Carg12464 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12464
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiondnaJ protein ERDJ3A
LocationCarg_Chr14: 219313 .. 222268 (-)
RNA-Seq ExpressionCarg12464
SyntenyCarg12464
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTCGTTCTGATCGGGGAAGAACCTATATTATTTCGCACCCGTCGCTTCGTCTCTCCCTCCATTTTACCATCTCCCAATTACCAAATTCCAGTTTACGAAATCATCAGTTGCAGAAACTCGGCTGGATCTGAACATGGAGATTCGTCTTTCGCCGTTCATTCTCATTATGGCGTCTTTGTTCCTTTTCCTGTTGTCGGAGGCCAAAACCATGGACCCTTACAAGGTAACGAAGATTGGTCGGAGTTTTTCTCTCATTGATACACTTTGCCATTTTTTTGTGTATCGTCATTTTATTATGTTGATGTTCGTCTTGATTTCCTCCTAATTGTTTATAACTTGAGAAATTTACATGCGTTTGGTTACGGAGTATGGAAGTTTGTGGTGGTGTTATAGAATGGCTCATCATCGTTGCGTTTTCTGAAATTGTTTTCAGGTTCTTGGAGTTGAGAGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGTATCTAACTTCCCTCCAAAATTTATGTTCTTGTTCCATTTGATTCAACTTCTTTTGGTTGTGTAGCTCATGGTGGAATTTATTAATCATTGGCAGGCTTTCTCTTCAGTATCATCCAGATAAGAATAAGAAAAAAAGCGCACAACATAAGTTTGAAGAAATCAATAACGGTATGTTACTTACTTCGATCGACCATTGTATCTTTTCGCCAGGGCTTTTCTACTGCAGTTAATCTCTTTTCCCTGTCATTTCGATTTGTGGGATGAGTTTTGGTTGAATAGGGTGTACTTAATTTGAGTCATGAAGTTCAACATTGGAGAAGAAAAATAATGTTGTCCTGAATTGATATCATGTTAAAGTACTACTAAACCAACATGTCTAAATCTGCCTAGTTTTCCACCACAATTGACCTTACTTCTCCATGTTTCAGCATATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGATTTGTATGGCGATGAAAAAGGTAGCCCTGGCTTTGGCCCTGGCTATTCAGGGGATCAAGGTGGTGGACATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGAACTTCAAGCCACAGTGGCAGGGTACTGGTGGACAAGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGACCGAGTGGTGGTTCAAATCCCTTCGGCTTTGGTATGGAGGATATATTTTCCAATTTTTTTGGAGGTGGCTTTAAGGATGAGGGTAATTTTGGTAGCCACTTTAAGGCCCAATCTGGATCTAAGAGTTCCTCAATCATCAAGGTCGTTAATAAACAAGTGTTTAAGCAAGAAGTTGTGGGCCAGGGAGTAACTTGGCTTCTGTTTCCCATCACGTCATCTCTGAAGGGAGTTGATCATGTTCAATCAATTATAGAGGATGTTGCCAGTTCGTTGCAGGGAGCTTTAAAGGTGCTATTAAGTAATCATATTTCTAAATTAAATAGTTATGGCGATTTTTCACTGTTCTCATCTTCTGTTTGACCTTTCAATTCAGGCTGGAAGGATAGATTGTGAATCAGAATCCTCTCTCTGCAAAGACCTTGGCATTTATCCTCATCGGACTCCTAGAATATTTGTCTATTCATACATAAAAAGCACCGATGGTTCTTTGGTAGAGTACAGTGGGGACGTTGCTGTGAAGAGTTTGAAAAGTTTTTGTCAAGAGCATCTTCCAAGGTTCTCACGACGGGTCGATTTGAGGCAATTTGACTTCTCTTCCAACAATGGAGGAAGATTACCCACTTTAATGCTTCTGTCAACCAAAAAGGATACACCTGTTATCTGGCGTGTCCTTAGCGGGATGTATAACAAGCAGTTCAGTTTCTATGATGCAGAGGTATATTATTACTGAGCTTGCTTGATTATCTCATTCTTGTATGAGGCTATCAGTATCCGTTGCATAATTATGTGGGTTATCTCTGTGGAGGTTCATCATTTTCATACTCTGATAGCAGATTTCTTTTTATTTTATTGCATAGGTCACGGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTCGATGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGAGAGAGGCACGTTCTCAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATTGATGATCTCAGTTATTTAGTTAATGGTTTTGAGAAAAAGAACAGGAAGGCAGCGTCAAAGCCAGCCTCCAAAACACAATCTGATTCTGCACAGAATCAGATCCCTCTATTGACAGGGTTCAACTTTGATTCTATCTGTGGGAAGAACACTCCTGTTTGTGTCATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAAATGATCCTAAACATGGTGAGAGCACTCGCATTTACGTTTTGTCTCAAGCCGCTGCACTTTGGCTTGTTGATTGCTGCTATGCTATACCTTACATTTTCTGCCCCTCTTGATTTAATGTTTCAGGTGTCTCAGAAGACATTGTCAAGGCGTCAGATCTCTGCGCCTGGCTCTAAGGAATCCATATCCTATTCTCTCGTAGATGCAACAAAACAACCATCGTTTCTTAAAGCATTTGACAGTGCTGGATTTAAATCATCTGATCAAATACTGATCGCATACAAACGTCGGAAGGAGAAATTTGCAGCACATTTGGGTGAAATGACGGAAGAAGAGGTAGAAAAATTTATCGGCTCAGTGCTTAATGGGGACATTACATTCACCAAGACCCGCCAGAAACCTGTCTTGAAGTGAAGGTCTGCCATTAACAAGTGTACTCAATTCTTGCGGTCTTATGTACATTTTATGAATATAGTTAGAATGAAGTAAAAGAAAAAAGAGTATATAGCTAAGGGAAGAAATTATTATCTGGAGAGAAATCTGTGTTGATGTCCTATTGAGGGTGCCTACATCAATGTTTGCCACCTGCATTTTGTGGGCAGAAAATCTTTTGTGTT

mRNA sequence

TATTTCGTTCTGATCGGGGAAGAACCTATATTATTTCGCACCCGTCGCTTCGTCTCTCCCTCCATTTTACCATCTCCCAATTACCAAATTCCAGTTTACGAAATCATCAGTTGCAGAAACTCGGCTGGATCTGAACATGGAGATTCGTCTTTCGCCGTTCATTCTCATTATGGCGTCTTTGTTCCTTTTCCTGTTGTCGGAGGCCAAAACCATGGACCCTTACAAGGTTCTTGGAGTTGAGAGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGCTTTCTCTTCAGTATCATCCAGATAAGAATAAGAAAAAAAGCGCACAACATAAGTTTGAAGAAATCAATAACGCATATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGATTTGTATGGCGATGAAAAAGGTAGCCCTGGCTTTGGCCCTGGCTATTCAGGGGATCAAGGTGGTGGACATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGAACTTCAAGCCACAGTGGCAGGGTACTGGTGGACAAGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGACCGAGTGGTGGTTCAAATCCCTTCGGCTTTGGTATGGAGGATATATTTTCCAATTTTTTTGGAGGTGGCTTTAAGGATGAGGGTAATTTTGGTAGCCACTTTAAGGCCCAATCTGGATCTAAGAGTTCCTCAATCATCAAGGTCGTTAATAAACAAGTGTTTAAGCAAGAAGTTGTGGGCCAGGGAGTAACTTGGCTTCTGTTTCCCATCACGTCATCTCTGAAGGGAGTTGATCATGTTCAATCAATTATAGAGGATGTTGCCAGTTCGTTGCAGGGAGCTTTAAAGGCTGGAAGGATAGATTGTGAATCAGAATCCTCTCTCTGCAAAGACCTTGGCATTTATCCTCATCGGACTCCTAGAATATTTGTCTATTCATACATAAAAAGCACCGATGGTTCTTTGGTAGAGTACAGTGGGGACGTTGCTGTGAAGAGTTTGAAAAGTTTTTGTCAAGAGCATCTTCCAAGGTTCTCACGACGGGTCGATTTGAGGCAATTTGACTTCTCTTCCAACAATGGAGGAAGATTACCCACTTTAATGCTTCTGTCAACCAAAAAGGATACACCTGTTATCTGGCGTGTCCTTAGCGGGATGTATAACAAGCAGTTCAGTTTCTATGATGCAGAGGTCACGGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTCGATGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGAGAGAGGCACGTTCTCAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATTGATGATCTCAGTTATTTAGTTAATGGTTTTGAGAAAAAGAACAGGAAGGCAGCGTCAAAGCCAGCCTCCAAAACACAATCTGATTCTGCACAGAATCAGATCCCTCTATTGACAGGGTTCAACTTTGATTCTATCTGTGGGAAGAACACTCCTGTTTGTGTCATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAAATGATCCTAAACATGGTGTCTCAGAAGACATTGTCAAGGCGTCAGATCTCTGCGCCTGGCTCTAAGGAATCCATATCCTATTCTCTCGTAGATGCAACAAAACAACCATCGTTTCTTAAAGCATTTGACAGTGCTGGATTTAAATCATCTGATCAAATACTGATCGCATACAAACGTCGGAAGGAGAAATTTGCAGCACATTTGGGTGAAATGACGGAAGAAGAGGTAGAAAAATTTATCGGCTCAGTGCTTAATGGGGACATTACATTCACCAAGACCCGCCAGAAACCTGTCTTGAAGTGAAGGTCTGCCATTAACAAGTGTACTCAATTCTTGCGGTCTTATGTACATTTTATGAATATAGTTAGAATGAAGTAAAAGAAAAAAGAGTATATAGCTAAGGGAAGAAATTATTATCTGGAGAGAAATCTGTGTTGATGTCCTATTGAGGGTGCCTACATCAATGTTTGCCACCTGCATTTTGTGGGCAGAAAATCTTTTGTGTT

Coding sequence (CDS)

ATGGAGATTCGTCTTTCGCCGTTCATTCTCATTATGGCGTCTTTGTTCCTTTTCCTGTTGTCGGAGGCCAAAACCATGGACCCTTACAAGGTTCTTGGAGTTGAGAGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGCTTTCTCTTCAGTATCATCCAGATAAGAATAAGAAAAAAAGCGCACAACATAAGTTTGAAGAAATCAATAACGCATATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGATTTGTATGGCGATGAAAAAGGTAGCCCTGGCTTTGGCCCTGGCTATTCAGGGGATCAAGGTGGTGGACATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGAACTTCAAGCCACAGTGGCAGGGTACTGGTGGACAAGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGACCGAGTGGTGGTTCAAATCCCTTCGGCTTTGGTATGGAGGATATATTTTCCAATTTTTTTGGAGGTGGCTTTAAGGATGAGGGTAATTTTGGTAGCCACTTTAAGGCCCAATCTGGATCTAAGAGTTCCTCAATCATCAAGGTCGTTAATAAACAAGTGTTTAAGCAAGAAGTTGTGGGCCAGGGAGTAACTTGGCTTCTGTTTCCCATCACGTCATCTCTGAAGGGAGTTGATCATGTTCAATCAATTATAGAGGATGTTGCCAGTTCGTTGCAGGGAGCTTTAAAGGCTGGAAGGATAGATTGTGAATCAGAATCCTCTCTCTGCAAAGACCTTGGCATTTATCCTCATCGGACTCCTAGAATATTTGTCTATTCATACATAAAAAGCACCGATGGTTCTTTGGTAGAGTACAGTGGGGACGTTGCTGTGAAGAGTTTGAAAAGTTTTTGTCAAGAGCATCTTCCAAGGTTCTCACGACGGGTCGATTTGAGGCAATTTGACTTCTCTTCCAACAATGGAGGAAGATTACCCACTTTAATGCTTCTGTCAACCAAAAAGGATACACCTGTTATCTGGCGTGTCCTTAGCGGGATGTATAACAAGCAGTTCAGTTTCTATGATGCAGAGGTCACGGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTCGATGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGAGAGAGGCACGTTCTCAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATTGATGATCTCAGTTATTTAGTTAATGGTTTTGAGAAAAAGAACAGGAAGGCAGCGTCAAAGCCAGCCTCCAAAACACAATCTGATTCTGCACAGAATCAGATCCCTCTATTGACAGGGTTCAACTTTGATTCTATCTGTGGGAAGAACACTCCTGTTTGTGTCATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAAATGATCCTAAACATGGTGTCTCAGAAGACATTGTCAAGGCGTCAGATCTCTGCGCCTGGCTCTAAGGAATCCATATCCTATTCTCTCGTAGATGCAACAAAACAACCATCGTTTCTTAAAGCATTTGACAGTGCTGGATTTAAATCATCTGATCAAATACTGATCGCATACAAACGTCGGAAGGAGAAATTTGCAGCACATTTGGGTGAAATGACGGAAGAAGAGGTAGAAAAATTTATCGGCTCAGTGCTTAATGGGGACATTACATTCACCAAGACCCGCCAGAAACCTGTCTTGAAGTGA

Protein sequence

MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Homology
BLAST of Carg12464 vs. NCBI nr
Match: XP_022935557.1 (dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017212.1 DnaJ protein ERDJ3A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK
Sbjct: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60

Query: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120
           KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR
Sbjct: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120

Query: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180
           QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS
Sbjct: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180

Query: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240
           HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ
Sbjct: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240

Query: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300
           GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ
Sbjct: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300

Query: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360
           EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT
Sbjct: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360

Query: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420
           DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA
Sbjct: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420

Query: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480
           ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK
Sbjct: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480

Query: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540
           TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG
Sbjct: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540

Query: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Sbjct: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Carg12464 vs. NCBI nr
Match: XP_023529005.1 (dnaJ protein ERDJ3A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 562/571 (98.42%), Postives = 567/571 (99.30%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK
Sbjct: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60

Query: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120
           KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGP YSGDQGGGHTFFTNEGRR
Sbjct: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPDYSGDQGGGHTFFTNEGRR 120

Query: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180
           QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS
Sbjct: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180

Query: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240
           HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ
Sbjct: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240

Query: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300
           GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVK+LKSFCQ
Sbjct: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKNLKSFCQ 300

Query: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360
           EH PRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT
Sbjct: 301 EHFPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360

Query: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420
           DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYL++GFEKKNRKA
Sbjct: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLLDGFEKKNRKA 420

Query: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480
           ASKPASKTQSDSAQN+IPLLTG NFDSICGKNTPVCVIGAF+SSKARNKLEMILNMVSQK
Sbjct: 421 ASKPASKTQSDSAQNRIPLLTGSNFDSICGKNTPVCVIGAFKSSKARNKLEMILNMVSQK 480

Query: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540
           TLSRRQISAP SKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG
Sbjct: 481 TLSRRQISAPSSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540

Query: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Sbjct: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Carg12464 vs. NCBI nr
Match: XP_022983581.1 (dnaJ protein ERDJ3A [Cucurbita maxima])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 559/571 (97.90%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           MEI LSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK
Sbjct: 1   MEICLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60

Query: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120
           KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR
Sbjct: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120

Query: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180
           Q QQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS
Sbjct: 121 QDQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180

Query: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240
           HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ
Sbjct: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240

Query: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300
           GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVK+L+SFCQ
Sbjct: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKNLRSFCQ 300

Query: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360
           EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQF FYDAEVT
Sbjct: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFRFYDAEVT 360

Query: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420
           DVSDPTIKKLGVDALPAIVGWLSNGERH+LRTGINVKDLRSAIDDLSYL+NGFEKKNRKA
Sbjct: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHILRTGINVKDLRSAIDDLSYLLNGFEKKNRKA 420

Query: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480
           ASKPASKTQSDSAQNQIPLLT  NFDSICGKNTPVCVIGAF+SSKARNKLEMILNMVSQK
Sbjct: 421 ASKPASKTQSDSAQNQIPLLTESNFDSICGKNTPVCVIGAFKSSKARNKLEMILNMVSQK 480

Query: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540
           TLSRRQISAPGSKESISYSLVDA KQPSFLKAFDSAGFK SDQILIAYKRRKEKFAAHLG
Sbjct: 481 TLSRRQISAPGSKESISYSLVDAAKQPSFLKAFDSAGFKPSDQILIAYKRRKEKFAAHLG 540

Query: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Sbjct: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Carg12464 vs. NCBI nr
Match: XP_038905464.1 (dnaJ protein ERDJ3A isoform X1 [Benincasa hispida])

HSP 1 Score: 969.5 bits (2505), Expect = 1.2e-278
Identity = 500/576 (86.81%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           MEIRL+   LIMA LFLFL S EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLASITLIMAFLFLFLCSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD+YG+EKGSPGFGPG S DQGGG+TFFTN   
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGNEKGSPGFGPGSSEDQGGGYTFFTNGAG 120

Query: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGN- 180
           RQGQQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFKDEGN 
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 --FGSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDV 240
             FGSH KAQSGS++SS IKV+NKQVFK+E+V QG+TWLLFP TSSLKGVDHVQSIIE+V
Sbjct: 181 GSFGSHSKAQSGSRTSSSIKVINKQVFKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEV 240

Query: 241 ASSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSL 300
           ASSLQGALK GRIDC+SESSLCKDLGIYPHRTPRIFVYSYIKST+GSLVEYSGDVAVK+L
Sbjct: 241 ASSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNL 300

Query: 301 KSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFY 360
           +SFCQEH PRFS+RV+L+QFDFSS++GGRLPTLMLLSTKKDTPVIWRVLSG++ KQFSFY
Sbjct: 301 RSFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVLSGLFRKQFSFY 360

Query: 361 DAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEK 420
           D EVTDVSDP++KKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLS L+NGFEK
Sbjct: 361 DTEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEK 420

Query: 421 KNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILN 480
           KNRKA S+PASKTQSDSA+NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE+ILN
Sbjct: 421 KNRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILN 480

Query: 481 MVSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKF 540
           MVSQKTLSRRQ S PGSKE+ISY+LVDA+KQPSFL AFD AGFKSSD+ILIAYKRRK KF
Sbjct: 481 MVSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAFDGAGFKSSDKILIAYKRRKGKF 540

Query: 541 AAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           AAHLGE+TEEEVEKFIGSVLNGD+TFTKTRQKP+LK
Sbjct: 541 AAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 575

BLAST of Carg12464 vs. NCBI nr
Match: TYK24944.1 (dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 942.6 bits (2435), Expect = 1.6e-270
Identity = 485/573 (84.64%), Postives = 525/573 (91.62%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M  RL+  IL++A LFLF LS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD+YGDEKG+PGFG G SGDQGGG+TFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180
           RQGQQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFKD+G+F
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASS 240
           G H KAQSG KSSS IKV+NKQ+ K+E+V QG+TWLLFP TSSLKGVDHVQSIIE+VAS+
Sbjct: 181 GGHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVAST 240

Query: 241 LQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSF 300
           LQGALK GRIDC+SESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VKSLK F
Sbjct: 241 LQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGF 300

Query: 301 CQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAE 360
           CQEH PRFS+RV+L+QFDFSS+N GRLPTLMLLSTKK+TPVIWRVLSG++ K F+FYDAE
Sbjct: 301 CQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAE 360

Query: 361 VTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNR 420
           VTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLS L+NGFEKKNR
Sbjct: 361 VTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNR 420

Query: 421 KAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVS 480
           KAAS+P SKTQSDS +NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE IL MVS
Sbjct: 421 KAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKMVS 480

Query: 481 QKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAH 540
           QKTLSRRQ S PGSKE+ISY+LVDATKQ SFL AFDSAGFKSSD+ILIAYKRRK KFAA+
Sbjct: 481 QKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKGKFAAY 540

Query: 541 LGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           LGE+TEEEVEKFIGSVLNGD+TFT TRQKP+LK
Sbjct: 541 LGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of Carg12464 vs. ExPASy Swiss-Prot
Match: Q9SR96 (DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 5.0e-182
Identity = 340/568 (59.86%), Postives = 424/568 (74.65%), Query Frame = 0

Query: 10  LIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFE 69
           +++ S  L L  +AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKNK K AQ KF 
Sbjct: 10  VVLVSTLLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKDKGAQEKFA 69

Query: 70  EINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQ 129
           EINNAYEILSDEEKRKN+DLYGDEKG PGF  G+ G  GG     +  G   G       
Sbjct: 70  EINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGGNGGYSYSSSGGGFNFGGPG---G 129

Query: 130 WQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDE---GNFGSHFKAQS 189
           WQ  GG GGS+SFSFSFGGPS  S  FGFGM+DIFS F GG  K +   G FGS   A+S
Sbjct: 130 WQNMGGGGGSKSFSFSFGGPSESS--FGFGMDDIFSMFSGGSSKGKEQFGGFGSSSNAES 189

Query: 190 GSKSSSI--IKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQGALK 249
            SKSS++  IK +N QV+K++VV QG+TWLL     S +G  + +SIIE+VA SLQGALK
Sbjct: 190 KSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVAESLQGALK 249

Query: 250 AGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQEHLP 309
            GR++CE+ESSLCK LGI P R PR+FVYSY  S   +L EY+ ++  K +KSFCQEHLP
Sbjct: 250 VGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVKSFCQEHLP 309

Query: 310 RFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVTDVSD 369
           RFS+++DL  FD S+ +  + P ++LLSTKKDTPVIWRVLSG+YN +F FY+ EV D SD
Sbjct: 310 RFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYNTEVHDTSD 369

Query: 370 PTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKAASKP 429
           P I+KLGVD  PAIVGWLSNGE+ VL+TGI VK+L+SA+ +L  L+ G EKKN+K +SK 
Sbjct: 370 PKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKKNKKVSSKS 429

Query: 430 ASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQKTLSR 489
            +    + +  +IPLL+  NFDSICG+NTPVC+IGAFRSS  + KL+ I++ VSQK+LSR
Sbjct: 430 QAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMSKVSQKSLSR 489

Query: 490 RQISAPGSKESISYSLVDATKQPSFLKAFDSAGFK-SSDQILIAYKRRKEKFAAHLGEMT 549
           RQ S  GS++++SYSL+DATKQ +FL + D + FK SSD++LIAYK R+ KFA   G+MT
Sbjct: 490 RQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAYKPRRGKFATFKGDMT 549

Query: 550 EEEVEKFIGSVLNGDITFTKTRQKPVLK 572
            EEVEKF+ +VLNGDI FTKTRQKP +K
Sbjct: 550 IEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of Carg12464 vs. ExPASy Swiss-Prot
Match: Q10MW6 (DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2)

HSP 1 Score: 555.1 bits (1429), Expect = 9.5e-157
Identity = 298/574 (51.92%), Postives = 401/574 (69.86%), Query Frame = 0

Query: 10  LIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFE 69
           ++++S+ L  ++ AK +DPYKVLGV+++ASQR+IQKAFHKLSL+YHPDKNK K AQ KF 
Sbjct: 13  IVLSSIALH-VAAAKNLDPYKVLGVDKSASQRDIQKAFHKLSLKYHPDKNKSKGAQEKFA 72

Query: 70  EINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQ 129
           EINNAY+ILSDEEKRKN+DLYGDEKG+PGFG G  G++  G+T+FT  G +    +    
Sbjct: 73  EINNAYDILSDEEKRKNYDLYGDEKGNPGFGGGNFGNR-EGYTYFTGGGAKTSHFSSGDG 132

Query: 130 WQGTGGQGGSQSFSFSFGG---PSGGSNPFGFGMEDIFSNFF-GGGFKDEGNFGSHFKAQ 189
           WQ  GGQG +++FSFSFGG    +GG NPF F   D+FSN F GG      + GS  KA+
Sbjct: 133 WQTMGGQGNTKTFSFSFGGGNPGAGGGNPFNFDFGDVFSNIFSGGSMGGSQHTGSAGKAR 192

Query: 190 SGSKSSS------IIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSL 249
            G+KSS        I+ V  Q+F +E   QG+TWLL   T + KG   ++S++EDVA SL
Sbjct: 193 RGTKSSGHDSSSVNIQEVTMQIFNKETADQGITWLLLFYTPNTKGQFVLESVVEDVARSL 252

Query: 250 QGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFC 309
            GAL+AG+++C+ E +LCK  G+   ++ R+F+YSY  +  GSL EYSGD   KSLK+FC
Sbjct: 253 DGALRAGKVNCDHEKALCKKAGVSIGKSARLFIYSYTTTEKGSLHEYSGDYDSKSLKTFC 312

Query: 310 QEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEV 369
           QEHLPRFS+RVD+ QF F SN    LP ++LLS KKDTP +WR +SGM+  +  FYDAEV
Sbjct: 313 QEHLPRFSKRVDINQFSFPSNIIPNLPQVLLLSAKKDTPAMWRAVSGMFRSRLIFYDAEV 372

Query: 370 TDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRK 429
            DVS P +K LGV  +PA++G   NGE  +L+ GI+VKDLRS I +L  L+  FEKKN+K
Sbjct: 373 QDVSHPLLKSLGVKNIPALIGRSVNGEEQLLKDGISVKDLRSGIKELKNLLENFEKKNKK 432

Query: 430 AASKPASKT--QSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMV 489
            AS  A K        +N+IPLLT  NF+ ICG+ T VC++G F+SSKA+  LE +L+ +
Sbjct: 433 LASNQAKKPAHTDQPKENKIPLLTASNFEEICGEKTSVCILGIFKSSKAKENLEAVLSEI 492

Query: 490 SQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAA 549
           SQKTL R Q    G+  +++Y+L+D  KQ +FL  FD + FKSSD++L+AYK R+ ++A 
Sbjct: 493 SQKTLIRGQNYNSGN--AVAYALLDGNKQSAFLSTFDKSAFKSSDKLLLAYKPRRGRYAV 552

Query: 550 HLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           +  E+T EE E+F+ SVLNGD+  +   +KPVL+
Sbjct: 553 YDNEVTMEEAERFVVSVLNGDVQLSAAGRKPVLR 582

BLAST of Carg12464 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 3.5e-18
Identity = 64/145 (44.14%), Postives = 84/145 (57.93%), Query Frame = 0

Query: 27  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKN 86
           D Y+VLGV ++A+  +I+ AF K ++QYHPD+NK+  A+ KF+E+N AYE+LSD +KR N
Sbjct: 6   DYYEVLGVSKDATDDQIKSAFRKKAMQYHPDRNKEPDAEEKFKEVNQAYEVLSDPDKRAN 65

Query: 87  FDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQGGSQSFSFSF 146
           +D +G E        G  G  GGG  F           +   Q+ G GG+GG   F  SF
Sbjct: 66  YDRFGHE--------GVDGQFGGGAGF--------DPFDIFNQFFG-GGRGGGAHFEQSF 124

Query: 147 GGPSGGSNPFGFGMEDIFSNFFGGG 172
           GG          G EDIFSNFFGGG
Sbjct: 126 GGS---------GFEDIFSNFFGGG 124

BLAST of Carg12464 vs. ExPASy Swiss-Prot
Match: P35514 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 3.0e-17
Identity = 63/143 (44.06%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 29  YKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKNFD 88
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++ A+ K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEEKYKEVQEAYETLSDEQKRAAYD 66

Query: 89  LYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQGGSQSFSFSFGG 148
            YG+   + GF                                G GG GG+  FS  FGG
Sbjct: 67  QYGEAGANGGF--------------------------------GGGGFGGASGFS-GFGG 112

Query: 149 PSGGSNPFGFGMEDIFSNFFGGG 172
            SGG   FG G EDIFS+FFGGG
Sbjct: 127 GSGG---FG-GFEDIFSSFFGGG 112

BLAST of Carg12464 vs. ExPASy Swiss-Prot
Match: A2RP20 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.0e-17
Identity = 63/143 (44.06%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 29  YKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKNFD 88
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++ A+ K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYD 66

Query: 89  LYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQGGSQSFSFSFGG 148
            YG+   + GF                                G GG GG+  FS  FGG
Sbjct: 67  QYGEAGANGGF--------------------------------GGGGFGGASGFS-GFGG 112

Query: 149 PSGGSNPFGFGMEDIFSNFFGGG 172
            SGG   FG G EDIFS+FFGGG
Sbjct: 127 ASGG---FG-GFEDIFSSFFGGG 112

BLAST of Carg12464 vs. ExPASy TrEMBL
Match: A0A6J1F5W9 (dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1)

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK
Sbjct: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60

Query: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120
           KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR
Sbjct: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120

Query: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180
           QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS
Sbjct: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180

Query: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240
           HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ
Sbjct: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240

Query: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300
           GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ
Sbjct: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300

Query: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360
           EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT
Sbjct: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360

Query: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420
           DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA
Sbjct: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420

Query: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480
           ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK
Sbjct: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480

Query: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540
           TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG
Sbjct: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540

Query: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Sbjct: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Carg12464 vs. ExPASy TrEMBL
Match: A0A6J1J2Q7 (dnaJ protein ERDJ3A OS=Cucurbita maxima OX=3661 GN=LOC111482142 PE=4 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 559/571 (97.90%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           MEI LSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK
Sbjct: 1   MEICLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60

Query: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120
           KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR
Sbjct: 61  KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRR 120

Query: 121 QGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180
           Q QQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS
Sbjct: 121 QDQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNFGS 180

Query: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240
           HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ
Sbjct: 181 HFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQ 240

Query: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQ 300
           GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVK+L+SFCQ
Sbjct: 241 GALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKNLRSFCQ 300

Query: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVT 360
           EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQF FYDAEVT
Sbjct: 301 EHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFRFYDAEVT 360

Query: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKA 420
           DVSDPTIKKLGVDALPAIVGWLSNGERH+LRTGINVKDLRSAIDDLSYL+NGFEKKNRKA
Sbjct: 361 DVSDPTIKKLGVDALPAIVGWLSNGERHILRTGINVKDLRSAIDDLSYLLNGFEKKNRKA 420

Query: 421 ASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQK 480
           ASKPASKTQSDSAQNQIPLLT  NFDSICGKNTPVCVIGAF+SSKARNKLEMILNMVSQK
Sbjct: 421 ASKPASKTQSDSAQNQIPLLTESNFDSICGKNTPVCVIGAFKSSKARNKLEMILNMVSQK 480

Query: 481 TLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAHLG 540
           TLSRRQISAPGSKESISYSLVDA KQPSFLKAFDSAGFK SDQILIAYKRRKEKFAAHLG
Sbjct: 481 TLSRRQISAPGSKESISYSLVDAAKQPSFLKAFDSAGFKPSDQILIAYKRRKEKFAAHLG 540

Query: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK
Sbjct: 541 EMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Carg12464 vs. ExPASy TrEMBL
Match: A0A5D3DMX5 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00600 PE=4 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.8e-271
Identity = 485/573 (84.64%), Postives = 525/573 (91.62%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M  RL+  IL++A LFLF LS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD+YGDEKG+PGFG G SGDQGGG+TFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180
           RQGQQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFKD+G+F
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASS 240
           G H KAQSG KSSS IKV+NKQ+ K+E+V QG+TWLLFP TSSLKGVDHVQSIIE+VAS+
Sbjct: 181 GGHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVAST 240

Query: 241 LQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSF 300
           LQGALK GRIDC+SESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VKSLK F
Sbjct: 241 LQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGF 300

Query: 301 CQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAE 360
           CQEH PRFS+RV+L+QFDFSS+N GRLPTLMLLSTKK+TPVIWRVLSG++ K F+FYDAE
Sbjct: 301 CQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAE 360

Query: 361 VTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNR 420
           VTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLS L+NGFEKKNR
Sbjct: 361 VTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNR 420

Query: 421 KAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVS 480
           KAAS+P SKTQSDS +NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE IL MVS
Sbjct: 421 KAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKMVS 480

Query: 481 QKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFAAH 540
           QKTLSRRQ S PGSKE+ISY+LVDATKQ SFL AFDSAGFKSSD+ILIAYKRRK KFAA+
Sbjct: 481 QKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKGKFAAY 540

Query: 541 LGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           LGE+TEEEVEKFIGSVLNGD+TFT TRQKP+LK
Sbjct: 541 LGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of Carg12464 vs. ExPASy TrEMBL
Match: A0A5A7TMT4 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005460 PE=4 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 1.9e-269
Identity = 485/574 (84.49%), Postives = 525/574 (91.46%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M  RL+  IL++A LFLF LS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD+YGDEKG+PGFG G SGDQGGG+TFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180
           RQGQQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFKD+G+F
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASS 240
           G H KAQSG KSSS IKV+NKQ+ K+E+V QG+TWLLFP TSSLKGVDHVQSIIE+VAS+
Sbjct: 181 GGHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVAST 240

Query: 241 LQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSF 300
           LQGALK GRIDC+SESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VKSLK F
Sbjct: 241 LQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGF 300

Query: 301 CQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAE 360
           CQEH PRFS+RV+L+QFDFSS+N GRLPTLMLLSTKK+TPVIWRVLSG++ K F+FYDAE
Sbjct: 301 CQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAE 360

Query: 361 VTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNR 420
           VTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLS L+NGFEKKNR
Sbjct: 361 VTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNR 420

Query: 421 KAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVS 480
           KAAS+P SKTQSDS +NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE IL MVS
Sbjct: 421 KAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKMVS 480

Query: 481 QKTLSRRQISAPGSKESISYSLVDATKQPSFLKAF-DSAGFKSSDQILIAYKRRKEKFAA 540
           QKTLSRRQ S PGSKE+ISY+LVDATKQ SFL AF DSAGFKSSD+ILIAYKRRK KFAA
Sbjct: 481 QKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRKGKFAA 540

Query: 541 HLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           +LGE+TEEEVEKFIGSVLNGD+TFT TRQKP+LK
Sbjct: 541 YLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of Carg12464 vs. ExPASy TrEMBL
Match: A0A1S3B542 (dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 1.9e-269
Identity = 485/574 (84.49%), Postives = 525/574 (91.46%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M  RL+  IL++A LFLF LS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD+YGDEKG+PGFG G SGDQGGG+TFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180
           RQGQQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFKD+G+F
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASS 240
           G H KAQSG KSSS IKV+NKQ+ K+E+V QG+TWLLFP TSSLKGVDHVQSIIE+VAS+
Sbjct: 181 GGHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVAST 240

Query: 241 LQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSF 300
           LQGALK GRIDC+SESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VKSLK F
Sbjct: 241 LQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGF 300

Query: 301 CQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAE 360
           CQEH PRFS+RV+L+QFDFSS+N GRLPTLMLLSTKK+TPVIWRVLSG++ K F+FYDAE
Sbjct: 301 CQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAE 360

Query: 361 VTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNR 420
           VTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLS L+NGFEKKNR
Sbjct: 361 VTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNR 420

Query: 421 KAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVS 480
           KAAS+P SKTQSDS +NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE IL MVS
Sbjct: 421 KAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKMVS 480

Query: 481 QKTLSRRQISAPGSKESISYSLVDATKQPSFLKAF-DSAGFKSSDQILIAYKRRKEKFAA 540
           QKTLSRRQ S PGSKE+ISY+LVDATKQ SFL AF DSAGFKSSD+ILIAYKRRK KFAA
Sbjct: 481 QKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRKGKFAA 540

Query: 541 HLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 572
           +LGE+TEEEVEKFIGSVLNGD+TFT TRQKP+LK
Sbjct: 541 YLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of Carg12464 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 639.0 bits (1647), Expect = 3.5e-183
Identity = 340/568 (59.86%), Postives = 424/568 (74.65%), Query Frame = 0

Query: 10  LIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFE 69
           +++ S  L L  +AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKNK K AQ KF 
Sbjct: 10  VVLVSTLLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKDKGAQEKFA 69

Query: 70  EINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQ 129
           EINNAYEILSDEEKRKN+DLYGDEKG PGF  G+ G  GG     +  G   G       
Sbjct: 70  EINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGGNGGYSYSSSGGGFNFGGPG---G 129

Query: 130 WQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDE---GNFGSHFKAQS 189
           WQ  GG GGS+SFSFSFGGPS  S  FGFGM+DIFS F GG  K +   G FGS   A+S
Sbjct: 130 WQNMGGGGGSKSFSFSFGGPSESS--FGFGMDDIFSMFSGGSSKGKEQFGGFGSSSNAES 189

Query: 190 GSKSSSI--IKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASSLQGALK 249
            SKSS++  IK +N QV+K++VV QG+TWLL     S +G  + +SIIE+VA SLQGALK
Sbjct: 190 KSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVAESLQGALK 249

Query: 250 AGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSFCQEHLP 309
            GR++CE+ESSLCK LGI P R PR+FVYSY  S   +L EY+ ++  K +KSFCQEHLP
Sbjct: 250 VGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVKSFCQEHLP 309

Query: 310 RFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAEVTDVSD 369
           RFS+++DL  FD S+ +  + P ++LLSTKKDTPVIWRVLSG+YN +F FY+ EV D SD
Sbjct: 310 RFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYNTEVHDTSD 369

Query: 370 PTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNRKAASKP 429
           P I+KLGVD  PAIVGWLSNGE+ VL+TGI VK+L+SA+ +L  L+ G EKKN+K +SK 
Sbjct: 370 PKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKKNKKVSSKS 429

Query: 430 ASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNMVSQKTLSR 489
            +    + +  +IPLL+  NFDSICG+NTPVC+IGAFRSS  + KL+ I++ VSQK+LSR
Sbjct: 430 QAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMSKVSQKSLSR 489

Query: 490 RQISAPGSKESISYSLVDATKQPSFLKAFDSAGFK-SSDQILIAYKRRKEKFAAHLGEMT 549
           RQ S  GS++++SYSL+DATKQ +FL + D + FK SSD++LIAYK R+ KFA   G+MT
Sbjct: 490 RQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAYKPRRGKFATFKGDMT 549

Query: 550 EEEVEKFIGSVLNGDITFTKTRQKPVLK 572
            EEVEKF+ +VLNGDI FTKTRQKP +K
Sbjct: 550 IEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of Carg12464 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 83.2 bits (204), Expect = 7.5e-16
Identity = 42/78 (53.85%), Postives = 57/78 (73.08%), Query Frame = 0

Query: 27  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKN 86
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK   A+ KF+EI+NAYE+LSD+EK+  
Sbjct: 86  DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEKKSL 145

Query: 87  FDLYGDE--KGSPGFGPG 103
           +D YG+   KG+ GFG G
Sbjct: 146 YDRYGEAGLKGAAGFGNG 163

BLAST of Carg12464 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 80.1 bits (196), Expect = 6.4e-15
Identity = 51/112 (45.54%), Postives = 65/112 (58.04%), Query Frame = 0

Query: 27  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKSAQHKFEEINNAYEILSDEEKRKN 86
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK   A+ KF+EI+NAYEILSD+EKR  
Sbjct: 85  DFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAEDKFKEISNAYEILSDDEKRSL 144

Query: 87  FDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQGG 139
           +D YG E G  G G G  GD                   F+  ++G GG GG
Sbjct: 145 YDRYG-EAGVKGAGMGGMGDYSNPFDL------------FESLFEGMGGMGG 183

BLAST of Carg12464 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 75.1 bits (183), Expect = 2.0e-13
Identity = 63/176 (35.80%), Postives = 86/176 (48.86%), Query Frame = 0

Query: 1   MEIRLSPFILIMASLFLFLLSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNK 60
           M IR S   +++ +L  + +        Y VL V + AS  +I++A+ KL+L+YHPDKN+
Sbjct: 1   MAIRWSELCIVLFAL-SYAICVLAGKSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQ 60

Query: 61  -KKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120
             + A  KF EINNAYE+LSDEEKR+ ++ YG+E               G   F  N GR
Sbjct: 61  GNEEATRKFAEINNAYEVLSDEEKREIYNKYGEE---------------GLKQFSANGGR 120

Query: 121 RQGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDE 176
                         GG GG                  G  M+DIFS+FFGGG  +E
Sbjct: 121 --------------GGGGG------------------GMNMQDIFSSFFGGGSMEE 128

BLAST of Carg12464 vs. TAIR 10
Match: AT5G03160.1 (homolog of mamallian P58IPK )

HSP 1 Score: 74.7 bits (182), Expect = 2.7e-13
Identity = 58/142 (40.85%), Postives = 74/142 (52.11%), Query Frame = 0

Query: 20  LSEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN--KKKSAQHKFEEINNAYEI 79
           L  +K  D YK+LG+ R AS  EI+KA+ KL+LQ+HPDKN   ++ A++KF EI  AYEI
Sbjct: 363 LKMSKRKDWYKILGISRTASISEIKKAYKKLALQWHPDKNVGNREEAENKFREIAAAYEI 422

Query: 80  LSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGRRQGQQNFKPQWQGTGGQG 139
           L D++KR  FD   D +   G G       GGG+  F                 G GG G
Sbjct: 423 LGDDDKRARFDRGEDLEDMGGGG-------GGGYNPF----------------HGGGGGG 481

Query: 140 GSQSFSFSFGGPSGGSNPFGFG 160
              +F F  G P GG    GFG
Sbjct: 483 QQYTFHFEGGFPGGGGGFGGFG 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022935557.10.0e+00100.00dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, part... [more]
XP_023529005.10.0e+0098.42dnaJ protein ERDJ3A [Cucurbita pepo subsp. pepo][more]
XP_022983581.10.0e+0097.90dnaJ protein ERDJ3A [Cucurbita maxima][more]
XP_038905464.11.2e-27886.81dnaJ protein ERDJ3A isoform X1 [Benincasa hispida][more]
TYK24944.11.6e-27084.64dnaJ protein ERDJ3A [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SR965.0e-18259.86DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1[more]
Q10MW69.5e-15751.92DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2[more]
Q8EUM43.5e-1844.14Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ P... [more]
P355143.0e-1744.06Chaperone protein DnaJ OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=27... [more]
A2RP203.0e-1744.06Chaperone protein DnaJ OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1F5W90.0e+00100.00dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1[more]
A0A6J1J2Q70.0e+0097.90dnaJ protein ERDJ3A OS=Cucurbita maxima OX=3661 GN=LOC111482142 PE=4 SV=1[more]
A0A5D3DMX57.8e-27184.64DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G... [more]
A0A5A7TMT41.9e-26984.49DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G... [more]
A0A1S3B5421.9e-26984.49dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G08970.13.5e-18359.86DNAJ heat shock N-terminal domain-containing protein [more]
AT2G22360.17.5e-1653.85DNAJ heat shock family protein [more]
AT4G39960.16.4e-1545.54Molecular chaperone Hsp40/DnaJ family protein [more]
AT3G62600.12.0e-1335.80DNAJ heat shock family protein [more]
AT5G03160.12.7e-1340.85homolog of mamallian P58IPK [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 61..81
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 194..304
e-value: 1.1E-7
score: 34.0
NoneNo IPR availablePANTHERPTHR45184DNAJ PROTEIN ERDJ3Acoord: 6..571
NoneNo IPR availablePANTHERPTHR45184:SF1DNAJ PROTEIN ERDJ3Acoord: 6..571
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 47..62
score: 45.07
coord: 83..102
score: 31.21
coord: 29..47
score: 50.49
coord: 63..83
score: 50.53
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 26..83
e-value: 1.1E-29
score: 114.6
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 27..88
e-value: 8.7E-26
score: 89.8
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 27..91
score: 21.954786
IPR001623DnaJ domainCDDcd06257DnaJcoord: 27..80
e-value: 3.13141E-24
score: 93.7641
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 24..180
e-value: 2.6E-28
score: 100.7
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 15..94
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 68..87
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 191..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12464-RACarg12464-RAmRNA