Carg12353 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12353 vs. NCBI nr
Match: KAG7036910.1 (E3 UFM1-protein ligase 1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1539.2 bits (3984), Expect = 0.0e+00
Identity = 817/817 (100.00%), Postives = 817/817 (100.00%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDELYGKALDLFEDDQSISVILHRHLLRTTA 600
           NNSWRERRKALFSENAEKMKRLLDNTQQKLDELYGKALDLFEDDQSISVILHRHLLRTTA
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDELYGKALDLFEDDQSISVILHRHLLRTTA 600

Query: 601 APIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKAVTVAEA 660
           APIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKAVTVAEA
Sbjct: 601 APIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKAVTVAEA 660

Query: 661 LEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPIALLPKV 720
           LEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPIALLPKV
Sbjct: 661 LEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPIALLPKV 720

Query: 721 VSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLISAAAGDE 780
           VSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLISAAAGDE
Sbjct: 721 VSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLISAAAGDE 780

Query: 781 DDCSSDRILAKREFLESQIPALKGLVSSLGSNISSNS 818
           DDCSSDRILAKREFLESQIPALKGLVSSLGSNISSNS
Sbjct: 781 DDCSSDRILAKREFLESQIPALKGLVSSLGSNISSNS 817

BLAST of Carg12353 vs. NCBI nr
Match: XP_022948887.1 (E3 UFM1-protein ligase 1 homolog isoform X2 [Cucurbita moschata] >XP_022948889.1 E3 UFM1-protein ligase 1 homolog isoform X2 [Cucurbita moschata])

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 795/814 (97.67%), Postives = 800/814 (98.28%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSS KFQTDPSMFSESI T
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLD------ELYGKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFSENAEKMKRLLDNTQQKLD      +LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTA+PIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601 LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLVSS 809
           AAAGDEDDCSSDRIL KREFLESQIPALKGLV S
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLVFS 814

BLAST of Carg12353 vs. NCBI nr
Match: XP_022948885.1 (E3 UFM1-protein ligase 1 homolog isoform X1 [Cucurbita moschata] >XP_022948886.1 E3 UFM1-protein ligase 1 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 794/812 (97.78%), Postives = 799/812 (98.40%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSS KFQTDPSMFSESI T
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLD------ELYGKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFSENAEKMKRLLDNTQQKLD      +LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTA+PIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601 LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           AAAGDEDDCSSDRIL KREFLESQIPALKGLV
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLV 812

BLAST of Carg12353 vs. NCBI nr
Match: XP_023522814.1 (E3 UFM1-protein ligase 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 790/812 (97.29%), Postives = 796/812 (98.03%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VS+MVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSSMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYA MEKEMETINVPGSSIGTFSGDSLSS KFQTDPSMFSESI  
Sbjct: 361 IFGDSFIFSNIFIKDLYASMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIEM 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGE MEK+SKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGEIMEKRSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHRH 600
           NNSWRERRK LFSENAEKMKRLLDNTQQKLDE      LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKTLFSENAEKMKRLLDNTQQKLDESFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLS+KA
Sbjct: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSSKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           AAAGDEDDCSSDRIL KREFLESQIPALKGLV
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLV 812

BLAST of Carg12353 vs. NCBI nr
Match: XP_022997980.1 (E3 UFM1-protein ligase 1 homolog [Cucurbita maxima] >XP_022997981.1 E3 UFM1-protein ligase 1 homolog [Cucurbita maxima])

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 787/812 (96.92%), Postives = 792/812 (97.54%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDD RLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDSRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIW SLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWISLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFE QDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEAQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEME INVPGSSIGTFSGDSLSS KFQTDPSMFSESI  
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMEAINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIEM 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNS RTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSCRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHRH 600
           NNSWRER+KALFSENAEKMKRLLDNTQQKLDE      LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERKKALFSENAEKMKRLLDNTQQKLDESFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLS KA
Sbjct: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSIKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           AAAGDEDDCSSDRIL KRE+LESQIPALKGLV
Sbjct: 781 AAAGDEDDCSSDRILTKREYLESQIPALKGLV 812

BLAST of Carg12353 vs. ExPASy Swiss-Prot
Match: Q9LX73 (E3 UFM1-protein ligase 1 homolog OS=Arabidopsis thaliana OX=3702 GN=At3g46220 PE=1 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 3.4e-277
Identity = 508/813 (62.48%), Postives = 641/813 (78.84%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR+EI  EI KLGR+S+IDL+D IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  NGASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKECVDS FSQNS ISYE ++KLG+   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            FIH S++EMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ +LK+  AL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           I G+S++ S+ FIK +Y ++EKE +  ++  S+       S SS   ++ P+        
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSESTESIPA-------- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPS-TKSKKNQRKAKASSSVQV 480
                    N +K SKKKKGKS  +T++   E+  DD+E +  KSK+NQ+K + SSS Q 
Sbjct: 421 ---------NTDKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPM 540
            ++KAGGKKESVK +E++   P +EWV++KI   +P+ E+ G ++P  IL+ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHR 600
           L NS +ERRK +F+ENA++M+RL+D+ Q+KLDE      LY KALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNK 660
           HLLRTTAA I D L H LD+HNK+KNG EV E    + V L + ERT +AK+  GSLS K
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDP 720
           A+ + EALEGK +DTF+    DLAEESG+++KKLDKKLERTLLHSYRKDL SQVS E DP
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 IALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLI 780
           IALL KVVSLL+I+IH+KALQAPGRAI+ AIS LK+KLD+SA+K L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 780

Query: 781 SAAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           SA++G+E DCS+DRIL KRE LESQ+P L+ LV
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLV 795

BLAST of Carg12353 vs. ExPASy Swiss-Prot
Match: B8AXB6 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. indica OX=39946 GN=OsI_18219 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 1.3e-239
Identity = 449/815 (55.09%), Postives = 608/815 (74.60%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MD ELLELQRQ E AQ A+SS+RLSERNVVELVQKLQE  I+DFELLHT +GKEYIT +H
Sbjct: 1   MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           L+HEI  EI+K GR SL+DLSD +GVDLY++E+Q++++++DDP LMLI GEI+SQSYWD+
Sbjct: 61  LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           V EEINE+LQE SQIALAEIAA+L +GSEL+ ++L+ RLGT+VKGRLEGGQLYTPAYV+R
Sbjct: 121 VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           ++AMVRGA R ITVPTNL  +W+SLQ  LQ ++GASG++V+ SFFQS+FNGL+KE  +LG
Sbjct: 181 ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           S+RAGV WTP +F+ AQKE VD+FFSQNS I YE L+KL +P P Q+L++RYPDGI L  
Sbjct: 241 SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            F+HPS+V+MLD+ V D +E G W ++L VLPS     DA+K+L  CPS++ ++KS+ A+
Sbjct: 301 VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           +FG+S +FSN FIK ++ R+EKEM++  +  S+      +  S  +  +D     + +G 
Sbjct: 361 VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSD----GKDLG- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEP-STKSKKNQRKAKASSSVQV 480
            N++   G + +K  KKK+GK +G+ +  A E   D++E    K KK  RK K + S   
Sbjct: 421 DNDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS--S 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEE-HGIDDPAIILQPLVNHLRP 540
            + K GGKK S KTKE++     ++ + +K+ T+ P+LEE  G DD    L+ L +HLRP
Sbjct: 481 GDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLRP 540

Query: 541 MLNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILH 600
           ML ++W ++R  + SENAE+ +RLLDN Q++LDE      LY K+LD+FEDD + S ILH
Sbjct: 541 MLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAILH 600

Query: 601 RHLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSN 660
           +HLLRT  AP+VD +   L   NKLKNG++V +  + E V LST +RT++AK  PGSLS 
Sbjct: 601 KHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLSV 660

Query: 661 KAVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMD 720
           KA  +AE LEGK  D+F+DAL D AEESG++ KKLDK+LER++LHSYRKDLT+QVS+E D
Sbjct: 661 KAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRKDLTAQVSSEND 720

Query: 721 PIALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSL 780
           PI+ LPKVV+LL++Q ++KALQAPGRA+   I+ LKDK+    +K+L+DY + TV +L+L
Sbjct: 721 PISFLPKVVALLFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLAL 780

Query: 781 ISAAAGDEDDCSSDRILAKREFLESQI-PALKGLV 807
            +AA  D +DC++DR+L ++E LE ++ P LK LV
Sbjct: 781 QAAATEDGEDCATDRMLERKEDLEERLMPELKSLV 806

BLAST of Carg12353 vs. ExPASy Swiss-Prot
Match: B9FM64 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0117600 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 1.3e-239
Identity = 449/815 (55.09%), Postives = 608/815 (74.60%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MD ELLELQRQ E AQ A+SS+RLSERNVVELVQKLQE  I+DFELLHT +GKEYIT +H
Sbjct: 1   MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           L+HEI  EI+K GR SL+DLSD +GVDLY++E+Q++++++DDP LMLI GEI+SQSYWD+
Sbjct: 61  LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           V EEINE+LQE SQIALAEIAA+L +GSEL+ ++L+ RLGT+VKGRLEGGQLYTPAYV+R
Sbjct: 121 VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           ++AMVRGA R ITVPTNL  +W+SLQ  LQ ++GASG++V+ SFFQS+FNGL+KE  +LG
Sbjct: 181 ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           S+RAGV WTP +F+ AQKE VD+FFSQNS I YE L+KL +P P Q+L++RYPDGI L  
Sbjct: 241 SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            F+HPS+V+MLD+ V D +E G W ++L VLPS     DA+K+L  CPS++ ++KS+ A+
Sbjct: 301 VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           +FG+S +FSN FIK ++ R+EKEM++  +  S+      +  S  +  +D     + +G 
Sbjct: 361 VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSD----GKDLG- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEP-STKSKKNQRKAKASSSVQV 480
            N++   G + +K  KKK+GK +G+ +  A E   D++E    K KK  RK K + S   
Sbjct: 421 DNDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS--S 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEE-HGIDDPAIILQPLVNHLRP 540
            + K GGKK S KTKE++     ++ + +K+ T+ P+LEE  G DD    L+ L +HLRP
Sbjct: 481 GDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLRP 540

Query: 541 MLNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILH 600
           ML ++W ++R  + SENAE+ +RLLDN Q++LDE      LY K+LD+FEDD + S ILH
Sbjct: 541 MLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAILH 600

Query: 601 RHLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSN 660
           +HLLRT  AP+VD +   L   NKLKNG++V +  + E V LST +RT++AK  PGSLS 
Sbjct: 601 KHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLSV 660

Query: 661 KAVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMD 720
           KA  +AE LEGK  D+F+DAL D AEESG++ KKLDK+LER++LHSYRKDLT+QVS+E D
Sbjct: 661 KAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRKDLTAQVSSEND 720

Query: 721 PIALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSL 780
           PI+ LPKVV+LL++Q ++KALQAPGRA+   I+ LKDK+    +K+L+DY + TV +L+L
Sbjct: 721 PISFLPKVVALLFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLAL 780

Query: 781 ISAAAGDEDDCSSDRILAKREFLESQI-PALKGLV 807
            +AA  D +DC++DR+L ++E LE ++ P LK LV
Sbjct: 781 QAAATEDGEDCATDRMLERKEDLEERLMPELKSLV 806

BLAST of Carg12353 vs. ExPASy Swiss-Prot
Match: Q5ZMG1 (E3 UFM1-protein ligase 1 OS=Gallus gallus OX=9031 GN=UFL1 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.0e-71
Identity = 225/803 (28.02%), Postives = 387/803 (48.19%), Query Frame = 0

Query: 3   DELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEHLR 62
           +E+  L   F+ AQ A+++ RLSERN +E+V KL  +     E++HT+ GKEY+TP  + 
Sbjct: 6   EEIRRLAADFQRAQFAEAAHRLSERNCIEIVTKL--IAEKQLEVVHTLDGKEYVTPAQIS 65

Query: 63  HEILAEIEKL-GRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDSV 122
            EI  E+    GRI+++DL   I VDL +IE +A  I+  D  + L+ G++I++SY D +
Sbjct: 66  KEIWDELSVCGGRINIVDLQQIINVDLLHIENRANDIVKSDKAVQLVLGQLINESYLDQL 125

Query: 123 AEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLE---GGQLYTPAYV 182
           AEEIN++LQE+ Q+ ++E+     +  + L   L +RLG ++ GRL+    G ++T A+V
Sbjct: 126 AEEINDKLQETGQVTISELCKAYDLPGDFLTQALSKRLGRIIHGRLDQENRGVIFTEAFV 185

Query: 183 ARVSAMVRGATRAITVPT---NLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKE 242
           +R  A +RG   AIT PT   NL   +   + LL             S  + L N    +
Sbjct: 186 SRHRARIRGLFSAITRPTPVSNLITRYGFQEHLLY------------SVLEELVNTSRLK 245

Query: 243 NEILGSLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDG 302
             ++G  +    + P+I++  Q   VDSFF QN  + ++ L +LG+P+P  +++ RY   
Sbjct: 246 GTVVGGKQDKAVFVPDIYARTQSNWVDSFFKQNGYLEFDALYRLGIPDPAGYIKKRYKST 305

Query: 303 --IPLSTTFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVS 362
             + L    +   IV+ ++++V++++  GSW +   +LPSS   +D   +L     +R  
Sbjct: 306 KLLFLRAACVGQEIVDRVEASVDEVISSGSWIDVATLLPSSLSVEDIGILLQQV--MRSL 365

Query: 363 LKSNDALIFGDSFIFSNIFIK---DLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTD 422
            K++  L+F D+ + S  FI    DL++ M K+     +  S +   + + L        
Sbjct: 366 NKNSSGLVFSDTIVVSEKFISSCTDLFSDMMKQKAEKEMKNSPVHLITEEDLKQ------ 425

Query: 423 PSMFSESIGTGNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRK 482
            S   E+  T         N + K  +++ K+T  + S       + +E   K  K + +
Sbjct: 426 -SYVLENSYT---------NKKDKKDERRKKATEGSGSVRGGGGGNAREIKIKKTKKKGR 485

Query: 483 AKASSSVQVAETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQ 542
             A S  +   T  G      + K+ + ++ ++E + + +KT       H  D P  ++ 
Sbjct: 486 KDADSDEESQATGTG------RNKQLEFHFMSQEEMQDVLKT-------HLQDCPEELIT 545

Query: 543 PLVNHLRPMLNNSWRERRKALF----SENAEKMKRLLDNTQQKLDELYG------KALDL 602
            L  HL   L  S++E  +++F    S +    ++ + + Q++   LY       K    
Sbjct: 546 ELAEHLMRPLTKSYQEVVRSVFTSSTSSSGASGRQTMKDLQEEFSNLYNNIRLFEKGTKY 605

Query: 603 FEDDQSISVILHRHLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGE-R 662
           F D+   +  L +HLL+T    I +++F          N +    ++ +E  S  T E R
Sbjct: 606 FTDETQTN--LAKHLLKTVCTDITNLIF----------NFLASDSMMTTENYSTITSEVR 665

Query: 663 TTIAKSFPGSLSNKAVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSY 722
           T I    P         +  +L GK ++ F+  L+  A+   ++VKK DKK ER +L  +
Sbjct: 666 TKILGKLPEDTRGPLTKLHTSLNGKSLEDFLSYLDAAADICDIMVKKGDKKKERQVLFQH 725

Query: 723 RKDLTSQVSAEMDPIALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKIL 782
           R+ L  Q+    DP  +L     LL+    H  L APGR++   I+ L  K+ +    +L
Sbjct: 726 RQALIEQLKVTEDPALVLHLTAVLLFQFSTHCMLHAPGRSVPQIINFLSGKIPEDQHSLL 751

BLAST of Carg12353 vs. ExPASy Swiss-Prot
Match: Q8CCJ3 (E3 UFM1-protein ligase 1 OS=Mus musculus OX=10090 GN=Ufl1 PE=1 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 4.8e-69
Identity = 225/814 (27.64%), Postives = 389/814 (47.79%), Query Frame = 0

Query: 3   DELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEHLR 62
           +E+  L   F+ AQ A+S+ RLSERN +E+V KL   + L  E++HT+ GKEYITP  + 
Sbjct: 6   EEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQL--EVVHTLDGKEYITPAQIS 65

Query: 63  HEILAEIE-KLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDSV 122
            E+  E+  + GR++++DL   I VDL +IE +   II  +  + ++ G++I ++Y D +
Sbjct: 66  KEMRDELHVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDQL 125

Query: 123 AEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLE---GGQLYTPAYV 182
           +EE+N++LQES Q+ ++E+     +  + L   L QRLG ++ G L+    G ++T A+V
Sbjct: 126 SEEVNDKLQESGQVTVSELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFV 185

Query: 183 ARVSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEI 242
           AR  A +RG   AIT PT +  +              S          S+   LV    +
Sbjct: 186 ARHKARIRGLFSAITRPTPVNSL-------------VSKYGFQEQLLYSVLEDLVSTGRL 245

Query: 243 LGSLRAG----VHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPD 302
            G++  G      + P+I+S  Q   VDSFF QN  + ++ L +LG+P+ + +++ RY +
Sbjct: 246 RGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKN 305

Query: 303 G--IPLSTTFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRV 362
              + L  T +   +V+ ++++VE+ +  G+W +   +LPSS   +DA+ +L     +R 
Sbjct: 306 TQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQV--MRP 365

Query: 363 SLKSNDALIFGDSFIFSNIFIKD--------LYARMEKEMETINVPGSSIGTFSGDSLSS 422
             K   A++F D+ + S  FI D        ++ + EKEM+  N P   I          
Sbjct: 366 FGKLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMK--NNPVHLI---------- 425

Query: 423 CKFQTDPSMFSESIGTGNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKS 482
               T+  +   SI    N+ +  +  E++ K  +G  +G+ +     +A + +   TK 
Sbjct: 426 ----TEEDLKQISILESVNTSKKDKKDERRKKATEG--SGSVRGGGGGNAREYKIKKTKK 485

Query: 483 KKNQRKAKASSSVQVAETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDD 542
           K      K   S   +++  GGKK+       DI +  ++ + + ++  I D  E  I +
Sbjct: 486 KGR----KDEDSDDESQSSHGGKKKP------DITFMFQDEIEDCLRKHIQDAPEEFISE 545

Query: 543 PAIILQPLVNHLRPMLNNSWRERRKALF----SENAEKMKRLLDNTQQKLDELYG----- 602
                  L  +L   LN  + E  +++F    S +    KR + + Q+++  LY      
Sbjct: 546 -------LAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLF 605

Query: 603 -KALDLFEDDQSISVILHRHLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSL 662
            K +  F DD   +  L +HLL+T    I +++F      N L +   +A     EP ++
Sbjct: 606 EKGMKYFADDTQTA--LTKHLLKTVCTDITNLMF------NFLASDFLMAV---EEPAAI 665

Query: 663 STGERTTIAKSFPGSLSNKAVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERT 722
           ++  R  I              +  +L  K ++ F+  L+   E   ++VKK DKK ER 
Sbjct: 666 TSDIRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQ 725

Query: 723 LLHSYRKDLTSQVSAEMDPIALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDS 782
           +L  +R+ L  Q+    DP  +L     LL+    H  L APGR +   I+ L  K+ + 
Sbjct: 726 ILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSMLHAPGRCVPQIIAFLHSKIPED 756

Query: 783 AWKILSDYQTATVTLLSLISAAAGDEDDCSSDRI 789
              +L  YQ   V  L   +   G  +D SSD +
Sbjct: 786 QHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDEL 756

BLAST of Carg12353 vs. ExPASy TrEMBL
Match: A0A6J1GB94 (E3 UFM1-protein ligase 1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452413 PE=3 SV=1)

HSP 1 Score: 1490.3 bits (3857), Expect = 0.0e+00
Identity = 795/814 (97.67%), Postives = 800/814 (98.28%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSS KFQTDPSMFSESI T
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLD------ELYGKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFSENAEKMKRLLDNTQQKLD      +LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTA+PIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601 LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLVSS 809
           AAAGDEDDCSSDRIL KREFLESQIPALKGLV S
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLVFS 814

BLAST of Carg12353 vs. ExPASy TrEMBL
Match: A0A6J1GAG2 (E3 UFM1-protein ligase 1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452413 PE=3 SV=1)

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 794/812 (97.78%), Postives = 799/812 (98.40%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSS KFQTDPSMFSESI T
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSGRTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLD------ELYGKALDLFEDDQSISVILHRH 600
           NNSWRERRKALFSENAEKMKRLLDNTQQKLD      +LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTA+PIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601 LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           AAAGDEDDCSSDRIL KREFLESQIPALKGLV
Sbjct: 781 AAAGDEDDCSSDRILTKREFLESQIPALKGLV 812

BLAST of Carg12353 vs. ExPASy TrEMBL
Match: A0A6J1K905 (E3 UFM1-protein ligase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111492772 PE=3 SV=1)

HSP 1 Score: 1470.3 bits (3805), Expect = 0.0e+00
Identity = 787/812 (96.92%), Postives = 792/812 (97.54%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYY+EKQAEQIISDD RLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDSRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIW SLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG
Sbjct: 181 VSAMVRGATRAITVPTNLTVIWISLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFE QDASKVLLSCPSVRVSLKSNDAL
Sbjct: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEAQDASKVLLSCPSVRVSLKSNDAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLYARMEKEME INVPGSSIGTFSGDSLSS KFQTDPSMFSESI  
Sbjct: 361 IFGDSFIFSNIFIKDLYARMEKEMEAINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIEM 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNS RTGE MEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA
Sbjct: 421 GNNSCRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHRH 600
           NNSWRER+KALFSENAEKMKRLLDNTQQKLDE      LYGKALDLFEDDQSISVILHRH
Sbjct: 541 NNSWRERKKALFSENAEKMKRLLDNTQQKLDESFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAEL NSEPVSLSTGERTTIAKSFPGSLS KA
Sbjct: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSIKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGKLMDTFI+ALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           AAAGDEDDCSSDRIL KRE+LESQIPALKGLV
Sbjct: 781 AAAGDEDDCSSDRILTKREYLESQIPALKGLV 812

BLAST of Carg12353 vs. ExPASy TrEMBL
Match: A0A0A0LV94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G498310 PE=3 SV=1)

HSP 1 Score: 1370.1 bits (3545), Expect = 0.0e+00
Identity = 728/814 (89.43%), Postives = 766/814 (94.10%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 31  MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 90

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+D IGVDLYYIEKQAEQI+SDDP+L LIQGEIISQSYWDS
Sbjct: 91  LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 150

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 151 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 210

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRAITVPTNLTVIWS+LQQLLQGI+GASGIAVDASFFQSLFNG++KENE+LG
Sbjct: 211 VSAMVRGATRAITVPTNLTVIWSTLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 270

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTPNIFSIAQKE +DSFFSQNS+ISY+FLRKLG+PNPIQ+LQSRYPDGIPLST
Sbjct: 271 SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQYLQSRYPDGIPLST 330

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSI+EMLDST+EDILERGSW NSLLVLPSSFEPQDASK+LLSCPSV+ +LKSN AL
Sbjct: 331 TFIHPSIIEMLDSTIEDILERGSWANSLLVLPSSFEPQDASKILLSCPSVQGALKSNKAL 390

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSN FIKDLY RMEKEMETI VPGSS G FSGDS SS K   DPSM +ESI T
Sbjct: 391 IFGDSFIFSNTFIKDLYDRMEKEMETITVPGSSTGIFSGDSQSSSKLGNDPSMSTESIET 450

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GN+SG+TG+ M+KKSKKKKGKS GNTQSTAAE ALDDQE STKSKKNQRK + +S+VQVA
Sbjct: 451 GNDSGKTGDIMDKKSKKKKGKSIGNTQSTAAEGALDDQESSTKSKKNQRKTRGTSNVQVA 510

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKES KTKE++I YPTEEWVIEKIKTLIPDLEEHGIDDP II+QPL NHLRPML
Sbjct: 511 ETKAGGKKESAKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 570

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHRH 600
           NN WRERRKALF+ENAEKMKRLLDNTQQKLDE      LY KALDLFEDDQSISVILHRH
Sbjct: 571 NNLWRERRKALFTENAEKMKRLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 630

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRTTAAPIVDMLFHNLDL+NKLKNGIEVAEL NSE V+LSTGERTTIAKSFPGSLSNKA
Sbjct: 631 LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEAVALSTGERTTIAKSFPGSLSNKA 690

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGK ++TFI+AL DL EESGMI KKLDKKLERTLLHSYRK+LTSQVSAEMDPI
Sbjct: 691 VTVAEALEGKRVETFINALGDLVEESGMIPKKLDKKLERTLLHSYRKELTSQVSAEMDPI 750

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLY+QI+HKALQAPGRAIS AISRLKDKLDDSA KILSDYQTATVTLLSLIS
Sbjct: 751 ALLPKVVSLLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLIS 810

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLVSS 809
           AA GDEDDCSSDRIL KREFLESQIPALKGLV S
Sbjct: 811 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLS 844

BLAST of Carg12353 vs. ExPASy TrEMBL
Match: A0A6J1I5Q5 (E3 UFM1-protein ligase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469466 PE=3 SV=1)

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 726/814 (89.19%), Postives = 762/814 (93.61%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1   MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR EILAEIEKLGRISLIDL+DNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
             EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121 AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           VSAMVRGATRA TVPTNLTVIWSSLQQLL GI+GASGIAVD SFFQSLFNGLVKENE+LG
Sbjct: 181 VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAGVHWTP IFSIAQKEC+DSFFSQNSII+YEFLRKLG+PNPI FLQSRYPDGIPLST
Sbjct: 241 SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
           TFIHPSI+EMLDSTVEDILERGSWTNSLLVLPSSFEPQDASK+LLSCPSVRV+LK N A 
Sbjct: 301 TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           IFGDSFIFSNIFIKDLY RMEKEMET+NVPGSS   FSGDSLS  K   DPSM +ESI  
Sbjct: 361 IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480
           GNNSG+ GE MEKKSKKKKGKSTGNTQ+ AAE ALDDQE STKSKKNQRKAKA++SVQVA
Sbjct: 421 GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 480

Query: 481 ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540
           ETKAGGKKESVK KE++I YPTEEWVIEK+KTLIPDL+EHGIDDPA I+QPLVNHLRPML
Sbjct: 481 ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 540

Query: 541 NNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHRH 600
           NNSW+ERRKA F+ENAEKMKRLLD+TQQKLDE      LY KALDLFEDDQSISV+LHRH
Sbjct: 541 NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 600

Query: 601 LLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNKA 660
           LLRT+AAPIVDMLFHNLDL+NKLKNGIEVAE+ NSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 661 VTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720
           VTVAEALEGK ++TF+ AL DL EESGMI KKLDKKLERTLLHSYRKDLTSQVSAEMDPI
Sbjct: 661 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKDLTSQVSAEMDPI 720

Query: 721 ALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLIS 780
           ALLPKVVSLLY+QIHHKALQAPGRAIS AISRLKDKLD+ A+KILSDYQ+ATVTLLSLIS
Sbjct: 721 ALLPKVVSLLYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLIS 780

Query: 781 AAAGDEDDCSSDRILAKREFLESQIPALKGLVSS 809
           AA GDEDDCSSDRIL KREFLESQIPALKGLV S
Sbjct: 781 AAVGDEDDCSSDRILTKREFLESQIPALKGLVLS 814

BLAST of Carg12353 vs. TAIR 10
Match: AT3G46220.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611); Has 559 Blast hits to 533 proteins in 154 species: Archae - 3; Bacteria - 32; Metazoa - 305; Fungi - 42; Plants - 50; Viruses - 3; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 955.7 bits (2469), Expect = 2.4e-278
Identity = 508/813 (62.48%), Postives = 641/813 (78.84%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR+EI  EI KLGR+S+IDL+D IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  NGASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKECVDS FSQNS ISYE ++KLG+   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            FIH S++EMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ +LK+  AL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           I G+S++ S+ FIK +Y ++EKE +  ++  S+       S SS   ++ P+        
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSESTESIPA-------- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPS-TKSKKNQRKAKASSSVQV 480
                    N +K SKKKKGKS  +T++   E+  DD+E +  KSK+NQ+K + SSS Q 
Sbjct: 421 ---------NTDKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPM 540
            ++KAGGKKESVK +E++   P +EWV++KI   +P+ E+ G ++P  IL+ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHR 600
           L NS +ERRK +F+ENA++M+RL+D+ Q+KLDE      LY KALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNK 660
           HLLRTTAA I D L H LD+HNK+KNG EV E    + V L + ERT +AK+  GSLS K
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDP 720
           A+ + EALEGK +DTF+    DLAEESG+++KKLDKKLERTLLHSYRKDL SQVS E DP
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 IALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLI 780
           IALL KVVSLL+I+IH+KALQAPGRAI+ AIS LK+KLD+SA+K L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 780

Query: 781 SAAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           SA++G+E DCS+DRIL KRE LESQ+P L+ LV
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLV 795

BLAST of Carg12353 vs. TAIR 10
Match: AT3G46220.2 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 948.3 bits (2450), Expect = 3.9e-276
Identity = 508/821 (61.88%), Postives = 641/821 (78.08%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR+EI  EI KLGR+S+IDL+D IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  NGASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+ F+ AQKECVDS FSQNS ISYE ++KLG+   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            FIH S++EMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ +LK+  AL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           I G+S++ S+ FIK +Y ++EKE +  ++  S+       S SS   ++ P+        
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSESTESIPA-------- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPS-TKSKKNQRKAKASSSVQV 480
                    N +K SKKKKGKS  +T++   E+  DD+E +  KSK+NQ+K + SSS Q 
Sbjct: 421 ---------NTDKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHG--------IDDPAIILQP 540
            ++KAGGKKESVK +E++   P +EWV++KI   +P+ E+ G         ++P  IL+ 
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTSENFFLCTENPDSILKH 540

Query: 541 LVNHLRPMLNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQ 600
           L +H++PML NS +ERRK +F+ENA++M+RL+D+ Q+KLDE      LY KALDLFEDDQ
Sbjct: 541 LADHMKPMLINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQ 600

Query: 601 SISVILHRHLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKS 660
           S +V+LHRHLLRTTAA I D L H LD+HNK+KNG EV E    + V L + ERT +AK+
Sbjct: 601 STAVVLHRHLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKN 660

Query: 661 FPGSLSNKAVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTS 720
             GSLS KA+ + EALEGK +DTF+    DLAEESG+++KKLDKKLERTLLHSYRKDL S
Sbjct: 661 LNGSLSKKALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLIS 720

Query: 721 QVSAEMDPIALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTA 780
           QVS E DPIALL KVVSLL+I+IH+KALQAPGRAI+ AIS LK+KLD+SA+K L+DYQTA
Sbjct: 721 QVSTESDPIALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTA 780

Query: 781 TVTLLSLISAAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           TVTLL+L+SA++G+E DCS+DRIL KRE LESQ+P L+ LV
Sbjct: 781 TVTLLALMSASSGEEHDCSADRILTKRELLESQMPLLRTLV 803

BLAST of Carg12353 vs. TAIR 10
Match: AT3G46220.3 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 921.8 bits (2381), Expect = 3.9e-268
Identity = 496/813 (61.01%), Postives = 628/813 (77.24%), Query Frame = 0

Query: 1   MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60
           MDDELLELQRQFEFAQQ KSS+RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1   MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 61  LRHEILAEIEKLGRISLIDLSDNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120
           LR+EI  EI KLGR+S+IDL+D IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61  LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 121 VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180
           +AEEINERLQE SQIA+AE+A +LQVGSEL+ S+L+ RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121 IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 181 VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240
           V+AMVRGA+R I VP+NL+ +W+ LQQL+Q  NGASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181 VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 241 SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300
           SLRAG HWTP+                NS ISYE ++KLG+   +QFLQSRYPDG PL+ 
Sbjct: 241 SLRAGTHWTPS----------------NSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 301 TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360
            FIH S++EMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ +LK+  AL
Sbjct: 301 VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 361 IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSCKFQTDPSMFSESIGT 420
           I G+S++ S+ FIK +Y ++EKE +  ++  S+       S SS   ++ P+        
Sbjct: 361 ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKSSESTESIPA-------- 420

Query: 421 GNNSGRTGENMEKKSKKKKGKSTGNTQSTAAESALDDQEPS-TKSKKNQRKAKASSSVQV 480
                    N +K SKKKKGKS  +T++   E+  DD+E +  KSK+NQ+K + SSS Q 
Sbjct: 421 ---------NTDKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 481 AETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPM 540
            ++KAGGKKESVK +E++   P +EWV++KI   +P+ E+ G ++P  IL+ L +H++PM
Sbjct: 481 LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 541 LNNSWRERRKALFSENAEKMKRLLDNTQQKLDE------LYGKALDLFEDDQSISVILHR 600
           L NS +ERRK +F+ENA++M+RL+D+ Q+KLDE      LY KALDLFEDDQS +V+LHR
Sbjct: 541 LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 601 HLLRTTAAPIVDMLFHNLDLHNKLKNGIEVAELLNSEPVSLSTGERTTIAKSFPGSLSNK 660
           HLLRTTAA I D L H LD+HNK+KNG EV E    + V L + ERT +AK+  GSLS K
Sbjct: 601 HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 661 AVTVAEALEGKLMDTFIDALEDLAEESGMIVKKLDKKLERTLLHSYRKDLTSQVSAEMDP 720
           A+ + EALEGK +DTF+    DLAEESG+++KKLDKKLERTLLHSYRKDL SQVS E DP
Sbjct: 661 ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRKDLISQVSTESDP 720

Query: 721 IALLPKVVSLLYIQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLI 780
           IALL KVVSLL+I+IH+KALQAPGRAI+ AIS LK+KLD+SA+K L+DYQTATVTLL+L+
Sbjct: 721 IALLAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALM 779

Query: 781 SAAAGDEDDCSSDRILAKREFLESQIPALKGLV 807
           SA++G+E DCS+DRIL KRE LESQ+P L+ LV
Sbjct: 781 SASSGEEHDCSADRILTKRELLESQMPLLRTLV 779