Carg12404 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12404
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	18.5 kDa class I heat shock protein-like
Location	Carg_Chr01: 3398079 .. 3398531 (-)
RNA-Seq Expression	Carg12404
Synteny	Carg12404

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATCATTCCAATCGGCGGCCAAGACGGCCGTATTTCCAACGGTTCCTCTTCAAGCATCCTCAATCGCTTCCCGAAATTCCCCTTCCCTCTTGACTTATGGCACGATTTCCCTTTCCCTTCCTCAATCTCGCACGCCTTTCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGTCTCGACTGGACCGAGACGCCGAACGCGCATGTTCTGAGAGCCTCCCTTCCGGGCTTCCAAGGAGAAGACGTTCTCGTCGAGCTTCAGGATGATCGGATGCTTCAGATCAGCACCGATAGTGGCGGCTTTGTGAGTAGATTCAAGATTCCGGAGAGCGGGAAGATCGAGGAGTTGAGTGCCTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCTAAAGAAGACGATCAGAGCAGACGCGATGTTCGAGTGGTGGAGATCACCGGCGAGTAG

mRNA sequence

ATGTCGATCATTCCAATCGGCGGCCAAGACGGCCGTATTTCCAACGGTTCCTCTTCAAGCATCCTCAATCGCTTCCCGAAATTCCCCTTCCCTCTTGACTTATGGCACGATTTCCCTTTCCCTTCCTCAATCTCGCACGCCTTTCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGTCTCGACTGGACCGAGACGCCGAACGCGCATGTTCTGAGAGCCTCCCTTCCGGGCTTCCAAGGAGAAGACGTTCTCGTCGAGCTTCAGGATGATCGGATGCTTCAGATCAGCACCGATAGTGGCGGCTTTGTGAGTAGATTCAAGATTCCGGAGAGCGGGAAGATCGAGGAGTTGAGTGCCTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCTAAAGAAGACGATCAGAGCAGACGCGATGTTCGAGTGGTGGAGATCACCGGCGAGTAG

Coding sequence (CDS)

ATGTCGATCATTCCAATCGGCGGCCAAGACGGCCGTATTTCCAACGGTTCCTCTTCAAGCATCCTCAATCGCTTCCCGAAATTCCCCTTCCCTCTTGACTTATGGCACGATTTCCCTTTCCCTTCCTCAATCTCGCACGCCTTTCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGTCTCGACTGGACCGAGACGCCGAACGCGCATGTTCTGAGAGCCTCCCTTCCGGGCTTCCAAGGAGAAGACGTTCTCGTCGAGCTTCAGGATGATCGGATGCTTCAGATCAGCACCGATAGTGGCGGCTTTGTGAGTAGATTCAAGATTCCGGAGAGCGGGAAGATCGAGGAGTTGAGTGCCTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCTAAAGAAGACGATCAGAGCAGACGCGATGTTCGAGTGGTGGAGATCACCGGCGAGTAG

Protein sequence

MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE

Homology

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BLAST of Carg12404 vs. NCBI nr
Match: XP_022949250.1 (18.5 kDa class I heat shock protein-like [Cucurbita moschata] >XP_022998185.1 18.5 kDa class I heat shock protein-like [Cucurbita maxima] >KAG7036961.1 18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.1 bits (793), Expect = 1.1e-80
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPKEDDQSRRDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

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BLAST of Carg12404 vs. NCBI nr
Match: KAG6607282.1 (18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.8 bits (787), Expect = 5.3e-80
Identity = 149/150 (99.33%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISN SSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR
Sbjct: 1   MSIIPIGGQDGRISNSSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPKEDDQSRRDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

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BLAST of Carg12404 vs. NCBI nr
Match: XP_023524947.1 (18.5 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.8 bits (774), Expect = 1.7e-78
Identity = 146/150 (97.33%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISN SSSSI+NRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR
Sbjct: 1   MSIIPIGGQDGRISNSSSSSIVNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRA LPGFQGEDVLVELQDDRMLQIST+SGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRAFLPGFQGEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPKEDDQSRRDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

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BLAST of Carg12404 vs. NCBI nr
Match: ADU55785.1 (HSP16.5 [Citrullus lanatus])

HSP 1 Score: 267.7 bits (683), Expect = 6.1e-68
Identity = 133/151 (88.08%), Postives = 141/151 (93.38%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISN S S+ILNRFP FPFPLDLWHDFPFPSSIS     F++GG+VNTR
Sbjct: 1   MSIIPIGGQDGRISNTSPSNILNRFPNFPFPLDLWHDFPFPSSIS---DPFSWGGTVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGF GEDVLVELQDDRMLQIST+SGGFVSRFKIPE+GKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFGGEDVLVELQDDRMLQISTESGGFVSRFKIPETGKIEELSA 120

Query: 121 FMDFGVLTVFVPKE-DDQSRRDVRVVEITGE 151
           FMDFG+LTVFVPKE DD+SRRDVRVVEITGE
Sbjct: 121 FMDFGILTVFVPKEDDDRSRRDVRVVEITGE 148

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BLAST of Carg12404 vs. NCBI nr
Match: XP_008457423.1 (PREDICTED: 17.5 kDa class I heat shock protein-like [Cucumis melo] >TYJ97356.1 17.5 kDa class I heat shock protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 258.5 bits (659), Expect = 3.7e-65
Identity = 130/151 (86.09%), Postives = 137/151 (90.73%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPI GQDGR+SN S S+ILNRFP FPFPLDLWHDFPFPSSIS     F++G +VNTR
Sbjct: 1   MSIIPITGQDGRVSNPSPSNILNRFPNFPFPLDLWHDFPFPSSIS---DPFSWGATVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGF  EDVLVELQDDRMLQIST+SGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPK-EDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPK EDD+S RDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKDEDDRSGRDVRVVEITGE 148

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BLAST of Carg12404 vs. ExPASy Swiss-Prot
Match: P05478 (18.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP18.5-C PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.7e-26
Identity = 64/145 (44.14%), Postives = 95/145 (65.52%), Query Frame = 0


Query: 29  PFPLDLW---HDFPFPSSISHA-FPDFAFGGS--VNTRLDWTETPNAHVLRASLPGFQGE 88
           PF LD+W    DFPFP+++S A FP+F+   S  V+TR+DW ETP AHV +A +PG + E
Sbjct: 18  PFSLDVWDPFKDFPFPNTLSSASFPEFSRENSAFVSTRVDWKETPEAHVFKADIPGLKKE 77

Query: 89  DVLVELQDDRMLQISTD------------------SGGFVSRFKIPESGKIEELSAFMDF 148
           +V V+++DD++LQIS +                  SG F+ RF++PE+ K+E++ A M+ 
Sbjct: 78  EVKVQIEDDKVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKVEQVKASMEN 137

Query: 149 GVLTVFVPKEDDQSRRDVRVVEITG 150
           GVLTV VPKE +  + DV+ +EI+G
Sbjct: 138 GVLTVTVPKE-EVKKPDVKAIEISG 161

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BLAST of Carg12404 vs. ExPASy Swiss-Prot
Match: P04793 (17.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.5-M PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.0e-25
Identity = 69/170 (40.59%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLW---HDFPFPSSISHAFPDFAFGGSV 60
           MS+IP       I  G  S++ +     PF LD+W    DF FP+S+S     F     V
Sbjct: 1   MSLIP------SIFGGRRSNVFD-----PFSLDVWDPFKDFHFPTSLSAENSAF-----V 60

Query: 61  NTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD------------------ 120
           NTR+DW ETP AHV  A +PG + E+V V+++DDR+LQIS +                  
Sbjct: 61  NTRVDWKETPEAHVFEADIPGLKKEEVKVQIEDDRVLQISGERNLEKEDKNDTWHRVERS 120

Query: 121 SGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDQSRRDVRVVEITG 150
           SG F+ RF++PE+ K+E++ A M+ GVLTV VPKE +  + DV+ +EI+G
Sbjct: 121 SGNFMRRFRLPENAKVEQVKASMENGVLTVTVPKE-EVKKPDVKAIEISG 153

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BLAST of Carg12404 vs. ExPASy Swiss-Prot
Match: P02519 (17.3 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.3-B PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.3e-24
Identity = 68/170 (40.00%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLW---HDFPFPSSISHAFPDFAFGGSV 60
           MS+IP          G  SS+ +     PF LD+W    DFPFPSS+S     F     V
Sbjct: 1   MSLIP------SFFGGRRSSVFD-----PFSLDVWDPFKDFPFPSSLSAENSAF-----V 60

Query: 61  NTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD------------------ 120
           +TR+DW ETP AHV +A +PG + E+V +E+QD R+LQIS +                  
Sbjct: 61  STRVDWKETPEAHVFKADIPGLKKEEVKLEIQDGRVLQISGERNVEKEDKNDTWHRVERS 120

Query: 121 SGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDQSRRDVRVVEITG 150
           SG  V RF++PE+ K++++ A M+ GVLTV VPKE+ + + DV+ ++I+G
Sbjct: 121 SGKLVRRFRLPENAKVDQVKASMENGVLTVTVPKEEIK-KPDVKAIDISG 153

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BLAST of Carg12404 vs. ExPASy Swiss-Prot
Match: P30693 (17.6 kDa class I heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.6 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-23
Identity = 59/139 (42.45%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 29  PFPLDLWHDFPFPSSISHAFPDFAFGGSVNTRLDWTETPNAHVLRASLPGFQGEDVLVEL 88
           PF LD W   PF   IS   P       VN R+DW ETP AHVL+A LPG + E+V VE+
Sbjct: 18  PFSLDTWD--PFQGIIS-TEPARETAAIVNARIDWKETPEAHVLKADLPGMKKEEVKVEV 77

Query: 89  QDDRMLQISTD------------------SGGFVSRFKIPESGKIEELSAFMDFGVLTVF 148
           +D R+LQIS +                  SG F+ RF++PE+ K++E+ A M+ GVLTV 
Sbjct: 78  EDGRVLQISGERCREQEEKDDTWHRVERSSGKFIRRFRLPENAKMDEVKAMMENGVLTVV 137

Query: 149 VPKEDDQSRRDVRVVEITG 150
           VPKE+++ +  V+ ++I+G
Sbjct: 138 VPKEEEEKKPMVKAIDISG 153

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BLAST of Carg12404 vs. ExPASy Swiss-Prot
Match: P27880 (18.2 kDa class I heat shock protein OS=Medicago sativa OX=3879 GN=HSP18.2 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.6e-23
Identity = 63/145 (43.45%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 29  PFPLDLW---HDFPF-PSSISHAFP--DFAFGGSVNTRLDWTETPNAHVLRASLPGFQGE 88
           PF LD+W    DFPF  S++S +FP  + AF   V+TR+DW ETP AHV +A LPG + E
Sbjct: 18  PFSLDVWDPFKDFPFNNSALSASFPRENSAF---VSTRVDWKETPEAHVFKADLPGMKKE 77

Query: 89  DVLVELQDDRMLQISTD------------------SGGFVSRFKIPESGKIEELSAFMDF 148
           +V VE++DDR+LQIS +                  SG F+ RF++PE+ K++++ A M+ 
Sbjct: 78  EVKVEIEDDRVLQISGERSVEKEDKNDQWHRLERSSGKFMRRFRLPENAKMDQVKAAMEN 137

Query: 149 GVLTVFVPKEDDQSRRDVRVVEITG 150
           GVLTV VPKE +  + +V+ ++I+G
Sbjct: 138 GVLTVTVPKE-EVKKPEVKTIDISG 158

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BLAST of Carg12404 vs. ExPASy TrEMBL
Match: A0A6J1KBV9 (18.5 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111492909 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 5.2e-81
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPKEDDQSRRDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Carg12404 vs. ExPASy TrEMBL
Match: A0A6J1GC89 (18.5 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111452661 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 5.2e-81
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPKEDDQSRRDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Carg12404 vs. ExPASy TrEMBL
Match: H6TB38 (HSP16.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.9e-68
Identity = 133/151 (88.08%), Postives = 141/151 (93.38%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPIGGQDGRISN S S+ILNRFP FPFPLDLWHDFPFPSSIS     F++GG+VNTR
Sbjct: 1   MSIIPIGGQDGRISNTSPSNILNRFPNFPFPLDLWHDFPFPSSIS---DPFSWGGTVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGF GEDVLVELQDDRMLQIST+SGGFVSRFKIPE+GKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFGGEDVLVELQDDRMLQISTESGGFVSRFKIPETGKIEELSA 120

Query: 121 FMDFGVLTVFVPKE-DDQSRRDVRVVEITGE 151
           FMDFG+LTVFVPKE DD+SRRDVRVVEITGE
Sbjct: 121 FMDFGILTVFVPKEDDDRSRRDVRVVEITGE 148

[Close]

BLAST of Carg12404 vs. ExPASy TrEMBL
Match: A0A5D3BEA9 (17.5 kDa class I heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001660 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.8e-65
Identity = 130/151 (86.09%), Postives = 137/151 (90.73%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPI GQDGR+SN S S+ILNRFP FPFPLDLWHDFPFPSSIS     F++G +VNTR
Sbjct: 1   MSIIPITGQDGRVSNPSPSNILNRFPNFPFPLDLWHDFPFPSSIS---DPFSWGATVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGF  EDVLVELQDDRMLQIST+SGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPK-EDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPK EDD+S RDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKDEDDRSGRDVRVVEITGE 148

[Close]

BLAST of Carg12404 vs. ExPASy TrEMBL
Match: A0A1S3C660 (17.5 kDa class I heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103497114 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.8e-65
Identity = 130/151 (86.09%), Postives = 137/151 (90.73%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MSIIPI GQDGR+SN S S+ILNRFP FPFPLDLWHDFPFPSSIS     F++G +VNTR
Sbjct: 1   MSIIPITGQDGRVSNPSPSNILNRFPNFPFPLDLWHDFPFPSSIS---DPFSWGATVNTR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEELSA 120
           LDWTETPNAHVLRASLPGF  EDVLVELQDDRMLQIST+SGGFVSRFKIPESGKIEELSA
Sbjct: 61  LDWTETPNAHVLRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEELSA 120

Query: 121 FMDFGVLTVFVPK-EDDQSRRDVRVVEITGE 151
           FMDFGVLTVFVPK EDD+S RDVRVVEITGE
Sbjct: 121 FMDFGVLTVFVPKDEDDRSGRDVRVVEITGE 148

[Close]

BLAST of Carg12404 vs. TAIR 10
Match: AT2G19310.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-22
Identity = 63/163 (38.65%), Postives = 92/163 (56.44%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNG----SSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGS 60
           MS+IPI  +  R+S G        ++N F  FP P  L+    FPS     FP  +   +
Sbjct: 1   MSMIPISNR-RRLSPGDRIWEPFELMNTFLDFPSPA-LFLSHHFPSLSREIFPQTS-SST 60

Query: 61  VNTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIE 120
           VNT+L+WTETP AHV +A LPG   ++V+  + ++  LQI T    F+SRFK+P +   +
Sbjct: 61  VNTQLNWTETPTAHVFKAYLPGVDQDEVIAFVDEEGYLQICTGDNKFMSRFKLPNNALTD 120

Query: 121 ELSAFMDFGVLTVFVPKEDDQS---------RRDVRVVEITGE 151
           +++A+M+   L VFV K+   S          R+VRVVEITG+
Sbjct: 121 QVTAWMEDEFLVVFVEKDASSSPPQLPEIEENRNVRVVEITGD 160

[Close]

BLAST of Carg12404 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.2e-21
Identity = 66/172 (38.37%), Postives = 95/172 (55.23%), Query Frame = 0


Query: 1   MSIIP-IGGQDGRISNGSSSSILNRFPKFPFPLDLWHDF---PFPSSISHAFPDFAFGGS 60
           MS+IP   G + RI+N       N F   PF LD+W  F    FPSS S A         
Sbjct: 1   MSLIPSFFGNNRRINN-------NIFD--PFSLDVWDPFKELQFPSSSSSAI-------- 60

Query: 61  VNTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD----------------- 120
            N R+DW ET  AHV +A LPG + E+V VE++DD +L+IS +                 
Sbjct: 61  ANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVER 120

Query: 121 -SGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPK-EDDQSRRDVRVVEITG 150
            SGGF  +F++PE+ K++++ A M+ GVLTV VPK E ++ +  V+ ++I+G
Sbjct: 121 SSGGFSRKFRLPENVKMDQVKASMENGVLTVTVPKVETNKKKAQVKSIDISG 155

[Close]

BLAST of Carg12404 vs. TAIR 10
Match: AT2G29500.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.0e-20
Identity = 61/167 (36.53%), Postives = 94/167 (56.29%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFPFPSSISHAFPDFAFGGSVNTR 60
           MS+IP        +N   S+I +     PF LD+W  F   +S S +  + A    VN R
Sbjct: 1   MSMIP-----SFFNNNRRSNIFD-----PFSLDVWDPFKELTSSSLSRENSAI---VNAR 60

Query: 61  LDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD------------------SGG 120
           +DW ETP AHV +A LPG + E+V VE+++D +L+IS +                  SG 
Sbjct: 61  VDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQ 120

Query: 121 FVSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDQSRRDVRVVEITG 150
           F  RF++PE+ K++++ A M+ GVLTV VPK + + + DV+ ++I+G
Sbjct: 121 FTRRFRLPENVKMDQVKAAMENGVLTVTVPKAETK-KADVKSIQISG 153

[Close]

BLAST of Carg12404 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 97.1 bits (240), Expect = 1.3e-20
Identity = 63/171 (36.84%), Postives = 92/171 (53.80%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDFP---FPSS-ISHAFPDFAFGGS 60
           MS+IP       I  G  S++ +     PF  DLW  F     PSS +++A         
Sbjct: 1   MSLIP------SIFGGRRSNVFD-----PFSQDLWDPFEGFFTPSSALANASTARDVAAF 60

Query: 61  VNTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD----------------- 120
            N R+DW ETP AHV +A LPG + E+V VE++D  +LQIS +                 
Sbjct: 61  TNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVER 120

Query: 121 -SGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDQSRRDVRVVEITG 150
            SG F+ RF++PE+ K+EE+ A M+ GVLTV VPK  ++ +  V+ ++I+G
Sbjct: 121 ASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEK-KPQVKSIDISG 159

[Close]

BLAST of Carg12404 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.7e-20
Identity = 64/171 (37.43%), Postives = 93/171 (54.39%), Query Frame = 0


Query: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHDF---PFPSSISHAFPDFAFGGSV 60
           MS+IP    + R SN    SI +     PF LD+W  F    FPSS+S            
Sbjct: 1   MSLIPSFFGNNRRSN----SIFD-----PFSLDVWDPFKELQFPSSLSG-----ETSAIT 60

Query: 61  NTRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTD------------------ 120
           N R+DW ET  AHV +A LPG + E+V VE++DD +L+IS +                  
Sbjct: 61  NARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERS 120

Query: 121 SGGFVSRFKIPESGKIEELSAFMDFGVLTVFVPK-EDDQSRRDVRVVEITG 150
           SG F  +FK+PE+ K++++ A M+ GVLTV VPK E+ + +  V+ ++I+G
Sbjct: 121 SGQFSRKFKLPENVKMDQVKASMENGVLTVTVPKVEEAKKKAQVKSIDISG 157

The following BLAST results are available for this feature:

BLAST of Carg12404 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949250.1	1.1e-80	100.00	18.5 kDa class I heat shock protein-like [Cucurbita moschata] >XP_022998185.1 18...	[more]
KAG6607282.1	5.3e-80	99.33	18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023524947.1	1.7e-78	97.33	18.5 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo]	[more]
ADU55785.1	6.1e-68	88.08	HSP16.5 [Citrullus lanatus]	[more]
XP_008457423.1	3.7e-65	86.09	PREDICTED: 17.5 kDa class I heat shock protein-like [Cucumis melo] >TYJ97356.1 1...	[more]

BLAST of Carg12404 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05478	2.7e-26	44.14	18.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP18.5-C PE=3 SV=...	[more]
P04793	5.0e-25	40.59	17.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.5-M PE=3 SV=...	[more]
P02519	4.3e-24	40.00	17.3 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.3-B PE=3 SV=...	[more]
P30693	2.8e-23	42.45	17.6 kDa class I heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.6 PE=2...	[more]
P27880	3.6e-23	43.45	18.2 kDa class I heat shock protein OS=Medicago sativa OX=3879 GN=HSP18.2 PE=2 S...	[more]

BLAST of Carg12404 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KBV9	5.2e-81	100.00	18.5 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1GC89	5.2e-81	100.00	18.5 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
H6TB38	2.9e-68	88.08	HSP16.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A5D3BEA9	1.8e-65	86.09	17.5 kDa class I heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3C660	1.8e-65	86.09	17.5 kDa class I heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103497114...	[more]

BLAST of Carg12404 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19310.1	3.1e-22	38.65	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	1.2e-21	38.37	HSP20-like chaperones superfamily protein	[more]
AT2G29500.1	1.0e-20	36.53	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	1.3e-20	36.84	heat shock protein 18.2	[more]
AT1G07400.1	1.7e-20	37.43	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 29..149 e-value: 4.5E-16 score: 60.9
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 27..144
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 62..147 e-value: 5.1E-8 score: 33.0
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 52..150 score: 14.172676
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 28..149
None	No IPR available	PANTHER	PTHR11527:SF320	17.5 KDA CLASS I HEAT SHOCK PROTEIN	coord: 28..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12404-RA	Carg12404-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding