Carg12428 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12428
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nudC domain-containing protein 2-like
Location	Carg_Chr01: 3542068 .. 3544388 (+)
RNA-Seq Expression	Carg12428
Synteny	Carg12428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAACTTCTCGTTAGAGAGAAACAAACCAAGGGAAAAGCTTCTGTGTGAAACTCGCGGGAAAGGGGAAGAAGGAAGAAGCGAACCCTAAGAAATGGCAGAGAAATTGGCTCCAGAAAAGCGCCATAGCTTTCTCCACAACGGTCTTTTCTATCTCTTCATCAATTTCATCTTCGGAATTTTCAATTTGCCGAATTAATTCACTGTATTACTAGTTTTTTTTTTTTTTTTTCTTTTCAGGTCAGGAAGTGTTTGAATGGGATCAGACTCTGGAGGAGGTTAATATGTATATTAATTTGCCTCCAAATGTTCATTCAAAGCAATTCTACTGCAAGATTCAGTCTAGACATGTCGAGCTTGGGATTAAGGGGAATCCTCCTTATCTCAATGTCAGTATCATTGCATTTTCACCTCTCTTTCTTGCTTGATTTAAGTTTGATGTATTAATTCGTATGTTGTATTGATTTCTTTGCGTTGATTTGCAGCACGAACTTACTTGCCCTGTGAAGACGGATTCGTCTTTCTGGACGCTAGGTGCTTGCGAATTCTCGCGTTAATGATTAACTATAATTTTGAGGCGGTTGAATGTTATGATTTTAGACCAAATATATGGAATTTCTAATCAGATTATTGATGTCTCTTTTTGTATTTCCCTTCTATTGCTCCGATTTTGTCTATTTTGTGGTGTAATTGTTTTGTTTATTTGGTGTGGCAGAGGATGGCATAATGCACATTACACTGCAGAAGAGGGACAAGGGTCAGACATGGGCTTCTCCCATACAGGGTCCGGGTCAACTTGATCCTTACTCCACTGATCTTGAGCAGAAGCGTCTTATGCTCCAAAGGTTTCAGGAGGAGGTCCGTTTCTTTGTTCCATCTTTTTAGATGATATGAATACGTTTTGTGCTTTTCATGTTTGTTTTATGTTCCTTTTGTTTCTTTTCTTGAGCATTTGTTCATTTTTAGTCAGTGATCAATTGCTTCAAGCTGTCAAGATAGAAAAAAGAGAGAGAGAAAAAAAAAAAAAAAATCTCTAGATGCATACATGAAATCTTTAACTAATGTAGAGTACATGAACCATTTCTTCCAAATTTTCAATTCATAAGAGACGGCCTGGTTTTTCTCATACATCACCGAGGACCAAGCAAGAGAAAATACAATGATAGTCTATTTTGTTTGATTTCTTCTCTTTGAAGATATCCCTTAAGTAGTCAATTAGGTGCAGCAACAAGAGGGTGAAGAAACTCCTAACCCAAAAGCTTCACTCATTTTCCTTCAAGGCACTTCCAAGATGACTTGAACATATGATGAGCTCTTGTACTAACTGATGTTGGATAGATGAACCAATTTTGCAAAGCTGCAGATGATAGGACTTGTGATATAACCCAAATATTCCTACACTTCATCCATGACCAAGCTCAAGAATTCATCTGCAAGGCTACTCCATTTGGAGCCTATCCTTGGTGGCAACCTTTAGAACCCAATCGAGTTGAAGGAAAATATCTCATAAGTCCATTCTCAAGTTCATGGGATGCCCTGCATTACTTGTTTTGTTGTATTTTGAATTCCATATTTTTGTATTTTTAAGCTTTTGATTTTTTTTTTTTTTGGATGGTATAGATATAATTGGGGTTGCATTCAATCATTGCATACGAATGTTATCCACTCTGCATGTCATACAGACACACATATGGATGGAGGAGTTGTAGTATTCTCGTTAGTTTACTAAAATTTTATTGGGTGGAATGCTTTTGAAAAGTTTTGATACCTTTGAAATTCATGAACTTGTTTCATTTCCTACATAATTTCATATGTCCAGAGTCGTGTCAGTCATGTAATGAAATCTTGAAATTTAATATGCTCTCATAGTTCCTTTGCAGAACCCTGGATTTGACTTCTCACAAGCTCAGTTTTCGGGCAACTGTCCCGATCCAAGGACCTTCATGGGTGGAATTCACTCAGATTGACAACTATAGTTCGTGGCATTTATAACTTGCATGGGCATGACACTCTATTAATCCTCAGTAAATAATGTAGGTTTTCTCCTGGTCTTGATTTATGCAATCCCTGTAATAGCTGCTATTGTGGAAGGGAAATTTACCGAGTCTGCGCGCAATAGCTGCAATGAATTAAAAAGATTTAGTTGATATATATTTATCTACCAAATACTTTGTATTATAAGCTTTTAACCTTCTATTTGGTTTGATATAAACAAAACTATGTCCTAGAGGTCTATAGCAGATGGAAGTACATGGTAAACGTAGCAACTGATATCGTCATTATCTATATATTAGTTTGCCCATATCCCAATAAATTATGTTGA

mRNA sequence

GGAACTTCTCGTTAGAGAGAAACAAACCAAGGGAAAAGCTTCTGTGTGAAACTCGCGGGAAAGGGGAAGAAGGAAGAAGCGAACCCTAAGAAATGGCAGAGAAATTGGCTCCAGAAAAGCGCCATAGCTTTCTCCACAACGGTCAGGAAGTGTTTGAATGGGATCAGACTCTGGAGGAGGTTAATATGTATATTAATTTGCCTCCAAATGTTCATTCAAAGCAATTCTACTGCAAGATTCAGTCTAGACATGTCGAGCTTGGGATTAAGGGGAATCCTCCTTATCTCAATCACGAACTTACTTGCCCTGTGAAGACGGATTCGTCTTTCTGGACGCTAGAGGATGGCATAATGCACATTACACTGCAGAAGAGGGACAAGGGTCAGACATGGGCTTCTCCCATACAGGGTCCGGGTCAACTTGATCCTTACTCCACTGATCTTGAGCAGAAGCGTCTTATGCTCCAAAGGTTTCAGGAGGAGAACCCTGGATTTGACTTCTCACAAGCTCAGTTTTCGGGCAACTGTCCCGATCCAAGGACCTTCATGGGTGGAATTCACTCAGATTGACAACTATAGTTCGTGGCATTTATAACTTGCATGGGCATGACACTCTATTAATCCTCAGTAAATAATGTAGGTTTTCTCCTGGTCTTGATTTATGCAATCCCTGTAATAGCTGCTATTGTGGAAGGGAAATTTACCGAGTCTGCGCGCAATAGCTGCAATGAATTAAAAAGATTTAGTTGATATATATTTATCTACCAAATACTTTGTATTATAAGCTTTTAACCTTCTATTTGGTTTGATATAAACAAAACTATGTCCTAGAGGTCTATAGCAGATGGAAGTACATGGTAAACGTAGCAACTGATATCGTCATTATCTATATATTAGTTTGCCCATATCCCAATAAATTATGTTGA

Coding sequence (CDS)

ATGGCAGAGAAATTGGCTCCAGAAAAGCGCCATAGCTTTCTCCACAACGGTCAGGAAGTGTTTGAATGGGATCAGACTCTGGAGGAGGTTAATATGTATATTAATTTGCCTCCAAATGTTCATTCAAAGCAATTCTACTGCAAGATTCAGTCTAGACATGTCGAGCTTGGGATTAAGGGGAATCCTCCTTATCTCAATCACGAACTTACTTGCCCTGTGAAGACGGATTCGTCTTTCTGGACGCTAGAGGATGGCATAATGCACATTACACTGCAGAAGAGGGACAAGGGTCAGACATGGGCTTCTCCCATACAGGGTCCGGGTCAACTTGATCCTTACTCCACTGATCTTGAGCAGAAGCGTCTTATGCTCCAAAGGTTTCAGGAGGAGAACCCTGGATTTGACTTCTCACAAGCTCAGTTTTCGGGCAACTGTCCCGATCCAAGGACCTTCATGGGTGGAATTCACTCAGATTGA

Protein sequence

MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQKRLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD

Homology

[Close]

BLAST of Carg12428 vs. NCBI nr
Match: KAG7036985.1 (NudC domain-containing protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 344.4 bits (882), Expect = 5.4e-91
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. NCBI nr
Match: KAG6607306.1 (NudC domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 341.3 bits (874), Expect = 4.5e-90
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG GQLDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. NCBI nr
Match: XP_022948776.1 (nudC domain-containing protein 2-like [Cucurbita moschata])

HSP 1 Score: 339.7 bits (870), Expect = 1.3e-89
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG G+LDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGRLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. NCBI nr
Match: XP_023523271.1 (nudC domain-containing protein 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.8 bits (865), Expect = 5.0e-89
Identity = 155/158 (98.10%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVN+YINLPPNVHSKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNIYINLPPNVHSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYL+HELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG GQLDPYSTDLEQK
Sbjct: 61  NPPYLDHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. NCBI nr
Match: XP_022998001.1 (nudC domain-containing protein 2-like [Cucurbita maxima])

HSP 1 Score: 332.8 bits (852), Expect = 1.6e-87
Identity = 152/158 (96.20%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSF+HNGQ+VFEWDQTLEEVN+YINLPPNVHSKQFYCKIQSRHVELGIK 
Sbjct: 1   MAEKLAPEKRHSFIHNGQKVFEWDQTLEEVNIYINLPPNVHSKQFYCKIQSRHVELGIKR 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG GQLDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFS+AQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSKAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. ExPASy Swiss-Prot
Match: Q8WVJ2 (NudC domain-containing protein 2 OS=Homo sapiens OX=9606 GN=NUDCD2 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.7e-21
Identity = 56/140 (40.00%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 8   EKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNH 67
           E+R   +  G    +W QTLEEV + + +PP   ++   C +QSRHV L + G    L  
Sbjct: 6   EERSGVVPCGTPWGQWYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALSV-GGREILKG 65

Query: 68  ELTCPVKTDSSFWTLEDGIM---HITLQKRDKGQTWASPIQGPGQLDPYSTDLEQKRLML 127
           +L      D   WTLED  M    +T  KRD    W S ++     DP+  D  Q++L L
Sbjct: 66  KLFDSTIADEGTWTLEDRKMVRIVLTKTKRDAANCWTSLLESEYAADPWVQDQMQRKLTL 125

Query: 128 QRFQEENPGFDFSQAQFSGN 145
           +RFQ+ENPGFDFS A+ SGN
Sbjct: 126 ERFQKENPGFDFSGAEISGN 144

[Close]

BLAST of Carg12428 vs. ExPASy Swiss-Prot
Match: Q9CQ48 (NudC domain-containing protein 2 OS=Mus musculus OX=10090 GN=Nudcd2 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.7e-21
Identity = 56/140 (40.00%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 8   EKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNH 67
           E+R   +  G    +W QTLEEV + + +PP   ++   C +QSRHV L + G    L  
Sbjct: 6   EERSGVVPCGTPWGQWYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALAV-GGREILKG 65

Query: 68  ELTCPVKTDSSFWTLEDGIM---HITLQKRDKGQTWASPIQGPGQLDPYSTDLEQKRLML 127
           +L      D   WTLED  M    +T  KRD    W S ++     DP+  D  Q++L L
Sbjct: 66  KLFDSTIADEGTWTLEDRKMVRIVLTKTKRDAANCWTSLLESEYAADPWVQDQMQRKLTL 125

Query: 128 QRFQEENPGFDFSQAQFSGN 145
           +RFQ+ENPGFDFS A+ SGN
Sbjct: 126 ERFQKENPGFDFSGAEISGN 144

[Close]

BLAST of Carg12428 vs. ExPASy Swiss-Prot
Match: Q5M823 (NudC domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Nudcd2 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.9e-21
Identity = 53/126 (42.06%), Postives = 71/126 (56.35%), Query Frame = 0


Query: 22  EWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSSFWT 81
           +W QTLEEV + + +PP   ++   C +QSRHV L + G    L  +L      D   WT
Sbjct: 20  QWYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALAV-GGREILKGKLFDSTIADEGTWT 79

Query: 82  LEDGIM---HITLQKRDKGQTWASPIQGPGQLDPYSTDLEQKRLMLQRFQEENPGFDFSQ 141
           LED  M    +T  KRD    W S ++     DP+  D  Q++L L+RFQ+ENPGFDFS 
Sbjct: 80  LEDRKMVRIVLTKTKRDAANCWTSLLESEYAADPWVQDQMQRKLTLERFQKENPGFDFSG 139

Query: 142 AQFSGN 145
           A+ SGN
Sbjct: 140 AEISGN 144

[Close]

BLAST of Carg12428 vs. ExPASy Swiss-Prot
Match: Q9STN7 (Protein BOBBER 2 OS=Arabidopsis thaliana OX=3702 GN=BOB2 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.0e-13
Identity = 44/160 (27.50%), Postives = 75/160 (46.88%), Query Frame = 0


Query: 19  EVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSS 78
           E + W Q L+EV + I +P    S+   C+I+   +++G+KG    ++ E    VK D  
Sbjct: 134 EKYSWGQNLQEVTINIPMPEGTKSRSVTCEIKKNRLKVGLKGQDLIVDGEFFNSVKPDDC 193

Query: 79  FWTLED-GIMHITLQKRDKGQTWASPIQGP---------------GQLDPYS-------- 138
           FW +ED  ++ + L K+D+ + W   ++G                G LDP +        
Sbjct: 194 FWNIEDQKMISVLLTKQDQMEWWKYCVKGEPEIDTQKVEPETSKLGDLDPETRASVEKMM 253

Query: 139 ------------TDLEQKRLMLQRFQEENPGFDFSQAQFS 143
                       +D  +K+ ML++F  +NPG DFS A+F+
Sbjct: 254 FDQRQKQMGLPRSDEIEKKDMLKKFMAQNPGMDFSNAKFN 293

[Close]

BLAST of Carg12428 vs. ExPASy Swiss-Prot
Match: Q9LV09 (Protein BOBBER 1 OS=Arabidopsis thaliana OX=3702 GN=BOB1 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.9e-12
Identity = 41/160 (25.62%), Postives = 73/160 (45.62%), Query Frame = 0


Query: 19  EVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSS 78
           E + W Q L+EV + I +P    ++   C+I+   +++G+KG  P ++ EL   VK D  
Sbjct: 145 ENYSWIQNLQEVTVNIPVPTGTKARTVVCEIKKNRLKVGLKGQDPIVDGELYRSVKPDDC 204

Query: 79  FWTLED-GIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLE------------------- 138
           +W +ED  ++ I L K D+ + W   ++G  ++D    + E                   
Sbjct: 205 YWNIEDQKVISILLTKSDQMEWWKCCVKGEPEIDTQKVEPETSKLGDLDPETRSTVEKMM 264

Query: 139 ----------------QKRLMLQRFQEENPGFDFSQAQFS 143
                           QK+ +L++F  E+P  DFS A+F+
Sbjct: 265 FDQRQKQMGLPTSEELQKQEILKKFMSEHPEMDFSNAKFN 304

[Close]

BLAST of Carg12428 vs. ExPASy TrEMBL
Match: A0A6J1GA51 (nudC domain-containing protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452345 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 6.4e-90
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG G+LDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGRLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. ExPASy TrEMBL
Match: A0A6J1K923 (nudC domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492785 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 7.8e-88
Identity = 152/158 (96.20%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSF+HNGQ+VFEWDQTLEEVN+YINLPPNVHSKQFYCKIQSRHVELGIK 
Sbjct: 1   MAEKLAPEKRHSFIHNGQKVFEWDQTLEEVNIYINLPPNVHSKQFYCKIQSRHVELGIKR 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG GQLDPYSTDLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFS+AQFSGNCPDPRTFMGGIHSD
Sbjct: 121 RLMLQRFQEENPGFDFSKAQFSGNCPDPRTFMGGIHSD 158

[Close]

BLAST of Carg12428 vs. ExPASy TrEMBL
Match: A0A6J1DD50 (nudC domain-containing protein 2 OS=Momordica charantia OX=3673 GN=LOC111018990 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.5e-86
Identity = 150/158 (94.94%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           M EKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCK+QSRHVE+GIKG
Sbjct: 1   MEEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKVQSRHVEVGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNH+LT PVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQG GQLDPYSTDLEQK
Sbjct: 61  NPPYLNHDLTGPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGQGQLDPYSTDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQF+GNCPDPRTFMGGI SD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFTGNCPDPRTFMGGIRSD 158

[Close]

BLAST of Carg12428 vs. ExPASy TrEMBL
Match: A0A0A0LV57 (CS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530660 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 9.6e-86
Identity = 150/155 (96.77%), Postives = 153/155 (98.71%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVN+YINLPPNV+SKQFYCKIQSRHVELGIKG
Sbjct: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNIYINLPPNVNSKQFYCKIQSRHVELGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNHELTCPVKTDSSFWTLED IMHITLQKRDKGQTWASPIQG GQLDPYS+DLEQK
Sbjct: 61  NPPYLNHELTCPVKTDSSFWTLEDDIMHITLQKRDKGQTWASPIQGQGQLDPYSSDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGI 156
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGI
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGI 155

[Close]

BLAST of Carg12428 vs. ExPASy TrEMBL
Match: A0A445AMU9 (CS domain-containing protein OS=Arachis hypogaea OX=3818 GN=Ahy_B01g051824 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 4.7e-85
Identity = 146/158 (92.41%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRH+F+H+GQ+VFEWDQTLEEVN+YINLPPNVHSKQFYCKIQS+HVE+GIKG
Sbjct: 1   MAEKLAPEKRHNFVHDGQKVFEWDQTLEEVNIYINLPPNVHSKQFYCKIQSKHVEVGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNH+LTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPI G GQLDPY+TDLEQK
Sbjct: 61  NPPYLNHDLTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPILGQGQLDPYATDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGI SD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIRSD 158

[Close]

BLAST of Carg12428 vs. TAIR 10
Match: AT5G58740.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 2.0e-83
Identity = 137/158 (86.71%), Postives = 148/158 (93.67%), Query Frame = 0


Query: 1   MAEKLAPEKRHSFLHNGQEVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKG 60
           MAEKLAPEKRH F+HNGQ+VFEWDQTLEEVNMYI LPPNVH K F+CKIQS+H+E+GIKG
Sbjct: 1   MAEKLAPEKRHDFIHNGQKVFEWDQTLEEVNMYITLPPNVHPKSFHCKIQSKHIEVGIKG 60

Query: 61  NPPYLNHELTCPVKTDSSFWTLEDGIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLEQK 120
           NPPYLNH+L+ PVKTD SFWTLED IMHITLQKR+KGQTWASPI G GQLDPY+TDLEQK
Sbjct: 61  NPPYLNHDLSAPVKTDCSFWTLEDDIMHITLQKREKGQTWASPILGQGQLDPYATDLEQK 120

Query: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRTFMGGIHSD 159
           RLMLQRFQEENPGFDFSQAQFSGNCPDPR+FMGGI SD
Sbjct: 121 RLMLQRFQEENPGFDFSQAQFSGNCPDPRSFMGGIRSD 158

[Close]

BLAST of Carg12428 vs. TAIR 10
Match: AT4G27890.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 5.7e-14
Identity = 44/160 (27.50%), Postives = 75/160 (46.88%), Query Frame = 0


Query: 19  EVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSS 78
           E + W Q L+EV + I +P    S+   C+I+   +++G+KG    ++ E    VK D  
Sbjct: 134 EKYSWGQNLQEVTINIPMPEGTKSRSVTCEIKKNRLKVGLKGQDLIVDGEFFNSVKPDDC 193

Query: 79  FWTLED-GIMHITLQKRDKGQTWASPIQGP---------------GQLDPYS-------- 138
           FW +ED  ++ + L K+D+ + W   ++G                G LDP +        
Sbjct: 194 FWNIEDQKMISVLLTKQDQMEWWKYCVKGEPEIDTQKVEPETSKLGDLDPETRASVEKMM 253

Query: 139 ------------TDLEQKRLMLQRFQEENPGFDFSQAQFS 143
                       +D  +K+ ML++F  +NPG DFS A+F+
Sbjct: 254 FDQRQKQMGLPRSDEIEKKDMLKKFMAQNPGMDFSNAKFN 293

[Close]

BLAST of Carg12428 vs. TAIR 10
Match: AT5G53400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-13
Identity = 41/160 (25.62%), Postives = 73/160 (45.62%), Query Frame = 0


Query: 19  EVFEWDQTLEEVNMYINLPPNVHSKQFYCKIQSRHVELGIKGNPPYLNHELTCPVKTDSS 78
           E + W Q L+EV + I +P    ++   C+I+   +++G+KG  P ++ EL   VK D  
Sbjct: 145 ENYSWIQNLQEVTVNIPVPTGTKARTVVCEIKKNRLKVGLKGQDPIVDGELYRSVKPDDC 204

Query: 79  FWTLED-GIMHITLQKRDKGQTWASPIQGPGQLDPYSTDLE------------------- 138
           +W +ED  ++ I L K D+ + W   ++G  ++D    + E                   
Sbjct: 205 YWNIEDQKVISILLTKSDQMEWWKCCVKGEPEIDTQKVEPETSKLGDLDPETRSTVEKMM 264

Query: 139 ----------------QKRLMLQRFQEENPGFDFSQAQFS 143
                           QK+ +L++F  E+P  DFS A+F+
Sbjct: 265 FDQRQKQMGLPTSEELQKQEILKKFMSEHPEMDFSNAKFN 304

The following BLAST results are available for this feature:

BLAST of Carg12428 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036985.1	5.4e-91	100.00	NudC domain-containing protein 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6607306.1	4.5e-90	99.37	NudC domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022948776.1	1.3e-89	98.73	nudC domain-containing protein 2-like [Cucurbita moschata]	[more]
XP_023523271.1	5.0e-89	98.10	nudC domain-containing protein 2-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022998001.1	1.6e-87	96.20	nudC domain-containing protein 2-like [Cucurbita maxima]	[more]

BLAST of Carg12428 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8WVJ2	4.7e-21	40.00	NudC domain-containing protein 2 OS=Homo sapiens OX=9606 GN=NUDCD2 PE=1 SV=1	[more]
Q9CQ48	4.7e-21	40.00	NudC domain-containing protein 2 OS=Mus musculus OX=10090 GN=Nudcd2 PE=1 SV=1	[more]
Q5M823	7.9e-21	42.06	NudC domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Nudcd2 PE=2 SV...	[more]
Q9STN7	8.0e-13	27.50	Protein BOBBER 2 OS=Arabidopsis thaliana OX=3702 GN=BOB2 PE=2 SV=1	[more]
Q9LV09	3.9e-12	25.63	Protein BOBBER 1 OS=Arabidopsis thaliana OX=3702 GN=BOB1 PE=1 SV=1	[more]

BLAST of Carg12428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GA51	6.4e-90	98.73	nudC domain-containing protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452...	[more]
A0A6J1K923	7.8e-88	96.20	nudC domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149278...	[more]
A0A6J1DD50	2.5e-86	94.94	nudC domain-containing protein 2 OS=Momordica charantia OX=3673 GN=LOC111018990 ...	[more]
A0A0A0LV57	9.6e-86	96.77	CS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530660 PE=4 SV=...	[more]
A0A445AMU9	4.7e-85	92.41	CS domain-containing protein OS=Arachis hypogaea OX=3818 GN=Ahy_B01g051824 PE=4 ...	[more]

BLAST of Carg12428 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58740.1	2.0e-83	86.71	HSP20-like chaperones superfamily protein	[more]
AT4G27890.1	5.7e-14	27.50	HSP20-like chaperones superfamily protein	[more]
AT5G53400.1	2.8e-13	25.63	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007052	CS domain	PFAM	PF04969	CS	coord: 21..93 e-value: 1.9E-16 score: 60.9
IPR007052	CS domain	PROSITE	PS51203	CS	coord: 16..104 score: 15.523032
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 16..102 e-value: 5.7E-21 score: 76.9
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 10..114
None	No IPR available	GENE3D	1.20.5.740	Single helix bin	coord: 103..131 e-value: 4.4E-5 score: 25.2
None	No IPR available	PANTHER	PTHR12356:SF18	HSP20-LIKE CHAPERONES SUPERFAMILY PROTEIN	coord: 2..148
None	No IPR available	CDD	cd06467	p23_NUDC_like	coord: 21..104 e-value: 3.01749E-35 score: 116.108
IPR037898	NudC family	PANTHER	PTHR12356	NUCLEAR MOVEMENT PROTEIN NUDC	coord: 2..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12428-RA	Carg12428-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051082	unfolded protein binding