Carg12250 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12250
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	(1->3)-beta-glucan endohydrolase
Location	Carg_Chr11: 2930164 .. 2932090 (+)
RNA-Seq Expression	Carg12250
Synteny	Carg12250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGTGGCTTTTTAAGCTAATTTTGCTCTGTTTTAGCCTTAAAACCATAATCTCGAGCTCTGTAATCCTCACAGTGGGTGCCTTTTTAGAAAGCAAAACGAGCCGTTGCTGTTCTAAACCAGAATCTTACAAATCCCTCGTTCCCATTTCTCTACGCAAGCCCCGCAGCAAAGTTGGACTGAGACTTGAAGGCGTGGATTTCAGGTGATAACGAGAAAGGAGAATGGGGAAAAGGGCTGGATTCAATCTCATTTTCTATGGGTTTTCACTGCTGCTTATGTTCTTGGGTTTGTAGCTCTCTGTTTCTTCTTCGTGTTCTTCATTTTTGCTGTTCTGTTCACTGTTCTTGCTGTTCTGGGTGTGTTCCTCTTCGATTTGGTACCACATTTGACATTTCTTGCGGCGGTTTTGTCTGATTCTACTTGTTGGATCTTCCATTGGCATTGTTGCTACTTATTTAGCTAAACTGAAGTAAACTAATCATTATTCAACCTATTTAGTAACATATTTCTGTGCTTGTATTGATACAAATCATAGATTACAGAATAAAAGATTTGAAACCCTTTGTAAGAAAGGAAAGAACAGACCCTTTTGGTAGAAATCACCATTGTCTTCGTTTTCATGCTTGAAATAAGGCTGTATGTTTCCTGCAGGCCATTGCCAGGGAAGTAATGTTGGAATTTGCTATGGAAGAAATGCAGATGACCTCCCAACACCAAATAAAGTGGCCCAATTGGTTCAACTTCATAACATCAAATACCTTAGAATTTATGACTCAAACATTCAGGTTCTCAAGGCCTTTGCCAACACCGGTGTCGAACTTATGATCGGGATACCGAATTCCGATTTGCTGCCATTCGCTCAGTTTCAGTCTAATGTAGATACTTGGCTGAAGAATAGCATTCTCCCTTACTACCCAGCGGCGAAAATAACATACATTACGGTCGGTGCCGAGGTCACGGAGAGCCCTCACAATGTGTCTGCCTTGGTGGTGCCTGCCATGAACAATGTACTCACTGGTCTTAAGAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCGCTCGGAGTTTTGTCTCGGTCGTTCCCTCCCTCGGCTGGCGCTTTTAGTAGTGATTTTGCATTCTTCTTGAAACCATTGTTGGAGTTTCTTGCTGAGAATCAGTCTCCTTTTATGATCAATATTTATCCTTATTATGCTTACAGAGAATCCCCTAGCAATGTGTCATTGGACTATGCTCTTTTCGAATCGTCGTCTGAAGTTATCGATCCGAACACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCACCAAAGGAGACAGCTGCAACTCCCGACAATGCGCAAACTTACAACACGAATCTTATCCGACATGTTATTAATAACACCGGGACGCCTGCAAGGCCAGGGGAGGAACTGGATGTATATATCTTCTCTTTGTTTAATGAAAACAGGAAGCCAGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGATTTTACAGGAAGAAGTTCCGTAGACATGACTTCACAGGCTAATATGACTGGTTCTAATGGAACCGACTGGTGCATTGCTTCGAGCAAGGCAACCGATATGGACTTGCAAAACGCGTTGGATTGGGCTTGTAGTTCGGGGAATGTCGATTGCACTGCCATCCAGCCAAGCCAGCCTTGTTTTGAGCCAGATACTCTTGTTTCTCATGCATCTTATGCGTTTAACAGTTATTTCCAGCAGAATGGAGCTACTGATGTTGCTTGTGGCTTTGGAGGGAATGGAGTTAAAGTTAGCCAGGATCCA

mRNA sequence

AGAGTGGCTTTTTAAGCTAATTTTGCTCTGTTTTAGCCTTAAAACCATAATCTCGAGCTCTGTAATCCTCACAGTGGGTGCCTTTTTAGAAAGCAAAACGAGCCGTTGCTGTTCTAAACCAGAATCTTACAAATCCCTCGTTCCCATTTCTCTACGCAAGCCCCGCAGCAAAGTTGGACTGAGACTTGAAGGCGTGGATTTCAGGTGATAACGAGAAAGGAGAATGGGGAAAAGGGCTGGATTCAATCTCATTTTCTATGGGTTTTCACTGCTGCTTATGTTCTTGGGCCATTGCCAGGGAAGTAATGTTGGAATTTGCTATGGAAGAAATGCAGATGACCTCCCAACACCAAATAAAGTGGCCCAATTGGTTCAACTTCATAACATCAAATACCTTAGAATTTATGACTCAAACATTCAGGTTCTCAAGGCCTTTGCCAACACCGGTGTCGAACTTATGATCGGGATACCGAATTCCGATTTGCTGCCATTCGCTCAGTTTCAGTCTAATGTAGATACTTGGCTGAAGAATAGCATTCTCCCTTACTACCCAGCGGCGAAAATAACATACATTACGGTCGGTGCCGAGGTCACGGAGAGCCCTCACAATGTGTCTGCCTTGGTGGTGCCTGCCATGAACAATGTACTCACTGGTCTTAAGAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCGCTCGGAGTTTTGTCTCGGTCGTTCCCTCCCTCGGCTGGCGCTTTTAGTAGTGATTTTGCATTCTTCTTGAAACCATTGTTGGAGTTTCTTGCTGAGAATCAGTCTCCTTTTATGATCAATATTTATCCTTATTATGCTTACAGAGAATCCCCTAGCAATGTGTCATTGGACTATGCTCTTTTCGAATCGTCGTCTGAAGTTATCGATCCGAACACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCACCAAAGGAGACAGCTGCAACTCCCGACAATGCGCAAACTTACAACACGAATCTTATCCGACATGTTATTAATAACACCGGGACGCCTGCAAGGCCAGGGGAGGAACTGGATGTATATATCTTCTCTTTGTTTAATGAAAACAGGAAGCCAGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGATTTTACAGGAAGAAGTTCCGTAGACATGACTTCACAGGCTAATATGACTGGTTCTAATGGAACCGACTGGTGCATTGCTTCGAGCAAGGCAACCGATATGGACTTGCAAAACGCGTTGGATTGGGCTTGTAGTTCGGGGAATGTCGATTGCACTGCCATCCAGCCAAGCCAGCCTTGTTTTGAGCCAGATACTCTTGTTTCTCATGCATCTTATGCGTTTAACAGTTATTTCCAGCAGAATGGAGCTACTGATGTTGCTTGTGGCTTTGGAGGGAATGGAGTTAAAGTTAGCCAGGATCCA

Coding sequence (CDS)

ATGGGGAAAAGGGCTGGATTCAATCTCATTTTCTATGGGTTTTCACTGCTGCTTATGTTCTTGGGCCATTGCCAGGGAAGTAATGTTGGAATTTGCTATGGAAGAAATGCAGATGACCTCCCAACACCAAATAAAGTGGCCCAATTGGTTCAACTTCATAACATCAAATACCTTAGAATTTATGACTCAAACATTCAGGTTCTCAAGGCCTTTGCCAACACCGGTGTCGAACTTATGATCGGGATACCGAATTCCGATTTGCTGCCATTCGCTCAGTTTCAGTCTAATGTAGATACTTGGCTGAAGAATAGCATTCTCCCTTACTACCCAGCGGCGAAAATAACATACATTACGGTCGGTGCCGAGGTCACGGAGAGCCCTCACAATGTGTCTGCCTTGGTGGTGCCTGCCATGAACAATGTACTCACTGGTCTTAAGAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCGCTCGGAGTTTTGTCTCGGTCGTTCCCTCCCTCGGCTGGCGCTTTTAGTAGTGATTTTGCATTCTTCTTGAAACCATTGTTGGAGTTTCTTGCTGAGAATCAGTCTCCTTTTATGATCAATATTTATCCTTATTATGCTTACAGAGAATCCCCTAGCAATGTGTCATTGGACTATGCTCTTTTCGAATCGTCGTCTGAAGTTATCGATCCGAACACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCACCAAAGGAGACAGCTGCAACTCCCGACAATGCGCAAACTTACAACACGAATCTTATCCGACATGTTATTAATAACACCGGGACGCCTGCAAGGCCAGGGGAGGAACTGGATGTATATATCTTCTCTTTGTTTAATGAAAACAGGAAGCCAGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGATTTTACAGGAAGAAGTTCCGTAGACATGACTTCACAGGCTAATATGACTGGTTCTAATGGAACCGACTGGTGCATTGCTTCGAGCAAGGCAACCGATATGGACTTGCAAAACGCGTTGGATTGGGCTTGTAGTTCGGGGAATGTCGATTGCACTGCCATCCAGCCAAGCCAGCCTTGTTTTGAGCCAGATACTCTTGTTTCTCATGCATCTTATGCGTTTAACAGTTATTTCCAGCAGAATGGAGCTACTGATGTTGCTTGTGGCTTTGGAGGGAATGGAGTTAAAGTTAGCCAGGATCCA

Protein sequence

MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVKVSQDP

Homology

[Close]

BLAST of Carg12250 vs. NCBI nr
Match: KAG7021902.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. NCBI nr
Match: KAG6588008.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904.0 bits (2335), Expect = 5.0e-259
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. NCBI nr
Match: XP_022931751.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata])

HSP 1 Score: 902.9 bits (2332), Expect = 1.1e-258
Identity = 445/446 (99.78%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. NCBI nr
Match: XP_023531298.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 900.6 bits (2326), Expect = 5.5e-258
Identity = 444/446 (99.55%), Postives = 445/446 (99.78%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKRAGFNLIFYG SLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGKRAGFNLIFYGISLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. NCBI nr
Match: XP_023005326.1 (glucan endo-1,3-beta-glucosidase 13-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 899.4 bits (2323), Expect = 1.2e-257
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG RAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGHRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWL+NSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLRNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.4e-192
Identity = 329/444 (74.10%), Postives = 383/444 (86.26%), Query Frame = 0


Query: 7   FNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQ 66
           F LIF   S+LL+ L  C G  VG+CYGR+ADDLPTP+KV QL+Q HNIKY+RIYD N Q
Sbjct: 5   FKLIF-SISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQ 64

Query: 67  VLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTES 126
           VLKAF NT +ELMIG+PNSDL  F+Q QSNVDTWLKNS+LPYYP  KITYITVGAE T+ 
Sbjct: 65  VLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDD 124

Query: 127 PH-NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLK 186
           PH N S+ VVPAM NVLT L+K GL ++IKVS+T SLG+LSRSFPPSAGAF+S +A+FL+
Sbjct: 125 PHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLR 184

Query: 187 PLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQI 246
           P+LEFLAEN+SPFMI++YPYYAYR+SP+NVSLDY LFESSSEVIDPNTGLLY NMFDAQ+
Sbjct: 185 PMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQV 244

Query: 247 DALYFALMALNFRTIRVMVTETGWPSKGSPKE-TAATPDNAQTYNTNLIRHVINNTGTPA 306
           DALY+AL ALNFRTI++MVTETGWP+KGSPKE  AA+ DNA+TYN+N+IRHV+ N GTPA
Sbjct: 245 DALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPA 304

Query: 307 RPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGS 366
           +PGE ++VYIFSLFNENRK GLDSERNWGLFYPDQTSVY LDFTG+S+    S ++ T S
Sbjct: 305 KPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSN-GFHSNSSGTNS 364

Query: 367 NGT--DWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSY 426
           +G+   WCIASSKA++ DL+ ALDWAC  GNVDCTAIQPSQPCF+PDTLVSHAS+ FNSY
Sbjct: 365 SGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSY 424

Query: 427 FQQNGATDVACGFGGNGVKVSQDP 447
           FQQN ATDVAC FGG GVKV++DP
Sbjct: 425 FQQNRATDVACSFGGAGVKVNKDP 446

[Close]

BLAST of Carg12250 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 8.4e-177
Identity = 298/470 (63.40%), Postives = 376/470 (80.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG+R   NL+F+ F  +L FL     S +GICYGRNAD+LP+PN+V++L+Q  NIK++RI
Sbjct: 1   MGQR--LNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YD+NI VLKAFANTG+ELMIG+PN+DLL FAQFQSNVDTWL N+ILPYYP+ KIT I+VG
Sbjct: 61  YDANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
            EVTE+P N + LV+PAM N+ T LKK+GL KKIK+SS+HSL +LSRSFPPS+ +FS   
Sbjct: 121 LEVTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKH 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           + FLKP+LEFL EN+SPFMI++YPYYAYR+S   V L+YALFESSS+V+DP TGLLY+NM
Sbjct: 181 SAFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQ+DA+YFAL A++F+T++VMVTE+GWPSKGSPKETAATP+NA  YNTNLIRHVI + 
Sbjct: 241 FDAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPA+PGEE+DVY+FSLFNENRKPG++SERNWG+FY + T+VY LDFTG ++  ++   +
Sbjct: 301 GTPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNS 360

Query: 361 MTGS----------NGTD--------------WCIASSKATDMDLQNALDWACSSGNVDC 420
            TG+          NG                WCIASS+A+  +LQ ALDWAC  GNVDC
Sbjct: 361 TTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDC 420

Query: 421 TAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVKVSQDP 447
           +A+QP QPCFEPDT++SHASYAFN+Y+QQ+GA+ + C F G  V+V +DP
Sbjct: 421 SAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDP 468

[Close]

BLAST of Carg12250 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 189/441 (42.86%), Postives = 272/441 (61.68%), Query Frame = 0


Query: 15  SLLLMFL------GHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVL 74
           +LLL+FL         Q S +G+  G    ++P+P +V  L++  NI  +R+YD++  +L
Sbjct: 3   ALLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSML 62

Query: 75  KAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPH 134
            AFA+TGV+++I +PN  LL  +Q  +    W+  ++  YYPA  IT I VG+EV  S  
Sbjct: 63  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLT 122

Query: 135 NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLKPLL 194
           N ++++V A+  +   L  A L ++IKVS+ HS  ++  SFPPS   F+  +   + PLL
Sbjct: 123 NAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLL 182

Query: 195 EFLAENQSPFMINIYPYYAYRESPSNVSLDYALF---ESSSEVIDPNTGLLYTNMFDAQI 254
           +FL    SP ++N+YPY+ Y +S   + LDYALF   +++ E +D NT L YTN+FDA +
Sbjct: 183 KFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIV 242

Query: 255 DALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPAR 314
           DA YFA+  LNF  I ++VTE+GWPSKG P E  AT +NA TYN+NLI+HVIN TGTP  
Sbjct: 243 DAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKH 302

Query: 315 PGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGSN 374
           PG  +  YI+ L+NE+ +PG  SE+NWGLFY + T VY L   G  ++      N T   
Sbjct: 303 PGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQT--- 362

Query: 375 GTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQ 434
              +CIA  K     LQ ALDWAC  G VDC+A+   + C+EPD +V+H++YAFN+Y+Q+
Sbjct: 363 ---FCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQK 422

Query: 435 NGATDVACGFGGNGVKVSQDP 447
            G    +C F G     + DP
Sbjct: 423 MGKASGSCDFKGVATVTTTDP 437

[Close]

BLAST of Carg12250 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 7.8e-98
Identity = 179/434 (41.24%), Postives = 271/434 (62.44%), Query Frame = 0


Query: 16  LLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTG 75
           LLL  L     + +G+  G +  ++P P+ +  L++   I ++R+YD+N  +LKAFANT 
Sbjct: 13  LLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTS 72

Query: 76  VELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPHNVSALVV 135
           +E+M+G+ N ++L   +F S    W+  ++  Y P+  IT I VG+EV  +  +V+ ++ 
Sbjct: 73  IEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILA 132

Query: 136 PAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLKPLLEFLAENQ 195
            A+NN+   L  + L+ K+KVSS  S+ ++ + FPPS   FS  +   +  LL+FL    
Sbjct: 133 SALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTG 192

Query: 196 SPFMINIYPYYAYRESPSNVSLDYALFESSS---EVIDPNTGLLYTNMFDAQIDALYFAL 255
           S FM+N YPYY Y  +     LDYALF+  S   +++DPNT L Y +MFDA +DA Y+++
Sbjct: 193 SFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSM 252

Query: 256 MALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPARPGEELDV 315
            ALNF  I V+VTETGWPS G   E AAT  NA+T+NTNLI+ V+NN+G P++P   ++ 
Sbjct: 253 EALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINT 312

Query: 316 YIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGSNGTDWCIA 375
           YI+ L+NE+++ G  SERNWG+ +P+ TSVY L  +G S     S A + GS  + +C+A
Sbjct: 313 YIYELYNEDKRSGPVSERNWGILFPNGTSVYPLSLSGGS-----SSAALNGS--SMFCVA 372

Query: 376 SSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVA 435
            + A D  L + L+WAC  G  +C AIQP QPC+ P+ + SHAS+AFN Y+Q+  +    
Sbjct: 373 KADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGT 432

Query: 436 CGFGGNGVKVSQDP 447
           C F G  +  ++DP
Sbjct: 433 CDFDGTAITTTRDP 439

[Close]

BLAST of Carg12250 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 358.2 bits (918), Expect = 1.3e-97
Identity = 186/436 (42.66%), Postives = 275/436 (63.07%), Query Frame = 0


Query: 17  LLMFLGHCQGSN----VGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFA 76
           LL+FL H   S+    +G+ YG+ AD+LP P++  +L+Q  +I+ +R+Y ++  ++KA A
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALA 69

Query: 77  NTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEV--TESPHNV 136
            TGV ++IG  N D+   A   +    W+ +++LP+YPA+KI  ITVG E+  +  P+ V
Sbjct: 70  GTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLV 129

Query: 137 SALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLKPLLEF 196
           + L +PAM NV   L+   L  KIKVS+ +S+ VL  S PPS+G+F++ +   LK +L+F
Sbjct: 130 NQL-LPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQF 189

Query: 197 LAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYF 256
           L++  SPF IN YP++AY+  P   +L + LFE ++  +D  TG+ YTNMFDAQ+DA++ 
Sbjct: 190 LSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHS 249

Query: 257 ALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPARPGEEL 316
           AL ++ F  + ++V ETGW S+G   E  A+ DNA+ YN NLI H+ +  GTP  PG+ +
Sbjct: 250 ALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPV 309

Query: 317 DVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGSNGTDWC 376
           D YIF+L++EN KPG  SER +GLF  D + VY++     SS   T    +T S    WC
Sbjct: 310 DTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGKVTSSG---WC 369

Query: 377 IASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATD 436
           +    AT+ +LQ +LDWAC  G +DC AIQP   CFEP+ +VSHA+YA N YFQ++    
Sbjct: 370 VPKKGATNEELQASLDWACGHG-IDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQP 429

Query: 437 VACGFGGNGVKVSQDP 447
             C F       SQ+P
Sbjct: 430 TDCDFSKTATVTSQNP 440

[Close]

BLAST of Carg12250 vs. ExPASy TrEMBL
Match: A0A6J1EUI9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438017 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.3e-259
Identity = 445/446 (99.78%), Postives = 446/446 (100.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. ExPASy TrEMBL
Match: A0A6J1KYV7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498355 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 5.9e-258
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG RAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGHRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWL+NSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLRNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. ExPASy TrEMBL
Match: A0A6J1KUN1 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498355 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 5.9e-258
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG RAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI
Sbjct: 1   MGHRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWL+NSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLRNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF
Sbjct: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRS+VDMTSQAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSAVDMTSQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKVSQDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVSQDP 446

[Close]

BLAST of Carg12250 vs. ExPASy TrEMBL
Match: A0A6J1CTD8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014572 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.2e-244
Identity = 421/446 (94.39%), Postives = 433/446 (97.09%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG  A FNLIF GFSL L+ LGHCQGSNVG+CYGRNADDLPTPNKVAQLVQLHNIKYLR+
Sbjct: 1   MGGGAEFNLIF-GFSLFLILLGHCQGSNVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRL 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIGIPNSDLLPF+QFQSNVDTWLKNSILPYYPAAKITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFSQFQSNVDTWLKNSILPYYPAAKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESP+NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSS++
Sbjct: 121 AEVTESPNNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNY 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM
Sbjct: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLI HVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIHHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDF GR +VDMT+QAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFAGRGAVDMTTQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
           +TGSNGT WCIASSKA+DMDLQNALDWAC  GNVDCTAIQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 VTGSNGTAWCIASSKASDMDLQNALDWACGPGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKV +DP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVDKDP 445

[Close]

BLAST of Carg12250 vs. ExPASy TrEMBL
Match: A0A0A0M1T0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G575070 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.7e-244
Identity = 416/446 (93.27%), Postives = 432/446 (96.86%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MGKR GFNLIF G SL L+ LGHCQGSNVG+CYGRNADDLPTPNKVAQLV+LHNIKY+RI
Sbjct: 1   MGKRVGFNLIFLGISLFLILLGHCQGSNVGVCYGRNADDLPTPNKVAQLVKLHNIKYIRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YDSNIQVLKAFANTGVELMIG+PNSDLLPFAQFQSNVDTWLKNSILPYYPA KITYITVG
Sbjct: 61  YDSNIQVLKAFANTGVELMIGVPNSDLLPFAQFQSNVDTWLKNSILPYYPATKITYITVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
           AEVTESP+NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPS GAFSS++
Sbjct: 121 AEVTESPNNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSVGAFSSNY 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           A FLKPLLEFLAENQSPFMINIYPYYAYRESP+NVSLDYALFESS+EVIDPNTGLLYTNM
Sbjct: 181 ASFLKPLLEFLAENQSPFMINIYPYYAYRESPNNVSLDYALFESSNEVIDPNTGLLYTNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKET+ATPDNAQTYNTNLIRHVINNT
Sbjct: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNT 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTG+S VDMT+QAN
Sbjct: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGKSVVDMTAQAN 360

Query: 361 MTGSNGTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFN 420
            T SNGT WCIAS+KA+DMDLQNALDWAC SGNVDCT IQPSQPCFEPDTLVSHASYAFN
Sbjct: 361 STASNGTAWCIASTKASDMDLQNALDWACGSGNVDCTPIQPSQPCFEPDTLVSHASYAFN 420

Query: 421 SYFQQNGATDVACGFGGNGVKVSQDP 447
           SYFQQNGATDVACGFGGNGVKV+QDP
Sbjct: 421 SYFQQNGATDVACGFGGNGVKVNQDP 446

[Close]

BLAST of Carg12250 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.0e-193
Identity = 329/444 (74.10%), Postives = 383/444 (86.26%), Query Frame = 0


Query: 7   FNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQ 66
           F LIF   S+LL+ L  C G  VG+CYGR+ADDLPTP+KV QL+Q HNIKY+RIYD N Q
Sbjct: 5   FKLIF-SISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQ 64

Query: 67  VLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTES 126
           VLKAF NT +ELMIG+PNSDL  F+Q QSNVDTWLKNS+LPYYP  KITYITVGAE T+ 
Sbjct: 65  VLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDD 124

Query: 127 PH-NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLK 186
           PH N S+ VVPAM NVLT L+K GL ++IKVS+T SLG+LSRSFPPSAGAF+S +A+FL+
Sbjct: 125 PHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLR 184

Query: 187 PLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQI 246
           P+LEFLAEN+SPFMI++YPYYAYR+SP+NVSLDY LFESSSEVIDPNTGLLY NMFDAQ+
Sbjct: 185 PMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQV 244

Query: 247 DALYFALMALNFRTIRVMVTETGWPSKGSPKE-TAATPDNAQTYNTNLIRHVINNTGTPA 306
           DALY+AL ALNFRTI++MVTETGWP+KGSPKE  AA+ DNA+TYN+N+IRHV+ N GTPA
Sbjct: 245 DALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPA 304

Query: 307 RPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGS 366
           +PGE ++VYIFSLFNENRK GLDSERNWGLFYPDQTSVY LDFTG+S+    S ++ T S
Sbjct: 305 KPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSN-GFHSNSSGTNS 364

Query: 367 NGT--DWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSY 426
           +G+   WCIASSKA++ DL+ ALDWAC  GNVDCTAIQPSQPCF+PDTLVSHAS+ FNSY
Sbjct: 365 SGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSY 424

Query: 427 FQQNGATDVACGFGGNGVKVSQDP 447
           FQQN ATDVAC FGG GVKV++DP
Sbjct: 425 FQQNRATDVACSFGGAGVKVNKDP 446

[Close]

BLAST of Carg12250 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 621.3 bits (1601), Expect = 6.0e-178
Identity = 298/470 (63.40%), Postives = 376/470 (80.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG+R   NL+F+ F  +L FL     S +GICYGRNAD+LP+PN+V++L+Q  NIK++RI
Sbjct: 1   MGQR--LNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YD+NI VLKAFANTG+ELMIG+PN+DLL FAQFQSNVDTWL N+ILPYYP+ KIT I+VG
Sbjct: 61  YDANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
            EVTE+P N + LV+PAM N+ T LKK+GL KKIK+SS+HSL +LSRSFPPS+ +FS   
Sbjct: 121 LEVTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKH 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           + FLKP+LEFL EN+SPFMI++YPYYAYR+S   V L+YALFESSS+V+DP TGLLY+NM
Sbjct: 181 SAFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQ+DA+YFAL A++F+T++VMVTE+GWPSKGSPKETAATP+NA  YNTNLIRHVI + 
Sbjct: 241 FDAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPA+PGEE+DVY+FSLFNENRKPG++SERNWG+FY + T+VY LDFTG ++  ++   +
Sbjct: 301 GTPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNS 360

Query: 361 MTGS----------NGTD--------------WCIASSKATDMDLQNALDWACSSGNVDC 420
            TG+          NG                WCIASS+A+  +LQ ALDWAC  GNVDC
Sbjct: 361 TTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDC 420

Query: 421 TAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVKVSQDP 447
           +A+QP QPCFEPDT++SHASYAFN+Y+QQ+GA+ + C F G  V+V +DP
Sbjct: 421 SAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDP 468

[Close]

BLAST of Carg12250 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 621.3 bits (1601), Expect = 6.0e-178
Identity = 298/470 (63.40%), Postives = 376/470 (80.00%), Query Frame = 0


Query: 1   MGKRAGFNLIFYGFSLLLMFLGHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRI 60
           MG+R   NL+F+ F  +L FL     S +GICYGRNAD+LP+PN+V++L+Q  NIK++RI
Sbjct: 1   MGQR--LNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRI 60

Query: 61  YDSNIQVLKAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVG 120
           YD+NI VLKAFANTG+ELMIG+PN+DLL FAQFQSNVDTWL N+ILPYYP+ KIT I+VG
Sbjct: 61  YDANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVG 120

Query: 121 AEVTESPHNVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDF 180
            EVTE+P N + LV+PAM N+ T LKK+GL KKIK+SS+HSL +LSRSFPPS+ +FS   
Sbjct: 121 LEVTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKH 180

Query: 181 AFFLKPLLEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNM 240
           + FLKP+LEFL EN+SPFMI++YPYYAYR+S   V L+YALFESSS+V+DP TGLLY+NM
Sbjct: 181 SAFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNM 240

Query: 241 FDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNT 300
           FDAQ+DA+YFAL A++F+T++VMVTE+GWPSKGSPKETAATP+NA  YNTNLIRHVI + 
Sbjct: 241 FDAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP 300

Query: 301 GTPARPGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQAN 360
           GTPA+PGEE+DVY+FSLFNENRKPG++SERNWG+FY + T+VY LDFTG ++  ++   +
Sbjct: 301 GTPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNS 360

Query: 361 MTGS----------NGTD--------------WCIASSKATDMDLQNALDWACSSGNVDC 420
            TG+          NG                WCIASS+A+  +LQ ALDWAC  GNVDC
Sbjct: 361 TTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDC 420

Query: 421 TAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVKVSQDP 447
           +A+QP QPCFEPDT++SHASYAFN+Y+QQ+GA+ + C F G  V+V +DP
Sbjct: 421 SAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDP 468

[Close]

BLAST of Carg12250 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.2e-103
Identity = 189/441 (42.86%), Postives = 272/441 (61.68%), Query Frame = 0


Query: 15  SLLLMFL------GHCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVL 74
           +LLL+FL         Q S +G+  G    ++P+P +V  L++  NI  +R+YD++  +L
Sbjct: 3   ALLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSML 62

Query: 75  KAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPH 134
            AFA+TGV+++I +PN  LL  +Q  +    W+  ++  YYPA  IT I VG+EV  S  
Sbjct: 63  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLT 122

Query: 135 NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAFFLKPLL 194
           N ++++V A+  +   L  A L ++IKVS+ HS  ++  SFPPS   F+  +   + PLL
Sbjct: 123 NAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLL 182

Query: 195 EFLAENQSPFMINIYPYYAYRESPSNVSLDYALF---ESSSEVIDPNTGLLYTNMFDAQI 254
           +FL    SP ++N+YPY+ Y +S   + LDYALF   +++ E +D NT L YTN+FDA +
Sbjct: 183 KFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIV 242

Query: 255 DALYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPAR 314
           DA YFA+  LNF  I ++VTE+GWPSKG P E  AT +NA TYN+NLI+HVIN TGTP  
Sbjct: 243 DAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKH 302

Query: 315 PGEELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANMTGSN 374
           PG  +  YI+ L+NE+ +PG  SE+NWGLFY + T VY L   G  ++      N T   
Sbjct: 303 PGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQT--- 362

Query: 375 GTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQ 434
              +CIA  K     LQ ALDWAC  G VDC+A+   + C+EPD +V+H++YAFN+Y+Q+
Sbjct: 363 ---FCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQK 422

Query: 435 NGATDVACGFGGNGVKVSQDP 447
            G    +C F G     + DP
Sbjct: 423 MGKASGSCDFKGVATVTTTDP 437

[Close]

BLAST of Carg12250 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.2e-103
Identity = 192/441 (43.54%), Postives = 272/441 (61.68%), Query Frame = 0


Query: 11  FYGFSLLLMFLG--HCQGSNVGICYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVL 70
           F  + L+L FL         VG+ YGR A++LP+P KV  L++   I  ++I+D++  VL
Sbjct: 5   FLPYFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVL 64

Query: 71  KAFANTGVELMIGIPNSDLLPFAQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPH 130
            A AN+ +++++ +PN  L   A  QS  D W+K  I+PY+PA +I  I VG EV   P 
Sbjct: 65  TALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP- 124

Query: 131 NVSALVVPAMNNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSDFAF-FLKPL 190
            ++  +V AM N+ T L K  L K IK+SS  +L  L+ S+PPS+G+F  +     +KP+
Sbjct: 125 TITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPM 184

Query: 191 LEFLAENQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDA 250
           L  L +  S  M+N YP++AY  +   +SLDYALF+ ++  ID  TGL Y ++FDAQIDA
Sbjct: 185 LALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDA 244

Query: 251 LYFALMALNFRTIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIRHVINNTGTPARPG 310
           +Y AL A+ F+ ++VMVTETGWPS G   E  A+  NA  YN  L++ V+   GTP RP 
Sbjct: 245 VYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPT 304

Query: 311 EELDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGRSSVDMTSQANM--TGSN 370
           E L+VY+F+LFNEN+KPG  SERN+GLFYP++  VYN+ FT +S+  +            
Sbjct: 305 EPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKKSTTPVNGNRGKVPVTHE 364

Query: 371 GTDWCIASSKATDMDLQNALDWACSSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQ 430
           G  WC+++ +     LQ ALD+AC  G  DC  IQP   C+ P++L +HASYAFNSY+Q+
Sbjct: 365 GHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHASYAFNSYYQK 424

Query: 431 NGATDVACGFGGNGVKVSQDP 447
           N      C FGG    V+Q P
Sbjct: 425 NSRRVGTCFFGGAAHVVTQPP 444

The following BLAST results are available for this feature:

BLAST of Carg12250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021902.1	5.0e-259	100.00	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argy...	[more]
KAG6588008.1	5.0e-259	100.00	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022931751.1	1.1e-258	99.78	glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata]	[more]
XP_023531298.1	5.5e-258	99.55	glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo]	[more]
XP_023005326.1	1.2e-257	99.33	glucan endo-1,3-beta-glucosidase 13-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg12250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJU9	1.4e-192	74.10	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q8VYE5	8.4e-177	63.40	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9ZU91	1.2e-101	42.86	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q94CD8	7.8e-98	41.24	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9M069	1.3e-97	42.66	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of Carg12250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EUI9	5.3e-259	99.78	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438017 P...	[more]
A0A6J1KYV7	5.9e-258	99.33	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498355 PE=...	[more]
A0A6J1KUN1	5.9e-258	99.33	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498355 PE=...	[more]
A0A6J1CTD8	2.2e-244	94.39	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014572 ...	[more]
A0A0A0M1T0	3.7e-244	93.27	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G575070 PE=3...	[more]

BLAST of Carg12250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56590.1	1.0e-193	74.10	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.2	6.0e-178	63.40	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.1	6.0e-178	63.40	O-Glycosyl hydrolases family 17 protein	[more]
AT2G01630.1	8.2e-103	42.86	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	8.2e-103	43.54	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 368..446 e-value: 1.1E-32 score: 124.5
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 369..439 e-value: 2.6E-20 score: 72.9
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 27..354 e-value: 3.0E-126 score: 422.7
None	No IPR available	GENE3D	1.20.58.1040		coord: 364..446 e-value: 1.8E-9 score: 39.8
None	No IPR available	PANTHER	PTHR32227:SF88	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 13	coord: 15..443
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 29..347 e-value: 3.2E-97 score: 325.9
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 260..273
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 15..443
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 29..347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12250-RA	Carg12250-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds