Carg12384 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12384 vs. NCBI nr
Match: KAG7036941.1 (Abscisic acid 8'-hydroxylase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120
           GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300
           LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA
Sbjct: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300

Query: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG 360
           VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG
Sbjct: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG 360

Query: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420
           YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420

Query: 421 LEILILLHHLITGYRSLFIIFSFFLHELSV 451
           LEILILLHHLITGYRSLFIIFSFFLHELSV
Sbjct: 421 LEILILLHHLITGYRSLFIIFSFFLHELSV 450

BLAST of Carg12384 vs. NCBI nr
Match: XP_022948432.1 (abscisic acid 8'-hydroxylase 2-like [Cucurbita moschata])

HSP 1 Score: 876.3 bits (2263), Expect = 1.1e-250
Identity = 432/435 (99.31%), Postives = 433/435 (99.54%), Query Frame = 0

Query: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPY GETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYTGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120
           GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQ+KRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQLKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300
           LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA
Sbjct: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300

Query: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG 360
           VKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEEVEFEG
Sbjct: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFEG 360

Query: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420
           YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420

Query: 421 LEILILLHHLITGYR 436
           LEILILLHHLITGYR
Sbjct: 421 LEILILLHHLITGYR 435

BLAST of Carg12384 vs. NCBI nr
Match: XP_023523016.1 (abscisic acid 8'-hydroxylase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 873.6 bits (2256), Expect = 7.2e-250
Identity = 430/435 (98.85%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120
           GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300
           LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLL A
Sbjct: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLGA 300

Query: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG 360
           VKKEQDAIFEE+VREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEEVEFEG
Sbjct: 301 VKKEQDAIFEEKVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFEG 360

Query: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420
           YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420

Query: 421 LEILILLHHLITGYR 436
           LEIL+LLHH ITGYR
Sbjct: 421 LEILVLLHHFITGYR 435

BLAST of Carg12384 vs. NCBI nr
Match: XP_022997685.1 (abscisic acid 8'-hydroxylase 2-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 873.6 bits (2256), Expect = 7.2e-250
Identity = 439/454 (96.70%), Postives = 443/454 (97.58%), Query Frame = 0

Query: 1   MLPLLLLFG---LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           MLPLLLLFG   LLLLLLLC QWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180
           HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLGGDGDGD--KVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300
           GGGLLAVLLGGDGDGD  KVEKNKL++SQIADNIIGVIFAAQDTTASVLTWILKYLHDDH
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEE 360
           HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEE
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420
           VEFEGYLIPRGWKVLPLFRTIHHSAHFF HPEKFDPSRFEV PRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVLPRPNTYMPFGNGVHSCPG 420

Query: 421 SEMAKLEILILLHHLITGYRSLFIIFSFFLHELS 450
           SEMAKLEIL+LLHHLI  YRSLFI+FSFFLHELS
Sbjct: 421 SEMAKLEILVLLHHLIIDYRSLFIVFSFFLHELS 454

BLAST of Carg12384 vs. NCBI nr
Match: KAG6607263.1 (Abscisic acid 8'-hydroxylase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 871.7 bits (2251), Expect = 2.7e-249
Identity = 433/436 (99.31%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MLPLLLLFG-LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKR 60
           MLPLLLLFG LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKR
Sbjct: 1   MLPLLLLFGLLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKR 60

Query: 61  YGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS 120
           YGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS
Sbjct: 61  YGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS 120

Query: 121 RLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQ 180
           RLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQ
Sbjct: 121 RLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQ 180

Query: 181 QDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG 240
           QDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG
Sbjct: 181 QDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG 240

Query: 241 GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 300
           GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE
Sbjct: 241 GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 300

Query: 301 AVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFE 360
           AVKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEEVEFE
Sbjct: 301 AVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFE 360

Query: 361 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 420
           GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEV PRPNTYMPFGNGVHSCPGSEMA
Sbjct: 361 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVSPRPNTYMPFGNGVHSCPGSEMA 420

Query: 421 KLEILILLHHLITGYR 436
           KLEILILLHHLITGYR
Sbjct: 421 KLEILILLHHLITGYR 436

BLAST of Carg12384 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 6.7e-166
Identity = 295/436 (67.66%), Postives = 361/436 (82.80%), Query Frame = 0

Query: 4   LLLLFGLLLLLLLCLQWRH--PNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 63
           + L   +++++LL   W H       LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YG
Sbjct: 23  ITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYG 82

Query: 64  DVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRL 123
           D+FKTHILGCPCVMISSPEAAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS L
Sbjct: 83  DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTL 142

Query: 124 KKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQ-INTLQQMKRFAFDVAMISAFGDQQ 183
           K+L+Q+SF+PSA++P+VS IE +VL+ L SW + + INTL+ MKR+AFDVA++SAFGD++
Sbjct: 143 KRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKE 202

Query: 184 D-LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG 243
           +   I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKR+ +    G
Sbjct: 203 EPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG 262

Query: 244 GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 303
           GLL VLLG        ++N LS+SQIADNIIGVIFAA DTTASVLTW+LKYLHD  +LL+
Sbjct: 263 GLLGVLLGAKDQ----KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQ 322

Query: 304 AVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFE 363
            V +EQ +I  +++++  R ++W+DTR+M LT+RVIQETLR ASVLSFTFREAV++VE++
Sbjct: 323 EVSREQFSI-RQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 382

Query: 364 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 423
           GYLIP+GWKVLPLFR IHHS+ FFP PEKFDPSRFEV P+P TYMPFGNGVHSCPGSE+A
Sbjct: 383 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 442

Query: 424 KLEILILLHHLITGYR 436
           KLE+LILLHHL T +R
Sbjct: 443 KLEMLILLHHLTTSFR 453

BLAST of Carg12384 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.1e-147
Identity = 258/428 (60.28%), Postives = 332/428 (77.57%), Query Frame = 0

Query: 10  LLLLLLLCL--QWRHPNHHL-LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKT 69
           L L  L CL  Q R  +  L LPPG+MGWPY+GETF+LY+Q+PN FF  +QKRYG VFKT
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKT 74

Query: 70  HILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQ 129
           H+LGCPCVMISSPEAA+ VLV+K+HLFKPT+P SKE+M+G QA+FFHQG YH++L+KL+ 
Sbjct: 75  HVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVL 134

Query: 130 ASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIER 189
            +F+P +I+  V  IE+I    L SW+ + INT Q+MK + F+VA++S FG  + L  E 
Sbjct: 135 RAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRED 194

Query: 190 IKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLL 249
           +K  Y  LEKGYNSMP++LPGT F K+MKARK LS+ L +++ +R+++      LL   +
Sbjct: 195 LKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM 254

Query: 250 GGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQD 309
           G        +K +L++ QIADNIIGVIFAA+DTTASV++WILKYL ++ ++LEAV +EQ 
Sbjct: 255 G--------DKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQM 314

Query: 310 AIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRG 369
           AI ++  +E G  LTW DT++M LTSRVIQETLR AS+LSFTFREAVE+VE+EGYLIP+G
Sbjct: 315 AIRKD--KEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 374

Query: 370 WKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILIL 429
           WKVLPLFR IHHSA  F +P KFDPSRFEV P+PNT+MPFGNG HSCPG+E+AKLE+ I+
Sbjct: 375 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 432

Query: 430 LHHLITGY 435
           +HHL T Y
Sbjct: 435 IHHLTTKY 432

BLAST of Carg12384 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.0e-145
Identity = 250/408 (61.27%), Postives = 320/408 (78.43%), Query Frame = 0

Query: 28  LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPEAARVVLV 87
           LPPG+MG+PY+GETF+LY+Q+PN FF+ +Q+RYG VFKTH+LGCPCVMISSPEAA+ VLV
Sbjct: 36  LPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 88  SKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQASFLPSAIKPSVSQIENIVLR 147
           +K+HLFKPT+P SKE+M+G QA+FFHQG YHS+L+KL+  +F+P AI+  V  IE+I   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQE 155

Query: 148 LLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPG 207
            L SWD +Q+NT Q+MK + F+VA+IS  G  +    E +K  Y  LEKGYNSMP++LPG
Sbjct: 156 SLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPG 215

Query: 208 TPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLLGGDGDGDKVEKNKLSNSQIAD 267
           T F KAMKARK L++ L  ++ KR+++      LL   +         +K  L++ QIAD
Sbjct: 216 TLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFM--------EDKAGLTDEQIAD 275

Query: 268 NIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQDAIFEERVREGGRGLTWDDTRR 327
           NIIGVIFAA+DTTASVLTWILKYL D+  +LEAV +EQ AI ++  ++ G  LTW+DT++
Sbjct: 276 NIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKD--KKEGESLTWEDTKK 335

Query: 328 MTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPE 387
           M LT RVIQETLR A++LSFTFREAVE+VE+EGYLIP+GWKVLPLFR IHH+A  F  P 
Sbjct: 336 MPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPG 395

Query: 388 KFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILILLHHLITGYR 436
           KFDPSRFEV P+PNT+MPFG+G+HSCPG+E+AKLEI +L+HHL T YR
Sbjct: 396 KFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYR 433

BLAST of Carg12384 vs. ExPASy Swiss-Prot
Match: K4CI52 (Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.8e-143
Identity = 250/409 (61.12%), Postives = 317/409 (77.51%), Query Frame = 0

Query: 28  LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPEAARVVLV 87
           LPPG++G PYIGETF+LY+QNPN FF+ + K+YG +FKT+ILGCPCVMISSPEAA+ VLV
Sbjct: 36  LPPGTLGLPYIGETFQLYSQNPNVFFASKVKKYGSIFKTYILGCPCVMISSPEAAKQVLV 95

Query: 88  SKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQASFLPSAIKPSVSQIENIVLR 147
           +KA+LFKPT+P SKE+M+G QA+FFHQG YH++L+KL+  +F P +I+  +  IE+I + 
Sbjct: 96  TKANLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLQAFKPDSIRNIIPDIESIAIT 155

Query: 148 LLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPG 207
            L S+    INT Q+MK + F+VA+IS FG  + L  E +K  Y  LEKGYNSMP++LPG
Sbjct: 156 SLESFQGRLINTYQEMKTYTFNVALISIFGKDEFLYREELKKCYYILEKGYNSMPINLPG 215

Query: 208 TPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLLGGDGDGDKVEKNKLSNSQIAD 267
           T F KAMKARK L++ + K+I  R+      G LL   +G        +K  L++ QIAD
Sbjct: 216 TLFNKAMKARKELAKIVAKIISTRREMKIDHGDLLGSFMG--------DKEGLTDEQIAD 275

Query: 268 NIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQDAIFEERVREG-GRGLTWDDTR 327
           N+IGVIFAA+DTTASVLTWILKYL ++  +L+AV +EQ+ I  ++   G  + L W DTR
Sbjct: 276 NVIGVIFAARDTTASVLTWILKYLGENPSVLQAVTEEQENIMRKKEVNGEEKVLNWQDTR 335

Query: 328 RMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRGWKVLPLFRTIHHSAHFFPHP 387
           +M +T+RVIQETLR AS+LSFTFREAVE+VEFEGYLIP+GWKVLPLFR IHHS   FP P
Sbjct: 336 QMPMTTRVIQETLRVASILSFTFREAVEDVEFEGYLIPKGWKVLPLFRNIHHSPDNFPEP 395

Query: 388 EKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILILLHHLITGYR 436
           EKFDPSRFEV P+PNT+MPFGNGVHSCPG+++AKLEILIL+HHL T YR
Sbjct: 396 EKFDPSRFEVSPKPNTFMPFGNGVHSCPGNDLAKLEILILVHHLTTKYR 436

BLAST of Carg12384 vs. ExPASy Swiss-Prot
Match: Q09J79 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.6e-143
Identity = 250/412 (60.68%), Postives = 319/412 (77.43%), Query Frame = 0

Query: 28  LPPGSMGWPYIGETFKLY-TQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPEAARVVL 87
           LPPGSMGWPY+GETF+LY ++NPN FF+ ++ +YG +FKTHILGCPCVM+SSPEAAR VL
Sbjct: 39  LPPGSMGWPYVGETFQLYSSKNPNVFFNKKRNKYGPIFKTHILGCPCVMVSSPEAARFVL 98

Query: 88  VSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQASFLPSAIKPSVSQIENIVL 147
           V++AHLFKPT+P SKE+M+GPQA+FF QG YH+ L++++  +F P +I+ SV  IE I L
Sbjct: 99  VTQAHLFKPTFPASKERMLGPQAIFFQQGDYHAHLRRIVSRAFSPESIRASVPAIEAIAL 158

Query: 148 RLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLP 207
           R L SWD   +NT Q+MK +A +VA++S FG+++   IE +K  Y  LEKGYNSMP++LP
Sbjct: 159 RSLHSWDGQFVNTFQEMKTYALNVALLSIFGEEEMRYIEELKQCYLTLEKGYNSMPVNLP 218

Query: 208 GTPFRKAMKARKLLSETLGKLI---EKRQRSNEYGGGLLAVLLGGDGDGDKVEKNKLSNS 267
           GT F KAMKARK L   +  +I    +RQR N+        LLG   DG    +  L+++
Sbjct: 219 GTLFHKAMKARKRLGAIVAHIISARRERQRGND--------LLGSFVDG----REALTDA 278

Query: 268 QIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQDAIFEERVREGGRGLTWD 327
           QIADN+IGVIFAA+DTTASVLTW++K+L D   +L+AV +EQ  I +E+    G  L+W 
Sbjct: 279 QIADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEK-EASGEPLSWA 338

Query: 328 DTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRGWKVLPLFRTIHHSAHFF 387
           DTRRM +TSRVIQET+R AS+LSFTFREAVE+VE++GYLIP+GWKVLPLFR IHH+   F
Sbjct: 339 DTRRMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKVLPLFRNIHHNPDHF 398

Query: 388 PHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILILLHHLITGYR 436
           P PEKFDPSRFEV P+PNT+MPFGNG HSCPG+E+AKLE+L+L HHL T YR
Sbjct: 399 PCPEKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMLVLFHHLATKYR 437

BLAST of Carg12384 vs. ExPASy TrEMBL
Match: A0A6J1G973 (abscisic acid 8'-hydroxylase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452117 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.4e-251
Identity = 432/435 (99.31%), Postives = 433/435 (99.54%), Query Frame = 0

Query: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPY GETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYTGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120
           GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQ+KRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQLKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300
           LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA
Sbjct: 241 LLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEA 300

Query: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEG 360
           VKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEEVEFEG
Sbjct: 301 VKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFEG 360

Query: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420
           YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAK 420

Query: 421 LEILILLHHLITGYR 436
           LEILILLHHLITGYR
Sbjct: 421 LEILILLHHLITGYR 435

BLAST of Carg12384 vs. ExPASy TrEMBL
Match: A0A6J1KAK6 (abscisic acid 8'-hydroxylase 2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492574 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 3.5e-250
Identity = 439/454 (96.70%), Postives = 443/454 (97.58%), Query Frame = 0

Query: 1   MLPLLLLFG---LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           MLPLLLLFG   LLLLLLLC QWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180
           HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLGGDGDGD--KVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300
           GGGLLAVLLGGDGDGD  KVEKNKL++SQIADNIIGVIFAAQDTTASVLTWILKYLHDDH
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEE 360
           HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEE
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420
           VEFEGYLIPRGWKVLPLFRTIHHSAHFF HPEKFDPSRFEV PRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVLPRPNTYMPFGNGVHSCPG 420

Query: 421 SEMAKLEILILLHHLITGYRSLFIIFSFFLHELS 450
           SEMAKLEIL+LLHHLI  YRSLFI+FSFFLHELS
Sbjct: 421 SEMAKLEILVLLHHLIIDYRSLFIVFSFFLHELS 454

BLAST of Carg12384 vs. ExPASy TrEMBL
Match: A0A6J1KC93 (abscisic acid 8'-hydroxylase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492574 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.7e-244
Identity = 427/440 (97.05%), Postives = 430/440 (97.73%), Query Frame = 0

Query: 1   MLPLLLLFG---LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           MLPLLLLFG   LLLLLLLC QWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180
           HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLGGDGDGD--KVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300
           GGGLLAVLLGGDGDGD  KVEKNKL++SQIADNIIGVIFAAQDTTASVLTWILKYLHDDH
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEE 360
           HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEE
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420
           VEFEGYLIPRGWKVLPLFRTIHHSAHFF HPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420

Query: 421 SEMAKLEILILLHHLITGYR 436
           SEMAKLEIL+LLHHLI  YR
Sbjct: 421 SEMAKLEILVLLHHLIIDYR 440

BLAST of Carg12384 vs. ExPASy TrEMBL
Match: A0A6J1K5S5 (abscisic acid 8'-hydroxylase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492574 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.4e-243
Identity = 426/440 (96.82%), Postives = 429/440 (97.50%), Query Frame = 0

Query: 1   MLPLLLLFG---LLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           MLPLLLLFG   LLLLLLLC QWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180
           HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLGGDGDGD--KVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300
           GGGLLAVLLGGDGDGD  KVEKNKL++SQIADNIIGVIFAAQDTTASVLTWILKYLHDDH
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEE 360
           HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRM LTSRVIQETLRTASVLSFTFREAVEE
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420
           VEFEGYLIPRGWKVLPLFRTIHHSAHFF HPEKFDPSRFEV PRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVLPRPNTYMPFGNGVHSCPG 420

Query: 421 SEMAKLEILILLHHLITGYR 436
           SEMAKLEIL+LLHHLI  YR
Sbjct: 421 SEMAKLEILVLLHHLIIDYR 440

BLAST of Carg12384 vs. ExPASy TrEMBL
Match: A0A1S3C5P5 (abscisic acid 8'-hydroxylase 2 OS=Cucumis melo OX=3656 GN=LOC103497144 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.6e-218
Identity = 380/434 (87.56%), Postives = 403/434 (92.86%), Query Frame = 0

Query: 4   LLLLFGL--LLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 63
           +LLL GL  L +LLLCLQW HP H LLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLGLFSLFILLLCLQWSHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 64  DVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRL 123
           DVFKTHILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS L
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSYL 120

Query: 124 KKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQD 183
           KKLIQ+SFLPSAIK S+SQIENIVL LLPSW+NSQINTLQQMKRFAFDVAMISAFGDQQD
Sbjct: 121 KKLIQSSFLPSAIKHSISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180

Query: 184 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGL 243
           LEIERIKHLYQCLEKGYNSMPLDLPGTPF KAMKARK+LSETLGK+IEKR+R+ E+GGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFHKAMKARKVLSETLGKMIEKRRRNKEHGGGL 240

Query: 244 LAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAV 303
           LAVLL G G G++ EK KLS+SQIADNIIGVIFAAQDTTASVLTWILKYLHD+HHLLEAV
Sbjct: 241 LAVLLSG-GGGEEEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLEAV 300

Query: 304 KKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGY 363
           KKEQDAI+E ++ EG RGL+WDDTRRM  TSRVI ETLR ASV+SFTFREAVEEVEFEGY
Sbjct: 301 KKEQDAIYERKLCEGKRGLSWDDTRRMPFTSRVILETLRRASVVSFTFREAVEEVEFEGY 360

Query: 364 LIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKL 423
           LIP+GWKVLPLFRTIHHS  FFPHP  FDPSRFE PPRPNTYMPFGNGVHSCPGSEMAKL
Sbjct: 361 LIPKGWKVLPLFRTIHHSPDFFPHPHNFDPSRFEEPPRPNTYMPFGNGVHSCPGSEMAKL 420

Query: 424 EILILLHHLITGYR 436
           E+L+LLHHL T YR
Sbjct: 421 EMLVLLHHLTTTYR 433

BLAST of Carg12384 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.8e-167
Identity = 295/436 (67.66%), Postives = 361/436 (82.80%), Query Frame = 0

Query: 4   LLLLFGLLLLLLLCLQWRH--PNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 63
           + L   +++++LL   W H       LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YG
Sbjct: 23  ITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYG 82

Query: 64  DVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRL 123
           D+FKTHILGCPCVMISSPEAAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS L
Sbjct: 83  DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTL 142

Query: 124 KKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQ-INTLQQMKRFAFDVAMISAFGDQQ 183
           K+L+Q+SF+PSA++P+VS IE +VL+ L SW + + INTL+ MKR+AFDVA++SAFGD++
Sbjct: 143 KRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKE 202

Query: 184 D-LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG 243
           +   I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKR+ +    G
Sbjct: 203 EPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG 262

Query: 244 GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 303
           GLL VLLG        ++N LS+SQIADNIIGVIFAA DTTASVLTW+LKYLHD  +LL+
Sbjct: 263 GLLGVLLGAKDQ----KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQ 322

Query: 304 AVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFE 363
            V +EQ +I  +++++  R ++W+DTR+M LT+RVIQETLR ASVLSFTFREAV++VE++
Sbjct: 323 EVSREQFSI-RQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 382

Query: 364 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 423
           GYLIP+GWKVLPLFR IHHS+ FFP PEKFDPSRFEV P+P TYMPFGNGVHSCPGSE+A
Sbjct: 383 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 442

Query: 424 KLEILILLHHLITGYR 436
           KLE+LILLHHL T +R
Sbjct: 443 KLEMLILLHHLTTSFR 453

BLAST of Carg12384 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.8e-167
Identity = 295/436 (67.66%), Postives = 361/436 (82.80%), Query Frame = 0

Query: 4   LLLLFGLLLLLLLCLQWRH--PNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 63
           + L   +++++LL   W H       LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YG
Sbjct: 23  ITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYG 82

Query: 64  DVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRL 123
           D+FKTHILGCPCVMISSPEAAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS L
Sbjct: 83  DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTL 142

Query: 124 KKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQ-INTLQQMKRFAFDVAMISAFGDQQ 183
           K+L+Q+SF+PSA++P+VS IE +VL+ L SW + + INTL+ MKR+AFDVA++SAFGD++
Sbjct: 143 KRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKE 202

Query: 184 D-LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGG 243
           +   I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKR+ +    G
Sbjct: 203 EPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG 262

Query: 244 GLLAVLLGGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 303
           GLL VLLG        ++N LS+SQIADNIIGVIFAA DTTASVLTW+LKYLHD  +LL+
Sbjct: 263 GLLGVLLGAKDQ----KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQ 322

Query: 304 AVKKEQDAIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFE 363
            V +EQ +I  +++++  R ++W+DTR+M LT+RVIQETLR ASVLSFTFREAV++VE++
Sbjct: 323 EVSREQFSI-RQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 382

Query: 364 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 423
           GYLIP+GWKVLPLFR IHHS+ FFP PEKFDPSRFEV P+P TYMPFGNGVHSCPGSE+A
Sbjct: 383 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 442

Query: 424 KLEILILLHHLITGYR 436
           KLE+LILLHHL T +R
Sbjct: 443 KLEMLILLHHLTTSFR 453

BLAST of Carg12384 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 524.6 bits (1350), Expect = 7.7e-149
Identity = 259/429 (60.37%), Postives = 333/429 (77.62%), Query Frame = 0

Query: 10  LLLLLLLCL--QWRHPNHHL-LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKT 69
           L L  L CL  Q R  +  L LPPG+MGWPY+GETF+LY+Q+PN FF  +QKRYG VFKT
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKT 74

Query: 70  HILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQ 129
           H+LGCPCVMISSPEAA+ VLV+K+HLFKPT+P SKE+M+G QA+FFHQG YH++L+KL+ 
Sbjct: 75  HVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVL 134

Query: 130 ASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIER 189
            +F+P +I+  V  IE+I    L SW+ + INT Q+MK + F+VA++S FG  + L  E 
Sbjct: 135 RAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRED 194

Query: 190 IKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLL 249
           +K  Y  LEKGYNSMP++LPGT F K+MKARK LS+ L +++ +R+++      LL   +
Sbjct: 195 LKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM 254

Query: 250 GGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQD 309
           G        +K +L++ QIADNIIGVIFAA+DTTASV++WILKYL ++ ++LEAV +EQ 
Sbjct: 255 G--------DKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQM 314

Query: 310 AIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRG 369
           AI ++  +E G  LTW DT++M LTSRVIQETLR AS+LSFTFREAVE+VE+EGYLIP+G
Sbjct: 315 AIRKD--KEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 374

Query: 370 WKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILIL 429
           WKVLPLFR IHHSA  F +P KFDPSRFEV P+PNT+MPFGNG HSCPG+E+AKLE+ I+
Sbjct: 375 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 433

Query: 430 LHHLITGYR 436
           +HHL T YR
Sbjct: 435 IHHLTTKYR 433

BLAST of Carg12384 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 522.7 bits (1345), Expect = 2.9e-148
Identity = 258/428 (60.28%), Postives = 332/428 (77.57%), Query Frame = 0

Query: 10  LLLLLLLCL--QWRHPNHHL-LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKT 69
           L L  L CL  Q R  +  L LPPG+MGWPY+GETF+LY+Q+PN FF  +QKRYG VFKT
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKT 74

Query: 70  HILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQ 129
           H+LGCPCVMISSPEAA+ VLV+K+HLFKPT+P SKE+M+G QA+FFHQG YH++L+KL+ 
Sbjct: 75  HVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVL 134

Query: 130 ASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIER 189
            +F+P +I+  V  IE+I    L SW+ + INT Q+MK + F+VA++S FG  + L  E 
Sbjct: 135 RAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRED 194

Query: 190 IKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLL 249
           +K  Y  LEKGYNSMP++LPGT F K+MKARK LS+ L +++ +R+++      LL   +
Sbjct: 195 LKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM 254

Query: 250 GGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQD 309
           G        +K +L++ QIADNIIGVIFAA+DTTASV++WILKYL ++ ++LEAV +EQ 
Sbjct: 255 G--------DKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQM 314

Query: 310 AIFEERVREGGRGLTWDDTRRMTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRG 369
           AI ++  +E G  LTW DT++M LTSRVIQETLR AS+LSFTFREAVE+VE+EGYLIP+G
Sbjct: 315 AIRKD--KEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKG 374

Query: 370 WKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILIL 429
           WKVLPLFR IHHSA  F +P KFDPSRFEV P+PNT+MPFGNG HSCPG+E+AKLE+ I+
Sbjct: 375 WKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIM 432

Query: 430 LHHLITGY 435
           +HHL T Y
Sbjct: 435 IHHLTTKY 432

BLAST of Carg12384 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 518.1 bits (1333), Expect = 7.2e-147
Identity = 250/408 (61.27%), Postives = 320/408 (78.43%), Query Frame = 0

Query: 28  LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPEAARVVLV 87
           LPPG+MG+PY+GETF+LY+Q+PN FF+ +Q+RYG VFKTH+LGCPCVMISSPEAA+ VLV
Sbjct: 36  LPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 88  SKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSRLKKLIQASFLPSAIKPSVSQIENIVLR 147
           +K+HLFKPT+P SKE+M+G QA+FFHQG YHS+L+KL+  +F+P AI+  V  IE+I   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQE 155

Query: 148 LLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPG 207
            L SWD +Q+NT Q+MK + F+VA+IS  G  +    E +K  Y  LEKGYNSMP++LPG
Sbjct: 156 SLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPG 215

Query: 208 TPFRKAMKARKLLSETLGKLIEKRQRSNEYGGGLLAVLLGGDGDGDKVEKNKLSNSQIAD 267
           T F KAMKARK L++ L  ++ KR+++      LL   +         +K  L++ QIAD
Sbjct: 216 TLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFM--------EDKAGLTDEQIAD 275

Query: 268 NIIGVIFAAQDTTASVLTWILKYLHDDHHLLEAVKKEQDAIFEERVREGGRGLTWDDTRR 327
           NIIGVIFAA+DTTASVLTWILKYL D+  +LEAV +EQ AI ++  ++ G  LTW+DT++
Sbjct: 276 NIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKD--KKEGESLTWEDTKK 335

Query: 328 MTLTSRVIQETLRTASVLSFTFREAVEEVEFEGYLIPRGWKVLPLFRTIHHSAHFFPHPE 387
           M LT RVIQETLR A++LSFTFREAVE+VE+EGYLIP+GWKVLPLFR IHH+A  F  P 
Sbjct: 336 MPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPG 395

Query: 388 KFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMAKLEILILLHHLITGYR 436
           KFDPSRFEV P+PNT+MPFG+G+HSCPG+E+AKLEI +L+HHL T YR
Sbjct: 396 KFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYR 433