Carg12700 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12700
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRNA polymerase II transcriptional coactivator KIWI-like
LocationCarg_Chr07: 7525064 .. 7527429 (-)
RNA-Seq ExpressionCarg12700
SyntenyCarg12700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGAGGAAGAGATGTCTGCAAGAGGAAAGCGAAAGGACGATGAAGACCACGCATCCGAAGGAGATGCTCCTCCGAAGAAGACATTTAAGAAAAATTCCGACGAAACCGACGAAATCGTCGTCTGCGAGGTTCTTCATTTCTCTTCCTCCTTCTGTTCTTAGTGTCTTTTCCTCGCTTTCAACACTTTTAAATGTTTTTGAAATGTAGATTTCGAAGAACCGTAGAGTTATGGTGAGGAACTGGCAAGGAAAGATTGTGGTGGATATTCGGGAGTTTTATGTCAAAGATGGAAAGGAAATGCCTGGAAAAAAAGGTTCATCTCATCATCGTCTTCTTTTCTCTCTCTCTCTTTCTTTCGCAGTTGATGAATTCGACTTTCGTTTTGGATTTCTAGTTTGCGGCTTTTCTAGGGTTCATGTTCCACTTTGTGATTTTTATCGTCATCCATTTCAGTAGCATTTGCCTGGATTTTATTTATTATTTTTTCTTACGTTTTTTTTTTAAATTTATTTTTCTTTCTACAATTTGAGTTAGATGTTAGGGAAATGATCGAAAACTGCATCTATGACTTCACTATGGGGTGTTTGGTTCAAAGTGAAGAGGAATTTTGTTTTGGATGTTTGTATGTAAGCTTATGAAGTTTGGTGTATCTATATTTCATTTTATAGAAAATTTCTTTCTTTGGCTTCTGTGATGGCAAAATTTAGCGAGGTTTCAAAATTATTTTGTTTAAAATTTTGTTTTACTAATACTTTTCTTATCTAGTTTATGTAAATAGTTTGTTGGGAACATATAGGGAAGAATGAATATAAAGAGGATGAAAGAATAGATATTAAAGAGCTGGGTGAAGGATTTGAAGGAAAAAAGTACGGAGGGTGAAGAAGAGACTTGAGCGTGTTCAAATTTGCTGGAAACATATCATTGTTGTAACAAGACGTCAATTTCACGATGATTGATACAATATTAAGGAACCTTAAGGAAGTTTATGCATGAAGATTGCATATTTAGTCCTCTCATAGTGGCCAAAGCTTTCCTGAGAGGAGTAGTCAAGGAGGAGTTAGAGGAACTTTATAAGATATAGATGAAGGGTGGAGGATAGTGGGGGCTTTCTCACCCAAGTATGAGAGGTGGGACGCTGGAAAACATGGTTAAGTCAAAGTAATGCCATGCTATGGGGGATGCGTTAAGCTTCATGAATACCTTTGATGTTATGACAAGAAGAGATTTTATCAATATGTTAGATTGCTTGGAGGCTGTTATAAAACTATTGTGGCTTCTCCCCTTTGAAACTGAGATTGTGTATCCTAAAGAAGGAAGCTTTGTCATTCAAATAGTTGTAAATCAATCATCAAGATTGTTAGTTGGTAGAGTGAGGAGTGCCCATGGAGGTTTTTTAACATAAGCAACTACTAATTTCTAGGTGGTTTGTTTGGGTAGAATAACTGGTGTTGAAACTTCAATTGAAGCAGTAGTAGAAGGTTGGCCAAACAAGTTTTTTTCACAATTCATGCTGATAACATGGGGTTTTAAATGAGAGATAATTTTGTTGTTGAAGATTAGTTAAACAAGTTGCTGTTGGAGGAGAAGGTGAAACGTCTCGGTTTTGAAGAAGAAGAGAATGAGTTGTTTAAAAATAAATACTTTGCTAATTTGATTGAAGATGTTGGAAGAGGAAAAGTCATATGTTTATGAGCTTGTATGGAAAATGAAGTTTAAGTGTGTTTCAGGTGGTGGTTTAAAGGGTTCAAGAAAAAAAAAAATTCTAGATTGCTATTGCGTTGGGTTTTCCTTTGTTCTCCTAATGGAATGTTTGGAAGCTCTTGGTTTCATTTTTTTTTTTTATAAATGCTAACTTACCATGATCTATATTATTGACATGACGTTGCATATAATACTGCAAATGTAGAAGTTGAGTATGTCTTTTGATTTTCTTTTTGTTTACAGGCATCTCGCTATCCTTGGATCAGGTGTTGTGCACTTCTACTTCCTTAAAATTTTCAATTTTCAATTCTGTTATTAAAAACAATTAACTTTTCAATTTTCTTTAATCGTCGTCTTTCTGCAGTGGAACGTTCTTCGAAATCACGTAGAAGAAATCGACAAAGCTGTTAATGAAAATTCGTGAGGAAATTAGATCGACATCTTATGTTGATATTAGTAGTAACTGAATCACTCTGTAGGGCTATTCGACTTTATGAGGACGGAAATATGACCTTTTGTACAGCTAATGGGCTTGTTTTTGTGGGCATGAAGACCAAAGAGCAGGTATTAGCATTTATATCTACATTAACTGCATCACTTAGATTGACATTTTGATATTGTAGACCAGAACATGATATTATAATATATTATGCCATAACTGAC

mRNA sequence

AAAGGAGGAAGAGATGTCTGCAAGAGGAAAGCGAAAGGACGATGAAGACCACGCATCCGAAGGAGATGCTCCTCCGAAGAAGACATTTAAGAAAAATTCCGACGAAACCGACGAAATCGTCGTCTGCGAGATTTCGAAGAACCGTAGAGTTATGGTGAGGAACTGGCAAGGAAAGATTGTGGTGGATATTCGGGAGTTTTATGTCAAAGATGGAAAGGAAATGCCTGGAAAAAAAGGCATCTCGCTATCCTTGGATCAGTGGAACGTTCTTCGAAATCACGTAGAAGAAATCGACAAAGCTGTTAATGAAAATTCGTGAGGAAATTAGATCGACATCTTATGTTGATATTAGTAGTAACTGAATCACTCTGTAGGGCTATTCGACTTTATGAGGACGGAAATATGACCTTTTGTACAGCTAATGGGCTTGTTTTTGTGGGCATGAAGACCAAAGAGCAGGTATTAGCATTTATATCTACATTAACTGCATCACTTAGATTGACATTTTGATATTGTAGACCAGAACATGATATTATAATATATTATGCCATAACTGAC

Coding sequence (CDS)

ATGTCTGCAAGAGGAAAGCGAAAGGACGATGAAGACCACGCATCCGAAGGAGATGCTCCTCCGAAGAAGACATTTAAGAAAAATTCCGACGAAACCGACGAAATCGTCGTCTGCGAGATTTCGAAGAACCGTAGAGTTATGGTGAGGAACTGGCAAGGAAAGATTGTGGTGGATATTCGGGAGTTTTATGTCAAAGATGGAAAGGAAATGCCTGGAAAAAAAGGCATCTCGCTATCCTTGGATCAGTGGAACGTTCTTCGAAATCACGTAGAAGAAATCGACAAAGCTGTTAATGAAAATTCGTGA

Protein sequence

MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS
Homology
[Close]
BLAST of Carg12700 vs. NCBI nr
Match: KAG7027581.1 (RNA polymerase II transcriptional coactivator KIWI [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 204.5 bits (519), Expect = 4.2e-49
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. NCBI nr
Match: XP_023518433.1 (RNA polymerase II transcriptional coactivator KIWI-like [Cucurbita pepo subsp. pepo] >KAG6595609.1 RNA polymerase II transcriptional coactivator KIWI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 202.6 bits (514), Expect = 1.6e-48
Identity = 100/101 (99.01%), Postives = 101/101 (100.00%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKTFKK+SDETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTFKKDSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. NCBI nr
Match: XP_022966500.1 (RNA polymerase II transcriptional coactivator KIWI-like isoform X1 [Cucurbita maxima] >XP_022966501.1 RNA polymerase II transcriptional coactivator KIWI-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 199.5 bits (506), Expect = 1.4e-47
Identity = 98/101 (97.03%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKTFKK+S+ETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTFKKDSEETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKG SLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGFSLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. NCBI nr
Match: XP_022925285.1 (RNA polymerase II transcriptional coactivator KIWI-like [Cucurbita moschata])

HSP 1 Score: 198.7 bits (504), Expect = 2.3e-47
Identity = 98/101 (97.03%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKT KK+S+ETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTLKKDSEETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. NCBI nr
Match: KAG6603408.1 (RNA polymerase II transcriptional coactivator KIWI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 197.6 bits (501), Expect = 5.2e-47
Identity = 96/101 (95.05%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDH SEGDAPPKKTF+K+SDETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHVSEGDAPPKKTFRKDSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGK+MPGKKGISLSLDQWNVLRNHV+EIDKAVNENS
Sbjct: 61  EFYVKDGKQMPGKKGISLSLDQWNVLRNHVDEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match: O65154 (RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=3702 GN=KIWI PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 4.1e-31
Identity = 70/107 (65.42%), Postives = 85/107 (79.44%), Query Frame = 0 

Query: 1   MSARGKRKDDE----DHASEGDAPPKKTFK--KNSDETDEIVVCEISKNRRVMVRNWQGK 60
           MS+RGKRKD++    D  SE  AP KK  K   +SD++D+IVVC ISKNRRV VRNW GK
Sbjct: 1   MSSRGKRKDEDVRASDDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGK 60

Query: 61  IVVDIREFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           I +DIREFYVKDGK +PGKKGISLS+DQWN LRNH E+I+KA+++ S
Sbjct: 61  IWIDIREFYVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKALSDLS 107

[Close]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match: O65155 (RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=3702 GN=KELP PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 7.3e-12
Identity = 37/92 (40.22%), Postives = 60/92 (65.22%), Query Frame = 0 

Query: 8   KDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYVKDG 67
           K++ED    GD    K  K+  D+ D +++C +S  RRV ++ ++GK +V IRE+Y KDG
Sbjct: 74  KEEED----GDKDCGKGNKEFDDDGD-LIICRLSDKRRVTIQEFKGKSLVSIREYYKKDG 133

Query: 68  KEMPGKKGISLSLDQWNVLRNHVEEIDKAVNE 100
           KE+P  KGISL+ +QW+  + ++  I+ AV +
Sbjct: 134 KELPTSKGISLTDEQWSTFKKNMPAIENAVKK 160

[Close]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match: P11031 (Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=10090 GN=Sub1 PE=1 SV=3)

HSP 1 Score: 64.7 bits (156), Expect = 6.9e-10
Identity = 30/74 (40.54%), Postives = 53/74 (71.62%), Query Frame = 0 

Query: 27  KNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYV-KDGKEMPGKKGISLSLDQWNV 86
           K S  + +  + +I K R V VR+++GKI++DIRE+++  +G+  PG+KGISL+++QW+ 
Sbjct: 53  KQSSSSRDDNMFQIGKMRYVSVRDFKGKILIDIREYWMDSEGEMKPGRKGISLNMEQWSQ 112

Query: 87  LRNHVEEIDKAVNE 100
           L+  + +ID AV +
Sbjct: 113 LKEQISDIDDAVRK 126

[Close]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match: Q63396 (Activated RNA polymerase II transcriptional coactivator p15 OS=Rattus norvegicus OX=10116 GN=Sub1 PE=1 SV=3)

HSP 1 Score: 64.3 bits (155), Expect = 9.0e-10
Identity = 31/84 (36.90%), Postives = 56/84 (66.67%), Query Frame = 0 

Query: 17  GDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYV-KDGKEMPGKKG 76
           G++       K S  + +  + +I K R V VR+++GKI++DIRE+++  +G+  PG+KG
Sbjct: 43  GESSRALASSKQSSSSRDDNMFQIGKMRYVSVRDFKGKILIDIREYWMDSEGEMKPGRKG 102

Query: 77  ISLSLDQWNVLRNHVEEIDKAVNE 100
           ISL+++QW+ L+  + +ID AV +
Sbjct: 103 ISLNMEQWSQLKEQISDIDDAVRK 126

[Close]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match: Q5ZK63 (Activated RNA polymerase II transcriptional coactivator p15 OS=Gallus gallus OX=9031 GN=SUB1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-09
Identity = 36/94 (38.30%), Postives = 62/94 (65.96%), Query Frame = 0 

Query: 7   RKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYV-K 66
           +K     +S+G A  K++   N DE     + +I K R V VR+++GK+++DIRE+++ +
Sbjct: 38  KKQKTGESSKGAASSKQS--SNRDEN----MFQIGKMRYVSVRDFKGKVLIDIREYWMDQ 97

Query: 67  DGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNE 100
           +G+  PG+KGISL+ +QWN L+  + +ID AV +
Sbjct: 98  EGEMKPGRKGISLNPEQWNQLKEQISDIDDAVRK 125

[Close]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match: A0A6J1HRT2 (RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466134 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.6e-48
Identity = 98/101 (97.03%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKTFKK+S+ETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTFKKDSEETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKG SLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGFSLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match: A0A6J1EHI0 (RNA polymerase II transcriptional coactivator KIWI-like OS=Cucurbita moschata OX=3662 GN=LOC111432568 PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.1e-47
Identity = 98/101 (97.03%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDHASEGDAPPKKT KK+S+ETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHASEGDAPPKKTLKKDSEETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match: A0A6J1EYI2 (RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439635 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-46
Identity = 95/101 (94.06%), Postives = 99/101 (98.02%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDDEDH  EGDAPPKKTF+K+SDETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDEDHVYEGDAPPKKTFRKDSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGK+MPGKKGISLSLDQWNVLRNHV+EIDKAVNENS
Sbjct: 61  EFYVKDGKQMPGKKGISLSLDQWNVLRNHVDEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match: A0A6J1D5M3 (RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017843 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.6e-46
Identity = 95/101 (94.06%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKRKDD++HASEGDAPPKKT KK+SDETDEIVVCEISKNRRVMVRNWQGKIVVDIR
Sbjct: 1   MSARGKRKDDDEHASEGDAPPKKTSKKDSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGK+MPGKKGISLS+DQWNVLRNHVEEIDKAVNENS
Sbjct: 61  EFYVKDGKQMPGKKGISLSMDQWNVLRNHVEEIDKAVNENS 101

[Close]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match: A0A6J1IRG1 (RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478052 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.4e-45
Identity = 92/101 (91.09%), Postives = 100/101 (99.01%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIR 60
           MSARGKR+DDEDH SEGDAPPKKTF+K+SDETDEIVVCEISKNR+V+VRNWQGKIVVDIR
Sbjct: 1   MSARGKRRDDEDHVSEGDAPPKKTFRKDSDETDEIVVCEISKNRKVIVRNWQGKIVVDIR 60

Query: 61  EFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           EFYVKDGK+MPGKKGISLSLDQWNVLRNHV+EIDKAV+ENS
Sbjct: 61  EFYVKDGKQMPGKKGISLSLDQWNVLRNHVDEIDKAVDENS 101

[Close]
BLAST of Carg12700 vs. TAIR 10
Match: AT5G09250.1 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 135.2 bits (339), Expect = 2.9e-32
Identity = 70/107 (65.42%), Postives = 85/107 (79.44%), Query Frame = 0 

Query: 1   MSARGKRKDDE----DHASEGDAPPKKTFK--KNSDETDEIVVCEISKNRRVMVRNWQGK 60
           MS+RGKRKD++    D  SE  AP KK  K   +SD++D+IVVC ISKNRRV VRNW GK
Sbjct: 1   MSSRGKRKDEDVRASDDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGK 60

Query: 61  IVVDIREFYVKDGKEMPGKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           I +DIREFYVKDGK +PGKKGISLS+DQWN LRNH E+I+KA+++ S
Sbjct: 61  IWIDIREFYVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKALSDLS 107

[Close]
BLAST of Carg12700 vs. TAIR 10
Match: AT5G09240.1 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-22
Identity = 59/109 (54.13%), Postives = 76/109 (69.72%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHA------SEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGK 60
           MS  GKRKD++  A      SE  APPKK   K +DE ++I +C + KNRRV VRN  G+
Sbjct: 1   MSENGKRKDEDVRASDDRDESETHAPPKKV-AKPADEIEDIFICNLDKNRRVFVRNCNGR 60

Query: 61  IVVDIREFYVKDGKEMP--GKKGISLSLDQWNVLRNHVEEIDKAVNENS 102
           I + IR+F+VKDG  +P   K+GISLSL+QWN LRNH E+IDKA++E S
Sbjct: 61  IWIAIRQFFVKDGITLPCNSKQGISLSLEQWNDLRNHEEDIDKALSELS 108

[Close]
BLAST of Carg12700 vs. TAIR 10
Match: AT5G09250.2 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 98.2 bits (243), Expect = 4.0e-21
Identity = 53/81 (65.43%), Postives = 62/81 (76.54%), Query Frame = 0 

Query: 1  MSARGKRKDDE----DHASEGDAPPKKTFK--KNSDETDEIVVCEISKNRRVMVRNWQGK 60
          MS+RGKRKD++    D  SE  AP KK  K   +SD++D+IVVC ISKNRRV VRNW GK
Sbjct: 1  MSSRGKRKDEDVRASDDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGK 60

Query: 61 IVVDIREFYVKDGKEMPGKKG 76
          I +DIREFYVKDGK +PGKKG
Sbjct: 61 IWIDIREFYVKDGKTLPGKKG 81

[Close]
BLAST of Carg12700 vs. TAIR 10
Match: AT5G09240.2 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 88.2 bits (217), Expect = 4.1e-18
Identity = 59/137 (43.07%), Postives = 76/137 (55.47%), Query Frame = 0 

Query: 1   MSARGKRKDDEDHA------SEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGK 60
           MS  GKRKD++  A      SE  APPKK   K +DE ++I +C + KNRRV VRN  G+
Sbjct: 1   MSENGKRKDEDVRASDDRDESETHAPPKKV-AKPADEIEDIFICNLDKNRRVFVRNCNGR 60

Query: 61  IVVDIREFYVKDGKEMP--GKKGISLSLD----------------------------QWN 102
           I + IR+F+VKDG  +P   K+GISLSL+                            QWN
Sbjct: 61  IWIAIRQFFVKDGITLPCNSKQGISLSLEQVFTSLFHCLCLPHVHMSCLLHETFFLMQWN 120

[Close]
BLAST of Carg12700 vs. TAIR 10
Match: AT4G10920.1 (transcriptional coactivator p15 (PC4) family protein (KELP) )

HSP 1 Score: 71.2 bits (173), Expect = 5.2e-13
Identity = 37/92 (40.22%), Postives = 60/92 (65.22%), Query Frame = 0 

Query: 8   KDDEDHASEGDAPPKKTFKKNSDETDEIVVCEISKNRRVMVRNWQGKIVVDIREFYVKDG 67
           K++ED    GD    K  K+  D+ D +++C +S  RRV ++ ++GK +V IRE+Y KDG
Sbjct: 74  KEEED----GDKDCGKGNKEFDDDGD-LIICRLSDKRRVTIQEFKGKSLVSIREYYKKDG 133

Query: 68  KEMPGKKGISLSLDQWNVLRNHVEEIDKAVNE 100
           KE+P  KGISL+ +QW+  + ++  I+ AV +
Sbjct: 134 KELPTSKGISLTDEQWSTFKKNMPAIENAVKK 160

The following BLAST results are available for this feature:
BLAST of Carg12700 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7027581.14.2e-49100.00RNA polymerase II transcriptional coactivator KIWI [Cucurbita argyrosperma subsp... [more]
XP_023518433.11.6e-4899.01RNA polymerase II transcriptional coactivator KIWI-like [Cucurbita pepo subsp. p... [more]
XP_022966500.11.4e-4797.03RNA polymerase II transcriptional coactivator KIWI-like isoform X1 [Cucurbita ma... [more]
XP_022925285.12.3e-4797.03RNA polymerase II transcriptional coactivator KIWI-like [Cucurbita moschata][more]
KAG6603408.15.2e-4795.05RNA polymerase II transcriptional coactivator KIWI, partial [Cucurbita argyrospe... [more]
BLAST of Carg12700 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O651544.1e-3165.42RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=37... [more]
O651557.3e-1240.22RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=37... [more]
P110316.9e-1040.54Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=1... [more]
Q633969.0e-1036.90Activated RNA polymerase II transcriptional coactivator p15 OS=Rattus norvegicus... [more]
Q5ZK631.2e-0938.30Activated RNA polymerase II transcriptional coactivator p15 OS=Gallus gallus OX=... [more]
BLAST of Carg12700 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HRT26.6e-4897.03RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita ... [more]
A0A6J1EHI01.1e-4797.03RNA polymerase II transcriptional coactivator KIWI-like OS=Cucurbita moschata OX... [more]
A0A6J1EYI21.2e-4694.06RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita ... [more]
A0A6J1D5M31.6e-4694.06RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Momordica ... [more]
A0A6J1IRG11.4e-4591.09RNA polymerase II transcriptional coactivator KIWI-like isoform X1 OS=Cucurbita ... [more]
BLAST of Carg12700 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G09250.12.9e-3265.42ssDNA-binding transcriptional regulator [more]
AT5G09240.11.2e-2254.13ssDNA-binding transcriptional regulator [more]
AT5G09250.24.0e-2165.43ssDNA-binding transcriptional regulator [more]
AT5G09240.24.1e-1843.07ssDNA-binding transcriptional regulator [more]
AT4G10920.15.2e-1340.22transcriptional coactivator p15 (PC4) family protein (KELP) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003173Transcriptional coactivator p15 (PC4), C-terminalPFAMPF02229PC4coord: 38..88
e-value: 1.9E-24
score: 85.1
IPR009044ssDNA-binding transcriptional regulatorGENE3D2.30.31.10Transcriptional Coactivator Pc4; Chain Acoord: 26..101
e-value: 1.7E-27
score: 96.8
IPR009044ssDNA-binding transcriptional regulatorSUPERFAMILY54447ssDNA-binding transcriptional regulator domaincoord: 37..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR13215:SF9BNAC09G47220D PROTEINcoord: 1..99
IPR045125RNA polymerase II transcriptional coactivator Sub1/Tcp4-likePANTHERPTHR13215RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATORcoord: 1..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12700-RACarg12700-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription regulator complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003713 transcription coactivator activity