAAAGGATCGATCGAGAAAGCTTCTTGCTTGCTCTTCACATGGCAGCGGAGGCTGGGAATACATACTTCCGACTCGGGTACAACAGCTTGGGCGCATTCGCCACCATCAATCACCTTCACTTTCAGGCATATTACTTGGCTGTTCCTTTCCCTATTGAGAAAGCTCCGACGAAGAAAACAATCTCGTTGAATGATGAAGTTATCGTCTCCAAGCTACTAAAATATCCCGTTCGAGGGCTCGTGTTCGAGGGCGAAAACACTCTTCAAGCGTTATCGAACGCGGTGTCTGATGCCTGCATATGCCTTCAAGAAAACAACATTCCTTACAATTGCTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGTGGAGCTCCTCGACACCCAAGTGAACCCGGCCGTTTGGGAGATAAGTGGGCACATGGTGTTGAAGAGGAAGAAGGATTACGACGAGGCGTCGGAGGAAAACGCCTGGAGGCTCCTTGCTGAGGTATCCCTATCAGAAGAGAGGTTTCAAGAAGTGTGTGCTCTAATCTTTGAAGTCCTCGACACAGTTGATGTTGAAGGTAGCCCGAGCGTGGCTGAGGAGCACGGTGATGCTTGTCTCGAGGAAGTTGAAGCTAGAAACAACGCCTCCCGACCACTCCGGGTGACTGGGACGTGGGAATGCTTAGTTTAG

Protein sequence

MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCCEYENTAPPFAFLDSLLLGERPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACICLQENNIPYNCYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEVEARNNASRPLRVTGTWECLV

Homology

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BLAST of Carg12835 vs. NCBI nr
Match: KAG7024934.1 (GDP-L-galactose phosphorylase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 782.3 bits (2019), Expect = 1.9e-222
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGERPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNT 120
           EYENTAPPFAFLDSLLLGERPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNT
Sbjct: 61  EYENTAPPFAFLDSLLLGERPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNT 120

Query: 121 QLIPNAPLDVENSPSMVAINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGN 180
           QLIPNAPLDVENSPSMVAINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGN
Sbjct: 121 QLIPNAPLDVENSPSMVAINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGN 180

Query: 181 TYFRLGYNSLGAFATINHLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGL 240
           TYFRLGYNSLGAFATINHLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGL
Sbjct: 181 TYFRLGYNSLGAFATINHLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGL 240

Query: 241 VFEGENTLQALSNAVSDACICLQENNIPYNCYAEKQALGEVSVELLDTQVNPAVWEISGH 300
           VFEGENTLQALSNAVSDACICLQENNIPYNCYAEKQALGEVSVELLDTQVNPAVWEISGH
Sbjct: 241 VFEGENTLQALSNAVSDACICLQENNIPYNCYAEKQALGEVSVELLDTQVNPAVWEISGH 300

Query: 301 MVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDA 360
           MVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDA
Sbjct: 301 MVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDA 360

Query: 361 CLEEVEARNNASRPLRVTGTWECLV 386
           CLEEVEARNNASRPLRVTGTWECLV
Sbjct: 361 CLEEVEARNNASRPLRVTGTWECLV 385

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BLAST of Carg12835 vs. NCBI nr
Match: KAG6592057.1 (GDP-L-galactose phosphorylase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751.1 bits (1938), Expect = 4.6e-213
Identity = 384/440 (87.27%), Postives = 384/440 (87.27%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTAPPFAFLDSLLLGE                                        R
Sbjct: 61  EYENTAPPFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV
Sbjct: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 420

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BLAST of Carg12835 vs. NCBI nr
Match: XP_022976659.1 (GDP-L-galactose phosphorylase 1-like [Cucurbita maxima])

HSP 1 Score: 737.3 bits (1902), Expect = 6.9e-209
Identity = 376/440 (85.45%), Postives = 381/440 (86.59%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSN+QKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNYQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTAPPFAFLDSLLLGE                                        R
Sbjct: 61  EYENTAPPFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNT LIPNAPLDVENSPSMVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTLLIPNAPLDVENSPSMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVS+LLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSELLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLL EVSLSEERFQEVCALIFEVLDTVDVEGSPSVA+EH DACLEEV
Sbjct: 361 KKDYDEASEENAWRLLVEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAKEHSDACLEEV 420

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BLAST of Carg12835 vs. NCBI nr
Match: XP_023534897.1 (GDP-L-galactose phosphorylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 736.1 bits (1899), Expect = 1.5e-208
Identity = 377/440 (85.68%), Postives = 382/440 (86.82%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTAPPFAFLDSLLLGE                                        R
Sbjct: 61  EYENTAPPFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGN QLIPNAPLDVENSP+MVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNAQLIPNAPLDVENSPNMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVS+LLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSELLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQE+NIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQEHNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVA+EHGDACLEEV
Sbjct: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAKEHGDACLEEV 420

[Close]

BLAST of Carg12835 vs. NCBI nr
Match: XP_022936265.1 (GDP-L-galactose phosphorylase 1-like [Cucurbita moschata])

HSP 1 Score: 735.7 bits (1898), Expect = 2.0e-208
Identity = 379/440 (86.14%), Postives = 381/440 (86.59%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSN+QKDEAEEVAPRAGCGRNCLNECC EGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNYQKDEAEEVAPRAGCGRNCLNECCCEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTA PFAFLDSLLLGE                                        R
Sbjct: 61  EYENTA-PFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQ IDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQEIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVS+LLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSELLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV
Sbjct: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 420

[Close]

BLAST of Carg12835 vs. ExPASy Swiss-Prot
Match: Q8RWE8 (GDP-L-galactose phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=VTC2 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 3.2e-132
Identity = 253/442 (57.24%), Postives = 296/442 (66.97%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           ML+IKRVPTVVSN+QKD+  E     GCGRNCL  CC  GA++PLYA K L K  + L  
Sbjct: 1   MLKIKRVPTVVSNYQKDDGAE--DPVGCGRNCLGACCLNGARLPLYACKNLVKSGEKLVI 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
            +E   PP AFL+SL+LGE                                        R
Sbjct: 61  SHEAIEPPVAFLESLVLGEWEDRFQRGLFRYDVTACETKVIPGKYGFVAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQ FDG+KFNFTKVGQEE+LF+FEA E+   Q  P  P+D ENSPS+VAIN
Sbjct: 121 PTEFRVDKVLQSFDGSKFNFTKVGQEELLFQFEAGEDAQVQFFPCMPIDPENSPSVVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPR+ + LPQRID +S LLA+HMAAEA N YFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRVLDCLPQRIDHKSLLLAVHMAAEAANPYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLA+PFP+EKAPTKK  +    V +S+LL YPVR L+FEG +++Q LS+ VSD C+
Sbjct: 241 FQAYYLAMPFPLEKAPTKKITTTVSGVKISELLSYPVRSLLFEGGSSMQELSDTVSDCCV 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQ NNIP+N               CYAEKQALGEVS E+L+TQVNPAVWEISGHMVLKR
Sbjct: 301 CLQNNNIPFNILISDCGRQIFLMPQCYAEKQALGEVSPEVLETQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           K+DY+ ASE+NAWRLLAE SLSEERF+EV AL FE +   + E        H       V
Sbjct: 361 KEDYEGASEDNAWRLLAEASLSEERFKEVTALAFEAIGCSNQEEDLEGTIVHQQNSSGNV 420

[Close]

BLAST of Carg12835 vs. ExPASy Swiss-Prot
Match: Q9FLP9 (GDP-L-galactose phosphorylase 2 OS=Arabidopsis thaliana OX=3702 GN=VTC5 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.0e-125
Identity = 249/430 (57.91%), Postives = 292/430 (67.91%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           +L+IKRVPTVVSN+QKDE  E     GCGRNCL++CC  GA++PLY  K L+K S G   
Sbjct: 2   LLKIKRVPTVVSNYQKDETVE---EGGCGRNCLSKCCINGARLPLYTCKNLDK-SVG--- 61

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
             ENT  P  FL+SL++GE                                        R
Sbjct: 62  --ENTESPVTFLESLVIGEWEDRFQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 121

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANEN---GNTQLIPNAPLDVENSPSMV 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEE+LF+F+A+ N      Q + + PLD +NSPS+V
Sbjct: 122 PTEFRVDKVLQPFDGNKFNFTKVGQEELLFQFKASTNDDDSEIQFLASMPLDADNSPSVV 181

Query: 181 AINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATIN 240
           AINVSPIEYGHVLLIPR+ + LPQRID +S LLAL MAAEA N YFRLGYNSLGAFATIN
Sbjct: 182 AINVSPIEYGHVLLIPRVLDCLPQRIDHKSLLLALQMAAEADNPYFRLGYNSLGAFATIN 241

Query: 241 HLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSD 300
           HLHFQAYYLA+ FPIEKA + K  + N+ V +SKLL YPVRGL+ EG NT++ L++ VSD
Sbjct: 242 HLHFQAYYLAMQFPIEKASSLKITTTNNGVKISKLLNYPVRGLLVEGGNTIKDLADTVSD 301

Query: 301 ACICLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMV 360
           A +CLQ NNIP+N               CYAEKQALGEVS  LLDTQVNPAVWE+SGHMV
Sbjct: 302 ASVCLQNNNIPFNILISDSGKRIFLLPQCYAEKQALGEVSSTLLDTQVNPAVWEMSGHMV 361

Query: 361 LKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACL 373
           LKRK+DY+ ASEE AWRLLAEVSLSEERF+EV  +IF+ +         S  EE  +  L
Sbjct: 362 LKRKEDYEGASEEKAWRLLAEVSLSEERFREVNTMIFDAIGF-------SSHEEEEEEEL 415

[Close]

BLAST of Carg12835 vs. ExPASy Swiss-Prot
Match: Q3TLS3 (GDP-D-glucose phosphorylase 1 OS=Mus musculus OX=10090 GN=Gdpgp1 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 2.0e-25
Identity = 92/293 (31.40%), Postives = 137/293 (46.76%), Query Frame = 0


Query: 80  RPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAI 139
           RP   R   V Q FD  +FNF K+   E+LFR +    G     P  P   +    +V I
Sbjct: 108 RPQNIR--SVRQEFDPEQFNFNKIRPGEVLFRMQREPKG-----PATP--KQEDDVLVVI 167

Query: 140 NVSPIEYGHVLLIPRIFERLPQ-------RIDRESFLLALHMAAEAGNTYFRLGYNSLGA 199
           NVSP+E+GHVLL+P   + LPQ       R+  E+ LL+LH         FR+G+NSLG 
Sbjct: 168 NVSPLEWGHVLLVPAPAQGLPQRLLPGVLRVGLEAVLLSLHPG-------FRVGFNSLGG 227

Query: 200 FATINHLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGEN---TLQ 259
            A++NHLH   YYLA P P+E AP   +  L+ +  +  L   P  G +F        L+
Sbjct: 228 LASVNHLHLHCYYLAHPLPVEGAP---STPLDPKGCIHLLQALPAPGFLFYTSGPGPDLE 287

Query: 260 ALSNAVSDACICLQENNIPYNCYAEKQA----------LGEVSVELLDTQ---------- 319
            L + V  A   L +  I +N +  + A          L  + V L   +          
Sbjct: 288 VLISRVCRATDYLSDREIAHNLFVTRGAPPGPTSSTSDLSGIRVILWARKSSFGIKESGA 347

Query: 320 VNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVL 343
            N A+ E++GH+ +K  +D+   +E  A  L+ +  L E +  EV A +  ++
Sbjct: 348 FNVALCELAGHLPVKTSQDFSSLTEAAAVALIQDCLLPETQAGEVRAALVALM 381

[Close]

BLAST of Carg12835 vs. ExPASy Swiss-Prot
Match: Q6ZNW5 (GDP-D-glucose phosphorylase 1 OS=Homo sapiens OX=9606 GN=GDPGP1 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 8.4e-24
Identity = 85/287 (29.62%), Postives = 135/287 (47.04%), Query Frame = 0


Query: 86  VDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAINVSPIE 145
           +  V Q FD  +FNF K+   E+LFR            P+ P  +     +V INVSP+E
Sbjct: 112 IKSVRQAFDPVQFNFNKIRPGEVLFRLHRE--------PDLPGTLLQEDILVVINVSPLE 171

Query: 146 YGHVLLIPRIFERLPQRI-------DRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 205
           +GHVLL+P    +LPQR+         E+ LL+LH         FR+G+NSLG  A++NH
Sbjct: 172 WGHVLLVPEPARQLPQRLLPGALRAGIEAVLLSLHPG-------FRVGFNSLGGLASVNH 231

Query: 206 LHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGEN---TLQALSNAV 265
           LH   YYLA   P+E+AP++    L+    +  L   P  G +F        L++L + V
Sbjct: 232 LHLHGYYLAHRLPVEQAPSE---PLDPGGHLHLLQDLPAPGFLFYTRGPGPDLESLISRV 291

Query: 266 SDACICLQENNIPYNCYAEKQA-LGEVSVELLDTQVNPAVW------------------- 325
             A   L ++ I +N +  + A  G+ S     T V   +W                   
Sbjct: 292 CRATDYLTDHEIAHNLFVTRGAPPGKTSPSSALTGVRVILWARKSSFGIKDGEAFNVALC 351

Query: 326 EISGHMVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVL 343
           E++GH+ +K  +D+   +E  A  L+ +  L   + ++V A +  ++
Sbjct: 352 ELAGHLPVKTSQDFSSLTEAAAVALIQDCRLPPSQAEDVQAALVALM 380

[Close]

BLAST of Carg12835 vs. ExPASy Swiss-Prot
Match: Q8HXE4 (GDP-D-glucose phosphorylase 1 OS=Macaca fascicularis OX=9541 GN=GDPGP1 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.2e-23
Identity = 85/287 (29.62%), Postives = 133/287 (46.34%), Query Frame = 0


Query: 86  VDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAINVSPIE 145
           +  V Q FD  +FNF K+   E+L+R            P+ P  +     +V INVSP+E
Sbjct: 112 IKSVRQAFDPEQFNFNKIQPGEVLYRLHRE--------PDLPGTLLQEDILVVINVSPLE 171

Query: 146 YGHVLLIPRIFERLPQRI-------DRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 205
           +GHVLL+P     LPQR+         E+ LL+LH         FR+G+NSLG  A++NH
Sbjct: 172 WGHVLLVPEPARGLPQRLLPGALRAGIEAVLLSLHPG-------FRVGFNSLGGLASVNH 231

Query: 206 LHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGEN---TLQALSNAV 265
           LH   YYLA   P+E+AP++    L+    +  L   P  G +F        L++L   V
Sbjct: 232 LHLHGYYLAHRLPVEQAPSE---PLDPGGHLHLLQGLPAPGFLFYTRGPGLDLESLICRV 291

Query: 266 SDACICLQENNIPYNCYAEKQA-LGEVSVELLDTQVNPAVW------------------- 325
             A   L ++ I +N +  + A  G+ S     T V   +W                   
Sbjct: 292 CRATDYLTDHEIAHNLFVTRGAPPGKTSPSSALTGVRVILWARKSCFGIKDGEAFNVALC 351

Query: 326 EISGHMVLKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVL 343
           E++GH+ +K  +D+   +E  A  L+ +  L   + +EV A +  ++
Sbjct: 352 ELAGHLPVKTSQDFSSLTEAAAVALIQDCRLPPSQAEEVQAALVALM 380

[Close]

BLAST of Carg12835 vs. ExPASy TrEMBL
Match: A0A6J1IP60 (GDP-L-galactose phosphorylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476994 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.4e-209
Identity = 376/440 (85.45%), Postives = 381/440 (86.59%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSN+QKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNYQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTAPPFAFLDSLLLGE                                        R
Sbjct: 61  EYENTAPPFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNT LIPNAPLDVENSPSMVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTLLIPNAPLDVENSPSMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVS+LLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSELLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLL EVSLSEERFQEVCALIFEVLDTVDVEGSPSVA+EH DACLEEV
Sbjct: 361 KKDYDEASEENAWRLLVEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAKEHSDACLEEV 420

[Close]

BLAST of Carg12835 vs. ExPASy TrEMBL
Match: A0A6J1FD57 (GDP-L-galactose phosphorylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442936 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 9.8e-209
Identity = 379/440 (86.14%), Postives = 381/440 (86.59%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           MLRIKRVPTVVSN+QKDEAEEVAPRAGCGRNCLNECC EGAKIPLYAFKKLNKISKGLCC
Sbjct: 1   MLRIKRVPTVVSNYQKDEAEEVAPRAGCGRNCLNECCCEGAKIPLYAFKKLNKISKGLCC 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
           EYENTA PFAFLDSLLLGE                                        R
Sbjct: 61  EYENTA-PFAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN
Sbjct: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPRIFERLPQ IDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRIFERLPQEIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLAVPFPIEKAPTKKTISLNDEVIVS+LLKYPVRGLVFEGENTLQALSNAVSDACI
Sbjct: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSELLKYPVRGLVFEGENTLQALSNAVSDACI 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVLKR
Sbjct: 301 CLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV
Sbjct: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 420

[Close]

BLAST of Carg12835 vs. ExPASy TrEMBL
Match: A0A0A0K5F4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219200 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.0e-182
Identity = 334/445 (75.06%), Postives = 356/445 (80.00%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKI--SKGL 60
           MLRIKRVPTVVSN+Q+DE E V+   GCGRNCLN+CC EGAKIPLYAFKKLNKI  SKGL
Sbjct: 1   MLRIKRVPTVVSNYQEDETENVSRSTGCGRNCLNKCCIEGAKIPLYAFKKLNKISGSKGL 60

Query: 61  CCEYENTAPPFAFLDSLLLGE--------------------------------------- 120
           CCEYEN+ PP AFLDSLLLGE                                       
Sbjct: 61  CCEYENSVPPVAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLK 120

Query: 121 -RPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVA 180
            RPTEFRVDKVLQPFDGNKFNFTKVGQEE+LFRFEANENGNTQ IPN  +D+ENSPS+VA
Sbjct: 121 KRPTEFRVDKVLQPFDGNKFNFTKVGQEEVLFRFEANENGNTQFIPNDAIDLENSPSVVA 180

Query: 181 INVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 240
           INVSPIEYGHVLLIPRI + LPQRIDRESFLLALHMA EAGNTYFRLGYNSLGAFATINH
Sbjct: 181 INVSPIEYGHVLLIPRILDCLPQRIDRESFLLALHMATEAGNTYFRLGYNSLGAFATINH 240

Query: 241 LHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDA 300
           LHFQAYYL VPFPIEKAPTKK ++L D VI+S+LLKYPVRGLVFEG NTLQ+LSN+VSDA
Sbjct: 241 LHFQAYYLGVPFPIEKAPTKKIMTLKDGVIISELLKYPVRGLVFEGGNTLQSLSNSVSDA 300

Query: 301 CICLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVL 360
           CICLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVL
Sbjct: 301 CICLQENNIPYNILIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVL 360

Query: 361 KRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVD---VEGSPSVAEEHGDA 386
           KRKKDY+EASEENAWRLLA VSLSEERFQEVCALIFEV+DTVD    EGS S+ EEH D 
Sbjct: 361 KRKKDYEEASEENAWRLLAVVSLSEERFQEVCALIFEVIDTVDAENTEGSSSMPEEHDDG 420

[Close]

BLAST of Carg12835 vs. ExPASy TrEMBL
Match: A0A5D3DHL7 (GDP-L-galactose phosphorylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00510 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.0e-181
Identity = 334/452 (73.89%), Postives = 354/452 (78.32%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKI--SKGL 60
           MLRIKRVPTVVSN+Q+DE E V+ + GCGRNCLN+CC EGAKIPLYAFKKLNKI  SKGL
Sbjct: 86  MLRIKRVPTVVSNYQEDETENVSRKTGCGRNCLNKCCIEGAKIPLYAFKKLNKITGSKGL 145

Query: 61  CCEYENTAPPFAFLDSLLLGE--------------------------------------- 120
           CCEYEN+ PP AFLDSLLLGE                                       
Sbjct: 146 CCEYENSVPPVAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLK 205

Query: 121 -RPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVA 180
            RPTEFRVDKVLQPFDGNKFNFTKVGQEE+LFRFEANEN NTQ IPNA  DVENSPS+VA
Sbjct: 206 KRPTEFRVDKVLQPFDGNKFNFTKVGQEEVLFRFEANENENTQFIPNAAFDVENSPSVVA 265

Query: 181 INVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 240
           INVSPIEYGHVLLIPR+ E LPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH
Sbjct: 266 INVSPIEYGHVLLIPRVLECLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 325

Query: 241 LHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDA 300
           LHFQAYYL VPFPIEKAPTKK I+L D VI+S+LLKYPVRGL+FEG NTLQ+LSN+VSDA
Sbjct: 326 LHFQAYYLGVPFPIEKAPTKKIITLKDGVIISELLKYPVRGLIFEGGNTLQSLSNSVSDA 385

Query: 301 CICLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVL 360
           CICLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVL
Sbjct: 386 CICLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVL 445

Query: 361 KRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVA--------- 386
           KRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEV+DTVD E +   +         
Sbjct: 446 KRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVIDTVDAENTEDSSSMPEEHGDG 505

[Close]

BLAST of Carg12835 vs. ExPASy TrEMBL
Match: A0A1S3BIN8 (GDP-L-galactose phosphorylase 1 OS=Cucumis melo OX=3656 GN=LOC103490128 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.9e-181
Identity = 334/452 (73.89%), Postives = 354/452 (78.32%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKI--SKGL 60
           MLRIKRVPTVVSN+Q+DE E V+ R GCGRNCLN+CC EGAKIPLYAFKKLNKI  SKGL
Sbjct: 1   MLRIKRVPTVVSNYQEDETENVSRRTGCGRNCLNKCCIEGAKIPLYAFKKLNKITGSKGL 60

Query: 61  CCEYENTAPPFAFLDSLLLGE--------------------------------------- 120
           CCEYEN+ PP AFLDSLLLGE                                       
Sbjct: 61  CCEYENSVPPVAFLDSLLLGEWEDRMQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLK 120

Query: 121 -RPTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVA 180
            RPTEFRVDKVLQPFDGNKFNFTKVGQEE+LFRFEANEN NTQ IPNA  DVENSPS+VA
Sbjct: 121 KRPTEFRVDKVLQPFDGNKFNFTKVGQEEVLFRFEANENENTQFIPNAAFDVENSPSVVA 180

Query: 181 INVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 240
           INVSPIEYGHVLLIPR+ E LPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH
Sbjct: 181 INVSPIEYGHVLLIPRVLECLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINH 240

Query: 241 LHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDA 300
           LHFQAYYL VPFPIEKAPTKK I+L D VI+S+LLKYPVRGL+FEG NTLQ+LSN+VSDA
Sbjct: 241 LHFQAYYLGVPFPIEKAPTKKIITLKDGVIISELLKYPVRGLIFEGGNTLQSLSNSVSDA 300

Query: 301 CICLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVL 360
           CICLQENNIPYN               CYAEKQALGEVS ELLDTQVNPAVWEISGHMVL
Sbjct: 301 CICLQENNIPYNVLIADCGQRIFLLPQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVL 360

Query: 361 KRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVA--------- 386
           KRKKDY+EASEENAWRLLAEVSLSEERFQEVCALIFEV+DTVD E +   +         
Sbjct: 361 KRKKDYEEASEENAWRLLAEVSLSEERFQEVCALIFEVIDTVDAENTEDSSSMPEEHGDG 420

[Close]

BLAST of Carg12835 vs. TAIR 10
Match: AT4G26850.1 (mannose-1-phosphate guanylyltransferase (GDP)s;GDP-galactose:mannose-1-phosphate guanylyltransferases;GDP-galactose:glucose-1-phosphate guanylyltransferases;GDP-galactose:myoinositol-1-phosphate guanylyltransferases;glucose-1-phosphate guanylyltransferase )

HSP 1 Score: 473.0 bits (1216), Expect = 2.3e-133
Identity = 253/442 (57.24%), Postives = 296/442 (66.97%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           ML+IKRVPTVVSN+QKD+  E     GCGRNCL  CC  GA++PLYA K L K  + L  
Sbjct: 1   MLKIKRVPTVVSNYQKDDGAE--DPVGCGRNCLGACCLNGARLPLYACKNLVKSGEKLVI 60

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
            +E   PP AFL+SL+LGE                                        R
Sbjct: 61  SHEAIEPPVAFLESLVLGEWEDRFQRGLFRYDVTACETKVIPGKYGFVAQLNEGRHLKKR 120

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANENGNTQLIPNAPLDVENSPSMVAIN 180
           PTEFRVDKVLQ FDG+KFNFTKVGQEE+LF+FEA E+   Q  P  P+D ENSPS+VAIN
Sbjct: 121 PTEFRVDKVLQSFDGSKFNFTKVGQEELLFQFEAGEDAQVQFFPCMPIDPENSPSVVAIN 180

Query: 181 VSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATINHLH 240
           VSPIEYGHVLLIPR+ + LPQRID +S LLA+HMAAEA N YFRLGYNSLGAFATINHLH
Sbjct: 181 VSPIEYGHVLLIPRVLDCLPQRIDHKSLLLAVHMAAEAANPYFRLGYNSLGAFATINHLH 240

Query: 241 FQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSDACI 300
           FQAYYLA+PFP+EKAPTKK  +    V +S+LL YPVR L+FEG +++Q LS+ VSD C+
Sbjct: 241 FQAYYLAMPFPLEKAPTKKITTTVSGVKISELLSYPVRSLLFEGGSSMQELSDTVSDCCV 300

Query: 301 CLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMVLKR 360
           CLQ NNIP+N               CYAEKQALGEVS E+L+TQVNPAVWEISGHMVLKR
Sbjct: 301 CLQNNNIPFNILISDCGRQIFLMPQCYAEKQALGEVSPEVLETQVNPAVWEISGHMVLKR 360

Query: 361 KKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACLEEV 386
           K+DY+ ASE+NAWRLLAE SLSEERF+EV AL FE +   + E        H       V
Sbjct: 361 KEDYEGASEDNAWRLLAEASLSEERFKEVTALAFEAIGCSNQEEDLEGTIVHQQNSSGNV 420

[Close]

BLAST of Carg12835 vs. TAIR 10
Match: AT5G55120.1 (galactose-1-phosphate guanylyltransferase (GDP)s;GDP-D-glucose phosphorylases;quercetin 4'-O-glucosyltransferases )

HSP 1 Score: 451.4 bits (1160), Expect = 7.1e-127
Identity = 249/430 (57.91%), Postives = 292/430 (67.91%), Query Frame = 0


Query: 1   MLRIKRVPTVVSNFQKDEAEEVAPRAGCGRNCLNECCYEGAKIPLYAFKKLNKISKGLCC 60
           +L+IKRVPTVVSN+QKDE  E     GCGRNCL++CC  GA++PLY  K L+K S G   
Sbjct: 2   LLKIKRVPTVVSNYQKDETVE---EGGCGRNCLSKCCINGARLPLYTCKNLDK-SVG--- 61

Query: 61  EYENTAPPFAFLDSLLLGE----------------------------------------R 120
             ENT  P  FL+SL++GE                                        R
Sbjct: 62  --ENTESPVTFLESLVIGEWEDRFQRGLFRYDVTACETKVIPGKYGFIAQLNEGRHLKKR 121

Query: 121 PTEFRVDKVLQPFDGNKFNFTKVGQEEILFRFEANEN---GNTQLIPNAPLDVENSPSMV 180
           PTEFRVDKVLQPFDGNKFNFTKVGQEE+LF+F+A+ N      Q + + PLD +NSPS+V
Sbjct: 122 PTEFRVDKVLQPFDGNKFNFTKVGQEELLFQFKASTNDDDSEIQFLASMPLDADNSPSVV 181

Query: 181 AINVSPIEYGHVLLIPRIFERLPQRIDRESFLLALHMAAEAGNTYFRLGYNSLGAFATIN 240
           AINVSPIEYGHVLLIPR+ + LPQRID +S LLAL MAAEA N YFRLGYNSLGAFATIN
Sbjct: 182 AINVSPIEYGHVLLIPRVLDCLPQRIDHKSLLLALQMAAEADNPYFRLGYNSLGAFATIN 241

Query: 241 HLHFQAYYLAVPFPIEKAPTKKTISLNDEVIVSKLLKYPVRGLVFEGENTLQALSNAVSD 300
           HLHFQAYYLA+ FPIEKA + K  + N+ V +SKLL YPVRGL+ EG NT++ L++ VSD
Sbjct: 242 HLHFQAYYLAMQFPIEKASSLKITTTNNGVKISKLLNYPVRGLLVEGGNTIKDLADTVSD 301

Query: 301 ACICLQENNIPYN---------------CYAEKQALGEVSVELLDTQVNPAVWEISGHMV 360
           A +CLQ NNIP+N               CYAEKQALGEVS  LLDTQVNPAVWE+SGHMV
Sbjct: 302 ASVCLQNNNIPFNILISDSGKRIFLLPQCYAEKQALGEVSSTLLDTQVNPAVWEMSGHMV 361

Query: 361 LKRKKDYDEASEENAWRLLAEVSLSEERFQEVCALIFEVLDTVDVEGSPSVAEEHGDACL 373
           LKRK+DY+ ASEE AWRLLAEVSLSEERF+EV  +IF+ +         S  EE  +  L
Sbjct: 362 LKRKEDYEGASEEKAWRLLAEVSLSEERFREVNTMIFDAIGF-------SSHEEEEEEEL 415

The following BLAST results are available for this feature:

BLAST of Carg12835 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024934.1	1.9e-222	100.00	GDP-L-galactose phosphorylase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6592057.1	4.6e-213	87.27	GDP-L-galactose phosphorylase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022976659.1	6.9e-209	85.45	GDP-L-galactose phosphorylase 1-like [Cucurbita maxima]	[more]
XP_023534897.1	1.5e-208	85.68	GDP-L-galactose phosphorylase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022936265.1	2.0e-208	86.14	GDP-L-galactose phosphorylase 1-like [Cucurbita moschata]	[more]

BLAST of Carg12835 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWE8	3.2e-132	57.24	GDP-L-galactose phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=VTC2 PE=1 SV=...	[more]
Q9FLP9	1.0e-125	57.91	GDP-L-galactose phosphorylase 2 OS=Arabidopsis thaliana OX=3702 GN=VTC5 PE=1 SV=...	[more]
Q3TLS3	2.0e-25	31.40	GDP-D-glucose phosphorylase 1 OS=Mus musculus OX=10090 GN=Gdpgp1 PE=1 SV=2	[more]
Q6ZNW5	8.4e-24	29.62	GDP-D-glucose phosphorylase 1 OS=Homo sapiens OX=9606 GN=GDPGP1 PE=1 SV=2	[more]
Q8HXE4	3.2e-23	29.62	GDP-D-glucose phosphorylase 1 OS=Macaca fascicularis OX=9541 GN=GDPGP1 PE=2 SV=1	[more]

BLAST of Carg12835 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IP60	3.4e-209	85.45	GDP-L-galactose phosphorylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476994...	[more]
A0A6J1FD57	9.8e-209	86.14	GDP-L-galactose phosphorylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114429...	[more]
A0A0A0K5F4	2.0e-182	75.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219200 PE=3 SV=1	[more]
A0A5D3DHL7	3.0e-181	73.89	GDP-L-galactose phosphorylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BIN8	3.9e-181	73.89	GDP-L-galactose phosphorylase 1 OS=Cucumis melo OX=3656 GN=LOC103490128 PE=3 SV=...	[more]

BLAST of Carg12835 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26850.1	2.3e-133	57.24	mannose-1-phosphate guanylyltransferase (GDP)s;GDP-galactose:mannose-1-phosphate...	[more]
AT5G55120.1	7.1e-127	57.91	galactose-1-phosphate guanylyltransferase (GDP)s;GDP-D-glucose phosphorylases;qu...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026506	GDP-L-galactose/GDP-D-glucose phosphorylase	PANTHER	PTHR20884	GDP-D-GLUCOSE PHOSPHORYLASE 1	coord: 79..385 coord: 1..79
None	No IPR available	PANTHER	PTHR20884:SF15	GDP-L-GALACTOSE PHOSPHORYLASE	coord: 79..385 coord: 1..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12835-RA	Carg12835-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0080048	GDP-D-glucose phosphorylase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0016779	nucleotidyltransferase activity