Carg12229 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12229
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Exostosin domain-containing protein
Location	Carg_Chr11: 2804478 .. 2805148 (+)
RNA-Seq Expression	Carg12229
Synteny	Carg12229

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACTCCGAGTCAGGAAAGTACCTAAGCGCTCCCTTTGGCATGCCAAGCCCTTGAAGTATGACATTACCCTTCATTCATTCTGGTATAACATAGTGTTTCAGATAAAGATATTGTAAGTGCTCCATTGAAGATATGAAGGAAATCCATGAAACTCTAAAATAACTGCACAGAACAAAATGACTCCTTTGCAGCTTCTCTTTGGAAAAAAAAATCTGCTTAAACCTCGGTGGGATCATGTCAAGGACCTTGAAAATGGACTGCAACTGTGGCAGTGTTCTTGTATATAGCAGACTGGTAAAACAAAACTATGATTATTTGTCAATATTGCAGTAGAAATCCTGATTTCCGTTCAGCAACCTCTGCATCTGGCTGTGAGACTTCAGGTTTCAAATCATACTGCCCTCTACCTCGTCGACGTAGGAAGCCATTTTCCAGGACCACAATGGAAGTTATTACTCGTTCTTTGGGTCATCATCAACCACATCCATCCAAAGCAATAAATGTTTTCACTCACTGGGTCCATCCAGACCAATTGAAGAGAGACCCACATTCAAGAACCGTATCTCTGAGATCGGAATCAGATACTCAGGCATATATGATCTCTAATATGGCACCACAACACGAAGCTTCCTATGAAGAACAGGGGCAATGTGGAATGGGCGATGAT

mRNA sequence

ATGCAACTCCGAGTCAGGAAAGTACCTAAGCGCTCCCTTTGGCATGCCAAGCCCTTGAAGTATGACATTACCCTTCATTCATTCTGGTATAACATAGTGTTTCAGATAAAGATATTTAGAAATCCTGATTTCCGTTCAGCAACCTCTGCATCTGGCTGTGAGACTTCAGGTTTCAAATCATACTGCCCTCTACCTCGTCGACGTAGGAAGCCATTTTCCAGGACCACAATGGAAGTTATTACTCGTTCTTTGGGTCATCATCAACCACATCCATCCAAAGCAATAAATGTTTTCACTCACTGGGTCCATCCAGACCAATTGAAGAGAGACCCACATTCAAGAACCGTATCTCTGAGATCGGAATCAGATACTCAGGCATATATGATCTCTAATATGGCACCACAACACGAAGCTTCCTATGAAGAACAGGGGCAATGTGGAATGGGCGATGAT

Coding sequence (CDS)

ATGCAACTCCGAGTCAGGAAAGTACCTAAGCGCTCCCTTTGGCATGCCAAGCCCTTGAAGTATGACATTACCCTTCATTCATTCTGGTATAACATAGTGTTTCAGATAAAGATATTTAGAAATCCTGATTTCCGTTCAGCAACCTCTGCATCTGGCTGTGAGACTTCAGGTTTCAAATCATACTGCCCTCTACCTCGTCGACGTAGGAAGCCATTTTCCAGGACCACAATGGAAGTTATTACTCGTTCTTTGGGTCATCATCAACCACATCCATCCAAAGCAATAAATGTTTTCACTCACTGGGTCCATCCAGACCAATTGAAGAGAGACCCACATTCAAGAACCGTATCTCTGAGATCGGAATCAGATACTCAGGCATATATGATCTCTAATATGGCACCACAACACGAAGCTTCCTATGAAGAACAGGGGCAATGTGGAATGGGCGATGAT

Protein sequence

MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKSYCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRSESDTQAYMISNMAPQHEASYEEQGQCGMGDD

Homology

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BLAST of Carg12229 vs. NCBI nr
Match: KAG6587988.1 (putative glycosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.5 bits (815), Expect = 3.0e-83
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS 60
           MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS
Sbjct: 171 MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS 230

Query: 61  YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS 120
           YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS
Sbjct: 231 YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS 290

Query: 121 ESDTQAYMISNMAPQHEASYEEQGQCGMGDD 152
           ESDTQAYMISNMAPQHEASYEEQGQCGMGDD
Sbjct: 291 ESDTQAYMISNMAPQHEASYEEQGQCGMGDD 321

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BLAST of Carg12229 vs. NCBI nr
Match: KAG7021882.1 (hypothetical protein SDJN02_15610, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.5 bits (815), Expect = 3.0e-83
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS 60
           MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS
Sbjct: 1   MQLRVRKVPKRSLWHAKPLKYDITLHSFWYNIVFQIKIFRNPDFRSATSASGCETSGFKS 60

Query: 61  YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS 120
           YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS
Sbjct: 61  YCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPHSRTVSLRS 120

Query: 121 ESDTQAYMISNMAPQHEASYEEQGQCGMGDD 152
           ESDTQAYMISNMAPQHEASYEEQGQCGMGDD
Sbjct: 121 ESDTQAYMISNMAPQHEASYEEQGQCGMGDD 151

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BLAST of Carg12229 vs. NCBI nr
Match: KAG6589943.1 (putative glycosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.8 bits (281), Expect = 2.5e-21
Identity = 72/152 (47.37%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKIFRNPDFRSATSASGCETSG 60
           MQLRVRKV K  LWHAKPLKYD+   TLHS W                            
Sbjct: 608 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIW---------------------------- 667

Query: 61  FKSYCPLPRRRRKPFSRTTMEVITRSLGHHQPHPSKAINVFTHWVHPDQLKRDPH-SRTV 120
                            TTME IT S GHH PHPSKAINVFTHWVHP     +PH  +  
Sbjct: 668 -----------------TTMEGITLSFGHH-PHPSKAINVFTHWVHPVLQLEEPHLQKPY 713

Query: 121 SLRSESDTQAYMISNMAPQHEASYEEQGQCGM 149
           S  SESD+ A     MAPQHEASYE+Q QC M
Sbjct: 728 SQGSESDSYAIYHIYMAPQHEASYEKQRQCAM 713

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BLAST of Carg12229 vs. NCBI nr
Match: XP_038879145.1 (probable glycosyltransferase At5g03795 [Benincasa hispida] >XP_038879147.1 probable glycosyltransferase At5g03795 [Benincasa hispida] >XP_038879148.1 probable glycosyltransferase At5g03795 [Benincasa hispida])

HSP 1 Score: 64.3 bits (155), Expect = 1.0e-06
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 635 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 675

[Close]

BLAST of Carg12229 vs. NCBI nr
Match: XP_023516188.1 (probable glycosyltransferase At5g03795 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 64.3 bits (155), Expect = 1.0e-06
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 629 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 669

[Close]

BLAST of Carg12229 vs. ExPASy TrEMBL
Match: A0A6J1JEU9 (probable glycosyltransferase At5g03795 OS=Cucurbita maxima OX=3661 GN=LOC111485134 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.0e-07
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 634 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 674

[Close]

BLAST of Carg12229 vs. ExPASy TrEMBL
Match: A0A0A0LU64 (Exostosin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G364960 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.0e-07
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 623 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 663

[Close]

BLAST of Carg12229 vs. ExPASy TrEMBL
Match: A0A6J1H980 (probable glycosyltransferase At5g03795 OS=Cucurbita moschata OX=3662 GN=LOC111461655 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.0e-07
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 629 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 669

[Close]

BLAST of Carg12229 vs. ExPASy TrEMBL
Match: A0A1S3CJA3 (probable glycosyltransferase At5g03795 OS=Cucumis melo OX=3656 GN=LOC103501046 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.0e-07
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV K  LWHAKPLKYD+   TLHS WYN VFQIK+
Sbjct: 623 MQLRVRKVQKHFLWHAKPLKYDLFHMTLHSIWYNRVFQIKL 663

[Close]

BLAST of Carg12229 vs. ExPASy TrEMBL
Match: A0A059CN85 (Exostosin domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C01012 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.5e-06
Identity = 29/41 (70.73%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIKI 39
           MQLRVRKV +  LWHAKP+KYD+   TLHS WYN VFQIK+
Sbjct: 655 MQLRVRKVQRHFLWHAKPVKYDLFYMTLHSIWYNRVFQIKL 695

[Close]

BLAST of Carg12229 vs. TAIR 10
Match: AT5G25820.1 (Exostosin family protein )

HSP 1 Score: 55.5 bits (132), Expect = 4.4e-08
Identity = 26/41 (63.41%), Postives = 30/41 (73.17%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYD---ITLHSFWYNIVFQIKI 39
           MQ+RV+KV K  LWHAKP KYD   + LHS WYN VFQI +
Sbjct: 614 MQMRVKKVQKHFLWHAKPEKYDMFHMILHSIWYNRVFQISV 654

[Close]

BLAST of Carg12229 vs. TAIR 10
Match: AT5G19670.1 (Exostosin family protein )

HSP 1 Score: 51.2 bits (121), Expect = 8.4e-07
Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQIK 38
           MQ+ VRK  +  LWHAKP KYD+    LHS WYN VFQ K
Sbjct: 569 MQMAVRKAQRHFLWHAKPEKYDLFHMVLHSIWYNRVFQAK 608

[Close]

BLAST of Carg12229 vs. TAIR 10
Match: AT4G32790.1 (Exostosin family protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.2e-06
Identity = 23/39 (58.97%), Postives = 28/39 (71.79%), Query Frame = 0


Query: 1   MQLRVRKVPKRSLWHAKPLKYDI---TLHSFWYNIVFQI 37
           MQ+RV+ V K  LWH+KP ++DI    LHS WYN VFQI
Sbjct: 555 MQMRVKMVQKHFLWHSKPERFDIFHMILHSIWYNRVFQI 593

The following BLAST results are available for this feature:

BLAST of Carg12229 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587988.1	3.0e-83	100.00	putative glycosyltransferase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7021882.1	3.0e-83	100.00	hypothetical protein SDJN02_15610, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6589943.1	2.5e-21	47.37	putative glycosyltransferase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038879145.1	1.0e-06	75.61	probable glycosyltransferase At5g03795 [Benincasa hispida] >XP_038879147.1 proba...	[more]
XP_023516188.1	1.0e-06	75.61	probable glycosyltransferase At5g03795 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg12229 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg12229 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JEU9	5.0e-07	75.61	probable glycosyltransferase At5g03795 OS=Cucurbita maxima OX=3661 GN=LOC1114851...	[more]
A0A0A0LU64	5.0e-07	75.61	Exostosin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G364960 P...	[more]
A0A6J1H980	5.0e-07	75.61	probable glycosyltransferase At5g03795 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A1S3CJA3	5.0e-07	75.61	probable glycosyltransferase At5g03795 OS=Cucumis melo OX=3656 GN=LOC103501046 P...	[more]
A0A059CN85	2.5e-06	70.73	Exostosin domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C0...	[more]

BLAST of Carg12229 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25820.1	4.4e-08	63.41	Exostosin family protein	[more]
AT5G19670.1	8.4e-07	60.00	Exostosin family protein	[more]
AT4G32790.1	3.2e-06	58.97	Exostosin family protein	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12229-RA	Carg12229-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006486	protein glycosylation
cellular_component	GO:0000139	Golgi membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016757	glycosyltransferase activity