Carg12349 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12349
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	chitinase 2-like
Location	Carg_Chr01: 3076702 .. 3078270 (+)
RNA-Seq Expression	Carg12349
Synteny	Carg12349

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGTAATCAAACGTTCAACAACCCAATATTATACCTAAAAAAAATATATCCTTTTTTGAGGATAAACTCTCGTAGCTTTGTTTTTTGTTTCTTCGAAAGATCTCGTACCAATGGAGATACTCTTATTTGTTGGATGATGAAAGTTTCACGTCCACTAATTTAGAGAATGATTCTGAGTTTCTAATCAATGAATACTCTTTCCATTGGTACGAGGTCTTTTGGGAAGCCCAAAGCAAAGCCACTAGAGCTTATGCTTAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATTATTTACTTATAAACTCACGATCTTCCTCTTAATTAGCTAATGTGGGACTACTCTTTTAATAATCCTCCCCTCGAGCAAAATACACCATAGAGCCTCCCCCTGAGGCCTATGAAGCCCTCGAACAAAGTACATCATTTGTTCGACACTCGAGTCACTTTTGACTCTAATACTATGTTAGGAATCACGACTCTCCACAATAATATGATATTGTCTACTTTGAGTATAAGCTCTCGTAGGTTTGTTTTTCGTTTCTCCAAAAAGTCTCGTGTCGTTAGAGATAGTATTCCTTACTTATAAACTCATAATTGTTCTCTTAATTACCGAACGAAATTACTCGTGCTTTAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

mRNA sequence

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

Coding sequence (CDS)

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

Protein sequence

MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPNYHY

Homology

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BLAST of Carg12349 vs. NCBI nr
Match: KAG6607221.1 (hypothetical protein SDJN03_00563, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036906.1 hypothetical protein SDJN02_00526, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621.7 bits (1602), Expect = 3.4e-174
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 0


Query: 1   MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60
           MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS
Sbjct: 1   MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60

Query: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYF 120
           FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYF
Sbjct: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYF 120

Query: 121 KPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180
           KPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS
Sbjct: 121 KPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180

Query: 181 FASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDG 240
           FASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDG
Sbjct: 181 FASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDG 240

Query: 241 RILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQ 300
           RILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQ
Sbjct: 241 RILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQ 300

Query: 301 SPNYHY 307
           SPNYHY
Sbjct: 301 SPNYHY 306

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BLAST of Carg12349 vs. NCBI nr
Match: XP_022949055.1 (chitinase 2-like [Cucurbita moschata])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 299/304 (98.36%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 3   MDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA 62
           MDGIIVVLLLILVAPPSNTAVVVPA VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA
Sbjct: 1   MDGIIVVLLLILVAPPSNTAVVVPAAVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA 60

Query: 63  IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP 122
           IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP
Sbjct: 61  IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP 120

Query: 123 SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA 182
           SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA
Sbjct: 121 SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA 180

Query: 183 SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRI 242
           SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSS YE GRI
Sbjct: 181 SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSNYEGGRI 240

Query: 243 LASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSP 302
           LASLASDDSGGLL KHGFFKACRRLRTDKLLHGIFIWSADDSQA GFRYEKKAQALLQSP
Sbjct: 241 LASLASDDSGGLLSKHGFFKACRRLRTDKLLHGIFIWSADDSQAHGFRYEKKAQALLQSP 300

Query: 303 NYHY 307
           NYHY
Sbjct: 301 NYHY 304

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BLAST of Carg12349 vs. NCBI nr
Match: XP_023524573.1 (chitinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.7 bits (1545), Expect = 1.4e-167
Identity = 295/305 (96.72%), Postives = 297/305 (97.38%), Query Frame = 0


Query: 1   MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60
           MEMDGIIVVLLLILVAPPS  AVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS
Sbjct: 1   MEMDGIIVVLLLILVAPPSTAAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60

Query: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYF 120
           FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKK+HSNVRVGVSLGGDSVGDSGFVYF
Sbjct: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKNHSNVRVGVSLGGDSVGDSGFVYF 120

Query: 121 KPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180
           KPSSIASWVSNAERSLTKLINTF LDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS
Sbjct: 121 KPSSIASWVSNAERSLTKLINTFKLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180

Query: 181 FASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDG 240
           FASIAPFDD EVQ HYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSS YE G
Sbjct: 181 FASIAPFDDTEVQAHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSNYEGG 240

Query: 241 RILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQ 300
           RILASLASDD+GGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQA GFRYEKKAQALLQ
Sbjct: 241 RILASLASDDTGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQAHGFRYEKKAQALLQ 300

Query: 301 SPNYH 306
           SPNYH
Sbjct: 301 SPNYH 305

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BLAST of Carg12349 vs. NCBI nr
Match: XP_038895814.1 (chitinase 2-like [Benincasa hispida])

HSP 1 Score: 487.6 bits (1254), Expect = 7.6e-134
Identity = 239/290 (82.41%), Postives = 261/290 (90.00%), Query Frame = 0


Query: 14  LVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFT 73
           +VAPPS+ A      VFREYIGAEF DVKFSDVPINPNVEEFHFLLSFAIDFDSSRR FT
Sbjct: 1   MVAPPSH-AAAGRGNVFREYIGAEFKDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRSFT 60

Query: 74  NGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAE 133
           NGVFNVFWDS NLSPSAVA+IK+DHSNVRVGVSLGGDSV   G V+FKPSSIASWVSNAE
Sbjct: 61  NGVFNVFWDSDNLSPSAVASIKRDHSNVRVGVSLGGDSVDKGGSVFFKPSSIASWVSNAE 120

Query: 134 RSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQ 193
            SLT+LINT+NLDGIDIDYEHF+SDP+TFA+CIGQLI RLKKKG+ISFASIAPFD AEVQ
Sbjct: 121 TSLTELINTYNLDGIDIDYEHFKSDPSTFAKCIGQLIGRLKKKGIISFASIAPFDKAEVQ 180

Query: 194 THYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGG 253
           +HYLALW+ YGHLIDYVNFQFYAYDK TTV QFI+HFKAQ S YE GR+LASLASDDSGG
Sbjct: 181 SHYLALWRSYGHLIDYVNFQFYAYDKGTTVGQFIQHFKAQLSNYEGGRVLASLASDDSGG 240

Query: 254 LLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           L+P +GFF+ACR+L+T+KLL GIFIWSADDSQ+ GF YEKKAQALL  PN
Sbjct: 241 LVPDNGFFRACRKLKTEKLLDGIFIWSADDSQSNGFGYEKKAQALLAMPN 289

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BLAST of Carg12349 vs. NCBI nr
Match: KGN65683.1 (hypothetical protein Csa_019605 [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 237/297 (79.80%), Postives = 265/297 (89.23%), Query Frame = 0


Query: 7   IVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFD 66
           ++ LL +LVAPPS  A   PA VFREYIGAEFN+VKFSDVPI+ NVEEFHFLLSFAIDFD
Sbjct: 15  LLFLLQLLVAPPSLMA-AGPANVFREYIGAEFNNVKFSDVPIHSNVEEFHFLLSFAIDFD 74

Query: 67  SSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIA 126
           +S R FTNG F+VFWDS NL+PSAVA+IKKDHSNVRVGVSLGGD+V + G V+FKPSSI 
Sbjct: 75  ASSRSFTNGAFDVFWDSDNLNPSAVASIKKDHSNVRVGVSLGGDTVDNGGSVFFKPSSIH 134

Query: 127 SWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAP 186
           SWVSNAE SLTK+I T+NLDGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAP
Sbjct: 135 SWVSNAEHSLTKIIKTYNLDGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAP 194

Query: 187 FDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASL 246
           FDD EVQ+HYLALWKRYGHLIDYVNFQFYAYDK TT+ QFI+HFK Q S YE GRILASL
Sbjct: 195 FDDDEVQSHYLALWKRYGHLIDYVNFQFYAYDKGTTIGQFIQHFKDQMSNYEGGRILASL 254

Query: 247 ASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
            SDDSGGL+PK+GFFKACRRL+ +KLL+GIFIWSADDSQA+GF YEK AQ+LL  PN
Sbjct: 255 VSDDSGGLVPKNGFFKACRRLKKEKLLNGIFIWSADDSQAKGFDYEKTAQSLLAKPN 310

[Close]

BLAST of Carg12349 vs. ExPASy Swiss-Prot
Match: Q9SLP4 (Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.2e-101
Identity = 184/297 (61.95%), Postives = 227/297 (76.43%), Query Frame = 0


Query: 10  LLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDS-S 69
           LLL+  A  S    +  A VFREYIG++FNDVKFSDVPINP+V +FHF+L+FAID+ S S
Sbjct: 9   LLLLFFALLSPLLPLTSALVFREYIGSQFNDVKFSDVPINPDV-DFHFILAFAIDYTSGS 68

Query: 70  RRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASW 129
               TNG F  FWD+ NLSPS VAA+K+ HSNV+V +SLGGDSVG    V+F PSS++SW
Sbjct: 69  SPTPTNGNFKPFWDTNNLSPSQVAAVKRTHSNVKVSLSLGGDSVGGKN-VFFSPSSVSSW 128

Query: 130 VSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFD 189
           V NA  SLT++I  ++LDGIDIDYEHF+ DPNTFAECIGQL+ RLKK  V+SF SIAPFD
Sbjct: 129 VENAVSSLTRIIKQYHLDGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSFVSIAPFD 188

Query: 190 DAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLAS 249
           DA+VQ+HY ALW++YGH IDYVNFQFYAY   T+V QF+K+F+ QSS Y  G++L S ++
Sbjct: 189 DAQVQSHYQALWEKYGHQIDYVNFQFYAYSARTSVEQFLKYFEEQSSNYHGGKVLVSFST 248

Query: 250 DDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARG--FRYEKKAQALLQSPN 304
           D SGGL P +GFF+AC  L+    LHGIF+WSADDS      FRYE +AQ++L S N
Sbjct: 249 DSSGGLKPDNGFFRACSILKKQGKLHGIFVWSADDSLMSNNVFRYEMQAQSMLASVN 303

[Close]

BLAST of Carg12349 vs. ExPASy Swiss-Prot
Match: Q7M443 (Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.2e-99
Identity = 177/276 (64.13%), Postives = 218/276 (78.99%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDS-SRRCFTNGVFNVFWDSYNLS 88
           +FREYIGA+FNDVKFSDVPINPNV +FHF+L+FAID+ S S    TNG FN FWD+ NLS
Sbjct: 2   LFREYIGAQFNDVKFSDVPINPNV-DFHFILAFAIDYTSGSSPTPTNGNFNPFWDTNNLS 61

Query: 89  PSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDG 148
           PS VAAIK+ ++NV+V VSLGG+SVG    V+F PSS++SWV NA  SLTK+I  ++LDG
Sbjct: 62  PSQVAAIKRTYNNVKVSVSLGGNSVGGER-VFFNPSSVSSWVDNAVSSLTKIIKQYHLDG 121

Query: 149 IDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLI 208
           IDIDYEHF+ DPNTFAECIGQL+ RLKK GV+SF SIAPFDDA+VQ+HY ALW++YGH I
Sbjct: 122 IDIDYEHFKGDPNTFAECIGQLVTRLKKNGVVSFVSIAPFDDAQVQSHYQALWEKYGHQI 181

Query: 209 DYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRL 268
           DYVNFQFY Y    +V QF+K+F+ Q S Y  G++L S ++D+SGGL P++GFF AC  L
Sbjct: 182 DYVNFQFYVYSSRMSVEQFLKYFEMQRSNYPGGKVLVSFSTDNSGGLKPRNGFFDACSIL 241

Query: 269 RTDKLLHGIFIWSADDSQARG--FRYEKKAQALLQS 302
           +    LHGIF+WSADDS      F+YE +AQ+LL S
Sbjct: 242 KKQGKLHGIFVWSADDSLMSNDVFKYEMQAQSLLAS 275

[Close]

BLAST of Carg12349 vs. ExPASy Swiss-Prot
Match: P39657 (RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.9e-21
Identity = 65/188 (34.57%), Postives = 93/188 (49.47%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 88
           VFRE+     +D  F +  I  N+ EF   LS A D+D +    TNG F  +WD+  ++P
Sbjct: 7   VFREFT----SDDSFLNQVIPENITEFQVTLSLARDYDGNNS--TNGKFIPYWDTEKVTP 66

Query: 89  SAVAAIKKDHS----NVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFN 148
             +   KK +      V+V VS+G  +     F     S+  +WVS A  SL  +I T+N
Sbjct: 67  EVIKKFKKKYEPTALRVKVLVSIGNKN-KQFPFTIGSDSNSEAWVSEATASLKSIIKTYN 126

Query: 149 LDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYG 208
           LDGID+ YE   ++   F   +G L+R LK+  +I+ AS A   DA     Y  L+  Y 
Sbjct: 127 LDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYA 186

Query: 209 HLIDYVNF 213
              D V F
Sbjct: 187 TFFDTVVF 187

[Close]

BLAST of Carg12349 vs. ExPASy TrEMBL
Match: A0A6J1GBP9 (chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452519 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.1e-170
Identity = 299/304 (98.36%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 3   MDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA 62
           MDGIIVVLLLILVAPPSNTAVVVPA VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA
Sbjct: 1   MDGIIVVLLLILVAPPSNTAVVVPAAVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFA 60

Query: 63  IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP 122
           IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP
Sbjct: 61  IDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKP 120

Query: 123 SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA 182
           SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA
Sbjct: 121 SSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFA 180

Query: 183 SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRI 242
           SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSS YE GRI
Sbjct: 181 SIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSNYEGGRI 240

Query: 243 LASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSP 302
           LASLASDDSGGLL KHGFFKACRRLRTDKLLHGIFIWSADDSQA GFRYEKKAQALLQSP
Sbjct: 241 LASLASDDSGGLLSKHGFFKACRRLRTDKLLHGIFIWSADDSQAHGFRYEKKAQALLQSP 300

Query: 303 NYHY 307
           NYHY
Sbjct: 301 NYHY 304

[Close]

BLAST of Carg12349 vs. ExPASy TrEMBL
Match: A0A0A0LV90 (Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496300 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 237/297 (79.80%), Postives = 265/297 (89.23%), Query Frame = 0


Query: 7   IVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFD 66
           ++ LL +LVAPPS  A   PA VFREYIGAEFN+VKFSDVPI+ NVEEFHFLLSFAIDFD
Sbjct: 15  LLFLLQLLVAPPSLMA-AGPANVFREYIGAEFNNVKFSDVPIHSNVEEFHFLLSFAIDFD 74

Query: 67  SSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIA 126
           +S R FTNG F+VFWDS NL+PSAVA+IKKDHSNVRVGVSLGGD+V + G V+FKPSSI 
Sbjct: 75  ASSRSFTNGAFDVFWDSDNLNPSAVASIKKDHSNVRVGVSLGGDTVDNGGSVFFKPSSIH 134

Query: 127 SWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAP 186
           SWVSNAE SLTK+I T+NLDGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAP
Sbjct: 135 SWVSNAEHSLTKIIKTYNLDGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAP 194

Query: 187 FDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASL 246
           FDD EVQ+HYLALWKRYGHLIDYVNFQFYAYDK TT+ QFI+HFK Q S YE GRILASL
Sbjct: 195 FDDDEVQSHYLALWKRYGHLIDYVNFQFYAYDKGTTIGQFIQHFKDQMSNYEGGRILASL 254

Query: 247 ASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
            SDDSGGL+PK+GFFKACRRL+ +KLL+GIFIWSADDSQA+GF YEK AQ+LL  PN
Sbjct: 255 VSDDSGGLVPKNGFFKACRRLKKEKLLNGIFIWSADDSQAKGFDYEKTAQSLLAKPN 310

[Close]

BLAST of Carg12349 vs. ExPASy TrEMBL
Match: A0A6J1DUG9 (chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111023583 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.7e-131
Identity = 240/309 (77.67%), Postives = 267/309 (86.41%), Query Frame = 0


Query: 1   MEMDG------IIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEE 60
           MEMDG      I+V L L+L+ PPS+ A    A +FREYIGAEF DVKFSDVPINP+V E
Sbjct: 1   MEMDGSGIRAFILVALQLLLLFPPSHAA----AKLFREYIGAEFKDVKFSDVPINPDV-E 60

Query: 61  FHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGD 120
           FHFLLSFAIDFD+SRR FTNG+FNVFWDS+NL+PS V AIKKDHSNV+VG+S+GGD+V  
Sbjct: 61  FHFLLSFAIDFDASRRSFTNGIFNVFWDSHNLNPSDVVAIKKDHSNVKVGLSIGGDTV-P 120

Query: 121 SGFVYFKPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLK 180
           +GF YFKPSSI SWVSNAERSLTKLIN FNLD IDIDYEHF+SDPNTFAECIG+LI+RLK
Sbjct: 121 NGFAYFKPSSIDSWVSNAERSLTKLINAFNLDSIDIDYEHFKSDPNTFAECIGRLIKRLK 180

Query: 181 KKGVISFASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQS 240
           KKG ISFASIAPFD AEVQ+HYLALW+ YGHLIDYVNFQFYAYDKTTTV QFI+HFKAQ+
Sbjct: 181 KKGAISFASIAPFDKAEVQSHYLALWRSYGHLIDYVNFQFYAYDKTTTVPQFIQHFKAQT 240

Query: 241 SKYEDGRILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKK 300
           S YE GR+LASL SDDSGGLL ++GFF ACRRLR +KLLHGIF+WSADDS A GF YEKK
Sbjct: 241 SNYEGGRVLASLVSDDSGGLLAENGFFTACRRLRAEKLLHGIFVWSADDSIANGFGYEKK 300

Query: 301 AQALLQSPN 304
           AQ LL  PN
Sbjct: 301 AQELLAMPN 303

[Close]

BLAST of Carg12349 vs. ExPASy TrEMBL
Match: A0A5A7SWP3 (Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00520 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.2e-130
Identity = 233/298 (78.19%), Postives = 266/298 (89.26%), Query Frame = 0


Query: 7   IVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFD 66
           ++ LL + VAPPS  A   PA VFREYIGAEFN+VKFSDVPI  N+EEFHFLLSFAIDFD
Sbjct: 15  LLFLLQLFVAPPSLMA-AGPANVFREYIGAEFNNVKFSDVPIRSNIEEFHFLLSFAIDFD 74

Query: 67  SSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIA 126
           +S R FTNG F+VFWDS NL+PSAVA+IKKDHSNVRVGVSLGGD+V + GFV+FKPSSI 
Sbjct: 75  ASSRSFTNGAFDVFWDSDNLNPSAVASIKKDHSNVRVGVSLGGDTVDNGGFVFFKPSSIH 134

Query: 127 SWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAP 186
           SWVSNAE SLTK+INT+NLDGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAP
Sbjct: 135 SWVSNAEHSLTKIINTYNLDGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAP 194

Query: 187 FDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASL 246
           FDD EVQ+HYLALW+ YGHLIDYVNFQFYAYDK TTV QFI+HFK Q S YE GR+LASL
Sbjct: 195 FDDDEVQSHYLALWRSYGHLIDYVNFQFYAYDKGTTVGQFIRHFKDQVSNYEGGRVLASL 254

Query: 247 ASDDS-GGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           ASD+S GGL+P++GFFKACRRL+ +KLL GIFIWSADDS+A+GF YEK+AQ+LL +PN
Sbjct: 255 ASDNSAGGLVPENGFFKACRRLKREKLLDGIFIWSADDSKAKGFGYEKEAQSLLATPN 311

[Close]

BLAST of Carg12349 vs. ExPASy TrEMBL
Match: A0A1S3C5A3 (chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103497192 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.2e-130
Identity = 233/298 (78.19%), Postives = 266/298 (89.26%), Query Frame = 0


Query: 7   IVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFD 66
           ++ LL + VAPPS  A   PA VFREYIGAEFN+VKFSDVPI  N+EEFHFLLSFAIDFD
Sbjct: 15  LLFLLQLFVAPPSLMA-AGPANVFREYIGAEFNNVKFSDVPIRSNIEEFHFLLSFAIDFD 74

Query: 67  SSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIA 126
           +S R FTNG F+VFWDS NL+PSAVA+IKKDHSNVRVGVSLGGD+V + GFV+FKPSSI 
Sbjct: 75  ASSRSFTNGAFDVFWDSDNLNPSAVASIKKDHSNVRVGVSLGGDTVDNGGFVFFKPSSIH 134

Query: 127 SWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAP 186
           SWVSNAE SLTK+INT+NLDGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAP
Sbjct: 135 SWVSNAEHSLTKIINTYNLDGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAP 194

Query: 187 FDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASL 246
           FDD EVQ+HYLALW+ YGHLIDYVNFQFYAYDK TTV QFI+HFK Q S YE GR+LASL
Sbjct: 195 FDDDEVQSHYLALWRSYGHLIDYVNFQFYAYDKGTTVGQFIRHFKDQVSNYEGGRVLASL 254

Query: 247 ASDDS-GGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           ASD+S GGL+P++GFFKACRRL+ +KLL GIFIWSADDS+A+GF YEK+AQ+LL +PN
Sbjct: 255 ASDNSAGGLVPENGFFKACRRLKREKLLDGIFIWSADDSKAKGFGYEKEAQSLLATPN 311

The following BLAST results are available for this feature:

BLAST of Carg12349 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6607221.1	3.4e-174	100.00	hypothetical protein SDJN03_00563, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022949055.1	1.5e-169	98.36	chitinase 2-like [Cucurbita moschata]	[more]
XP_023524573.1	1.4e-167	96.72	chitinase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_038895814.1	7.6e-134	82.41	chitinase 2-like [Benincasa hispida]	[more]
KGN65683.1	3.2e-132	79.80	hypothetical protein Csa_019605 [Cucumis sativus]	[more]

BLAST of Carg12349 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLP4	5.2e-101	61.95	Chitinase 1 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1	[more]
Q7M443	2.2e-99	64.13	Chitinase 2 OS=Tulipa saxatilis subsp. bakeri OX=110455 PE=1 SV=1	[more]
P39657	6.9e-21	34.57	RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of Carg12349 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBP9	7.1e-170	98.36	chitinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452519 PE=3 SV=1	[more]
A0A0A0LV90	1.5e-132	79.80	Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496...	[more]
A0A6J1DUG9	1.7e-131	77.67	chitinase 2-like OS=Momordica charantia OX=3673 GN=LOC111023583 PE=4 SV=1	[more]
A0A5A7SWP3	4.2e-130	78.19	Chitinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G0052...	[more]
A0A1S3C5A3	4.2e-130	78.19	chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103497192 PE=3 SV=1	[more]

BLAST of Carg12349 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000677	Chitinase-like	PRINTS	PR00551	2SGLOBULIN	coord: 27..40 score: 46.43 coord: 74..89 score: 27.6 coord: 91..109 score: 40.35 coord: 262..281 score: 37.08
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 26..301 e-value: 7.4E-83 score: 280.2
None	No IPR available	PANTHER	PTHR46476	CHITINASE 2-LIKE	coord: 14..301
None	No IPR available	PANTHER	PTHR46476:SF4	2, PUTATIVE, EXPRESSED-RELATED	coord: 14..301
None	No IPR available	CDD	cd06544	GH18_narbonin	coord: 29..284 e-value: 3.69524E-122 score: 348.592
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 89..224 e-value: 3.1E-14 score: 53.4
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 28..306 score: 23.559898
IPR001579	Glycosyl hydrolases family 18 (GH18) active site	PROSITE	PS01095	GH18_1	coord: 145..153
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 28..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12349-RA	Carg12349-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds