Carg12216 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12216
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAuxin-responsive protein
LocationCarg_Chr11: 2754585 .. 2758064 (+)
RNA-Seq ExpressionCarg12216
SyntenyCarg12216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACCCTAACCCTAACCCTAATAAACCTAAACCCTAACCGTGGGGATTCAATTTCTTTTTTGGCCTGAGAAATTCAGAATCAGAAGGGGGAGGTGTAAATTCCCTCACTTTCCACCATTTCTCTTTCTGTTTCTTAGGTTTTTTGTTTTTAAAAACAACATTTAGGGCAACGGACTTCAAAATCTGGCGAGTTCCTACAGACTGACTCTGCCCCCGACCTTTCCCTTTCCGCATTACCGTGTTACGCTGCTTTCAATTTTCACTCTACTGTTTTGATGCTGTCGCTCTGCGCAGGTTAGCGTTGTTTTTCTAAAAATTATATAGAAAGAAAGAAAGAAAGAAGGGCAATTGCTTGCTTGCTTAATTAAATATTAATTAGGGAAAGGTGGAGGGTTTAGGGTTTGTGTGACTTGTCGGGGTGGCTGCCCACAAAAAGGTAGATTGAATTATAAAATCAAAACAAATCCTGAAAAGAAGCAGCACACAAATTTTTATTTAATTTATTTAAACTAAATCAAGTTCCATCCCCCCACTCCTCTCTGCTCCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTATTTAATAATTCCCTCTTTTCTTTTTCTTTCCATTTCTCCTCTTCCTCACACTCGCACTACCTTTCACACTTCAAACAACGACACAAACCAACGGTGTTCAACAATCTTCAACAACCTTCTCTTCTTAAACTCCATTTGATTTTCTCTTCTTCTTCTGTTTTTTCCCATGCTTTTTGTGTATTTATCTTGAGCTTTCAAGTGTTTCTGATGTCTATACCCTTAGAACATGATTACATAGGTTTGACAGACTCTGTTCCTTCCCTGGAAACCTCTGAAATGTCCTCCGACACACGCAACAATCCTGGGTTGAACCTTAAGGCCACTGAGCTCAGGCTGGGCCTGCCTGGGTCCGACTCACCGCTCAGGGATGATGGCCTCGACGACACTAATGGATACCCTCACAAGACCTCTGTTTCCGGGGCCAAGAGGGGTTTCTCTGTAGCCATTGATAGAGCTTCTTCCAAGTGGGTTCTGCCTGCCTCTGCCGGATCTGAACCTGATTCCACCAAAACTGGGGGCTTGTTTTCTCCCAGAGTGGTTACTGAGAATAAGACTCAACCACCTGCTTCGCCTGTTTCTGGTGCTAAAGATGGTATTTCTTCGTTGCCAAAGCCACTGCTCGAGGAAAAGCCGCAGCTTTCTCCCCCTGCAGCCAAGTATGCTTTCTTCCATTTCTTTTTCCCTCCCCGCTAGACTCTGCTTTGGAACTCCCCCATGTTCAACATTAGTTTCTTTCCCTGCATTCCCTTTAACGTTCTTTCTAGCTTCTTCTCCGATTTAAATGAGAATTTTGTGTTGGGATTCAATCATGTAGCTGTAGTCCTTACTTTCTCTATGACCCTTCATCCACCATGTTTAGATTAGGAATTAATTTGAACAGATTTCTGTGGCCGTTTCATTAGAATTTTAATGTTTTTACATGCAGATCTCCTTTTAAATTTCCCCACCTGGAAAACAGTTATATATGTTGGATTACAAGATACTAAATGTTCTTTATCATGCCTTTACAGGGCACAAGTTGTAGGATGGCCACCCATTCGTTCTTTCCGGAAGAATTCAATGGCAACGCAGCCCCCTAAAAATACAGATGATGCAGACGGTAAGATGGGATCGGGTTGCCTTTATGTGAAGGTAAGTATGGATGGCGCACCATACCTCAGGAAGGTTGATCTGAAAACCTATGGAAGCTATTTGGACCTCTCATCAGCCCTTGGGAAAATGTTCAGCAGCTTCACAATTGGTATGTTCTTTATGGCCACTCCTACACATCTTGCCTGATTTAGTAATCTTAATTACACCTTCCATAGTTTATTGGCAAGCACTTCCAGAATTGCGGATGTTTTAGATTTGACATGTATGGAACGTGTTCGTATTTGTTCTAAATTGTTGCTGAATCACCACCCTTTACATGTCTAATTCTTTCCTCTAACTAAAACCAAAACCTACTAGAAGGTCAAGAACAGAACACCTGGTGCACGTGAACTAGTTTATGTTGCTCAGTTTCTCTCATGTGTAGATTTTGTAGCTAGTTTTTGTTTGTTCAATTATTAGTGACTTTCTCTTTGCTACTGCTCCATTCATTAAATCAAGGTCATTGTGGCTCCAATGGAATTCCAAATCGGGATGCATTAAACGAGAGTCGATTGATGGATCTGCTCCATGGCTCTGAATATGTACTCACCTATGAAGATAAGGATGGCGACTGGATGCTCGTTGGTGATGTTCCATGGGAGTAAGTTCATTAAAATAACTCTATTGCAGTGGCAGTTCTTAACTGACTTCTTAGTGTTTCAATCTCTAAATGACTGGCTGCTAAAACAAGTCTACATCTTGTTCGTCTTTTTCTTGTTACATAACAGGATGTTCACTGAATCTTGCACGAGGATGAGGATCATGAAGAGCTCAGAGGCTATTGGGTTAGGTATGCTATGCTATAGACTTAAATAATTTCTTAATTTTGTGTTTCTTACAAAATGAAATCAGACAAGTTATAGTGGGATTTCATCTTCTTCATGATTATAAGTTTTGAGAATTTGGGCCTTTCTTCAGTGCTCTCTTAGTTACCATCATTATCTCTCCACTTCTAAAGCCTTTTCTTTTTCTTAGAATTAAGCATCTTTCATTTTCATAAAATTTGATCCCCACCCCTAAAGAAAAAGATCTATCAAACAAAACATATTAGTTTAAATTCCATTATGATCGGATCTTTTAACAAAGTTTATCATAATCTGATGCTTTAGTCATTGCCATTACAAGGAGATGCTATTTCGTGATATGGATTGAGCAATATCTAATAAAAATGGGGAATCAGAATAAAAAGTTAACCCCTTCCAAAAAAAGAAAAGAAAAAAAAAAAAAAAAAAACTTCTCCAGTGACCCCATTTGTAAAAGCTTGGCCTTTCTCTTTTTCTTTTTCTTACAGCTCCCAGGGCCATGGAGAAGCGCAGAAACCTTAACTAGCAAACTCATTTGCTATCGATCGGGCACGTTCGAGCCAATGATGCTCAACGATTGACGTAGTAACATGTGTGCTGGATGTAGATCGAGCGAGAGAATCTTTACTGAATATTGTTGTTCTTTGTGTATGAGTTCTAAATATTGGAGAGTTGATGTATGAAGATAAGTTCTTTGTTAGCAAAGATGATTTTCAATAGGACGATTCAGAGTTTCTTAGTTTGCCCCTCCCCCTCTTCTTTGCCATTTTACTTCGTTTGCTTTACTACACTTTACTTCGTATTGTAAATGATTTGTTTGCTATGGCAATGGTATCCTCACCCATCATAAATTGTGTTCCTCTTTCTGGATAGAGTATTCAAATCAGCTATCACCAAGATTTTAACAGTGTTCTGTTCTTCACATAATAGGAAATTGATGAATATGATCATTCATATCAATTA

mRNA sequence

TAAACCCTAACCCTAACCCTAATAAACCTAAACCCTAACCGTGGGGATTCAATTTCTTTTTTGGCCTGAGAAATTCAGAATCAGAAGGGGGAGGTGTAAATTCCCTCACTTTCCACCATTTCTCTTTCTGTTTCTTAGGTTTTTTGTTTTTAAAAACAACATTTAGGGCAACGGACTTCAAAATCTGGCGAGTTCCTACAGACTGACTCTGCCCCCGACCTTTCCCTTTCCGCATTACCGTGTTACGCTGCTTTCAATTTTCACTCTACTGTTTTGATGCTGTCGCTCTGCGCAGTGTTTCTGATGTCTATACCCTTAGAACATGATTACATAGGTTTGACAGACTCTGTTCCTTCCCTGGAAACCTCTGAAATGTCCTCCGACACACGCAACAATCCTGGGTTGAACCTTAAGGCCACTGAGCTCAGGCTGGGCCTGCCTGGGTCCGACTCACCGCTCAGGGATGATGGCCTCGACGACACTAATGGATACCCTCACAAGACCTCTGTTTCCGGGGCCAAGAGGGGTTTCTCTGTAGCCATTGATAGAGCTTCTTCCAAGTGGGTTCTGCCTGCCTCTGCCGGATCTGAACCTGATTCCACCAAAACTGGGGGCTTGTTTTCTCCCAGAGTGGTTACTGAGAATAAGACTCAACCACCTGCTTCGCCTGTTTCTGGTGCTAAAGATGGTATTTCTTCGTTGCCAAAGCCACTGCTCGAGGAAAAGCCGCAGCTTTCTCCCCCTGCAGCCAAGGCACAAGTTGTAGGATGGCCACCCATTCGTTCTTTCCGGAAGAATTCAATGGCAACGCAGCCCCCTAAAAATACAGATGATGCAGACGGTAAGATGGGATCGGGTTGCCTTTATGTGAAGGTAAGTATGGATGGCGCACCATACCTCAGGAAGGTTGATCTGAAAACCTATGGAAGCTATTTGGACCTCTCATCAGCCCTTGGGAAAATGTTCAGCAGCTTCACAATTGGTCATTGTGGCTCCAATGGAATTCCAAATCGGGATGCATTAAACGAGAGTCGATTGATGGATCTGCTCCATGGCTCTGAATATGTACTCACCTATGAAGATAAGGATGGCGACTGGATGCTCGTTGGTGATGTTCCATGGGAGATGTTCACTGAATCTTGCACGAGGATGAGGATCATGAAGAGCTCAGAGGCTATTGGGTTAGCTCCCAGGGCCATGGAGAAGCGCAGAAACCTTAACTAGCAAACTCATTTGCTATCGATCGGGCACGTTCGAGCCAATGATGCTCAACGATTGACGTAGTAACATGTGTGCTGGATGTAGATCGAGCGAGAGAATCTTTACTGAATATTGTTGTTCTTTGTGTATGAGTTCTAAATATTGGAGAGTTGATGTATGAAGATAAGTTCTTTGTTAGCAAAGATGATTTTCAATAGGACGATTCAGAGTTTCTTAGTTTGCCCCTCCCCCTCTTCTTTGCCATTTTACTTCGTTTGCTTTACTACACTTTACTTCGTATTGTAAATGATTTGTTTGCTATGGCAATGGTATCCTCACCCATCATAAATTGTGTTCCTCTTTCTGGATAGAGTATTCAAATCAGCTATCACCAAGATTTTAACAGTGTTCTGTTCTTCACATAATAGGAAATTGATGAATATGATCATTCATATCAATTA

Coding sequence (CDS)

ATGCTGTCGCTCTGCGCAGTGTTTCTGATGTCTATACCCTTAGAACATGATTACATAGGTTTGACAGACTCTGTTCCTTCCCTGGAAACCTCTGAAATGTCCTCCGACACACGCAACAATCCTGGGTTGAACCTTAAGGCCACTGAGCTCAGGCTGGGCCTGCCTGGGTCCGACTCACCGCTCAGGGATGATGGCCTCGACGACACTAATGGATACCCTCACAAGACCTCTGTTTCCGGGGCCAAGAGGGGTTTCTCTGTAGCCATTGATAGAGCTTCTTCCAAGTGGGTTCTGCCTGCCTCTGCCGGATCTGAACCTGATTCCACCAAAACTGGGGGCTTGTTTTCTCCCAGAGTGGTTACTGAGAATAAGACTCAACCACCTGCTTCGCCTGTTTCTGGTGCTAAAGATGGTATTTCTTCGTTGCCAAAGCCACTGCTCGAGGAAAAGCCGCAGCTTTCTCCCCCTGCAGCCAAGGCACAAGTTGTAGGATGGCCACCCATTCGTTCTTTCCGGAAGAATTCAATGGCAACGCAGCCCCCTAAAAATACAGATGATGCAGACGGTAAGATGGGATCGGGTTGCCTTTATGTGAAGGTAAGTATGGATGGCGCACCATACCTCAGGAAGGTTGATCTGAAAACCTATGGAAGCTATTTGGACCTCTCATCAGCCCTTGGGAAAATGTTCAGCAGCTTCACAATTGGTCATTGTGGCTCCAATGGAATTCCAAATCGGGATGCATTAAACGAGAGTCGATTGATGGATCTGCTCCATGGCTCTGAATATGTACTCACCTATGAAGATAAGGATGGCGACTGGATGCTCGTTGGTGATGTTCCATGGGAGATGTTCACTGAATCTTGCACGAGGATGAGGATCATGAAGAGCTCAGAGGCTATTGGGTTAGCTCCCAGGGCCATGGAGAAGCGCAGAAACCTTAACTAG

Protein sequence

MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAMEKRRNLN
Homology
BLAST of Carg12216 vs. NCBI nr
Match: KAG7021869.1 (Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60
           MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP
Sbjct: 1   MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60

Query: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120
           LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV
Sbjct: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120

Query: 121 TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180
           TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP
Sbjct: 121 TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180

Query: 181 PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240
           PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS
Sbjct: 181 PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240

Query: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300
           NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA
Sbjct: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300

Query: 301 IGLAPRAMEKRRNLN 316
           IGLAPRAMEKRRNLN
Sbjct: 301 IGLAPRAMEKRRNLN 315

BLAST of Carg12216 vs. NCBI nr
Match: XP_023531223.1 (auxin-responsive protein IAA27-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.2 bits (1580), Expect = 1.2e-171
Identity = 303/306 (99.02%), Postives = 306/306 (100.00%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT
Sbjct: 1   MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA
Sbjct: 61  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 189
           SPVSGAKDG+S+LPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKN+DDADG
Sbjct: 121 SPVSGAKDGMSTLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNSDDADG 180

Query: 190 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 249
           KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL
Sbjct: 181 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 240

Query: 250 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 309
           NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME
Sbjct: 241 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 300

Query: 310 KRRNLN 316
           KRRNLN
Sbjct: 301 KRRNLN 306

BLAST of Carg12216 vs. NCBI nr
Match: KAG6587978.1 (Auxin-responsive protein IAA27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.1 bits (1577), Expect = 2.7e-171
Identity = 307/315 (97.46%), Postives = 307/315 (97.46%), Query Frame = 0

Query: 1   MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60
           MLSLCA        EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP
Sbjct: 1   MLSLCA--------EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60

Query: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120
           LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV
Sbjct: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120

Query: 121 TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180
           TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP
Sbjct: 121 TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180

Query: 181 PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240
           PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS
Sbjct: 181 PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240

Query: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300
           NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA
Sbjct: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300

Query: 301 IGLAPRAMEKRRNLN 316
           IGLAPRAMEKRRNLN
Sbjct: 301 IGLAPRAMEKRRNLN 307

BLAST of Carg12216 vs. NCBI nr
Match: XP_022933814.1 (auxin-responsive protein IAA27-like [Cucurbita moschata])

HSP 1 Score: 610.9 bits (1574), Expect = 6.1e-171
Identity = 303/306 (99.02%), Postives = 304/306 (99.35%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT
Sbjct: 1   MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSP VVTEN TQPPA
Sbjct: 61  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPGVVTENMTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 189
           SPVSGAKDGIS+LPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG
Sbjct: 121 SPVSGAKDGISTLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 180

Query: 190 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 249
           KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL
Sbjct: 181 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 240

Query: 250 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 309
           NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME
Sbjct: 241 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 300

Query: 310 KRRNLN 316
           KRRNLN
Sbjct: 301 KRRNLN 306

BLAST of Carg12216 vs. NCBI nr
Match: XP_023531224.1 (auxin-responsive protein IAA27-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.3 bits (1531), Expect = 5.9e-166
Identity = 299/315 (94.92%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MLSLCAVFLMSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60
           MLSLCA             GLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP
Sbjct: 1   MLSLCA-------------GLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSP 60

Query: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120
           LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV
Sbjct: 61  LRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVV 120

Query: 121 TENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180
           TENKTQPPASPVSGAKDG+S+LPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP
Sbjct: 121 TENKTQPPASPVSGAKDGMSTLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQP 180

Query: 181 PKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240
           PKN+DDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS
Sbjct: 181 PKNSDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 240

Query: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300
           NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA
Sbjct: 241 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 300

Query: 301 IGLAPRAMEKRRNLN 316
           IGLAPRAMEKRRNLN
Sbjct: 301 IGLAPRAMEKRRNLN 302

BLAST of Carg12216 vs. ExPASy Swiss-Prot
Match: Q9ZSY8 (Auxin-responsive protein IAA27 OS=Arabidopsis thaliana OX=3702 GN=IAA27 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.4e-80
Identity = 186/321 (57.94%), Postives = 219/321 (68.22%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTR---NNPGLNLKATELRLGLPGSDSPLRDDGL 69
           +S+  EHDYIGL++  P++E + MS  T+   NN GLN KATELRLGLPGS+SP R   +
Sbjct: 3   VSVAAEHDYIGLSE-FPTMEATTMSDKTKTRDNNNGLNFKATELRLGLPGSESPER---V 62

Query: 70  DDTNGYPHKTS--VSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPR--VVTE 129
           D      +K+S  VSGAKR FS AI+  S+KWV   S GS   +   G    PR  VV +
Sbjct: 63  DSRFLALNKSSCPVSGAKRVFSDAIN-DSNKWVF--SPGSTTATGDVGSGSGPRTSVVKD 122

Query: 130 NKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMA---TQ 189
            K+     P             P+ E+K   + PA+KAQVVGWPPIRSFRKNSMA   +Q
Sbjct: 123 GKSTTFTKPA-----------VPVKEKKSSATAPASKAQVVGWPPIRSFRKNSMASSQSQ 182

Query: 190 PPKN---TDDADGKMG--SGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFT 249
            P N   T++A+ K G    CLYVKVSM+GAPYLRK+DLKTY SYL+LSSAL KMFS FT
Sbjct: 183 KPGNNSETEEAEAKSGPEQPCLYVKVSMEGAPYLRKIDLKTYKSYLELSSALEKMFSCFT 242

Query: 250 IGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRI 309
           IG  GS+G   RD LNESRL DLL GSEYV+TYEDKD DWMLVGDVPWEMF  SC ++RI
Sbjct: 243 IGQFGSHGGCGRDGLNESRLTDLLRGSEYVVTYEDKDSDWMLVGDVPWEMFICSCKKLRI 302

Query: 310 MKSSEAIGLAPRAMEKRRNLN 316
           MKSSEAIGLAPR MEK R+ N
Sbjct: 303 MKSSEAIGLAPRVMEKCRSRN 305

BLAST of Carg12216 vs. ExPASy Swiss-Prot
Match: Q38826 (Auxin-responsive protein IAA8 OS=Arabidopsis thaliana OX=3702 GN=IAA8 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-70
Identity = 161/320 (50.31%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 15  EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLR--DDGL------ 74
           E +Y+GL+D   S   S    +      LN KATELRLGLP S SP R  D GL      
Sbjct: 21  ERNYLGLSDC--SSVDSSTIPNVVGKSNLNFKATELRLGLPESQSPERETDFGLLSPRTP 80

Query: 75  DDTNGYP-------------HKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGG 134
           D+   +P             HK  VSG KRGF+   D  S         GS         
Sbjct: 81  DEKLLFPLLPSKDNGSATTGHKNVVSGNKRGFADTWDEFS------GVKGSVRPGGGINM 140

Query: 135 LFSPRVVTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRK 194
           + SP+V   +K+       S AK G++             + PAAKAQVVGWPPIRS+RK
Sbjct: 141 MLSPKVKDVSKSIQEER--SHAKGGLN-------------NAPAAKAQVVGWPPIRSYRK 200

Query: 195 NSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSF 254
           N+MA+   KNTD+ DGK G G L+VKVSMDGAPYLRKVDL+TY SY  LSSAL KMFS F
Sbjct: 201 NTMASSTSKNTDEVDGKPGLGVLFVKVSMDGAPYLRKVDLRTYTSYQQLSSALEKMFSCF 260

Query: 255 TIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMR 314
           T+G CG +G   R+ ++E +L DLLHGSE+VLTYEDKDGDWMLVGDVPWE+FTE+C +++
Sbjct: 261 TLGQCGLHGAQGRERMSEIKLKDLLHGSEFVLTYEDKDGDWMLVGDVPWEIFTETCQKLK 317

BLAST of Carg12216 vs. ExPASy Swiss-Prot
Match: Q38827 (Auxin-responsive protein IAA9 OS=Arabidopsis thaliana OX=3702 GN=IAA9 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.7e-70
Identity = 155/323 (47.99%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 15  EHDYIGLTD--SVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGL------ 74
           EH+Y+GL+D  SV S   S ++ D +    ++LKATEL LGLPGS SP RD  L      
Sbjct: 33  EHNYLGLSDCSSVGSSTLSPLAEDDKAT--ISLKATELTLGLPGSQSPARDTELNLLSPA 92

Query: 75  --------------DDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTG 134
                         D+      K + SG KRGFS  +D+ +               T+  
Sbjct: 93  KLDEKPFFPLLPSKDEICSSSQKNNASGNKRGFSDTMDQFAE--------AKSSVYTEKN 152

Query: 135 GLFSPRVVTENKTQPPASPVSGAKDGISSLPK-PLLEEKPQLSPPAAKAQVVGWPPIRSF 194
            +F     T++ T+         KD   ++PK          SPPAAKAQ+VGWPP+RS+
Sbjct: 153 WMFPEAAATQSVTK---------KDVPQNIPKGQSSTTNNSSSPPAAKAQIVGWPPVRSY 212

Query: 195 RKNSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFS 254
           RKN++AT   KN+D+ DG+ GSG L+VKVSMDGAPYLRKVDL++Y +Y +LSSAL KMF+
Sbjct: 213 RKNTLAT-TCKNSDEVDGRPGSGALFVKVSMDGAPYLRKVDLRSYTNYGELSSALEKMFT 272

Query: 255 SFTIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTR 313
           +FT+G CGSNG   +D L+E++L DLL+G +YVLTYEDKDGDWMLVGDVPWEMF + C +
Sbjct: 273 TFTLGQCGSNGAAGKDMLSETKLKDLLNGKDYVLTYEDKDGDWMLVGDVPWEMFIDVCKK 332

BLAST of Carg12216 vs. ExPASy Swiss-Prot
Match: P0C132 (Auxin-responsive protein IAA30 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA30 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-64
Identity = 148/284 (52.11%), Postives = 174/284 (61.27%), Query Frame = 0

Query: 43  LNLKATELRLGLPGSDSPLRDDGLDDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASA 102
           L  +ATELRLGLPG        G  D +        S  KRGF+  ID      + PA+A
Sbjct: 5   LAFEATELRLGLPG--------GGGDGDAAAAAARSSSGKRGFAETIDLKLK--LEPAAA 64

Query: 103 GSEPDSTKTGGLFSPRV-------VTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSP 162
             + D  K       R           +   PPA+  +G      S    +        P
Sbjct: 65  AVDDDDDKEEAAADDREKKVDIVGADNDDASPPAAAAAGGMKRSPSQSSVVTAAADPEKP 124

Query: 163 PAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG---KMGSG---CLYVKVSMDGAPYLR 222
            A KAQVVGWPP+RS+RKN +A Q  K  D ADG   K G+G     +VKVSMDGAPYLR
Sbjct: 125 RAPKAQVVGWPPVRSYRKNILAVQADKGKDAADGGGDKSGAGAAAAAFVKVSMDGAPYLR 184

Query: 223 KVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYED 282
           KVDLK Y SYL+LS AL KMFSSFTIG+CGS+G+   + +NES++ DLL+GSEYV TYED
Sbjct: 185 KVDLKMYKSYLELSKALEKMFSSFTIGNCGSHGV---NGMNESKIADLLNGSEYVPTYED 244

Query: 283 KDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAMEKRRN 314
           KDGDWMLVGDVPWEMF ESC R+RIMK SEAIGLAPRAMEK +N
Sbjct: 245 KDGDWMLVGDVPWEMFVESCKRLRIMKGSEAIGLAPRAMEKCKN 275

BLAST of Carg12216 vs. ExPASy Swiss-Prot
Match: Q5Z749 (Auxin-responsive protein IAA21 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA21 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.7e-63
Identity = 150/315 (47.62%), Postives = 180/315 (57.14%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGS--DSPLRDDGLD 69
           M+ P E DYIGL+ +  +                   ATELRLGLPG+  ++     G  
Sbjct: 1   MAPPQERDYIGLSPAAAA-----------------ALATELRLGLPGTAEEAESEGGGGG 60

Query: 70  DTNGYPHKTSV---SGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENK 129
            T+  P    +    GAKRGF+ AI                      GG    R      
Sbjct: 61  GTDAAPLTLELLPKGGAKRGFADAI---------------------VGGPAGQR------ 120

Query: 130 TQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPP--K 189
                   +G K   ++      EEK +   PAAKAQVVGWPPIRS+RKN+MA   P  K
Sbjct: 121 -----REAAGGKAAAAAAEAEEEEEKKKAQAPAAKAQVVGWPPIRSYRKNTMAMSQPALK 180

Query: 190 NTDDADGKM--GSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGS 249
             DD + K    SGCLYVKVSMDGAPYLRKVDLK Y +Y +LS AL KMFS FT+GH  S
Sbjct: 181 GKDDGEAKQAPASGCLYVKVSMDGAPYLRKVDLKMYKNYKELSLALEKMFSCFTVGHGES 240

Query: 250 NGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEA 309
           NG   RD L++ RLMDL +G+E VLTYEDKD DWMLVGDVPW MFT+SC R+RIMK S+A
Sbjct: 241 NGKSGRDGLSDCRLMDLKNGTELVLTYEDKDEDWMLVGDVPWRMFTDSCRRLRIMKGSDA 266

Query: 310 IGLAPRAMEKRRNLN 316
           +GLAPRA +K +N N
Sbjct: 301 VGLAPRATDKSKNRN 266

BLAST of Carg12216 vs. ExPASy TrEMBL
Match: A0A6J1F0T2 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111441117 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 303/306 (99.02%), Postives = 304/306 (99.35%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT
Sbjct: 1   MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSP VVTEN TQPPA
Sbjct: 61  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPGVVTENMTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 189
           SPVSGAKDGIS+LPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG
Sbjct: 121 SPVSGAKDGISTLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 180

Query: 190 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 249
           KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL
Sbjct: 181 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 240

Query: 250 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 309
           NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME
Sbjct: 241 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 300

Query: 310 KRRNLN 316
           KRRNLN
Sbjct: 301 KRRNLN 306

BLAST of Carg12216 vs. ExPASy TrEMBL
Match: A0A6J1KKY6 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111495511 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.4e-157
Identity = 288/308 (93.51%), Postives = 290/308 (94.16%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLTD       SEMSSD RNNPGLNL+ATELRLGLPGSDSPL    LDDT
Sbjct: 1   MSIPLEHDYIGLTD-------SEMSSDRRNNPGLNLRATELRLGLPGSDSPL----LDDT 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLP--ASAGSEPDSTKTGGLFSPRVVTENKTQP 129
           NGYP KTSVSGAKRGFSVAIDRASS WVLP  ASAGSEPDSTKTGGLFSPRVVTENKTQP
Sbjct: 61  NGYPLKTSVSGAKRGFSVAIDRASSNWVLPASASAGSEPDSTKTGGLFSPRVVTENKTQP 120

Query: 130 PASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDA 189
           PASPVSGAKDGIS+LPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSM TQPPKNTDDA
Sbjct: 121 PASPVSGAKDGISTLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMTTQPPKNTDDA 180

Query: 190 DGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRD 249
           DGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRD
Sbjct: 181 DGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRD 240

Query: 250 ALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRA 309
            LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRA
Sbjct: 241 VLNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRA 297

Query: 310 MEKRRNLN 316
           MEKRRNLN
Sbjct: 301 MEKRRNLN 297

BLAST of Carg12216 vs. ExPASy TrEMBL
Match: A0A5D3BFM3 (Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold369G00160 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.1e-149
Identity = 269/307 (87.62%), Postives = 284/307 (92.51%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLT+SVPSLE+SE SSD RNN GLNLKATELRLGLPGS+SP+RDDG +D 
Sbjct: 1   MSIPLEHDYIGLTESVPSLESSEKSSDKRNNAGLNLKATELRLGLPGSESPVRDDGFEDN 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NG+ HK+ VSGAKRGFS+AIDRAS+KWVLPASAGSE DS+  GGLFSPR V ENKTQPPA
Sbjct: 61  NGFLHKSPVSGAKRGFSIAIDRASAKWVLPASAGSEADSSTNGGLFSPRGVNENKTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLL-EEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD 189
           S V G KDGIS   KP+L EEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD
Sbjct: 121 SAVPGVKDGISPSAKPVLNEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD 180

Query: 190 GKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDA 249
           GK+GSGCLYVKVSMDGAPYLRKVDLKTY SY+DLSSAL KMFSSFTIGHCGSNG+PNRDA
Sbjct: 181 GKLGSGCLYVKVSMDGAPYLRKVDLKTYVSYVDLSSALEKMFSSFTIGHCGSNGVPNRDA 240

Query: 250 LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM 309
           LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM
Sbjct: 241 LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM 300

Query: 310 EKRRNLN 316
           EK +N N
Sbjct: 301 EKCKNRN 307

BLAST of Carg12216 vs. ExPASy TrEMBL
Match: A0A0A0LT68 (Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G207820 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.2e-149
Identity = 269/306 (87.91%), Postives = 282/306 (92.16%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLT+SVPSLE SE SSD RN+ GLNLKATELRLGLPGS+SP RDDG +D 
Sbjct: 1   MSIPLEHDYIGLTESVPSLENSEKSSDKRNSAGLNLKATELRLGLPGSESPGRDDGFEDK 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NG+ HK+SVSGAKRGFS+AIDRAS+KWVLPASAGSE DS+  GGLFSPR V ENKTQPPA
Sbjct: 61  NGFLHKSSVSGAKRGFSIAIDRASAKWVLPASAGSEADSSTNGGLFSPRGVNENKTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 189
           S VSG KDGIS   KPL EEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG
Sbjct: 121 SAVSGVKDGISPSAKPLHEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDADG 180

Query: 190 KMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDAL 249
           K+GSGCLYVKVSMDGAPYLRKVDLKTY SY+DLSSAL KMFSSFTIGH GSNG+PNRDAL
Sbjct: 181 KLGSGCLYVKVSMDGAPYLRKVDLKTYVSYVDLSSALEKMFSSFTIGHYGSNGVPNRDAL 240

Query: 250 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 309
           NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME
Sbjct: 241 NESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAME 300

Query: 310 KRRNLN 316
           K +N N
Sbjct: 301 KCKNRN 306

BLAST of Carg12216 vs. ExPASy TrEMBL
Match: A0A1S3CHQ1 (Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103501033 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.2e-149
Identity = 269/307 (87.62%), Postives = 283/307 (92.18%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGLDDT 69
           MSIPLEHDYIGLT+SVPSLE+SE SSD RNN GLNLKATELRLGLPGS+SP RDDG +D 
Sbjct: 1   MSIPLEHDYIGLTESVPSLESSEKSSDKRNNAGLNLKATELRLGLPGSESPGRDDGFEDN 60

Query: 70  NGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPRVVTENKTQPPA 129
           NG+ HK+ VSGAKRGFS+AIDRAS+KWVLPASAGSE DS+  GGLFSPR V ENKTQPPA
Sbjct: 61  NGFLHKSPVSGAKRGFSIAIDRASAKWVLPASAGSEADSSTNGGLFSPRGVNENKTQPPA 120

Query: 130 SPVSGAKDGISSLPKPLL-EEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD 189
           S V G KDGIS   KP+L EEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD
Sbjct: 121 SAVPGVKDGISPSAKPVLNEEKPQLSPPAAKAQVVGWPPIRSFRKNSMATQPPKNTDDAD 180

Query: 190 GKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFTIGHCGSNGIPNRDA 249
           GK+GSGCLYVKVSMDGAPYLRKVDLKTY SY+DLSSAL KMFSSFTIGHCGSNG+PNRDA
Sbjct: 181 GKLGSGCLYVKVSMDGAPYLRKVDLKTYVSYVDLSSALEKMFSSFTIGHCGSNGVPNRDA 240

Query: 250 LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM 309
           LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM
Sbjct: 241 LNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRIMKSSEAIGLAPRAM 300

Query: 310 EKRRNLN 316
           EK +N N
Sbjct: 301 EKCKNRN 307

BLAST of Carg12216 vs. TAIR 10
Match: AT4G29080.1 (phytochrome-associated protein 2 )

HSP 1 Score: 301.2 bits (770), Expect = 9.7e-82
Identity = 186/321 (57.94%), Postives = 219/321 (68.22%), Query Frame = 0

Query: 10  MSIPLEHDYIGLTDSVPSLETSEMSSDTR---NNPGLNLKATELRLGLPGSDSPLRDDGL 69
           +S+  EHDYIGL++  P++E + MS  T+   NN GLN KATELRLGLPGS+SP R   +
Sbjct: 3   VSVAAEHDYIGLSE-FPTMEATTMSDKTKTRDNNNGLNFKATELRLGLPGSESPER---V 62

Query: 70  DDTNGYPHKTS--VSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGGLFSPR--VVTE 129
           D      +K+S  VSGAKR FS AI+  S+KWV   S GS   +   G    PR  VV +
Sbjct: 63  DSRFLALNKSSCPVSGAKRVFSDAIN-DSNKWVF--SPGSTTATGDVGSGSGPRTSVVKD 122

Query: 130 NKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRKNSMA---TQ 189
            K+     P             P+ E+K   + PA+KAQVVGWPPIRSFRKNSMA   +Q
Sbjct: 123 GKSTTFTKPA-----------VPVKEKKSSATAPASKAQVVGWPPIRSFRKNSMASSQSQ 182

Query: 190 PPKN---TDDADGKMG--SGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSFT 249
            P N   T++A+ K G    CLYVKVSM+GAPYLRK+DLKTY SYL+LSSAL KMFS FT
Sbjct: 183 KPGNNSETEEAEAKSGPEQPCLYVKVSMEGAPYLRKIDLKTYKSYLELSSALEKMFSCFT 242

Query: 250 IGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMRI 309
           IG  GS+G   RD LNESRL DLL GSEYV+TYEDKD DWMLVGDVPWEMF  SC ++RI
Sbjct: 243 IGQFGSHGGCGRDGLNESRLTDLLRGSEYVVTYEDKDSDWMLVGDVPWEMFICSCKKLRI 302

Query: 310 MKSSEAIGLAPRAMEKRRNLN 316
           MKSSEAIGLAPR MEK R+ N
Sbjct: 303 MKSSEAIGLAPRVMEKCRSRN 305

BLAST of Carg12216 vs. TAIR 10
Match: AT5G65670.2 (indole-3-acetic acid inducible 9 )

HSP 1 Score: 271.6 bits (693), Expect = 8.2e-73
Identity = 155/321 (48.29%), Postives = 203/321 (63.24%), Query Frame = 0

Query: 15  EHDYIGLTD--SVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLRDDGL------ 74
           EH+Y+GL+D  SV S   S ++ D +    ++LKATEL LGLPGS SP RD  L      
Sbjct: 33  EHNYLGLSDCSSVGSSTLSPLAEDDKAT--ISLKATELTLGLPGSQSPARDTELNLLSPA 92

Query: 75  --------------DDTNGYPHKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTG 134
                         D+      K + SG KRGFS  +D+ +               T+  
Sbjct: 93  KLDEKPFFPLLPSKDEICSSSQKNNASGNKRGFSDTMDQFAE--------AKSSVYTEKN 152

Query: 135 GLFSPRVVTENKTQPPASPVSGAKDGISSLPK-PLLEEKPQLSPPAAKAQVVGWPPIRSF 194
            +F     T++ T+         KD   ++PK          SPPAAKAQ+VGWPP+RS+
Sbjct: 153 WMFPEAAATQSVTK---------KDVPQNIPKGQSSTTNNSSSPPAAKAQIVGWPPVRSY 212

Query: 195 RKNSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFS 254
           RKN++AT   KN+D+ DG+ GSG L+VKVSMDGAPYLRKVDL++Y +Y +LSSAL KMF+
Sbjct: 213 RKNTLAT-TCKNSDEVDGRPGSGALFVKVSMDGAPYLRKVDLRSYTNYGELSSALEKMFT 272

Query: 255 SFTIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTR 313
           +FT+G CGSNG   +D L+E++L DLL+G +YVLTYEDKDGDWMLVGDVPWEMF + C +
Sbjct: 273 TFTLGQCGSNGAAGKDMLSETKLKDLLNGKDYVLTYEDKDGDWMLVGDVPWEMFIDVCKK 332

BLAST of Carg12216 vs. TAIR 10
Match: AT2G22670.1 (indoleacetic acid-induced protein 8 )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 161/320 (50.31%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 15  EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLR--DDGL------ 74
           E +Y+GL+D   S   S    +      LN KATELRLGLP S SP R  D GL      
Sbjct: 21  ERNYLGLSDC--SSVDSSTIPNVVGKSNLNFKATELRLGLPESQSPERETDFGLLSPRTP 80

Query: 75  DDTNGYP-------------HKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGG 134
           D+   +P             HK  VSG KRGF+   D  S         GS         
Sbjct: 81  DEKLLFPLLPSKDNGSATTGHKNVVSGNKRGFADTWDEFS------GVKGSVRPGGGINM 140

Query: 135 LFSPRVVTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRK 194
           + SP+V   +K+       S AK G++             + PAAKAQVVGWPPIRS+RK
Sbjct: 141 MLSPKVKDVSKSIQEER--SHAKGGLN-------------NAPAAKAQVVGWPPIRSYRK 200

Query: 195 NSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSF 254
           N+MA+   KNTD+ DGK G G L+VKVSMDGAPYLRKVDL+TY SY  LSSAL KMFS F
Sbjct: 201 NTMASSTSKNTDEVDGKPGLGVLFVKVSMDGAPYLRKVDLRTYTSYQQLSSALEKMFSCF 260

Query: 255 TIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMR 314
           T+G CG +G   R+ ++E +L DLLHGSE+VLTYEDKDGDWMLVGDVPWE+FTE+C +++
Sbjct: 261 TLGQCGLHGAQGRERMSEIKLKDLLHGSEFVLTYEDKDGDWMLVGDVPWEIFTETCQKLK 317

BLAST of Carg12216 vs. TAIR 10
Match: AT2G22670.3 (indoleacetic acid-induced protein 8 )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 161/320 (50.31%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 15  EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLR--DDGL------ 74
           E +Y+GL+D   S   S    +      LN KATELRLGLP S SP R  D GL      
Sbjct: 21  ERNYLGLSDC--SSVDSSTIPNVVGKSNLNFKATELRLGLPESQSPERETDFGLLSPRTP 80

Query: 75  DDTNGYP-------------HKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGG 134
           D+   +P             HK  VSG KRGF+   D  S         GS         
Sbjct: 81  DEKLLFPLLPSKDNGSATTGHKNVVSGNKRGFADTWDEFS------GVKGSVRPGGGINM 140

Query: 135 LFSPRVVTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRK 194
           + SP+V   +K+       S AK G++             + PAAKAQVVGWPPIRS+RK
Sbjct: 141 MLSPKVKDVSKSIQEER--SHAKGGLN-------------NAPAAKAQVVGWPPIRSYRK 200

Query: 195 NSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSF 254
           N+MA+   KNTD+ DGK G G L+VKVSMDGAPYLRKVDL+TY SY  LSSAL KMFS F
Sbjct: 201 NTMASSTSKNTDEVDGKPGLGVLFVKVSMDGAPYLRKVDLRTYTSYQQLSSALEKMFSCF 260

Query: 255 TIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMR 314
           T+G CG +G   R+ ++E +L DLLHGSE+VLTYEDKDGDWMLVGDVPWE+FTE+C +++
Sbjct: 261 TLGQCGLHGAQGRERMSEIKLKDLLHGSEFVLTYEDKDGDWMLVGDVPWEIFTETCQKLK 317

BLAST of Carg12216 vs. TAIR 10
Match: AT2G22670.4 (indoleacetic acid-induced protein 8 )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 161/320 (50.31%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 15  EHDYIGLTDSVPSLETSEMSSDTRNNPGLNLKATELRLGLPGSDSPLR--DDGL------ 74
           E +Y+GL+D   S   S    +      LN KATELRLGLP S SP R  D GL      
Sbjct: 38  ERNYLGLSDC--SSVDSSTIPNVVGKSNLNFKATELRLGLPESQSPERETDFGLLSPRTP 97

Query: 75  DDTNGYP-------------HKTSVSGAKRGFSVAIDRASSKWVLPASAGSEPDSTKTGG 134
           D+   +P             HK  VSG KRGF+   D  S         GS         
Sbjct: 98  DEKLLFPLLPSKDNGSATTGHKNVVSGNKRGFADTWDEFS------GVKGSVRPGGGINM 157

Query: 135 LFSPRVVTENKTQPPASPVSGAKDGISSLPKPLLEEKPQLSPPAAKAQVVGWPPIRSFRK 194
           + SP+V   +K+       S AK G++             + PAAKAQVVGWPPIRS+RK
Sbjct: 158 MLSPKVKDVSKSIQEER--SHAKGGLN-------------NAPAAKAQVVGWPPIRSYRK 217

Query: 195 NSMATQPPKNTDDADGKMGSGCLYVKVSMDGAPYLRKVDLKTYGSYLDLSSALGKMFSSF 254
           N+MA+   KNTD+ DGK G G L+VKVSMDGAPYLRKVDL+TY SY  LSSAL KMFS F
Sbjct: 218 NTMASSTSKNTDEVDGKPGLGVLFVKVSMDGAPYLRKVDLRTYTSYQQLSSALEKMFSCF 277

Query: 255 TIGHCGSNGIPNRDALNESRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFTESCTRMR 314
           T+G CG +G   R+ ++E +L DLLHGSE+VLTYEDKDGDWMLVGDVPWE+FTE+C +++
Sbjct: 278 TLGQCGLHGAQGRERMSEIKLKDLLHGSEFVLTYEDKDGDWMLVGDVPWEIFTETCQKLK 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021869.11.5e-177100.00Auxin-responsive protein IAA27 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023531223.11.2e-17199.02auxin-responsive protein IAA27-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6587978.12.7e-17197.46Auxin-responsive protein IAA27, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933814.16.1e-17199.02auxin-responsive protein IAA27-like [Cucurbita moschata][more]
XP_023531224.15.9e-16694.92auxin-responsive protein IAA27-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9ZSY81.4e-8057.94Auxin-responsive protein IAA27 OS=Arabidopsis thaliana OX=3702 GN=IAA27 PE=1 SV=... [more]
Q388262.2e-7050.31Auxin-responsive protein IAA8 OS=Arabidopsis thaliana OX=3702 GN=IAA8 PE=1 SV=1[more]
Q388273.7e-7047.99Auxin-responsive protein IAA9 OS=Arabidopsis thaliana OX=3702 GN=IAA9 PE=1 SV=1[more]
P0C1321.0e-6452.11Auxin-responsive protein IAA30 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA30... [more]
Q5Z7495.7e-6347.62Auxin-responsive protein IAA21 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA21... [more]
Match NameE-valueIdentityDescription
A0A6J1F0T23.0e-17199.02Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111441117 PE=3 SV=1[more]
A0A6J1KKY62.4e-15793.51Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111495511 PE=3 SV=1[more]
A0A5D3BFM31.1e-14987.62Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0LT684.2e-14987.91Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_1G207820 PE=3 SV=1[more]
A0A1S3CHQ14.2e-14987.62Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103501033 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29080.19.7e-8257.94phytochrome-associated protein 2 [more]
AT5G65670.28.2e-7348.29indole-3-acetic acid inducible 9 [more]
AT2G22670.11.5e-7150.31indoleacetic acid-induced protein 8 [more]
AT2G22670.31.5e-7150.31indoleacetic acid-induced protein 8 [more]
AT2G22670.41.5e-7150.31indoleacetic acid-induced protein 8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033389AUX/IAA domainPFAMPF02309AUX_IAAcoord: 48..306
e-value: 5.3E-85
score: 285.1
NoneNo IPR availableGENE3D3.10.20.90coord: 190..308
e-value: 3.1E-42
score: 144.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..161
NoneNo IPR availablePANTHERPTHR31734:SF150AUXIN-RESPONSIVE PROTEIN IAA27coord: 23..307
NoneNo IPR availableSUPERFAMILY54277CAD & PB1 domainscoord: 191..296
IPR003311AUX/IAA proteinPANTHERPTHR31734AUXIN-RESPONSIVE PROTEIN IAA17coord: 23..307
IPR000270PB1 domainPROSITEPS51745PB1coord: 195..297
score: 23.763987

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12216-RACarg12216-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding