Carg12258 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12258
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionvacuolar protein sorting-associated protein 32 homolog 2-like
LocationCarg_Chr11: 2965923 .. 2969404 (+)
RNA-Seq ExpressionCarg12258
SyntenyCarg12258
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAATTGTTTAGGTTTTAGGTTTAGTAAGTCAGCTTAACCTTTTCTTTTGTGGGCCGCAACACTGTCAACGCCGTGAAGAAAAAGGCATCTCCCACCCAACAACAACGCGCCTAAGTAAACCGGACCAAAGTCGCCTCTCAACCAGATCTGCTCTTCTTCTTTAATTTTATTTCTCCACACCGATCGCTCTTCGATTTCCTATCATCACCTCTCAGGTACACACCTCTTCATCAATTTTTTCTTTTTTTTTTCGGGATCGAATTGACCTTGTGTTTCAATCTCGATTTTGGTCGAGTTTCGAGATCTGATTTCTGGTCAGACGAGCTATGAGTGATGATTTTGTTTCTGTTGATGCAGAAGTCCGGAGGTCGGTTCGATTCGCAGATAATCCACCATGTCTATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTAGTGCCCTGGCGACGCTTGATAAATTAAACGAGGTATCATGGCGGACTCGGTATCTTTCAATTGAATTCCTTCTCCCCACTGCTGTTTATTAGGGATTCTTGAGAGTTTTTGAAAATTGAAACCACTAATATGGCTAAGTCGAGCTACAAGATTTTCCCAACATCACTAATTGCTGTTCTTTGTTATAGCCACGACTCTTTTAGGCCGAAATGAATTGTTTTATGCTCTGTAAATTTTCAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCATCAACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGAGGTATGGCAGTTTATTATTGAACGAAACATTTTGGCGTTAAGTACTTGCAAACTGTTGTGATATTATGATGAACTTGTTGATGATTAACGTCAAGGGTGGCATAGTGTAATGAAACAACCAATGATTTTTGTTATCTTTTCTTCTTCTACTTTGTTCGGGGGATGGAGGGGAGGGGCCTCGGATGTTGATATCCAAATTCATTTTCGATTCCTTCTTGGAGTTGAATACCTCAGTCAAGAATTGTTTTTGATCCAATCCATAGGAGTCGGTAGAAAAGACAAGTACCTTATGATACACCAAATCGGGCTCGATTATTGATAGAGTGAATAGATTGTTTGACTTTTTCCCATTTGAAATGTTTGTAGTCTTTGGACGTCCCTATATGTAAAGGGTACTTCAATACTTACTAGTTTTGCAATTTCTAAACGTGCTTCCTATTAAGTTAGGTGTTCTTTAGTCAAGGAAATAACTTATTGATGTGTGGTTACTGTGAGATCACATGGGAACGAAGCATGAGTGTGGAAACCTCTCTCTAGTAGACGTGATTTTCTCTATAAGGGTGTGGAAACCTCTCTCTAGTAGATGCGTTTTAAAATTGTGAGGCTGACGGTAATACGTAACGGGTAAAAGCAGACAATATCTACTAGAGGTGGGCTTGAGTTGTTTACAAATGGTATCAGAGCCAAACACCGGGCGGTGTGCCAGCGAGGACGCTGGGCTCAAGGGGGGTGTATTGTGAGATCCCACATTGGTTGGAGAGGAGAACGAAGTATTTCCTATAAAGGTGTAGAAACCTCTCCCTAGCAGACACGTTTCCATGAGACTGACGGCGGTATGTAACAAGTCAAAGCGGACAATATTTGCTGTTACAGTTACAGTCAAGTTTTAACGAGTCATGTTGTTATTAATGTGGTAAATGGCTTGTTTTGGCCTTCTTTCATTCAGTGATTTATGCTCTTCTATTTCTTAATTTCAGCTCTGCTAGTTATGTTTGTTTCTTATCCAAGTTGGGGATGTGTTTAATTTTCAGCGTGGTTGCAAGATAATTAGTTGGTTATACCTCTTGATCTTGGGGTGTTTGCATTTCCATTTTTCGTATGCCCCAACTTCCATCACAAATTTCTGGGAACCTGTTGGCTTCTTTAGTTCCAAGTATTCATGTGTCTTGGAGAAGTTTATGATAGGAGCCGTTTGTTTCCTTCCTTTGATGTTGGATCTTTCCCACTTTCAGTTTGCAATTCAAACTGTTTTTGGTGGTAATATCGCTTCAATATTAACTTTTTTTAAAAAACTTTATAGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAGCAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATCCACGATCAGGTGATTTTTTTATGTTATATTGTTACTGTGAATCCCCACATTGGCTGGGGAGGGGAACGAAGCATGTCTTTTGAGGGGAAGGTCTAAAGGGAAAGCCCAAAGAAGATAATATTTGCTGGTGGTGGGCTTGGGTTGTTACAAATGGTATTAGAGCTAGACTCCGGGCGGTGTTGCCAGCGAGGACACTGGGCCCCAGAGGGTGGATTGTGAGATCCAACATTGGCTGGAGAGGAGATGAGTATGAGTCATGACTTTTAAAGGTGTGGAAACCTCTCCCTGGGCGGTGTGCCACCGAGGACACTAGACCCCCAAGAGGGTGGATTGTGAGATCCAACATTGGCTGGAGAGGGGATGAGTCATGACTTTTAAGGGTGTGGAAACTTCTCCCTAGCAGACGCGCTTTAGAATCTTTGAGGGGAAGTTCGAAAGGGAAAAGCCCAAAAAGGACAGAACTGCTAGCAGTGGACTTGAACGGGAAAAGTTCAAAAAGGACAGAACTGCTAGCAGTGGACTTGAACGGGAAAAGTTCAAAAAAGACAGAACTGCTAGCAGTGGACTTGAACGATTAGAGATACATAGTTTGATGTATTTATGTTTGCTTTTGGTGTTTGTGTATCTAACGTTGTGAGTTATAAATATTTGGGGTGCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGTAACGACACATTCTTTCAATGTAGTTTCATTATTTGTATTTAGGTTCTCTTTACCCATCCAAAGGTTGTGAAAATCTTCGGAATGATGTGCTGGCGGAGCATAACAACGCATGATGCCCGAGGGCCGAATGTTTCCATTACAATTTATGAAATTGGATGTTTAATAGTCTCCTTTTGCAGGAATATCGATGATGTCGATAAAACAATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCTTCAGCTGATTTTGATGAGGTTTTTACTCACTTTCCGTGTGTTCTACCATTCGTCCCTGCAATTTTGTTTAGTTTTTCTTCCACTAAACACTTGTTTGTACGCACAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCAGAGTTGGAAGAACAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCGCCAATTCCTGTCCCAGCGGGCAGACAAACTGCTCGACCTGCTCCAAAACGAACTGCTGAAGAAGATGAACTGGCAGCTCTGCAGGCTGAGATGGCTCTTTGA

mRNA sequence

AAAAATTGTTTAGGTTTTAGGTTTAGTAAGTCAGCTTAACCTTTTCTTTTGTGGGCCGCAACACTGTCAACGCCGTGAAGAAAAAGGCATCTCCCACCCAACAACAACGCGCCTAAGTAAACCGGACCAAAGTCGCCTCTCAACCAGATCTGCTCTTCTTCTTTAATTTTATTTCTCCACACCGATCGCTCTTCGATTTCCTATCATCACCTCTCAGAAGTCCGGAGGTCGGTTCGATTCGCAGATAATCCACCATGTCTATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTAGTGCCCTGGCGACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCATCAACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGAGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAGCAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATCCACGATCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATCGATGATGTCGATAAAACAATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCTTCAGCTGATTTTGATGAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCAGAGTTGGAAGAACAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCGCCAATTCCTGTCCCAGCGGGCAGACAAACTGCTCGACCTGCTCCAAAACGAACTGCTGAAGAAGATGAACTGGCAGCTCTGCAGGCTGAGATGGCTCTTTGA

Coding sequence (CDS)

ATGTCTATGTTCAATAAGCTTTTTGGTAAACCTAAGCAGGAGTCTAGTGCCCTGGCGACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCATCAACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGAGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAGCAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATCCACGATCAGATGATATTATTAGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATCGATGATGTCGATAAAACAATGGACGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTATCAAATCCAATTGGTGCTTCAGCTGATTTTGATGAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCAGAGTTGGAAGAACAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCGCCAATTCCTGTCCCAGCGGGCAGACAAACTGCTCGACCTGCTCCAAAACGAACTGCTGAAGAAGATGAACTGGCAGCTCTGCAGGCTGAGATGGCTCTTTGA

Protein sequence

MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL
Homology
[Close]
BLAST of Carg12258 vs. NCBI nr
Match: XP_022933834.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_023005480.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_023530625.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >KAG7021910.1 Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.4 bits (1012), Expect = 6.3e-106
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. NCBI nr
Match: XP_008450809.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008450810.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_016900986.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >TYK10128.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 385.2 bits (988), Expect = 3.8e-103
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. NCBI nr
Match: XP_004135686.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011659962.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031737801.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN66200.1 hypothetical protein Csa_006989 [Cucumis sativus])

HSP 1 Score: 385.2 bits (988), Expect = 3.8e-103
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELE AELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. NCBI nr
Match: XP_022961241.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961242.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961243.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961244.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022987856.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987857.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987858.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987859.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >KAG7023632.1 Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.9 bits (982), Expect = 1.9e-102
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQES+ALATLDKLNETLEMLEKKE VLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAP APIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. NCBI nr
Match: XP_038880238.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida])

HSP 1 Score: 381.7 bits (979), Expect = 4.2e-102
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPI VPAGRQTARP P KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 3.5e-91
Identity = 188/219 (85.84%), Postives = 203/219 (92.69%), Query Frame = 0 

Query: 3   MFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE++AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-IPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           AP  P +PVPAGRQ ARP P KRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 5.4e-84
Identity = 183/217 (84.33%), Postives = 196/217 (90.32%), Query Frame = 0 

Query: 5   NKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAIQC 64
           N+LFGKPKQE+S L TLDKLNETLEMLEKKE VLLKKA+ EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 65  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 124
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 125 DKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAP 184
           DKTMDEINEQTENMKQIQEALS P GA+ DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFGAN-DFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 185 AAPIPVPAGRQTAR-PAPKR-TAEEDELAALQAEMAL 220
             PI VP G + AR PA K+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

[Close]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 8.7e-26
Identity = 92/219 (42.01%), Postives = 126/219 (57.53%), Query Frame = 0 

Query: 1   MSMFNKLF---GKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKN 60
           MS+F K+F   GK  + +S    + +L ET EML KK++ L KK   E+  AK     KN
Sbjct: 1   MSLFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLL 180
             +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPIPVPAGRQTARPAPKRTAE-EDELAALQA 216
           +     P     VP+    +RPA K+  E ED++  L+A
Sbjct: 181 EIGDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

[Close]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 89/213 (41.78%), Postives = 122/213 (57.28%), Query Frame = 0 

Query: 1   MSMFNKLF---GKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKN 60
           MS+  KLF   GK  +  S    + KL +T EML KK++ L KK   E+  AK +   KN
Sbjct: 1   MSLIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLL 180
             +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPIP-VPAGRQTARPAPKRTAEEDE 210
           +        P+P VPA    A+P  K+  E+D+
Sbjct: 181 E-VQGPETVPLPNVPAASLPAKPVKKKQEEDDD 211

[Close]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match: Q6GL11 (Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-25
Identity = 89/213 (41.78%), Postives = 122/213 (57.28%), Query Frame = 0 

Query: 1   MSMFNKLF---GKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKN 60
           MS+  KLF   GK  +  S    + KL +T EML KK++ L KK   E+  AK +   KN
Sbjct: 1   MSLIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLTKKQEFLEKKIEQELVTAKKH-GTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLL 180
             +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPIP-VPAGRQTARPAPKRTAEEDE 210
           +        P+P VPA    A+P  K+  E+D+
Sbjct: 181 E-VQGPETVPLPNVPAAVLPAKPVKKKQEEDDD 211

[Close]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match: A0A6J1KXI2 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111498451 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.0e-106
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match: A0A6J1F0V0 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111441131 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.0e-106
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPKRTAEEDELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match: A0A1S3BQS1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103492284 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.8e-103
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match: A0A5D3CFX2 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002710 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.8e-103
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match: A0A0A0LWC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G575160 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.8e-103
Identity = 216/220 (98.18%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MSMFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQES+ALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGA+ADFDEDELEAELEELE AELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQPA 180

Query: 181 TTAPAAPIPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           TTAPAAPIPVPAGRQTARPAP KRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

[Close]
BLAST of Carg12258 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 335.9 bits (860), Expect = 2.5e-92
Identity = 188/219 (85.84%), Postives = 203/219 (92.69%), Query Frame = 0 

Query: 3   MFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE++AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-IPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           AP  P +PVPAGRQ ARP P KRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 335.9 bits (860), Expect = 2.5e-92
Identity = 188/219 (85.84%), Postives = 203/219 (92.69%), Query Frame = 0 

Query: 3   MFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE++AL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GA+ADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-IPVPAGRQTARPAP-KRTAEEDELAALQAEMAL 220
           AP  P +PVPAGRQ ARP P KRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

[Close]
BLAST of Carg12258 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-85
Identity = 183/217 (84.33%), Postives = 196/217 (90.32%), Query Frame = 0 

Query: 5   NKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAIQC 64
           N+LFGKPKQE+S L TLDKLNETLEMLEKKE VLLKKA+ EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 65  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 124
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 125 DKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATTAP 184
           DKTMDEINEQTENMKQIQEALS P GA+ DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFGAN-DFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 185 AAPIPVPAGRQTAR-PAPKR-TAEEDELAALQAEMAL 220
             PI VP G + AR PA K+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

[Close]
BLAST of Carg12258 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 293.5 bits (750), Expect = 1.4e-79
Identity = 166/192 (86.46%), Postives = 178/192 (92.71%), Query Frame = 0 

Query: 30  MLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 89
           MLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 90  ILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPI 149
           I+LEGAKATTETVDALR+GA+AMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 150 GASADFDEDELEAELEELEGAELEEQLLQPATTAPAAP-IPVPAGRQTARPAP-KRTAEE 209
           GA+ADFDEDEL AEL+ELE  ELE QLLQPATTAP  P +PVPAGRQ ARP P KRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 210 DELAALQAEMAL 220
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

[Close]
BLAST of Carg12258 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 51.6 bits (122), Expect = 9.3e-07
Identity = 57/216 (26.39%), Postives = 91/216 (42.13%), Query Frame = 0 

Query: 3   MFNKLFGKPKQESSALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRAAI 62
           M N    KPK      A L  L      L + ++ L K    E + A+D  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G  A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGASADFDEDELEAELEELEGAELEEQLLQPATT 182
           DV K MD+  E      ++   L   + A    DE+E+ AE + LE   + E + +  TT
Sbjct: 121 DVQKLMDDTAEAKAYQDELSAILGEKLSAE---DEEEILAEFDNLESLLIVEDMPEVPTT 180

Query: 183 -----------APAAPIPVPAGRQTARPAPKRTAEE 208
                       P  P   P          ++  EE
Sbjct: 181 ELMPEEPEKMDLPDVPTKAPVASNETTSTKRKVLEE 212

The following BLAST results are available for this feature:
BLAST of Carg12258 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022933834.16.3e-106100.00vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
XP_008450809.13.8e-10398.18PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
XP_004135686.13.8e-10398.18vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
XP_022961241.11.9e-10297.27vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
XP_038880238.14.2e-10297.27vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida][more]
BLAST of Carg12258 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SZE43.5e-9185.84Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821975.4e-8484.33Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC48.7e-2642.01Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q5XGW61.9e-2541.78Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Q6GL113.3e-2541.78Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b P... [more]
BLAST of Carg12258 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KXI23.0e-106100.00vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxim... [more]
A0A6J1F0V03.0e-106100.00vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A1S3BQS11.8e-10398.18vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5D3CFX21.8e-10398.18Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
A0A0A0LWC61.8e-10398.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G575160 PE=4 SV=1[more]
BLAST of Carg12258 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G29160.12.5e-9285.84SNF7 family protein [more]
AT4G29160.32.5e-9285.84SNF7 family protein [more]
AT2G19830.13.8e-8584.33SNF7 family protein [more]
AT4G29160.21.4e-7986.46SNF7 family protein [more]
AT5G09260.19.3e-0726.39vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..177
NoneNo IPR availableCOILSCoilCoilcoord: 124..144
NoneNo IPR availableCOILSCoilCoilcoord: 14..41
NoneNo IPR availableGENE3D6.10.250.1710coord: 119..174
e-value: 5.1E-9
score: 37.9
NoneNo IPR availableGENE3D1.10.287.1060coord: 16..93
e-value: 2.4E-21
score: 77.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 177..219
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 3..219
NoneNo IPR availablePANTHERPTHR22761:SF53VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 2-LIKEcoord: 3..219
IPR005024Snf7 familyPFAMPF03357Snf7coord: 22..187
e-value: 4.8E-46
score: 156.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12258-RACarg12258-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body