Carg12832 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12832
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description1,4-alpha-glucan branching enzyme
LocationCarg_Chr09: 5378737 .. 5395308 (+)
RNA-Seq ExpressionCarg12832
SyntenyCarg12832
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACCAGCACAGTTTCCCCGCCATGAACGCCCTTTCAAGTTCACCTTCCCTCAAATTCCCTCCTTACAAGACCAATCATCTCCTTCTACTACCTCACTCCCGCTCCACGCTTCAATCCATCGCTCTGTCTCGGAAACTAAAATTGAAGATAATTTGTTCAGCATCCGAACATTCACAACATGCTGAACCAGCGAAGTACCAGCACTCTAAGAAGACGAAAAGCGTCTCGGATGGCGAGAAAGGGGTTGATCCTGTTGGATTTCTCACTAAACTCGGCATTTCTCACAAGCAATTTGCTCATTTCCTCCGCGAAAGGTAGTTTTCTTGGTTTTGTTTCGTCTCATGACAATGAATTTGGAAAATGGAAGCTGAATGAGTGTGGAGTGATCATCCATGGAAATTTTGTTTCGAGAATTGAGAAATGTTTCGTTTTATTATAATAATATCGTAAACATGGCTATCTTATTTACTTGTAGATATAAATCGTTGAAGGACCTCAAGGATGAAATTTACGATCGTCATGCGAATCTCCAGGACCTGTCTTCTGGGTTAGTTGAGTTTTTCATTTTCATTTTCATCATTATCTATTACTCTTTCCTTTTTAGTTTAATTTTGTGCTTGGAACTTGGCACAATTGTTATTCCCATTCTCTTTCAGCACGTTCTTGGTTTTTTTGCACACATGAATCTAACGCTTTTGATATTTTGGATATAGATTTATGTTTTTAGGTATGCATCGCCATGTGGAACATCGTGTTGATTTTATGGAATGGGCTCCAGGTTTGGATTCCATGCTTCTGCTTTTCCTGCTCTAGATAATATATGATTGTGGAATATATGTAATGAACATCATGTTGTACATGCAGAGCCATTCAGATTATGTGGGTGACCTGAGTCTTGATTTAACTCAGCTGGTTGCGTTTTGTAATTTGGAGAATTCTCTTGCTAATCAATTTTTTTTGTACCATATCAATGTCAGAACATCTGCCCTCTGTTGTGTTCTAGTGAATCTTGGGTGGGAACTTGTAACAGCCTAAACCCACCGCTTGTAGATATTGTCCTCTTTGTGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGCGTTTGCTAGAGAGAGGTTTCTACACCCTTGTAAAGAATGCTTTGTTCCCTTCTTCAATTGATGTGGAATCTCACAATCCACCACCCTTCAGGGTCCAGCGTCCTTGTTGGCACTCGTTACTCTCTCTAATCGATGTGAGATCTCACAATCCACCATTCTTTGGGGCCCAACATCCTTGCTGGCAAACCCCCCAGTGTCCACCTTTTCCCTTACAAGTGTATTTATGTGATCACCAAGGTGATGAGTGAGGCCATTCCCCAACTTTTATTTTCAGATTCTAGTTGGGTGTAATCCAGACTTCTGGAGTTTCTTGATTCCTAGTGCATCCATAGCAGCCTTTTAATTTCTGTTTTCAGATTTCCACTGGACATCCTTCTCATGGTCAAATCTAAAAATATTTGATTCAATATGACTATAATTTTGATGGAAAGTCTGGAGATGTGAATGGAGTTTTGGACTCAGATGGTTTGTTAGTCTCTTCTTGGGCCTCCTTGTCTAATTTATTTTCTGCTCTCTTATCTTAGTTATGCTAACTCGAGTGCATTGATGCTTCTATATTTGTTTGTTATGCTGAAGTCTGGTTAAGTTCTTTGTTGGTTGTTAATCTTCATTTTGCTGCATACCTTGCCTTTGTGGAGATCACCGGTTTTTCTTTTTGCTTGAGAAATACTTCGTTGGACAAGTTCCTCTTTCTGCTCTATTTTATTTCTTCAACCTAACTTAGTTGATCTTGATCTTCAACTTAAACATTGCTTTTATCTTTTCTCTAGGTTGTGAAAGATACAAAATAACAAAAAACACTATTGAGCTTGAAGTTTCAATTTTAGAAGTGCCAACCATCTTTTTGAAGTCTTAGGGAAACCTAATTTCTATTGGTTTTTGCTTTGCTTTACCACTCATTTTAATAACTAGTTAACTTTTCTGTTTGTAGGTGCTCGCTATTGTGCAGTAATTGGTGACTTCAATGATTGGTCACCCAGAGAAAACGTTGCCAGAGAGGGGCATCTTGGCCATGATGATTATGGTTACTGGTTTATCATCCTCGAAGATAGGTTGAAGGAAGGAGAGAAACCAGAGGAACTCTATTTTCAACAGTATAATTACGTGGATGATTATGATAAAGGTGACAGTGGAATCAGCATTGACGAGATCTTCAAAAGAGCGAATGATGAATATTGGGAACCTGGAGAGGACAGGTTCATCAAAAACCGTTTTGAAGTTCCAGCTAAGCTCTATGAAGAATTATTTGGCCCCAATGGTCCTCAAACATTAGAAGAGCTTGAAGAAATACCAGATGCAGAAACAAGATACAAGGCATGGAAAGAACAACATAAAGATGATCCATCTAGTGTTTCATATGACGTGATTGATAATGGGAAGGAGTATGATATTTTTAACGTGGTGATTGATCCCGTAACAAGGGAGAAATTTAAGGTTAAGAAACCTCCTATACCTTACTGGTTAGAAACACGTAAGGGAAGGAAAGCTTGGTTAAAAAAGTATAATCCCACTATTCCACATGGAAGCAAATACAGGGTATATTTCAACACTCCTGATGGGCCATTGGAAAGAATTCCTGCTTGGGCTACTTATGTGCAGCCAGGTAATGAAGGACAAATAATTTCTAGGATTTATCTAAGATATTTTTCGTTACTATCTGTGCTATACTATCCCGAGGCAGTTTTTAGAAAGTTTTGATGATTTTTCCATGTTAACTGAAGTTCAGTAATTTTATCTTAACTGGTGTATTGGTTAAAAAATTCAGATGCTGAAGGGAAGCAAGGTTTTGCAATCCATTGGGAACCACCACCAGAATATGCATATAACTGGAAAAACACCAGGCCTAATGTACCAAAGGCCTTGAAAATATATGAATGTCATGTGGGAATTAGTGGACCAGAGCCGAAAGTTTCTTCTTTCGACTACTTTAGAGAGAAGGTGAGTCTCCTCTGCTTCAACTATATGCACGTGCATTATGATAACCAATATGTTTTTCATTGTAAGAAGCCATACTTTTATGGAGAAAAATGAAAGAATACAAGAGCATTGAAAAAAGGCCCAACAAAAGGAATCTGTCCCTAACTACAAAAAAGACGCCAATCCAGTAGAACAACCAAGCTCATAATTACAAAATGCCCCAATGACCGGTAGATGGCGCCTATAAGGAAGTATTAAACCTGGCTACCTCCCAAAGCTCCTCATATGATCTCTTGGCCTCTCTAAAAATTCTATTGTTCCTCTCAAGCCCAATACCTCACAATACTTAACAGAACTTCCAAGGATCCAGCATGCCACAAAACCTTACTCTTTTCCGCCTTAGAAGGAGAATTCGGGAGCCCCTTCTCCATCAACTATCTACCAATATGTTGATGCAATTGATATTTCAAAAAAATTGTGAAGATGATTTAGAAATATTAGATGTTTGAATATACCACAAAAAAATCAAATGCTCATTTAATAAAAGAACACTGGATTCGTGTACCATTAGATATACTAATGCTTCTTTTACATGTGAAACACACAGGAATAAACAAATATTATGTTCGTAGACATTAAAGTTTGTTAAAAACACATGGAAATTTTTTATTAGTGCATTTAAGCTTCTGAAAGTGTCTAGATTGTGTGATTATCGTGTAAGGAATAGCTAATTAAACTACTTGACTTGTGACAGTTGACGATCTTTTGTTTGAAGCAGGAACTTTTAAAGATATCAAATTTTCAGTTTGAAAATAATCAATAATGTATAAAATGCATTGATTTAAGAGATTATATAATCTCAAATATGTGAGGGTGTGCGTGTGTGTGAGGCTATTCATTCCATGAAAAAAATATAATTTGCACAAAAGTCTTGGAGTTATAAGAAAAGGTCTCCAACTGGAAACATTACGGATTATATTTAATACAGCATCAGGTTTATATAGCTTGTAGGGTTAGCAAATTTGTAACTCAGAATCAGATTAAACATAAGCTCCGCTTGTTAACAATGAGTTATACAAACCTAAGACGCTCCCTTATGTTTGTCATATTTCTTTAATTGTTTTGTAAGTTTTCATATAAAAAGACATTGTCAACAGCAAATCATCTAACATTTTAACTTATACTTCGGCTTTCATTTCATTGTAACATTACATGACGTTGGTTCATTTTCACTTTCAACTACGTTTTATCTCGCAGGTTCTTCCACATGTGAAGGAAGCTGGATATAACGCAATCCAACTGTTTGGAGTGGTTGAACATAAAGATTACTTTACACTTGGCTATAGGGTAAGATAGTTATTCTTGCATCTCTGCTGTTATCGAACAATTTAGGGCTTCTACGTTTTTGAACATTCATATTGGCGACCTTTTGTTTTGGGTATTGGTCCGTAGAAGTTATTGGGCTTTGGATATGTTTGGATTTTTTTGCCATCGTCTTTGAAGTACGAATAAAGAATTTTTTTTTCCTGCATATGAAGGGCCGAGTGAGTATTATGCTGCCAATATGATAATGCTATGTGAGATGGACAGAAAGGCAAAGAATTAGGTGGTCGTAGAGGAGGGACAAAGAGAGTGATCAAGAGAGAGATGAAAAGGCAGACAAAGAGTGTGCTAGTTTTTTAATTTCTGATCTGTGTGCTCTCTGTGAGATCTTTTGCACATTTAAGTTTGTCCTCATTAATGCCGATATAGTGTACTTTTGTTGACCTTGAGTAGGTGGCTCTACATTGCTGTAGTAAATCATTCCATTATGGTAATTGGCTACTAGTTCGAAATCATGTTTGATTAATCAATGAAGGATTAATCAAACAAAGAAAAGACGATAAAGAAGCTTAAGCTCTAGCAGCTCCCTTTTTATTTTTGTTAAAAAGATGCTTAAATAAATTATGCTTACACAAATTTAGGGGTGAATTGTACTTTAAGATGTCTACAGATCCTGTTTAACTGACTGGTAGACTATGATTGCAAAATTGGTTCTTTTTTCTATTTTTCTGTTGTGAGGGTGGGTTGAGGGGGAGCAACAGGGGATCAGGCTTTAGCATTGTAAATGATTAAGCATCTTTATGCAGGTTACTAACTTCTTTGCTGTCAGCAGTAGATATGGGACCCCTGAGGACTTCAAGCGCTTGGTTGATGAAGCTCATGGTTATAATTCATTATCTATTACAATTCTTTTCGTTTACTCACGTTAAATATTTTGTTGGTTCTATTTTCTCTTCATCTTGTGTTAAATAAAAGGGTTATCATTTTCTTTGCAATTGAATTTGTAAGTGCTTCTTAGCTTACTAGACAGGCTAGATCACGTCGACAACTGAAGTGTTTTCAGATGCTGGTCAAGAGTCCCCTATAAAGCGAAAAAATTTCCATATCCCATATGAACAAAATGTCCTATCTGCTGTCGAGAGTTTTATATGATATGGAAGAATTTGCATGTACCCAAAACTGGTGTTTTTTGGCTGCTGGTTAAGAGTCCTATACAATGTGGAAAGATATGGTGTGGTCAAGTAAAACTGCCTTTATGATTTAGATTGCAGTCTGGTTAAGTTACTGCATTCTTGAACGTTCATGCAAGATCACTGTCTTTGTTTCTTTTCTTTTTTCCTTTTATTATTATGATTATTAATTTTTTAAAAAATTTAGAATGTGAATTTTGCATGCATTCCATTCATTTTTTTTTTTTTGTTATTTTAATTGAAGAGAAAGTGAAAGCTTACGTACTTTTAGTTTGCACTTTTATTTATTTTTGGCACAGGCCTTGGACTTTTTGTCTTCTTGGATATTGTTCATTCATATGCTGCTGCTGATGAGATGGTCGGACTGTCATCATTTGATGGATCAAATGACTGCTACTTTCATACTGGTATTTTTTGAGCCTTGATTACTCACTTTATTAGCTAGTAGGCCTTAGCCGTCTGTCAGTCTACATTGCCTCTTGATGCCAACGTTGCTTTTAAGTGGCTGGGCCTGTGTTCTAATTCATGGTTGTTAATGGAAAAAATTATTATTAATATTTTGACTTGATTCTTTTAACTGTATCTTGTTTCTTTTGTGATTGGATTTTCTTATTTATATATATTTTTCTTTTCTGTTCCACCCCCAAGACCTTGTACAAGGAGGCATTCAAAGATGTTGCAAAATTGAAGTTTGGCATAAAATGTTAGATTATTGATGTCACTATTTATTTGAAACCTACCATTATCCACCAAATTAATTGTGCTCTACCTCCTACTAGGAGCATTTGCTGCTTCTTCAATTGCGGGCTTCCAACTAGTTTGAATTTGTAGGAAATTGGAAAGTTTACAAGATCCTAAATTCAGCACACATTAGCAACAACAATTTTCTTAATAAAAGCAAAGAAGGAAATTATAGAAACGATCGTATAGGTTCACCCTGGTGTATTGTTTCATAAGGATGAAGGTTAACTTGAGATGAAGAATATAAAATACATGCCTTCACAAGAATATGTCTCTCTGCAAAAATCATTCGAAGGAGCTCTAAAGTTCTTATAAAATGCTCACAACTAGCCTTTCTGACCTTTTCCAACACTCTTTATGTTTCCCCAACAAGTTGTGCGCCGGAAGTGGAACGAATGTACTCACAACACCAATGAACAGAATCACACAAAGAGACACCAAGATATTTACGTGGTTCGGAGAGAAGAAGTTCCCCACCTCCACGGGTAGCAATCAATCACTAAAAACCCAAGTGTTACAAGTGAATACCTCACTATCACACTACAAAGTCTCTCACCAATGTGTCACCCAACAAATACAATGTGACACCAAAAACTCTCCCAACTCCCGAATCCCACTTGTTGTCAATCGATATCCAGAATGGAGGGAAAACTCTCTCATTATATAGCGTCACAAATCAAATCTCTGACTGAGACTAAATCTAATTCAAATAACCAATCGAATATAATTCAAATCCAAAAACCAAATCAAATCTAATCCAAAAAACCAAATCAAATCTTATGACAATTTACAACAATCTCCACCTTGTCATAAGATCTCTAAGTTGATAACTGAGTTGGTTGCCACCACCTCAAGCCTCAATATGTATGTTGGGAACTCCATCGATCATAGAGAAAGCATATGCTATTGTATCATCAAACCTTATAGGTTTTCGAATTACCCTTCGTGGTCTATTCTTGGCGATAGACTCAGGTTCCTCCACTTTTTCATTTACATGGCTTTGCTCCTCCTGTTCTAAGACATCAGAGTTATTATCTATCTGATCAATAGGTTCTTCAATAGGAGCAACCATATCAGGAGAGAACACCACCTTCTCCACTTGCTTCAATGTCTCGTCATTATTTTCAATTTTCTGCAAAAACATTCTAGACTCATCGAATGTCACATCTCGACTATTAACAATCTTACTTGTTTCTGGACAACATAACCTATGGCCCTTCACGCCTTTGCTAAACCCCATAAACTTGGCTTTTTTTGCTCTAGGATCCAGCTTTGAGTCTGTCACATGATAATAAGCAAGACACCCAAACACATGCAATTTATTAACGGGAGTACCTGACCATACCTCAAGTGGGGTTTTTCCACCATTTCCAGAGACAGGTAAACGATTCACCAAGTGAACTGCATAACTGAGAGCCTTAGCCCAAAAAGCCTTAATCCTACTTGAGACAATATGCATCGAACCTTCTCTATTAATGTCTGGTTCATTCTCTCACTTCATCTCGGCATACTTTAAGGAATGGATCATAAGTATATTCTCCACCGTTGTCTGATCTCAACTTTTTGATTTTCCTGTTCGTCTGATTCTCTACCATTTTTTTCCACTCTAGGAAGATTTGGAGAACCTCGTTTTTGTGCCTCATGGGATACATCCAAACTCTCCTCGAGTAGTCATCAATAAAGGTGACAAACCACCTCTTTCCTCCCAATGAGACATTCTTCGTGGGACCGTATACATCAGTGTGAACATAATCCAAAATGCCCTTTGTTTGGTGTATAGTTGTACCAAACTTCACATTTTTTTGTTTACCAAATATACAATGTTCACAGAAATCAATTTGACCTGTTGTGGCACCCTTAAAGACTCTTGGTTCACCAATGTCTGAAGCGCCTTCTCACCAGCATGCCCGAGTCGCATGTGCCATAATTTCGATATGTCTTGTTCTTTCGAACTTGCCACAGCAGCATGCCCAATTATTGTGGTTCCGTTGAGGTAGTATATGTTACCTCTTCTAGTTCCTTTAATTGCCACCAAAGCTCCACGAGCTACTTTCAAATTCCCTCTTTCCAAAATGATGCGATAACCACTCGCATCCAAAACGTCAAGGGAAATCAAATTTTTCTTCAAGTCAGGGACATACCTCACATCATTGAGTTTTCGTAGAACCCCGTCAAACATCTTGATTTGTACTGAGCCAATTCCTGTCATCTTGCAAGTACTATCATTGCCCATATAGACAACTCCACCATTGAACTCTTTGAAGTCCAAAAACATCTCTGTTGTAACACATATGATAGAAGCACCCAGAATCAAGAATCCATTCACGGGTTTGGCTGACTGATGAGGAGATCGTCAAGACATTATCTGTATCAGTGTCGTCATCTCTAACCACATTGGACTTCGACCCCTTCTCTTTTAAGATCGGGCAATCTTTCTTCCAATGGCCTTTCTGTCGACAATATGCACACTCATTTTTATCAAGTTTTCTCCAATTACCATATTTACCTCTTGACTTTGATCGGGACTCTCCACATTCGCTCCTTTTCCAAGTAGAAGTCCCTCCACGAGCAGTGAGAACATTTGAGCTGGAATCTTGATATGCCTTCTTTTCCTTCTTTCGCACCTCATTATTCATTAGAGCATTTGAAACATCTTCAAAATCAATATCAGAGGCTCCATGCAGTAGAGTGGTTACTAAAAACTCGTAGGACTCCGGCAAAGAATTTAATAACAATAACGCCTTGTCTTCGTCATCAATCTTAACATCAAGATTGAGCAAATCGGTGATGATTTTGTTAAAATCATCTAAATGTTCAGCCATTGAGATACCTTCCTTGTAGTCAAAACAGAAGAGTTTCTTCTTCAAATAGAGTTTATTTTCCACACTCTTCTGCATATATTTCGCCTCAAGCTTGTCCCATAGTTCTTTTGCCATGGTCACCTTCATAAATGGATATTTTTTATCTTTGCCAAGACAGGATCGAATAATTCCGCAAGCTTGCCGATTTAATTTCATCCATTCTGCTTCTGTCATGTCTTCAGGCATCATCTCTTGGAGAGCAGCATCAAGATCTTGCATGACCAACAAATTGCTAACTTCACCTCGCCAAACACCAAAATTATTGGTTCCATCAAATTTCTCTACTTCGAGATTAGAACTCCACAATCTCGATCCACGCCGTGATTTAATTCCAGATGGCTCTGCCATAAGCACGATTGTGAGCACGTCTAGTGGTCGATGAGGCCAAAACTTTCACAATACCCCAAATACCACAATCAGGCTCTGATACCAATTTGTTGTGCGCCGGAAGCGGAACGAATGTACTCACAACACCAATGAACAGAATCACACAAAGAGACACCAAGATATTTACGTGGTTCGGAGAGAAGAAGTTCCCCTACATCCATGGGTAGCAATCAATCACTAAAACCCAAGTATTACAAGTGAATACCTCACTATCACACTACAAAGTCTCTCACCAATGTGTCACCCAACAAATACAATGTGACACCCAAAACTCTCCCAACTCCCGAATCCCACTTGTTGTCAATCGATATCCAGAATGGAGGGAGAACTCTCTCATTATATAGCGTCACAAATCAAATCTCTGACTGAGACTAAATCTAATTCAAATAACCAATCAAATCTAATTCAAATCCAAAAACCAAATCAAATCTTATGACAATTTACAACACAAGTATTCAAGAAACATATATGAAAGATGCAACTTATAGGCTTATTATCACACTTGTGCTGCAACGCGAAGCATTGTTATGCTTTAGAACTCAAGGTCGCAGCCCATAGAACACATCCTGTTCAAGTAGTACTACAAGGTCTTTTCTTATATAAGCTGAGTATAAAACCTTGTGTGGGTCTTGCACCATTATCTGTCAGAGGTGTGAACACCTGTGCTTCTTTTTTGGCATTGTAATACGAAGCCTTGTGGTTGCATGCTCTTTTTTTAATCAAGTATATTTCTTCTTGGCTTATCCATTTGTAAAATTCTTTCTGAACTTAGTTACTCTTCAAGTTTTTGCTTGAACAGGTAAAAGAGGGCATCACAAATATTGGGGAACAAGGATGTTTAAATACGGCGATCAAGATGTGTTGCATTTTCTTCTCTCAAATCTTAACTGGTACAGTGTTGCATTTTCTTCTCTCAAATCTTAACTGGTAATGTACAAAACAAACCTCAAGGCTATTTTCCTGGTATGATTGAAAGTGATATTCATTCGTTTCCCCCTTTTTAGAATTGTTCATCGCCTTTCCTTCTTTGTTTACAATATTTTAAGGGGAGTTTTGATCCTCTGTTGCAAGAACATGTTCTTAGATAGAATCCTATAGGGAACGATGGGAAATCTTCTCCCATAGTGAGTGAATAATTACAGTCTAGATGGAAAGGACCAAAATTTTGTTAATCCCTTTTTCTTCATACATCCTTGTACATATCATGTTGATCGAAATGGGTTACATCTGTCTTGGAGTACGATTGTTTCTCCATGATTGAGGAGATTCTCTTTTCTTTTTCTTTTTGTGAGAAAGGCTGTGTGCTATGGCAGACGAGGTTTTTTTTTGCTATTGTTTGGGGTATTTGACTAGAGAGGAATAACAAGACTTTTAGGGAGGTTGAGAGATCCAATGAAGAGGTCTAGGAGGGGGCTCGGTTTAATGCCTCATTGGGGGTTAGTCTCTTTTGTAATTGTGATCTTGGTTTGATTTAGTTGGATTGGAGCCCCTTTGTAGCTAGTTTGGGGCTCCCTTCGTTTTCTTGTTAACTATTTTTTTTTTGTATTCTCCTATTCTTTTATTATTTCTCAATGAAAGGTAGGTTTCTTATATATATATATTGAAATGAAATTTATGTTCCAACTTCTGTTTTGTATGCAATAGTTGTTTGACATTATGTTTTCTTTCTATTGTATATGAAGCGCGTGCTTTTGTTTATTGGTATGGTCATGTTAACTAGTTAGCATGGACCTTCTACATATGTAATTATTAATCCGCATGTAGGTTTATCTTTTAACAGGTGGGTTGTGGAGTATCGTATTGATGGTTTCCGTTTTCATTCACTCTCTTCAATGATCTATACTCACAATGGGTTTGCTTCTTTTACCGGAGACATGGAAGAGTAAGGCATCTACTGGAACTAGTAATCTTACATACACGCAACTGCTCTGTTATCCTTCTGCATTTCTTTTTAATGCAATTTGAAACTAAAACTTTAATTCGGAAAGGAAAGCAATAATACTGATAACTTGGAAAATAGCTCTAGATGGATCTTTATTTGTTTGATTCAAACCATGTTTATTGTCTCATTATAACATATGAACCAGAGATCTTCTCTCAATTATCCTTGTTTAACTGCTTTTAATAAAAGTTATTCCAACTGCACGGAAAGTTTGTGTGTATCTTCAGCTTAACGTTAGTATTTTGGTTTCTGATTTATGCTATCCTTGTGGTAATTGAAACCCAACCTGTTTGTTCACAGGTTCTGCAATCAATATGTAGACAAGGATGCGTTGCTATACCTCATGCTGGCAAATGAGATACTTCATGGTCTTCATCCTAATATAATAACGATTGCTGAAGATGTAAACAATTGCTAATGTATTTGCTTTTGATATAATTTTATCATACCTATACTTGAAAACCCTTTTATTCTACACTTTGTAGTTAAAGTTAGAGGTTGGATTAATACCTTGTTTTGGCTTGGTAAATGGTTAATCGATGGTCTCTAGCTGATTAATTCTTTAGACTCTTCATGCTGATTAATTCTTTAGACTCTTGGCTAGGATATTTACTTTTAGGCATTATATACATTCTAGCTTTCTAAAAGTTCTTATTTGTCAGGCAACGTATTATCCTGGACTATGTGAGCCGACCTCTCAAGGTGGACTGGGTTTTGATTATTATGTGAATCTTTCCGCATCCGAGATGTGGTCGTCCTTCCTTCAGAATGTTCCAGACCAGGAATGGAGCATGAATAAGGTTGTTTCTATACTTTGTGAACCTGTTTGAATCACTTGAGGGTTTTCCATTTATTAAGGGGAGAGAATTGCATCATGCAGATTGTTAGCTCATTAATTGACAACAGACACTACGTGAACAAGATGCTCCTGTATGCTGAAAACCACAGCCAAGTAATTTTCTTCTCAACCTTTTTAAATTAACGATGATCCGTACCTTATGAATTTTAAGCATGGGTTTTTCATGCTTGGTTGCGTATCTTCCTGATCTTTTTCGTTGTTTCTTTATTCTTTTAGTCCATCTCTGGTGGACGTTCGTATGCTGAGATTCTATTTGGTGATACCAAGGAGCATGGTTCTGGATCAAAGGAAACACTGCTCAGGGGGTGTTCCTTACACAAAGTATGATTCATCTATTGAAGTAAAATTATTCATTTAACATCTTAGACAATAAATGTTCTAGTTTGCATAAAATTCTTGTAGCTTCAGAAACAGTTGCAATTTCACGATTTTACCGGTTTCAAATGTTTAGTTAGCCAATAATTGGAAATTAGAACGTAGATCAAATGATATGTTGACCGAACAACATTTCAACTAATCACTTTCGGTTTTTACATTAACTACATTATAATCTTTTATTACAAAAACTGTTGAAATATCATCACTAGCGTCAAACCCAATTATGGTAAGAGCCCATATATTTATTTTAGTAGATATTTTAGGAGAAAGAGATCTAGATATTTTTAGGAGAAAGATTCTTTGTTAGGTCTCCAATTCAAATCTGTTAGGTCTAGATTTAGATTGGTTAAATATAGATTGTTTACTCTTAGTATAAATACCCACCCTACCTAGGTTATTCATCCAAGCAGAAGTATTCTACAGAGTGTCTTGTATTCTCTTCTCGATTGGTCTTAATAAAGAGTGCTAGTGTTTCCCATTCAACAAAAACTCAGCCTCTTAAAAAAGAACGGTCTATCCAAGGTCCATATCATGTGTCTATGCCCATTTTCAATGTATATTAGCTTACATTCAAATGCATAGGGTGAACTAGATACTGCAGAAAGAGTTCTACGTTCTATTCCTTTCTGTCCTTACATTCAAATGCATAGCATAAGTTCTATCTCTGTAATTTTTCTTTTGATGGCCTTGAATCTGAATCAGATGATTAGATTTATCACATTTACTATGGGCGGACGAGCATATCTGAACTTTATGGGAAACGAGTTTGGGCACCCGAAGGTCAGGTTCTATCTCTTCAATATGCATGAAGATGTATAATATTTGTAATATTTGTTTCCTGTCAAAATATATGTGTAATATTTGTCTATGTATCAGAGAGTTGAGTTCCCGATGCCAAGCAACAACCTCTCGTTTTCACTTGCCAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCGTGATTTGTTCGTCTTTGACAAGGTGTTCAGCTATTTTTCAATATCATAAATATTGAAAACTTTGTTATGCAACTTGAAGTGAAGTAAATGTTCTTGGCATTAAGTCTCACAAATATTTTATCATCCAATCTTAGTTTAATAATTTTCTTTGGTAAAAAATCGTGCAATCAACAAAGAAATTAGACTATAACCAACTCCGGAAAGTGACTCTAATTCAAAATAATTAAACTAAAATCATAATTGTAAAAAGGATTCTTGACCGATGCTTAAAAGGAGACATCCTCACCACCTTCCAAACCTCATCACATGATGTTTAACTTAATTTAATAAAAAATGAGTTTCTATTGATCTAATTCAATTGTGCTAATTTACTTTCTTCTTTCTAGGACCTGATGGGATTGGATGAAAATGAAAAACTACGAACAAGAAGTTTACCAAATGTTCACCATGTCAATGAAGCTACCAAAGTAAGATTTATCCTTTTCCTTTCTTTTTTAGTTCATTTTATCTACGGACGCTTACACGTTTGGTTCTTGCTTATCTAACGCACCCTTAAGAATGCAGATAAATGTAATTCTTGGATGGAACATCTTGATATAGTGTTCAGTGTGAATCAATACATTATTTTTAACCTTTTCAGGTAATATCTTATATTCGAGGTCCCTTCCTCTTCATATGCAACTTCCATCCCACAGATTCTTACGAAAGATACAGTGTTGGTGTGGAAGAAGCTGGAGAATATCGAGTAAGTACTACTTGGATCTTGTTCGATAACGATTATCTTTCGTCTTAAAATTGTTTTTTCACTTTTATAAAACATTTTTAGAATGTACACAATTTAAACTAATAACTCAAACCATTTTAAAAGCAAGTTATAATTTTAAAATAGATTAGAAAATAAAGAACCTAAGAATATCTCAATTGTTAAATCATATACCCTCGATCTGATAAGAGGTTCTGTGAGATCTCACATCAGTTGGGGAGGAGAATGATGCATCCTTTATGAGGGTGTGGAAACCTCTCCCTAGTAGATGCGTTTTAAAAGCCTTGTGGGTTTGGGCTGTTACAGTTGGTATTAGAGCCTGACACCGGGTGGTGTGCTAGCGAGAACATAGAGGGTGGACATCGGGTGGTGTGCCAGCGAGGACACTGGCCCCAAAGGGGGGTGGATTGTGAGATCCCACGTTGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGATTTCGAAACCTCTCTCTCGTAGACATTTAAAAACCTTAAGGGAAAACCCCAAAGAGGACAATATATGCTCGCAGTGGCCTTGGGCTGTTACAGGTTCGAATCTCTCCCTCCCCCTACATGTTGTGTGGCTGGACTTTAATAAACAATGAACTTGAATTTGAAGTTATTATCAAAGGGGGTGTGACCTTTTGCTTATTATCTAGCACCCATGTATACACATTGTCACTCCATGAACTTTATACAAATAGGCAAATTTTAGCCTTTAAACAAAAGTCTTCCAGTCCTCATATTAACCATAATTTGGTTTTTGTGCTCATTTTCAAACACCCAAAAAATGGACTAAAGTCTGATATTTTAATGTGCACAGATTATATTAAATACCGATGAGACTAAATATGGAGGTCAAGGGATTATAAAGCATGATCAATATCTTCAACGAACAATAAGCCGAAGGTAATGGTTTTCGACTTTCTTGAAGACTTCCTTCGTTCTCCTGTAATTCATTTTGAGATTACTTTTCACCATATATCATCATATGATCTTCATGTTTCCAGAATTGATGGCCTCCGAAACTGCCTAGAAGTGCCGTTGCCTTGCAGAACTGCACAGGTTCGTTAAATTTGTGGATGTTGTGTTGTTTTTCGGATATGGTTTTTTGTTTCTCGTTTCCTCTTTGTCAAAAAATAGAAACATGTTTGGAAACCATTTCTTGTTTCTTGTTTTTTGGAATTCTGAATAAAAAAGAATGATTGATTCATCACGTTATGATAGGCAAACGAAAAAAAATGAAGTAGATAACTTAAAATATTAGAATAACGTGATCTATCAACGAGCGTATGTTACATATGGTACTCTACTTGTTCTCTGTTTTTTTCTGTTATTTATCCGTTAACATAACATATAATCACGGTTGAAAGTTCAAGAACATTATTGAAACTTGTTAAAGTTCGGTGACTAATATACACGACCGCTAGACTTCATTTATGCTCGAAAATGATGCAAAGGAACTGATATTACACTTGCTTAAGATACTCTGCACACGCCTCGTTAATTTTCGAACTAAATTTATTTTCCATGTATACTCACCCGATCTCTCTCGCTCGCTCGTGCAGGTCTACAAACTAAGTCGAATATTGAGAATATAAGCCGTTTTGAACCCATTGGAAGAAATTAGACAGAAGTTTGCCAAAGTTGCTAATATGTGTCTGCTAGAAAGGCATGCCCAAAAGAGCCTGATTTTCCAGTGGTGAGGTAATTTGTTGAATCAAAGCCTGTTAGAACAGCTGATGTTCAATGTATTTATGAGATGAAATCACTTGATCTTTGCAGGTTTCATTTCCTATTTTCACCACTCATGGAATTTTGACTGTGGCTCTTTTCTAAACAGCCATGGTAGATTTGTATATTAGGTTGATGATGAGTAAGTGTTCACTTTATACTCTATACTTTATTACCCGCCCATTTATTTCTTACCTAAATTTTTATTTTGTAAGAGAAGCGAAACATTTCTTATAATGTTGTAGAAACTTCTCCCTAGTAGACATGTATTAAAACTAGATGCTGATGATGATACATAACGGGCCAAAACAGACAATATCTAATGATAGCGGTGAATTTGGATTGTTATAAATGGTATTAGTGCTAGACATCGACGGTGTGTCAGCAAGGACGCTGGGTGTCA

mRNA sequence

ATGAACCAGCACAGTTTCCCCGCCATGAACGCCCTTTCAAGTTCACCTTCCCTCAAATTCCCTCCTTACAAGACCAATCATCTCCTTCTACTACCTCACTCCCGCTCCACGCTTCAATCCATCGCTCTGTCTCGGAAACTAAAATTGAAGATAATTTGTTCAGCATCCGAACATTCACAACATGCTGAACCAGCGAAGTACCAGCACTCTAAGAAGACGAAAAGCGTCTCGGATGGCGAGAAAGGGGTTGATCCTGTTGGATTTCTCACTAAACTCGGCATTTCTCACAAGCAATTTGCTCATTTCCTCCGCGAAAGGACCTCAAGGATGAAATTTACGATCGTCATGCGAATCTCCAGGACCTGTCTTCTGGGTATGCATCGCCATGTGGAACATCGTGTTGATTTTATGGAATGGGCTCCAGGTGCTCGCTATTGTGCAGTAATTGGTGACTTCAATGATTGGTCACCCAGAGAAAACGTTGCCAGAGAGGGGCATCTTGGCCATGATGATTATGGTTACTGGTTTATCATCCTCGAAGATAGGTTGAAGGAAGGAGAGAAACCAGAGGAACTCTATTTTCAACAGTATAATTACGTGGATGATTATGATAAAGGTGACAGTGGAATCAGCATTGACGAGATCTTCAAAAGAGCGAATGATGAATATTGGGAACCTGGAGAGGACAGGTTCATCAAAAACCGTTTTGAAGTTCCAGCTAAGCTCTATGAAGAATTATTTGGCCCCAATGGTCCTCAAACATTAGAAGAGCTTGAAGAAATACCAGATGCAGAAACAAGATACAAGGCATGGAAAGAACAACATAAAGATGATCCATCTAGTGTTTCATATGACGTGATTGATAATGGGAAGGAGTATGATATTTTTAACGTGGTGATTGATCCCGTAACAAGGGAGAAATTTAAGGTTAAGAAACCTCCTATACCTTACTGGTTAGAAACACGTAAGGGAAGGAAAGCTTGGTTAAAAAAGTATAATCCCACTATTCCACATGGAAGCAAATACAGGGTATATTTCAACACTCCTGATGGGCCATTGGAAAGAATTCCTGCTTGGGCTACTTATGTGCAGCCAGATGCTGAAGGGAAGCAAGGTTTTGCAATCCATTGGGAACCACCACCAGAATATGCATATAACTGGAAAAACACCAGGCCTAATGTACCAAAGGCCTTGAAAATATATGAATGTCATGTGGGAATTAGTGGACCAGAGCCGAAAGTTTCTTCTTTCGACTACTTTAGAGAGAAGGTTCTTCCACATGTGAAGGAAGCTGGATATAACGCAATCCAACTGTTTGGAGTGGTTGAACATAAAGATTACTTTACACTTGGCTATAGGGTTACTAACTTCTTTGCTGTCAGCAGTAGATATGGGACCCCTGAGGACTTCAAGCGCTTGGTTGATGAAGCTCATGGCCTTGGACTTTTTGTCTTCTTGGATATTGTTCATTCATATGCTGCTGCTGATGAGATGGTCGGACTGTCATCATTTGATGGATCAAATGACTGCTACTTTCATACTGGTAAAAGAGGGCATCACAAATATTGGGGAACAAGGATGTTTAAATACGGCGATCAAGATGTGTGGGTTGTGGAGTATCGTATTGATGGTTTCCGTTTTCATTCACTCTCTTCAATGATCTATACTCACAATGGGTTTGCTTCTTTTACCGGAGACATGGAAGAGTTCTGCAATCAATATGTAGACAAGGATGCGTTGCTATACCTCATGCTGGCAAATGAGATACTTCATGGTCTTCATCCTAATATAATAACGATTGCTGAAGATGCAACGTATTATCCTGGACTATGTGAGCCGACCTCTCAAGGTGGACTGGGTTTTGATTATTATGTGAATCTTTCCGCATCCGAGATGTGGTCGTCCTTCCTTCAGAATGTTCCAGACCAGGAATGGAGCATGAATAAGATTGTTAGCTCATTAATTGACAACAGACACTACGTGAACAAGATGCTCCTGTATGCTGAAAACCACAGCCAATCCATCTCTGGTGGACGTTCGTATGCTGAGATTCTATTTGGTGATACCAAGGAGCATGGTTCTGGATCAAAGGAAACACTGCTCAGGGGGTGTTCCTTACACAAAAGAGTTGAGTTCCCGATGCCAAGCAACAACCTCTCGTTTTCACTTGCCAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCGTGATTTGTTCGTCTTTGACAAGGACCTGATGGGATTGGATGAAAATGAAAAACTACGAACAAGAAGTTTACCAAATGTTCACCATGTCAATGAAGCTACCAAAGTAATATCTTATATTCGAGGTCCCTTCCTCTTCATATGCAACTTCCATCCCACAGATTCTTACGAAAGATACAGTGTTGGTGTGGAAGAAGCTGGAGAATATCGAATTATATTAAATACCGATGAGACTAAATATGGAGGTCAAGGGATTATAAAGCATGATCAATATCTTCAACGAACAATAAGCCGAAGAATTGATGGCCTCCGAAACTGCCTAGAAGTGCCGTTGCCTTGCAGAACTGCACAGGTCTACAAACTAAGTCGAATATTGAGAATATAAGCCGTTTTGAACCCATTGGAAGAAATTAGACAGAAGTTTGCCAAAGTTGCTAATATGTGTCTGCTAGAAAGGCATGCCCAAAAGAGCCTGATTTTCCAGTGGTGAGGTAATTTGTTGAATCAAAGCCTGTTAGAACAGCTGATGTTCAATGTATTTATGAGATGAAATCACTTGATCTTTGCAGGTTTCATTTCCTATTTTCACCACTCATGGAATTTTGACTGTGGCTCTTTTCTAAACAGCCATGGTAGATTTGTATATTAGGTTGATGATGAGTAAGTGTTCACTTTATACTCTATACTTTATTACCCGCCCATTTATTTCTTACCTAAATTTTTATTTTGTAAGAGAAGCGAAACATTTCTTATAATGTTGTAGAAACTTCTCCCTAGTAGACATGTATTAAAACTAGATGCTGATGATGATACATAACGGGCCAAAACAGACAATATCTAATGATAGCGGTGAATTTGGATTGTTATAAATGGTATTAGTGCTAGACATCGACGGTGTGTCAGCAAGGACGCTGGGTGTCA

Coding sequence (CDS)

ATGAACCAGCACAGTTTCCCCGCCATGAACGCCCTTTCAAGTTCACCTTCCCTCAAATTCCCTCCTTACAAGACCAATCATCTCCTTCTACTACCTCACTCCCGCTCCACGCTTCAATCCATCGCTCTGTCTCGGAAACTAAAATTGAAGATAATTTGTTCAGCATCCGAACATTCACAACATGCTGAACCAGCGAAGTACCAGCACTCTAAGAAGACGAAAAGCGTCTCGGATGGCGAGAAAGGGGTTGATCCTGTTGGATTTCTCACTAAACTCGGCATTTCTCACAAGCAATTTGCTCATTTCCTCCGCGAAAGGACCTCAAGGATGAAATTTACGATCGTCATGCGAATCTCCAGGACCTGTCTTCTGGGTATGCATCGCCATGTGGAACATCGTGTTGATTTTATGGAATGGGCTCCAGGTGCTCGCTATTGTGCAGTAATTGGTGACTTCAATGATTGGTCACCCAGAGAAAACGTTGCCAGAGAGGGGCATCTTGGCCATGATGATTATGGTTACTGGTTTATCATCCTCGAAGATAGGTTGAAGGAAGGAGAGAAACCAGAGGAACTCTATTTTCAACAGTATAATTACGTGGATGATTATGATAAAGGTGACAGTGGAATCAGCATTGACGAGATCTTCAAAAGAGCGAATGATGAATATTGGGAACCTGGAGAGGACAGGTTCATCAAAAACCGTTTTGAAGTTCCAGCTAAGCTCTATGAAGAATTATTTGGCCCCAATGGTCCTCAAACATTAGAAGAGCTTGAAGAAATACCAGATGCAGAAACAAGATACAAGGCATGGAAAGAACAACATAAAGATGATCCATCTAGTGTTTCATATGACGTGATTGATAATGGGAAGGAGTATGATATTTTTAACGTGGTGATTGATCCCGTAACAAGGGAGAAATTTAAGGTTAAGAAACCTCCTATACCTTACTGGTTAGAAACACGTAAGGGAAGGAAAGCTTGGTTAAAAAAGTATAATCCCACTATTCCACATGGAAGCAAATACAGGGTATATTTCAACACTCCTGATGGGCCATTGGAAAGAATTCCTGCTTGGGCTACTTATGTGCAGCCAGATGCTGAAGGGAAGCAAGGTTTTGCAATCCATTGGGAACCACCACCAGAATATGCATATAACTGGAAAAACACCAGGCCTAATGTACCAAAGGCCTTGAAAATATATGAATGTCATGTGGGAATTAGTGGACCAGAGCCGAAAGTTTCTTCTTTCGACTACTTTAGAGAGAAGGTTCTTCCACATGTGAAGGAAGCTGGATATAACGCAATCCAACTGTTTGGAGTGGTTGAACATAAAGATTACTTTACACTTGGCTATAGGGTTACTAACTTCTTTGCTGTCAGCAGTAGATATGGGACCCCTGAGGACTTCAAGCGCTTGGTTGATGAAGCTCATGGCCTTGGACTTTTTGTCTTCTTGGATATTGTTCATTCATATGCTGCTGCTGATGAGATGGTCGGACTGTCATCATTTGATGGATCAAATGACTGCTACTTTCATACTGGTAAAAGAGGGCATCACAAATATTGGGGAACAAGGATGTTTAAATACGGCGATCAAGATGTGTGGGTTGTGGAGTATCGTATTGATGGTTTCCGTTTTCATTCACTCTCTTCAATGATCTATACTCACAATGGGTTTGCTTCTTTTACCGGAGACATGGAAGAGTTCTGCAATCAATATGTAGACAAGGATGCGTTGCTATACCTCATGCTGGCAAATGAGATACTTCATGGTCTTCATCCTAATATAATAACGATTGCTGAAGATGCAACGTATTATCCTGGACTATGTGAGCCGACCTCTCAAGGTGGACTGGGTTTTGATTATTATGTGAATCTTTCCGCATCCGAGATGTGGTCGTCCTTCCTTCAGAATGTTCCAGACCAGGAATGGAGCATGAATAAGATTGTTAGCTCATTAATTGACAACAGACACTACGTGAACAAGATGCTCCTGTATGCTGAAAACCACAGCCAATCCATCTCTGGTGGACGTTCGTATGCTGAGATTCTATTTGGTGATACCAAGGAGCATGGTTCTGGATCAAAGGAAACACTGCTCAGGGGGTGTTCCTTACACAAAAGAGTTGAGTTCCCGATGCCAAGCAACAACCTCTCGTTTTCACTTGCCAACCGACAGTGGGATCTTTTAGAAAAGGAAATGCATCGTGATTTGTTCGTCTTTGACAAGGACCTGATGGGATTGGATGAAAATGAAAAACTACGAACAAGAAGTTTACCAAATGTTCACCATGTCAATGAAGCTACCAAAGTAATATCTTATATTCGAGGTCCCTTCCTCTTCATATGCAACTTCCATCCCACAGATTCTTACGAAAGATACAGTGTTGGTGTGGAAGAAGCTGGAGAATATCGAATTATATTAAATACCGATGAGACTAAATATGGAGGTCAAGGGATTATAAAGCATGATCAATATCTTCAACGAACAATAAGCCGAAGAATTGATGGCCTCCGAAACTGCCTAGAAGTGCCGTTGCCTTGCAGAACTGCACAGGTCTACAAACTAAGTCGAATATTGAGAATATAA

Protein sequence

MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMKFTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGCSLHKRVEFPMPSNNLSFSLANRQWDLLEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLSRILRI
Homology
BLAST of Carg12832 vs. NCBI nr
Match: KAG7024931.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1805.8 bits (4676), Expect = 0.0e+00
Identity = 863/863 (100.00%), Postives = 863/863 (100.00%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMKFTIVMRISR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMKFTIVMRISR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMKFTIVMRISR 120

Query: 121 TCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIILE 180
           TCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIILE
Sbjct: 121 TCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIILE 180

Query: 181 DRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEVPA 240
           DRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEVPA
Sbjct: 181 DRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEVPA 240

Query: 241 KLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVI 300
           KLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVI
Sbjct: 241 KLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVI 300

Query: 301 DPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERIPAWA 360
           DPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERIPAWA
Sbjct: 301 DPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERIPAWA 360

Query: 361 TYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSSFDYF 420
           TYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSSFDYF
Sbjct: 361 TYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSSFDYF 420

Query: 421 REKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGL 480
           REKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGL
Sbjct: 421 REKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGL 480

Query: 481 GLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVWVVEY 540
           GLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVWVVEY
Sbjct: 481 GLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVWVVEY 540

Query: 541 RIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITI 600
           RIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITI
Sbjct: 541 RIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITI 600

Query: 601 AEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHY 660
           AEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHY
Sbjct: 601 AEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHY 660

Query: 661 VNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGCSLHKRVEFPMPSNNLS 720
           VNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGCSLHKRVEFPMPSNNLS
Sbjct: 661 VNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGCSLHKRVEFPMPSNNLS 720

Query: 721 FSLANRQWDLLEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPF 780
           FSLANRQWDLLEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPF
Sbjct: 721 FSLANRQWDLLEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPF 780

Query: 781 LFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLR 840
           LFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLR
Sbjct: 781 LFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLR 840

Query: 841 NCLEVPLPCRTAQVYKLSRILRI 864
           NCLEVPLPCRTAQVYKLSRILRI
Sbjct: 841 NCLEVPLPCRTAQVYKLSRILRI 863

BLAST of Carg12832 vs. NCBI nr
Match: KAG6592053.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1739.5 bits (4504), Expect = 0.0e+00
Identity = 847/909 (93.18%), Postives = 850/909 (93.51%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+IICSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWDLLEKE
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. NCBI nr
Match: XP_022937279.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita moschata])

HSP 1 Score: 1723.4 bits (4462), Expect = 0.0e+00
Identity = 839/909 (92.30%), Postives = 845/909 (92.96%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+I+CSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EP+VSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPEVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL VFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWDLLEKE
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. NCBI nr
Match: XP_023535701.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo] >XP_023535702.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo] >XP_023535703.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1713.4 bits (4436), Expect = 0.0e+00
Identity = 832/909 (91.53%), Postives = 842/909 (92.63%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFPAMNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+I+CSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCA+IGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL VFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWD LE+E
Sbjct: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           +HRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 IHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. NCBI nr
Match: XP_022976653.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita maxima] >XP_022976654.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita maxima])

HSP 1 Score: 1699.9 bits (4401), Expect = 0.0e+00
Identity = 827/909 (90.98%), Postives = 837/909 (92.08%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFP MNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+IICSASEHSQ
Sbjct: 1   MNQHSFPPMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQ AHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQLAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCAVIGDFN WSPRE+VAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNGWSPREHVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFK KKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKAKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL V LDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVLLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWDLLE++
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEEK 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           MHRDLFVFDKDLMGL ENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 MHRDLFVFDKDLMGLGENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHD YLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDHYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. ExPASy Swiss-Prot
Match: D2WL32 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE3 PE=1 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 614/905 (67.85%), Postives = 708/905 (78.23%), Query Frame = 0

Query: 9   MNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KLKI C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK----------FTIVMRI 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER   +K          F      
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           S   LLGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LED+L+EGE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AY WK ++P VP++L+IYECHVGISG EPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGL VFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 V----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           V          W+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHK--------------------------RVEFPMPSNNLSFSLANRQWDLLEKEMHRD 788
           SLHK                          RVEFP  SNN SFSLANR+WDLLE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVG 848
           LF FDK+LM LD+++ + +R LP++HHVN+A  VIS+ RGPFLFI NFHP++SYE+Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 864
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 899

BLAST of Carg12832 vs. ExPASy Swiss-Prot
Match: Q8NKE1 (1,4-alpha-glucan-branching enzyme OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=GLC3 PE=2 SV=2)

HSP 1 Score: 422.9 bits (1086), Expect = 8.5e-117
Identity = 236/643 (36.70%), Postives = 355/643 (55.21%), Query Frame = 0

Query: 258 LEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVIDPVTREKFKVKKPPIPY 317
           L  +P+ +  Y+ W       P++V+  +I    ++D       P+ ++ F V +  IP 
Sbjct: 63  LNVLPNGDIIYREWA------PNAVAASLIGEFNDWDRSK---HPMKKDSFGVWEVHIP- 122

Query: 318 WLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGF-AI 377
                       K   PTIPH +K ++   TP+G  ++R+PAW   V  D      + AI
Sbjct: 123 -----------AKNGIPTIPHNTKIKISMTTPEGECIDRLPAWIKRVTQDLNVSLAYDAI 182

Query: 378 HWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNA 437
            W PP +  Y WKN  P  P +L+IYE HVGIS  E +V +++ F + VL  +K+ GYNA
Sbjct: 183 FWNPPQK--YQWKNNSPKKPTSLRIYEAHVGISTNEGRVGTYNEFTDNVLKRIKDLGYNA 242

Query: 438 IQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAA 497
           IQL  ++EH  Y + GY+VT+FF VSSRYGTPE+  RL+D AHG+GL+V LD+VHS+A  
Sbjct: 243 IQLMAIMEHAYYASFGYQVTSFFGVSSRYGTPEELMRLIDTAHGMGLYVLLDVVHSHACK 302

Query: 498 DEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGF 557
           + + GL+ FDGS+ CYFH G +G H  W +R+F YG  +V          ++ EYR DGF
Sbjct: 303 NVLDGLNMFDGSDHCYFHEGGKGRHDLWDSRLFNYGHWEVLRFLLSNLRFFMEEYRFDGF 362

Query: 558 RFHSLSSMIYTHNGFAS-FTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDA 617
           RF  ++SM+Y H+G  + F+G   E+    VD+  ++YLMLAN++LH L+P IIT++ED 
Sbjct: 363 RFDGVTSMMYHHHGIGTGFSGGYHEYFGDTVDEGGVVYLMLANDMLHKLYPRIITVSEDV 422

Query: 618 TYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKM 677
           +  PGLC P  +GG+GFDY + ++  +MW   L+   D +W M  I  +L + RH + K 
Sbjct: 423 SGMPGLCLPVEEGGIGFDYRLAMAIPDMWIKLLKEQRDDDWDMGNICWTLTNRRH-MEKT 482

Query: 678 LLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLL-----RGCSLHKRV--------- 737
           + YAE+H Q++ G ++ A  L  D + +   S  T L     RG +LHK +         
Sbjct: 483 IAYAESHDQALVGDKTLAFWLM-DKEMYTHMSDMTPLTPIIDRGLALHKMIRLLTHGLGG 542

Query: 738 -----------------EFPMPSNNLSFSLANRQWDLLEKEMHRDLFV--FDKDLMGLDE 797
                            +FP   NN SF  A RQW++++ ++ R  ++  FDK +  L+E
Sbjct: 543 EGYLNFEGNEFGHPEWLDFPRAGNNNSFHYARRQWNVVDDDLLRYKYLNEFDKAMQHLEE 602

Query: 798 NEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNT 855
                +     +   +   K++++ RG  L+I NFHPT S+  Y +G E AG+Y I LNT
Sbjct: 603 QYGWLSSPQAYISLKHNENKLVAFERGNLLWIFNFHPTQSFADYKIGTEWAGKYSIALNT 662

BLAST of Carg12832 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 413.3 bits (1061), Expect = 6.7e-114
Identity = 213/541 (39.37%), Postives = 311/541 (57.49%), Query Frame = 0

Query: 334 PTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAE--GKQGFAIHWEPPPEYAYNWKNT 393
           P IPH S+ +  F   +G  ++RIPAW  Y   DA         ++W+PPP   Y++K  
Sbjct: 199 PVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPPPSERYHFKYP 258

Query: 394 RPNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTL 453
           RP  P+A +IYE HVG+S  EP+V+S+  F + VLP +K   YN +QL  ++EH  Y + 
Sbjct: 259 RPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMAIMEHSYYGSF 318

Query: 454 GYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFD---GS 513
           GY VTNFFAVSSRYG PED K L+D+AH LGL V +D+VHS+A+ +   GL+ FD   GS
Sbjct: 319 GYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDGLNGFDIGQGS 378

Query: 514 NDCYFHTGKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTH 573
            + YFH G+RG+HK W +R+F Y + +V          W+ EY  DGFRF  ++SM+Y H
Sbjct: 379 QESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRFDGITSMLYVH 438

Query: 574 NGF-ASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQ 633
           +G    FTG+  E+ ++  D DA++YLMLAN ++H + P+   IAED +  PGL  P S+
Sbjct: 439 HGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSGMPGLGRPVSE 498

Query: 634 GGLGFDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSIS 693
           GG+GFDY + ++  + W  +L+N  D++WSM ++ SSL  NR Y  K + YAE+H QSI 
Sbjct: 499 GGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSL-TNRRYTEKCIAYAESHDQSIV 558

Query: 694 GGRSYAEILFGDTKEHG----SGSKETLLRGCSLHKR----------------------- 753
           G ++ A +L       G    + +   + RG +LHK                        
Sbjct: 559 GDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYLNFMGNEFGH 618

Query: 754 ---VEFPMPSNNLSFSLANRQWDLLEKEMHRDLFV--FDKDLMGLDENEKLRTRSLPNVH 813
              ++FP   NN S+    RQW+L + E  R  F+  FD+ +  LDE           V 
Sbjct: 619 PEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKFSFLASGKQIVS 678

Query: 814 HVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKH 826
            +++  KV+ + RG  +F+ NFHP ++YE Y VG +  G+YR+ L++D  ++GG G   H
Sbjct: 679 SMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDAWEFGGHGRTGH 738

BLAST of Carg12832 vs. ExPASy Swiss-Prot
Match: Q6T308 (1,4-alpha-glucan-branching enzyme OS=Felis catus OX=9685 GN=GBE1 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.3e-112
Identity = 214/534 (40.07%), Postives = 313/534 (58.61%), Query Frame = 0

Query: 336 IPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNV 395
           +PHGSK +V   +  G  L RI  WA YV  + E       HW+  PE+ Y +K++RP  
Sbjct: 133 VPHGSKLKVVIRSKSGEILYRISPWAKYVTREGENVNYDWTHWD--PEHPYKFKHSRPKK 192

Query: 396 PKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRV 455
           P+ ++IYE HVGIS  E K++S+ +F   VLP +K+ GYN IQ+  ++EH  Y + GY++
Sbjct: 193 PRGVRIYESHVGISSYEGKIASYKHFTYNVLPRIKDLGYNCIQMMAIMEHAYYASFGYQI 252

Query: 456 TNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHT 515
           T+FFA SSRYGTPE+ K LVD AH +G+ V LD+VHS+A+ +   GL+ FDG++ CYFH+
Sbjct: 253 TSFFAASSRYGTPEELKELVDTAHSMGITVLLDVVHSHASKNSEDGLNMFDGTDSCYFHS 312

Query: 516 GKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFA-SF 575
           G RG+H  W +R+F Y   +V          W+ EY  DGFRF  ++SM+Y H+G   +F
Sbjct: 313 GPRGNHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYGFDGFRFDGVTSMLYHHHGMGQAF 372

Query: 576 TGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDY 635
           +GD  E+    VD+DAL+YLMLAN ++H L+PN ITIAED +  P LC P SQGG+GFDY
Sbjct: 373 SGDYHEYFGLQVDEDALIYLMLANHLVHTLYPNSITIAEDVSGMPALCSPISQGGVGFDY 432

Query: 636 YVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAE 695
            + ++  + W   L+   D++W+M  IV +L  NR Y+ K + YAE+H Q++ G ++ A 
Sbjct: 433 RLAMAIPDKWIQLLKEFKDEDWNMGNIVYTL-TNRRYLEKCIAYAESHDQALVGDKTLAF 492

Query: 696 ILFGDTKEHGSGSKET-----LLRGCSLHKRV--------------------------EF 755
            L  D + + + S  T     + RG  LHK +                          +F
Sbjct: 493 WLM-DAEMYTNMSVLTPFTPVIDRGIQLHKMIRLITHALGGEGYLNFMGNEFGHPEWLDF 552

Query: 756 PMPSNNLSFSLANRQWDLLEKEMHRDLFV--FDKDLMGLDENEKLRTRSLPNVHHVNEAT 815
           P   NN S+  A RQ+ L + ++ R  F+  FD+D+  L+E     +     V   +E  
Sbjct: 553 PRKGNNESYHYARRQFHLTDDDLLRYKFLNNFDRDMNKLEERCGWLSAPQAFVSEKHEGN 612

Query: 816 KVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKH 825
           K+I++ R   +FI NFHP+ SY  Y VG    G++RI+L+TD  +YGG   + H
Sbjct: 613 KIIAFERAGLVFIFNFHPSKSYTDYRVGTTLPGKFRIVLDTDAAEYGGHQRLDH 662

BLAST of Carg12832 vs. ExPASy Swiss-Prot
Match: Q9D6Y9 (1,4-alpha-glucan-branching enzyme OS=Mus musculus OX=10090 GN=Gbe1 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.7e-112
Identity = 224/568 (39.44%), Postives = 322/568 (56.69%), Query Frame = 0

Query: 333 NPTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTR 392
           +P IPHGSK +V   +  G  L RI  WA YV  +        IHW   PE  Y +K++R
Sbjct: 133 SPLIPHGSKLKVVITSKSGEILYRISPWAKYVVRENNNVNYDWIHW--APEDPYKFKHSR 192

Query: 393 PNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 452
           P  P++L+IYE HVGIS  E K++S+ +F   VLP +K+ GYN IQL  ++EH  Y + G
Sbjct: 193 PKKPRSLRIYESHVGISSHEGKIASYKHFTSNVLPRIKDLGYNCIQLMAIMEHAYYASFG 252

Query: 453 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCY 512
           Y++T+FFA SSRYGTPE+ K LVD AH +G+ V LD+VHS+A+ +   GL+ FDG++ CY
Sbjct: 253 YQITSFFAASSRYGTPEELKELVDTAHSMGIVVLLDVVHSHASKNSEDGLNMFDGTDSCY 312

Query: 513 FHTGKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFA 572
           FH+G RG H  W +R+F Y   +V          W+ EY  DGFRF  ++SM+Y H+G  
Sbjct: 313 FHSGPRGTHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYCFDGFRFDGVTSMLYHHHGMG 372

Query: 573 -SFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 632
             F+GD  E+    VD+DAL+YLMLAN + H L+P+ ITIAED +  P LC PTSQGG G
Sbjct: 373 QGFSGDYNEYFGLQVDEDALIYLMLANHLAHTLYPDSITIAEDVSGMPALCSPTSQGGGG 432

Query: 633 FDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 692
           FDY + ++  + W   L+   D++W+M  IV +L  NR Y+ K + YAE+H Q++ G ++
Sbjct: 433 FDYRLAMAIPDKWIQLLKEFKDEDWNMGNIVYTL-TNRRYLEKCVAYAESHDQALVGDKT 492

Query: 693 YAEILFGDTKEHGSGS-----KETLLRGCSLHKRV------------------------- 752
            A  L  D + + + S        + RG  LHK +                         
Sbjct: 493 LAFWLM-DAEMYTNMSVLAPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEW 552

Query: 753 -EFPMPSNNLSFSLANRQWDLLEKEMHRDLFV--FDKDLMGLDENEKLRTRSLPNVHHVN 812
            +FP   NN S+  A RQ++L + ++ R  F+  FD+D+  L+E     +     V   +
Sbjct: 553 LDFPRKGNNESYHYARRQFNLTDDDLLRYKFLNNFDRDMNRLEERCGWLSAPQAYVSEKH 612

Query: 813 EATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHD-Q 855
           EA K I++ R   LFI NFHP+ SY  Y VG    G+++I+L++D  +YGG   + H+  
Sbjct: 613 EANKTITFERAGLLFIFNFHPSKSYTDYRVGTATPGKFKIVLDSDAAEYGGHQRLDHNTN 672

BLAST of Carg12832 vs. ExPASy TrEMBL
Match: A0A6J1FAQ6 (1,4-alpha-glucan branching enzyme OS=Cucurbita moschata OX=3662 GN=LOC111443613 PE=3 SV=1)

HSP 1 Score: 1723.4 bits (4462), Expect = 0.0e+00
Identity = 839/909 (92.30%), Postives = 845/909 (92.96%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+I+CSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EP+VSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPEVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL VFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWDLLEKE
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. ExPASy TrEMBL
Match: A0A6J1IP54 (1,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111476992 PE=3 SV=1)

HSP 1 Score: 1699.9 bits (4401), Expect = 0.0e+00
Identity = 827/909 (90.98%), Postives = 837/909 (92.08%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQ 60
           MNQHSFP MNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKL+IICSASEHSQ
Sbjct: 1   MNQHSFPPMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK--------- 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQ AHFLRER   +K         
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQLAHFLRERYKSLKDLKDEIYDR 120

Query: 121 -FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180
              +    S    LGMHRHVEHRVDFMEWAPGARYCAVIGDFN WSPRE+VAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNGWSPREHVAREGHLGHD 180

Query: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFK KKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKAKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL V LDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVLLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDV          WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKE 780
           LRGCSLH                          KRVEFPMPSNNLSFSLANRQWDLLE++
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEEK 780

Query: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840
           MHRDLFVFDKDLMGL ENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 MHRDLFVFDKDLMGLGENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 864
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHD YLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDHYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Carg12832 vs. ExPASy TrEMBL
Match: A0A1S3BIP3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 1570.8 bits (4066), Expect = 0.0e+00
Identity = 771/913 (84.45%), Postives = 802/913 (87.84%), Query Frame = 0

Query: 1   MNQHSFPAMNAL--SSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEH 60
           MN    P MNAL  S SPS+KF  YKT H LLLP  RSTLQSI+LS KLKLKI CSASEH
Sbjct: 1   MNHQHSPTMNALSHSPSPSIKFSSYKT-HSLLLPQPRSTLQSISLSGKLKLKISCSASEH 60

Query: 61  SQHAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK------- 120
            +HA P K+Q SKK K+VSDGEKGVDPVGFLTKLGISHKQF+ +LRER   +K       
Sbjct: 61  PEHAAPRKHQPSKKAKNVSDGEKGVDPVGFLTKLGISHKQFSQYLRERYKSLKDLKEEIF 120

Query: 121 ---FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLG 180
                +    S    LGMHRHVEHRVDFMEWAPGARYCAV+GDFN WSP EN AREGH G
Sbjct: 121 NRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSPTENAAREGHFG 180

Query: 181 HDDYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGE 240
           HDDYGYWFIILED+LKEGEKP+ELYFQQYNYVDDYDKGDSG+SIDEIFKRANDEYWEPGE
Sbjct: 181 HDDYGYWFIILEDKLKEGEKPDELYFQQYNYVDDYDKGDSGVSIDEIFKRANDEYWEPGE 240

Query: 241 DRFIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDV 300
           DRFIKNRFEVPAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SYDV
Sbjct: 241 DRFIKNRFEVPAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSNLPSYDV 300

Query: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYF 360
           IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKY+PTIPHGSKYRVYF
Sbjct: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYSPTIPHGSKYRVYF 360

Query: 361 NTPDGPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVG 420
           NTP+GPLERIPAWATYVQPDA GKQGFAIHWEPPPEYAY WKNT+PNVPKALKIYECHVG
Sbjct: 361 NTPNGPLERIPAWATYVQPDAHGKQGFAIHWEPPPEYAYKWKNTKPNVPKALKIYECHVG 420

Query: 421 ISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGT 480
           ISG EPKVSSF+YF EKVLPHVKEAGYNAIQLFGVVEHKDYFT+GYRVTNFFAVSSRYGT
Sbjct: 421 ISGSEPKVSSFNYFIEKVLPHVKEAGYNAIQLFGVVEHKDYFTVGYRVTNFFAVSSRYGT 480

Query: 481 PEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540
           PEDFKRLVDEAHGLGL VFLDIVHSYA+ADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR
Sbjct: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYASADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540

Query: 541 MFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600
           MFKYGDQDV          WVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVD
Sbjct: 541 MFKYGDQDVLHFLLSNLNWWVVEYHIDGFLFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600

Query: 601 KDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSF 660
           KDALLYL+LANEILH LHP+IITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMWSSF
Sbjct: 601 KDALLYLILANEILHALHPDIITIAEDATYYPGLCEPISQGGLGFDYYVNLSASEMWSSF 660

Query: 661 LQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGS 720
           LQNVPDQEW+MNKIVSSLI NRH  NKMLL+AENHSQSISGGRSYAEILFGD KEHG+GS
Sbjct: 661 LQNVPDQEWNMNKIVSSLIGNRHSTNKMLLFAENHSQSISGGRSYAEILFGDIKEHGAGS 720

Query: 721 KETLLRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDL 780
           KETLLRGCSLH                          KRVEFPM SNN SFSLANRQWDL
Sbjct: 721 KETLLRGCSLHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDL 780

Query: 781 LEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTD 840
           LEKEMH  LF+FDK+LMGLDENEK+ TRSLPNVHHVNE TKVISYIRGPFLFI NFHPTD
Sbjct: 781 LEKEMHHGLFLFDKELMGLDENEKILTRSLPNVHHVNETTKVISYIRGPFLFIYNFHPTD 840

Query: 841 SYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCR 864
           S+ERYSVGVEEAGEYRI+LNTDE +YGGQG IKHDQYLQRTISRRIDGLRNCLEV LPCR
Sbjct: 841 SFERYSVGVEEAGEYRIMLNTDEIEYGGQGNIKHDQYLQRTISRRIDGLRNCLEVSLPCR 900

BLAST of Carg12832 vs. ExPASy TrEMBL
Match: A0A6J1CCI0 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111010409 PE=3 SV=1)

HSP 1 Score: 1564.7 bits (4050), Expect = 0.0e+00
Identity = 766/903 (84.83%), Postives = 798/903 (88.37%), Query Frame = 0

Query: 9   MNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQ 68
           MN +SS+PS KF PYK+N  LLL HSRST QSIALS KLKL IICSASEH QHAEPAK+Q
Sbjct: 1   MNTISSTPSTKFYPYKSN-FLLLTHSRSTPQSIALSGKLKLNIICSASEHPQHAEPAKHQ 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK----------FTIVMRI 128
            S+K KSVSDGEK VDPVGFLTKLGISHKQFA +LRER   +K            +    
Sbjct: 61  QSRKPKSVSDGEKRVDPVGFLTKLGISHKQFAQYLRERYKSLKDLKDEIFNRHANLQDLS 120

Query: 129 SRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           S    LGMHRHVEHRVDFMEWAPGARYCAV+GDFN WS  EN AREGHLGHDDYGYWFII
Sbjct: 121 SGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSVTENAAREGHLGHDDYGYWFII 180

Query: 189 LEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LED+LK+GEKPEELYFQQYNYVDDYDKGDSGI+IDEIFKRANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLKDGEKPEELYFQQYNYVDDYDKGDSGITIDEIFKRANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVIDNGKEYDIF 308
           PAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SY VID+ KEYDIF
Sbjct: 241 PAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSDLPSYHVIDSEKEYDIF 300

Query: 309 NVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERI 368
           NVVIDP TREKFKVKKPPI YWLETRKGRKAWLKKY+PTIPHGSKYRVYFNTP+GPLERI
Sbjct: 301 NVVIDPKTREKFKVKKPPIAYWLETRKGRKAWLKKYSPTIPHGSKYRVYFNTPNGPLERI 360

Query: 369 PAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKVSS 428
           PAWATYVQPDA+GKQ FAIHWEPPPEYAY WKN++P+VPKALKIYECHVGISG EPKVSS
Sbjct: 361 PAWATYVQPDADGKQAFAIHWEPPPEYAYKWKNSKPSVPKALKIYECHVGISGSEPKVSS 420

Query: 429 FDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDE 488
           F+YF EKVLPHVKEAGYNAIQL G+VEHKDYFT+GYRVTNFFAVSSRYGTPEDFKRLVDE
Sbjct: 421 FNYFIEKVLPHVKEAGYNAIQLIGLVEHKDYFTVGYRVTNFFAVSSRYGTPEDFKRLVDE 480

Query: 489 AHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDV- 548
           AHGLGL VFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDV 
Sbjct: 481 AHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVL 540

Query: 549 ---------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLA 608
                    WVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYL+LA
Sbjct: 541 HFLLSNLNWWVVEYGIDGFHFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLILA 600

Query: 609 NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQEWS 668
           NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFL+NVPD EWS
Sbjct: 601 NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLENVPDHEWS 660

Query: 669 MNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGCSL 728
           MNKIVSSL DNRHY NKMLLYAENH+QSISGGRSYAEILFGD KEHG GSKETLLRGCSL
Sbjct: 661 MNKIVSSLTDNRHYANKMLLYAENHNQSISGGRSYAEILFGDIKEHGPGSKETLLRGCSL 720

Query: 729 H--------------------------KRVEFPMPSNNLSFSLANRQWDLLEKEMHRDLF 788
           H                          KRVEFPMPSNN SFSLANRQWDLLEKE+HRDLF
Sbjct: 721 HKMIRTITFTIGGCAYLNFMGNEFGHPKRVEFPMPSNNFSFSLANRQWDLLEKEVHRDLF 780

Query: 789 VFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVE 848
           VFDKDLMGLDENE+L TRSLPN+HH+NE TKVISYIRGPFLFI NFHPTDSYERYSVGVE
Sbjct: 781 VFDKDLMGLDENERLLTRSLPNIHHINETTKVISYIRGPFLFIFNFHPTDSYERYSVGVE 840

Query: 849 EAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLSRI 864
           EAGEYRIILNTDE KYGG+GIIKHD YLQRTIS+RIDGLRNCLEV LP RTAQVYKLSRI
Sbjct: 841 EAGEYRIILNTDEIKYGGRGIIKHDHYLQRTISQRIDGLRNCLEVSLPSRTAQVYKLSRI 900

BLAST of Carg12832 vs. ExPASy TrEMBL
Match: A0A1S4DX00 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 1553.1 bits (4020), Expect = 0.0e+00
Identity = 761/903 (84.27%), Postives = 792/903 (87.71%), Query Frame = 0

Query: 1   MNQHSFPAMNAL--SSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEH 60
           MN    P MNAL  S SPS+KF  YKT H LLLP  RSTLQSI+LS KLKLKI CSASEH
Sbjct: 1   MNHQHSPTMNALSHSPSPSIKFSSYKT-HSLLLPQPRSTLQSISLSGKLKLKISCSASEH 60

Query: 61  SQHAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK------- 120
            +HA P K+Q SKK K+VSDGEKGVDPVGFLTKLGISHKQF+ +LRER   +K       
Sbjct: 61  PEHAAPRKHQPSKKAKNVSDGEKGVDPVGFLTKLGISHKQFSQYLRERYKSLKDLKEEIF 120

Query: 121 ---FTIVMRISRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLG 180
                +    S    LGMHRHVEHRVDFMEWAPGARYCAV+GDFN WSP EN AREGH G
Sbjct: 121 NRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSPTENAAREGHFG 180

Query: 181 HDDYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGE 240
           HDDYGYWFIILED+LKEGEKP+ELYFQQYNYVDDYDKGDSG+SIDEIFKRANDEYWEPGE
Sbjct: 181 HDDYGYWFIILEDKLKEGEKPDELYFQQYNYVDDYDKGDSGVSIDEIFKRANDEYWEPGE 240

Query: 241 DRFIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDV 300
           DRFIKNRFEVPAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SYDV
Sbjct: 241 DRFIKNRFEVPAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSNLPSYDV 300

Query: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYF 360
           IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKY+PTIPHGSKYRVYF
Sbjct: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYSPTIPHGSKYRVYF 360

Query: 361 NTPDGPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVG 420
           NTP+GPLERIPAWATYVQPDA GKQGFAIHWEPPPEYAY WKNT+PNVPKALKIYECHVG
Sbjct: 361 NTPNGPLERIPAWATYVQPDAHGKQGFAIHWEPPPEYAYKWKNTKPNVPKALKIYECHVG 420

Query: 421 ISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGT 480
           ISG EPKVSSF+YF EKVLPHVKEAGYNAIQLFGVVEHKDYFT+GYRVTNFFAVSSRYGT
Sbjct: 421 ISGSEPKVSSFNYFIEKVLPHVKEAGYNAIQLFGVVEHKDYFTVGYRVTNFFAVSSRYGT 480

Query: 481 PEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540
           PEDFKRLVDEAHGLGL VFLDIVHSYA+ADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR
Sbjct: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYASADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540

Query: 541 MFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600
           MFKYGDQDV          WVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVD
Sbjct: 541 MFKYGDQDVLHFLLSNLNWWVVEYHIDGFLFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600

Query: 601 KDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSF 660
           KDALLYL+LANEILH LHP+IITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMWSSF
Sbjct: 601 KDALLYLILANEILHALHPDIITIAEDATYYPGLCEPISQGGLGFDYYVNLSASEMWSSF 660

Query: 661 LQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGS 720
           LQNVPDQEW+MNKIVSSLI NRH  NKMLL+AENHSQSISGGRSYAEILFGD KEHG+GS
Sbjct: 661 LQNVPDQEWNMNKIVSSLIGNRHSTNKMLLFAENHSQSISGGRSYAEILFGDIKEHGAGS 720

Query: 721 KETLLRGCSLH--------------------------KRVEFPMPSNNLSFSLANRQWDL 780
           KETLLRGCSLH                          KRVEFPM SNN SFSLANRQWDL
Sbjct: 721 KETLLRGCSLHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDL 780

Query: 781 LEKEMHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTD 840
           LEKEMH  LF+FDK+LMGLDENEK+ TRSLPNVHHVNE TKVISYIRGPFLFI NFHPTD
Sbjct: 781 LEKEMHHGLFLFDKELMGLDENEKILTRSLPNVHHVNETTKVISYIRGPFLFIYNFHPTD 840

Query: 841 SYERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCR 854
           S+ERYSVGVEEAGEYRI+LNTDE +YGGQG IKHDQYLQRTISRRIDGLRNCLEV LPCR
Sbjct: 841 SFERYSVGVEEAGEYRIMLNTDEIEYGGQGNIKHDQYLQRTISRRIDGLRNCLEVSLPCR 900

BLAST of Carg12832 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 614/905 (67.85%), Postives = 708/905 (78.23%), Query Frame = 0

Query: 9   MNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KLKI C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK----------FTIVMRI 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER   +K          F      
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           S   LLGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LED+L+EGE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AY WK ++P VP++L+IYECHVGISG EPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGL VFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 V----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           V          W+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHK--------------------------RVEFPMPSNNLSFSLANRQWDLLEKEMHRD 788
           SLHK                          RVEFP  SNN SFSLANR+WDLLE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVG 848
           LF FDK+LM LD+++ + +R LP++HHVN+A  VIS+ RGPFLFI NFHP++SYE+Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 864
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 899

BLAST of Carg12832 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 607/912 (66.56%), Postives = 701/912 (76.86%), Query Frame = 0

Query: 9   MNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KLKI C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK----------FTIVMRI 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER   +K          F      
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           S   LLGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LED+L+EGE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQP-----DAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISG 428
           R+PAWATYVQP     + EGKQ +AIHWEP PE AY WK ++P VP++L+IYECHVGISG
Sbjct: 361 RVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISG 420

Query: 429 PEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPED 488
            EPKVS+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+D
Sbjct: 421 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDD 480

Query: 489 FKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFK 548
           FKRLVDEAHGLGL VFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFK
Sbjct: 481 FKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFK 540

Query: 549 YGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDA 608
           YGD DV          W+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DA
Sbjct: 541 YGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDA 600

Query: 609 LLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQN 668
           L+YL+LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L N
Sbjct: 601 LMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDN 660

Query: 669 VPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKET 728
           VPD EWSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE 
Sbjct: 661 VPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKEL 720

Query: 729 LLRGCSLHK--------------------------RVEFPMPSNNLSFSLANRQWDLLEK 788
           L RG SLHK                          RVEFP  SNN SFSLANR+WDLLE 
Sbjct: 721 LDRGISLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLES 780

Query: 789 EMHRDLFVFDK--DLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDS 848
            +H  LF FDK  +LM LD+++ + +R LP++HHVN+A  VIS+ RGPFLFI NFHP++S
Sbjct: 781 GVHHHLFSFDKVSELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNS 840

Query: 849 YERYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRT 864
           YE+Y VGVEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLE       
Sbjct: 841 YEKYDVGVEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLE------- 897

BLAST of Carg12832 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 588/905 (64.97%), Postives = 681/905 (75.25%), Query Frame = 0

Query: 9   MNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLKIICSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KLKI C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERTSRMK----------FTIVMRI 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER   +K          F      
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SRTCLLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           S   LLGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LED+L+EGE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGPEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AY WK ++P VP++L+IYECHVGISG EPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +K                              VTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKK------------------------------VTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGL VFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 V----------WVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           V          W+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDQE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHK--------------------------RVEFPMPSNNLSFSLANRQWDLLEKEMHRD 788
           SLHK                          RVEFP  SNN SFSLANR+WDLLE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVG 848
           LF FDK+LM LD+++ + +R LP++HHVN+A  VIS+ RGPFLFI NFHP++SYE+Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 864
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 869

BLAST of Carg12832 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 393.7 bits (1010), Expect = 3.9e-109
Identity = 220/586 (37.54%), Postives = 330/586 (56.31%), Query Frame = 0

Query: 333 NPTIPHGSKYRVYFNTPDGPLERIPAWATYVQPDAEGKQGF-AIHWEPPPEYAYNWKNTR 392
           +P IPHGS+ ++  +TP G  + IPAW  +    A G+  F  I+++PP E  Y +K+ +
Sbjct: 226 SPAIPHGSRVKIRMDTPSGIKDSIPAWIKF-SVQAPGEIPFNGIYYDPPEEEKYVFKHPQ 285

Query: 393 PNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 452
           P  PK+L+IYE HVG+S  EP V+++  FR+ VLP +K+ GYNA+Q+  + EH  Y + G
Sbjct: 286 PKRPKSLRIYEAHVGMSSTEPMVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFG 345

Query: 453 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCY 512
           Y VTNFFA SSR GTPE+ K L+D AH LGL V +DIVHS+A+ + + GL+ FDG++  Y
Sbjct: 346 YHVTNFFAPSSRCGTPEELKSLIDRAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDAHY 405

Query: 513 FHTGKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGFA 572
           FH+G RG+H  W +R+F YG  +V          W+ EY+ DGFRF  ++SM+YTH+G +
Sbjct: 406 FHSGPRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLS 465

Query: 573 -SFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 632
             FTG+  E+     D DA+ YLML N+++HGL+P  IT+ ED +  P  C P   GG+G
Sbjct: 466 VGFTGNYTEYFGLETDVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVG 525

Query: 633 FDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 692
           FDY ++++ ++ W   L+   D++W M  I+ +L  NR +  K + YAE+H Q++ G ++
Sbjct: 526 FDYRLHMAIADKWIEMLKK-RDEDWQMGDIIYTL-TNRRWSEKCISYAESHDQALVGDKT 585

Query: 693 YAEILFG----DTKEHGSGSKETLLRGCSLHKR--------------------------V 752
            A  L      D       S   + RG +LHK                           +
Sbjct: 586 IAFWLMDKDMYDFMAVDRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 645

Query: 753 EFP-----------MPSNNLSFSLANRQWDLLEKE--MHRDLFVFDKDLMGLDENEKLRT 812
           +FP           +P NN S+    R++DL + +   +R L  FD+ +  L+EN    T
Sbjct: 646 DFPRGEQRLSDGSVIPGNNFSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMT 705

Query: 813 RSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYG 859
                +   +EA +VI + RG  +F+ NFH T SY  Y +G  + G+Y+I+L++D+  +G
Sbjct: 706 SEHQFISRKDEADRVIVFERGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFG 765


HSP 2 Score: 43.9 bits (102), Expect = 7.6e-04
Identity = 16/46 (34.78%), Postives = 29/46 (63.04%), Query Frame = 0

Query: 134 VDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIIL 180
           + + EWAPGA+  ++IGDFN+W+   ++     +  +++G W I L
Sbjct: 179 ITYREWAPGAKAASLIGDFNNWNSNADI-----MTRNEFGVWEIFL 219

BLAST of Carg12832 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 392.1 bits (1006), Expect = 1.1e-108
Identity = 213/579 (36.79%), Postives = 321/579 (55.44%), Query Frame = 0

Query: 333 NPTIPHGSKYRVYFNTPDGPLERIPAWATY-VQPDAEGKQGFAIHWEPPPEYAYNWKNTR 392
           +P IPHGS+ ++  +TP G  + IPAW  Y VQP  E      ++++PP E  Y +K+ R
Sbjct: 261 SPAIPHGSRVKIRMDTPSGIKDSIPAWIKYSVQPPGEIPYN-GVYYDPPEEDKYAFKHPR 320

Query: 393 PNVPKALKIYECHVGISGPEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 452
           P  P +L+IYE HVG+S  EPK++++  FR+ VLP +K+ GYNA+Q+  + EH  Y + G
Sbjct: 321 PKKPTSLRIYESHVGMSSTEPKINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFG 380

Query: 453 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCY 512
           Y VTNFFA SSR+GTP+D K L+D+AH LGL V +DIVHS+A+ + + GL  FDG++  Y
Sbjct: 381 YHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLDMFDGTDGQY 440

Query: 513 FHTGKRGHHKYWGTRMFKYGDQDV----------WVVEYRIDGFRFHSLSSMIYTHNGF- 572
           FH+G RG+H  W +R+F YG  +V          W+ EY+ DGFRF  ++SM+YTH+G  
Sbjct: 441 FHSGSRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQ 500

Query: 573 ASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 632
             FTG+  E+     D DA++YLML N+++HGL+P  I + ED +  P  C P   GG+G
Sbjct: 501 VEFTGNYNEYFGYSTDVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVG 560

Query: 633 FDYYVNLSASEMWSSFLQNVPDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 692
           FDY ++++ ++ W   L+   D++W +  I  +L  NR +  K ++YAE+H Q++ G ++
Sbjct: 561 FDYRLHMAVADKWIELLKK-RDEDWQVGDITFTL-TNRRWGEKCVVYAESHDQALVGDKT 620

Query: 693 YAEILFG----DTKEHGSGSKETLLRGCSLHKR--------------------------V 752
            A  L      D       +   + RG +LHK                           +
Sbjct: 621 IAFWLMDKDMYDFMAVDRQATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 680

Query: 753 EFP-----------MPSNNLSFSLANRQWDLLEKE--MHRDLFVFDKDLMGLDENEKLRT 812
           +FP           +  NN S+    R++DL + E   +  L  FD+ +  L+E     T
Sbjct: 681 DFPRTDQHLPDGRVIAGNNGSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMT 740

Query: 813 RSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYERYSVGVEEAGEYRIILNTDETKYG 856
                +   +E  +VI + RG  LF+ NFH T+SY  Y +G    G+Y+I+L++D + +G
Sbjct: 741 SEHQYISRKDEGDRVIVFERGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFG 800


HSP 2 Score: 47.8 bits (112), Expect = 5.3e-05
Identity = 18/46 (39.13%), Postives = 30/46 (65.22%), Query Frame = 0

Query: 134 VDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHDDYGYWFIIL 180
           + + EWAPGA+  ++IGDFN+W+ + +V     +  +D+G W I L
Sbjct: 214 ITYREWAPGAKAASLIGDFNNWNAKSDV-----MARNDFGVWEIFL 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024931.10.0e+00100.001,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic, partial [Cucurb... [more]
KAG6592053.10.0e+0093.181,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic, partial [Cucurb... [more]
XP_022937279.10.0e+0092.301,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita mosch... [more]
XP_023535701.10.0e+0091.531,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo ... [more]
XP_022976653.10.0e+0090.981,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
D2WL320.0e+0067.851,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic OS=Arabidopsis t... [more]
Q8NKE18.5e-11736.701,4-alpha-glucan-branching enzyme OS=Rhizophagus irregularis (strain DAOM 181602... [more]
P309246.7e-11439.371,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2[more]
Q6T3081.3e-11240.071,4-alpha-glucan-branching enzyme OS=Felis catus OX=9685 GN=GBE1 PE=2 SV=1[more]
Q9D6Y91.7e-11239.441,4-alpha-glucan-branching enzyme OS=Mus musculus OX=10090 GN=Gbe1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FAQ60.0e+0092.301,4-alpha-glucan branching enzyme OS=Cucurbita moschata OX=3662 GN=LOC111443613 ... [more]
A0A6J1IP540.0e+0090.981,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111476992 PE... [more]
A0A1S3BIP30.0e+0084.451,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 S... [more]
A0A6J1CCI00.0e+0084.831,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111010409... [more]
A0A1S4DX000.0e+0084.271,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G20440.20.0e+0067.85Alpha amylase family protein [more]
AT3G20440.30.0e+0066.56Alpha amylase family protein [more]
AT3G20440.10.0e+0064.97Alpha amylase family protein [more]
AT5G03650.13.9e-10937.54starch branching enzyme 2.2 [more]
AT2G36390.11.1e-10836.79starch branching enzyme 2.1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0374391,4-alpha-glucan-branching enzymePIRSFPIRSF000463GlgBcoord: 18..863
e-value: 1.2E-186
score: 620.2
IPR0374391,4-alpha-glucan-branching enzymePANTHERPTHR436511,4-ALPHA-GLUCAN-BRANCHING ENZYMEcoord: 33..854
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 425..514
e-value: 1.8E-10
score: 40.9
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 124..186
e-value: 2.2E-7
score: 31.1
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 122..228
e-value: 1.6E-16
score: 62.8
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 331..384
e-value: 6.7E-127
score: 426.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 385..708
e-value: 6.7E-127
score: 426.2
NoneNo IPR availablePANTHERPTHR43651:SF41,4-ALPHA-GLUCAN-BRANCHING ENZYME 3, CHLOROPLASTIC/AMYLOPLASTICcoord: 33..854
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 763..857
IPR006048Alpha-amylase/branching enzyme, C-terminal all betaPFAMPF02806Alpha-amylase_Ccoord: 765..857
e-value: 2.6E-21
score: 75.9
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 761..860
e-value: 3.0E-27
score: 96.7
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 337..680
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 123..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12832-RACarg12832-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005978 glycogen biosynthetic process
biological_process GO:0009791 post-embryonic development
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009501 amyloplast
cellular_component GO:0009507 chloroplast
molecular_function GO:0003844 1,4-alpha-glucan branching enzyme activity
molecular_function GO:0102752 1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)
molecular_function GO:0043169 cation binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003824 catalytic activity