Carg12600 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12600
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioninteractor of constitutive active ROPs 2, chloroplastic
LocationCarg_Chr14: 808699 .. 813896 (-)
RNA-Seq ExpressionCarg12600
SyntenyCarg12600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCGCCGCGTTACGGTGGTTCCGCCGTACGCGCCTTCTCAGGTCCACCGTGTCTCTCCCTCTCTCTCCCACGGTTCCTCCGCCGGTACGGCCGCCCCACACCTACTCCCATGTGCGGCACTAACCCAGTTGTTTCACAACGCAATAAAACTTGTAATATTTTTATATTGATTATTTGCTTTATTTATTGGAATTTGAACTTTCTAGCTCTAAGAAAATAATATATATAAGAACTACCACCGAATTATTTCATTTTGAAAAAATTAATCATGATAATATTATATTATTTATTTAAAAATTATATTTATTATTTTTTAAATTTGGTTTCAAAATTATATTCAGTAGATATTTTTTAAAATTTTAGACCAAAATCTCGTTACAAGATTTTTACTTCCAAAGTGGTTTAGAAGCGATTATAAAGCATATTTTATTTATTTATTGCGAAAATTAAAAAGAAAAAAAAAAAGAAATGATTTTAGGAAATGGTTGCTTGGCTTAGAATCAAGTTGAATGTTGATGCTGATTCAATGATGATTCAGAGCACAAGCTCATAGGAATGTAACATTTGGGTCATTGTGGCCAACATCAATGCCTCCCACTTATGGTCACATAAAATAAAACAGATAAAATAATCTCCTCATTATTATTTAAATTAATTTATAATTTAAAACTCAATAAATATAATTGAAATTAGATACTAAAAAAAAAAAATCATTTTATAACTAAGCTTTCAAAAATGAAAACATAAAAGGAGAATGTTAATATTTTAAAAATTAATTTGAGTCCACATCTAATAATTATTAAATAAAGTTTTCAATCTTTTGTGAAATGTATCTATTTTATTTTATTCATTTATTATTTTATTTTATATATATTTTTTAAATTCACAAATTTATTAGATATATAAAACGTGTCATTAGGACCACCACCTTCTTGATAAACTTCGATGTTTATAAATTTGGTGATTCCTCTAACATCAAATTAAAAAATTCAATAATTTATTATACATTTTGAAAGTCGAGAAACTTATTAAATACATATTAAATTTTTTTAAAAAAATTCGACAATAAACTCAAAATTAATTTAAATTTAAAATTAGATTGATAATTTTAAACTTTGGATATTATATAATTATAGTATCTAAACTTTAAATTGAAATTTATAAAGCAATCAATTTAGATTATTTTCCATAAAAAACAATAATTGGAGAAAAAACGTGATTGGAGGGTGAAGAAGCGCGTGAGGCATGAGGAGCGTGGCCCAAAAAAATGGCCACTGAATAAAGAAATGTATCGTACTACTGATTTTACGTTGCTTTTCTTCTTCACTCGCTCCCTCCCACTGACATAACATAACAGACTTTTGAAGAGTGAGAAATGGAAAGAGAACTCGGGAACTGAGCACAAAACCAGTTGAAGAAGATGATGAAGAACTAGCACCTCCATTTCACCATTCCTTTGCAGCCTCAACTCAAAAAACTAGGGTTTCTGTAATTTCAAGCGAATTTGTTAAGGAAAATCGCCACCAGCATCAGCTGCATCAGCAGATTTCCATTTTCGGGTAACAACACTCTGTTTTACTGCCTCCTTCCGCTTCGCCAAATGCTGTTTGTTTGAGAATAATAGCCAATATATGCGCCATTTCTGTTCTACTTGCTGAAAGATCTGTTTTCTTTTGACGTGCGCTTTGATTTCGCGTAATGTTAAGCGTTTTGGAAGCAATCGTCGCGTTTTCCGGATGGTTTTGATTTTTACGATGCGAAACTGTTGAGGTTTTGCTTCTTTCTGGAAGTCTCTTCGTCTTCTATGGCGAGTTTGGTGTTGTGATTCTGCTTATTGTTTGTTTTTATGTTGAATACTTTATACTCTCAGATCTGTGAGTTCAGTTTATTTACCCAACTGGGCCGACTCTGAAAGCCCTTCTTCACCATTCTCCATTAATATTTGTTTTGCTTCTTCCTCGGATATGTTTGTTTCTTTAATCTACTTGTTAGTGCTTTTTATGATCCACCATTGAATACATTGCTTTATAGTTTGTTAACTTCATACTTTTTCCATTTCTAAGTGTATCTGGAATTAAAATACTCAAATGAAAGCTAATTTTTCTCTGAAAAACCCGGCGGAATACACCTTAAATACGAAATTTCATGTAATAATTCGTTTGAATGAATTTCAGGGGCCCTTGGGCCAGAGTAGTGCGTTTCAAACAGAGGTCACCGTAGGGCAAAAAGGAGGGGCAAAAGGGAAAAGGGAAATGTTAAAAGTCCGAAACATGTTTTTTGCCTAATGATAAATATAGGGTTCTCTTTCTTACAATTTTCCATTTACTTTCATATTTGAAAAAAGGCTAAATTGAAATGGATTTGGCTTTTGTTGGTTGTAGTGGGGTTAGTTTTTTGGAGTTTCTTTGAAGGGATTGCATTGTGTGTTGTCTTTTTTGTTAAGTTTGGTACTGATGTTGATCTCTGGTAGTGAGGCTGCTATCCTTTGTGAATAATTTTTGTACAAACATATATTGGCTTCCCTTTTCTGTTCTTTTTCTCACGCAAGATTCTCTCGGCTCTTTCAGGTACTGTGGGTCAGAAGACTCTATTAACTCAATTATTTTATCTGCTTGATTATTTTTCTAAGGGAAACACACAAGCATGCAGACACCCAAAGCAAAGTAAGGCTATCGTTTAGTTTTTGAATTGCATAGCTGTTTGCACCCTCTGGCCGCTTAATCGAACGGATTTCTTTTGTTCTTTATCTTTTGGCTTCCCACTTTTCATTCTTGATTTCTGATTGTTTTTAACTGTAATCTGAAATGTTTATGTTTTACTCAAAGAAGTAATACTGTCCATGATGCAATCATGCAAGGTTTTAGATGAGGTAAATTACACGATCTAGTTGTGAGTTGGGTCGGTTTGAGAGATTGATTTGCAGATAAAAATTCACGTTACTCGAGAGAAAAGTGTGGAATTTGACCTCTCCTTATACGTGGGGTCTAAAGATATGTATAGTTTAACATTTAGGATCATGATTGAAGTGATAATATATAATTTAAATTATTATCAGAGATTCAGTGAAGACTGATCAGAGATTGATATGTTCTTAATTACAAGTCAAGCTATGCTGAACACAGAATATGCATGGTAGCTTATCCTCCATTGATTTGTGTGTTTATCCGCTATCCTCTCTCAGGATAAAAGACACTTTTGGTTCATTGGCACCTAGTGGTATAGCTAAGTAGCAACGTTATATTATGGGCAGATATTAAAAGTTGTTTAAATTGGACTGCAGAGTTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCCAGGACGCCTAGAACCGCTCGTCAACTTAAAACTCCAAGCTCACATCCTGATTCCGTTTCCACTTCTCCACTTGCTGCTAGCAAGTCACCGAGAGAGAGGAGTCCTAGGGTGGTGACTGACCGCAAGTCACCACGATCTCTGGCCACTGAGGTACTTGTTTTACTTTTTATGACCAATGATAGATTATACCATATCACTTTGATTTGTTGATATTAGGATTTTTTTTCTTCTCATTTCAGAGTAAAGTGCCTAGCAAAGTGGCTGAATTGGAATCACAGCTTTCTCAACTCCAGGAGGAACTCAAGAAGACAAGTGACCAACTGAGCGCATCTGAATCAGACAAAAGACAGGCTCAACAGGAGGCAGAAGAAGCAAAGAAACAACTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGCTCGAGCTCTCCAATTCGGAGGAAGATCGTGTTCAAGAACTGCACAAAATTTCTCAAGATCGTGATCGAGCATGGCAGTCTGAGCTTGAGGCCATTCAAAAGCAACATTCAATTGATGCTGCTTCATTGGCCTCTGCCATGAATGAAGTTCAGAGGCTCAAGGTCCAGTTGGAGACGGCGTCTGAATCTGAGGCAACCCAGAGTAAGCTTGCTGAGTCTGCACAAACTGAGATAGATTGTTTAAGAACCCAGCTATCAGAGACTCTCTCCCTAGTTGAAAAACTGAAAGATGAGCTTAGTTACTGTAAAGAATCTGAAGCTCAAGCTCTTGAAGTTGCAAGGAAAAATCATAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTGCAATCGGATGGAATTAAAGCCATTGACGCTTACAACTCTCTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAAATCAGCAAGAAGATTCAGAAAGGTTTGTTAGATTCTGAAGACAATGGTTTAGATCCTGAAGTCAAAGCAAAAGCTGCTGAGCAAAACGATGGAAAGGATGATATTAGTAAGGTAAAAACTGAGCTAACCGCTTTGAGATTAGAAGCAGATAAGTCAAAATCTGCATTGGAGGCAGCTGAGGCAAGATTCGAGGAGGAATATGTTCGTACAACATTGCAAATTAGAATTGCTCACGAACTTGTGGAACAAATGAAAGTAGAGTCATGTCAGAAAGAGGCCGAATTGGATGCAGAACTTAAGGAGACCAGGGCGAACTTAGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAACTGTGTAGCATTGTGGAGGAGAACAAGGAGCTCAACTCAGAGATAAGCAAAATGATACCAGTCGACAGGGAGTCCGAACTAGCAATGGAACTACAAAGGTTAGAGGCTGATATGGTGGAGCTGAAGAACAGGCTCTTGGAAAAGGAAACACAGCTGCAGGGTACAACAGAGGAAAATGATGCACTCAAGAAGGAAATTGAGAAGATCAATGAGGAAGCGGTTAGTATAGCAGAAACAACCAAAGCTACAGAGAAGGAGGCGCTGATGAAACTCGAGCACGCAACAGAAGAGGCAGATAAAAGTAGCCGAAGAGCGGCACGAGTGGCCGAGCAATTGGATGCTGCACAGGCTGCAAGCTCTGAAATGGAAGCTGAGCTGAGGAGGTTGAAAGTGCAAGCAGAGCAGTGGAGGAAGGCAGCTGAGGCAGCTGCTGCAATACTCTCAACTGGGAACAATGGGAAGATTGTTGATAAACTAGTTTGTTTGGAGAACAATTACCCTATGGGGTCACCATACTCGGAAGATCTTGAGGCGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAGAGCAGAAGAAAACTGGTTAC

mRNA sequence

GGCCGCCGCGTTACGGTGGTTCCGCCGTACGCGCCTTCTCAGGTCCACCGTGTCTCTCCCTCTCTCTCCCACGGTTCCTCCGCCGCCTCAACTCAAAAAACTAGGGTTTCTGTAATTTCAAGCGAATTTGTTAAGGAAAATCGCCACCAGCATCAGCTGCATCAGCAGATTTCCATTTTCGGAGTTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCCAGGACGCCTAGAACCGCTCGTCAACTTAAAACTCCAAGCTCACATCCTGATTCCGTTTCCACTTCTCCACTTGCTGCTAGCAAGTCACCGAGAGAGAGGAGTCCTAGGGTGGTGACTGACCGCAAGTCACCACGATCTCTGGCCACTGAGAGTAAAGTGCCTAGCAAAGTGGCTGAATTGGAATCACAGCTTTCTCAACTCCAGGAGGAACTCAAGAAGACAAGTGACCAACTGAGCGCATCTGAATCAGACAAAAGACAGGCTCAACAGGAGGCAGAAGAAGCAAAGAAACAACTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGCTCGAGCTCTCCAATTCGGAGGAAGATCGTGTTCAAGAACTGCACAAAATTTCTCAAGATCGTGATCGAGCATGGCAGTCTGAGCTTGAGGCCATTCAAAAGCAACATTCAATTGATGCTGCTTCATTGGCCTCTGCCATGAATGAAGTTCAGAGGCTCAAGGTCCAGTTGGAGACGGCGTCTGAATCTGAGGCAACCCAGAGTAAGCTTGCTGAGTCTGCACAAACTGAGATAGATTGTTTAAGAACCCAGCTATCAGAGACTCTCTCCCTAGTTGAAAAACTGAAAGATGAGCTTAGTTACTGTAAAGAATCTGAAGCTCAAGCTCTTGAAGTTGCAAGGAAAAATCATAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTGCAATCGGATGGAATTAAAGCCATTGACGCTTACAACTCTCTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAAATCAGCAAGAAGATTCAGAAAGGTTTGTTAGATTCTGAAGACAATGGTTTAGATCCTGAAGTCAAAGCAAAAGCTGCTGAGCAAAACGATGGAAAGGATGATATTAGTAAGGTAAAAACTGAGCTAACCGCTTTGAGATTAGAAGCAGATAAGTCAAAATCTGCATTGGAGGCAGCTGAGGCAAGATTCGAGGAGGAATATGTTCGTACAACATTGCAAATTAGAATTGCTCACGAACTTGTGGAACAAATGAAAGTAGAGTCATGTCAGAAAGAGGCCGAATTGGATGCAGAACTTAAGGAGACCAGGGCGAACTTAGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAACTGTGTAGCATTGTGGAGGAGAACAAGGAGCTCAACTCAGAGATAAGCAAAATGATACCAGTCGACAGGGAGTCCGAACTAGCAATGGAACTACAAAGGTTAGAGGCTGATATGGTGGAGCTGAAGAACAGGCTCTTGGAAAAGGAAACACAGCTGCAGGGTACAACAGAGGAAAATGATGCACTCAAGAAGGAAATTGAGAAGATCAATGAGGAAGCGGTTAGTATAGCAGAAACAACCAAAGCTACAGAGAAGGAGGCGCTGATGAAACTCGAGCACGCAACAGAAGAGGCAGATAAAAGTAGCCGAAGAGCGGCACGAGTGGCCGAGCAATTGGATGCTGCACAGGCTGCAAGCTCTGAAATGGAAGCTGAGCTGAGGAGGTTGAAAGTGCAAGCAGAGCAGTGGAGGAAGGCAGCTGAGGCAGCTGCTGCAATACTCTCAACTGGGAACAATGGGAAGATTGTTGATAAACTAGTTTGTTTGGAGAACAATTACCCTATGGGGTCACCATACTCGGAAGATCTTGAGGCGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAGAGCAGAAGAAAACTGGTTAC

Coding sequence (CDS)

GGCCGCCGCGTTACGGTGGTTCCGCCGTACGCGCCTTCTCAGGTCCACCGTGTCTCTCCCTCTCTCTCCCACGGTTCCTCCGCCGCCTCAACTCAAAAAACTAGGGTTTCTGTAATTTCAAGCGAATTTGTTAAGGAAAATCGCCACCAGCATCAGCTGCATCAGCAGATTTCCATTTTCGGAGTTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCCAGGACGCCTAGAACCGCTCGTCAACTTAAAACTCCAAGCTCACATCCTGATTCCGTTTCCACTTCTCCACTTGCTGCTAGCAAGTCACCGAGAGAGAGGAGTCCTAGGGTGGTGACTGACCGCAAGTCACCACGATCTCTGGCCACTGAGAGTAAAGTGCCTAGCAAAGTGGCTGAATTGGAATCACAGCTTTCTCAACTCCAGGAGGAACTCAAGAAGACAAGTGACCAACTGAGCGCATCTGAATCAGACAAAAGACAGGCTCAACAGGAGGCAGAAGAAGCAAAGAAACAACTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGCTCGAGCTCTCCAATTCGGAGGAAGATCGTGTTCAAGAACTGCACAAAATTTCTCAAGATCGTGATCGAGCATGGCAGTCTGAGCTTGAGGCCATTCAAAAGCAACATTCAATTGATGCTGCTTCATTGGCCTCTGCCATGAATGAAGTTCAGAGGCTCAAGGTCCAGTTGGAGACGGCGTCTGAATCTGAGGCAACCCAGAGTAAGCTTGCTGAGTCTGCACAAACTGAGATAGATTGTTTAAGAACCCAGCTATCAGAGACTCTCTCCCTAGTTGAAAAACTGAAAGATGAGCTTAGTTACTGTAAAGAATCTGAAGCTCAAGCTCTTGAAGTTGCAAGGAAAAATCATAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTGCAATCGGATGGAATTAAAGCCATTGACGCTTACAACTCTCTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAAATCAGCAAGAAGATTCAGAAAGGTTTGTTAGATTCTGAAGACAATGGTTTAGATCCTGAAGTCAAAGCAAAAGCTGCTGAGCAAAACGATGGAAAGGATGATATTAGTAAGGTAAAAACTGAGCTAACCGCTTTGAGATTAGAAGCAGATAAGTCAAAATCTGCATTGGAGGCAGCTGAGGCAAGATTCGAGGAGGAATATGTTCGTACAACATTGCAAATTAGAATTGCTCACGAACTTGTGGAACAAATGAAAGTAGAGTCATGTCAGAAAGAGGCCGAATTGGATGCAGAACTTAAGGAGACCAGGGCGAACTTAGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAACTGTGTAGCATTGTGGAGGAGAACAAGGAGCTCAACTCAGAGATAAGCAAAATGATACCAGTCGACAGGGAGTCCGAACTAGCAATGGAACTACAAAGGTTAGAGGCTGATATGGTGGAGCTGAAGAACAGGCTCTTGGAAAAGGAAACACAGCTGCAGGGTACAACAGAGGAAAATGATGCACTCAAGAAGGAAATTGAGAAGATCAATGAGGAAGCGGTTAGTATAGCAGAAACAACCAAAGCTACAGAGAAGGAGGCGCTGATGAAACTCGAGCACGCAACAGAAGAGGCAGATAAAAGTAGCCGAAGAGCGGCACGAGTGGCCGAGCAATTGGATGCTGCACAGGCTGCAAGCTCTGAAATGGAAGCTGAGCTGAGGAGGTTGAAAGTGCAAGCAGAGCAGTGGAGGAAGGCAGCTGAGGCAGCTGCTGCAATACTCTCAACTGGGAACAATGGGAAGATTGTTGATAAACTAGTTTGTTTGGAGAACAATTACCCTATGGGGTCACCATACTCGGAAGATCTTGAGGCGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAGAGCAGAAGAAAACTGGTTAC

Protein sequence

GRRVTVVPPYAPSQVHRVSPSLSHGSSAASTQKTRVSVISSEFVKENRHQHQLHQQISIFGVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLWKKEQKKTGY
Homology
BLAST of Carg12600 vs. NCBI nr
Match: KAG7017345.1 (Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 670/670 (100.00%), Postives = 670/670 (100.00%), Query Frame = 0

Query: 1   GRRVTVVPPYAPSQVHRVSPSLSHGSSAASTQKTRVSVISSEFVKENRHQHQLHQQISIF 60
           GRRVTVVPPYAPSQVHRVSPSLSHGSSAASTQKTRVSVISSEFVKENRHQHQLHQQISIF
Sbjct: 1   GRRVTVVPPYAPSQVHRVSPSLSHGSSAASTQKTRVSVISSEFVKENRHQHQLHQQISIF 60

Query: 61  GVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSL 120
           GVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSL
Sbjct: 61  GVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSL 120

Query: 121 ATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLE 180
           ATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLE
Sbjct: 121 ATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLE 180

Query: 181 ESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK 240
           ESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK
Sbjct: 181 ESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK 240

Query: 241 VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR 300
           VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR
Sbjct: 241 VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR 300

Query: 301 KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE 360
           KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE
Sbjct: 301 KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE 360

Query: 361 DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ 420
           DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ
Sbjct: 361 DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ 420

Query: 421 IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE 480
           IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE
Sbjct: 421 IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE 480

Query: 481 ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV 540
           ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV
Sbjct: 481 ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV 540

Query: 541 SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE 600
           SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE
Sbjct: 541 SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE 600

Query: 601 QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVL 660
           QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVL
Sbjct: 601 QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVL 660

Query: 661 WKKEQKKTGY 671
           WKKEQKKTGY
Sbjct: 661 WKKEQKKTGY 670

BLAST of Carg12600 vs. NCBI nr
Match: KAG6580589.1 (Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1062.0 bits (2745), Expect = 2.1e-306
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK
Sbjct: 188 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI
Sbjct: 308 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI
Sbjct: 368 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS
Sbjct: 428 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 616

BLAST of Carg12600 vs. NCBI nr
Match: XP_022934537.1 (interactor of constitutive active ROPs 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1045.4 bits (2702), Expect = 2.1e-301
Identity = 602/609 (98.85%), Postives = 604/609 (99.18%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESKVPSKVAELESQLSQLQEE KKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKVPSKVAELESQLSQLQEEQKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLETASESE+TQS LAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK
Sbjct: 188 QLETASESESTQSTLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI
Sbjct: 308 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI
Sbjct: 368 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEE VS
Sbjct: 428 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEVVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKA E EALMKLEHATEEADKS+RRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKAAE-EALMKLEHATEEADKSNRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 615

BLAST of Carg12600 vs. NCBI nr
Match: XP_022983518.1 (interactor of constitutive active ROPs 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.8e-295
Identity = 587/609 (96.39%), Postives = 596/609 (97.87%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQL+TPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLQTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESK PSKVAELESQLSQLQEELKKTSDQLSASESDKRQA+QEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKGPSKVAELESQLSQLQEELKKTSDQLSASESDKRQARQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQVLELS SEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVLELSTSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLET SESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEV R 
Sbjct: 188 QLETVSESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVVRT 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           N LDP+V AKAAE+NDG D+I +VKTELTA+RLEADKSKSALEAAEARFEEEYVRTTLQI
Sbjct: 308 NDLDPDVNAKAAEENDGNDEIDEVKTELTAMRLEADKSKSALEAAEARFEEEYVRTTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENK LNSEI
Sbjct: 368 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKVLNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIP+DRE ELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS
Sbjct: 428 SKMIPIDREFELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKA EKEALMKLEHATEEADKSSRRA RVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKAAEKEALMKLEHATEEADKSSRRATRVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDK+VCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKIVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 616

BLAST of Carg12600 vs. NCBI nr
Match: XP_023528745.1 (interactor of constitutive active ROPs 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1016.9 bits (2628), Expect = 7.9e-293
Identity = 584/609 (95.89%), Postives = 592/609 (97.21%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESK PSK+AELESQ SQLQEEL KTSDQLSASESDKRQA+QEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKGPSKMAELESQFSQLQEELNKTSDQLSASESDKRQARQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQV ELS SEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVFELSTSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLE ASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEV RK
Sbjct: 188 QLEMASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVVRK 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQI KKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQIGKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           N LDP+V AKAAE+NDG D+I +VKTELTA+RLEADKSKSALEAAEARFEEEYVR+TLQI
Sbjct: 308 NDLDPDVNAKAAEENDGNDEIDEVKTELTAMRLEADKSKSALEAAEARFEEEYVRSTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESC KEAELDAELKETRANLEQLRVDLKEKETQLCS VEENK LNSEI
Sbjct: 368 RIAHELVEQMKVESCLKEAELDAELKETRANLEQLRVDLKEKETQLCSTVEENKILNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS
Sbjct: 428 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKA EKEALMKLEHATEEADKSSRRA RVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKAAEKEALMKLEHATEEADKSSRRAVRVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 616

BLAST of Carg12600 vs. ExPASy Swiss-Prot
Match: Q9ZQC5 (Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICR2 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.1e-106
Identity = 291/629 (46.26%), Postives = 400/629 (63.59%), Query Frame = 0

Query: 63  GSSEVPQRKSP-RTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           GS EVPQ+KSP  TP+TAR+LKT  S P S   SP    ++P+ +SP+VV DR+SPR+  
Sbjct: 9   GSLEVPQKKSPASTPKTARKLKTSESDPVS---SPNTKIRTPKTQSPKVVADRRSPRTPV 68

Query: 123 TE--SKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKL 182
            E   K   K  EL SQ+SQLQEELKK  +QLSASE+ K++AQ +AEE K          
Sbjct: 69  NEIQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEETK---------- 128

Query: 183 EESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRL 242
               QQ++E++ SE+ R+ EL K+SQ+RD+AWQSELEA+Q+QH++D+A+L+S MNEVQ+L
Sbjct: 129 ----QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSSTMNEVQKL 188

Query: 243 KVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVA 302
           K QL   SESE             ++ LR +L+ETLSLVEKL+ EL   KE EAQA E+ 
Sbjct: 189 KAQL---SESE------------NVENLRMELNETLSLVEKLRGELFDAKEGEAQAHEIV 248

Query: 303 RKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDS 362
                Q E A   +E L+SDG+K  +A NSL+ ELEQSK++++SLE+ + +      L+ 
Sbjct: 249 SGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQ------LEE 308

Query: 363 EDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTL 422
           ED     E +  A   N     + ++K E+   R E  + KSA+E  E R+ EEY+++TL
Sbjct: 309 ED-----EARGNA---NGDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTL 368

Query: 423 QIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNS 482
           QIR A+E V+++K    Q+EAEL  ELK+T+A  + L   L +KE +L  +V+EN+ LNS
Sbjct: 369 QIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNS 428

Query: 483 EISKMIPV---------DRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKK 542
           +I +   V           E E   EL++LE+D++EL+  L++KE +LQ    + ++L+ 
Sbjct: 429 KIKEKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQSVMSQYESLRS 488

Query: 543 EIEKINEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEA 602
           E+E +       +E  KA + EAL KL   TEEADKS +RA    EQL AAQ  ++E+EA
Sbjct: 489 EMETMQ------SEKNKAID-EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNTELEA 548

Query: 603 ELRRLKVQAEQWRKAAEAAAAILSTGN-----NGKIVDKLVCLEN-----NYPMGSPY-- 662
           ELRRLKVQ +QWRKAAEAAA +LS GN     NGK V++   LE+     N  M SPY  
Sbjct: 549 ELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLESPLRRRNVNM-SPYMG 583

Query: 663 -SEDLEASPKKKNGNVLKKIGVLWKKEQK 667
            ++D  +SPKKKNG++LKKIGVL KK QK
Sbjct: 609 ETDDELSSPKKKNGSMLKKIGVLLKKSQK 583

BLAST of Carg12600 vs. ExPASy Swiss-Prot
Match: Q9LSS5 (Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3 PE=1 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.4e-88
Identity = 251/607 (41.35%), Postives = 382/607 (62.93%), Query Frame = 0

Query: 63  GSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLAT 122
           GS +VP++ S   PR AR LK  +  P+S S+   A +++P+++SP V+ +R+SPRS  +
Sbjct: 9   GSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NRRSPRSPVS 68

Query: 123 ESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEES 182
           E K PS++ ELE  +SQLQEELKK  DQ+S SE+ K+QA+QEAEE++KQL ++S+KLEES
Sbjct: 69  EKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSKLEES 128

Query: 183 QQQVLELSNSEE--DRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK 242
           Q Q +E S  EE  D+   L   S  ++  W+    A ++      A LA+  +E+++LK
Sbjct: 129 QNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEIRQLK 188

Query: 243 VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR 302
           +Q+E  + SEA   K AE   +E+  LR  L +TL  VE  +++L  C+ SEA+   +A 
Sbjct: 189 LQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETEALAT 248

Query: 303 KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE 362
           +   Q E AK AVE+L+SDG KA+++Y  +++ELEQSK+++  LE  ++ K+Q    D E
Sbjct: 249 ETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVN-KLQNNPADLE 308

Query: 363 DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ 422
           ++    E+  K  E +  + + +++  E+++LR E ++ ++ALEA++ + +E  V  + +
Sbjct: 309 NH----EILLKDYE-SLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 368

Query: 423 IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE 482
           +RI               +AEL +ELK  ++ +++L+  L +KET+L  I EE    + +
Sbjct: 369 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 428

Query: 483 ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV 542
           + K     +E ++  EL++L   +  LK  L++KET+LQ  ++EN+ LK +I K      
Sbjct: 429 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHK------ 488

Query: 543 SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE 602
                ++   ++A +KL  A EEADKSS++A RV EQL+A QA++SEME ELR+LKVQ+ 
Sbjct: 489 -----SETDVQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSN 548

Query: 603 QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEAS-PKKKNGNVLKKIGV 662
           QWRKAAEAA A+LS GNNGK        EN     SPYSED++    KKKNGNVLKKIGV
Sbjct: 549 QWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGNVLKKIGV 564

Query: 663 LWKKEQK 667
           LWKK QK
Sbjct: 609 LWKKPQK 564

BLAST of Carg12600 vs. ExPASy Swiss-Prot
Match: Q8VYU8 (Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5 PE=1 SV=2)

HSP 1 Score: 173.7 bits (439), Expect = 7.0e-42
Identity = 187/607 (30.81%), Postives = 277/607 (45.63%), Query Frame = 0

Query: 63  GSSEVPQRKSP-RTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           GS E+PQ+KSP   P+  R+LK     P    + P   + S + + P+VV DR+S R   
Sbjct: 9   GSLELPQKKSPLPAPKVVRRLK-----PSGAESDPKTKTIS-KTQIPKVVADRRSARIPL 68

Query: 123 TE--SKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKL 182
            E   K   ++ ELES +SQLQEELKK  ++L+ SE+ KR+AQ+EAE+AK QL D++A  
Sbjct: 69  NEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLMDINA-- 128

Query: 183 EESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRL 242
                       SE+ R++EL K+SQ+RD+ WQSELEA+Q+QH +D+ +L+SA+NEVQ+L
Sbjct: 129 ------------SEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAINEVQKL 188

Query: 243 KVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVA 302
           K             SKL ES                                        
Sbjct: 189 K-------------SKLFESES-------------------------------------- 248

Query: 303 RKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDS 362
                                            ELEQSK +++SLE+ + +++++  ++S
Sbjct: 249 ---------------------------------ELEQSKYEVRSLEKLV-RQLEEERVNS 308

Query: 363 EDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTL 422
            D+    EV+              ++K  +   R E  + KSA+EAAE R++EEY+++TL
Sbjct: 309 RDSSSSMEVE--------------ELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTL 368

Query: 423 QIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNS 482
           QIR A+E  E +K    Q+EAEL  EL  T+  +E LR +L EK                
Sbjct: 369 QIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV--------------- 396

Query: 483 EISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEA 542
                    +E E   +L++LE+D++E++  L++KE +LQ       A++K++E  N EA
Sbjct: 429 ---------KEDESTGDLKKLESDLMEVRGSLMDKEMELQIL---RSAMEKKVETANTEA 396

Query: 543 VSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQA 602
                                                           MEAEL+R+K+Q 
Sbjct: 489 ------------------------------------------------MEAELKRVKIQC 396

Query: 603 EQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGV 662
           EQWRKAAE AA+IL                         + D E +   +   +LKK GV
Sbjct: 549 EQWRKAAETAASIL-------------------------NNDEERTDSIETSKMLKKFGV 396

Query: 663 LWKKEQK 667
           L KK  K
Sbjct: 609 LLKKNHK 396

BLAST of Carg12600 vs. ExPASy Swiss-Prot
Match: F4I8B9 (Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g65010 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.6e-08
Identity = 159/610 (26.07%), Postives = 286/610 (46.89%), Query Frame = 0

Query: 63  GSSEVPQRK-SPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           G  E P+ K SP  PR +   K  +S  DS S SP+  ++   +RSP  V  + +P    
Sbjct: 8   GLMETPRSKPSPPPPRLS---KLSASKSDSNSASPVPNTRLSLDRSPPTVNSKPTPDRRP 67

Query: 123 TESKVPSKV-------AELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSD 182
           +    P KV        EL++QL+Q+QE+LKK  +Q+   + DK +A  + +E++K + +
Sbjct: 68  SRIPTPEKVHSRLVKGTELQTQLNQIQEDLKKADEQIELLKKDKAKAIDDLKESEKLVEE 127

Query: 183 MSAKLEE---SQQQVLELSNSEEDRVQELHKIS----QDRDRAWQSELEAIQKQHSIDAA 242
            + KL+E   +Q++  E    E+ R  EL +      Q +D   ++ELE+I+ QH++D +
Sbjct: 128 ANEKLKEALAAQKRAEESFEVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHALDIS 187

Query: 243 SLASAMNEVQRLKVQLETASESEATQSKLAESAQTEIDCLRTQLSETL-SLVEKLKDELS 302
           +L S   E+QR+K +L   ++++      AE A T+I  +  + +E L S + +LK  L 
Sbjct: 188 ALLSTTEELQRVKHELSMTADAKNKALSHAEEA-TKIAEIHAEKAEILASELGRLKALLG 247

Query: 303 YCKESEA-QALEVARKNHNQFETAKAAVEK---LQSDGIKAIDAYNSLSLELEQSK---A 362
             +E EA +  E+  K  ++ E  +  +EK   L+S   +       L ++LE +K   +
Sbjct: 248 SKEEKEAIEGNEIVSKLKSEIELLRGELEKVSILESSLKEQEGLVEQLKVDLEAAKMAES 307

Query: 363 QIQSLEEQISKKIQKGLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKS 422
              S  E+   K+ +         L+ EV+     ++   + +  V  +L  L     ++
Sbjct: 308 CTNSSVEEWKNKVHE---------LEKEVEESNRSKSSASESMESVMKQLAELNHVLHET 367

Query: 423 KSALEAAEARFE-------------EEYVRTTLQIRIAHELVEQMK--VESCQKEAELDA 482
           KS   A + + E             EEY R   Q+ IA E   +++  VES + E E+  
Sbjct: 368 KSDNAAQKEKIELLEKTIEAQRTDLEEYGR---QVCIAKEEASKLENLVESIKSELEISQ 427

Query: 483 ELKETRANLEQLRV----DLKEKETQLCSIVEENKELNSEISKMIPVDRESELAMELQRL 542
           E K    + E+       +L ++ T+L SI  E  ++  E SK    D ES L + LQ  
Sbjct: 428 EEKTRALDNEKAATSNIQNLLDQRTEL-SIELERCKVEEEKSKK---DMES-LTLALQEA 487

Query: 543 EADMVELKNRLLEKETQLQGTTEENDALK-------KEIEKINEEAVSIAETTKATEKEA 602
             +  E K  LL  + +L+    + D+LK       ++ EK+ E+A +  ++ K+T    
Sbjct: 488 STESSEAKATLLVCQEELKNCESQVDSLKLASKETNEKYEKMLEDARNEIDSLKSTVDSI 547

Query: 603 LMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQWRKAAEAAAAIL 624
             + E++    ++         ++ +   ++S E  + L  L  ++E+   A +   A L
Sbjct: 548 QNEFENSKAGWEQKELHLMGCVKKSEEENSSSQEEVSRLVNLLKESEEDACARKEEEASL 594

BLAST of Carg12600 vs. ExPASy Swiss-Prot
Match: Q9LFE4 (WEB family protein At5g16730, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g16730 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.5e-07
Identity = 155/602 (25.75%), Postives = 293/602 (48.67%), Query Frame = 0

Query: 70  RKSPRTPRTARQL--KTPSSHPDSVSTSPLAASKSPRERSP--RVVTDRKSPRSLATESK 129
           + SP TPR A++   K+ +S+ +S ST+   +  S    SP  +   +R+SP+      K
Sbjct: 21  KSSPATPRIAKRTVNKSETSNNNSPSTTTPHSRLSLDRSSPNSKSSVERRSPKLPTPPEK 80

Query: 130 VPSKVAELE--------SQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSA 189
             ++VA ++        ++LSQ++E+LKK ++++S+ E DK +A  E ++AKK+   ++ 
Sbjct: 81  SQARVAAVKGTESPQTTTRLSQIKEDLKKANERISSLEKDKAKALDELKQAKKEAEQVTL 140

Query: 190 KLEE---SQQQVLELSNSEEDRVQELH---KISQDRDRAWQSELEAIQKQHSIDAASLAS 249
           KL++   +Q+ V E  NSE ++ Q +    +  Q+ +   + ELE ++ QH+ D+A+L +
Sbjct: 141 KLDDALKAQKHVEE--NSEIEKFQAVEAGIEAVQNNEEELKKELETVKNQHASDSAALVA 200

Query: 250 AMNEVQRLKVQLETASESEA-------TQSKLAESAQTEIDCLRTQLSETLSL------- 309
              E++++  +L  A ++++         SK AE    ++D L ++L+   +L       
Sbjct: 201 VRQELEKINEELAAAFDAKSKALSQAEDASKTAEIHAEKVDILSSELTRLKALLDSTREK 260

Query: 310 --------VEKLKDELSYCKESEAQALEVARKNHNQFETAKAAVEKLQSDGIKA-IDAYN 369
                   V KL+DE+   K      LE AR    + +  +  VEKL  D   A +   N
Sbjct: 261 TAISDNEMVAKLEDEIVVLKRD----LESARGFEAEVKEKEMIVEKLNVDLEAAKMAESN 320

Query: 370 SLSLELE-QSKAQIQSLEEQI--SKKIQKGLLDSEDNGLDPEVKAKAAEQNDGKDDISKV 429
           + SL  E QSKA  + LEEQ+  + K+++    S ++ +  +++    + +D + +I+ +
Sbjct: 321 AHSLSNEWQSKA--KELEEQLEEANKLERSASVSLESVM-KQLEGSNDKLHDTETEITDL 380

Query: 430 KTELTALRLEADKSKSALEAAEARF---EEEYVRTTLQIRIAHELVEQMKVESCQKEAEL 489
           K  +  L     K K  LE +E R    EEE  +   +       VE++K E    + E 
Sbjct: 381 KERIVTLETTVAKQKEDLEVSEQRLGSVEEEVSKNEKE-------VEKLKSELETVKEEK 440

Query: 490 DAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEISKMIPVDRESELAMELQRLEA 549
           +  LK+ +    +++  L E++++L S +E +KE   +  K +       LA  L  + +
Sbjct: 441 NRALKKEQDATSRVQ-RLSEEKSKLLSDLESSKEEEEKSKKAM-----ESLASALHEVSS 500

Query: 550 DMVELKNRLL-----EKETQLQGTTEENDALKKEIEKINEEA-------VSIAETTK--- 608
           +  ELK +LL     E ETQ+        A  ++ E + +EA       VS  E TK   
Sbjct: 501 EGRELKEKLLSQGDHEYETQIDDLKLVIKATNEKYENMLDEARHEIDVLVSAVEQTKKHF 560

BLAST of Carg12600 vs. ExPASy TrEMBL
Match: A0A6J1F319 (interactor of constitutive active ROPs 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441683 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 1.0e-301
Identity = 602/609 (98.85%), Postives = 604/609 (99.18%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESKVPSKVAELESQLSQLQEE KKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKVPSKVAELESQLSQLQEEQKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLETASESE+TQS LAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK
Sbjct: 188 QLETASESESTQSTLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI
Sbjct: 308 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI
Sbjct: 368 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEE VS
Sbjct: 428 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEVVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKA E EALMKLEHATEEADKS+RRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKAAE-EALMKLEHATEEADKSNRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 615

BLAST of Carg12600 vs. ExPASy TrEMBL
Match: A0A6J1J7Z2 (interactor of constitutive active ROPs 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111482097 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.8e-295
Identity = 587/609 (96.39%), Postives = 596/609 (97.87%), Query Frame = 0

Query: 62  VGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 121
           VGSSEVPQRKSPRTPRTARQL+TPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA
Sbjct: 8   VGSSEVPQRKSPRTPRTARQLQTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 67

Query: 122 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 181
           TESK PSKVAELESQLSQLQEELKKTSDQLSASESDKRQA+QEAEEAKKQLSDMSAKLEE
Sbjct: 68  TESKGPSKVAELESQLSQLQEELKKTSDQLSASESDKRQARQEAEEAKKQLSDMSAKLEE 127

Query: 182 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 241
           SQQQVLELS SEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV
Sbjct: 128 SQQQVLELSTSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 187

Query: 242 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 301
           QLET SESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEV R 
Sbjct: 188 QLETVSESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVVRT 247

Query: 302 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 361
           NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED
Sbjct: 248 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 307

Query: 362 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 421
           N LDP+V AKAAE+NDG D+I +VKTELTA+RLEADKSKSALEAAEARFEEEYVRTTLQI
Sbjct: 308 NDLDPDVNAKAAEENDGNDEIDEVKTELTAMRLEADKSKSALEAAEARFEEEYVRTTLQI 367

Query: 422 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 481
           RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENK LNSEI
Sbjct: 368 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKVLNSEI 427

Query: 482 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 541
           SKMIP+DRE ELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS
Sbjct: 428 SKMIPIDREFELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 487

Query: 542 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 601
           IAETTKA EKEALMKLEHATEEADKSSRRA RVAEQLDAAQAASSEMEAELRRLKVQAEQ
Sbjct: 488 IAETTKAAEKEALMKLEHATEEADKSSRRATRVAEQLDAAQAASSEMEAELRRLKVQAEQ 547

Query: 602 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 661
           WRKAAEAAAAILSTGNNGKIVDK+VCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW
Sbjct: 548 WRKAAEAAAAILSTGNNGKIVDKIVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 607

Query: 662 KKEQKKTGY 671
           KKEQKKTGY
Sbjct: 608 KKEQKKTGY 616

BLAST of Carg12600 vs. ExPASy TrEMBL
Match: A0A6J1CX56 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015104 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 3.2e-247
Identity = 505/618 (81.72%), Postives = 560/618 (90.61%), Query Frame = 0

Query: 57  ISIFGVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKS 116
           +  F  GSSEVPQ+KSPRTPRTARQLK PSS+P+S+STSPLAASK+P+ERSPR VTDRKS
Sbjct: 34  LGCFRTGSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDRKS 93

Query: 117 PRSLATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMS 176
           PR LATESK PSKVAELESQLS+LQEELKK SDQLSASES KR+AQQEAEEAKKQLS+MS
Sbjct: 94  PRCLATESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSEMS 153

Query: 177 AKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEV 236
           AKLEESQQQVLELS SEEDRVQELHKISQDRD+AWQSELEA+QKQH++DAA+LASAMNEV
Sbjct: 154 AKLEESQQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMNEV 213

Query: 237 QRLKVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQAL 296
           QRLK QLE  SESEAT+S LAESAQ EI+ LRTQLSET+ LVEKLKDELSYCKESEAQAL
Sbjct: 214 QRLKAQLEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQAL 273

Query: 297 EVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGL 356
           EVARKN NQFETAKAAVEKLQSDG+KAI++YNSLS+ELEQSKAQIQSLE QIS  IQKGL
Sbjct: 274 EVARKNQNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQIS-MIQKGL 333

Query: 357 LDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVR 416
           +D   N LDP V AK AE+N G D+I+++KTELTA+++EADKSKSAL+AAE R+EEEYVR
Sbjct: 334 VDPMGNDLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYVR 393

Query: 417 TTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKE 476
           T LQIRIAHELVEQMKVESC+KEAELDAELK+ RAN+EQLRVDL EKETQLCS+VEENK 
Sbjct: 394 TALQIRIAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENKA 453

Query: 477 LNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIE--- 536
           LNSE SK   VDRES+LA EL+RLEADM+ELK RLL+KET+LQ T EEN+ALKKEIE   
Sbjct: 454 LNSETSKSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKME 513

Query: 537 ----KINEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEME 596
               KIN++AV++AETTKA E+EALMKL HATEEADKSSRRAARVAEQLDAAQAA+SEME
Sbjct: 514 MQTSKINDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEME 573

Query: 597 AELRRLKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDL-EASPKK 656
           AELRRLKVQA+QWRKAAEAAAAILSTGNNGK VD++V LENNYP+GSPYSEDL + SPKK
Sbjct: 574 AELRRLKVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKK 633

Query: 657 KNGNVLKKIGVLWKKEQK 667
           KNGN+LKKIGVLWKK QK
Sbjct: 634 KNGNMLKKIGVLWKKNQK 649

BLAST of Carg12600 vs. ExPASy TrEMBL
Match: A0A6J1CXH6 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015104 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 2.1e-246
Identity = 504/612 (82.35%), Postives = 558/612 (91.18%), Query Frame = 0

Query: 63  GSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLAT 122
           GSSEVPQ+KSPRTPRTARQLK PSS+P+S+STSPLAASK+P+ERSPR VTDRKSPR LAT
Sbjct: 9   GSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDRKSPRCLAT 68

Query: 123 ESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEES 182
           ESK PSKVAELESQLS+LQEELKK SDQLSASES KR+AQQEAEEAKKQLS+MSAKLEES
Sbjct: 69  ESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSEMSAKLEES 128

Query: 183 QQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKVQ 242
           QQQVLELS SEEDRVQELHKISQDRD+AWQSELEA+QKQH++DAA+LASAMNEVQRLK Q
Sbjct: 129 QQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMNEVQRLKAQ 188

Query: 243 LETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARKN 302
           LE  SESEAT+S LAESAQ EI+ LRTQLSET+ LVEKLKDELSYCKESEAQALEVARKN
Sbjct: 189 LEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQALEVARKN 248

Query: 303 HNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSEDN 362
            NQFETAKAAVEKLQSDG+KAI++YNSLS+ELEQSKAQIQSLE QIS  IQKGL+D   N
Sbjct: 249 QNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQIS-MIQKGLVDPMGN 308

Query: 363 GLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQIR 422
            LDP V AK AE+N G D+I+++KTELTA+++EADKSKSAL+AAE R+EEEYVRT LQIR
Sbjct: 309 DLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYVRTALQIR 368

Query: 423 IAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEIS 482
           IAHELVEQMKVESC+KEAELDAELK+ RAN+EQLRVDL EKETQLCS+VEENK LNSE S
Sbjct: 369 IAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENKALNSETS 428

Query: 483 KMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIE-------KI 542
           K   VDRES+LA EL+RLEADM+ELK RLL+KET+LQ T EEN+ALKKEIE       KI
Sbjct: 429 KSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKMEMQTSKI 488

Query: 543 NEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRL 602
           N++AV++AETTKA E+EALMKL HATEEADKSSRRAARVAEQLDAAQAA+SEMEAELRRL
Sbjct: 489 NDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEMEAELRRL 548

Query: 603 KVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDL-EASPKKKNGNVL 662
           KVQA+QWRKAAEAAAAILSTGNNGK VD++V LENNYP+GSPYSEDL + SPKKKNGN+L
Sbjct: 549 KVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNML 608

Query: 663 KKIGVLWKKEQK 667
           KKIGVLWKK QK
Sbjct: 609 KKIGVLWKKNQK 618

BLAST of Carg12600 vs. ExPASy TrEMBL
Match: A0A6J1F7Y3 (interactor of constitutive active ROPs 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441683 PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 1.3e-245
Identity = 490/496 (98.79%), Postives = 492/496 (99.19%), Query Frame = 0

Query: 175 MSAKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN 234
           MSAKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN
Sbjct: 1   MSAKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN 60

Query: 235 EVQRLKVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ 294
           EVQRLKVQLETASESE+TQS LAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ
Sbjct: 61  EVQRLKVQLETASESESTQSTLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ 120

Query: 295 ALEVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK 354
           ALEVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK
Sbjct: 121 ALEVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK 180

Query: 355 GLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEY 414
           GLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEY
Sbjct: 181 GLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEY 240

Query: 415 VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN 474
           VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN
Sbjct: 241 VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN 300

Query: 475 KELNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEK 534
           KELNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEK
Sbjct: 301 KELNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEK 360

Query: 535 INEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRR 594
           INEE VSIAETTKA E EALMKLEHATEEADKS+RRAARVAEQLDAAQAASSEMEAELRR
Sbjct: 361 INEEVVSIAETTKAAE-EALMKLEHATEEADKSNRRAARVAEQLDAAQAASSEMEAELRR 420

Query: 595 LKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVL 654
           LKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVL
Sbjct: 421 LKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVL 480

Query: 655 KKIGVLWKKEQKKTGY 671
           KKIGVLWKKEQKKTGY
Sbjct: 481 KKIGVLWKKEQKKTGY 495

BLAST of Carg12600 vs. TAIR 10
Match: AT2G37080.1 (ROP interactive partner 3 )

HSP 1 Score: 387.5 bits (994), Expect = 2.2e-107
Identity = 291/629 (46.26%), Postives = 400/629 (63.59%), Query Frame = 0

Query: 63  GSSEVPQRKSP-RTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           GS EVPQ+KSP  TP+TAR+LKT  S P S   SP    ++P+ +SP+VV DR+SPR+  
Sbjct: 9   GSLEVPQKKSPASTPKTARKLKTSESDPVS---SPNTKIRTPKTQSPKVVADRRSPRTPV 68

Query: 123 TE--SKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKL 182
            E   K   K  EL SQ+SQLQEELKK  +QLSASE+ K++AQ +AEE K          
Sbjct: 69  NEIQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEETK---------- 128

Query: 183 EESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRL 242
               QQ++E++ SE+ R+ EL K+SQ+RD+AWQSELEA+Q+QH++D+A+L+S MNEVQ+L
Sbjct: 129 ----QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSSTMNEVQKL 188

Query: 243 KVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVA 302
           K QL   SESE             ++ LR +L+ETLSLVEKL+ EL   KE EAQA E+ 
Sbjct: 189 KAQL---SESE------------NVENLRMELNETLSLVEKLRGELFDAKEGEAQAHEIV 248

Query: 303 RKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDS 362
                Q E A   +E L+SDG+K  +A NSL+ ELEQSK++++SLE+ + +      L+ 
Sbjct: 249 SGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQ------LEE 308

Query: 363 EDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTL 422
           ED     E +  A   N     + ++K E+   R E  + KSA+E  E R+ EEY+++TL
Sbjct: 309 ED-----EARGNA---NGDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTL 368

Query: 423 QIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNS 482
           QIR A+E V+++K    Q+EAEL  ELK+T+A  + L   L +KE +L  +V+EN+ LNS
Sbjct: 369 QIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNS 428

Query: 483 EISKMIPV---------DRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKK 542
           +I +   V           E E   EL++LE+D++EL+  L++KE +LQ    + ++L+ 
Sbjct: 429 KIKEKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQSVMSQYESLRS 488

Query: 543 EIEKINEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEA 602
           E+E +       +E  KA + EAL KL   TEEADKS +RA    EQL AAQ  ++E+EA
Sbjct: 489 EMETMQ------SEKNKAID-EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNTELEA 548

Query: 603 ELRRLKVQAEQWRKAAEAAAAILSTGN-----NGKIVDKLVCLEN-----NYPMGSPY-- 662
           ELRRLKVQ +QWRKAAEAAA +LS GN     NGK V++   LE+     N  M SPY  
Sbjct: 549 ELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLESPLRRRNVNM-SPYMG 583

Query: 663 -SEDLEASPKKKNGNVLKKIGVLWKKEQK 667
            ++D  +SPKKKNG++LKKIGVL KK QK
Sbjct: 609 ETDDELSSPKKKNGSMLKKIGVLLKKSQK 583

BLAST of Carg12600 vs. TAIR 10
Match: AT5G60210.1 (ROP interactive partner 5 )

HSP 1 Score: 326.6 bits (836), Expect = 4.6e-89
Identity = 251/607 (41.35%), Postives = 382/607 (62.93%), Query Frame = 0

Query: 63  GSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLAT 122
           GS +VP++ S   PR AR LK  +  P+S S+   A +++P+++SP V+ +R+SPRS  +
Sbjct: 9   GSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NRRSPRSPVS 68

Query: 123 ESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEES 182
           E K PS++ ELE  +SQLQEELKK  DQ+S SE+ K+QA+QEAEE++KQL ++S+KLEES
Sbjct: 69  EKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSKLEES 128

Query: 183 QQQVLELSNSEE--DRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK 242
           Q Q +E S  EE  D+   L   S  ++  W+    A ++      A LA+  +E+++LK
Sbjct: 129 QNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEIRQLK 188

Query: 243 VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR 302
           +Q+E  + SEA   K AE   +E+  LR  L +TL  VE  +++L  C+ SEA+   +A 
Sbjct: 189 LQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETEALAT 248

Query: 303 KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE 362
           +   Q E AK AVE+L+SDG KA+++Y  +++ELEQSK+++  LE  ++ K+Q    D E
Sbjct: 249 ETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVN-KLQNNPADLE 308

Query: 363 DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ 422
           ++    E+  K  E +  + + +++  E+++LR E ++ ++ALEA++ + +E  V  + +
Sbjct: 309 NH----EILLKDYE-SLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 368

Query: 423 IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE 482
           +RI               +AEL +ELK  ++ +++L+  L +KET+L  I EE    + +
Sbjct: 369 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 428

Query: 483 ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV 542
           + K     +E ++  EL++L   +  LK  L++KET+LQ  ++EN+ LK +I K      
Sbjct: 429 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHK------ 488

Query: 543 SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE 602
                ++   ++A +KL  A EEADKSS++A RV EQL+A QA++SEME ELR+LKVQ+ 
Sbjct: 489 -----SETDVQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSN 548

Query: 603 QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEAS-PKKKNGNVLKKIGV 662
           QWRKAAEAA A+LS GNNGK        EN     SPYSED++    KKKNGNVLKKIGV
Sbjct: 549 QWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGNVLKKIGV 564

Query: 663 LWKKEQK 667
           LWKK QK
Sbjct: 609 LWKKPQK 564

BLAST of Carg12600 vs. TAIR 10
Match: AT5G60210.2 (ROP interactive partner 5 )

HSP 1 Score: 326.6 bits (836), Expect = 4.6e-89
Identity = 251/607 (41.35%), Postives = 382/607 (62.93%), Query Frame = 0

Query: 63  GSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLAT 122
           GS +VP++ S   PR AR LK  +  P+S S+   A +++P+++SP V+ +R+SPRS  +
Sbjct: 9   GSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NRRSPRSPVS 68

Query: 123 ESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEES 182
           E K PS++ ELE  +SQLQEELKK  DQ+S SE+ K+QA+QEAEE++KQL ++S+KLEES
Sbjct: 69  EKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSKLEES 128

Query: 183 QQQVLELSNSEE--DRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLK 242
           Q Q +E S  EE  D+   L   S  ++  W+    A ++      A LA+  +E+++LK
Sbjct: 129 QNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEIRQLK 188

Query: 243 VQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVAR 302
           +Q+E  + SEA   K AE   +E+  LR  L +TL  VE  +++L  C+ SEA+   +A 
Sbjct: 189 LQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETEALAT 248

Query: 303 KNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSE 362
           +   Q E AK AVE+L+SDG KA+++Y  +++ELEQSK+++  LE  ++ K+Q    D E
Sbjct: 249 ETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVN-KLQNNPADLE 308

Query: 363 DNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQ 422
           ++    E+  K  E +  + + +++  E+++LR E ++ ++ALEA++ + +E  V  + +
Sbjct: 309 NH----EILLKDYE-SLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 368

Query: 423 IRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSE 482
           +RI               +AEL +ELK  ++ +++L+  L +KET+L  I EE    + +
Sbjct: 369 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 428

Query: 483 ISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAV 542
           + K     +E ++  EL++L   +  LK  L++KET+LQ  ++EN+ LK +I K      
Sbjct: 429 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHK------ 488

Query: 543 SIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAE 602
                ++   ++A +KL  A EEADKSS++A RV EQL+A QA++SEME ELR+LKVQ+ 
Sbjct: 489 -----SETDVQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSN 548

Query: 603 QWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEAS-PKKKNGNVLKKIGV 662
           QWRKAAEAA A+LS GNNGK        EN     SPYSED++    KKKNGNVLKKIGV
Sbjct: 549 QWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGNVLKKIGV 564

Query: 663 LWKKEQK 667
           LWKK QK
Sbjct: 609 LWKKPQK 564

BLAST of Carg12600 vs. TAIR 10
Match: AT3G53350.2 (ROP interactive partner 4 )

HSP 1 Score: 178.7 bits (452), Expect = 1.5e-44
Identity = 187/605 (30.91%), Postives = 277/605 (45.79%), Query Frame = 0

Query: 63  GSSEVPQRKSP-RTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           GS E+PQ+KSP   P+  R+LK     P    + P   + S + + P+VV DR+S R   
Sbjct: 9   GSLELPQKKSPLPAPKVVRRLK-----PSGAESDPKTKTIS-KTQIPKVVADRRSARIPL 68

Query: 123 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 182
            E K   ++ ELES +SQLQEELKK  ++L+ SE+ KR+AQ+EAE+AK QL D++A    
Sbjct: 69  NEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLMDINA---- 128

Query: 183 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 242
                     SE+ R++EL K+SQ+RD+ WQSELEA+Q+QH +D+ +L+SA+NEVQ+LK 
Sbjct: 129 ----------SEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAINEVQKLK- 188

Query: 243 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 302
                       SKL ES                                          
Sbjct: 189 ------------SKLFESES---------------------------------------- 248

Query: 303 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 362
                                          ELEQSK +++SLE+ + +++++  ++S D
Sbjct: 249 -------------------------------ELEQSKYEVRSLEKLV-RQLEEERVNSRD 308

Query: 363 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 422
           +    EV+              ++K  +   R E  + KSA+EAAE R++EEY+++TLQI
Sbjct: 309 SSSSMEVE--------------ELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQI 368

Query: 423 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 482
           R A+E  E +K    Q+EAEL  EL  T+  +E LR +L EK                  
Sbjct: 369 RSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV----------------- 394

Query: 483 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 542
                  +E E   +L++LE+D++E++  L++KE +LQ       A++K++E  N EA  
Sbjct: 429 -------KEDESTGDLKKLESDLMEVRGSLMDKEMELQIL---RSAMEKKVETANTEA-- 394

Query: 543 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 602
                                                         MEAEL+R+K+Q EQ
Sbjct: 489 ----------------------------------------------MEAELKRVKIQCEQ 394

Query: 603 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 662
           WRKAAE AA+IL                         + D E +   +   +LKK GVL 
Sbjct: 549 WRKAAETAASIL-------------------------NNDEERTDSIETSKMLKKFGVLL 394

Query: 663 KKEQK 667
           KK  K
Sbjct: 609 KKNHK 394

BLAST of Carg12600 vs. TAIR 10
Match: AT3G53350.3 (ROP interactive partner 4 )

HSP 1 Score: 178.7 bits (452), Expect = 1.5e-44
Identity = 187/605 (30.91%), Postives = 277/605 (45.79%), Query Frame = 0

Query: 63  GSSEVPQRKSP-RTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDRKSPRSLA 122
           GS E+PQ+KSP   P+  R+LK     P    + P   + S + + P+VV DR+S R   
Sbjct: 9   GSLELPQKKSPLPAPKVVRRLK-----PSGAESDPKTKTIS-KTQIPKVVADRRSARIPL 68

Query: 123 TESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSDMSAKLEE 182
            E K   ++ ELES +SQLQEELKK  ++L+ SE+ KR+AQ+EAE+AK QL D++A    
Sbjct: 69  NEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLMDINA---- 128

Query: 183 SQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMNEVQRLKV 242
                     SE+ R++EL K+SQ+RD+ WQSELEA+Q+QH +D+ +L+SA+NEVQ+LK 
Sbjct: 129 ----------SEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAINEVQKLK- 188

Query: 243 QLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQALEVARK 302
                       SKL ES                                          
Sbjct: 189 ------------SKLFESES---------------------------------------- 248

Query: 303 NHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQKGLLDSED 362
                                          ELEQSK +++SLE+ + +++++  ++S D
Sbjct: 249 -------------------------------ELEQSKYEVRSLEKLV-RQLEEERVNSRD 308

Query: 363 NGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEYVRTTLQI 422
           +    EV+              ++K  +   R E  + KSA+EAAE R++EEY+++TLQI
Sbjct: 309 SSSSMEVE--------------ELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQI 368

Query: 423 RIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEENKELNSEI 482
           R A+E  E +K    Q+EAEL  EL  T+  +E LR +L EK                  
Sbjct: 369 RSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV----------------- 394

Query: 483 SKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIEKINEEAVS 542
                  +E E   +L++LE+D++E++  L++KE +LQ       A++K++E  N EA  
Sbjct: 429 -------KEDESTGDLKKLESDLMEVRGSLMDKEMELQIL---RSAMEKKVETANTEA-- 394

Query: 543 IAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSEMEAELRRLKVQAEQ 602
                                                         MEAEL+R+K+Q EQ
Sbjct: 489 ----------------------------------------------MEAELKRVKIQCEQ 394

Query: 603 WRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDLEASPKKKNGNVLKKIGVLW 662
           WRKAAE AA+IL                         + D E +   +   +LKK GVL 
Sbjct: 549 WRKAAETAASIL-------------------------NNDEERTDSIETSKMLKKFGVLL 394

Query: 663 KKEQK 667
           KK  K
Sbjct: 609 KKNHK 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017345.10.0e+00100.00Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argy... [more]
KAG6580589.12.1e-306100.00Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argy... [more]
XP_022934537.12.1e-30198.85interactor of constitutive active ROPs 2, chloroplastic isoform X1 [Cucurbita mo... [more]
XP_022983518.13.8e-29596.39interactor of constitutive active ROPs 2, chloroplastic [Cucurbita maxima][more]
XP_023528745.17.9e-29395.89interactor of constitutive active ROPs 2, chloroplastic-like [Cucurbita pepo sub... [more]
Match NameE-valueIdentityDescription
Q9ZQC53.1e-10646.26Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana ... [more]
Q9LSS56.4e-8841.35Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3... [more]
Q8VYU87.0e-4230.81Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5... [more]
F4I8B98.6e-0826.07Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9LFE49.5e-0725.75WEB family protein At5g16730, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Match NameE-valueIdentityDescription
A0A6J1F3191.0e-30198.85interactor of constitutive active ROPs 2, chloroplastic isoform X1 OS=Cucurbita ... [more]
A0A6J1J7Z21.8e-29596.39interactor of constitutive active ROPs 2, chloroplastic OS=Cucurbita maxima OX=3... [more]
A0A6J1CX563.2e-24781.72interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 OS=Momor... [more]
A0A6J1CXH62.1e-24682.35interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 OS=Momor... [more]
A0A6J1F7Y31.3e-24598.79interactor of constitutive active ROPs 2, chloroplastic isoform X2 OS=Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT2G37080.12.2e-10746.26ROP interactive partner 3 [more]
AT5G60210.14.6e-8941.35ROP interactive partner 5 [more]
AT5G60210.24.6e-8941.35ROP interactive partner 5 [more]
AT3G53350.21.5e-4430.91ROP interactive partner 4 [more]
AT3G53350.31.5e-4430.91ROP interactive partner 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 229..249
NoneNo IPR availableCOILSCoilCoilcoord: 389..409
NoneNo IPR availableCOILSCoilCoilcoord: 439..466
NoneNo IPR availableCOILSCoilCoilcoord: 130..192
NoneNo IPR availableCOILSCoilCoilcoord: 557..605
NoneNo IPR availableCOILSCoilCoilcoord: 327..354
NoneNo IPR availableCOILSCoilCoilcoord: 490..545
NoneNo IPR availableCOILSCoilCoilcoord: 264..284
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..183
NoneNo IPR availablePANTHERPTHR34224:SF4INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTICcoord: 62..666
IPR029688Interactor of constitutive active ROPsPANTHERPTHR34224INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATEDcoord: 62..666

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12600-RACarg12600-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001682 tRNA 5'-leader removal
cellular_component GO:0030677 ribonuclease P complex
molecular_function GO:0003723 RNA binding