Carg12770 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12770
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionvesicle-associated protein 4-1-like
LocationCarg_Chr09: 4949692 .. 4953784 (-)
RNA-Seq ExpressionCarg12770
SyntenyCarg12770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCGCGTGTAAATCAAGTACCCTAAAAGCGCGTGGGATAATTATTCCAAACAATTCCATCTCATCTGAAAAAAAATCTTACAACTCAGAGCAGCACGGATTCGTAATAATTGCACGAACGGCCATTGGGCTGTCAAGACGTATTTTCCGTGTGACACGTGTCCACCCGCACGGACATCAAAACCATATTCCGCCGTATTTACATCCGCCTCTCGAATCCGCTCTCCGCCACCGTCGCCTCCATCAGAGAGGAATCGGCGATTTTCGGTTTGGCCCGGGAAACCTTAATGTGATAGTGTTAATGACCATGACTGTCGCCGACCGTCACAAGCCTCACGCCGATGTGAAGTTCTTCAGGCTATGTTCGATTTGGCAATCGGGGAATACTTCGTCTTCTTCGTTGTCGACTCAGAACATTCACCATACTCGAAATGGACCTAAAGATAGAAATTCAACGGCGTCGAATAACGTGAAGGCTTCATCGTCTTCCATGACGGTTTCCTCTGTAGCGAAGTCGCTGTTGCCTCCTCGGCGTAGGCTCCACCTTGATCCTAACAATTCTTTATTCTTTCCTTGTAAGTTCTTGTTCTCTAATTTTTCTCTTTTCCGTTGCTGGTGTTTAGTTTTCTGAATCGAATTGGAATCTCTCCTTGTTTTTGAGTTGTATTGTTTGAAATTTGAATTGGAAATTGGATGTTTTCTTCAATCTGGATAATGCGAGCCAAAACTATGCTCGTTTGCTCTTGGATTAGAACATTTTACTTCCTTGAATTGAATCAAATAATCGTTCTAGCCTTCGGTTTCTCCTGCTACCATTTGCGGTCAGTTCATATTTACTTGGAGAGCAGGGGAATTACAAGTTTTGGAAAATTGTACGTAATTATTGTTCAGAATTTGCGTTCATCCTGAACTGTGTATTGTTTGATGGATTTTCTGTAGATGAACCCGGGAAGCAGGTAAAGAGCGCCATTAGGATGAAGAATACTAGCAGATCCCATGTAGCTTTCAAGGTATCACTTCAATTTCTTCCATTGCAGTCCTTCTTTCGCCTTTTGTTCCTTTAATTATTCTTATATTGATTTCTTACTTTTTAATTCCCAACAAGCCATAATGGAAACATGAATGCATGTGAATCTGTTCTTCTGATGTTCTTTTTGTTCTTTACTTTGAGACGATGACTGTTATCATGCTGATCTTCCATTACCATTTTCTGAAACTCCATGCATCTTAGTAATTGTTCATCTTCTGGGTAATGAATGTTGTTGGGAACTTTATTAAACGATAGTTTCTGGTTTCAGCCATCTAACAATTGCTTGTTTCTTAGCATAGTTAGTCCATGGGGTCTATTTGATTTCCTCAATCAGTGTAAATTCTGCATTCAAATTGTTTTCAGCTCAGGAAATCAAGTTTTTCGACCTTTTAATTCGTTAACGACGAGGATTTTGTATCAAATTTTTCAAAGAGTTATTGTGAGATCCCACATCGGTTGGAGATGGAAACGAAACATTTTTTATAAGGGTGTAGAAACTTCTCCTTAGCATACACGTTTTAAAAACCTTGAGGGGAAGCCTAGAAGGAAAAGTTTAAAGATGACGATATTTTCTAGCGATGGGTTTGGACTTGGGCTATTACAAATGGTATTAGAGCCCGACACCAGTTGGTGTGTGAGCAAGGACGCTAGGCCCCAAGTGAGGTGGATTGTGAGACAACGAATGCCAGCGAGGACGCTAAGCCTCGAAGGGGGGTCGATAGTGAGATCCCACATCGGTTGGAGATATTCTTTATAAGAGTGTAGAAATCTTCTTAGTAGACACGTTTTTAAACCTTGAGAGGAAACCTAGAAGGGAAAGCCCAAAGAGGACAATATTGGCTAGCGATGAGCTTGGGCTGATATAGTTATTGCAAGTCTCAACTCATTCAACGATTAGATTATTGAAGTCATCGTATTATCAATCTAGGTTTGGTTTTCGTAATGTAAATTGATATCGCCTGAAAGGCAGGTAAAGGCTGATGAATTGCATACTTGTTTGAATGGCATTTCAAGCCGATATGAACTGCTGCTCATTTCTATGATTAATAGCTTAAAATGTGAGTATCTGTACCATTCTAAATGGTCTCTACATCTTTCTTCAGTTTCAAACTACGGCCCCGAAGAGCTGTTATATGCGGCCTCCTGGTGGCATTCTTGCTCCTGAAGAAATTTTTATTGCAACTGGTAATGTTTCCACTGTCATGATCTTTTCTCTTTTTGGTGGTAATGTTAGTGTTTTCAATTCATTAATGTACTTTCATTTCTCATTTTTCATGGTTTTCTAACTTTTGATACTAATGAACATCAACACAAACAAGTATTCATTGTCTTCTTCCATCTTTTTAACAGTCTTCAGATTCGTGGAGCACCCCGAGAACAATGAAAAGCCAAAGGACCATAAGAGCAAAATTAAGTTTAAGATTATGAGCCTTAAAGTGAAAGGTGGAACAGAATACGCACCCGAGCTGGTGAGTCGCTCTATGCTGTTTCGAGCTTTGTTAAACTTAAACCTCTCTGGCTCTCTATTATGAAAGATAAGCCAATGTCTTGATTTCTTTGTTACTTCTTGTGTACTGTTTTGACAGCTTAAGGTTTCCATTGTGCTAACTCAATGCAGTCGATTTTGATTAATGACTTTTTTGTCAAACAGCCTCTCGAGTTTGTGTTGAGGATTATTGGGAGTGAGTCCCATATTGGTTAATTTAGTGGAAGAGCATGGGTTTATAAGTGGGGAATACTATCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCCATGCCCTAAACTTAGCCATGTCAATAGAATCTTCAAATATCGAACAAAGAATTGTCAGCCTCGAAGGTGTAGTCAAAAGTGACTAGAGTGTCGAACAAAGAGTGTACTTTGTTCGAGAGCTCCAGATAACGGAGTCAAGCCTCGATTAAGGGGAGGCTATTCGAGAGCTCCGTAAGTCACAGTGAAGGCTTATAAGTTTGTAGGTTCTATTGTCATCTAATCATTGTACTAGTTTGTTTAATTGTTATTCGAAGTTGTACGAAATCGATTCGGTTCATGTTTGTGTTCGACCACTAAAGGATACATTGTGAAAGTTCGAATTTAGTTGTCTAACTTTTTGATATTGTGTATTTATGTTCTGCAACATTTTCAGTTTGATGAACAGAAGGACCAAGTAATAGTAGAGCGGATTTTGCGGGTCATATTCGTAGATCCAGAGCGACCTAGTGCAGTAAGTAAATCAATGAACACAAGTCTTGTTCTTGGGAGATAGAGGTCCCAATTTAACTGCATTTTGATATTATAGGCTCTCGAAAGACTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTCGAAGCACGCAAGAAGCCTCCAGCAGAATCAGGTCCTCAGGTTGTTGGTGGTGAAGGTCTTGTGATAGATGAATGGGTACGTACGATGATCTTGACCCAACCCAACCCAACCCAACCTGACCGTGCATTTTCGATTCGATATTCATGTTATCAATGTTGAGCTCATCTTATATTCCTATACTGATCTTATAATCCTCTGGATTTCGATTTTGGATCGGACAGAAAGAACGGAGGGAGAAGTACCTCGCTCGTCAACAAGTCGGCGCAGTAGACTCGGTGTTGAGTTAGATTTAACTGTTGCACTACACTCTTTTCAATGGTAAATCTGTACAGGTATTATCATCATCATCTTCAAGCTCTGGATATCTGGAAGATGGAAGGAGTCTTTAGTTGGAAAGTTCGACTGAGCGAAAAATAATCCGATGTCGATGTCATTACTAATGCGAAGAACTTGATTGATCGAAAGTCGAACGCTGCCTATTGTATATAATGAATTTTGGTTGTAAAAATAAATGTACTACTGATTATGATCATAAGATATATATAATGTGTGTGTGGTTTCATGATTGGAATTGGATTATAAATCATTTTGGATCCATTAGATATGTAATATTGTGTTTCCAAAAATTCCATAATTTTGAGGATAATCATTTATTTTTTATTTTTTAAAAATTAAGCTTATAAACGCTCACTT

mRNA sequence

ATGTGCGCGTGTAAATCAAGTACCCTAAAAGCGCGTGGGATAATTATTCCAAACAATTCCATCTCATCTGAAAAAAAATCTTACAACTCAGAGCAGCACGGATTCGTAATAATTGCACGAACGGCCATTGGGCTGTCAAGACGTATTTTCCGTGTGACACGTGTCCACCCGCACGGACATCAAAACCATATTCCGCCGTATTTACATCCGCCTCTCGAATCCGCTCTCCGCCACCGTCGCCTCCATCAGAGAGGAATCGGCGATTTTCGGTTTGGCCCGGGAAACCTTAATGTGATAGTGTTAATGACCATGACTGTCGCCGACCGTCACAAGCCTCACGCCGATGTGAAGTTCTTCAGGCTATGTTCGATTTGGCAATCGGGGAATACTTCGTCTTCTTCGTTGTCGACTCAGAACATTCACCATACTCGAAATGGACCTAAAGATAGAAATTCAACGGCGTCGAATAACGTGAAGGCTTCATCGTCTTCCATGACGGTTTCCTCTGTAGCGAAGTCGCTGTTGCCTCCTCGGCGTAGGCTCCACCTTGATCCTAACAATTCTTTATTCTTTCCTTATGAACCCGGGAAGCAGGTAAAGAGCGCCATTAGGATGAAGAATACTAGCAGATCCCATGTAGCTTTCAAGTTTCAAACTACGGCCCCGAAGAGCTGTTATATGCGGCCTCCTGGTGGCATTCTTGCTCCTGAAGAAATTTTTATTGCAACTGGTAATGTTTCCACTGTCATGATCTTTTCTCTTTTTGGTGTCTTCAGATTCGTGGAGCACCCCGAGAACAATGAAAAGCCAAAGGACCATAAGAGCAAAATTAAGTTTAAGATTATGAGCCTTAAAGTGAAAGGTGGAACAGAATACGCACCCGAGCTGTTTGATGAACAGAAGGACCAAGTAATAGTAGAGCGGATTTTGCGGGTCATATTCGTAGATCCAGAGCGACCTAGTGCAGCTCTCGAAAGACTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTCGAAGCACGCAAGAAGCCTCCAGCAGAATCAGGTCCTCAGGTTGTTGGTGGTGAAGGTCTTGTGATAGATGAATGGAAAGAACGGAGGGAGAAGTACCTCGCTCGTCAACAAGTCGGCGCAGTAGACTCGGTGTTGAGTTAGATTTAACTGTTGCACTACACTCTTTTCAATGGTAAATCTGTACAGGTATTATCATCATCATCTTCAAGCTCTGGATATCTGGAAGATGGAAGGAGTCTTTAGTTGGAAAGTTCGACTGAGCGAAAAATAATCCGATGTCGATGTCATTACTAATGCGAAGAACTTGATTGATCGAAAGTCGAACGCTGCCTATTGTATATAATGAATTTTGGTTGTAAAAATAAATGTACTACTGATTATGATCATAAGATATATATAATGTGTGTGTGGTTTCATGATTGGAATTGGATTATAAATCATTTTGGATCCATTAGATATGTAATATTGTGTTTCCAAAAATTCCATAATTTTGAGGATAATCATTTATTTTTTATTTTTTAAAAATTAAGCTTATAAACGCTCACTT

Coding sequence (CDS)

ATGTGCGCGTGTAAATCAAGTACCCTAAAAGCGCGTGGGATAATTATTCCAAACAATTCCATCTCATCTGAAAAAAAATCTTACAACTCAGAGCAGCACGGATTCGTAATAATTGCACGAACGGCCATTGGGCTGTCAAGACGTATTTTCCGTGTGACACGTGTCCACCCGCACGGACATCAAAACCATATTCCGCCGTATTTACATCCGCCTCTCGAATCCGCTCTCCGCCACCGTCGCCTCCATCAGAGAGGAATCGGCGATTTTCGGTTTGGCCCGGGAAACCTTAATGTGATAGTGTTAATGACCATGACTGTCGCCGACCGTCACAAGCCTCACGCCGATGTGAAGTTCTTCAGGCTATGTTCGATTTGGCAATCGGGGAATACTTCGTCTTCTTCGTTGTCGACTCAGAACATTCACCATACTCGAAATGGACCTAAAGATAGAAATTCAACGGCGTCGAATAACGTGAAGGCTTCATCGTCTTCCATGACGGTTTCCTCTGTAGCGAAGTCGCTGTTGCCTCCTCGGCGTAGGCTCCACCTTGATCCTAACAATTCTTTATTCTTTCCTTATGAACCCGGGAAGCAGGTAAAGAGCGCCATTAGGATGAAGAATACTAGCAGATCCCATGTAGCTTTCAAGTTTCAAACTACGGCCCCGAAGAGCTGTTATATGCGGCCTCCTGGTGGCATTCTTGCTCCTGAAGAAATTTTTATTGCAACTGGTAATGTTTCCACTGTCATGATCTTTTCTCTTTTTGGTGTCTTCAGATTCGTGGAGCACCCCGAGAACAATGAAAAGCCAAAGGACCATAAGAGCAAAATTAAGTTTAAGATTATGAGCCTTAAAGTGAAAGGTGGAACAGAATACGCACCCGAGCTGTTTGATGAACAGAAGGACCAAGTAATAGTAGAGCGGATTTTGCGGGTCATATTCGTAGATCCAGAGCGACCTAGTGCAGCTCTCGAAAGACTGAAACGTCAATTGGCCGAAGCCGAGGCTGCACTCGAAGCACGCAAGAAGCCTCCAGCAGAATCAGGTCCTCAGGTTGTTGGTGGTGAAGGTCTTGTGATAGATGAATGGAAAGAACGGAGGGAGAAGTACCTCGCTCGTCAACAAGTCGGCGCAGTAGACTCGGTGTTGAGTTAG

Protein sequence

MCACKSSTLKARGIIIPNNSISSEKKSYNSEQHGFVIIARTAIGLSRRIFRVTRVHPHGHQNHIPPYLHPPLESALRHRRLHQRGIGDFRFGPGNLNVIVLMTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS
Homology
BLAST of Carg12770 vs. NCBI nr
Match: KAG7024870.1 (Vesicle-associated protein 4-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MCACKSSTLKARGIIIPNNSISSEKKSYNSEQHGFVIIARTAIGLSRRIFRVTRVHPHGH 60
           MCACKSSTLKARGIIIPNNSISSEKKSYNSEQHGFVIIARTAIGLSRRIFRVTRVHPHGH
Sbjct: 1   MCACKSSTLKARGIIIPNNSISSEKKSYNSEQHGFVIIARTAIGLSRRIFRVTRVHPHGH 60

Query: 61  QNHIPPYLHPPLESALRHRRLHQRGIGDFRFGPGNLNVIVLMTMTVADRHKPHADVKFFR 120
           QNHIPPYLHPPLESALRHRRLHQRGIGDFRFGPGNLNVIVLMTMTVADRHKPHADVKFFR
Sbjct: 61  QNHIPPYLHPPLESALRHRRLHQRGIGDFRFGPGNLNVIVLMTMTVADRHKPHADVKFFR 120

