Carg12291 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12291
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description4-coumarate-CoA ligase
LocationCarg_Chr01: 2731620 .. 2734945 (+)
RNA-Seq ExpressionCarg12291
SyntenyCarg12291
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTCACGATTTCAGAATCTAAAAAATTTAAATCTCCTTCGCTTTCCGAGTCAAGGTTGAAGACGAAGACCCAAATTCTACGCACTTCCAGCCATTAATGGTGGCCATTTTTTCTTCCTACCTAATGCAGCAAACAACCATACACTGAGGTGGTCTCCTCACTCTTAATAGAAGTCTACTTACGTTTTTTCCGTTGCGAAGCAATTGAATCTGTTTTATCGTATCTTCTAAATTGCTTGTCTTCTTTTGTACTCGGACTCTTACGTTCTCTATTTAAATATTGATAGTAAATTTCCCCTCCAATCCGCTTCGGGTTGTGTGCAACTTCTTTATCTGAAACCTCTTTGCAATTGCGTCTGTGATTTTGATTCCTTCAATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGTACCCTGTAGTAGGCTGTTTGTGTTCTATAATTATGTTTTACTATAATCTATTGGATGCTTTTTCATTCTTTCACTTTCTTATATCAAGAACATCAAATGATATTTTCACTTAGAACCAACAGAGTTTGAAATGCAGAGGAGTTGGGACAATTAGAACTTAGAACAGTAGTGAAACACTTTTCTTTTAAATGCCTCCAAAAAGTACTTTTATGTATGTGTTAGAGATGACCTGTGACTTTTGAAGATAGAAAAACACTATTTAAGTAATCTTAGCAATTTCAAAAGAAGTTTTAACTAGGTTACCGAACACTGGTAAAATCAGCTTTCGTCGTTACAAAAGACATACCGACCCTAAGTGTTTATAGAATTCTCAAAATCTATGGTCATGCAATAAAAACCATGAAATTTTTAAAAGTTACTTCATTTGGTTAAAAATGCTTTTCCTATTCTAAAAGTCATATACATTCATCCTGACTATCGGCAACTCTAAATTCTGAATGGAACTTCTAAATGTTTTCACTTCATTTGGCTTTGATTTATAACCTTGTAGAGGTGGATTATAAGATTATAATGCAATAACTTGAATTCATTGCTATTCTCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTATATAGATATGGTTATCTTTCTCTTGATTTATGGTTTTCATACAATACTTTGGAGAGAATGTTTTTGTTTCGTAATCGAATACGATCTGAGTTAATGTGATCTTTAGTGTGTTGGGAGTTACTATTAGTACCTAACATTCAAGTCGGTTTTCGAATTCAATTTTGAAATGATGTGCAGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTACATATCTGTTTATCTAGTAATATTTAGCTCTAATCTCAGCAGTTTTAGCTTTCAGCTTTTGGAACTATGTCGAAACATTAGCATCCAGTAACTTCTTACCAATGATATCGTGCTATATCGGTCGTCCTTGTACGAGCAAGTGCTGTTTTGAGTTCAAATCAAATTCTTAAGCTGTCCTCCTCTCTCAAATTTTGTTACTGAATCTCCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCGTAAGCGTTCATGAAACTCCTTACTCGTGTTTGAACTTTCGACATTCACTCCTTTGTAACTGATTCGATGAAAACCGAATCAACAGCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTAAGGCACTTAAGAAGAAAGTTCGGTTAATTTCAGCAGCACGGATAGATTGCTCGATATGCATCAGGAATGAAACTTGTGTGTTTCTCCTGCAACAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAAGTTCTTTGGTTCCTCTTTTCATTACGACACGAGCAGAATAAGACCTTTTTGTTCTCTTATTTTCACACTTTTATGTAATTTCTTTTTAATATAACTAACTATGTAGTTCAAGTTTCTATAATAATTTACTTTT

mRNA sequence

TTTTTTTTTCACGATTTCAGAATCTAAAAAATTTAAATCTCCTTCGCTTTCCGAGTCAAGGTTGAAGACGAAGACCCAAATTCTACGCACTTCCAGCCATTAATGGTGGCCATTTTTTCTTCCTACCTAATGCAGCAAACAACCATACACTGAGGTGGTCTCCTCACTCTTAATAGAAGTCTACTTACGTTTTTTCCGTTGCGAAGCAATTGAATCTGTTTTATCGTATCTTCTAAATTGCTTGTCTTCTTTTGTACTCGGACTCTTACGTTCTCTATTTAAATATTGATAGTAAATTTCCCCTCCAATCCGCTTCGGGTTGTGTGCAACTTCTTTATCTGAAACCTCTTTGCAATTGCGTCTGTGATTTTGATTCCTTCAATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAAGTTCTTTGGTTCCTCTTTTCATTACGACACGAGCAGAATAAGACCTTTTTGTTCTCTTATTTTCACACTTTTATGTAATTTCTTTTTAATATAACTAACTATGTAGTTCAAGTTTCTATAATAATTTACTTTT

Coding sequence (CDS)

ATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAA

Protein sequence

MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVRAKI
Homology
BLAST of Carg12291 vs. NCBI nr
Match: KAG6607161.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036848.1 4-coumarate--CoA ligase-like 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1062.0 bits (2745), Expect = 1.7e-306
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_022949355.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.0e-306
Identity = 542/543 (99.82%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AK+
Sbjct: 541 AKL 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_023525684.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.0e-304
Identity = 539/543 (99.26%), Postives = 540/543 (99.45%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASF+FRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFIFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVK LNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKS SE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKVLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSSSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRN ITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNLITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_022998874.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.6e-302
Identity = 535/543 (98.53%), Postives = 538/543 (99.08%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFP DPNLSVASFLFRNSSSYPNRLAIVDAESSDS SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVY VSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLD+QIP EIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENR VGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_038895538.1 (4-coumarate--CoA ligase-like 7 [Benincasa hispida])

HSP 1 Score: 976.1 bits (2522), Expect = 1.2e-280
Identity = 492/543 (90.61%), Postives = 518/543 (95.40%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+ PNLS+ SFLFRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKHPNLSMISFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KAS I++SNGLLQLGIEKNDVV+IFAPNSV++SICF+GI+AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASVIRVSNGLLQLGIEKNDVVLIFAPNSVQYSICFLGIIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVKDLNIPTVLLDQQIPS I SSK+LCFNDL+NMAGDKSGS+
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQQIPSAIHSSKLLCFNDLMNMAGDKSGSD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIV VKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMIT+DQ   G E G YLNFLP
Sbjct: 181 FPIVEVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITMDQTFRGKEYGVYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLK +GSGAAPLGKELM+EC NNIPSAVVLQGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKRVGSGAAPLGKELMEECGNNIPSAVVLQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDE GQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPD EAGE
Sbjct: 421 TGDLGYFDEYGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDAEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 8.8e-221
Identity = 380/546 (69.60%), Postives = 467/546 (85.53%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDG+FRSLRPP+V P+D NLS+ SF+FRNSSSYP + A++D+++++++S+SQ 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K+  IK+S+G L LG++KNDVV+IFAPNS+   +CF+GIVA GAI TT NP+Y VSEL+K
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLL--DQQIPSEIQS-SKILCFNDLINMAGDKS 180
           QV+D+ PKL+++V EL++KVK  N+PT+L+  + +  S ++S +K+L F+DL+ ++G   
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPV- 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 240
            S+FP+V  KQSDTAALLYSSGTTG SKGV+L+H+NFI SSLM+T++Q   G+ +  +L 
Sbjct: 181 -SDFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLC 240

Query: 241 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGL  IT AQLQRGNT++SM +F+LEK L  VEKYKVT LWVVPPV+LAL K 
Sbjct: 241 FLPMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKN 300

Query: 301 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 360
           S+VKKY+LSSLKHIGSGAAPLGK+LM+ECA  +P  +V QGYGMTETCG+VS+E+   GK
Sbjct: 301 SIVKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGK 360

Query: 361 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 420
           R+TGSAG L+SGVEAQIVSVDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK TIDK G
Sbjct: 361 RHTGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKG 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 480
           WVHTGDLGYFDE+G LYVVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP+PD +
Sbjct: 421 WVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAD 480

Query: 481 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 540
           AGEVP+AYVVRS NSS+TE+DV KF+A QV+ +KRLR+VTFINSVPKS SGKILRRELI+
Sbjct: 481 AGEVPVAYVVRSPNSSLTEDDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQ 540

Query: 541 KVRAKI 544
           KVR+ I
Sbjct: 541 KVRSNI 544

BLAST of Carg12291 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 2.9e-211
Identity = 362/546 (66.30%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGI+RSLRP +V P+DPN S+ SFLFRNSSSYP++LAI D+++ DS+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  ++++G  +LGI KNDVV+IFAPNS +F +CF+ + AIG + TT NP+Y V+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEI---QSSKILCFNDLINMAGDKS 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL  +   EI    +SKIL F++++ ++  + 
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELS--EP 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 240
            SE+P V +KQSDTAALLYSSGTTG SKGV LTH NFI +SLM+T+DQ   G+ +G +L 
Sbjct: 181 VSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLC 240

Query: 241 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGL  IT +QLQRGN +VSM +F LE  L  +EK++VT LWVVPPV LAL+KQ
Sbjct: 241 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 300

Query: 301 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 360
           S+VKK++LSSLK+IGSGAAPLGK+LM+EC  NIP+ +++QGYGMTETCG+VS+E+  +GK
Sbjct: 301 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGK 360

Query: 361 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 420
           RN+GSAG LA GVEAQIVSV+T K  PPNQ GEI VRGPNMM GYLNNPQATK+TIDK  
Sbjct: 361 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 480
           WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP+PD+E
Sbjct: 421 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 480

Query: 481 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 540
           AGEVPIA+VVRS NSS+TE+D+ KF+A QV+PYKRLRRV+FI+ VPKS +GKILRREL++
Sbjct: 481 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 540

Query: 541 KVRAKI 544
           +VR+K+
Sbjct: 541 QVRSKM 544

BLAST of Carg12291 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 7.8e-185
Identity = 327/544 (60.11%), Postives = 419/544 (77.02%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEK       I+ S RPP+ FP DP LS+ SFLFR+S+SYPNR A++DA+S  ++++ +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K    K+++ L+QL I+KNDVV+IFAPNS+ F +CF  I A+GAI TTCNP Y  +EL+ 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           Q +D  P L+I+V ELW+K + LN+P ++L     S++ S     F   +    D+  SE
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRF-SE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
            PI  V+QSD AALLYSSGTTG SKGV+L+H+NFIT+SLM+T DQ   GD     + FLP
Sbjct: 181 LPISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-V 300
           MFH+FGL  I  +QL+RGN +VSM KF LE AL AVE Y+VT L+VVPPV++ALAK+S V
Sbjct: 241 MFHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVV 300

Query: 301 VKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRN 360
           V++Y+LSS+K I SGAAPLGK +M++CA N+P A ++QGYGMTETCG++S+E+   G R 
Sbjct: 301 VRRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRF 360

Query: 361 TGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWV 420
           +GS G LA G+E+QI+      PLPPNQ GEI +RGPNMM GYLNN +ATK TID+ GWV
Sbjct: 361 SGSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWV 420

Query: 421 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAG 480
            TGD+GYFDE GQL+VVDR+KELIK  GFQVAPAELEALL+SHPEILDAVVIP+PD++AG
Sbjct: 421 KTGDIGYFDEEGQLFVVDRLKELIKCYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAG 480

Query: 481 EVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKV 540
           EVPIA VVRS NSS++EEDV +F+  QV+P+K+LRRVTF++SV KS +GKILRRELI+KV
Sbjct: 481 EVPIANVVRSPNSSLSEEDVQRFIEKQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKV 540

Query: 541 RAKI 544
           R+KI
Sbjct: 541 RSKI 543

BLAST of Carg12291 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 565.8 bits (1457), Expect = 5.1e-160
Identity = 297/545 (54.50%), Postives = 394/545 (72.29%), Query Frame = 0

Query: 4   SGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSD-SVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R + + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  SAIKISNGL-LQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 123
           + +  +  L  + G+   D V++ APN V + +CF  + A+GA+ TT NP Y   E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQS----SKILCFNDLINMAGDKS 183
           V DA+ KL+I++  L  K+  L +P +LLD    +   S    + +  + +L+  AG K 
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLV--AGVKE 188

Query: 184 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 243
            +++    +KQSDTAALLYSSGTTG SKGVILTHRNFI ++ M+T DQ    +    +L 
Sbjct: 189 -ADYRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLC 248

Query: 244 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 303
           FLPMFH+FGL  IT AQL RGN I++M +F++   + AV++++VT L+ VPPV++ALAK 
Sbjct: 249 FLPMFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKH 308

Query: 304 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 363
               KY+LSSLK IGSGAAPLGK++M+  A   P + ++QGYGMTETCG++SLE    G+
Sbjct: 309 GKAGKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQ 368

Query: 364 -RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKY 423
            R  GS GTL SGVEA+IV + TLK LPPNQ GEI VRGPN+M GY NN QAT+ TI K 
Sbjct: 369 AREFGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQ 428

Query: 424 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDD 483
           GW+HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP+PD 
Sbjct: 429 GWLHTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDA 488

Query: 484 EAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELI 542
           +AGEVPIAYVVRS +SS+TE DV KF+  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI
Sbjct: 489 KAGEVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLI 548

BLAST of Carg12291 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 8.5e-123
Identity = 231/545 (42.39%), Postives = 360/545 (66.06%), Query Frame = 0

Query: 2   EKSGYGR-DGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 61
           E+SGY + + IF S R P+  P++ ++ V +F+  +S ++  ++A +DA +   +++ QL
Sbjct: 11  ERSGYCKSNSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQL 70

Query: 62  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 121
             +   ++  L  +GI K DV+++ +PNS+ F +  + ++++GAI+TT NP+    E+ K
Sbjct: 71  WRAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAK 130

Query: 122 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 181
           Q+ D+KP L  ++ +L  K+   N+P V++D ++ S ++ + +   + L  M   +    
Sbjct: 131 QITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKT-LNIVSSLGEMMRKEPSPN 190

Query: 182 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 241
                V Q DTA LLYSSGTTGASKGV+ +H+N I + +   L +    D   T++  +P
Sbjct: 191 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLI-AMVQTILSRFGTEDGEHTFICTVP 250

Query: 242 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-- 301
           MFH++GL       L  G+TIV + KF + + L A+EKY+ T L +VPP+++AL K +  
Sbjct: 251 MFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANH 310

Query: 302 VVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKR 361
           +  KY+LSSL+ + SG APL KE+++    N P+  +LQGYG+TE+ G+ +  +     R
Sbjct: 311 IRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESR 370

Query: 362 NTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGW 421
             G+AG L+  +EA+IV+ +T + L  N+ GE+ +RGP +M GY +N +AT  TID  GW
Sbjct: 371 RYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGW 430

Query: 422 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEA 481
           + TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL+SHPEI DA VIPYPD EA
Sbjct: 431 LRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEA 490

Query: 482 GEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEK 541
           G+ P+AYVVR   S+++E  V+ F+A  V+PYKR+R+V F+ S+PK+ SGKILR++LI+ 
Sbjct: 491 GQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKL 550

Query: 542 VRAKI 544
             +K+
Sbjct: 551 ATSKL 551

BLAST of Carg12291 vs. ExPASy TrEMBL
Match: A0A6J1GCI6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111452734 PE=4 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.4e-306
Identity = 542/543 (99.82%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AK+
Sbjct: 541 AKL 543

BLAST of Carg12291 vs. ExPASy TrEMBL
Match: A0A6J1KDQ6 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111493400 PE=4 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.3e-302
Identity = 535/543 (98.53%), Postives = 538/543 (99.08%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFP DPNLSVASFLFRNSSSYPNRLAIVDAESSDS SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVY VSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLD+QIP EIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENR VGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. ExPASy TrEMBL
Match: A0A5D3DCX7 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00860 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.7e-279
Identity = 485/543 (89.32%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSG GRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVVMIFAPNSV+ SICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVKDLNIPTVLLDQ+IPS I SSKILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFI SSLMIT+DQ  +G+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI++VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. ExPASy TrEMBL
Match: A0A1S3CIB0 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.7e-279
Identity = 485/543 (89.32%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSG GRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVVMIFAPNSV+ SICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVKDLNIPTVLLDQ+IPS I SSKILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFI SSLMIT+DQ  +G+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI++VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. ExPASy TrEMBL
Match: A0A0A0LXU2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.7e-278
Identity = 483/543 (88.95%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 736.1 bits (1899), Expect = 2.0e-212
Identity = 362/546 (66.30%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGI+RSLRP +V P+DPN S+ SFLFRNSSSYP++LAI D+++ DS+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  ++++G  +LGI KNDVV+IFAPNS +F +CF+ + AIG + TT NP+Y V+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEI---QSSKILCFNDLINMAGDKS 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL  +   EI    +SKIL F++++ ++  + 
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELS--EP 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 240
            SE+P V +KQSDTAALLYSSGTTG SKGV LTH NFI +SLM+T+DQ   G+ +G +L 
Sbjct: 181 VSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLC 240

Query: 241 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGL  IT +QLQRGN +VSM +F LE  L  +EK++VT LWVVPPV LAL+KQ
Sbjct: 241 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 300

Query: 301 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 360
           S+VKK++LSSLK+IGSGAAPLGK+LM+EC  NIP+ +++QGYGMTETCG+VS+E+  +GK
Sbjct: 301 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGK 360

Query: 361 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 420
           RN+GSAG LA GVEAQIVSV+T K  PPNQ GEI VRGPNMM GYLNNPQATK+TIDK  
Sbjct: 361 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 480
           WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP+PD+E
Sbjct: 421 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 480

Query: 481 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 540
           AGEVPIA+VVRS NSS+TE+D+ KF+A QV+PYKRLRRV+FI+ VPKS +GKILRREL++
Sbjct: 481 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 540

Query: 541 KVRAKI 544
           +VR+K+
Sbjct: 541 QVRSKM 544

BLAST of Carg12291 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 418.7 bits (1075), Expect = 7.2e-117
Identity = 218/540 (40.37%), Postives = 350/540 (64.81%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLK 62
           +SG+   +  F S R PI  P +P+L V +F+  +S ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 122
            +   +++ L ++GI K  VV++ +PNS+ F +  + ++++GAI+TT NP+   +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLMISVVELWDKVKDL--NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGS 182
           ++D+ P L  +  +L  K+      +P VL+D++    +   +      L+ M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFL 242
                 V Q DTA LLYSSGTTG SKGVI +HRN I + +   +++  + D    ++  +
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLI-AMVQTIVNRFGSDDGEQRFICTV 246

Query: 243 PMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK--Q 302
           PMFH++GL       L  G+TI+ + KF + + + A+ KY+ T L +VPP+++A+     
Sbjct: 247 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 306

Query: 303 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 362
            +  KY+LSS+  +  G APL KE+ +  A   P+  +LQGYG+TE+ G+ +  +     
Sbjct: 307 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 366

Query: 363 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 422
           R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GY +N +AT  T+D  G
Sbjct: 367 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 426

Query: 423 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 482
           W+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP+PD E
Sbjct: 427 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKE 486

Query: 483 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 538
            G+ P+AYVVR T SS++E+ +++FVA QV+PYKR+R+V F++S+PK+ SGKILR++LI+
Sbjct: 487 VGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of Carg12291 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 402.5 bits (1033), Expect = 5.3e-112
Identity = 231/549 (42.08%), Postives = 339/549 (61.75%), Query Frame = 0

Query: 3   KSGYGRD-GIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLK 62
           KSG+     IF S R P+  P +  L V SF+   S  +  +   VDA +   +S+ +L 
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSFI--ASQPHRGKTVFVDAVTGRRLSFPELW 83

Query: 63  ASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 122
               +++  L  LG+ K +VV+I +PNS+ F I  + ++++GAI+TT NP+    E++KQ
Sbjct: 84  LGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQ 143

Query: 123 VRDAKPKLMISVVELWDKV---KDLNIPTVLLDQ-QIPSEIQSSKILCFNDLINMAGDKS 182
           + D++P L  +  +L  K+    + N+P VL+D   +PS+    ++     L  M   + 
Sbjct: 144 IGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEP 203

Query: 183 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 242
                   V Q DTAALLYSSGTTG SKGV+L+HRN I    ++   +   G E  T   
Sbjct: 204 SESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA---LVQAYRARFGLEQRTICT 263

Query: 243 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK- 302
            +PM H+FG        +  G TIV +PKF++ K L AVE ++ + L +VPP+V+A+   
Sbjct: 264 -IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVNG 323

Query: 303 -QSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGV-VSLENRT 362
              +  KY+LSSL  + +G APL +E+ ++   N P   +LQGYG+TE+  +  S+ N+ 
Sbjct: 324 ANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNKE 383

Query: 363 VGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTID 422
             KR  G++G LA  VE +IV  DT + L  NQ GE+ +R P +M GY  N +AT  TID
Sbjct: 384 ETKR-YGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 443

Query: 423 KYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYP 482
             GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP P
Sbjct: 444 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 503

Query: 483 DDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRE 542
           D +AG+ P+AY+VR   S+++E +++ FVA QVSPYK++R+VTF+ S+PK+ SGKILRRE
Sbjct: 504 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 563

Query: 543 LIEKVRAKI 544
           L +   +K+
Sbjct: 564 LTKLTTSKL 565

BLAST of Carg12291 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 399.4 bits (1025), Expect = 4.5e-111
Identity = 228/541 (42.14%), Postives = 333/541 (61.55%), Query Frame = 0

Query: 11  IFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKISNG 70
           IFRS  P I  P   +L + ++ F   SS  ++  ++   +  S +Y +      ++++G
Sbjct: 34  IFRSKLPDIDIPN--HLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASG 93

Query: 71  LLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPKLM 130
           L +LGI K DV+MI   NS EF   F+G   IGA+ TT NP Y   EL KQ++ +  KL+
Sbjct: 94  LYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLI 153

Query: 131 ISVVELWDKVKDL--NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVGVKQ 190
           I+  +  DK+K+L  N+  +  D+  P        L F+ LI    D++      V +  
Sbjct: 154 ITHSQYVDKLKNLGENLTLITTDEPTP-----ENCLPFSTLI--TDDETNPFQETVDIGG 213

Query: 191 SDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN-------FLPM 250
            D AAL +SSGTTG  KGV+LTH++ ITS     + Q+ +GD    YL         LP+
Sbjct: 214 DDAAALPFSSGTTGLPKGVVLTHKSLITS-----VAQQVDGDNPNLYLKSNDVILCVLPL 273

Query: 251 FHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVVK 310
           FH++ L  +    L+ G T++ M KF +   L  +++++VT   +VPP+V+ALAK   V 
Sbjct: 274 FHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVN 333

Query: 311 KYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLE---NRTVGKR 370
            Y+LSS++ + SGAAPLGKEL D     +P A++ QGYGMTE   V+S+     +     
Sbjct: 334 SYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPT 393

Query: 371 NTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGW 430
            +GS GT+    E ++V ++T   L  NQ GEI +RG  +M  YLN+P+AT  TID+ GW
Sbjct: 394 KSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGW 453

Query: 431 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEA 490
           +HTGD+GY DE+ ++++VDR+KE+IK+KGFQV PAELE+LL++H  I DA V+P  D+ A
Sbjct: 454 LHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVA 513

Query: 491 GEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEK 540
           GEVP+A+VVRS  + +TEEDV ++VA QV  YKRL +V F+ S+PKS SGKILR++L  K
Sbjct: 514 GEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAK 560

BLAST of Carg12291 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 224/542 (41.33%), Postives = 338/542 (62.36%), Query Frame = 0

Query: 9   DGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKIS 68
           D IFRS  P I  P   +L +  ++F N S +  +  +++  + +  +Y+ +  ++ K++
Sbjct: 20  DVIFRSRLPDIYIPN--HLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 79

Query: 69  NGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPK 128
            GL  LG++++DVVMI  PNS E  + F+    IGAI T+ NP +  +E++KQ + +  K
Sbjct: 80  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 139

Query: 129 LMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVG--- 188
           L+++     DK+K+L    VL+     S+      L F++L       + SE P V    
Sbjct: 140 LIVTQSRYVDKIKNLQNDGVLI-VTTDSDAIPENCLRFSEL-------TQSEEPRVDSIP 199

Query: 189 --VKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN------ 248
             +   D  AL +SSGTTG  KGV+LTH+  +TS     + Q+ +G+    Y N      
Sbjct: 200 EKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTS-----VAQQVDGENPNLYFNRDDVIL 259

Query: 249 -FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 308
             LPMFH++ L  I    L+ G TI+ MPKF +   L  +++ KVT   VVPP+VLA+AK
Sbjct: 260 CVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAK 319

Query: 309 QSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLE---NR 368
               +KY+LSS++ + SGAAPLGKEL D  +   P+A + QGYGMTE   V+++     +
Sbjct: 320 SPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 379

Query: 369 TVGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTI 428
                 +G+ GT+    E +I+  DT   LP N+ GEI +RG  +M GYLN+P AT  TI
Sbjct: 380 EPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTI 439

Query: 429 DKYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPY 488
           DK GW+HTGD+G+ D++ +L++VDR+KELIKYKGFQVAPAELE+LL+ HPEI D  V+  
Sbjct: 440 DKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAM 499

Query: 489 PDDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRR 536
            +++AGEVP+A+VVRS +S+++E+++ +FV+ QV  YKR+ +V F +S+PK+ SGKILR+
Sbjct: 500 KEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRK 546

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6607161.11.7e-306100.004-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_022949355.13.0e-30699.824-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate-... [more]
XP_023525684.18.0e-30499.264-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-co... [more]
XP_022998874.12.6e-30298.534-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--C... [more]
XP_038895538.11.2e-28090.614-coumarate--CoA ligase-like 7 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
M4IRL68.8e-22169.60Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1[more]
Q9M0X92.9e-21166.304-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
M4IQS17.8e-18560.11Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1[more]
Q0DV325.1e-16054.504-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1... [more]
M4IQR78.5e-12342.39Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GCI61.4e-30699.824-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111452734 PE=... [more]
A0A6J1KDQ61.3e-30298.534-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111493400 PE=4 ... [more]
A0A5D3DCX79.7e-27989.324-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CIB09.7e-27989.324-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1[more]
A0A0A0LXU23.7e-27888.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G05160.12.0e-21266.30AMP-dependent synthetase and ligase family protein [more]
AT1G20510.17.2e-11740.37OPC-8:0 CoA ligase1 [more]
AT1G20480.15.3e-11242.08AMP-dependent synthetase and ligase family protein [more]
AT1G65060.14.5e-11142.144-coumarate:CoA ligase 3 [more]
AT3G21240.11.1e-10941.334-coumarate:CoA ligase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 441..542
e-value: 5.5E-31
score: 109.3
NoneNo IPR availablePANTHERPTHR24096:SF3574-COUMARATE:COA LIGASE-LIKE PROTEINcoord: 4..542
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 4..542
NoneNo IPR availableCDDcd059044CLcoord: 21..535
e-value: 0.0
score: 727.492
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 17..541
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 454..529
e-value: 4.3E-15
score: 56.5
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 10..440
e-value: 2.9E-129
score: 433.5
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..445
e-value: 2.2E-102
score: 342.8
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12291-RACarg12291-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016405 CoA-ligase activity