Carg12407 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12407
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerases I and III subunit RPAC2
Location	Carg_Chr01: 3407721 .. 3411535 (+)
RNA-Seq Expression	Carg12407
Synteny	Carg12407

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACGGCGGAGAGGTGAGGAGGCCCAGGTGGAGGAGCAAGGTTTATGAGAGTTAGGGTTTATGGTTAAGAGCTGGGGTTTGGGCTGGGCTAGGAGAATGTATTTGGGATTTTTTTTTTTTAATCCATATTTTTTAATATTTATTAAACAAACATTTAAATTGTTAATTAAATTCCAAAAATAATAACAATTTTTTCAAAATTAAAATGTTGTCGGTATTTTTAAACTTAGATATTTAATTTTAAGGAAGTATTAAATATATTTTATTTAAATTCAAATTTTAAAAACTTTTAAAAGTTTATAAATATTTTTTTTATAGAAAATCTTTTATTAATTTAACTAATAAAATAAAAAATAAATAAAAGAGAGGGAAACCCCTAACTAGCTCCTTTTGTTTATATCATACACGCGACTTCTTTTTGTTTACGTCGTCTCCTCCTCCGTTTCAGCAGAGCGCCGCCGCTGCCGCTGCCGCCTTAATCTTCCGTTCGTGCCGCCGCCGTCTTATTCTTCCGTTCGTGCCGCCGTTTTATTCTTCCGTTCGTGCCGCCGCAGTCTCATTCGATTCCCTACCTCTTTTTGTTACTGGTATCCTCCCAATCTCCATCTATGTACTTTTTCTCGTTTGATAAATACAAATTAGGATCTTCTAATGAAAGAATGGGAAATAGTTTGGTTTTTTTTTTTTGTTTTTTTGTTTTTTCTTACAATAAATAGCCAAATAGCAAAGGAGAAGAATAGCCTTGCGGCAAGTTGAGACGTAGTTTTTGGGTTGGCAGTGAATCAGTCACCTAATTCTCCTTTAATTCATAGCTTGAATGATTGACCGCAAATTCTAATTGGTTAACTACATTAATTTACCAAATTAGTTTGCACCAAAATTATAAACAAAAATCAGTAGCCATTGTTCTCCAATGCTCTTTCTAATATCTAATTTTCCTTGCTGTTGCTCCTTCACTCATTATTTGTAACTTTACCTTCTTATGTCCCTTGCTATGTTCTTCGCCTTTGAAATTTTTATATATAGAGATAGAGAGAATCATGAAGTTAAATAGTTTGACAAGTCTAATAGGATTTTATCGTTTCAGTTAACTCACCTTAATAATTATGGGTAGAACACAATAAATTCATAATTTTTATCTTATTTAATAAAAAACAATAGACAAAAGGTATACAATTCATTTTATAGAACACCATTTTATAGAATGATAAATAAATGAAAAACATAAGAAAGAACAATTTAAAGGAAGAAAAAATGAATAGGGCAAGTCCGGGAGAGAGTTTCTTTCCTATAGTCACTTTTATATCTATAGTATAGATTAGAGCAAAGATGTGTGAAGAACGTATGATGCCTTGAAAAGGGTAGTCAATAATCACGAAAGTCGTCTAGCTAATGCCATGTGCCTGCACTTGTGTTGGCTCTCGAAGATTAAACCAAATATTATAGGGTCAAATAGTTGTTTGATAAGATTGGTAGAGGTGCATACAAGCTAGTCCCGATACTTGTCGATATCGGAACAAAACTAATAATAAAACTTTCAAAGATTGAAATTTCATTTCTTTGGAGTTGGTGGAAGAGAATTCTTAACGTCTTGGCTTTGTTGGTTTTGGATAATTTTAGCCCTTTTATTCTATATTGTCAATTTTTTATGGGTTTCGTAGACTTTTAGTTTACTTATGTTATTTTCTTTGATTTAGTGTATTTGATTTTAAATTGAGACTCTGACCTAATTTTTCTTTATAGCCATGGAACACGGGTCCTTCACGGATGAGAGTAAATCAACATTTTCTATGGCTGATGAAGATCACACACTTGCAAATTCCATCAGATACACTTTGAATCAAGAGTACGAGCTCTTTTCCAGTTATTCTTGAGTGAATATTTGAACTGTTAACAAGACGTGCCAAAGAATTTGTAGTTCTTGGGCCACTGATCTTATCTAACCATTTCATCATTTTTCCAAAGATCATTGACTTCTATTAATTTGATGACATCAAGGATCAATTAGGTTCTTGGTTAACTCATCGAGCATAAATTGATGAATTGTGTACACCCATTGCATGTGTTTATATAGGTTCTTTCATCGTTCATTCAGAATAGTTGTTTATATTGTTGTCATCTTTCTTCACCTGTTCAAGCGATCCTCTCGACAGTTTTTTTTTTTTTCTTAAACAAACCTGGTTTAGATTGTTTGTTATTTTTCCTTTCATAAGAGTTATTTATATTGTTATTTTTTAAGTAAATCATATCCGTTATACTTTTAAATGACTCATAACATGATCTTGCAGTCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAACATCAGGGTCCAAACAACAGGTAAGTTTTTTGTGGTATTCTGATTCTGGAAAAGTACAGATCAGTTCCAATTATTTAGGACAGTTTTCTGATAATTAGATTTGTTTATTTTGCTTGGTTTCTTCTTTCTACACTCATGCAATTTATGCATTCATGACATAAATAGTCTGGATATATGCTGAGTGCGACCTAGGGAGAAAAATCTTTCCATAAAATCGACATATGTTGCCTGTTTTCTTTTTATTAATATGGTTTATAAAGAATATGTTTTAGTACTTAGAACTTTGGATGGAGTGCATTTAGTTTCAAGTTTCAATGTTTTGTCCAAATTTTGAAAAAAATTCTATGTAGTTCATACATCAAACACTAATAACTAGACGGTGTGACAATGCCCTATTAAACATACATTAGTTGATTCGACGTGCCATGAAAAATAGTAGAAGTGAATACTAGACAGTGTTTGGTAAGTTGTTTAAAAAAGAATAATATACATTTTATTTTGTTTAATTGTCAAGTTCTATCTACAATGTGTCAAATCAACCAAGATTTATCTAATAGGGCTTGCCACATTGTTACTTAGTTAGAAATGTTGATAGGCATGACTATTTAGAATGAATTGCAACTCAAAGCTTTAATGGCATAATCAAAACTTTTTCAAATCTAAAGACTAAATAGATACGACTATCGAAATATAAGTAGCCACCTATGTAGATTTTAACATCCTACGAGTTTCCTTAGCAACCAAAGGTAGTGGGGTTAGGTGGTTGTCCTATAAGATCAGTTGAGGCGAATGCAAGTTGACTCAGACCCTAGATAGATGTTTTTGAGGCCTAGATAGATGGTATCCAGAATGTATGGTGCTTCGCCAAAATTGGAGGTGGGAAAACAATGTCCAATGCTCTGTTTGAAACTAGCAAAAGCTAAATAATGGGGAAAGACAAAATCACATGTAAATGGTGGTGCAACAGCAAACTATTTGTTGAAGTGACCATGCATTAAGCATTAACATTAGGATCAGGGCTTATTACTATTTGTGGTAGTGATGGAAGAACTGGGTTTTGAATGTTGATTGAACTGAACTGAACTGAACATGGCAGGTGATCCAGCAAGAGAGGTTTTGAAAGATGCGTGTCAGAACTTGATGGCGGTGTGCCAGCATGTGAGGAACACACTTGACAAAGCTGTTCTTGATCACAAACTCACCAAGCCTGTGGGAGACATTAATATCAAATAGAGTGGGACCTTTCCTTCCCCCCTACTTTGTTGGGCTTATAACTTTCTTTTTTGCTCCATTTATATTATCGTTTACAGATTATTTGAGTCAGTTTAGTTGATGAATCTTTCTTACTTGTACTGTTATCTCAACTGATGCCCCCTCTCAGGGATTTTTGGTTAGATATTAAATACCGTAGATTTTACAACTTGGAAAATGCCAGATATTTGTAAGCTTCCTAAGATGTTTGATC

mRNA sequence

ATGGCGGACGGCGGAGAGCAGAGCGCCGCCGCTGCCGCTGCCGCCTTAATCTTCCGTTCGTGCCGCCGCCGTCTTATTCTTCCGTTCGTGCCGCCGTTTTATTCTTCCGTTCGTGCCGCCGCAGTCTCATTCGATTCCCTACCTCTTTTTGTTACTGCCATGGAACACGGGTCCTTCACGGATGAGAGTAAATCAACATTTTCTATGGCTGATGAAGATCACACACTTGCAAATTCCATCAGATACACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAACATCAGGGTCCAAACAACAGGTGATCCAGCAAGAGAGGTTTTGAAAGATGCGTGTCAGAACTTGATGGCGGTGTGCCAGCATGTGAGGAACACACTTGACAAAGCTGTTCTTGATCACAAACTCACCAAGCCTGTGGGAGACATTAATATCAAATAGAGTGGGACCTTTCCTTCCCCCCTACTTTGTTGGGCTTATAACTTTCTTTTTTGCTCCATTTATATTATCGTTTACAGATTATTTGAGTCAGTTTAGTTGATGAATCTTTCTTACTTGTACTGTTATCTCAACTGATGCCCCCTCTCAGGGATTTTTGGTTAGATATTAAATACCGTAGATTTTACAACTTGGAAAATGCCAGATATTTGTAAGCTTCCTAAGATGTTTGATC

