Carg12329 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12329
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionL-ascorbate peroxidase
LocationCarg_Chr01: 2978070 .. 2979970 (+)
RNA-Seq ExpressionCarg12329
SyntenyCarg12329
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTTGATATGAAAATGTGGATGTTGATTTGTTTGATTTCAGTATCATCGTCAATTTCAGCATGACAAAGTGCTACCCTGTTGTAAGTGAGGAATATCAAAAGGCCGTTGAAAAGGCCAAGAGGAAGCTTAGAGGACTCATTGCTGAGAAGAATTGTGCTCCTCTAATGCTCCGTCTTGCGTATGTTCTTGTTGTTCTTGATTTCTGAGTTCTTAGTTTTTCCCTATTTTTGTGAGTGATTGTGGTTTTATTTTTTCAGATGGCACTCTGCTGGAACCTTTGACCATAAATCCAAGACTGGTGGTCCGTTTGGAACTATGAGGTTCCCTAGCGAGCTCGCTCATGCAGCCAACAGTGGCCTTGACATCGCTATTAGGCTATTGGAGCCTATCAAGGAACAGTTCCCGATCCTCACATATGCTGACTTTTACCAGGTAATTTCTCTGATCTGATCTGTTTTTTGTGGACTTTTGATCTTGATTGTTGTTTAATTTTGTGTTTCTGGGTTTGATTTCTTCCAGTTGGCTGGTGTTGTTGCTGTCGAGATCACTGGAGGGCCTGAGGTTCCTTTCCACCCTGGTAGAGAGGTCAGTGATATCCAATTATCTGTTCTATTTGATTGGTGTAGCGTTTGTTCTTAGTTGAGAATGGATTTGGATATCTGGATTGTTTCAACTTTTAATGTTTATTCATTATGATCTGAAACCATAGTAACCAGATTGAATCAGAGCTCAAGTAATGCAAACTAGCAATGATAATTTAACCTGTTCTTGACTTAATGAACTGTTCTGATATTGAAAATTGGTGTTCACTGTATGCAGGACAAGCCCGCGCCACCACCGGAAGGTCGCTTGCCCGATGCTACCAAGGGTTAGTATCCATGACAATATGGTTGTTATGTGAATGGTGAAGACGAATTTTTAGATTAGAAAATTAACTGATGGGTTGTGTTCTGTTCAGGTTCTGATCATTTGAGGGATGTTTTCTACACCATGGGCCTCTCTGACCAGGACATTGTCGCCCTCTCTGGCGGCCACACATTGGTTCGTTCCGTTGAAATTTAGTTCTTGAGCTTTGATGATTAGTGATATAGAAATGAATCCATGAACTGGAATTTAGTATAGTCAAGCAGTTCGAGTTTTAGAAGCTAATGATGATTTAACTCGACAGGGTCGGGCACACAAGGAACGATCCGGGTTTGAGGGACCATGGACTTCCAACCCTCTCATCTTCGACAACTCGTATTTCACGTGAGTTAAAACCAGTTTATTTATAAATGGAGCTTGTGAAGAAATGTTGAAAGCTCTAATTCTTTTATGTGAACATGCTTGTTTACGACAGCGAGCTCTTGACCGGGGAGAAGGAAGGTCTTCTGCAGTTGGTATCGGACAAGACACTTCTATGCGACCCTGTCTTCCGCCCTCTCGTTGAAAAATATGCTGCAGATGAGGATGCTTTCTTTGCTGATTATGCTGAGGCTCACCAGAAGCTTTCTGAGCTCGGGTAAGACTTTTCGAACTCAAGTTTAACGTGTTCTCTTGTCGTCCATCGTGTTGTCCATTGATAGCTCGAGAGATAATAAAGAGGACAAGTTCATAACTTAGTGAAATTTCATTTGCAGATTTGCTGATGCTTGAGCAATGGAAATGGAAAAATGAGGCTGCAGGCTGTTGTTGTTTATGTTAGCTTTGATTTGTAATGAAATGGGTAGGTTGGGTTGGGTTGGGTGGCCCTGTCTTTTTTTGTTGATTTGAATTTTGGATTTTGCCTCATCTCCCAGCCTCTTTGGACTTGAATAAAGTTGCTTCAAAGTTTATCATTTTGGTGTACTTTTTAGGCATAAACCAATCAATATCTTGAATGTTTTGAGCCCCAAATGCTTGCAAGTGGGTCAATTTGGC

mRNA sequence

GATTTGATATGAAAATGTGGATGTTGATTTGTTTGATTTCAGTATCATCGTCAATTTCAGCATGACAAAGTGCTACCCTGTTGTAAGTGAGGAATATCAAAAGGCCGTTGAAAAGGCCAAGAGGAAGCTTAGAGGACTCATTGCTGAGAAGAATTGTGCTCCTCTAATGCTCCGTCTTGCATGGCACTCTGCTGGAACCTTTGACCATAAATCCAAGACTGGTGGTCCGTTTGGAACTATGAGGTTCCCTAGCGAGCTCGCTCATGCAGCCAACAGTGGCCTTGACATCGCTATTAGGCTATTGGAGCCTATCAAGGAACAGTTCCCGATCCTCACATATGCTGACTTTTACCAGTTGGCTGGTGTTGTTGCTGTCGAGATCACTGGAGGGCCTGAGGTTCCTTTCCACCCTGGTAGAGAGGACAAGCCCGCGCCACCACCGGAAGGTCGCTTGCCCGATGCTACCAAGGGTTCTGATCATTTGAGGGATGTTTTCTACACCATGGGCCTCTCTGACCAGGACATTGTCGCCCTCTCTGGCGGCCACACATTGGGTCGGGCACACAAGGAACGATCCGGGTTTGAGGGACCATGGACTTCCAACCCTCTCATCTTCGACAACTCGTATTTCACCGAGCTCTTGACCGGGGAGAAGGAAGGTCTTCTGCAGTTGGTATCGGACAAGACACTTCTATGCGACCCTGTCTTCCGCCCTCTCGTTGAAAAATATGCTGCAGATGAGGATGCTTTCTTTGCTGATTATGCTGAGGCTCACCAGAAGCTTTCTGAGCTCGGATTTGCTGATGCTTGAGCAATGGAAATGGAAAAATGAGGCTGCAGGCTGTTGTTGTTTATGTTAGCTTTGATTTGTAATGAAATGGGTAGGTTGGGTTGGGTTGGGTGGCCCTGTCTTTTTTTGTTGATTTGAATTTTGGATTTTGCCTCATCTCCCAGCCTCTTTGGACTTGAATAAAGTTGCTTCAAAGTTTATCATTTTGGTGTACTTTTTAGGCATAAACCAATCAATATCTTGAATGTTTTGAGCCCCAAATGCTTGCAAGTGGGTCAATTTGGC

Coding sequence (CDS)

ATGACAAAGTGCTACCCTGTTGTAAGTGAGGAATATCAAAAGGCCGTTGAAAAGGCCAAGAGGAAGCTTAGAGGACTCATTGCTGAGAAGAATTGTGCTCCTCTAATGCTCCGTCTTGCATGGCACTCTGCTGGAACCTTTGACCATAAATCCAAGACTGGTGGTCCGTTTGGAACTATGAGGTTCCCTAGCGAGCTCGCTCATGCAGCCAACAGTGGCCTTGACATCGCTATTAGGCTATTGGAGCCTATCAAGGAACAGTTCCCGATCCTCACATATGCTGACTTTTACCAGTTGGCTGGTGTTGTTGCTGTCGAGATCACTGGAGGGCCTGAGGTTCCTTTCCACCCTGGTAGAGAGGACAAGCCCGCGCCACCACCGGAAGGTCGCTTGCCCGATGCTACCAAGGGTTCTGATCATTTGAGGGATGTTTTCTACACCATGGGCCTCTCTGACCAGGACATTGTCGCCCTCTCTGGCGGCCACACATTGGGTCGGGCACACAAGGAACGATCCGGGTTTGAGGGACCATGGACTTCCAACCCTCTCATCTTCGACAACTCGTATTTCACCGAGCTCTTGACCGGGGAGAAGGAAGGTCTTCTGCAGTTGGTATCGGACAAGACACTTCTATGCGACCCTGTCTTCCGCCCTCTCGTTGAAAAATATGCTGCAGATGAGGATGCTTTCTTTGCTGATTATGCTGAGGCTCACCAGAAGCTTTCTGAGCTCGGATTTGCTGATGCTTGA

Protein sequence

MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTMRFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGREDKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTSNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQKLSELGFADA
Homology
BLAST of Carg12329 vs. NCBI nr
Match: XP_022948703.1 (L-ascorbate peroxidase, cytosolic-like [Cucurbita moschata] >KAG7036886.1 L-ascorbate peroxidase, cytosolic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.9 bits (1317), Expect = 3.1e-141
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM
Sbjct: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. NCBI nr
Match: KAG6607200.1 (L-ascorbate peroxidase, cytosolic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 511.9 bits (1317), Expect = 3.1e-141
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM
Sbjct: 40  MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 99

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 100 RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 159

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 160 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 219

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 220 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 279

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 280 LSELGFADA 288

BLAST of Carg12329 vs. NCBI nr
Match: XP_022997945.1 (L-ascorbate peroxidase, cytosolic-like [Cucurbita maxima])

HSP 1 Score: 510.8 bits (1314), Expect = 6.8e-141
Identity = 248/249 (99.60%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKS+TGGPFGTM
Sbjct: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. NCBI nr
Match: XP_023523165.1 (L-ascorbate peroxidase, cytosolic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.2e-139
Identity = 246/249 (98.80%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM
Sbjct: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLL+PIKEQFPILTYADFYQLAGVVAVEITGGP+VPFHPGRE
Sbjct: 61  RFPSELAHAANSGLDIAIRLLDPIKEQFPILTYADFYQLAGVVAVEITGGPDVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLL DPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLSDPVFRPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. NCBI nr
Match: XP_022159046.1 (L-ascorbate peroxidase, cytosolic [Momordica charantia])

HSP 1 Score: 485.3 bits (1248), Expect = 3.1e-133
Identity = 236/249 (94.78%), Postives = 241/249 (96.79%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M KCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFD KSKTGGPFGT+
Sbjct: 1   MGKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDKKSKTGGPFGTI 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +ELAH AN+GLDIA+RLLEPIKEQFPIL+YADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFSNELAHGANNGLDIAVRLLEPIKEQFPILSYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKP PPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPVPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH K
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKALLSDPVFRPLVEKYAADEDAFFADYAEAHLK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. ExPASy Swiss-Prot
Match: P48534 (L-ascorbate peroxidase, cytosolic OS=Pisum sativum OX=3888 GN=APX1 PE=1 SV=2)

HSP 1 Score: 441.4 bits (1134), Expect = 6.7e-123
Identity = 215/250 (86.00%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M K YP VS +YQKA+EKAKRKLRG IAEK CAPL+LRLAWHSAGTFD K+KTGGPFGT+
Sbjct: 1   MGKSYPTVSPDYQKAIEKAKRKLRGFIAEKKCAPLILRLAWHSAGTFDSKTKTGGPFGTI 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           +  +ELAH AN+GLDIA+RLLEPIKEQFPI++YADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  KHQAELAHGANNGLDIAVRLLEPIKEQFPIVSYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKGSDHLRDVF   MGLSDQDIVALSGGHT+G AHKERSGFEGPWT
Sbjct: 121 DKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYFTELLTGEK+GLLQL SDK LL D VFRPLVEKYAADED FFADYAEAH 
Sbjct: 181 SNPLIFDNSYFTELLTGEKDGLLQLPSDKALLTDSVFRPLVEKYAADEDVFFADYAEAHL 240

Query: 241 KLSELGFADA 250
           KLSELGFA+A
Sbjct: 241 KLSELGFAEA 250

BLAST of Carg12329 vs. ExPASy Swiss-Prot
Match: Q05431 (L-ascorbate peroxidase 1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX1 PE=1 SV=2)

HSP 1 Score: 431.0 bits (1107), Expect = 9.0e-120
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. ExPASy Swiss-Prot
Match: Q9FE01 (L-ascorbate peroxidase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=APX2 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.0e-118
Identity = 207/248 (83.47%), Postives = 225/248 (90.73%), Query Frame = 0

Query: 2   TKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTMR 61
           +K YP VS+EY  AV KAKRKLRGLIAEKNCAPLMLRLAWHSAGTFD  S+TGGPFGTM+
Sbjct: 3   SKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMK 62

Query: 62  FPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRED 121
            P E +HAAN+GLDIA+RLL+PIK+Q PIL+YADFYQLAGVVAVE+TGGPEVPFHPGR+D
Sbjct: 63  NPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQD 122

Query: 122 KPAPPPEGRLPDATKGSDHLRDVFYT-MGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 181
           KP PPPEGRLPDAT+GSDHLR VF   MGLSD+DIVALSGGHTLGR HKERSGFEG WTS
Sbjct: 123 KPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTS 182

Query: 182 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 241
           NPLIFDNSYFTEL++GEKEGLLQL SDK L+ DP FRPLVEKYAADEDAFFADYAEAH K
Sbjct: 183 NPLIFDNSYFTELVSGEKEGLLQLPSDKALMADPAFRPLVEKYAADEDAFFADYAEAHLK 242

Query: 242 LSELGFAD 249
           LSELGFA+
Sbjct: 243 LSELGFAE 250

BLAST of Carg12329 vs. ExPASy Swiss-Prot
Match: Q10N21 (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=APX1 PE=1 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.3e-118
Identity = 208/250 (83.20%), Postives = 225/250 (90.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M K YPVVS EYQ+AVEKA++KLR LIAEK+CAPLMLRLAWHSAGTFD  SKTGGPFGTM
Sbjct: 1   MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           + P+EL+HAAN+GLDIA+R+LEPIKE+ P ++YADFYQLAGVVAVE++GGP VPFHPGRE
Sbjct: 61  KTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKPAPPPEGRLPDATKGSDHLR VF   MGLSDQDIVALSGGHTLGR HKERSGFEGPWT
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
            NPL FDNSYFTELL+G+KEGLLQL SDK LL DP FRPLVEKYAADE AFF DY EAH 
Sbjct: 181 RNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFRPLVEKYAADEKAFFEDYKEAHL 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. ExPASy Swiss-Prot
Match: A2XFC7 (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=APX1 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-117
Identity = 207/250 (82.80%), Postives = 224/250 (89.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M K YPVVS EYQ+AVEKA++KLR LIAEK+CAPLMLRLAWHSAGTFD  SKTGGPFGTM
Sbjct: 1   MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           + P+EL+HAAN+GLDIA+R+LEPIKE+ P ++YADFYQLAGVVAVE++GGP VPFHPGRE
Sbjct: 61  KTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKPAPPPEGRLPDATKGSDHLR VF   MGLSDQDIVALSGGHTLGR HKERSGFEGPWT
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
            NPL FDNSYFTELL+G+KEGLLQL SDK LL DP F PLVEKYAADE AFF DY EAH 
Sbjct: 181 RNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFCPLVEKYAADEKAFFEDYKEAHL 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. ExPASy TrEMBL
Match: A0A6J1GA26 (L-ascorbate peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111452295 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.5e-141
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM
Sbjct: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. ExPASy TrEMBL
Match: A0A6J1KBB4 (L-ascorbate peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111492742 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 3.3e-141
Identity = 248/249 (99.60%), Postives = 249/249 (100.00%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKS+TGGPFGTM
Sbjct: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. ExPASy TrEMBL
Match: A0A6J1E2R0 (L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111025487 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.5e-133
Identity = 236/249 (94.78%), Postives = 241/249 (96.79%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M KCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFD KSKTGGPFGT+
Sbjct: 1   MGKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDKKSKTGGPFGTI 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +ELAH AN+GLDIA+RLLEPIKEQFPIL+YADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFSNELAHGANNGLDIAVRLLEPIKEQFPILSYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKP PPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPVPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH K
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKALLSDPVFRPLVEKYAADEDAFFADYAEAHLK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. ExPASy TrEMBL
Match: A0A4D6EYR9 (L-ascorbate peroxidase OS=Citrullus lanatus OX=3654 GN=CLAPX2 PE=2 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.6e-132
Identity = 230/249 (92.37%), Postives = 240/249 (96.39%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M KCYP VSEEYQKA+EKAKRKLRGLIAEKNCAPL+LRLAWHSAGTFDHK+KTGGPFGT+
Sbjct: 1   MVKCYPTVSEEYQKAIEKAKRKLRGLIAEKNCAPLILRLAWHSAGTFDHKTKTGGPFGTI 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF SELAH AN+GLDIA+RLLEPIK+QFPIL+YADFYQLAGVVAVE+TGGPEVPFHPGRE
Sbjct: 61  RFSSELAHGANNGLDIAVRLLEPIKQQFPILSYADFYQLAGVVAVEVTGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKP PPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPEPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELL+GEKEGLLQL SDK LL DPVF PLVEKYAADEDAFFADYAEAHQK
Sbjct: 181 NPLIFDNSYFTELLSGEKEGLLQLASDKALLSDPVFHPLVEKYAADEDAFFADYAEAHQK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. ExPASy TrEMBL
Match: A0A068JA92 (L-ascorbate peroxidase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.1e-132
Identity = 235/249 (94.38%), Postives = 240/249 (96.39%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           M KCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFD KSKTGGPFGT+
Sbjct: 1   MGKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDKKSKTGGPFGTI 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +ELAH AN+GLDIA+RLLEPIKEQFPIL+YADFYQLAGVVAVEITGGPEVPFHPGRE
Sbjct: 61  RFSNELAHGANNGLDIAVRLLEPIKEQFPILSYADFYQLAGVVAVEITGGPEVPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180
           DKP P PEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS
Sbjct: 121 DKPVPLPEGRLPDATKGSDHLRDVFYTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWTS 180

Query: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQK 240
           NPLIFDNSYFTELLTGEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH K
Sbjct: 181 NPLIFDNSYFTELLTGEKEGLLQLVSDKALLSDPVFRPLVEKYAADEDAFFADYAEAHLK 240

Query: 241 LSELGFADA 250
           LSELGFADA
Sbjct: 241 LSELGFADA 249

BLAST of Carg12329 vs. TAIR 10
Match: AT1G07890.3 (ascorbate peroxidase 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 6.4e-121
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. TAIR 10
Match: AT1G07890.1 (ascorbate peroxidase 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 6.4e-121
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. TAIR 10
Match: AT1G07890.2 (ascorbate peroxidase 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 6.4e-121
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. TAIR 10
Match: AT1G07890.4 (ascorbate peroxidase 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 6.4e-121
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

BLAST of Carg12329 vs. TAIR 10
Match: AT1G07890.5 (ascorbate peroxidase 1 )

HSP 1 Score: 431.0 bits (1107), Expect = 6.4e-121
Identity = 207/250 (82.80%), Postives = 229/250 (91.60%), Query Frame = 0

Query: 1   MTKCYPVVSEEYQKAVEKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDHKSKTGGPFGTM 60
           MTK YP VSE+Y+KAVEK +RKLRGLIAEKNCAP+M+RLAWHSAGTFD +S+TGGPFGTM
Sbjct: 1   MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60

Query: 61  RFPSELAHAANSGLDIAIRLLEPIKEQFPILTYADFYQLAGVVAVEITGGPEVPFHPGRE 120
           RF +E AH ANSG+ IA+RLL+PI+EQFP +++ADF+QLAGVVAVE+TGGP++PFHPGRE
Sbjct: 61  RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120

Query: 121 DKPAPPPEGRLPDATKGSDHLRDVF-YTMGLSDQDIVALSGGHTLGRAHKERSGFEGPWT 180
           DKP PPPEGRLPDATKG DHLRDVF   MGLSD+DIVALSG HTLGR HK+RSGFEG WT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180

Query: 181 SNPLIFDNSYFTELLTGEKEGLLQLVSDKTLLCDPVFRPLVEKYAADEDAFFADYAEAHQ 240
           SNPLIFDNSYF ELL+GEKEGLLQLVSDK LL DPVFRPLVEKYAADEDAFFADYAEAH 
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240

Query: 241 KLSELGFADA 250
           KLSELGFADA
Sbjct: 241 KLSELGFADA 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948703.13.1e-141100.00L-ascorbate peroxidase, cytosolic-like [Cucurbita moschata] >KAG7036886.1 L-asco... [more]
KAG6607200.13.1e-141100.00L-ascorbate peroxidase, cytosolic, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022997945.16.8e-14199.60L-ascorbate peroxidase, cytosolic-like [Cucurbita maxima][more]
XP_023523165.12.2e-13998.80L-ascorbate peroxidase, cytosolic-like [Cucurbita pepo subsp. pepo][more]
XP_022159046.13.1e-13394.78L-ascorbate peroxidase, cytosolic [Momordica charantia][more]
Match NameE-valueIdentityDescription
P485346.7e-12386.00L-ascorbate peroxidase, cytosolic OS=Pisum sativum OX=3888 GN=APX1 PE=1 SV=2[more]
Q054319.0e-12082.80L-ascorbate peroxidase 1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX1 PE=1... [more]
Q9FE011.0e-11883.47L-ascorbate peroxidase 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q10N211.3e-11883.20L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
A2XFC71.1e-11782.80L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. indica OX=39946 GN=AP... [more]
Match NameE-valueIdentityDescription
A0A6J1GA261.5e-141100.00L-ascorbate peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111452295 PE=3 SV=1[more]
A0A6J1KBB43.3e-14199.60L-ascorbate peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111492742 PE=3 SV=1[more]
A0A6J1E2R01.5e-13394.78L-ascorbate peroxidase OS=Momordica charantia OX=3673 GN=LOC111025487 PE=3 SV=1[more]
A0A4D6EYR91.6e-13292.37L-ascorbate peroxidase OS=Citrullus lanatus OX=3654 GN=CLAPX2 PE=2 SV=1[more]
A0A068JA922.1e-13294.38L-ascorbate peroxidase OS=Momordica charantia OX=3673 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G07890.36.4e-12182.80ascorbate peroxidase 1 [more]
AT1G07890.16.4e-12182.80ascorbate peroxidase 1 [more]
AT1G07890.26.4e-12182.80ascorbate peroxidase 1 [more]
AT1G07890.46.4e-12182.80ascorbate peroxidase 1 [more]
AT1G07890.56.4e-12182.80ascorbate peroxidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 9..29
NoneNo IPR availableGENE3D1.10.420.10Peroxidase, domain 2coord: 132..238
e-value: 3.8E-109
score: 365.3
NoneNo IPR availableGENE3D1.10.520.10coord: 19..245
e-value: 3.8E-109
score: 365.3
NoneNo IPR availablePIRSRPIRSR600823-3PIRSR600823-3coord: 92..168
e-value: 2.5E-11
score: 40.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..136
NoneNo IPR availablePANTHERPTHR31356:SF45L-ASCORBATE PEROXIDASE 1, CYTOSOLICcoord: 1..249
NoneNo IPR availableCDDcd00691ascorbate_peroxidasecoord: 5..249
e-value: 8.38723E-157
score: 433.553
IPR002016Haem peroxidasePRINTSPR00458PEROXIDASEcoord: 91..108
score: 36.09
coord: 178..193
score: 44.74
coord: 109..121
score: 48.11
coord: 33..47
score: 41.14
coord: 154..169
score: 56.5
IPR002016Haem peroxidasePFAMPF00141peroxidasecoord: 22..226
e-value: 3.8E-44
score: 151.1
IPR002016Haem peroxidasePROSITEPS50873PEROXIDASE_4coord: 90..249
score: 16.65613
IPR002207Class I peroxidasePRINTSPR00459ASPEROXIDASEcoord: 51..61
score: 79.37
coord: 12..32
score: 76.37
coord: 222..246
score: 87.69
coord: 33..48
score: 87.74
coord: 90..108
score: 80.77
coord: 109..121
score: 84.62
coord: 170..194
score: 86.15
coord: 148..169
score: 88.99
coord: 130..147
score: 75.64
coord: 65..89
score: 70.62
IPR044831Heme-binding peroxidase Ccp1-likePANTHERPTHR31356THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATEDcoord: 1..249
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 154..164
IPR019794Peroxidase, active sitePROSITEPS00436PEROXIDASE_2coord: 33..44
IPR010255Haem peroxidase superfamilySUPERFAMILY48113Heme-dependent peroxidasescoord: 5..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12329-RACarg12329-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0034599 cellular response to oxidative stress
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0009507 chloroplast
molecular_function GO:0020037 heme binding
molecular_function GO:0016688 L-ascorbate peroxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004601 peroxidase activity