Carg12324 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12324
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like
LocationCarg_Chr01: 2949110 .. 2953355 (-)
RNA-Seq ExpressionCarg12324
SyntenyCarg12324
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTGCGGACTTTCCTTCCCAATCTTCGGTAATGGTGGCGTTCAAACAGTTCTTTTCTACGCCATTTTCACCTTTTTTTGCGTTTCCATTACTGGTTGAAATTGTTGACCCAATATCATCAATCGAATAGCTACAATCTCGAGTTATAGGTATGTATTTTGAACTTGATTTCACTTTGGATTTGCTGTAATTTTCTGGATTTCGTTGTTTGTACGAATCCTCATTTCGTCTTTTTACATCTGGATTTGATGATTTGATTGAGGATGCCATTGATGTTTCTTATGGATTTCTGTCTTCTCTTGCGTACAGTGATCATAATAAGTAGAGAAAGCGTATTACTATTTTTTATCATCTTTGGAATTTGGAAACTAAGTAATATGATTGTTAGATGCTGCGGGAGAGTGAATTGAATGTCCTATTTCTTTCACAAAAACAAGAGGAACACTAAATTCTTCTAAATATCTATGTTTTATTTTGAACAACAATCGTACTTGAAGGAAATTCTAAAACTAAGAGGTGAATATCATTTTGGGAGATGACTTTGGTAAGGAAAACTTTATGTAGTTTTGAATTGGCGAAAAGTTTACGGGTGTCATTCTTGATCATTGAACTGAACATGAACTTGTATCTATTTCCAGTCATTTTGATGATGAAAACGAATAGGTATTTCTTGAAGTTGTTGTATTATCTGCTGATAACTTCAAAATTTGTTACTTCCACAGGCTGTTTTCTAAATTTTGTTTCACTGTTGCTGTTGTGCAAAAATGGTGGTGGATACTCATGCGCAGGTTGCAAAATTTTCTGTGGCCTTACCCTCGTCCTTGGTTACCCTTCCACATAGATCTTCAAATAGGTTGTCTTATCATCTCCCATTGGGTCTAAAAGCAAAAGTAAAAAAGATAAAGTCCTCATCTGCTACTGATGCAGGACATAGTGTGCCTCCCTCATCTTCAGAAAGAAGGAATCCACTTGCCATTTTTCTTGATGTTCCTCGTACTGTATGGAGGCAAACATTGCGTCCATTGAGTAATTTTGGGTTCGGTCAAAGGAGCATATGGGAAGGTGGGGTTGGGTTGTTTCTAGTGTCAGGTGCTATACTTCTCACACTCAGTTTGGCTTGGTTGAGAGGCTTTCAACTGCGGTCTAAATTTAGAAAATACTTGGCTGTCTTTGAGTTTGCTCAGGCCTGTGGTATTTCTACTGGAACTCCTGTAAGAATTAGAGGGGTTACTGTTGGCAATGTCATTCGTATTAACCCTTCCTTGAGATGTATTGAAACTGTTGTTGAGGTATGCAAATTCATATCCTTTTTATCATTCTATCCTTGATTATCTAACCAAAGACTAAAAATACTTTTTGTGTCTGAAATTACTTTTTATTCTTCAAAATCATCTCAAACTCATGTAAGTTTATCGTTAGTTTGAGAACCAAAAGTTAAACTATATAGGTGTGGGATCTCACATCGGTTGGAGAGAGGAACGAAGTATTCCTTATAAGGGTATGGAAACCTCTTCCTAGCAAACACGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGAGTAAGCTCAATGAGGATAATATCTGCTGGCGGCTAGCATTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACATCGGGCGGTGTGCTAGCGAGGACGTCGGGTCCCCAAGAGGGATGGATTGTGAGATCCCACATCGGTTGGAGAGAGGAATGAAGTATTCCATATGAGGGTATGGAAACCTCTCCCTGGCAGACATGTTTTAAAAACCTTGAGAGAAAACTCGAAAGGAAAAACCCAAAAGGGGACAATATCTGCTAGCGGTGGGCTTGGGTTGTTACAATAGGCTAGTTAACAAATAGGCTTACTCTTTGACTTACAAAGATTAAAAAAAGAAAAAGAAAAAGAAAAGGAGAAGACATTGACTAAGATGCGTAAAAGGAAAGAAAATAAAAAAGAGAGACGTTTTAGTTTCCTTAATCAAAGCTTTTAACAAAGAGTTTGTACCATATGCCATTTAGTTCATATTATCTTCCTATAGAGTTCATCATAGTTATTTTCCATTGGCATGAATTCGTTTTCTTACCATTTTTTTTTCTATCACAAAATGAAATATACCACCCATTTTGCAGCTTGAAGATGATAAGATAATTGTACCACGTAATTCATTGGTTGAAGTAAACCAGTCTGGTCTTCTTATGGAGACAGTGATTGACATTACTCCTCGCAATCCTATTCCAGTGCCTTCAGCGGGACCACTTGACTCTGAATGTATCAAAGAAGGTTTAATATTATGCGATAAGCAGAAAATGAAGGGGCATCAAGGGGTAAGTTTGGATGAATTAGTTGGAATATTCACTCGGCTTGGGCGCGAAGCGGAGGAAATAGGGCTTTCCAATACATTTGCATTAGCTCAACGAGTTGCTTTGGTTATTGAAGAAGCAAAGCCTTTGCTTTTAAAGGTCCGACTCCTTACAATTTCATGCTCTAAACTTTATTTTCTAGCTTGGTTCCTAGGCAGTCATTCAAATTATGAATGTTCATATCTTGTAAATATTATAAGAAAATATTTTGTTGGCAACTCTGATGGGATGTTCACTGATTTCATGAAGAAGATGGAAGGCTTGTAAAAAAACAATAAATACTATGTATTCGTATGCCAGCACTTCCATGGAATCTGGTTACTTATTAAGGCTTCTAGATTTACCTAGTAGGTAGGATCTGGACTTTTGCTAAAAAAAACTTTTAAAGAGCAAATATGATTGGAACATTGATAGCTCAAACATAGGAGTTGCATTGCGGCTAGAAACTGGGAGTTTTCTTGGGTATTCCGGTATGAGCCTTCGAACGGAATCAGGAATTTATAGCTTTAATCCTTGAAAGTTGTAATTATTTGTGTTAAAATGTATTAGGTTCTTGTTTGATTGTCTATTTTGATGAGTGAGATATATAGTTCCACGTATCTAACTATCTTCCTATGAAAAATGTTTGTTTTGATGTAGTGATGTCTCAGATTCAAGCCATGGCTGAAGATGTTCAACCTTTGCTCGCTGAGCTTCGTGACAGTGGTCTTCTAAAGGAGGTTGAAAATTTAACTAAAAGTTTGTCACATGCCACAGAAGATTTAAGGTAAACTATTCCTGAAGTCTGAAATTGGAATTTTAATGTAGATAAGCTCTGATTTCTGAATTGCCACAAACTTTATAATGAAGCTTCCCCGTTGAGGAAATGAAAAGGGTCTATAATTTTCAATCCTTTGACAACTTTCACCAGAAATTAGTCGCTCTTTTCTTGATTCTTTTATGCATTTCTTCAAGAGAACAGCCTAAATAACATTTGTACAACAGATTTTGGCCTTTTCCTTGCACAAAAATCAGCTCAAACCCAAAACATACATGATGATTTTGAAAAAGAAAAAGAAGAATCAATCTTTGAATTCCTTTTACATGATGTATGCTTATTGAGACTTTTTCGATCGTTTACAGAAGCGTGCATGCGTCCATTATGACTCCCGAAAACACAGCACTGCTTCAGAAATCCATTTATACGCTAATTCTTACTCTGAAGAACATAGAGGTAAGATCCTAACTGGTCGAGTTCTTTCCGGTTTGCCCCAAGCAGTTACTTCTCATAATTACTTTCTTTACCGTTCTAACTCCTTGTTCCTCACTATTGCAGAGTTTGAGCTCAGAACTTCTTGGGTTCACTGGCGATGAGGCTACAAAACGGAATTTAAAAGTGCTGATCAAGTCTCTCAGCAGGCTGCTATGAAACAAAGATATGAACTTGGTATTAGACAACATTGTAGCATCTCAACCTTTTCATATTGCGAGCACAAAGCCGAAGTGGCTCGACGTAGTTTCATAACTGTTATGGTTTCGGCTTCCGCAGGGTGGGGAGGGAGGGAGTCAGGCTCAGGCATTGGCAAAATTTTTGAGATATTTGTGTATGTTCTTAGGTAGTAGCCCTGCTTTAACAGAAGGAAACTGTTGGTTCTTATAACACATATTGGTCCATTTCTTTCAAGGCTTCTTTCTTGTAGGCTGTGGCTTTGGCTGATTTCATAGACATTTGAATGGGTAGGGGCATACTTGCAAGTTGATTGGTCCCAAGGGTACTAGAGAAAAATGGGAGGCATAAAGCAAATGACCCTTCGACGTAGGGATGTTCGTGAGTTGGATTAGGTCATATTGGAACCCGAGTCTACTAATTAGATTGAATTTATAACCATAGTCTTTTAAATCAATA

mRNA sequence

TCTGCGGACTTTCCTTCCCAATCTTCGGTAATGGTGGCGTTCAAACAGTTCTTTTCTACGCCATTTTCACCTTTTTTTGCGTTTCCATTACTGGTTGAAATTGTTGACCCAATATCATCAATCGAATAGCTACAATCTCGAGTTATAGGTATGTATTTTGAACTTGATTTCACTTTGGATTTGCTGTAATTTTCTGGATTTCGTTGTTTGTACGAATCCTCATTTCGTCTTTTTACATCTGGATTTGATGATTTGATTGAGGATGCCATTGATGTTTCTTATGGATTTCTGTCTTCTCTTGCGTACAGTGATCATAATAAGTAGAGAAAGCGTATTACTATTTTTTATCATCTTTGGAATTTGGAAACTAAGTAATATGATTGTTAGATGCTGCGGGAGAGTGAATTGAATGTCCTATTTCTTTCACAAAAACAAGAGGAACACTAAATTCTTCTAAATATCTATGTTTTATTTTGAACAACAATCGTACTTGAAGGAAATTCTAAAACTAAGAGGTGAATATCATTTTGGGAGATGACTTTGGTAAGGAAAACTTTATGTAGTTTTGAATTGGCGAAAAGTTTACGGGTGTCATTCTTGATCATTGAACTGAACATGAACTTGTATCTATTTCCAGTCATTTTGATGATGAAAACGAATAGGTATTTCTTGAAGTTGTTGTATTATCTGCTGATAACTTCAAAATTTGTTACTTCCACAGGCTGTTTTCTAAATTTTGTTTCACTGTTGCTGTTGTGCAAAAATGGTGGTGGATACTCATGCGCAGGTTGCAAAATTTTCTGTGGCCTTACCCTCGTCCTTGGTTACCCTTCCACATAGATCTTCAAATAGGTTGTCTTATCATCTCCCATTGGGTCTAAAAGCAAAAGTAAAAAAGATAAAGTCCTCATCTGCTACTGATGCAGGACATAGTGTGCCTCCCTCATCTTCAGAAAGAAGGAATCCACTTGCCATTTTTCTTGATGTTCCTCGTACTGTATGGAGGCAAACATTGCGTCCATTGAGTAATTTTGGGTTCGGTCAAAGGAGCATATGGGAAGGTGGGGTTGGGTTGTTTCTAGTGTCAGGTGCTATACTTCTCACACTCAGTTTGGCTTGGTTGAGAGGCTTTCAACTGCGGTCTAAATTTAGAAAATACTTGGCTGTCTTTGAGTTTGCTCAGGCCTGTGGTATTTCTACTGGAACTCCTGTAAGAATTAGAGGGGTTACTGTTGGCAATGTCATTCGTATTAACCCTTCCTTGAGATGTATTGAAACTGTTGTTGAGCTTGAAGATGATAAGATAATTGTACCACGTAATTCATTGGTTGAAGTAAACCAGTCTGGTCTTCTTATGGAGACAGTGATTGACATTACTCCTCGCAATCCTATTCCAGTGCCTTCAGCGGGACCACTTGACTCTGAATGTATCAAAGAAGGTTTAATATTATGCGATAAGCAGAAAATGAAGGGGCATCAAGGGGTAAGTTTGGATGAATTAGTTGGAATATTCACTCGGCTTGGGCGCGAAGCGGAGGAAATAGGGCTTTCCAATACATTTGCATTAGCTCAACGAGTTGCTTTGGTTATTGAAGAAGCAAAGCCTTTGCTTTTAAAGATTCAAGCCATGGCTGAAGATGTTCAACCTTTGCTCGCTGAGCTTCGTGACAGTGGTCTTCTAAAGGAGGTTGAAAATTTAACTAAAAGTTTGTCACATGCCACAGAAGATTTAAGAAGCGTGCATGCGTCCATTATGACTCCCGAAAACACAGCACTGCTTCAGAAATCCATTTATACGCTAATTCTTACTCTGAAGAACATAGAGAGTTTGAGCTCAGAACTTCTTGGGTTCACTGGCGATGAGGCTACAAAACGGAATTTAAAAGTGCTGATCAAGTCTCTCAGCAGGCTGCTATGAAACAAAGATATGAACTTGGTATTAGACAACATTGTAGCATCTCAACCTTTTCATATTGCGAGCACAAAGCCGAAGTGGCTCGACGTAGTTTCATAACTGTTATGGTTTCGGCTTCCGCAGGGTGGGGAGGGAGGGAGTCAGGCTCAGGCATTGGCAAAATTTTTGAGATATTTGTGTATGTTCTTAGGTAGTAGCCCTGCTTTAACAGAAGGAAACTGTTGGTTCTTATAACACATATTGGTCCATTTCTTTCAAGGCTTCTTTCTTGTAGGCTGTGGCTTTGGCTGATTTCATAGACATTTGAATGGGTAGGGGCATACTTGCAAGTTGATTGGTCCCAAGGGTACTAGAGAAAAATGGGAGGCATAAAGCAAATGACCCTTCGACGTAGGGATGTTCGTGAGTTGGATTAGGTCATATTGGAACCCGAGTCTACTAATTAGATTGAATTTATAACCATAGTCTTTTAAATCAATA

Coding sequence (CDS)

ATGGTGGTGGATACTCATGCGCAGGTTGCAAAATTTTCTGTGGCCTTACCCTCGTCCTTGGTTACCCTTCCACATAGATCTTCAAATAGGTTGTCTTATCATCTCCCATTGGGTCTAAAAGCAAAAGTAAAAAAGATAAAGTCCTCATCTGCTACTGATGCAGGACATAGTGTGCCTCCCTCATCTTCAGAAAGAAGGAATCCACTTGCCATTTTTCTTGATGTTCCTCGTACTGTATGGAGGCAAACATTGCGTCCATTGAGTAATTTTGGGTTCGGTCAAAGGAGCATATGGGAAGGTGGGGTTGGGTTGTTTCTAGTGTCAGGTGCTATACTTCTCACACTCAGTTTGGCTTGGTTGAGAGGCTTTCAACTGCGGTCTAAATTTAGAAAATACTTGGCTGTCTTTGAGTTTGCTCAGGCCTGTGGTATTTCTACTGGAACTCCTGTAAGAATTAGAGGGGTTACTGTTGGCAATGTCATTCGTATTAACCCTTCCTTGAGATGTATTGAAACTGTTGTTGAGCTTGAAGATGATAAGATAATTGTACCACGTAATTCATTGGTTGAAGTAAACCAGTCTGGTCTTCTTATGGAGACAGTGATTGACATTACTCCTCGCAATCCTATTCCAGTGCCTTCAGCGGGACCACTTGACTCTGAATGTATCAAAGAAGGTTTAATATTATGCGATAAGCAGAAAATGAAGGGGCATCAAGGGGTAAGTTTGGATGAATTAGTTGGAATATTCACTCGGCTTGGGCGCGAAGCGGAGGAAATAGGGCTTTCCAATACATTTGCATTAGCTCAACGAGTTGCTTTGGTTATTGAAGAAGCAAAGCCTTTGCTTTTAAAGATTCAAGCCATGGCTGAAGATGTTCAACCTTTGCTCGCTGAGCTTCGTGACAGTGGTCTTCTAAAGGAGGTTGAAAATTTAACTAAAAGTTTGTCACATGCCACAGAAGATTTAAGAAGCGTGCATGCGTCCATTATGACTCCCGAAAACACAGCACTGCTTCAGAAATCCATTTATACGCTAATTCTTACTCTGAAGAACATAGAGAGTTTGAGCTCAGAACTTCTTGGGTTCACTGGCGATGAGGCTACAAAACGGAATTTAAAAGTGCTGATCAAGTCTCTCAGCAGGCTGCTATGA

Protein sequence

MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPPSSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWLRGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQGVSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAELRDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSELLGFTGDEATKRNLKVLIKSLSRLL
Homology
BLAST of Carg12324 vs. NCBI nr
Match: XP_022948293.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita moschata] >XP_022948294.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita moschata] >KAG7036881.1 Protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 729.6 bits (1882), Expect = 1.4e-206
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP
Sbjct: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG
Sbjct: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL
Sbjct: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLKVLIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKVLIKSLSRLL 384

BLAST of Carg12324 vs. NCBI nr
Match: XP_023523118.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023523123.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.1 bits (1847), Expect = 1.6e-202
Identity = 378/384 (98.44%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MVVDTH QVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLK KVKKI+SSSA DAGHSVPP
Sbjct: 1   MVVDTHVQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKTKVKKIRSSSA-DAGHSVPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG
Sbjct: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQP LAEL
Sbjct: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPFLAEL 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLKVLIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKVLIKSLSRLL 383

BLAST of Carg12324 vs. NCBI nr
Match: XP_022998942.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita maxima] >XP_022998943.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 1.7e-199
Identity = 372/384 (96.88%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           M+ DTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLK KVKKI+SSSA DAGHSVPP
Sbjct: 1   MMGDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKTKVKKIRSSSAADAGHSVPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGF QRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFCQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           IIVPRNSLVEVNQSGLLME VIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG
Sbjct: 181 IIVPRNSLVEVNQSGLLMEIVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLL KIQAMAEDVQPLLAEL
Sbjct: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLFKIQAMAEDVQPLLAEL 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           R+SGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL
Sbjct: 301 RESGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEA K NLKVLIKSLSRLL
Sbjct: 361 LGFTGDEAIKPNLKVLIKSLSRLL 384

BLAST of Carg12324 vs. NCBI nr
Match: XP_023554510.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554511.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554512.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 673.7 bits (1737), Expect = 9.4e-190
Identity = 352/384 (91.67%), Postives = 370/384 (96.35%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MV D   QVA FSVALPSSLVTLPHRSS+RLSYHLP GLK+KVKKIK++SA DAGHS PP
Sbjct: 1   MVGDARVQVAAFSVALPSSLVTLPHRSSSRLSYHLPFGLKSKVKKIKATSA-DAGHSQPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLA+FLDVPRTVWR+TLRPLSNFGFG+RSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLALFLDVPRTVWRRTLRPLSNFGFGRRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIR+NPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRVNPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           II+P NSLVEVNQSGLLMET+IDITPR+PIPVPSAGPLDSECIKEGLILCDK KMKGHQG
Sbjct: 181 IIIPLNSLVEVNQSGLLMETMIDITPRDPIPVPSAGPLDSECIKEGLILCDKHKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLD LVGIFTRLGREAEEIGL+NTF+LAQRVALV+EEA+PLLLKIQAMAEDVQPLLAE+
Sbjct: 241 VSLDALVGIFTRLGREAEEIGLTNTFSLAQRVALVVEEARPLLLKIQAMAEDVQPLLAEV 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLT SLSHATEDLRSVHASIMTPENT LLQKSIYTLI TLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTSSLSHATEDLRSVHASIMTPENTELLQKSIYTLIHTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLK+LIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKLLIKSLSRLL 383

BLAST of Carg12324 vs. NCBI nr
Match: XP_022952170.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022952171.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_022952172.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_022952173.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022952174.1 protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 668.3 bits (1723), Expect = 3.9e-188
Identity = 348/384 (90.62%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MV D   QVA FSVALPSSLVTLPH+SS+RLSYHLP GLK+KVKKIK++SA DAGHS PP
Sbjct: 1   MVGDARVQVAAFSVALPSSLVTLPHKSSSRLSYHLPFGLKSKVKKIKATSA-DAGHSQPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSER NPLA+FLDVPRTVWR+TLRPLSNFGFG+RSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERTNPLALFLDVPRTVWRRTLRPLSNFGFGRRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIR+NPSLRCIET+VE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRVNPSLRCIETIVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           II+P NSLVEVNQSGLLMET+IDITPR+PIPVPS GPLDSECIKEGLILCDK KMKGHQG
Sbjct: 181 IIIPLNSLVEVNQSGLLMETMIDITPRDPIPVPSVGPLDSECIKEGLILCDKHKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLD LVGIFTRLGREAEEIGL+NTF+LAQRVALV+EEA+PLLLKIQAMAEDVQPLLAE+
Sbjct: 241 VSLDALVGIFTRLGREAEEIGLTNTFSLAQRVALVVEEARPLLLKIQAMAEDVQPLLAEV 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLT SLSHATEDLRSVHASIMTPENT LLQKSIYTLI TLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTSSLSHATEDLRSVHASIMTPENTELLQKSIYTLIHTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLK+LIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKLLIKSLSRLL 383

BLAST of Carg12324 vs. ExPASy Swiss-Prot
Match: Q9LTR2 (Protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TGD2 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 5.4e-140
Identity = 257/367 (70.03%), Postives = 308/367 (83.92%), Query Frame = 0

Query: 18  SSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPPSSSERRNPLAIFLDVPR 77
           SS++  P  S N + Y LP   + +   ++++S +DA H   PSS   +NPL + LDVPR
Sbjct: 17  SSMIACPRVSPNGVPY-LPPKPRTRHLVVRAASNSDAAHG-QPSSDGGKNPLTVVLDVPR 76

Query: 78  TVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWLRGFQLRSKFRKYLAVFE 137
            +WRQTL+PLS+FGFG+RSIWEGGVGLF+VSGA LL LS AWLRGFQ+RSKFRKY  VFE
Sbjct: 77  NIWRQTLKPLSDFGFGKRSIWEGGVGLFIVSGATLLALSWAWLRGFQMRSKFRKYQTVFE 136

Query: 138 FAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVPRNSLVEVNQSGLL 197
            + A GI TGTPVRIRGVTVG +IR+NPSL+ IE V E+EDDKII+PRNSLVEVNQSGLL
Sbjct: 137 LSHASGICTGTPVRIRGVTVGTIIRVNPSLKNIEAVAEIEDDKIIIPRNSLVEVNQSGLL 196

Query: 198 METVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQGVSLDELVGIFTRLGREA 257
           MET+IDI PRNPIP PS GPL  EC KEGLI+CD+Q +KG QGVSLDELVGIFTR+GRE 
Sbjct: 197 METMIDIMPRNPIPEPSVGPLHPECGKEGLIVCDRQTIKGVQGVSLDELVGIFTRIGREV 256

Query: 258 EEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAELRDSGLLKEVENLTKSLS 317
           E IG++NT++LA+R A VIEEA+PLL KIQAMAED QPLL+E RDSGLLKEVE LT+SL+
Sbjct: 257 EAIGVANTYSLAERAASVIEEARPLLKKIQAMAEDAQPLLSEFRDSGLLKEVECLTRSLT 316

Query: 318 HATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSELLGFTGDEATKRNLKVLI 377
            A++DLR V++SIMTPENT L+QKSIYTL+ TLKN+ES+SS++LGFTGDEAT++NLK+LI
Sbjct: 317 QASDDLRKVNSSIMTPENTELIQKSIYTLVYTLKNVESISSDILGFTGDEATRKNLKLLI 376

Query: 378 KSLSRLL 385
           KSLSRLL
Sbjct: 377 KSLSRLL 381

BLAST of Carg12324 vs. ExPASy Swiss-Prot
Match: Q1XDB5 (Uncharacterized protein ycf22 OS=Pyropia yezoensis OX=2788 GN=ycf22 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-19
Identity = 58/162 (35.80%), Postives = 89/162 (54.94%), Query Frame = 0

Query: 102 VGLFLVSGAILLTLSLAWLRGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVI 161
           +G   +    LL++SL W        K   Y A  EF  A GI  GT VR+RG+ VG V+
Sbjct: 13  LGACFLGSVTLLSISL-W-NIINQAGKNHSYKAFIEFDSAYGIQEGTAVRLRGLPVGKVV 72

Query: 162 RINPSLRCIETVVELEDDKIIVPRNSLVEVNQSGLLMETVIDITPRNPIPVP----SAGP 221
            I+ S   I T +E++    I+P+ SL+E NQ+GLL +TVIDI P + + +      AGP
Sbjct: 73  GISQSSNSILTSIEIKSSSTIIPKTSLIETNQTGLLNDTVIDIIPLSKLSIDYSSIKAGP 132

Query: 222 LDSECIKEGLILCDKQKMKGHQGVSLDELVGIFTRLGREAEE 260
           L   C     I+C+   +KG +G++ D+L+   TR+ +  ++
Sbjct: 133 LSGAC-DNSQIICNLNYLKGERGLNYDDLIRATTRISQRFDD 171

BLAST of Carg12324 vs. ExPASy Swiss-Prot
Match: P51372 (Uncharacterized protein ycf22 OS=Porphyra purpurea OX=2787 GN=ycf22 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.6e-17
Identity = 48/137 (35.04%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 127 SKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVPRN 186
           SK + Y    EF  A GI  GT VR+RG+ +G V+ I+ S   I T +E++    I+P+ 
Sbjct: 36  SKNKSYKVFVEFDSAYGIQEGTSVRLRGLPIGKVVGISQSSHSILTRIEIQSCNTIIPKT 95

Query: 187 SLVEVNQSGLLMETVIDITPRNPIPVP----SAGPLDSECIKEGLILCDKQKMKGHQGVS 246
           SL+E NQ+GLL +T+IDI P   +         GPL   C     I+C    ++G +G++
Sbjct: 96  SLIETNQTGLLNDTIIDIVPFTTLNQEYHSLKEGPLSKTC-DSNQIICHLNYLQGERGLN 155

Query: 247 LDELVGIFTRLGREAEE 260
            D+L+   TR+ +  ++
Sbjct: 156 YDDLIRATTRISQRFDD 171

BLAST of Carg12324 vs. ExPASy Swiss-Prot
Match: P46315 (Uncharacterized protein ycf22 OS=Antithamnion sp. OX=2767 GN=ycf22 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.7e-13
Identity = 43/147 (29.25%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 128 KFRK---YLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVP 187
           K++K   Y    EF  A GI  GT V +RGV +G +  +  +   +   + ++ +KI++P
Sbjct: 15  KYKKKHGYSLFVEFTHAYGIGEGTSVNMRGVNIGYIKNLQINSNSVLVSIYIKSEKILIP 74

Query: 188 RNSLVEVNQSGLLMETVIDITPR---NPIPVPSAGPLDSECIKEGLILCDKQKMKGHQGV 247
           +NS++E NQ+ L   T+IDI P    N   +      +  C  +  I C+ Q + G +G+
Sbjct: 75  KNSIIETNQTSLFNNTIIDIIPLEKINNYSIRDFNVFNQNCY-DLQIFCNNQYIIGDRGL 134

Query: 248 SLDELVGIFTRLGREAEEIGLSNTFAL 269
           + D+L+   TR+ +  ++    N F L
Sbjct: 135 NYDDLIRATTRIAQRFDDPRFFNLFYL 160

BLAST of Carg12324 vs. ExPASy TrEMBL
Match: A0A6J1G9G9 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452008 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.0e-207
Identity = 384/384 (100.00%), Postives = 384/384 (100.00%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP
Sbjct: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG
Sbjct: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL
Sbjct: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLKVLIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKVLIKSLSRLL 384

BLAST of Carg12324 vs. ExPASy TrEMBL
Match: A0A6J1K9F4 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493450 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 8.3e-200
Identity = 372/384 (96.88%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           M+ DTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLK KVKKI+SSSA DAGHSVPP
Sbjct: 1   MMGDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKTKVKKIRSSSAADAGHSVPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGF QRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFCQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           IIVPRNSLVEVNQSGLLME VIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG
Sbjct: 181 IIVPRNSLVEVNQSGLLMEIVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLL KIQAMAEDVQPLLAEL
Sbjct: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLFKIQAMAEDVQPLLAEL 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           R+SGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL
Sbjct: 301 RESGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEA K NLKVLIKSLSRLL
Sbjct: 361 LGFTGDEAIKPNLKVLIKSLSRLL 384

BLAST of Carg12324 vs. ExPASy TrEMBL
Match: A0A6J1GJI3 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454927 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.9e-188
Identity = 348/384 (90.62%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MV D   QVA FSVALPSSLVTLPH+SS+RLSYHLP GLK+KVKKIK++SA DAGHS PP
Sbjct: 1   MVGDARVQVAAFSVALPSSLVTLPHKSSSRLSYHLPFGLKSKVKKIKATSA-DAGHSQPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSER NPLA+FLDVPRTVWR+TLRPLSNFGFG+RSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERTNPLALFLDVPRTVWRRTLRPLSNFGFGRRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIR+NPSLRCIET+VE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRVNPSLRCIETIVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           II+P NSLVEVNQSGLLMET+IDITPR+PIPVPS GPLDSECIKEGLILCDK KMKGHQG
Sbjct: 181 IIIPLNSLVEVNQSGLLMETMIDITPRDPIPVPSVGPLDSECIKEGLILCDKHKMKGHQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLD LVGIFTRLGREAEEIGL+NTF+LAQRVALV+EEA+PLLLKIQAMAEDVQPLLAE+
Sbjct: 241 VSLDALVGIFTRLGREAEEIGLTNTFSLAQRVALVVEEARPLLLKIQAMAEDVQPLLAEV 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLT SLSHATEDLRSVHASIMTPENT LLQKSIYTLI TLKNIESLSSEL
Sbjct: 301 RDSGLLKEVENLTSSLSHATEDLRSVHASIMTPENTELLQKSIYTLIHTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLK+LIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKLLIKSLSRLL 383

BLAST of Carg12324 vs. ExPASy TrEMBL
Match: A0A6J1C2W9 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007966 PE=4 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 7.2e-188
Identity = 348/384 (90.62%), Postives = 367/384 (95.57%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MV DTH QVA FS+ALP SLVTLP R+SNRL YHLPLGLK KVKKIK++S T+AGHS PP
Sbjct: 1   MVGDTHVQVAIFSMALPPSLVTLPRRTSNRLPYHLPLGLKTKVKKIKATS-TEAGHSQPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLA FLDVPRTVWRQTL PLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLAFFLDVPRTVWRQTLHPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVT+GNVIR+NPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTIGNVIRVNPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           II+P NSLVEVNQSGLLMET+IDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKG QG
Sbjct: 181 IIIPLNSLVEVNQSGLLMETMIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGQQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLD LVGIFTRLGREAEEIGLSNTF+LAQRVALVIEEAKPLLLKIQAMAEDV PLLAE+
Sbjct: 241 VSLDALVGIFTRLGREAEEIGLSNTFSLAQRVALVIEEAKPLLLKIQAMAEDVHPLLAEV 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGLLKEVENLT+SLS ATEDLRSVHAS+MTPENT LLQKSI+TLILTLKNIESLSS++
Sbjct: 301 RDSGLLKEVENLTRSLSLATEDLRSVHASVMTPENTELLQKSIHTLILTLKNIESLSSDV 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           +GFTGDEATKRNLK+LIKSLSRLL
Sbjct: 361 IGFTGDEATKRNLKLLIKSLSRLL 383

BLAST of Carg12324 vs. ExPASy TrEMBL
Match: A0A6J1HZ36 (protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468218 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.8e-187
Identity = 347/384 (90.36%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MVVDTHAQVAKFSVALPSSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPP 60
           MV DT  QVA FSVALPSSLVTLPHRSSNRLSYHLP GLK+KVKKIK++SA  AGHS PP
Sbjct: 1   MVGDTCVQVATFSVALPSSLVTLPHRSSNRLSYHLPFGLKSKVKKIKATSA-GAGHSQPP 60

Query: 61  SSSERRNPLAIFLDVPRTVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWL 120
           SSSERRNPLA+FLDVPRT+WR+TL PLSNFGFG+RSIWEGGVGLFLVSGAILLTLSLAWL
Sbjct: 61  SSSERRNPLALFLDVPRTLWRRTLHPLSNFGFGRRSIWEGGVGLFLVSGAILLTLSLAWL 120

Query: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDK 180
           RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIR+NPSLRCIETVVE+EDDK
Sbjct: 121 RGFQLRSKFRKYLAVFEFAQACGISTGTPVRIRGVTVGNVIRVNPSLRCIETVVEVEDDK 180

Query: 181 IIVPRNSLVEVNQSGLLMETVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQG 240
           II+P NSLVEVNQSGLLMET+IDITPR+PIPVPS GPLDS+CIKEGLILCDK KMKG+QG
Sbjct: 181 IIIPLNSLVEVNQSGLLMETMIDITPRDPIPVPSVGPLDSKCIKEGLILCDKHKMKGYQG 240

Query: 241 VSLDELVGIFTRLGREAEEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAEL 300
           VSLD LVGIFTRLGREAEEIGL+NTF+LAQRVALV+EEA+PLLLKIQAMAEDVQPLLAE+
Sbjct: 241 VSLDALVGIFTRLGREAEEIGLTNTFSLAQRVALVVEEARPLLLKIQAMAEDVQPLLAEV 300

Query: 301 RDSGLLKEVENLTKSLSHATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSEL 360
           RDSGL+KEVENLT SLSHATEDLRSVHASIMTPENT LLQKSIYTLI TLKNIESLSSEL
Sbjct: 301 RDSGLIKEVENLTSSLSHATEDLRSVHASIMTPENTELLQKSIYTLIHTLKNIESLSSEL 360

Query: 361 LGFTGDEATKRNLKVLIKSLSRLL 385
           LGFTGDEATKRNLK+LIKSLSRLL
Sbjct: 361 LGFTGDEATKRNLKLLIKSLSRLL 383

BLAST of Carg12324 vs. TAIR 10
Match: AT3G20320.1 (trigalactosyldiacylglycerol2 )

HSP 1 Score: 498.8 bits (1283), Expect = 3.9e-141
Identity = 257/367 (70.03%), Postives = 308/367 (83.92%), Query Frame = 0

Query: 18  SSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPPSSSERRNPLAIFLDVPR 77
           SS++  P  S N + Y LP   + +   ++++S +DA H   PSS   +NPL + LDVPR
Sbjct: 17  SSMIACPRVSPNGVPY-LPPKPRTRHLVVRAASNSDAAHG-QPSSDGGKNPLTVVLDVPR 76

Query: 78  TVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWLRGFQLRSKFRKYLAVFE 137
            +WRQTL+PLS+FGFG+RSIWEGGVGLF+VSGA LL LS AWLRGFQ+RSKFRKY  VFE
Sbjct: 77  NIWRQTLKPLSDFGFGKRSIWEGGVGLFIVSGATLLALSWAWLRGFQMRSKFRKYQTVFE 136

Query: 138 FAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVPRNSLVEVNQSGLL 197
            + A GI TGTPVRIRGVTVG +IR+NPSL+ IE V E+EDDKII+PRNSLVEVNQSGLL
Sbjct: 137 LSHASGICTGTPVRIRGVTVGTIIRVNPSLKNIEAVAEIEDDKIIIPRNSLVEVNQSGLL 196

Query: 198 METVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQGVSLDELVGIFTRLGREA 257
           MET+IDI PRNPIP PS GPL  EC KEGLI+CD+Q +KG QGVSLDELVGIFTR+GRE 
Sbjct: 197 METMIDIMPRNPIPEPSVGPLHPECGKEGLIVCDRQTIKGVQGVSLDELVGIFTRIGREV 256

Query: 258 EEIGLSNTFALAQRVALVIEEAKPLLLKIQAMAEDVQPLLAELRDSGLLKEVENLTKSLS 317
           E IG++NT++LA+R A VIEEA+PLL KIQAMAED QPLL+E RDSGLLKEVE LT+SL+
Sbjct: 257 EAIGVANTYSLAERAASVIEEARPLLKKIQAMAEDAQPLLSEFRDSGLLKEVECLTRSLT 316

Query: 318 HATEDLRSVHASIMTPENTALLQKSIYTLILTLKNIESLSSELLGFTGDEATKRNLKVLI 377
            A++DLR V++SIMTPENT L+QKSIYTL+ TLKN+ES+SS++LGFTGDEAT++NLK+LI
Sbjct: 317 QASDDLRKVNSSIMTPENTELIQKSIYTLVYTLKNVESISSDILGFTGDEATRKNLKLLI 376

Query: 378 KSLSRLL 385
           KSLSRLL
Sbjct: 377 KSLSRLL 381

BLAST of Carg12324 vs. TAIR 10
Match: AT3G20320.2 (trigalactosyldiacylglycerol2 )

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-98
Identity = 181/268 (67.54%), Postives = 216/268 (80.60%), Query Frame = 0

Query: 18  SSLVTLPHRSSNRLSYHLPLGLKAKVKKIKSSSATDAGHSVPPSSSERRNPLAIFLDVPR 77
           SS++  P  S N + Y LP   + +   ++++S +DA H   PSS   +NPL + LDVPR
Sbjct: 17  SSMIACPRVSPNGVPY-LPPKPRTRHLVVRAASNSDAAHG-QPSSDGGKNPLTVVLDVPR 76

Query: 78  TVWRQTLRPLSNFGFGQRSIWEGGVGLFLVSGAILLTLSLAWLRGFQLRSKFRKYLAVFE 137
            +WRQTL+PLS+FGFG+RSIWEGGVGLF+VSGA LL LS AWLRGFQ+RSKFRKY  VFE
Sbjct: 77  NIWRQTLKPLSDFGFGKRSIWEGGVGLFIVSGATLLALSWAWLRGFQMRSKFRKYQTVFE 136

Query: 138 FAQACGISTGTPVRIRGVTVGNVIRINPSLRCIETVVELEDDKIIVPRNSLVEVNQSGLL 197
            + A GI TGTPVRIRGVTVG +IR+NPSL+ IE V E+EDDKII+PRNSLVEVNQSGLL
Sbjct: 137 LSHASGICTGTPVRIRGVTVGTIIRVNPSLKNIEAVAEIEDDKIIIPRNSLVEVNQSGLL 196

Query: 198 METVIDITPRNPIPVPSAGPLDSECIKEGLILCDKQKMKGHQGVSLDELVGIFTRLGREA 257
           MET+IDI PRNPIP PS GPL  EC KEGLI+CD+Q +KG QGVSLDELVGIFTR+GRE 
Sbjct: 197 METMIDIMPRNPIPEPSVGPLHPECGKEGLIVCDRQTIKGVQGVSLDELVGIFTRIGREV 256

Query: 258 EEIGLSNTFALAQRVALVIEEAKPLLLK 286
           E IG++NT++LA+R A VIEEA+PLL K
Sbjct: 257 EAIGVANTYSLAERAASVIEEARPLLKK 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948293.11.4e-206100.00protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita moschata] >... [more]
XP_023523118.11.6e-20298.44protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita pepo subsp.... [more]
XP_022998942.11.7e-19996.88protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like [Cucurbita maxima] >XP... [more]
XP_023554510.19.4e-19091.67protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita ... [more]
XP_022952170.13.9e-18890.63protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 [Cucurbita ... [more]
Match NameE-valueIdentityDescription
Q9LTR25.4e-14070.03Protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q1XDB51.6e-1935.80Uncharacterized protein ycf22 OS=Pyropia yezoensis OX=2788 GN=ycf22 PE=4 SV=1[more]
P513727.6e-1735.04Uncharacterized protein ycf22 OS=Porphyra purpurea OX=2787 GN=ycf22 PE=4 SV=1[more]
P463158.7e-1329.25Uncharacterized protein ycf22 OS=Antithamnion sp. OX=2767 GN=ycf22 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G9G97.0e-207100.00protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like OS=Cucurbita moschata ... [more]
A0A6J1K9F48.3e-20096.88protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like OS=Cucurbita maxima OX... [more]
A0A6J1GJI31.9e-18890.63protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 OS=Cucurbit... [more]
A0A6J1C2W97.2e-18890.63protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic isoform X1 OS=Momordica cha... [more]
A0A6J1HZ362.8e-18790.36protein TRIGALACTOSYLDIACYLGLYCEROL 2, chloroplastic-like isoform X1 OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT3G20320.13.9e-14170.03trigalactosyldiacylglycerol2 [more]
AT3G20320.22.4e-9867.54trigalactosyldiacylglycerol2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003399Mce/MlaDPFAMPF02470MlaDcoord: 131..206
e-value: 4.8E-13
score: 49.2
IPR039342Protein TRIGALACTOSYLDIACYLGLYCEROL 2-likePANTHERPTHR34675FAMILY NOT NAMEDcoord: 5..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12324-RACarg12324-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport
cellular_component GO:0009706 chloroplast inner membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005319 lipid transporter activity
molecular_function GO:0005543 phospholipid binding