Carg12235 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12235
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Malic enzyme
Location	Carg_Chr11: 2848991 .. 2854622 (+)
RNA-Seq Expression	Carg12235
Synteny	Carg12235

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTTGCTTCTTCTTTCTGCCACTCTCTTTGCGTGGGGTGCTTCCAATTTGAGTTGAGTGATGAAGAGCACTTTGAAGGAGGTAGGTGATGGCGTTCAGGACATCAATGGAGAACACCGTGCTACAGAGAAGCAGCTCGTCACTCCAAGGACTTCCTCAGTTGCTAGGTATATCCAACTTTCTTGGATTTGGTATAATGGCTTGTGGTTTGACTGATTACTTAACACTCTCAGTTCATCGTTGTATGTAACATAATCCCAACTTATGGGAACAAACTTGCTTTTTATGGTGAGGGGGGAGATTCCAAGTTGGTTGGAGAGGGAATGAAGTCTTTCTTATAGGGGTGTGGAAATCTCTTCTTGACATATGCGTTTTAAAACTGGGAGGTTGACGAAATCTCTTCTTGACATATGCGTTTTAAAACTGGGAGGTTGAACGGGCTGCCAAAGCGGACAATATCTGCTAGTGCTGTCCTCGGGCCGTTACAAATGGTATCGGAAGCATTCTTTACGTGGGTGTGGAAACCTCTCTTTAATAGACGCGTTTTAAAATCGTGAGATTGGCAACGATACGTAACGAGGTAAAGTGAACAATATCGGTTAGCTGTAGGCTTTGGCTGTTACAAATGGTATAAGAGCTAGACACTAAGCAGTGTGCCAGCGAGGACGTTGGGCCCCCAAGGGGGGTGGATTGGGAGATCCCACGTTAGTTAGAGAGGGGAATAAAACAATCCTTGGTGTGGAAATCTCTCCCTAGCAGACGTGTTTTAAGACTGTGAGACTAACGACGATAGATAATGGGCCAAAGTGAACAATATTTGCTAGTGGTGAGCTTGAACTATTACAAATGGTATAAGAGTTAAACATTGAGTGGTGTGCCAACGAGGACTTTGGGCCCCCAAAGAGAGTGGATTGTGAGATCTCACATCGGTTGGAGAGGAGAATGAATCATTCTTTATAAGGGCGTGAAAACAACATTTTTTTATAAGGGTGTGGAAACCTCTTGCTACCAGACGCGTTTTAGAATCGTGAGGCTGACAACGATACGTAACAGACCAAAACGGACAATATCTATTAGTGGTGGGCTTGGACTTTTACATAAAAGTCAACACCGGACTGTGTGTCCCAGCCACCCTAATGAATTACCATCAGGAGCTTTTGATTCTTCCTCTTGATTCTAATCATGCTGGCCTCTGGACCTTTGGCCTTTGAGTTGGATTCTTAGAATATTACTATTCACCGTAAAAGGGACAATGTTGTTCTTAAGATGTAAACATGGATCCTTTTTGCAATCTGGTTTGATGCAGTGGCTATTCCTTGCTGAGGGATCCCCGCCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATCATTTACGGGGGCTTTTGCCGCCTGCCGTTGCTACTCAACAACTTCAGGTTGAGTTTTTTAGTGGTCATGTCTCTGATGTACTCCTTCATGACCTTTTCCATTTGTGATACCAGGAGAAGAGGTTGATGCAGAACATTAGACAATATCAACTTCCACTACAAAAATTTATTGCCATGATTGAACTTCAGGTTCTCACTTTACCTACCATTTGTTTTTCTTCTAAATATGTGCCAATTTTGATTCAATGGAGTTTGAAATTTGGTGTACAGGAAAGAAATGAAAGGCTTTTCTACAAACTTCTTATCGAGAATGTCACAGAACTGCTTCCTATTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAAAAATACGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGTAAGTATGCTTTTCATGACTTCCAGGGCTCTGTGAATCTTATAACATTGAGTCATGCAGGGGTAAGATCGTTGAAGTATTGAAGAATTGGCCCCAAAAGAGCATCCAAGTAATAGTCGTGACTGATGGCGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAAGTAATTTCTATGCAAACATTCTCTGATGAACTAGACCTACTTTCATTCTAGACTGACTGATTTTGATATGAATTTATTGGATGTTTTAGGGAATGGGGATTTCAGTTGGGAAGCTTTCTTTATACGCTGCACTTGGAGGTGTTCGTCCTTCTGCAGTAAGCATCTTACTTGTAAAGCAATACCACGATGTTGTTCATTTCAGCTAATACCACATTTTCTTGTCAGTGTTTGCCTATTGCAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAAAGGTTCAGGAGGCGTCTTTGTGTTGCTTTTGGCTGGTTTAAGTATATTTGATATGGATGCCTATGTTCTATGCAGGAGTACTATGAACTTGTACATGAGTTTATGATGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGGTTAGAATATGTTTGATATACGTTTGATTTGTTTCTGTACTTACTGGGTCTATTTGAGACTTTCAGTTTGAGGATTTTGCCAACCATAATGCTTTCGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTTTTCAATGATGATATTCAGGTACTGAAATTTGATACCCATTATTTGGGAAGTTGTCAAATTTTCTTATCCGTACTCTTGTTCTTGTTTCAGGGGACAGCGTCTGTTGTTCTTGCGGGAGTTGTTTCTGCTCTGAAACTCATTGGTGGTACCTTGGCTGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGTAAGATCTCTCCTTCCATGCTTCCATTCCTCCCTCTACTTCTCTAAATTCCTTGTGTAAATGAGCTACTATAGTGTTGTGAGATACCGCCTCGGTTGGAGAGAGGAACGAAGCATTCTTTATAAAGGTGTGAAAACCTCTCCCTAGTAGACACGTTTTAAAACCATGAGGTTGACGGCAATATGTAACGAACCGAAGCGGACAATATCTACTAGCAGTGGACTTGGGCTGTTACAAATGATATCAAAACCAAACACCGGGCGGTGTGCCAAGAGGATGCTGGCCACTAAGGGGGATGGATTATGAGATTCCACGTCGGTTGGAGTGGGGAACATAGCATTTCCTTATAAGGGTGTGGAAACCTTTCACTAGTAGACACGTTTTAAAACCATGAGGCTGACAACGATACGTAATTGAGCCAAAGCGAATAATAGCTACTAACGGTGGGCTTGGACTGTTACAAATGGTATCAAAGCTAGACACCAGACGATGTGCCAACGAGGATGCTGACTCCTTAGAGGGTGGACTGTGAATCCCACATCGATTGGAGGGAGGAAGGAAACATTCCTTATAAGGGGGTGGAAATCTCTTCCCTAGTAGACGCATTTTAAAACCGTGAAGCTGACAGCGATATGTAACGGGCTAACGGGCTGTTACAAGTATACCAGCCAAGCTGTTACAAGTATCTTTCATTCCCACTCCTTCACATTCATGTTCAAAATGTTCTTACATATTTTTTTGGCGTGGGTGCAGGCAGGAACTGGTATTGCAGAGCTTATAGCTTATGAAGTATCAAGACAGGTACTTGTGAAGCTAAACCAAACATAAACCTATGTTTCTTATAATTCATCTTCTGTTTTTTTTGTTGTTTTAGACAAATGTTCCAATTGAGGAGACCCGCAAGAAGATCTGGCTTGTGGACTCGAAGGTGGTAGAAAGTTTAATGAAGCTTATGATAGAAGAACCATAATTATTTCTTATGTGTTCATGCCATTGCTCTCTTGCTTCAACCATAAACAGAATGATGTTGTTGTAGGGATTAATTGTCCGCTCCCGTAAGGATTCACTTCCACACTTCAAGAAGCCATGGGCTCATGAGCATCAACCTGTTAAGGATCTATTAAGTGCTGTCAAGGTGTGTACCATCTCTTAATCTCGAAATGCTTTGTTGTTGCTCACCTTTTCTCACTATTTCAGGCAATTAAGCCAACCGTTTTGATTGGATCATCTGGAGTTGGAAGAACATTTACGAAAGAAGTGGTCGAGGCTATGTCCTCCATCAATCAGGTTCTTTCTTCTCCCTCCCTCGTTCATGCCTAACCAAATATACATATTGGATACATGTTAAGAATCGGTGATTCCACAGCAAACGCAATAAGAACTCCGGCATAGAATACTTTCAATAGAGAAGATCCGTCGACCTAAGAGGAAGAGAAGAGTTGAGAACTGTGAGATCCCACGTCAGTTAGAGAGGAGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCTATAACAGACGCGTTTTAAAAACATTGAGGGGAACTCCGAAAAGAAAAACCCAAATATGACGATGTCTGCTAGCTGTGGGCTTGAGCTCCGAAGAGGGTGGACACGAGGCGGTGTGCCAACAAGGACACTGAGCCCCGTAGGAGGGTGGATTGAGGGTCCCACTCCGATTGGAGAAGGGAACGAGTGCCAACAAAGACGCTGGCCCTGAAGGAGAATGAATTATGAGATCCTACATCAATTGGAGAGTAGAATGAAGTATACGCATTTTAAAAAACTGGAGAGAAAGCTGAAAGAGACAATATTTAGTACAAGTGGACTGTGGATACAAACTGATATTGTTTTTTGTCATACCATTGTGCAGAAACCTCTTATAATGGCTCTTTCCAACCCCACATCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTAAGGTAATACACATGACATTTTAAAGCCAAAGTTTTTCCTCTCTTTTTCATTGGAACCTCTCTATTTACTTAGCTTCTATATGCAAATTGAAGGGCCGTGCAATCTTTGCTAGCGGAAGTCCTTTCGAGCCATTCAAATACAAGGGGAAAACCTTTGTCCCTGGCCAGGTTTTTCTACTTCTATCATTATGCTGTATGAGTTACTCTTCATTACTGTTGTCCCATACCAAGAGCTGACATATTGAGCTTGTCAAATCTCAAATCAGGCCAACAATGCCTACATATTCCCTGGCTTTGGTTTAGGTATCTTAATTTCCGGAGCAGTTCGCGTGCATGACGACATGCTTCTGGCTGCCGGTAAGTAGGATTGTTTAGTTCTTAACTAATCTTATCTAGTTAGAAACAACAGACATCCACAATAGTAACAACAGGCTCACCGAGCTGACTTGTATCTTATTTCGCCTTTGTCAAACTAGCGGAAGCATTGGCTGCCCAAGTCTCCAAAGAGAACTATGACAAGGGGATGATCTACCCGCCCTTCTCTAGCATCCGAAAGATCTCAGCACACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGTGAGACATTTTCTTTGAATATGTTGCTATTCATGTAGTTTTGAGTACTCTTCCATAACATAAATTACTTAATCTCAAGGCTTGGCAACCCGTCGCCCGCGCCCGGCAGATCTTGTCAAGTATGCTGAGAGCAGCATGTACAGCCCCGTCTACTAAACCGACTGATAAACGTAACGAGATCTCGAGCAGCATGTGTGTTCTTAGGAGAGAACTTGAAGTGTTCCCAAAATCTTTACTTGTAATGGTATAATGTTTGTAATGTTTTTGCTTCATAAGTCAACTTCTTTATTAATGAAATAAGCATTTTGATGAACTTTTACTAA

mRNA sequence

TTTCTTGCTTCTTCTTTCTGCCACTCTCTTTGCGTGGGGTGCTTCCAATTTGAGTTGAGTGATGAAGAGCACTTTGAAGGAGGTAGGTGATGGCGTTCAGGACATCAATGGAGAACACCGTGCTACAGAGAAGCAGCTCGTCACTCCAAGGACTTCCTCAGTTGCTAGTGGCTATTCCTTGCTGAGGGATCCCCGCCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATCATTTACGGGGGCTTTTGCCGCCTGCCGTTGCTACTCAACAACTTCAGGAGAAGAGGTTGATGCAGAACATTAGACAATATCAACTTCCACTACAAAAATTTATTGCCATGATTGAACTTCAGGAAAGAAATGAAAGGCTTTTCTACAAACTTCTTATCGAGAATGTCACAGAACTGCTTCCTATTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAAAAATACGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGTAAGATCGTTGAAGTATTGAAGAATTGGCCCCAAAAGAGCATCCAAGTAATAGTCGTGACTGATGGCGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAAGGAATGGGGATTTCAGTTGGGAAGCTTTCTTTATACGCTGCACTTGGAGGTGTTCGTCCTTCTGCATGTTTGCCTATTGCAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAAAGGAGTACTATGAACTTGTACATGAGTTTATGATGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAGGATTTTGCCAACCATAATGCTTTCGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTTTTCAATGATGATATTCAGGGGACAGCGTCTGTTGTTCTTGCGGGAGTTGTTTCTGCTCTGAAACTCATTGGTGGTACCTTGGCTGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGCAGGAACTGGTATTGCAGAGCTTATAGCTTATGAAGTATCAAGACAGACAAATGTTCCAATTGAGGAGACCCGCAAGAAGATCTGGCTTGTGGACTCGAAGGGATTAATTGTCCGCTCCCGTAAGGATTCACTTCCACACTTCAAGAAGCCATGGGCTCATGAGCATCAACCTGTTAAGGATCTATTAAGTGCTGTCAAGGCAATTAAGCCAACCGTTTTGATTGGATCATCTGGAGTTGGAAGAACATTTACGAAAGAAGTGGTCGAGGCTATGTCCTCCATCAATCAGAAACCTCTTATAATGGCTCTTTCCAACCCCACATCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTAAGGGCCGTGCAATCTTTGCTAGCGGAAGTCCTTTCGAGCCATTCAAATACAAGGGGAAAACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATATTCCCTGGCTTTGGTTTAGGTATCTTAATTTCCGGAGCAGTTCGCGTGCATGACGACATGCTTCTGGCTGCCGCGGAAGCATTGGCTGCCCAAGTCTCCAAAGAGAACTATGACAAGGGGATGATCTACCCGCCCTTCTCTAGCATCCGAAAGATCTCAGCACACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGCTTGGCAACCCGTCGCCCGCGCCCGGCAGATCTTGTCAAGTATGCTGAGAGCAGCATGTACAGCCCCGTCTACTAAACCGACTGATAAACGTAACGAGATCTCGAGCAGCATGTGTGTTCTTAGGAGAGAACTTGAAGTGTTCCCAAAATCTTTACTTGTAATGGTATAATGTTTGTAATGTTTTTGCTTCATAAGTCAACTTCTTTATTAATGAAATAAGCATTTTGATGAACTTTTACTAA

Coding sequence (CDS)

ATGAAGAGCACTTTGAAGGAGGTAGGTGATGGCGTTCAGGACATCAATGGAGAACACCGTGCTACAGAGAAGCAGCTCGTCACTCCAAGGACTTCCTCAGTTGCTAGTGGCTATTCCTTGCTGAGGGATCCCCGCCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATCATTTACGGGGGCTTTTGCCGCCTGCCGTTGCTACTCAACAACTTCAGGAGAAGAGGTTGATGCAGAACATTAGACAATATCAACTTCCACTACAAAAATTTATTGCCATGATTGAACTTCAGGAAAGAAATGAAAGGCTTTTCTACAAACTTCTTATCGAGAATGTCACAGAACTGCTTCCTATTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAAAAATACGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGTAAGATCGTTGAAGTATTGAAGAATTGGCCCCAAAAGAGCATCCAAGTAATAGTCGTGACTGATGGCGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAAGGAATGGGGATTTCAGTTGGGAAGCTTTCTTTATACGCTGCACTTGGAGGTGTTCGTCCTTCTGCATGTTTGCCTATTGCAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAGAAGGGCAACTGGAAAGGAGTACTATGAACTTGTACATGAGTTTATGATGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAGGATTTTGCCAACCATAATGCTTTCGAGCTACTGGCCAAGTATCGCACATCTCATCTAGTTTTCAATGATGATATTCAGGGGACAGCGTCTGTTGTTCTTGCGGGAGTTGTTTCTGCTCTGAAACTCATTGGTGGTACCTTGGCTGATCACACTTTCTTGTTCCTTGGTGCTGGGGAAGCAGGAACTGGTATTGCAGAGCTTATAGCTTATGAAGTATCAAGACAGACAAATGTTCCAATTGAGGAGACCCGCAAGAAGATCTGGCTTGTGGACTCGAAGGGATTAATTGTCCGCTCCCGTAAGGATTCACTTCCACACTTCAAGAAGCCATGGGCTCATGAGCATCAACCTGTTAAGGATCTATTAAGTGCTGTCAAGGCAATTAAGCCAACCGTTTTGATTGGATCATCTGGAGTTGGAAGAACATTTACGAAAGAAGTGGTCGAGGCTATGTCCTCCATCAATCAGAAACCTCTTATAATGGCTCTTTCCAACCCCACATCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTAAGGGCCGTGCAATCTTTGCTAGCGGAAGTCCTTTCGAGCCATTCAAATACAAGGGGAAAACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATATTCCCTGGCTTTGGTTTAGGTATCTTAATTTCCGGAGCAGTTCGCGTGCATGACGACATGCTTCTGGCTGCCGCGGAAGCATTGGCTGCCCAAGTCTCCAAAGAGAACTATGACAAGGGGATGATCTACCCGCCCTTCTCTAGCATCCGAAAGATCTCAGCACACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGCTTGGCAACCCGTCGCCCGCGCCCGGCAGATCTTGTCAAGTATGCTGAGAGCAGCATGTACAGCCCCGTCTACTAA

Protein sequence

MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYELGLATRRPRPADLVKYAESSMYSPVY

Homology

[Close]

BLAST of Carg12235 vs. NCBI nr
Match: KAG7021888.1 (hypothetical protein SDJN02_15616 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL
Sbjct: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLATRRPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. NCBI nr
Match: KAG6587994.1 (hypothetical protein SDJN03_16559, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 568/572 (99.30%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKE GDGVQDING+HRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEEGDGVQDINGQHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYY+L+HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL
Sbjct: 241 KEYYDLLHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLATRRPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. NCBI nr
Match: XP_022930270.1 (NADP-dependent malic enzyme-like [Cucurbita moschata])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 567/572 (99.13%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKE GDGVQDING+HRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEEGDGVQDINGQHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL
Sbjct: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVS+QTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSKQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEV+EAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVIEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPF+PFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLATRRPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. NCBI nr
Match: XP_023006774.1 (NADP-dependent malic enzyme-like [Cucurbita maxima])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 559/572 (97.73%), Postives = 569/572 (99.48%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKE+GDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEIGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVA+QQLQEK+LMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVASQQLQEKKLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYY+L+HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRT H VFNDDIQGTASVVL
Sbjct: 241 KEYYDLLHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTYHPVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVS+QTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSKQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPF+PFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKG+IYPPF++IRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGLIYPPFTNIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLAT RPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATHRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. NCBI nr
Match: XP_023531114.1 (NADP-dependent malic enzyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 558/572 (97.55%), Postives = 566/572 (98.95%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKEVGDGVQDINGEHRATEKQLVTP TSSVASGYSLLRDPR  KGLAFTEKERDAH
Sbjct: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPGTSSVASGYSLLRDPRQYKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVA+QQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLI+NVTELL
Sbjct: 61  HLRGLLPPAVASQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIDNVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYY+L+HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL
Sbjct: 241 KEYYDLLHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGG LADHTFLFLGAGEAGTGIAELIAYEVS+QTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGALADHTFLFLGAGEAGTGIAELIAYEVSKQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEE YTWSKG AIFASGSPF+PFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEVYTWSKGCAIFASGSPFDPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPF++IRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFTNIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLATRRPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 3.5e-276
Identity = 472/565 (83.54%), Postives = 523/565 (92.57%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           VG GV+D+ GE  ATE QLVTP T SVASGYSLLRDPRHNKGLAF +KERDAH+L GLLP
Sbjct: 23  VGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDPRHNKGLAFNDKERDAHYLCGLLP 82

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V+TQ+LQE++LM +IRQYQ+PLQK++AM++LQERNERLFYKLLI+NV ELLP+VYTPT
Sbjct: 83  PVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPT 142

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQKYGSIFRRPQGLYISLKEKGKI+EVLKNWP++ IQVIVVTDGERILGLGDLGCQ
Sbjct: 143 VGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERRIQVIVVTDGERILGLGDLGCQ 202

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKLSLY ALGGVRPSACLPI IDVGTNNEKLL +EFYIGLKQRRATGKEY E +
Sbjct: 203 GMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLANEFYIGLKQRRATGKEYSEFL 262

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
            EFM  VKQNYGEKVLIQFEDFANHNAF+LLAKY T+HL FNDDIQGTASVVLAG+VSAL
Sbjct: 263 QEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTHLAFNDDIQGTASVVLAGIVSAL 322

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           +L+GGTLADH FLFLGAGEAGTGIAELIA E+S+QT  PIEETRKKIWLVDSKGLIV SR
Sbjct: 323 RLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTKCPIEETRKKIWLVDSKGLIVGSR 382

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           KDSL  FKKPWAHEH+PVKDLL AVK IKPTVLIGSSGVG+ FTKEV+EAM+S N+KPLI
Sbjct: 383 KDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSSGVGKAFTKEVIEAMASCNEKPLI 442

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           +ALSNPTSQSECTAEEAYTW++GRAIFASGSPF+P +Y GKTFVPGQANNAYIFPG G+G
Sbjct: 443 LALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVEYNGKTFVPGQANNAYIFPGLGMG 502

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           ++ISGA+RVHD+MLLAA+EALA QV++EN+DKG+IYPPFS+IRKISAHIAANVAAKAYEL
Sbjct: 503 LVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYPPFSNIRKISAHIAANVAAKAYEL 562

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLATR P+P +LVKYAES MYSPVY
Sbjct: 563 GLATRLPQPENLVKYAESCMYSPVY 587

[Close]

BLAST of Carg12235 vs. ExPASy Swiss-Prot
Match: P34105 (NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3)

HSP 1 Score: 941.0 bits (2431), Expect = 6.2e-273
Identity = 468/587 (79.73%), Postives = 525/587 (89.44%), Query Frame = 0


Query: 1   MKSTLKE---------------VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPR 60
           M+STLKE               VG GV+D+ GE RATE QLVTP T SVASGY+LLRDP 
Sbjct: 1   MESTLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDPH 60

Query: 61  HNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNE 120
           HNKGLAFTEKERDAH+LRGLLPP   +QQLQEK+LM  IRQYQLPLQK+ AM+EL+ERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNE 120

Query: 121 RLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQK 180
           RLFYKLLI+NV ELLP+VYTPTVGEACQKYGSIF+RPQGLYISLKEKGK+++VLKNWPQK
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQK 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQG+GI VGKLSLY ALGGVRPSACLP+ IDVGTNNE+LL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLL 240

Query: 241 NDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
            DEFYIGL+QRRATG+EY EL+HEFM AVKQNYGEKVLIQFEDFANHNAF+LLAKY T+H
Sbjct: 241 KDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH 300

Query: 301 LVFNDDIQGTASVVLAGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNV 360
           LVFNDDIQGTA+VVLAG++SALKL+GG+LADHTFLFLGAGEAGTGIAELIA E+SR++  
Sbjct: 301 LVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKT 360

Query: 361 PIEETRKKIWLVDSKGLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWL DSKGLIV SRK+SL HFKKPWAHEH+PVK LL  VKAIKP VLIG+SG
Sbjct: 361 PLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTSG 420

Query: 421 VGRTFTKEVVEAMSSINQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKY 480
           VG+TFTKEV+EAM+S N+KPLI+ALSNPTSQSECTA+EAYTW+KG+AIFASGSPF+P +Y
Sbjct: 421 VGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVEY 480

Query: 481 KGKTFVPGQANNAYIFPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPP 540
           +GK FVPGQ+NNAYIFPG GLG++ISGA+RVHDDMLLAAAEALA Q+ +E   KG+IYPP
Sbjct: 481 EGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPP 540

Query: 541 FSSIRKISAHIAANVAAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
            S+IRKIS  IAANVAAKAYELGLATR PRP +LVK+AES MYSP Y
Sbjct: 541 LSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAY 587

[Close]

BLAST of Carg12235 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 3.7e-270
Identity = 460/564 (81.56%), Postives = 518/564 (91.84%), Query Frame = 0


Query: 9   GDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLPP 68
           G GV+D+ GE  ATE  L+TP T SVASG SLLRDPR+NKGLAFTE ERDAH+LRGLLPP
Sbjct: 22  GGGVRDLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTEGERDAHYLRGLLPP 81

Query: 69  AVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPTV 128
           +V  Q+LQEKRLM N+RQY++PL +++A+++LQERNERLFYKLLI+NV ELLP+VYTPTV
Sbjct: 82  SVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLFYKLLIDNVAELLPVVYTPTV 141

Query: 129 GEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQG 188
           GEACQKYGSIFRRPQGLYISLKEKGKI+EVLKNWP+KSIQVIVVTDGERILGLGDLGCQG
Sbjct: 142 GEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQG 201

Query: 189 MGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELVH 248
           MGI VGKLSLY ALGGVRPS+CLP+ IDVGTNNEKLLNDEFYIGL+QRRATG+EY   + 
Sbjct: 202 MGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRRATGQEYATFLD 261

Query: 249 EFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSALK 308
           EFM AVKQNYGEKVL+QFEDFANHNAF+LL KY +SHLVFNDDIQGTASVVLAG++++LK
Sbjct: 262 EFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVFNDDIQGTASVVLAGLLASLK 321

Query: 309 LIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSRK 368
           L+GGTLADHTFLFLGAGEAGTGIAELIA EVS+QT  P+EETRKKIWLVDSKGLIV SR 
Sbjct: 322 LVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKIWLVDSKGLIVSSRL 381

Query: 369 DSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLIM 428
           +SL  FKKPWAHEH+PVK LL AVKAIKPTVLIGSSG G+TFTKEVVE M+S+N+KPLI+
Sbjct: 382 ESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPLIL 441

Query: 429 ALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLGI 488
           ALSNPTSQSECTAEEAYTWSKGRAIFASGSPF+P +Y+GK FVPGQANNAYIFPGFGLG+
Sbjct: 442 ALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGLGL 501

Query: 489 LISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYELG 548
           ++SGA+RV D+MLLAA+EALAAQVS+ENYDKG+IYPPF++IRKISA+IAA VAAKAY+LG
Sbjct: 502 IMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAAKVAAKAYDLG 561

Query: 549 LATRRPRPADLVKYAESSMYSPVY 573
           LA+   RP DLVKYAES MYSP Y
Sbjct: 562 LASHLKRPKDLVKYAESCMYSPGY 585

[Close]

BLAST of Carg12235 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 7.0e-269
Identity = 452/565 (80.00%), Postives = 513/565 (90.80%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           VG GV+D+ GE  ATE   +TP + SVASGYSLLRDP HNKGLAFTEKERDAH+LRGLLP
Sbjct: 79  VGGGVEDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLP 138

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V    LQ K++M NIRQY++PLQ++ AM++LQERNERLFYKLLIEN+ ELLPIVYTPT
Sbjct: 139 PVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIVYTPT 198

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQKYG+IF+ PQGLYISLK+KGK++E+LKNWPQK IQVIVVTDGERILGLGDLGCQ
Sbjct: 199 VGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQ 258

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKLSLY ALGG+RPSACLPI IDVGTNNEK+LNDEFYIGL+QRRA+GKEY EL+
Sbjct: 259 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELM 318

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
           +EFM AVKQNYGEKVLIQFEDFANHNAF+LL KYRT+HLVFNDDIQGTASVVLAG++SAL
Sbjct: 319 NEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISAL 378

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           KL+GG+LADH FLFLGAGEAGTGIAELIA E+S+QTN P+EETRKKIWLVDSKGLIVRSR
Sbjct: 379 KLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLIVRSR 438

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
            DSL HFKKPWAH+H+PV   L AVKAIKPTVLIGSSG G+TFTKEVVEAMSS N+KP+I
Sbjct: 439 LDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPII 498

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           +ALSNPTSQSECTAE+AYTWS+GR IFASGSPF P +Y GK +V GQ+NNAYIFPGFGLG
Sbjct: 499 LALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPGFGLG 558

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           ++ISGA+RVHD+MLLAA+EALA QV++E++D G+IYPPF++IRKISAHIAA VAAKAYEL
Sbjct: 559 LIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYEL 618

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLA+R P+P +LV YAES MYSP Y
Sbjct: 619 GLASRLPQPENLVAYAESCMYSPKY 643

[Close]

BLAST of Carg12235 vs. ExPASy Swiss-Prot
Match: O82191 (NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 1.0e-267
Identity = 462/565 (81.77%), Postives = 513/565 (90.80%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           V  GV D+ GE  AT +  +TP + SV+SGYSLLRDPR+NKGLAFTEKERD H+LRGLLP
Sbjct: 13  VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V  Q+LQEKRL+ NIRQYQ PLQK++A+ ELQERNERLFYKLLI+NV ELLPIVYTPT
Sbjct: 73  PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQK+GSIFRRPQGL+ISLK+KGKI++VLKNWP+++IQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKL+LY+ALGGVRPSACLP+ IDVGTNNEKLLNDEFYIGL+Q+RATG+EY EL+
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELL 252

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
           +EFM AVKQNYGEKVLIQFEDFANHNAFELLAKY  +HLVFNDDIQGTASVVLAG+VSA 
Sbjct: 253 NEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQ 312

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           KL    LA+HTFLFLGAGEAGTGIAELIA  +S+Q N  +EE+RKKIWLVDSKGLIV SR
Sbjct: 313 KLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSR 372

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           KDSL  FKKPWAHEH+PVKDLL A+KAIKPTVLIGSSGVGR+FTKEV+EAMSSIN++PLI
Sbjct: 373 KDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLI 432

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           MALSNPT+QSECTAEEAYTWSKGRAIFASGSPF+P +Y+GK FV  QANNAYIFPGFGLG
Sbjct: 433 MALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLG 492

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           ++ISGA+RVHDDMLLAAAEALA QVSKENY+KGMIYP FSSIRKISA IAANVA KAYEL
Sbjct: 493 LVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYEL 552

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLA R PRP D+VK AESSMYSP Y
Sbjct: 553 GLAGRLPRPKDIVKCAESSMYSPTY 577

[Close]

BLAST of Carg12235 vs. ExPASy TrEMBL
Match: A0A6J1EWI2 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111436772 PE=3 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 567/572 (99.13%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKE GDGVQDING+HRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEEGDGVQDINGQHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL
Sbjct: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVS+QTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSKQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEV+EAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVIEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPF+PFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLATRRPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. ExPASy TrEMBL
Match: A0A6J1L339 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499410 PE=3 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 559/572 (97.73%), Postives = 569/572 (99.48%), Query Frame = 0


Query: 1   MKSTLKEVGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60
           MKSTLKE+GDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH
Sbjct: 1   MKSTLKEIGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAH 60

Query: 61  HLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120
           HLRGLLPPAVA+QQLQEK+LMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL
Sbjct: 61  HLRGLLPPAVASQQLQEKKLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELL 120

Query: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180
           PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG
Sbjct: 121 PIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILG 180

Query: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240
           LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG
Sbjct: 181 LGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATG 240

Query: 241 KEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVL 300
           KEYY+L+HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRT H VFNDDIQGTASVVL
Sbjct: 241 KEYYDLLHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTYHPVFNDDIQGTASVVL 300

Query: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSK 360
           AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVS+QTNVPIEETRKKIWLVDSK
Sbjct: 301 AGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSKQTNVPIEETRKKIWLVDSK 360

Query: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420
           GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS
Sbjct: 361 GLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSS 420

Query: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYI 480
           INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPF+PFKYKGKTFVPGQANNAYI
Sbjct: 421 INQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPFKYKGKTFVPGQANNAYI 480

Query: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANV 540
           FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKG+IYPPF++IRKISAHIAANV
Sbjct: 481 FPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGLIYPPFTNIRKISAHIAANV 540

Query: 541 AAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           AAKAYELGLAT RPRPADLVKYAESSMYSPVY
Sbjct: 541 AAKAYELGLATHRPRPADLVKYAESSMYSPVY 572

[Close]

BLAST of Carg12235 vs. ExPASy TrEMBL
Match: A0A6J1CV17 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111014522 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 513/587 (87.39%), Postives = 550/587 (93.70%), Query Frame = 0


Query: 1   MKSTLKEVGD---------------GVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPR 60
           M+STLKE+GD               GV+D+ GE  ATE+QL+TP T SVASGYSLLRDP 
Sbjct: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDVYGEDCATEEQLITPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNE 120
           HNKGLAFTEKERDAH+LRGLLPP++ TQQLQEK+LMQNIRQYQLPLQKFIAM+ELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQK 180
           RLFYKLLI+NV ELLP+VYTPTVGEACQKYGSIFRRPQGLYISLKEKGKI+EVLKNWPQ+
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGI VGKLSLY ALGGVRPSACLPI IDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATG+EYYEL+HEFM AVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300

Query: 301 LVFNDDIQGTASVVLAGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNV 360
           LVFNDDIQGTASVVLAG VSALKLIGGTLADHTFLFLGAGEAGTGIAELIA E+S++TNV
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKKTNV 360

Query: 361 PIEETRKKIWLVDSKGLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWLVDSKGLIV SRKDSL HFKKPWAHEH+PV+DLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVQDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAMSSINQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKY 480
           VGRTFTKEVVEA+SSIN+KPLIMALSNPTSQSECTAEEAY WS+GRAIFASGSPF+P +Y
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYNWSEGRAIFASGSPFDPVEY 480

Query: 481 KGKTFVPGQANNAYIFPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPP 540
            GKTFVPGQANNAYIFPGFGLG++ISGA+RVHDDMLLAA+EALAAQVS ENY+KG+IYPP
Sbjct: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSAENYEKGLIYPP 540

Query: 541 FSSIRKISAHIAANVAAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           F++IRKISAHIAANVAAKAYELGLATR PRPADL+KYAES MYSPVY
Sbjct: 541 FTNIRKISAHIAANVAAKAYELGLATRLPRPADLMKYAESCMYSPVY 587

[Close]

BLAST of Carg12235 vs. ExPASy TrEMBL
Match: A0A0A0LW91 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.3e-294
Identity = 515/587 (87.73%), Postives = 550/587 (93.70%), Query Frame = 0


Query: 1   MKSTLKEVGD---------------GVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPR 60
           M+STLKE+GD               GV+DI GE  ATE+QLVTP T SVASGYSLLRDP 
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNE 120
           HNKGLAFTEKERDAH+LRGLLPPA+ TQQLQEK+LMQNIRQYQLPLQKFIAM+ELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQK 180
           RLFYKLL++NV ELLP+VYTPTVGEACQKYGSIFRRPQGLYISLKEKGKI+EVLKNWPQ+
Sbjct: 121 RLFYKLLVDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGI VGKLSLY ALGGVRPSACLPI IDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATG+EYYEL+ EFM AVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNV 360
           LVFNDDIQGTA+VVLAG VSALKLIGGTLADHTFLFLGAGEAGTGIAELIA EVS+QT  
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PIEETRKKIWLVDSKGLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWLVDSKGLIV SRKDSL HFKKPWAHEH+PVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAMSSINQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKY 480
           VGRTFTKEVVEA+SSIN+KPLIMALSNPTSQSECTAEEAYTWS+GRAIFASGSPF+PF+Y
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 KGKTFVPGQANNAYIFPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPP 540
            GKTFVPGQ+NNAYIFPGFGLG++ISGA+RVHDDMLLAA+EALAAQVS+ENYDKG+IYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FSSIRKISAHIAANVAAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           F++IRKISA+IAANVAAKAYELGLATR PRPADLVKYAES MYSPVY
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVY 587

[Close]

BLAST of Carg12235 vs. ExPASy TrEMBL
Match: A0A5D3CG29 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 PE=3 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.6e-294
Identity = 513/587 (87.39%), Postives = 550/587 (93.70%), Query Frame = 0


Query: 1   MKSTLKEVGD---------------GVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPR 60
           M+STLKE+GD               GV+DI GE  ATE+QLVTP T SVASGYSLLRDP 
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNE 120
           HNKGLAFTEKERDAH+LRGLLPPA+ TQQLQEK+LMQNIRQYQLPLQKFIAM+ELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQK 180
           RLFYKLLI+NV ELLP+VYTPTVGEACQKYGSIFRRPQGLYISLKEKGKI+EVLKNWPQ+
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQGMGI VGKLSLY ALGGVRPSACLPI IDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQRRATG+EYYEL+ EFM AVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGVVSALKLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNV 360
           LVFNDDIQGTA+VVLAG VSALKLIGGTLADHTFLFLGAGEAGTGIAELIA E+S+QT  
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKA 360

Query: 361 PIEETRKKIWLVDSKGLIVRSRKDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWLVDSKGLIV SRKDSL HFK+PWAHEH+PVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKQPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAMSSINQKPLIMALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKY 480
           VGRTFTKEV+EA+SSIN+KPLIMALSNPTSQSECTAEEAYTWS+GRAIFASGSPF+PF+Y
Sbjct: 421 VGRTFTKEVIEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 KGKTFVPGQANNAYIFPGFGLGILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPP 540
            GKTFVPGQ+NNAYIFPGFGLG++ISGA+RVHDDMLLAA+EALAAQVS+ENYDKG+IYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FSSIRKISAHIAANVAAKAYELGLATRRPRPADLVKYAESSMYSPVY 573
           F++IRKISA+IAANVAAKAYELGLATR PRPADLVKYAES MYSPVY
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVY 587

[Close]

BLAST of Carg12235 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 923.7 bits (2386), Expect = 7.2e-269
Identity = 462/565 (81.77%), Postives = 513/565 (90.80%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           V  GV D+ GE  AT +  +TP + SV+SGYSLLRDPR+NKGLAFTEKERD H+LRGLLP
Sbjct: 13  VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V  Q+LQEKRL+ NIRQYQ PLQK++A+ ELQERNERLFYKLLI+NV ELLPIVYTPT
Sbjct: 73  PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQK+GSIFRRPQGL+ISLK+KGKI++VLKNWP+++IQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKL+LY+ALGGVRPSACLP+ IDVGTNNEKLLNDEFYIGL+Q+RATG+EY EL+
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELL 252

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
           +EFM AVKQNYGEKVLIQFEDFANHNAFELLAKY  +HLVFNDDIQGTASVVLAG+VSA 
Sbjct: 253 NEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQ 312

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           KL    LA+HTFLFLGAGEAGTGIAELIA  +S+Q N  +EE+RKKIWLVDSKGLIV SR
Sbjct: 313 KLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSR 372

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           KDSL  FKKPWAHEH+PVKDLL A+KAIKPTVLIGSSGVGR+FTKEV+EAMSSIN++PLI
Sbjct: 373 KDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLI 432

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           MALSNPT+QSECTAEEAYTWSKGRAIFASGSPF+P +Y+GK FV  QANNAYIFPGFGLG
Sbjct: 433 MALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLG 492

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           ++ISGA+RVHDDMLLAAAEALA QVSKENY+KGMIYP FSSIRKISA IAANVA KAYEL
Sbjct: 493 LVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYEL 552

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLA R PRP D+VK AESSMYSP Y
Sbjct: 553 GLAGRLPRPKDIVKCAESSMYSPTY 577

[Close]

BLAST of Carg12235 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 903.3 bits (2333), Expect = 1.0e-262
Identity = 439/565 (77.70%), Postives = 512/565 (90.62%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           VG G+ D+ GE  AT  QLVTP  +SVASGY+L+RDPR+NKGLAFT+KERDAH+L GLLP
Sbjct: 20  VGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLP 79

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P + +Q +QE+++M N+RQY +PLQ+++A+++LQERNERLFYKLLI+NV ELLP+VYTPT
Sbjct: 80  PVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPT 139

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQKYGSIFR+PQGLYISL EKGKI+EVLKNWPQ+ IQVIVVTDGERILGLGDLGCQ
Sbjct: 140 VGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQ 199

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKLSLY ALGG+RPSACLPI IDVGTNNEKLLNDEFYIGLKQRRATG+EY E +
Sbjct: 200 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFL 259

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
           HEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY  SHLVFNDDIQGTASVVLAG+++A 
Sbjct: 260 HEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQ 319

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           K++G  LADHTFLFLGAGEAGTGIAELIA ++S++T  PI ETRKKIWLVDSKGLIV SR
Sbjct: 320 KVLGKKLADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSR 379

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           K+SL HFK+PWAHEH+PVKDL+ AV AIKPTVLIG+SGVG+TFTKEVVEAM++ N+KPLI
Sbjct: 380 KESLQHFKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLI 439

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           +ALSNPTSQ+ECTAE+AYTW+KGRAIF SGSPF+P  Y GKT++PGQANN YIFPG GLG
Sbjct: 440 LALSNPTSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLG 499

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           +++SGA+RV DDMLLAA+EALAAQV++E+Y  G+IYPPFS+IR+ISA+IAA VAAK Y+L
Sbjct: 500 LIMSGAIRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDL 559

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLA+  PR  DLVK+AESSMYSPVY
Sbjct: 560 GLASNLPRAKDLVKFAESSMYSPVY 584

[Close]

BLAST of Carg12235 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 901.0 bits (2327), Expect = 5.0e-262
Identity = 434/565 (76.81%), Postives = 514/565 (90.97%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           V  GVQD+ GE  ATE   +TP + SVASGY+LLRDP HNKGLAF+ +ERDAH+LRGLLP
Sbjct: 78  VSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLP 137

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V +Q LQ K++M  +RQYQ+PLQK++AM++LQE NERLFYKLLI++V ELLP++YTPT
Sbjct: 138 PTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPT 197

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQKYGSIF RPQGL+ISLKEKGKI EVL+NWP+K+IQVIVVTDGERILGLGDLGCQ
Sbjct: 198 VGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQ 257

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKLSLY ALGGVRPSACLP+ IDVGTNNEKLLNDEFYIGL+QRRATG+EY EL+
Sbjct: 258 GMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELM 317

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
           HEFM AVKQNYGEKV+IQFEDFANHNAF+LLAKY T+HLVFNDDIQGTASVVLAG+++AL
Sbjct: 318 HEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAAL 377

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           + +GG+L+DH FLFLGAGEAGTGIAELIA E+S+++++P+EE RK IWLVDSKGLIV SR
Sbjct: 378 RFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSR 437

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           K+S+ HFKKPWAH+H+P+++L+ AVKAIKPTVLIG+SGVG+TFT++VVE M+ +N+KP+I
Sbjct: 438 KESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPII 497

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           ++LSNPTSQSECTAEEAYTWS+GRAIFASGSPF P +Y+GKTFVPGQANNAYIFPGFGLG
Sbjct: 498 LSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLG 557

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           +++SG +RVHDDMLLAA+EALA ++ +E+Y+KGMIYPPF +IRKISA IAA VAAKAYEL
Sbjct: 558 LIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYEL 617

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLATR P+P +L + AESSMYSP Y
Sbjct: 618 GLATRLPQPKELEQCAESSMYSPSY 642

[Close]

BLAST of Carg12235 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 899.0 bits (2322), Expect = 1.9e-261
Identity = 435/565 (76.99%), Postives = 514/565 (90.97%), Query Frame = 0


Query: 8   VGDGVQDINGEHRATEKQLVTPRTSSVASGYSLLRDPRHNKGLAFTEKERDAHHLRGLLP 67
           VG G+ D+ GE  AT  QLVTP  +SVASGY+L+RDPR+NKGLAFT+KERDAH++ GLLP
Sbjct: 20  VGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLP 79

Query: 68  PAVATQQLQEKRLMQNIRQYQLPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPT 127
           P V +Q +QE+++M N+RQY +PLQ+++A+++LQERNERLFYKLLI+NV ELLP+VYTPT
Sbjct: 80  PVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPT 139

Query: 128 VGEACQKYGSIFRRPQGLYISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQ 187
           VGEACQKYGSI+RRPQGLYISLKEKGKI+EVLKNWPQ+ IQVIVVTDGERILGLGDLGCQ
Sbjct: 140 VGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQ 199

Query: 188 GMGISVGKLSLYAALGGVRPSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELV 247
           GMGI VGKLSLY ALGG+RPSACLPI IDVGTNNEKLLN+EFYIGLKQ+RA G+EY E +
Sbjct: 200 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFL 259

Query: 248 HEFMMAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSAL 307
            EFM AVKQNYGEKVL+QFEDFANH+AFELL+KY +SHLVFNDDIQGTASVVLAG+++A 
Sbjct: 260 QEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQ 319

Query: 308 KLIGGTLADHTFLFLGAGEAGTGIAELIAYEVSRQTNVPIEETRKKIWLVDSKGLIVRSR 367
           K++G +LADHTFLFLGAGEAGTGIAELIA ++S++T  PI+ETRKKIWLVDSKGLIV  R
Sbjct: 320 KVLGKSLADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSER 379

Query: 368 KDSLPHFKKPWAHEHQPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAMSSINQKPLI 427
           K+SL HFK+PWAH+H+PVK+LL+AV AIKPTVLIG+SGVG+TFTKEVVEAM+++N+KPLI
Sbjct: 380 KESLQHFKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLI 439

Query: 428 MALSNPTSQSECTAEEAYTWSKGRAIFASGSPFEPFKYKGKTFVPGQANNAYIFPGFGLG 487
           +ALSNPTSQ+ECTAEEAYTW+KGRAIFASGSPF+P +Y GK F PGQANN YIFPG GLG
Sbjct: 440 LALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLG 499

Query: 488 ILISGAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYEL 547
           +++SGA+RV DDMLLAA+EALA+QV++EN+  G+IYPPF++IRKISA+IAA+V AK YEL
Sbjct: 500 LIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYEL 559

Query: 548 GLATRRPRPADLVKYAESSMYSPVY 573
           GLA+  PRP DLVK AES MYSPVY
Sbjct: 560 GLASNLPRPKDLVKMAESCMYSPVY 584

[Close]

BLAST of Carg12235 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 428.7 bits (1101), Expect = 7.3e-120
Identity = 245/571 (42.91%), Postives = 349/571 (61.12%), Query Frame = 0


Query: 37  GYSLLRDPRHNKGLAFTEKERDAHHLRGLLPPAVATQQLQEKRLMQNIRQYQ-------- 96
           G  +L DP  NKG AFT  ER+   LRGLLPP V   + Q  R M ++++ +        
Sbjct: 46  GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPS 105

Query: 97  --LPLQKFIAMIELQERNERLFYKLLIENVTELLPIVYTPTVGEACQKYGSIFRRPQGLY 156
               L K+  +  L +RNE ++YK+LI N+ E  PIVYTPTVG  CQ Y  +FRRP+G+Y
Sbjct: 106 DPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMY 165

Query: 157 ISLKEKGKIVEVLKNWPQKSIQVIVVTDGERILGLGDLGCQGMGISVGKLSLYAALGGVR 216
            S +++G+++ ++ NWP + + +IVVTDG RILGLGDLG  G+GI+VGKL LY A  G+ 
Sbjct: 166 FSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGIN 225

Query: 217 PSACLPIAIDVGTNNEKLLNDEFYIGLKQRRATGKEYYELVHEFMMAVKQNYGEKVLIQF 276
           P   LP+ IDVGTNNEKL ND  Y+GL+QRR    +Y +++ EFM AV   +   V++QF
Sbjct: 226 PQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-PHVIVQF 285

Query: 277 EDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGVVSALKLIGGTLADH---TFLFLG 336
           EDF +  AF+LL +YR ++ +FNDD+QGTA V +AG++ A++  G  + D      +  G
Sbjct: 286 EDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAG 345

Query: 337 AGEAGTGIAELIAYEVSR---QTNVPIEETRKKIWLVDSKGLIVRSRK----DSLPHFKK 396
           AG AG G+       ++R    T    +  + + W+VD++GLI   R+    ++ P  +K
Sbjct: 346 AGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQPFARK 405

Query: 397 PWAHEHQPVKD---LLSAVKAIKPTVLIGSSGVGRTFTKEVVEAM-SSINQKPLIMALSN 456
               E Q +K+   L+  V+ +KP VL+G S VG  F+KEV+EAM  S + +P I A+SN
Sbjct: 406 TKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSN 465

Query: 457 PTSQSECTAEEAYTWSKGRAIFASGSPFEPFKY-KGKTFVPGQANNAYIFPGFGLGILIS 516
           PT  +ECT ++A++      IFASGSPF+  ++  G      Q NN Y+FPG GLG L+S
Sbjct: 466 PTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLS 525

Query: 517 GAVRVHDDMLLAAAEALAAQVSKENYDKGMIYPPFSSIRKISAHIAANVAAKAYELGLA- 573
           GA  V D ML AA+E LAA +S+E   +G+IYPP S IR I+  IAA V  +A E  L  
Sbjct: 526 GAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVE 585

The following BLAST results are available for this feature:

BLAST of Carg12235 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021888.1	0.0e+00	100.00	hypothetical protein SDJN02_15616 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6587994.1	0.0e+00	99.30	hypothetical protein SDJN03_16559, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022930270.1	0.0e+00	99.13	NADP-dependent malic enzyme-like [Cucurbita moschata]	[more]
XP_023006774.1	0.0e+00	97.73	NADP-dependent malic enzyme-like [Cucurbita maxima]	[more]
XP_023531114.1	0.0e+00	97.55	NADP-dependent malic enzyme-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg12235 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51615	3.5e-276	83.54	NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
P34105	6.2e-273	79.73	NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3	[more]
P12628	3.7e-270	81.56	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
P36444	7.0e-269	80.00	NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ...	[more]
O82191	1.0e-267	81.77	NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 S...	[more]

BLAST of Carg12235 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWI2	0.0e+00	99.13	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111436772 PE=3 SV=1	[more]
A0A6J1L339	0.0e+00	97.73	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111499410 PE=3 SV=1	[more]
A0A6J1CV17	1.7e-294	87.39	Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111014522 PE=3 SV=1	[more]
A0A0A0LW91	2.3e-294	87.73	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1	[more]
A0A5D3CG29	8.6e-294	87.39	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 P...	[more]

BLAST of Carg12235 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19900.1	7.2e-269	81.77	NADP-malic enzyme 1	[more]
AT5G11670.1	1.0e-262	77.70	NADP-malic enzyme 2	[more]
AT1G79750.1	5.0e-262	76.81	NADP-malic enzyme 4	[more]
AT5G25880.1	1.9e-261	76.99	NADP-malic enzyme 3	[more]
AT2G13560.1	7.3e-120	42.91	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 107..131 score: 67.2 coord: 319..335 score: 71.32 coord: 263..281 score: 71.18 coord: 167..196 score: 80.5 coord: 203..225 score: 67.61 coord: 288..304 score: 75.44 coord: 422..438 score: 62.94
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 11..572 e-value: 1.3E-234 score: 778.0
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 292..545 e-value: 5.7E-113 score: 391.3
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 292..544 e-value: 8.3E-96 score: 320.2
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 101..282 e-value: 1.3E-100 score: 350.3
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 101..282 e-value: 6.8E-79 score: 263.9
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 35..289 e-value: 5.9E-124 score: 414.2
None	No IPR available	GENE3D	3.40.50.720		coord: 290..572 e-value: 8.8E-120 score: 401.6
None	No IPR available	PIRSR	PIRSR000106-3	PIRSR000106-3	coord: 39..372 e-value: 1.4E-140 score: 467.2
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 7..572
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 7..572
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 292..570 e-value: 3.13995E-144 score: 415.794
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 18..291
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 288..304
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 292..572

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12235-RA	Carg12235-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004470	malic enzyme activity