Query: 121 LCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRR 180
           LCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRR
Sbjct: 121 LCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRR 180

Query: 181 LHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIF 240
           LHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIF
Sbjct: 181 LHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIF 240

Query: 241 IATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQ 300
           IATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQ
Sbjct: 241 IATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQ 300

Query: 301 KDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVVGGEGLVI 360
           KDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVVGGEGLVI
Sbjct: 301 KDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAESGPQVVGGEGLVI 360

Query: 361 DEWKERREKYLARQQVGAVDSVLS 385
           DEWKERREKYLARQQVGAVDSVLS
Sbjct: 361 DEWKERREKYLARQQVGAVDSVLS 384

BLAST of Carg12770 vs. NCBI nr
Match: XP_022937296.1 (vesicle-associated protein 4-1-like [Cucurbita moschata])

HSP 1 Score: 518.8 bits (1335), Expect = 3.9e-143
Identity = 268/283 (94.70%), Postives = 270/283 (95.41%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKAS 161
           MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLST+NIHHTRNGPKDRNSTASNNVKAS
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTRNIHHTRNGPKDRNSTASNNVKAS 60

Query: 162 SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 221
           SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA
Sbjct: 61  SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 120

Query: 222 PKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKI 281
           PKSCYMRPPGGILAPEEIFIAT             VFRFVEHPENNEKPKDHKSKIKFKI
Sbjct: 121 PKSCYMRPPGGILAPEEIFIAT-------------VFRFVEHPENNEKPKDHKSKIKFKI 180

Query: 282 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 341
           MSLKVKGGTEYAPELFDEQKD+VIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR
Sbjct: 181 MSLKVKGGTEYAPELFDEQKDRVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 240

Query: 342 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 385
           KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS
Sbjct: 241 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 270

BLAST of Carg12770 vs. NCBI nr
Match: XP_022976559.1 (vesicle-associated protein 4-1-like [Cucurbita maxima])

HSP 1 Score: 514.2 bits (1323), Expect = 9.5e-142
Identity = 266/283 (93.99%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKAS 161
           MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHH+RNGPKDRNSTASNNVKAS
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHSRNGPKDRNSTASNNVKAS 60

Query: 162 SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 221
           SSS TVS VAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA
Sbjct: 61  SSSKTVSFVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 120

Query: 222 PKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKI 281
           PKSCYMRPPGGILAPEEIFIAT             VFRFVEHPENNEKPKDHKSKIKFKI
Sbjct: 121 PKSCYMRPPGGILAPEEIFIAT-------------VFRFVEHPENNEKPKDHKSKIKFKI 180

Query: 282 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 341
           MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR
Sbjct: 181 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 240

Query: 342 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 385
           KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVG VDSVLS
Sbjct: 241 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGTVDSVLS 270

BLAST of Carg12770 vs. NCBI nr
Match: XP_023535121.1 (vesicle-associated protein 4-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.1 bits (1315), Expect = 8.0e-141
Identity = 266/283 (93.99%), Postives = 266/283 (93.99%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKAS 161
           M MTVADRHKP ADVKFFRLCSIWQSGNTSSSSLSTQNIHH RNGPKDRNSTASNNVKAS
Sbjct: 1   MIMTVADRHKPPADVKFFRLCSIWQSGNTSSSSLSTQNIHHARNGPKDRNSTASNNVKAS 60

Query: 162 SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 221
           SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA
Sbjct: 61  SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 120

Query: 222 PKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKI 281
           PKSCYMRPPGGILAPEEIFIAT             VFRFVEHPENN KPKDHKSKIKFKI
Sbjct: 121 PKSCYMRPPGGILAPEEIFIAT-------------VFRFVEHPENNGKPKDHKSKIKFKI 180

Query: 282 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 341
           MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR
Sbjct: 181 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 240

Query: 342 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 385
           KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS
Sbjct: 241 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 270

BLAST of Carg12770 vs. NCBI nr
Match: XP_038900245.1 (vesicle-associated protein 4-1 [Benincasa hispida])

HSP 1 Score: 466.5 bits (1199), Expect = 2.3e-127
Identity = 241/282 (85.46%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHTRNGPKDRNSTASNNVKA 161
           MTM VADRHKPHAD+K FRLC IWQSGNT SSSS STQN+HH+ +GP DRNST SNN+KA
Sbjct: 1   MTMAVADRHKPHADIKLFRLCPIWQSGNTNSSSSSSTQNLHHSSHGPSDRNSTVSNNMKA 60

Query: 162 SSSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTT 221
           S+SS TVSSVAKSLLPPRRRL LDPNNSLFFPYEPGKQVKSAIR+KNTSRSHVAFKFQTT
Sbjct: 61  SASSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRLKNTSRSHVAFKFQTT 120

Query: 222 APKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFK 281
           APKSCYMRPPGGILAP E FIAT             VFRFVEHPENNEKP D KSKIKFK
Sbjct: 121 APKSCYMRPPGGILAPGENFIAT-------------VFRFVEHPENNEKPMDQKSKIKFK 180

Query: 282 IMSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEA 341
           IMSLKVKGGTEYAPELF+EQKDQV VERILRV+FVDPERPSAAL++LKRQLAEAEAALEA
Sbjct: 181 IMSLKVKGGTEYAPELFEEQKDQVTVERILRVVFVDPERPSAALDKLKRQLAEAEAALEA 240

Query: 342 RKKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           RKKPP E+GPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV
Sbjct: 241 RKKPPVETGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 269

BLAST of Carg12770 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.8e-90
Identity = 186/283 (65.72%), Postives = 218/283 (77.03%), Query Frame = 0

Query: 104 MTVADRHKPHADVK--FFRLCSIWQSGNTSSSSLSTQNIHHT-RNGPKDRNSTASNNVKA 163
           M + DR  P  + K   FRLC  WQ  +T+SSS STQN +   R+   +RN+  S     
Sbjct: 1   MPIGDRQNPSVEKKKNLFRLCPFWQRRSTTSSS-STQNPNQNYRSRHGNRNTDIS---AV 60

Query: 164 SSSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTT 223
           S   +T+SSVA+SLLP RRRL LDP++ L+FPYEPGKQV+SAI++KNTS+SH AFKFQTT
Sbjct: 61  SKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTT 120

Query: 224 APKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEK-PKDHKSKIKF 283
           APKSCYMRPPGG+LAP E   AT             VF+FVEHPENNEK P + KSK+KF
Sbjct: 121 APKSCYMRPPGGVLAPGESVFAT-------------VFKFVEHPENNEKQPLNQKSKVKF 180

Query: 284 KIMSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALE 343
           KIMSLKVK G EY PELFDEQKDQV VE++LRVIF+D +RPSAALE+LKRQL EAEAA+E
Sbjct: 181 KIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAVE 240

Query: 344 ARKKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           ARKKPP E+GP+VV GEGLVIDEWKERREKYLARQQV +VDS+
Sbjct: 241 ARKKPPPETGPRVV-GEGLVIDEWKERREKYLARQQVESVDSL 265

BLAST of Carg12770 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 7.0e-79
Identity = 166/274 (60.58%), Postives = 199/274 (72.63%), Query Frame = 0

Query: 123 SIWQSGNTSSSSLSTQNIHHTR--------------NGPKDRNSTASNNVKASSSSMTVS 182
           S + SG +SSS+ S  + HH                NGP   +   S   +  + S +VS
Sbjct: 39  SPFPSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGP---HGDGSGQNQHPTPSPSVS 98

Query: 183 SVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMR 242
           SVAKS LP +RRL LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MR
Sbjct: 99  SVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMR 158

Query: 243 PPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKG 302
           PPG ILAP E  IAT             VF+FVE PENNEKP D +S++KFKIMSLKVKG
Sbjct: 159 PPGAILAPGETIIAT-------------VFKFVEPPENNEKPMDQRSRVKFKIMSLKVKG 218

Query: 303 GTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAES 362
             +Y PELFDEQKD V  E+ILRVIF+DPER + ALE+LKRQLAEA+AA+EARKKPP E+
Sbjct: 219 PMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEET 278

Query: 363 GPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           GP+++ GEGLVIDEWKERRE+YLA+QQ    DSV
Sbjct: 279 GPKMI-GEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of Carg12770 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 6.5e-69
Identity = 147/260 (56.54%), Postives = 191/260 (73.46%), Query Frame = 0

Query: 127 SGNTSSSSLSTQNIHHTR----NGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRRLH 186
           S + +SS L+   IH +R    +GP        N+ +++++  ++SSVA+SLLP +RRL 
Sbjct: 42  SSSVTSSHLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLK 101

Query: 187 LDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIA 246
           LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTT PKSC+MRP G ILAP E  IA
Sbjct: 102 LDPSAKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIA 161

Query: 247 TGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQKD 306
           T             VF+FVE PENNEKP + KS +KFKIMSLK+K  T+Y PELF+EQKD
Sbjct: 162 T-------------VFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKD 221

Query: 307 QVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKK-PPAESGPQVVGGEGLVID 366
            V  E+++RV+F+DPE P++ +E+LK QLAEA+AA EARKK      GP+ + GEGLVID
Sbjct: 222 HVSEEQVMRVVFLDPENPNSMMEKLKSQLAEADAADEARKKASEGIVGPKPI-GEGLVID 281

Query: 367 EWKERREKYLARQQVGAVDS 382
           EWK+RRE+YLA+QQ G VD+
Sbjct: 282 EWKQRRERYLAQQQ-GGVDA 286

BLAST of Carg12770 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.6e-06
Identity = 38/138 (27.54%), Postives = 63/138 (45.65%), Query Frame = 0

Query: 191 FPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIATGNVSTVM 250
           FP E  KQ   ++ + N + ++VAFK QTT PK   ++P  G++ P     ++  V  VM
Sbjct: 16  FPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPR----SSCEVLVVM 75

Query: 251 IFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGT---EYAPELFDEQKDQVIVE 310
                            E P D + K K       V+ GT   E   E+F ++    + E
Sbjct: 76  -------------QALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEE 135

Query: 311 RILRVIFVDPERPSAALE 326
            I ++I+V P +P + ++
Sbjct: 136 TIFKIIYVAPPQPQSPVQ 136

BLAST of Carg12770 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-05
Identity = 40/133 (30.08%), Postives = 60/133 (45.11%), Query Frame = 0

Query: 188 SLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIATGNVS 247
           +L F  +  KQ    +++ NT+  +VAFK +TT+PK   +RP  G++AP+         S
Sbjct: 12  TLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPK---------S 71

Query: 248 TVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGT---EYAPELFDEQKDQV 307
           T     +   F+        E P D   K KF I S  V   T   +    +F + + + 
Sbjct: 72  TCEFTVIMQAFK--------EPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKH 127

Query: 308 IVERILRVIFVDP 318
           I E  LRV  V P
Sbjct: 132 IEENKLRVTLVPP 127

BLAST of Carg12770 vs. ExPASy TrEMBL
Match: A0A6J1F9Z2 (vesicle-associated protein 4-1-like OS=Cucurbita moschata OX=3662 GN=LOC111443622 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.9e-143
Identity = 268/283 (94.70%), Postives = 270/283 (95.41%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKAS 161
           MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLST+NIHHTRNGPKDRNSTASNNVKAS
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTRNIHHTRNGPKDRNSTASNNVKAS 60

Query: 162 SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 221
           SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA
Sbjct: 61  SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 120

Query: 222 PKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKI 281
           PKSCYMRPPGGILAPEEIFIAT             VFRFVEHPENNEKPKDHKSKIKFKI
Sbjct: 121 PKSCYMRPPGGILAPEEIFIAT-------------VFRFVEHPENNEKPKDHKSKIKFKI 180

Query: 282 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 341
           MSLKVKGGTEYAPELFDEQKD+VIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR
Sbjct: 181 MSLKVKGGTEYAPELFDEQKDRVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 240

Query: 342 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 385
           KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS
Sbjct: 241 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 270

BLAST of Carg12770 vs. ExPASy TrEMBL
Match: A0A6J1INV3 (vesicle-associated protein 4-1-like OS=Cucurbita maxima OX=3661 GN=LOC111476917 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.6e-142
Identity = 266/283 (93.99%), Postives = 267/283 (94.35%), Query Frame = 0

Query: 102 MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHTRNGPKDRNSTASNNVKAS 161
           MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHH+RNGPKDRNSTASNNVKAS
Sbjct: 1   MTMTVADRHKPHADVKFFRLCSIWQSGNTSSSSLSTQNIHHSRNGPKDRNSTASNNVKAS 60

Query: 162 SSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 221
           SSS TVS VAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA
Sbjct: 61  SSSKTVSFVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTA 120

Query: 222 PKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKI 281
           PKSCYMRPPGGILAPEEIFIAT             VFRFVEHPENNEKPKDHKSKIKFKI
Sbjct: 121 PKSCYMRPPGGILAPEEIFIAT-------------VFRFVEHPENNEKPKDHKSKIKFKI 180

Query: 282 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 341
           MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR
Sbjct: 181 MSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEAR 240

Query: 342 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSVLS 385
           KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVG VDSVLS
Sbjct: 241 KKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGTVDSVLS 270

BLAST of Carg12770 vs. ExPASy TrEMBL
Match: A0A5A7T1W8 (Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20G00390 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 4.5e-121
Identity = 234/280 (83.57%), Postives = 244/280 (87.14%), Query Frame = 0

Query: 104 MTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHTRNGPKDRNSTASNNVKASS 163
           M VADR K HADVK FRLC IWQSGNT SSSS ST N+HHT +GP DR     NN+KASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 164 SSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 223
           SS TVSSVAKSLLPPRRRL LDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 224 KSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIM 283
           KSCYMRPPGGILAP E FIAT             VFRFVEHPENNE+P D KSKIKFKIM
Sbjct: 121 KSCYMRPPGGILAPGENFIAT-------------VFRFVEHPENNERPMDQKSKIKFKIM 180

Query: 284 SLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARK 343
           SLKVKGGTEYAPELF+EQKDQV VERILRV+FVDPERPSAALE+LKRQLAEAEAALEARK
Sbjct: 181 SLKVKGGTEYAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARK 240

Query: 344 KPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           KPP E+GPQV+GGEGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 KPPVETGPQVIGGEGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Carg12770 vs. ExPASy TrEMBL
Match: A0A1S3BZ62 (vesicle-associated protein 4-1 OS=Cucumis melo OX=3656 GN=LOC103494990 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 4.5e-121
Identity = 234/280 (83.57%), Postives = 244/280 (87.14%), Query Frame = 0

Query: 104 MTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHTRNGPKDRNSTASNNVKASS 163
           M VADR K HADVK FRLC IWQSGNT SSSS ST N+HHT +GP DR     NN+KASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 164 SSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 223
           SS TVSSVAKSLLPPRRRL LDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRRLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 224 KSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIM 283
           KSCYMRPPGGILAP E FIAT             VFRFVEHPENNE+P D KSKIKFKIM
Sbjct: 121 KSCYMRPPGGILAPGENFIAT-------------VFRFVEHPENNERPMDQKSKIKFKIM 180

Query: 284 SLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARK 343
           SLKVKGGTEYAPELF+EQKDQV VERILRV+FVDPERPSAALE+LKRQLAEAEAALEARK
Sbjct: 181 SLKVKGGTEYAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARK 240

Query: 344 KPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           KPP E+GPQV+GGEGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 KPPVETGPQVIGGEGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Carg12770 vs. ExPASy TrEMBL
Match: A0A5D3BAA2 (Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001120 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 1.0e-120
Identity = 233/280 (83.21%), Postives = 244/280 (87.14%), Query Frame = 0

Query: 104 MTVADRHKPHADVKFFRLCSIWQSGNT-SSSSLSTQNIHHTRNGPKDRNSTASNNVKASS 163
           M VADR K HADVK FRLC IWQSGNT SSSS ST N+HHT +GP DR     NN+KASS
Sbjct: 1   MAVADRPKSHADVKLFRLCPIWQSGNTNSSSSSSTHNLHHTTHGPSDR-----NNMKASS 60

Query: 164 SSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 223
           SS TVSSVAKSLLPPRR+L LDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP
Sbjct: 61  SSKTVSSVAKSLLPPRRKLRLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAP 120

Query: 224 KSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIM 283
           KSCYMRPPGGILAP E FIAT             VFRFVEHPENNE+P D KSKIKFKIM
Sbjct: 121 KSCYMRPPGGILAPGENFIAT-------------VFRFVEHPENNERPMDQKSKIKFKIM 180

Query: 284 SLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARK 343
           SLKVKGGTEYAPELF+EQKDQV VERILRV+FVDPERPSAALE+LKRQLAEAEAALEARK
Sbjct: 181 SLKVKGGTEYAPELFEEQKDQVTVERILRVVFVDPERPSAALEKLKRQLAEAEAALEARK 240

Query: 344 KPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           KPP E+GPQV+GGEGLVIDEWKERREKYLARQQVGA+DSV
Sbjct: 241 KPPVETGPQVIGGEGLVIDEWKERREKYLARQQVGALDSV 262

BLAST of Carg12770 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-91
Identity = 186/283 (65.72%), Postives = 218/283 (77.03%), Query Frame = 0

Query: 104 MTVADRHKPHADVK--FFRLCSIWQSGNTSSSSLSTQNIHHT-RNGPKDRNSTASNNVKA 163
           M + DR  P  + K   FRLC  WQ  +T+SSS STQN +   R+   +RN+  S     
Sbjct: 1   MPIGDRQNPSVEKKKNLFRLCPFWQRRSTTSSS-STQNPNQNYRSRHGNRNTDIS---AV 60

Query: 164 SSSSMTVSSVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTT 223
           S   +T+SSVA+SLLP RRRL LDP++ L+FPYEPGKQV+SAI++KNTS+SH AFKFQTT
Sbjct: 61  SKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTT 120

Query: 224 APKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEK-PKDHKSKIKF 283
           APKSCYMRPPGG+LAP E   AT             VF+FVEHPENNEK P + KSK+KF
Sbjct: 121 APKSCYMRPPGGVLAPGESVFAT-------------VFKFVEHPENNEKQPLNQKSKVKF 180

Query: 284 KIMSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALE 343
           KIMSLKVK G EY PELFDEQKDQV VE++LRVIF+D +RPSAALE+LKRQL EAEAA+E
Sbjct: 181 KIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAVE 240

Query: 344 ARKKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           ARKKPP E+GP+VV GEGLVIDEWKERREKYLARQQV +VDS+
Sbjct: 241 ARKKPPPETGPRVV-GEGLVIDEWKERREKYLARQQVESVDSL 265

BLAST of Carg12770 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 5.0e-80
Identity = 166/274 (60.58%), Postives = 199/274 (72.63%), Query Frame = 0

Query: 123 SIWQSGNTSSSSLSTQNIHHTR--------------NGPKDRNSTASNNVKASSSSMTVS 182
           S + SG +SSS+ S  + HH                NGP   +   S   +  + S +VS
Sbjct: 39  SPFPSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGP---HGDGSGQNQHPTPSPSVS 98

Query: 183 SVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMR 242
           SVAKS LP +RRL LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MR
Sbjct: 99  SVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMR 158

Query: 243 PPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKG 302
           PPG ILAP E  IAT             VF+FVE PENNEKP D +S++KFKIMSLKVKG
Sbjct: 159 PPGAILAPGETIIAT-------------VFKFVEPPENNEKPMDQRSRVKFKIMSLKVKG 218

Query: 303 GTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKKPPAES 362
             +Y PELFDEQKD V  E+ILRVIF+DPER + ALE+LKRQLAEA+AA+EARKKPP E+
Sbjct: 219 PMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEET 278

Query: 363 GPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           GP+++ GEGLVIDEWKERRE+YLA+QQ    DSV
Sbjct: 279 GPKMI-GEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of Carg12770 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.7e-76
Identity = 165/293 (56.31%), Postives = 199/293 (67.92%), Query Frame = 0

Query: 123 SIWQSGNTSSSSLSTQNIHHTR--------------NGPKDRNSTASNNVKASSSSMTVS 182
           S + SG +SSS+ S  + HH                NGP   +   S   +  + S +VS
Sbjct: 39  SPFPSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGP---HGDGSGQNQHPTPSPSVS 98

Query: 183 SVAKSLLPPRRRLHLDPNNSLFFPY-------------------EPGKQVKSAIRMKNTS 242
           SVAKS LP +RRL LDP+  L+FP+                   EPGKQV+SAI++KNTS
Sbjct: 99  SVAKSFLPTKRRLKLDPSEKLYFPWFVSEDLRSIDTSLKFLSFNEPGKQVRSAIKIKNTS 158

Query: 243 RSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEK 302
           +SHVAFKFQTTAPKSC+MRPPG ILAP E  IAT             VF+FVE PENNEK
Sbjct: 159 KSHVAFKFQTTAPKSCFMRPPGAILAPGETIIAT-------------VFKFVEPPENNEK 218

Query: 303 PKDHKSKIKFKIMSLKVKGGTEYAPELFDEQKDQVIVERILRVIFVDPERPSAALERLKR 362
           P D +S++KFKIMSLKVKG  +Y PELFDEQKD V  E+ILRVIF+DPER + ALE+LKR
Sbjct: 219 PMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKR 278

Query: 363 QLAEAEAALEARKKPPAESGPQVVGGEGLVIDEWKERREKYLARQQVGAVDSV 383
           QLAEA+AA+EARKKPP E+GP+++ GEGLVIDEWKERRE+YLA+QQ    DSV
Sbjct: 279 QLAEADAAVEARKKPPEETGPKMI-GEGLVIDEWKERRERYLAQQQGEGADSV 314

BLAST of Carg12770 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.6e-70
Identity = 147/260 (56.54%), Postives = 191/260 (73.46%), Query Frame = 0

Query: 127 SGNTSSSSLSTQNIHHTR----NGPKDRNSTASNNVKASSSSMTVSSVAKSLLPPRRRLH 186
           S + +SS L+   IH +R    +GP        N+ +++++  ++SSVA+SLLP +RRL 
Sbjct: 42  SSSVTSSHLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLK 101

Query: 187 LDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMRPPGGILAPEEIFIA 246
           LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTT PKSC+MRP G ILAP E  IA
Sbjct: 102 LDPSAKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIA 161

Query: 247 TGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKGGTEYAPELFDEQKD 306
           T             VF+FVE PENNEKP + KS +KFKIMSLK+K  T+Y PELF+EQKD
Sbjct: 162 T-------------VFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKD 221

Query: 307 QVIVERILRVIFVDPERPSAALERLKRQLAEAEAALEARKK-PPAESGPQVVGGEGLVID 366
            V  E+++RV+F+DPE P++ +E+LK QLAEA+AA EARKK      GP+ + GEGLVID
Sbjct: 222 HVSEEQVMRVVFLDPENPNSMMEKLKSQLAEADAADEARKKASEGIVGPKPI-GEGLVID 281

Query: 367 EWKERREKYLARQQVGAVDS 382
           EWK+RRE+YLA+QQ G VD+
Sbjct: 282 EWKQRRERYLAQQQ-GGVDA 286

BLAST of Carg12770 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 8.8e-45
Identity = 103/188 (54.79%), Postives = 124/188 (65.96%), Query Frame = 0

Query: 123 SIWQSGNTSSSSLSTQNIHHTR--------------NGPKDRNSTASNNVKASSSSMTVS 182
           S + SG +SSS+ S  + HH                NGP   +   S   +  + S +VS
Sbjct: 39  SPFPSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGP---HGDGSGQNQHPTPSPSVS 98

Query: 183 SVAKSLLPPRRRLHLDPNNSLFFPYEPGKQVKSAIRMKNTSRSHVAFKFQTTAPKSCYMR 242
           SVAKS LP +RRL LDP+  L+FPYEPGKQV+SAI++KNTS+SHVAFKFQTTAPKSC+MR
Sbjct: 99  SVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMR 158

Query: 243 PPGGILAPEEIFIATGNVSTVMIFSLFGVFRFVEHPENNEKPKDHKSKIKFKIMSLKVKG 297
           PPG ILAP E  IAT             VF+FVE PENNEKP D +S++KFKIMSLKVKG
Sbjct: 159 PPGAILAPGETIIAT-------------VFKFVEPPENNEKPMDQRSRVKFKIMSLKVKG 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024870.12.0e-216100.00Vesicle-associated protein 4-1, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022937296.13.9e-14394.70vesicle-associated protein 4-1-like [Cucurbita moschata][more]
XP_022976559.19.5e-14293.99vesicle-associated protein 4-1-like [Cucurbita maxima][more]
XP_023535121.18.0e-14193.99vesicle-associated protein 4-1-like [Cucurbita pepo subsp. pepo][more]
XP_038900245.12.3e-12785.46vesicle-associated protein 4-1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q1ECE01.8e-9065.72Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q8VYN27.0e-7960.58Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q8LPQ76.5e-6956.54Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q9SYC94.6e-0627.54Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
B9DHD71.3e-0530.08Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1F9Z21.9e-14394.70vesicle-associated protein 4-1-like OS=Cucurbita moschata OX=3662 GN=LOC11144362... [more]
A0A6J1INV34.6e-14293.99vesicle-associated protein 4-1-like OS=Cucurbita maxima OX=3661 GN=LOC111476917 ... [more]
A0A5A7T1W84.5e-12183.57Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3BZ624.5e-12183.57vesicle-associated protein 4-1 OS=Cucumis melo OX=3656 GN=LOC103494990 PE=4 SV=1[more]
A0A5D3BAA21.0e-12083.21Vesicle-associated protein 4-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT5G54110.11.3e-9165.72membrane-associated mannitol-induced [more]
AT4G21450.15.0e-8060.58PapD-like superfamily protein [more]
AT4G21450.35.7e-7656.31PapD-like superfamily protein [more]
AT4G05060.14.6e-7056.54PapD-like superfamily protein [more]
AT4G21450.28.8e-4554.79PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 321..344
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..142
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..171
NoneNo IPR availablePANTHERPTHR10809:SF110VESICLE-ASSOCIATED PROTEIN 4-1coord: 147..381
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 181..239
e-value: 2.9E-19
score: 68.9
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 180..315
score: 19.498949
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 177..319
e-value: 1.4E-25
score: 91.7
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 147..381
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 168..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12770-RACarg12770-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane