Carg12365 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12365
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cyclin-dependent protein kinase inhibitor SMR3-like
Location	Carg_Chr01: 3218750 .. 3219174 (-)
RNA-Seq Expression	Carg12365
Synteny	Carg12365

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTCTTCAATTCCACTATCTTACTCTCTGAAAATGATGTAAGCGCCATGGAATTCACATTTCTTCTCACATACTTGACTTCCAAACATCAAGACCACGACCTCGACCACGAAAGCAAATCTGTTCAAGACGATGACAATGGGTTCAAAACTCCGACTTCTTTGGAGCATAAAATCCCGGCAAGTCTCCGGTGTCCGATGGCGCCGAGGAAACCGAAATCTCGACCGATGATGAAGCGGAAGGCAGTTGAGCGACAGCGTTTGTTTCTGGATCTGTCGAGCGAAATTCAATCTTTGTTTCCAGCTGATTTTCTTGTAGGGCTTGGTGGTAAGATGAAGAGGGTTAGACAAGAATCAAAAAAATAACTACAAATGTATTACTTGCTTTCTTTTGGTTTTAGCTCTTCAAATTGATCTTGTAG

mRNA sequence

ATGGCCCTCTTCAATTCCACTATCTTACTCTCTGAAAATGATGTAAGCGCCATGGAATTCACATTTCTTCTCACATACTTGACTTCCAAACATCAAGACCACGACCTCGACCACGAAAGCAAATCTGTTCAAGACGATGACAATGGGTTCAAAACTCCGACTTCTTTGGAGCATAAAATCCCGGCAAGTCTCCGGTGTCCGATGGCGCCGAGGAAACCGAAATCTCGACCGATGATGAAGCGGAAGGCAGTTGAGCGACAGCGTTTGTTTCTGGATCTGTCGAGCGAAATTCAATCTTTGTTTCCAGCTGATTTTCTTGTAGGGCTTGGTGGTAAGATGAAGAGGCTCTTCAAATTGATCTTGTAG

Coding sequence (CDS)

ATGGCCCTCTTCAATTCCACTATCTTACTCTCTGAAAATGATGTAAGCGCCATGGAATTCACATTTCTTCTCACATACTTGACTTCCAAACATCAAGACCACGACCTCGACCACGAAAGCAAATCTGTTCAAGACGATGACAATGGGTTCAAAACTCCGACTTCTTTGGAGCATAAAATCCCGGCAAGTCTCCGGTGTCCGATGGCGCCGAGGAAACCGAAATCTCGACCGATGATGAAGCGGAAGGCAGTTGAGCGACAGCGTTTGTTTCTGGATCTGTCGAGCGAAATTCAATCTTTGTTTCCAGCTGATTTTCTTGTAGGGCTTGGTGGTAAGATGAAGAGGCTCTTCAAATTGATCTTGTAG

Protein sequence

MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVQDDDNGFKTPTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKRLFKLIL

Homology

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BLAST of Carg12365 vs. NCBI nr
Match: KAG7036922.1 (hypothetical protein SDJN02_00542, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 234.6 bits (597), Expect = 4.6e-58
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVQDDDNGFKTPTSLEHKI 60
           MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVQDDDNGFKTPTSLEHKI
Sbjct: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVQDDDNGFKTPTSLEHKI 60

Query: 61  PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKRLFKLI 120
           PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKRLFKLI
Sbjct: 61  PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKRLFKLI 120

Query: 121 L 122
           L
Sbjct: 121 L 121

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BLAST of Carg12365 vs. NCBI nr
Match: KAG6607237.1 (hypothetical protein SDJN03_00579, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 216.1 bits (549), Expect = 1.7e-52
Identity = 110/111 (99.10%), Postives = 111/111 (100.00%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVQDDDNGFKTPTSLEHKI 60
           MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSV+DDDNGFKTPTSLEHKI
Sbjct: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKSVEDDDNGFKTPTSLEHKI 60

Query: 61  PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGG 112
           PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGG
Sbjct: 61  PASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGG 111

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BLAST of Carg12365 vs. NCBI nr
Match: XP_011658114.1 (cyclin-dependent protein kinase inhibitor SMR3 [Cucumis sativus] >KGN65712.1 hypothetical protein Csa_019570 [Cucumis sativus])

HSP 1 Score: 167.5 bits (423), Expect = 6.9e-38
Identity = 89/124 (71.77%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHE--------SKSVQDDDNGFKT 60
           M LFNS+ILLSENDV++MEFTFLL Y+ SK  D + D +        S SV+DDDNGFKT
Sbjct: 1   MGLFNSSILLSENDVNSMEFTFLLRYVNSKEDDQEEDGQEETLNGGRSNSVEDDDNGFKT 60

Query: 61  PTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGK 117
           PTSLEHKIP SL+CPMAPRKPK R + KRKAVER RL LDLSSEIQSLFPA+FLVGLGGK
Sbjct: 61  PTSLEHKIPTSLQCPMAPRKPKPRRLTKRKAVERHRLLLDLSSEIQSLFPAEFLVGLGGK 120

[Close]

BLAST of Carg12365 vs. NCBI nr
Match: XP_016902107.1 (PREDICTED: cyclin-dependent protein kinase inhibitor SMR3-like [Cucumis melo] >KAA0067708.1 cyclin-dependent protein kinase inhibitor SMR3-like [Cucumis melo var. makuwa] >TYK21932.1 cyclin-dependent protein kinase inhibitor SMR3-like [Cucumis melo var. makuwa])

HSP 1 Score: 163.3 bits (412), Expect = 1.3e-36
Identity = 85/121 (70.25%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 4   FNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKS--------VQDDDNGFKTPTS 63
           FNS+ILLSENDV++MEFTFLL Y+ SK  D + D + ++        VQDDDNGFKTPTS
Sbjct: 6   FNSSILLSENDVNSMEFTFLLRYVNSKEDDQEEDRQEETLNGRSNSVVQDDDNGFKTPTS 65

Query: 64  LEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKR 117
           LEHKIP S++CPMAPRKPK R + KRKAVER RL LDLSSEIQSLFPA+FLVGLGGK+KR
Sbjct: 66  LEHKIPTSIQCPMAPRKPKPRRLTKRKAVERHRLLLDLSSEIQSLFPAEFLVGLGGKIKR 125

[Close]

BLAST of Carg12365 vs. NCBI nr
Match: KAE8039043.1 (hypothetical protein FH972_011491 [Carpinus fangiana])

HSP 1 Score: 99.0 bits (245), Expect = 3.0e-17
Identity = 62/145 (42.76%), Postives = 85/145 (58.62%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDH---------------------- 60
           M + +S + LSE D++AMEF FL+       +DH                          
Sbjct: 11  MGVSDSQMFLSEKDLNAMEFNFLVPPTLEFQEDHGKPEVVKQEEEEEEENKKKKKINIPS 70

Query: 61  ----ESKSVQDDDNGFKTPTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLS 119
               E + V+DD++GFKTPTSL+HKIPA L+CP APRKPK+ P+ KRKA  RQR+ LDLS
Sbjct: 71  VTVGEFRDVEDDEDGFKTPTSLDHKIPAILQCPPAPRKPKTIPLTKRKAA-RQRILLDLS 130

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BLAST of Carg12365 vs. ExPASy Swiss-Prot
Match: Q9LTJ2 (Cyclin-dependent protein kinase inhibitor SMR14 OS=Arabidopsis thaliana OX=3702 GN=SMR14 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 35/99 (35.35%), Postives = 52/99 (52.53%), Query Frame = 0


Query: 31  HQDHDLDHESKSVQDDDNGFKTPTSLEH----KIPASLR-------C-PMAPRKPKSRPM 90
           HQ    D E +++QD   G +     +     KIP + R       C P  PRKPK+ P 
Sbjct: 53  HQHRQQDQELENLQDGSRGKRKWECKDEESPVKIPETPRNSNSVCYCPPRPPRKPKAIPS 112

Query: 91  MKRKA--VERQRLFLDLSSEIQSLFPADFLVGLGGKMKR 116
           MKR+   V+R  +FLD++ E++S+FP   L   G K+K+
Sbjct: 113 MKRRGMWVKRSVVFLDVAREVESMFPPSVLQDFGKKIKK 151

[Close]

BLAST of Carg12365 vs. ExPASy TrEMBL
Match: A0A0A0LY05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G506960 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.3e-38
Identity = 89/124 (71.77%), Postives = 101/124 (81.45%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHE--------SKSVQDDDNGFKT 60
           M LFNS+ILLSENDV++MEFTFLL Y+ SK  D + D +        S SV+DDDNGFKT
Sbjct: 1   MGLFNSSILLSENDVNSMEFTFLLRYVNSKEDDQEEDGQEETLNGGRSNSVEDDDNGFKT 60

Query: 61  PTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGK 117
           PTSLEHKIP SL+CPMAPRKPK R + KRKAVER RL LDLSSEIQSLFPA+FLVGLGGK
Sbjct: 61  PTSLEHKIPTSLQCPMAPRKPKPRRLTKRKAVERHRLLLDLSSEIQSLFPAEFLVGLGGK 120

[Close]

BLAST of Carg12365 vs. ExPASy TrEMBL
Match: A0A5A7VIY0 (Cyclin-dependent protein kinase inhibitor SMR3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold710G00040 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.3e-37
Identity = 85/121 (70.25%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 4   FNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKS--------VQDDDNGFKTPTS 63
           FNS+ILLSENDV++MEFTFLL Y+ SK  D + D + ++        VQDDDNGFKTPTS
Sbjct: 6   FNSSILLSENDVNSMEFTFLLRYVNSKEDDQEEDRQEETLNGRSNSVVQDDDNGFKTPTS 65

Query: 64  LEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKR 117
           LEHKIP S++CPMAPRKPK R + KRKAVER RL LDLSSEIQSLFPA+FLVGLGGK+KR
Sbjct: 66  LEHKIPTSIQCPMAPRKPKPRRLTKRKAVERHRLLLDLSSEIQSLFPAEFLVGLGGKIKR 125

[Close]

BLAST of Carg12365 vs. ExPASy TrEMBL
Match: A0A1S4E1K5 (cyclin-dependent protein kinase inhibitor SMR3-like OS=Cucumis melo OX=3656 GN=LOC107991540 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.3e-37
Identity = 85/121 (70.25%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 4   FNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDHESKS--------VQDDDNGFKTPTS 63
           FNS+ILLSENDV++MEFTFLL Y+ SK  D + D + ++        VQDDDNGFKTPTS
Sbjct: 6   FNSSILLSENDVNSMEFTFLLRYVNSKEDDQEEDRQEETLNGRSNSVVQDDDNGFKTPTS 65

Query: 64  LEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQSLFPADFLVGLGGKMKR 117
           LEHKIP S++CPMAPRKPK R + KRKAVER RL LDLSSEIQSLFPA+FLVGLGGK+KR
Sbjct: 66  LEHKIPTSIQCPMAPRKPKPRRLTKRKAVERHRLLLDLSSEIQSLFPAEFLVGLGGKIKR 125

[Close]

BLAST of Carg12365 vs. ExPASy TrEMBL
Match: A0A660KRH4 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_011491 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.5e-17
Identity = 62/145 (42.76%), Postives = 85/145 (58.62%), Query Frame = 0


Query: 1   MALFNSTILLSENDVSAMEFTFLLTYLTSKHQDHDLDH---------------------- 60
           M + +S + LSE D++AMEF FL+       +DH                          
Sbjct: 11  MGVSDSQMFLSEKDLNAMEFNFLVPPTLEFQEDHGKPEVVKQEEEEEEENKKKKKINIPS 70

Query: 61  ----ESKSVQDDDNGFKTPTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLS 119
               E + V+DD++GFKTPTSL+HKIPA L+CP APRKPK+ P+ KRKA  RQR+ LDLS
Sbjct: 71  VTVGEFRDVEDDEDGFKTPTSLDHKIPAILQCPPAPRKPKTIPLTKRKAA-RQRILLDLS 130

[Close]

BLAST of Carg12365 vs. ExPASy TrEMBL
Match: A0A7N2KPB5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.4e-15
Identity = 49/78 (62.82%), Postives = 60/78 (76.92%), Query Frame = 0


Query: 39  ESKSVQDDDNGFKTPTSLEHKIPASLRCPMAPRKPKSRPMMKRKAVERQRLFLDLSSEIQ 98
           E + V+DDD+GF+TPTSL+HKIP SL+CP APRKPK  P  KRKA  R+R+ LDLS+EI 
Sbjct: 93  ELRDVEDDDDGFRTPTSLDHKIPDSLQCPPAPRKPKPIPSTKRKA-GRRRIMLDLSNEID 152

Query: 99  SLFPADFLVGLGGKMKRL 117
           SLFP   LV L  K+KR+
Sbjct: 153 SLFPPALLVDLRVKIKRI 169

[Close]

BLAST of Carg12365 vs. TAIR 10
Match: AT5G59360.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 9.7e-06
Identity = 35/99 (35.35%), Postives = 52/99 (52.53%), Query Frame = 0


Query: 31  HQDHDLDHESKSVQDDDNGFKTPTSLEH----KIPASLR-------C-PMAPRKPKSRPM 90
           HQ    D E +++QD   G +     +     KIP + R       C P  PRKPK+ P 
Sbjct: 53  HQHRQQDQELENLQDGSRGKRKWECKDEESPVKIPETPRNSNSVCYCPPRPPRKPKAIPS 112

Query: 91  MKRKA--VERQRLFLDLSSEIQSLFPADFLVGLGGKMKR 116
           MKR+   V+R  +FLD++ E++S+FP   L   G K+K+
Sbjct: 113 MKRRGMWVKRSVVFLDVAREVESMFPPSVLQDFGKKIKK 151

[Close]

BLAST of Carg12365 vs. TAIR 10
Match: AT5G02420.1 (unknown protein; Has 90 Blast hits to 90 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 90; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 44.3 bits (103), Expect = 8.2e-05
Identity = 33/74 (44.59%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 35  DLDHESKSVQDDDNGFKTPTSLEHKIP--ASLRCPMAPRKPKSRPM--MKRKAVE---RQ 94
           D DHE  +V +++ G KTPTS +HKIP      CP APRKPK       KRK        
Sbjct: 30  DSDHEDPTVNEEE-GCKTPTSSDHKIPEVKYTLCPPAPRKPKPNRSSGTKRKLTPVNVVN 89

Query: 95  RLFLDLSSEIQSLF 102
           R+ +DLS EI+  F
Sbjct: 90  RIPIDLSREIEMFF 102

The following BLAST results are available for this feature:

BLAST of Carg12365 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036922.1	4.6e-58	100.00	hypothetical protein SDJN02_00542, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6607237.1	1.7e-52	99.10	hypothetical protein SDJN03_00579, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_011658114.1	6.9e-38	71.77	cyclin-dependent protein kinase inhibitor SMR3 [Cucumis sativus] >KGN65712.1 hyp...	[more]
XP_016902107.1	1.3e-36	70.25	PREDICTED: cyclin-dependent protein kinase inhibitor SMR3-like [Cucumis melo] >K...	[more]
KAE8039043.1	3.0e-17	42.76	hypothetical protein FH972_011491 [Carpinus fangiana]	[more]

BLAST of Carg12365 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTJ2	1.4e-04	35.35	Cyclin-dependent protein kinase inhibitor SMR14 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg12365 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY05	3.3e-38	71.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G506960 PE=4 SV=1	[more]
A0A5A7VIY0	6.3e-37	70.25	Cyclin-dependent protein kinase inhibitor SMR3-like OS=Cucumis melo var. makuwa ...	[more]
A0A1S4E1K5	6.3e-37	70.25	cyclin-dependent protein kinase inhibitor SMR3-like OS=Cucumis melo OX=3656 GN=L...	[more]
A0A660KRH4	1.5e-17	42.76	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_011491 PE=4 SV=1	[more]
A0A7N2KPB5	1.4e-15	62.82	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Carg12365 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59360.1	9.7e-06	35.35	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]
AT5G02420.1	8.2e-05	44.59	unknown protein; Has 90 Blast hits to 90 proteins in 10 species: Archae - 0; Bac...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..80
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..55
None	No IPR available	PANTHER	PTHR33142:SF66	CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR2-LIKE	coord: 33..115
IPR040389	Cyclin-dependent protein kinase inhibitor SMR	PANTHER	PTHR33142	CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR13	coord: 33..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12365-RA	Carg12365-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045839	negative regulation of mitotic nuclear division
biological_process	GO:0032875	regulation of DNA endoreduplication
cellular_component	GO:0005634	nucleus