Coding sequence (CDS)

ATGGCGGACGGCGGAGAGCAGAGCGCCGCCGCTGCCGCTGCCGCCTTAATCTTCCGTTCGTGCCGCCGCCGTCTTATTCTTCCGTTCGTGCCGCCGTTTTATTCTTCCGTTCGTGCCGCCGCAGTCTCATTCGATTCCCTACCTCTTTTTGTTACTGCCATGGAACACGGGTCCTTCACGGATGAGAGTAAATCAACATTTTCTATGGCTGATGAAGATCACACACTTGCAAATTCCATCAGATACACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAACATCAGGGTCCAAACAACAGGTGATCCAGCAAGAGAGGTTTTGAAAGATGCGTGTCAGAACTTGATGGCGGTGTGCCAGCATGTGAGGAACACACTTGACAAAGCTGTTCTTGATCACAAACTCACCAAGCCTGTGGGAGACATTAATATCAAATAG

Protein sequence

MADGGEQSAAAAAAALIFRSCRRRLILPFVPPFYSSVRAAAVSFDSLPLFVTAMEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK

Homology

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BLAST of Carg12407 vs. NCBI nr
Match: KAG7036964.1 (DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 314.3 bits (804), Expect = 5.9e-82
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 0


Query: 1   MADGGEQSAAAAAAALIFRSCRRRLILPFVPPFYSSVRAAAVSFDSLPLFVTAMEHGSFT 60
           MADGGEQSAAAAAAALIFRSCRRRLILPFVPPFYSSVRAAAVSFDSLPLFVTAMEHGSFT
Sbjct: 1   MADGGEQSAAAAAAALIFRSCRRRLILPFVPPFYSSVRAAAVSFDSLPLFVTAMEHGSFT 60

Query: 61  DESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVL 120
           DESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVL
Sbjct: 61  DESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVL 120

Query: 121 KDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           KDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK
Sbjct: 121 KDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 157

[Close]

BLAST of Carg12407 vs. NCBI nr
Match: XP_022948356.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita moschata] >XP_022998825.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita maxima] >KAG6607286.1 DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 217.6 bits (553), Expect = 7.5e-53
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Carg12407 vs. NCBI nr
Match: XP_023522054.1 (DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp. pepo] >XP_023524948.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.5 bits (550), Expect = 1.7e-52
Identity = 103/104 (99.04%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDE+KSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

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BLAST of Carg12407 vs. NCBI nr
Match: XP_038893936.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida])

HSP 1 Score: 207.2 bits (526), Expect = 1.0e-49
Identity = 98/104 (94.23%), Postives = 101/104 (97.12%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDE+KSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLM VCQHVRNT DKAVLD KLTKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDIKLTKPVGDINIK 104

[Close]

BLAST of Carg12407 vs. NCBI nr
Match: KAE8652742.1 (hypothetical protein Csa_014245 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 1.9e-48
Identity = 97/108 (89.81%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 50  FVTAMEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRV 109
           F  AMEHGSFTDE+KSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRV
Sbjct: 93  FCGAMEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRV 152

Query: 110 QTTGDPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           QTTGDPAREVLKDACQNLM VCQHVRNT DKAVLD KLT PV DINIK
Sbjct: 153 QTTGDPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 200

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BLAST of Carg12407 vs. ExPASy Swiss-Prot
Match: Q5XK67 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 GN=polr1d PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.1e-17
Identity = 42/90 (46.67%), Postives = 60/90 (66.67%), Query Frame = 0


Query: 61  DESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 120
           +ES  TF + +EDHTL NS+RY + ++P V FCGYSI HPS+ ++N R+QT  G PA E 
Sbjct: 20  EESCVTFVLHEEDHTLGNSLRYMVMKNPEVEFCGYSITHPSETKINFRIQTRNGIPAVEP 79

Query: 121 LKDACQNLMAVCQHVRNTLDKAVLDHKLTK 150
            +     L+ VCQHV NT + ++ ++K  K
Sbjct: 80  FRRGLNELVDVCQHVLNTFETSIKEYKEQK 109

[Close]

BLAST of Carg12407 vs. ExPASy Swiss-Prot
Match: P0DPB6 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=POLR1D PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.4e-16
Identity = 40/94 (42.55%), Postives = 60/94 (63.83%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           M   + TD    TF + +EDHTL NS+RY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 114 D-PAREVLKDACQNLMAVCQHVRNTLDKAVLDHK 147
             PA E  +     LM VCQHV +  + ++ D+K
Sbjct: 89  TLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYK 122

[Close]

BLAST of Carg12407 vs. ExPASy Swiss-Prot
Match: Q1RMG8 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Bos taurus OX=9913 GN=POLR1D PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 39/94 (41.49%), Postives = 61/94 (64.89%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           M   + TD    TF + +EDHTL NS+RY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 114 D-PAREVLKDACQNLMAVCQHVRNTLDKAVLDHK 147
             PA E  +    +LM VCQHV +  + ++ ++K
Sbjct: 89  ALPAVEPFQRGLTDLMNVCQHVLDKFEASIKEYK 122

[Close]

BLAST of Carg12407 vs. ExPASy Swiss-Prot
Match: Q6DRI4 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=polr1d PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-15
Identity = 40/87 (45.98%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 61  DESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 120
           DE   TF + +EDHTL NS+RY + +   V FCGYSI HPS++++N R+QT  G PA E 
Sbjct: 19  DEGCVTFVLNEEDHTLGNSLRYMIMKSQDVEFCGYSITHPSESKINFRIQTRDGVPASEP 78

Query: 121 LKDACQNLMAVCQHVRNTLDKAVLDHK 147
           L++   NL  VC+HV  T +  + + K
Sbjct: 79  LRNGLNNLTDVCKHVLQTFEARMKEFK 105

[Close]

BLAST of Carg12407 vs. ExPASy Swiss-Prot
Match: P97304 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN=Polr1d PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-15
Identity = 39/97 (40.21%), Postives = 59/97 (60.82%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           M   + TD    TF + +EDHTL N +RY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRQCVTFVLHEEDHTLGNCLRYIIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 114 D-PAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTK 150
             PA E  +     L+ VCQHV    + ++ D+K  K
Sbjct: 89  ALPAVEPFQKGLNELLNVCQHVLVKFEASIKDYKAKK 125

[Close]

BLAST of Carg12407 vs. ExPASy TrEMBL
Match: A0A6J1KDK3 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661 GN=LOC111493371 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.6e-53
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Carg12407 vs. ExPASy TrEMBL
Match: A0A6J1G901 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=3662 GN=LOC111452057 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.6e-53
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Carg12407 vs. ExPASy TrEMBL
Match: A0A6J1D9Q8 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3673 GN=LOC111018936 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.6e-48
Identity = 95/104 (91.35%), Postives = 100/104 (96.15%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSF+DESKSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFSDESKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           D AREVLKD+CQNLM VCQHVRNT DK+VLD KLTKPVGDINIK
Sbjct: 61  DSAREVLKDSCQNLMVVCQHVRNTFDKSVLDFKLTKPVGDINIK 104

[Close]

BLAST of Carg12407 vs. ExPASy TrEMBL
Match: A0A0A0LUT5 (RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.6e-48
Identity = 95/104 (91.35%), Postives = 99/104 (95.19%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDE+KSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 158
           DPAREVLKDACQNLM VCQHVRNT DKAVLD KLT PV DINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 104

[Close]

BLAST of Carg12407 vs. ExPASy TrEMBL
Match: A0A0A0LV21 (RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G527910 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.7e-47
Identity = 96/108 (88.89%), Postives = 99/108 (91.67%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFTDE+KSTFSMADEDHTLAN++R TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLT----KPVGDINIK 158
           DPAREVLKDACQNLM VCQHVRNT DKAVLD  LT    KPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 108

[Close]

BLAST of Carg12407 vs. TAIR 10
Match: AT2G29540.1 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 134.4 bits (337), Expect = 7.8e-32
Identity = 61/93 (65.59%), Postives = 78/93 (83.87%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFT+ S ++F++++EDHTLAN++R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHK 147
           DPAREV KDACQ LM + +HVR+  DKAV ++K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 93

[Close]

BLAST of Carg12407 vs. TAIR 10
Match: AT2G29540.2 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 134.4 bits (337), Expect = 7.8e-32
Identity = 61/93 (65.59%), Postives = 78/93 (83.87%), Query Frame = 0


Query: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113
           MEHGSFT+ S ++F++++EDHTLAN++R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHK 147
           DPAREV KDACQ LM + +HVR+  DKAV ++K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 93

[Close]

BLAST of Carg12407 vs. TAIR 10
Match: AT2G29540.3 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 87.0 bits (214), Expect = 1.4e-17
Identity = 40/59 (67.80%), Postives = 47/59 (79.66%), Query Frame = 0


Query: 88  PRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMAVCQHVRNTLDKAVLDHK 147
           PRVT   Y+IPHPS  +VNIRVQTTGDPAREV KDACQ LM + +HVR+  DKAV ++K
Sbjct: 52  PRVTVAAYTIPHPSLEQVNIRVQTTGDPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 110

[Close]

BLAST of Carg12407 vs. TAIR 10
Match: AT3G52090.1 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-07
Identity = 23/79 (29.11%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 65  STFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPA-REVLKDA 124
           ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R+ TT   +  +    A
Sbjct: 31  ASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTSQSSPMQAYNQA 90

Query: 125 CQNLMAVCQHVRNTLDKAV 143
             +L     +++N  +  V
Sbjct: 91  INDLDKELDYLKNQFEAEV 109

[Close]

BLAST of Carg12407 vs. TAIR 10
Match: AT3G52090.2 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 45.4 bits (106), Expect = 4.8e-05
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 65  STFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDAC 124
           ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R     DP     KD  
Sbjct: 31  ASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVR-----DP-----KDWF 86

Query: 125 QNLMAV 131
           +NL  V
Sbjct: 91  RNLKVV 86

The following BLAST results are available for this feature:

BLAST of Carg12407 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036964.1	5.9e-82	100.00	DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyros...	[more]
XP_022948356.1	7.5e-53	100.00	DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita moschata] >XP_02...	[more]
XP_023522054.1	1.7e-52	99.04	DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp....	[more]
XP_038893936.1	1.0e-49	94.23	DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida]	[more]
KAE8652742.1	1.9e-48	89.81	hypothetical protein Csa_014245 [Cucumis sativus]	[more]

BLAST of Carg12407 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XK67	3.1e-17	46.67	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 G...	[more]
P0DPB6	3.4e-16	42.55	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=...	[more]
Q1RMG8	1.3e-15	41.49	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Bos taurus OX=9913 GN=PO...	[more]
Q6DRI4	1.7e-15	45.98	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=p...	[more]
P97304	2.9e-15	40.21	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN...	[more]

BLAST of Carg12407 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KDK3	3.6e-53	100.00	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1G901	3.6e-53	100.00	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=36...	[more]
A0A6J1D9Q8	1.6e-48	91.35	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3...	[more]
A0A0A0LUT5	4.6e-48	91.35	RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010...	[more]
A0A0A0LV21	6.7e-47	88.89	RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G527910...	[more]

BLAST of Carg12407 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29540.1	7.8e-32	65.59	RNApolymerase 14 kDa subunit	[more]
AT2G29540.2	7.8e-32	65.59	RNApolymerase 14 kDa subunit	[more]
AT2G29540.3	1.4e-17	67.80	RNApolymerase 14 kDa subunit	[more]
AT3G52090.1	6.7e-07	29.11	DNA-directed RNA polymerase, RBP11-like	[more]
AT3G52090.2	4.8e-05	36.36	DNA-directed RNA polymerase, RBP11-like	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036603	RNA polymerase, RBP11-like subunit	GENE3D	3.30.1360.10		coord: 52..149 e-value: 3.2E-33 score: 115.7
IPR036603	RNA polymerase, RBP11-like subunit	SUPERFAMILY	55257	RBP11-like subunits of RNA polymerase	coord: 61..142
IPR009025	DNA-directed RNA polymerase, RBP11-like dimerisation domain	PFAM	PF13656	RNA_pol_L_2	coord: 65..136 e-value: 5.9E-29 score: 99.7
None	No IPR available	PANTHER	PTHR13946	DNA-DIRECTED RNA POLYMERASE I,II,III	coord: 54..157
None	No IPR available	PANTHER	PTHR13946:SF37	SUBFAMILY NOT NAMED	coord: 54..157
IPR008193	DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site	PROSITE	PS01154	RNA_POL_L_13KD	coord: 69..100
IPR033898	DNA-directed RNA polymerases I and III subunit AC19	CDD	cd07029	RNAP_I_III_AC19	coord: 55..138 e-value: 2.13384E-43 score: 136.593

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12407-RA	Carg12407-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0046983	protein dimerization activity