Carg12391 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12391
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTropinone reductase-like protein
LocationCarg_Chr01: 3339960 .. 3342755 (+)
RNA-Seq ExpressionCarg12391
SyntenyCarg12391
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCAATCCAGACAAGGGCAGGCAGGAAAAACCATGGAAGGAGGGGTATTTAAGAAATTTCACATACATATAGGTAGAAGGTCCAAAAGGGCATTTAAAAAAAGTGGATCACTTTTGAAAATAACACAATTTGGGTTAATTTTGCAATTTTCGAAGGTGGTGTTGAAACTCGAATCGCAGACTGGCGAGACCTGCAACCTGTTGAGGCCATCCCCAAGAACAATGTTCTTGGCTCTTGTTTCTCCTTCACCCTTCGATTCATTCTCTTCTTCTACTTTCAATCCCTCACAGCCCCGTCTTTCAACATTTTCTTCATCTCCCATTTCCCGCTCCTTCAAACCTTTTTGGCTTCCCACATCAAATTCAACTCACAAACTCGTTAAACGCCTCAAATCCATTAGAAATCAAGCTGAATCCGACTTCAAAACCAAAAATGGCAGCACACCAGGCGACCATCGATGGTCTCTTCACGGAACGACGGCACTCGTTACCGGCGGCACTCGCGGAATCGGGTAATTTTGTATCTATGTTGACATATAAATTATCGTTTTAGATTGTTGGGTTTTGTTCCGCTGTTTTTGAGAATTTGGCAATGGTTTTTGGTTTAGGCGTGCGATTGTGGAGGAATTAGTGGGTTTTGGTGCGAGAGTGCATACTTGTTCTCGGAATGAGAATGAGCTGAAACAGTGTTTGAGGGATTGGAAGGATTTGGAGTTCGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGCTTATGAAGACTGTGGCTGATACTTTTGATGGGAAACTCAACATTCTTGTAAGGTGCACCTTTCTTGTGCTTTGCTTAGCTTTAGATTAAGCTGCATTTTACTTTGTTGGGTTACTTTCCTGTTCTCTCTTGTGCTTTCAATGGTTGTATAAGTGATATCAAAACTCACATGAGTCAATGCCAAGAACTAAGTCTAGGAAGCTACACGAATCCTTAGCTAACATGTGTAGAACCATTGGCTCAAGGAAGTGCAAATCATAGGATGAAGAAAGCTTCTATTCAACATGGGCTAAAACACGTTTCCATACATTGGAGGTGTAACGGCCCAATCCCACCGCTAGCAAATATTGTCATCTTTAGGCTTCCCCTCAAGGTTTTTAAAATGCTTTTGCTAGGGAGAGGTTTCCACACCCTTATAGAGAATCCTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAATCCACGTCCTTTGAGGTCTAGCTTCCTCACTGACACTTGTTCCCTTCTCCCATCAATGTGAGACCTCACAATCCACCCACTTCGGGGCCAGCGTCTTTTGCTGGTACACCATCTCGTGTCTACCCCCTTCGAGGCTCAACCTCCTTGCTGGCACATTGCTTAGAGTTTGGCTCTTATACCATTTGTAAAAGTCTAAGTCCACCGCTATTAGATATTGTCTTCTCTCGGCTTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGTTAGGGAGAGGTTTCCACACTCTTACAAAGAAGGTTTCGTTCTCCTACCCCAATCGATGTGGGATCTCACCGGAGGACTTCCTCCTTGAAGACGAGCAATTACCAAAGGCCAAATTCCATAAACAAGCTTTGGAAACCCACACACGAACCTTGGACATTTTGAGCTATCCTTTGAGCCGGATTCTAAATGTGTTTCTAAATTCCTTTAGGTTAGTTACTCACCCTCAAAGGCCTAGGAGGAAGCTTGTAGCTAGTAGGAGGCATGCCTAATTCGTACGTTTAAAATGGTTGGATATCTTGATGATGAAATGAATTTGAGAGGTTTTTCTATGAACCCATCTTCCAGCATCAAATGGGCAAATTCTAAATCTAAGAACAAAAAAAACACATTGATTTCATGATTGTTTCAATCCTTACAGACCATGAACACCATTTCTACGATCATCAATCATGTAGGTAAACAATGTGGGTAGAAATATTCGAAAGCCAACAACAGAGTTCACAGATGAAGAATTTTCTTTCCTTATGAAAACGAACGTCGAATCTGTCTTCCATTTATCTCAACTCGCTCATCCTTTTCTAAAAGCATCGGGCGCGGGGAGCATCGTGTTCATGTCCTCCGTGTCGAGCTTTGTCTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACAAAAGGTGATCGATCAGTGATCAATGTTACCTATGGTTTCCAAGTTTTATGGATGCAAACATTAGTTTCCTGAACTTTGTAATGACTCTTTCCAGGAGCTATCAATCAACTCACAAAATATTTAGCCTGTGAATGGGCAAACGACAACATAAGGAGCAACGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTAGAACAAGTAAATCTCGAATAACCCCACTTTGTTTGAACTCATGAATAATCACTCGTGATATTTGTCTCGCTTTACTCGTGCTATATATATCGTATCTTCTAGGTGTTGAGTAACAAAGCATACGTCGAAGAGGTATATTCTCGAACTCCACTTCGACGACTCGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTCGATGGAGGGATGTCTGTTAATGGCTTTGATCCAAGTTAACATTAGAAGAAACAGAGATATTGAATGTTTTTCTATATACTATTCTAGTGATCATTTGTTTGTTTAAGTGTCCAAATTATTGAATAAAGCTCGCCGGAACGATTGTAATATCTATCGGTATATATTCAAGATACATTTTAT

mRNA sequence

ATGTTGCAATCCAGACAAGGGCAGGCAGGAAAAACCATGGAAGGAGGGGTGGTGTTGAAACTCGAATCGCAGACTGGCGAGACCTGCAACCTGTTGAGGCCATCCCCAAGAACAATGTTCTTGGCTCTTGTTTCTCCTTCACCCTTCGATTCATTCTCTTCTTCTACTTTCAATCCCTCACAGCCCCGTCTTTCAACATTTTCTTCATCTCCCATTTCCCGCTCCTTCAAACCTTTTTGGCTTCCCACATCAAATTCAACTCACAAACTCGTTAAACGCCTCAAATCCATTAGAAATCAAGCTGAATCCGACTTCAAAACCAAAAATGGCAGCACACCAGGCGACCATCGATGGTCTCTTCACGGAACGACGGCACTCGTTACCGGCGGCACTCGCGGAATCGGGCGTGCGATTGTGGAGGAATTAGTGGGTTTTGGTGCGAGAGTGCATACTTGTTCTCGGAATGAGAATGAGCTGAAACAGTGTTTGAGGGATTGGAAGGATTTGGAGTTCGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGCTTATGAAGACTGTGGCTGATACTTTTGATGGGAAACTCAACATTCTTGTAAGACCTCACAATCCACCCACTTCGGGGCCAGCGTCTTTTGCTGGTACACCATCTCGTGTCTACCCCCTTCGAGGCTCAACCTCCTTGCTGGCACATTGCTTAGAGTTTGGCTCTTATACCATTTGTAAAAGTCTAAGTCCACCGCTATTAGATATTGTCTTCTCTCGGCTTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGTTAGGGAGAGGTTTCCACACTCTTACAAAGAAGGTTTCGTTCTCCTACCCCAATCGATGTGGGATCTCACCGGAGGACTTCCTCCTTGAAGACGAGCAATTACCAAAGGCCAAATTCCATAAACAAGCTTTGGAAACCCACACACGAACCTTGGACATTTTGAGCTATCCTTTGAGCCGGATTCTAAATGTGTTTCTAAATTCCTTTAGGTTAGTTACTCACCCTCAAAGGCCTAGGAGGAAGCTTGTAAACAATGTGGGTAGAAATATTCGAAAGCCAACAACAGAGTTCACAGATGAAGAATTTTCTTTCCTTATGAAAACGAACGTCGAATCTGTCTTCCATTTATCTCAACTCGCTCATCCTTTTCTAAAAGCATCGGGCGCGGGGAGCATCGTGTTCATGTCCTCCGTGTCGAGCTTTGTCTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACAAAAGGAGCTATCAATCAACTCACAAAATATTTAGCCTGTGAATGGGCAAACGACAACATAAGGAGCAACGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTAGAACAAGTGTTGAGTAACAAAGCATACGTCGAAGAGGTATATTCTCGAACTCCACTTCGACGACTCGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTCGATGGAGGGATGTCTGTTAATGGCTTTGATCCAAGTTAACATTAGAAGAAACAGAGATATTGAATGTTTTTCTATATACTATTCTAGTGATCATTTGTTTGTTTAAGTGTCCAAATTATTGAATAAAGCTCGCCGGAACGATTGTAATATCTATCGGTATATATTCAAGATACATTTTAT

Coding sequence (CDS)

ATGTTGCAATCCAGACAAGGGCAGGCAGGAAAAACCATGGAAGGAGGGGTGGTGTTGAAACTCGAATCGCAGACTGGCGAGACCTGCAACCTGTTGAGGCCATCCCCAAGAACAATGTTCTTGGCTCTTGTTTCTCCTTCACCCTTCGATTCATTCTCTTCTTCTACTTTCAATCCCTCACAGCCCCGTCTTTCAACATTTTCTTCATCTCCCATTTCCCGCTCCTTCAAACCTTTTTGGCTTCCCACATCAAATTCAACTCACAAACTCGTTAAACGCCTCAAATCCATTAGAAATCAAGCTGAATCCGACTTCAAAACCAAAAATGGCAGCACACCAGGCGACCATCGATGGTCTCTTCACGGAACGACGGCACTCGTTACCGGCGGCACTCGCGGAATCGGGCGTGCGATTGTGGAGGAATTAGTGGGTTTTGGTGCGAGAGTGCATACTTGTTCTCGGAATGAGAATGAGCTGAAACAGTGTTTGAGGGATTGGAAGGATTTGGAGTTCGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGCTTATGAAGACTGTGGCTGATACTTTTGATGGGAAACTCAACATTCTTGTAAGACCTCACAATCCACCCACTTCGGGGCCAGCGTCTTTTGCTGGTACACCATCTCGTGTCTACCCCCTTCGAGGCTCAACCTCCTTGCTGGCACATTGCTTAGAGTTTGGCTCTTATACCATTTGTAAAAGTCTAAGTCCACCGCTATTAGATATTGTCTTCTCTCGGCTTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGTTAGGGAGAGGTTTCCACACTCTTACAAAGAAGGTTTCGTTCTCCTACCCCAATCGATGTGGGATCTCACCGGAGGACTTCCTCCTTGAAGACGAGCAATTACCAAAGGCCAAATTCCATAAACAAGCTTTGGAAACCCACACACGAACCTTGGACATTTTGAGCTATCCTTTGAGCCGGATTCTAAATGTGTTTCTAAATTCCTTTAGGTTAGTTACTCACCCTCAAAGGCCTAGGAGGAAGCTTGTAAACAATGTGGGTAGAAATATTCGAAAGCCAACAACAGAGTTCACAGATGAAGAATTTTCTTTCCTTATGAAAACGAACGTCGAATCTGTCTTCCATTTATCTCAACTCGCTCATCCTTTTCTAAAAGCATCGGGCGCGGGGAGCATCGTGTTCATGTCCTCCGTGTCGAGCTTTGTCTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACAAAAGGAGCTATCAATCAACTCACAAAATATTTAGCCTGTGAATGGGCAAACGACAACATAAGGAGCAACGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTAGAACAAGTGTTGAGTAACAAAGCATACGTCGAAGAGGTATATTCTCGAACTCCACTTCGACGACTCGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTCGATGGAGGGATGTCTGTTAATGGCTTTGATCCAAGTTAA

Protein sequence

MLQSRQGQAGKTMEGGVVLKLESQTGETCNLLRPSPRTMFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIRNQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPS
Homology
BLAST of Carg12391 vs. NCBI nr
Match: KAG7036948.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.1e-300
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MLQSRQGQAGKTMEGGVVLKLESQTGETCNLLRPSPRTMFLALVSPSPFDSFSSSTFNPS 60
           MLQSRQGQAGKTMEGGVVLKLESQTGETCNLLRPSPRTMFLALVSPSPFDSFSSSTFNPS
Sbjct: 1   MLQSRQGQAGKTMEGGVVLKLESQTGETCNLLRPSPRTMFLALVSPSPFDSFSSSTFNPS 60

Query: 61  QPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIRNQAESDFKTKNGSTPGDHRWSL 120
           QPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIRNQAESDFKTKNGSTPGDHRWSL
Sbjct: 61  QPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIRNQAESDFKTKNGSTPGDHRWSL 120

Query: 121 HGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDV 180
           HGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDV
Sbjct: 121 HGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDV 180

Query: 181 SVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLAHCLE 240
           SVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLAHCLE
Sbjct: 181 SVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLAHCLE 240

Query: 241 FGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRCGISP 300
           FGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRCGISP
Sbjct: 241 FGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRCGISP 300

Query: 301 EDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRRKLVN 360
           EDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRRKLVN
Sbjct: 301 EDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRRKLVN 360

Query: 361 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSL 420
           NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSL
Sbjct: 361 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSL 420

Query: 421 KSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSR 480
           KSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSR
Sbjct: 421 KSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSR 480

Query: 481 TPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPS 527
           TPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPS
Sbjct: 481 TPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPS 526

BLAST of Carg12391 vs. NCBI nr
Match: KAG6607269.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.8 bits (1514), Expect = 9.3e-164
Identity = 332/488 (68.03%), Postives = 332/488 (68.03%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSP DSFSSSTFNPSQP LSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPSDSFSSSTFNPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE
Sbjct: 61  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY
Sbjct: 361 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. NCBI nr
Match: XP_022948630.1 (tropinone reductase homolog At5g06060-like [Cucurbita moschata])

HSP 1 Score: 584.3 bits (1505), Expect = 1.0e-162
Identity = 330/488 (67.62%), Postives = 331/488 (67.83%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSPFDS SSSTF+PSQP LSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPFDSLSSSTFSPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQA SDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE
Sbjct: 61  NQAASDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY
Sbjct: 361 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. NCBI nr
Match: XP_023523143.1 (tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo] >XP_023523926.1 tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581.3 bits (1497), Expect = 8.7e-162
Identity = 329/488 (67.42%), Postives = 329/488 (67.42%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSPFDS SSSTFNPSQP LSTFS S ISRSFKPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHLSTFSLSSISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE
Sbjct: 61  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY
Sbjct: 361 FLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. NCBI nr
Match: XP_022998606.1 (tropinone reductase homolog At5g06060-like [Cucurbita maxima])

HSP 1 Score: 573.5 bits (1477), Expect = 1.8e-159
Identity = 324/488 (66.39%), Postives = 327/488 (67.01%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSPFDS SSSTFNPSQP  STFSSSPISRS KPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQAESDFK KNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNE+E
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTF+GKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTN+ESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNIESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWY
Sbjct: 361 FLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 6.2e-86
Identity = 195/409 (47.68%), Postives = 215/409 (52.57%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           RWSL+G +ALVTGGTRGIGRAIVEEL G GA VHTC+RNE EL+ CL DW    F V+GS
Sbjct: 65  RWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGS 124

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R+QRE LM+TV+  F+GKL+I                                 
Sbjct: 125 VCDVSDRSQREALMETVSSVFEGKLHI--------------------------------- 184

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 185 ------------------------------------------------------------ 244

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 245 ------------------------------------------------------------ 304

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LVNNVG NIRKP  EFT  EFS LM TN ESVFHL QLA+P L+ S AGS+VF+SSVS 
Sbjct: 305 -LVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSG 319

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
           FVSLK+MSVQ +TKGAINQLT+ LACEWA DNIR NAVAPWYIKTSMVEQVLSNK Y+EE
Sbjct: 365 FVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEE 319

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 526
           VYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMS+NGF P
Sbjct: 425 VYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFP 319

BLAST of Carg12391 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.0e-80
Identity = 179/409 (43.77%), Postives = 208/409 (50.86%), Query Frame = 0

Query: 115 DHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVS 174
           D RWSL G TALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL DWK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 175 GSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSL 234
           GSVCD SVR QRE+L++  +  F GKLNI                               
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNI------------------------------- 123

Query: 235 LAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPN 294
                                                                       
Sbjct: 124 ------------------------------------------------------------ 183

Query: 295 RCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRP 354
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 355 RRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSV 414
              L+NNVG N+RKPT E++ EE++ +M TN+ES FHLSQ+AHP LKASG GSIVF+SSV
Sbjct: 244 ---LINNVGTNVRKPTVEYSSEEYAKIMSTNLESAFHLSQIAHPLLKASGVGSIVFISSV 258

Query: 415 SSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYV 474
           +  V L S S+ GATKGA+NQLT+ LACEWA+DNIR+N VAPWYIKTS+VE +L  K +V
Sbjct: 304 AGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCVAPWYIKTSLVETLLEKKEFV 258

Query: 475 EEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           E V SRTPL R+GEP EVSSLVAFLCLPASSYITGQ+I VDGG +VNGF
Sbjct: 364 EAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNGF 258

BLAST of Carg12391 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.1e-69
Identity = 160/409 (39.12%), Postives = 204/409 (49.88%), Query Frame = 0

Query: 115 DHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVS 174
           D RWSL G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR+W++  F+V+
Sbjct: 2   DKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVT 61

Query: 175 GSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSL 234
            S+CDVS+R QRE+LM+TV+  F GKLNI                               
Sbjct: 62  TSICDVSLREQREKLMETVSSLFQGKLNI------------------------------- 121

Query: 235 LAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPN 294
                                                                       
Sbjct: 122 ------------------------------------------------------------ 181

Query: 295 RCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRP 354
                                                                       
Sbjct: 182 ------------------------------------------------------------ 241

Query: 355 RRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSV 414
              LVNNVG  + KPTTE+T EEFSFLM TN++S FH+SQLAHP LKASG+GSIV MSS+
Sbjct: 242 ---LVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFHISQLAHPLLKASGSGSIVLMSSI 256

Query: 415 SSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYV 474
           +  V +   S+ GATKGA+NQL + LACEWA+DNIR+NA+ PW I T ++  +LS +   
Sbjct: 302 AGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIRTNAICPWLITTPLISDLLSVEEMK 256

Query: 475 EEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           +E   RTP+ R+GE +EVS LVAFLCLPA+SYITGQ+I VDGG++VNGF
Sbjct: 362 KEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQVICVDGGLTVNGF 256

BLAST of Carg12391 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.8e-67
Identity = 160/407 (39.31%), Postives = 202/407 (49.63%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R +RE+LM+TV+  F+GKLNI                                 
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNI--------------------------------- 132

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 133 ------------------------------------------------------------ 192

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 193 ------------------------------------------------------------ 252

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LVNNVG  I KPT + T E+FSF M TN+ES FHLSQLAHP LKASG+GSIV +SSVS 
Sbjct: 253 -LVNNVGTCIVKPTLQHTAEDFSFTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSG 265

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
            V +   S+ G +KGA+NQL + LACEWA+DNIR+N+V PW+I+T +V + LSN+ + +E
Sbjct: 313 VVHVNGASIYGVSKGAMNQLGRNLACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKE 265

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           V SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ I VDGG +VNGF
Sbjct: 373 VESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNGF 265

BLAST of Carg12391 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.1e-66
Identity = 158/407 (38.82%), Postives = 201/407 (49.39%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E +L++ LR+W+    +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R QRE+LM+TV+  F GKL+I                                 
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSI--------------------------------- 132

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 133 ------------------------------------------------------------ 192

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 193 ------------------------------------------------------------ 252

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LV NVG  + KPTTE T EEFSF++ TN+ES FH SQLAHP LKASG+G+IV MSSV+ 
Sbjct: 253 -LVPNVGIGVLKPTTECTAEEFSFIIATNLESTFHFSQLAHPLLKASGSGNIVLMSSVAG 265

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
            V+L + S+ GATKGA+NQL + LACEWA+DNIR+N+V PW+I T   +  L +K   E+
Sbjct: 313 VVNLGNTSIYGATKGAMNQLARNLACEWASDNIRANSVCPWFITTPSTKDFLGDKDVKEK 265

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           V S TPLRR+GE +EVSSLVAFLCLPA+SYITGQ I VDGG ++NGF
Sbjct: 373 VESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTINGF 265

BLAST of Carg12391 vs. ExPASy TrEMBL
Match: A0A6J1GAE7 (tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC111452252 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 5.0e-163
Identity = 330/488 (67.62%), Postives = 331/488 (67.83%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSPFDS SSSTF+PSQP LSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPFDSLSSSTFSPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQA SDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE
Sbjct: 61  NQAASDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY
Sbjct: 361 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. ExPASy TrEMBL
Match: A0A6J1KAL5 (tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111493193 PE=4 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 8.8e-160
Identity = 324/488 (66.39%), Postives = 327/488 (67.01%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           MFLALVSPSPFDS SSSTFNPSQP  STFSSSPISRS KPFWLPTSNSTHKLVKRLKSIR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQAESDFK KNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNE+E
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTF+GKLNI               
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTN+ESVFHLSQLAHP
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNIESVFHLSQLAHP 334

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
           FLKASG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWY
Sbjct: 361 FLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWY 334

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 334

Query: 519 SVNGFDPS 527
           SVNGFDPS
Sbjct: 481 SVNGFDPS 334

BLAST of Carg12391 vs. ExPASy TrEMBL
Match: A0A5D3BGS1 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001920 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 1.3e-134
Identity = 291/487 (59.75%), Postives = 298/487 (61.19%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           M LALVSPS F SFSSSTFN S    STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQ ESDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TFDGKLNI               
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLA+P
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYP 331

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
            LK+SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWY
Sbjct: 361 LLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWY 331

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 331

Query: 519 SVNGFDP 526
           SVNGFDP
Sbjct: 481 SVNGFDP 331

BLAST of Carg12391 vs. ExPASy TrEMBL
Match: A0A1S3C5I5 (tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 1.3e-134
Identity = 291/487 (59.75%), Postives = 298/487 (61.19%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTFNPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 98
           M LALVSPS F SFSSSTFN S    STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60

Query: 99  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 158
           NQ ESDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 159 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASF 218
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TFDGKLNI               
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNI--------------- 180

Query: 219 AGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLG 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 RGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRIL 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 NVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHP 398
                              LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLA+P
Sbjct: 301 -------------------LVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYP 331

Query: 399 FLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWY 458
            LK+SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWY
Sbjct: 361 LLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWY 331

Query: 459 IKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 518
           IKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM
Sbjct: 421 IKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGM 331

Query: 519 SVNGFDP 526
           SVNGFDP
Sbjct: 481 SVNGFDP 331

BLAST of Carg12391 vs. ExPASy TrEMBL
Match: A0A6J1CL23 (tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC111012129 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 4.7e-129
Identity = 282/488 (57.79%), Postives = 296/488 (60.66%), Query Frame = 0

Query: 39  MFLALVSPSPFDSFSSSTF-NPSQPRLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSI 98
           M LALVSP+ FDSFSS TF  P+ P LS  S   IS SF PF LP S ST K++ RLK I
Sbjct: 1   MLLALVSPTSFDSFSSFTFITPTNPPLSPIS---ISCSFNPFGLPPSYSTRKILHRLKPI 60

Query: 99  RNQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEN 158
            NQ + D K+ NG+TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRN +
Sbjct: 61  TNQTQPDLKSMNGTTPIDDRWSLRGATALVTGGTRGIGRAIVEELVGFGARVHTCSRNLS 120

Query: 159 ELKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPAS 218
           EL+QCLRDWK+LEF VSGSVCDVSVRAQREELM+TVA  FDGKLNI              
Sbjct: 121 ELQQCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAANFDGKLNI-------------- 180

Query: 219 FAGTPSRVYPLRGSTSLLAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLL 278
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 279 GRGFHTLTKKVSFSYPNRCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRI 338
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 339 LNVFLNSFRLVTHPQRPRRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAH 398
                               LVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLA+
Sbjct: 301 --------------------LVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAY 331

Query: 399 PFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPW 458
           P LKASG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPW
Sbjct: 361 PLLKASGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPW 331

Query: 459 YIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGG 518
           YIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGG
Sbjct: 421 YIKTSMVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGG 331

Query: 519 MSVNGFDP 526
           MS+NGFDP
Sbjct: 481 MSINGFDP 331

BLAST of Carg12391 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 4.4e-87
Identity = 195/409 (47.68%), Postives = 215/409 (52.57%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           RWSL+G +ALVTGGTRGIGRAIVEEL G GA VHTC+RNE EL+ CL DW    F V+GS
Sbjct: 65  RWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGS 124

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R+QRE LM+TV+  F+GKL+I                                 
Sbjct: 125 VCDVSDRSQREALMETVSSVFEGKLHI--------------------------------- 184

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 185 ------------------------------------------------------------ 244

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 245 ------------------------------------------------------------ 304

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LVNNVG NIRKP  EFT  EFS LM TN ESVFHL QLA+P L+ S AGS+VF+SSVS 
Sbjct: 305 -LVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSG 319

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
           FVSLK+MSVQ +TKGAINQLT+ LACEWA DNIR NAVAPWYIKTSMVEQVLSNK Y+EE
Sbjct: 365 FVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEE 319

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 526
           VYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMS+NGF P
Sbjct: 425 VYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFP 319

BLAST of Carg12391 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 7.2e-82
Identity = 179/409 (43.77%), Postives = 208/409 (50.86%), Query Frame = 0

Query: 115 DHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVS 174
           D RWSL G TALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL DWK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 175 GSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSL 234
           GSVCD SVR QRE+L++  +  F GKLNI                               
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNI------------------------------- 123

Query: 235 LAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPN 294
                                                                       
Sbjct: 124 ------------------------------------------------------------ 183

Query: 295 RCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRP 354
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 355 RRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSV 414
              L+NNVG N+RKPT E++ EE++ +M TN+ES FHLSQ+AHP LKASG GSIVF+SSV
Sbjct: 244 ---LINNVGTNVRKPTVEYSSEEYAKIMSTNLESAFHLSQIAHPLLKASGVGSIVFISSV 258

Query: 415 SSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYV 474
           +  V L S S+ GATKGA+NQLT+ LACEWA+DNIR+N VAPWYIKTS+VE +L  K +V
Sbjct: 304 AGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCVAPWYIKTSLVETLLEKKEFV 258

Query: 475 EEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           E V SRTPL R+GEP EVSSLVAFLCLPASSYITGQ+I VDGG +VNGF
Sbjct: 364 EAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNGF 258

BLAST of Carg12391 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.2e-70
Identity = 160/409 (39.12%), Postives = 204/409 (49.88%), Query Frame = 0

Query: 115 DHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVS 174
           D RWSL G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR+W++  F+V+
Sbjct: 2   DKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVT 61

Query: 175 GSVCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSL 234
            S+CDVS+R QRE+LM+TV+  F GKLNI                               
Sbjct: 62  TSICDVSLREQREKLMETVSSLFQGKLNI------------------------------- 121

Query: 235 LAHCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPN 294
                                                                       
Sbjct: 122 ------------------------------------------------------------ 181

Query: 295 RCGISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRP 354
                                                                       
Sbjct: 182 ------------------------------------------------------------ 241

Query: 355 RRKLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSV 414
              LVNNVG  + KPTTE+T EEFSFLM TN++S FH+SQLAHP LKASG+GSIV MSS+
Sbjct: 242 ---LVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFHISQLAHPLLKASGSGSIVLMSSI 256

Query: 415 SSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYV 474
           +  V +   S+ GATKGA+NQL + LACEWA+DNIR+NA+ PW I T ++  +LS +   
Sbjct: 302 AGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIRTNAICPWLITTPLISDLLSVEEMK 256

Query: 475 EEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           +E   RTP+ R+GE +EVS LVAFLCLPA+SYITGQ+I VDGG++VNGF
Sbjct: 362 KEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQVICVDGGLTVNGF 256

BLAST of Carg12391 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.7e-68
Identity = 160/407 (39.31%), Postives = 202/407 (49.63%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R +RE+LM+TV+  F+GKLNI                                 
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNI--------------------------------- 132

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 133 ------------------------------------------------------------ 192

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 193 ------------------------------------------------------------ 252

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LVNNVG  I KPT + T E+FSF M TN+ES FHLSQLAHP LKASG+GSIV +SSVS 
Sbjct: 253 -LVNNVGTCIVKPTLQHTAEDFSFTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSG 265

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
            V +   S+ G +KGA+NQL + LACEWA+DNIR+N+V PW+I+T +V + LSN+ + +E
Sbjct: 313 VVHVNGASIYGVSKGAMNQLGRNLACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKE 265

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           V SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ I VDGG +VNGF
Sbjct: 373 VESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNGF 265

BLAST of Carg12391 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 7.8e-68
Identity = 158/407 (38.82%), Postives = 201/407 (49.39%), Query Frame = 0

Query: 117 RWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGS 176
           +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E +L++ LR+W+    +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 177 VCDVSVRAQREELMKTVADTFDGKLNILVRPHNPPTSGPASFAGTPSRVYPLRGSTSLLA 236
           VCDVS R QRE+LM+TV+  F GKL+I                                 
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSI--------------------------------- 132

Query: 237 HCLEFGSYTICKSLSPPLLDIVFSRLFPFELPLKVFKTRLLGRGFHTLTKKVSFSYPNRC 296
                                                                       
Sbjct: 133 ------------------------------------------------------------ 192

Query: 297 GISPEDFLLEDEQLPKAKFHKQALETHTRTLDILSYPLSRILNVFLNSFRLVTHPQRPRR 356
                                                                       
Sbjct: 193 ------------------------------------------------------------ 252

Query: 357 KLVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSS 416
            LV NVG  + KPTTE T EEFSF++ TN+ES FH SQLAHP LKASG+G+IV MSSV+ 
Sbjct: 253 -LVPNVGIGVLKPTTECTAEEFSFIIATNLESTFHFSQLAHPLLKASGSGNIVLMSSVAG 265

Query: 417 FVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEE 476
            V+L + S+ GATKGA+NQL + LACEWA+DNIR+N+V PW+I T   +  L +K   E+
Sbjct: 313 VVNLGNTSIYGATKGAMNQLARNLACEWASDNIRANSVCPWFITTPSTKDFLGDKDVKEK 265

Query: 477 VYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGF 524
           V S TPLRR+GE +EVSSLVAFLCLPA+SYITGQ I VDGG ++NGF
Sbjct: 373 VESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTINGF 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036948.13.1e-300100.00Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
KAG6607269.19.3e-16468.03Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022948630.11.0e-16267.62tropinone reductase homolog At5g06060-like [Cucurbita moschata][more]
XP_023523143.18.7e-16267.42tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022998606.11.8e-15966.39tropinone reductase homolog At5g06060-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZW126.2e-8647.68Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9LHT01.0e-8043.77Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW133.1e-6939.12Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Q9ZW193.8e-6739.31Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=... [more]
Q9ZW201.1e-6638.82Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293... [more]
Match NameE-valueIdentityDescription
A0A6J1GAE75.0e-16367.62tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KAL58.8e-16066.39tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3BGS11.3e-13459.75Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C5I51.3e-13459.75tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE... [more]
A0A6J1CL234.7e-12957.79tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G29260.14.4e-8747.68NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G06060.17.2e-8243.77NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.22.2e-7039.12NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.12.7e-6839.31NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29370.17.8e-6838.82NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 114..226
e-value: 6.4E-22
score: 79.9
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 358..519
e-value: 5.9E-44
score: 150.4
NoneNo IPR availableGENE3D3.40.50.720coord: 356..524
e-value: 1.6E-51
score: 177.3
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 120..205
e-value: 16.0
score: 2.4
NoneNo IPR availablePANTHERPTHR42898:SF2TROPINONE REDUCTASE-LIKE PROTEIN, CHLOROPLASTICcoord: 358..525
coord: 96..205
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 124..205
e-value: 1.4E-15
score: 57.2
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 358..525
coord: 96..205
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 117..205
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 358..520

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12391-RACarg12391-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity