Carg12559 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12559
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr14: 625788 .. 626351 (-)
RNA-Seq Expression	Carg12559
Synteny	Carg12559

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCAAATGGACACCAGCGCCGCCCTGATGAATCGATCGACGCCGCCGCACCCGACGCCACATCGGACTAACGATTTCTCTCCAATTGAATTCATTCTCGGCCTCATCGCCGTCATCGCCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCAGGTCTCCTCCGCCTCTGGCGCCGGAGATCCGCTAGGCTCTCCGGTGCTCCTGTCACCGTTGTAGCGGGAAATCGTTCTTCTTATAGTGATGTGGCTGTGACCTTTAAGTACAACAAGGACGAGGTTGGTAAGGAAGTCGGCAACGAATGTCCTGTATGCTTGTCGGCGTTCGTTGACGGCGAGGAGATCCGGCAACTCATCGCCTGTAAACACTCTTTTCATGTGGATTGCATCGATATGTGGTTCTACTCACACCCTAATTGCCCGGTTTGTCGCGCCGAGATCGCCATCGCTGTTAAGCGGCCGATTAATAACCGCGCGCCGCGGCGACCGGCGGCTTCTAGAAGCGACGATTTCCATCAAGGACTGCCGGATGCGGGAAATTTAGTTTGA

mRNA sequence

ATGTCCCAAATGGACACCAGCGCCGCCCTGATGAATCGATCGACGCCGCCGCACCCGACGCCACATCGGACTAACGATTTCTCTCCAATTGAATTCATTCTCGGCCTCATCGCCGTCATCGCCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCAGGTCTCCTCCGCCTCTGGCGCCGGAGATCCGCTAGGCTCTCCGGTGCTCCTGTCACCGTTGTAGCGGGAAATCGTTCTTCTTATAGTGATGTGGCTGTGACCTTTAAGTACAACAAGGACGAGGTTGGTAAGGAAGTCGGCAACGAATGTCCTGTATGCTTGTCGGCGTTCGTTGACGGCGAGGAGATCCGGCAACTCATCGCCTGTAAACACTCTTTTCATGTGGATTGCATCGATATGTGGTTCTACTCACACCCTAATTGCCCGGTTTGTCGCGCCGAGATCGCCATCGCTGTTAAGCGGCCGATTAATAACCGCGCGCCGCGGCGACCGGCGGCTTCTAGAAGCGACGATTTCCATCAAGGACTGCCGGATGCGGGAAATTTAGTTTGA

Coding sequence (CDS)

ATGTCCCAAATGGACACCAGCGCCGCCCTGATGAATCGATCGACGCCGCCGCACCCGACGCCACATCGGACTAACGATTTCTCTCCAATTGAATTCATTCTCGGCCTCATCGCCGTCATCGCCATCCCCGCCCTTGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCAGGTCTCCTCCGCCTCTGGCGCCGGAGATCCGCTAGGCTCTCCGGTGCTCCTGTCACCGTTGTAGCGGGAAATCGTTCTTCTTATAGTGATGTGGCTGTGACCTTTAAGTACAACAAGGACGAGGTTGGTAAGGAAGTCGGCAACGAATGTCCTGTATGCTTGTCGGCGTTCGTTGACGGCGAGGAGATCCGGCAACTCATCGCCTGTAAACACTCTTTTCATGTGGATTGCATCGATATGTGGTTCTACTCACACCCTAATTGCCCGGTTTGTCGCGCCGAGATCGCCATCGCTGTTAAGCGGCCGATTAATAACCGCGCGCCGCGGCGACCGGCGGCTTCTAGAAGCGACGATTTCCATCAAGGACTGCCGGATGCGGGAAATTTAGTTTGA

Protein sequence

MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDAGNLV

Homology

[Close]

BLAST of Carg12559 vs. NCBI nr
Match: XP_022934546.1 (RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017306.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.0 bits (998), Expect = 2.2e-104
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60
           MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL
Sbjct: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60

Query: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120
           RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI
Sbjct: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120

Query: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180
           RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL
Sbjct: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180

Query: 181 PDAGNLV 188
           PDAGNLV
Sbjct: 181 PDAGNLV 187

[Close]

BLAST of Carg12559 vs. NCBI nr
Match: XP_023528143.1 (RING-H2 finger protein ATL33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.6 bits (984), Expect = 9.4e-103
Identity = 185/187 (98.93%), Postives = 185/187 (98.93%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60
           MSQMDTSAALMNRSTPPHPT HRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL
Sbjct: 1   MSQMDTSAALMNRSTPPHPTTHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60

Query: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120
           RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI
Sbjct: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120

Query: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180
           RQLIACKHSFHVDCIDMW YSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL
Sbjct: 121 RQLIACKHSFHVDCIDMWLYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180

Query: 181 PDAGNLV 188
           PDAGNLV
Sbjct: 181 PDAGNLV 187

[Close]

BLAST of Carg12559 vs. NCBI nr
Match: XP_022983254.1 (RING-H2 finger protein ATL33-like [Cucurbita maxima])

HSP 1 Score: 375.2 bits (962), Expect = 3.4e-100
Identity = 179/187 (95.72%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60
           MSQMD+SAALMNRSTPPHP  HRTNDFSP EFILGL+AVIAIPALVYSFFFAIKCPPGLL
Sbjct: 1   MSQMDSSAALMNRSTPPHPMTHRTNDFSPFEFILGLVAVIAIPALVYSFFFAIKCPPGLL 60

Query: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120
           RLWRRRSARLSGAPV VVAGNRSSYSDVA TFKYNKDEVGKEVGNECPVCLSAFVDGEEI
Sbjct: 61  RLWRRRSARLSGAPVAVVAGNRSSYSDVAATFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120

Query: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180
           RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEI IAVKRPINNRAPRRPAASRSDDFHQGL
Sbjct: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIVIAVKRPINNRAPRRPAASRSDDFHQGL 180

Query: 181 PDAGNLV 188
           PDAGNLV
Sbjct: 181 PDAGNLV 187

[Close]

BLAST of Carg12559 vs. NCBI nr
Match: XP_038905078.1 (RING-H2 finger protein ATL33 [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 7.0e-82
Identity = 152/184 (82.61%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63
           MD SA+LMNRS PP PTPHRT DFSPIEFILGL+AVI IPALVYSFFFAIKCPP LLRLW
Sbjct: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60

Query: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123
           R RSARLSGA VTVV  NR+  SDVA   KYNKDE  KEV NECPVCLSAF DGEEIRQL
Sbjct: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120

Query: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183
            ACKHSFHVDCIDMW YSHPNCPVCRA + +AVKRPINN APRRPA SRSDDF QGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180

Query: 184 GNLV 188
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Carg12559 vs. NCBI nr
Match: KAE8651013.1 (hypothetical protein Csa_001104 [Cucumis sativus])

HSP 1 Score: 311.6 bits (797), Expect = 4.6e-81
Identity = 148/186 (79.57%), Postives = 157/186 (84.41%), Query Frame = 0


Query: 2   SQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLR 61
           S MD +A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPALVYSF FAIKCPP  LR
Sbjct: 40  SAMDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLR 99

Query: 62  LWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIR 121
           LWR+RS RLSGA  TVV GNR+  SDVA   KYNKDE  KEVGNECPVCLSAF DGEEIR
Sbjct: 100 LWRQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIR 159

Query: 122 QLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLP 181
           QL  CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPI+N APRRPAASRSDDFHQGLP
Sbjct: 160 QLAVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLP 219

Query: 182 DAGNLV 188
           DAG LV
Sbjct: 220 DAGYLV 225

[Close]

BLAST of Carg12559 vs. ExPASy Swiss-Prot
Match: O80927 (RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 78/190 (41.05%), Postives = 107/190 (56.32%), Query Frame = 0


Query: 16  PPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLWRRRSARLS-GAP 75
           PP P P R+ D +P++ I  +IA +A+PALVY+ FF   C          R++  S   P
Sbjct: 48  PPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSSRRNSSSSRTSSSSDDTP 107

Query: 76  VTVVAGNRSSYSDVAVTF----KYNKDEVGKEVGNECPVCLSAFVDGEEIRQLIACKHSF 135
              V  +    ++  VT     K++KD   KE+GNEC VCL  F D +E+RQL  CKH+F
Sbjct: 108 HATV--DTPPITETTVTSESGGKFHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHAF 167

Query: 136 HVDCIDMWFYSHPNCPVCRAEIAI-----AVKRPIN-----NRA---PRRPAASRSDDFH 188
           HV CI+ W   HPNCP+CR ++++     A   P+N     NR+    R  A SR DD+ 
Sbjct: 168 HVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDWR 227

[Close]

BLAST of Carg12559 vs. ExPASy Swiss-Prot
Match: Q9XF63 (RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.5e-13
Identity = 40/95 (42.11%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 56  PPGLLRLWRRRSARLSGAPVTVVAGNRS-SYSDVAVTFKYNKDEVGKEVGNECPVCLSAF 115
           PP + R  RRR   + G       G  S   S + + F + +D    + G EC +CLS  
Sbjct: 78  PPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVF-FRQDSC--KDGLECSICLSEL 137

Query: 116 VDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCR 150
           V G++ R L  C HSFHV+CIDMWF SH  CP+CR
Sbjct: 138 VKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICR 169

[Close]

BLAST of Carg12559 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.4e-13
Identity = 58/180 (32.22%), Postives = 80/180 (44.44%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPI---------EFILGLIAVIAIPA----LVY 60
           M   D      N   PP P P  T   SPI          F +  IAVI I A    LV 
Sbjct: 1   MDLTDRRNPFNNLVFPPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVS 60

Query: 61  SFFFAIKCPPG--LLRLWRRRSARLSGAPVTVVA------GNRSSYSDVAVTFKYNKDEV 120
            + F IKC      + ++RRR       P+ + +      G   S       FK+ K +V
Sbjct: 61  YYIFVIKCCLNWHQIDIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDV 120

Query: 121 --GKE----VGNECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIA 154
             G+E       EC VCL+ F + E++R +  C H FH+DCID+W   + NCP+CR  ++
Sbjct: 121 VAGEEDQSKNSQECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

[Close]

BLAST of Carg12559 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.4e-13
Identity = 38/109 (34.86%), Postives = 57/109 (52.29%), Query Frame = 0


Query: 75  VTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQLIACKHSFHVDC 134
           +  V  +RS+ + + V   + K E G   G EC VCL+ F + E +R L  C H+FH++C
Sbjct: 105 IPTVGLHRSAINSITVV-GFKKGE-GIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNC 164

Query: 135 IDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 184
           ID W  SH NCP+CRA + +  + P           S S +  +G  D+
Sbjct: 165 IDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSESSNDLRGRQDS 211

[Close]

BLAST of Carg12559 vs. ExPASy Swiss-Prot
Match: P0C035 (RING-H2 finger protein ATL60 OS=Arabidopsis thaliana OX=3702 GN=ATL60 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.4e-13
Identity = 30/46 (65.22%), Postives = 33/46 (71.74%), Query Frame = 0


Query: 104 GNECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCR 150
           G EC VCLS  VDG++ R L  C H FHVDCIDMWF SH  CP+CR
Sbjct: 117 GLECAVCLSDLVDGDKARVLPRCNHGFHVDCIDMWFQSHSTCPLCR 162

[Close]

BLAST of Carg12559 vs. ExPASy TrEMBL
Match: A0A6J1F238 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.1e-104
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60
           MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL
Sbjct: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60

Query: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120
           RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI
Sbjct: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120

Query: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180
           RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL
Sbjct: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180

Query: 181 PDAGNLV 188
           PDAGNLV
Sbjct: 181 PDAGNLV 187

[Close]

BLAST of Carg12559 vs. ExPASy TrEMBL
Match: A0A6J1J6T6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 PE=3 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.6e-100
Identity = 179/187 (95.72%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLL 60
           MSQMD+SAALMNRSTPPHP  HRTNDFSP EFILGL+AVIAIPALVYSFFFAIKCPPGLL
Sbjct: 1   MSQMDSSAALMNRSTPPHPMTHRTNDFSPFEFILGLVAVIAIPALVYSFFFAIKCPPGLL 60

Query: 61  RLWRRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120
           RLWRRRSARLSGAPV VVAGNRSSYSDVA TFKYNKDEVGKEVGNECPVCLSAFVDGEEI
Sbjct: 61  RLWRRRSARLSGAPVAVVAGNRSSYSDVAATFKYNKDEVGKEVGNECPVCLSAFVDGEEI 120

Query: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGL 180
           RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEI IAVKRPINNRAPRRPAASRSDDFHQGL
Sbjct: 121 RQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIVIAVKRPINNRAPRRPAASRSDDFHQGL 180

Query: 181 PDAGNLV 188
           PDAGNLV
Sbjct: 181 PDAGNLV 187

[Close]

BLAST of Carg12559 vs. ExPASy TrEMBL
Match: A0A0A0LA67 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 4.9e-81
Identity = 147/184 (79.89%), Postives = 156/184 (84.78%), Query Frame = 0


Query: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63
           MD +A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPALVYSF FAIKCPP  LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123
           R+RS RLSGA  TVV GNR+  SDVA   KYNKDE  KEVGNECPVCLSAF DGEEIRQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183
             CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPI+N APRRPAASRSDDFHQGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 184 GNLV 188
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Carg12559 vs. ExPASy TrEMBL
Match: A0A5D3DN65 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001760 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.9e-80
Identity = 146/184 (79.35%), Postives = 155/184 (84.24%), Query Frame = 0


Query: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63
           MDT+A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPAL YSF FAIKCPP  LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123
           R+RS RLS A VT+V GNR+  SDVA   KYNKDE  KEVG ECPVCLSAF DGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183
             CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPINN APRRPAASRSDDFHQGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 184 GNLV 188
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Carg12559 vs. ExPASy TrEMBL
Match: A0A1S3B6Q9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.9e-80
Identity = 146/184 (79.35%), Postives = 155/184 (84.24%), Query Frame = 0


Query: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63
           MDT+A+ MNRS PP+PTPHRT DFSPIEFILGL+AVI IPAL YSF FAIKCPP  LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123
           R+RS RLS A VT+V GNR+  SDVA   KYNKDE  KEVG ECPVCLSAF DGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183
             CKHSFHVDCIDMW YSHPNCPVCRA + + VKRPINN APRRPAASRSDDFHQGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 184 GNLV 188
           G LV
Sbjct: 181 GYLV 184

[Close]

BLAST of Carg12559 vs. TAIR 10
Match: AT2G37580.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 8.4e-33
Identity = 78/190 (41.05%), Postives = 107/190 (56.32%), Query Frame = 0


Query: 16  PPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLWRRRSARLS-GAP 75
           PP P P R+ D +P++ I  +IA +A+PALVY+ FF   C          R++  S   P
Sbjct: 48  PPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSSRRNSSSSRTSSSSDDTP 107

Query: 76  VTVVAGNRSSYSDVAVTF----KYNKDEVGKEVGNECPVCLSAFVDGEEIRQLIACKHSF 135
              V  +    ++  VT     K++KD   KE+GNEC VCL  F D +E+RQL  CKH+F
Sbjct: 108 HATV--DTPPITETTVTSESGGKFHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHAF 167

Query: 136 HVDCIDMWFYSHPNCPVCRAEIAI-----AVKRPIN-----NRA---PRRPAASRSDDFH 188
           HV CI+ W   HPNCP+CR ++++     A   P+N     NR+    R  A SR DD+ 
Sbjct: 168 HVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDWR 227

[Close]

BLAST of Carg12559 vs. TAIR 10
Match: AT4G15975.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.0e-14
Identity = 33/69 (47.83%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 106 ECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCRA--------------E 161
           EC VCLS F D E  R +  CKH+FHVDCIDMWF+SH +CP+CR+              E
Sbjct: 75  ECSVCLSEFKDNESGRVMPNCKHTFHVDCIDMWFHSHSSCPLCRSLIEPFAGGVKSTMDE 134

[Close]

BLAST of Carg12559 vs. TAIR 10
Match: AT1G72310.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 3.9e-14
Identity = 40/95 (42.11%), Postives = 52/95 (54.74%), Query Frame = 0


Query: 56  PPGLLRLWRRRSARLSGAPVTVVAGNRS-SYSDVAVTFKYNKDEVGKEVGNECPVCLSAF 115
           PP + R  RRR   + G       G  S   S + + F + +D    + G EC +CLS  
Sbjct: 78  PPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVF-FRQDSC--KDGLECSICLSEL 137

Query: 116 VDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCR 150
           V G++ R L  C HSFHV+CIDMWF SH  CP+CR
Sbjct: 138 VKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICR 169

[Close]

BLAST of Carg12559 vs. TAIR 10
Match: AT1G53820.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.7e-14
Identity = 30/46 (65.22%), Postives = 33/46 (71.74%), Query Frame = 0


Query: 104 GNECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCR 150
           G EC VCLS  VDG++ R L  C H FHVDCIDMWF SH  CP+CR
Sbjct: 117 GLECAVCLSDLVDGDKARVLPRCNHGFHVDCIDMWFQSHSTCPLCR 162

[Close]

BLAST of Carg12559 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.7e-14
Identity = 58/180 (32.22%), Postives = 80/180 (44.44%), Query Frame = 0


Query: 1   MSQMDTSAALMNRSTPPHPTPHRTNDFSPI---------EFILGLIAVIAIPA----LVY 60
           M   D      N   PP P P  T   SPI          F +  IAVI I A    LV 
Sbjct: 1   MDLTDRRNPFNNLVFPPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVS 60

Query: 61  SFFFAIKCPPG--LLRLWRRRSARLSGAPVTVVA------GNRSSYSDVAVTFKYNKDEV 120
            + F IKC      + ++RRR       P+ + +      G   S       FK+ K +V
Sbjct: 61  YYIFVIKCCLNWHQIDIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDV 120

Query: 121 --GKE----VGNECPVCLSAFVDGEEIRQLIACKHSFHVDCIDMWFYSHPNCPVCRAEIA 154
             G+E       EC VCL+ F + E++R +  C H FH+DCID+W   + NCP+CR  ++
Sbjct: 121 VAGEEDQSKNSQECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVS 180

The following BLAST results are available for this feature:

BLAST of Carg12559 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934546.1	2.2e-104	100.00	RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 fin...	[more]
XP_023528143.1	9.4e-103	98.93	RING-H2 finger protein ATL33-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983254.1	3.4e-100	95.72	RING-H2 finger protein ATL33-like [Cucurbita maxima]	[more]
XP_038905078.1	7.0e-82	82.61	RING-H2 finger protein ATL33 [Benincasa hispida]	[more]
KAE8651013.1	4.6e-81	79.57	hypothetical protein Csa_001104 [Cucumis sativus]	[more]

BLAST of Carg12559 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80927	1.2e-31	41.05	RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2	[more]
Q9XF63	5.5e-13	42.11	RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1	[more]
P93823	9.4e-13	32.22	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LX93	9.4e-13	34.86	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
P0C035	9.4e-13	65.22	RING-H2 finger protein ATL60 OS=Arabidopsis thaliana OX=3702 GN=ATL60 PE=2 SV=1	[more]

BLAST of Carg12559 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F238	1.1e-104	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690...	[more]
A0A6J1J6T6	1.6e-100	95.72	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 P...	[more]
A0A0A0LA67	4.9e-81	79.89	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE...	[more]
A0A5D3DN65	1.9e-80	79.35	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B6Q9	1.9e-80	79.35	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 ...	[more]

BLAST of Carg12559 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37580.1	8.4e-33	41.05	RING/U-box superfamily protein	[more]
AT4G15975.1	3.0e-14	47.83	RING/U-box superfamily protein	[more]
AT1G72310.1	3.9e-14	42.11	RING/U-box superfamily protein	[more]
AT1G53820.1	6.7e-14	65.22	RING/U-box superfamily protein	[more]
AT1G04360.1	6.7e-14	32.22	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 107..148 e-value: 5.5E-5 score: 32.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 105..149 e-value: 3.4E-13 score: 49.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 107..149 score: 12.208971
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 78..154 e-value: 5.6E-20 score: 72.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 164..187
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR45676:SF88	RING-H2 FINGER PROTEIN ATL33	coord: 11..187
None	No IPR available	CDD	cd16461	RING-H2_EL5_like	coord: 106..149 e-value: 5.5963E-17 score: 68.8759
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 106..155
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 11..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12559-RA	Carg12559-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane