Carg12171 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12171
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	sodium/pyruvate cotransporter BASS2, chloroplastic
Location	Carg_Chr11: 2553228 .. 2554471 (+)
RNA-Seq Expression	Carg12171
Synteny	Carg12171

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATCTTCTTTCCATATTGGGCAAGAATTCATTTTCTAATTGAGCGCCACTCTACTTCGATTCAGCTACCATTCTGCAATTTCAACTGCAGCTACATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTGTATGTTTTAGTTATCTACTTTAGGGTCACTCTCTATCTGGGCGCGCTGATTTTTGCTTCTATTTTCAAGTTTCATGAAGACACGGCTTAAATTTTTGCTTCTTTTTGTCTTTAGATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGTATGATGTTAAACATGTGACTATTGCTTCATATTGTCTAGAATTCCTTTCATGAAAGTTCATTCCCCCTTATATTTAGATTTACTTGTTCTTCCTGATGCTTATTGGTTACGTTCATAGATTTAGTTAGATTGGAGACTATACCTTATAATAAATACATATTGAACTATCAACTCAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTAATGCTTATTAGTTCTGTTTTTGTGAATCTGGATTTAGTGCATGGTTGTGTTCTGGAGCTTTGCCTTTTAATTCCTGAGTTTGCCACCTATGAACAGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAATTGCTCATTCTTGGTTCTTCTTGATTGATGATTGGTGAATCTACTGGTACTCATCAGGCTCCCCTTGCAGGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTATTTAGCATCTATTTTCCTGGTGCAGGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

mRNA sequence

CAATCTTCTTTCCATATTGGGCAAGAATTCATTTTCTAATTGAGCGCCACTCTACTTCGATTCAGCTACCATTCTGCAATTTCAACTGCAGCTACATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

Coding sequence (CDS)

ATGGCGTCATTTTCCAAATTCGAGTACACAGATTGTAGAACACACAAATTTGATGCTCTCTACAGGCAAACCTACGATTTCTCCGCGAAGAAGCCTACAGCCCATCTGTATATAAGGGGAAAATTATGTTCATCTCACAATGCAAGAAGTTTAGTAGCTCAGTGCAAACTGCTTAAGCCGATCGTTTTTTCTGGCTTTCCTTCTGCAATCATCTCAAGGAAGCTCCAAATTCTGTGCAAAGCTGCAAATGATGTCTCTGGAGATATCCCTGATGGTAATTCTCCAAGTGGAATGAGTCTGTACGAAAGAACTATTGAAACTTTAACCACACTTTTTCCTCTGTGGGTTATCCTTGGAACCATTATCGGCATCTACAAACCTGCTGCAGTAACTTGGTTGGAGACTGATCTTTTTACCCTGGGATTGGGTTTTCTTATGCTTTCGATGGGATTGACATTGACATTTGAGGATTTCAGGCGTTGTTTGAGAAATCCATGGACTGTAAGAGACATCCTTCTACTAACCAAATGGATGTACTTAAATAGTTGTGTTGGTGTAGGATTTCTTGCCCAGTACTTAATTAAACCTCTGCTTGGCTTTACTATCTCCATGGTAATAACTATGATGAAATCTTATAGCTCAATATCATGCTGA

Protein sequence

MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKPIVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMVITMMKSYSSISC

Homology

[Close]

BLAST of Carg12171 vs. NCBI nr
Match: KAG7021824.1 (Sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 436.8 bits (1122), Expect = 1.1e-118
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITMMKSYSSISC 218
           NSCVGVGFLAQYLIKPLLGFTISMVITMMKSYSSISC
Sbjct: 181 NSCVGVGFLAQYLIKPLLGFTISMVITMMKSYSSISC 217

[Close]

BLAST of Carg12171 vs. NCBI nr
Match: KAG6587936.1 (Sodium/pyruvate cotransporter BASS2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.8 bits (930), Expect = 2.0e-96
Identity = 185/208 (88.94%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYI GKLCSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYISGKLCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. NCBI nr
Match: XP_022932828.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 183/208 (87.98%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTH FDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHNFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIP+GNSPSG+SLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPEGNSPSGVSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. NCBI nr
Match: XP_023531206.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 184/208 (88.46%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGK CSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKSCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPD NSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDDNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. NCBI nr
Match: XP_023001525.1 (sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 359.0 bits (920), Expect = 2.9e-95
Identity = 183/208 (87.98%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDA YRQTYDFSAKKPT HLYIRGKLCSSHNARSLV+QCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDAPYRQTYDFSAKKPTDHLYIRGKLCSSHNARSLVSQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.3e-42
Identity = 107/208 (51.44%), Postives = 133/208 (63.94%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS S+   TD R  +       ++  S  +   HL     +  S++  SL  Q    KP
Sbjct: 1   MASISRILPTDGRLSQ--CRINTSWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNS--KP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           I       A  SR+  ++CKAA  VSGD+P+ ++P  +S YE+ IE LTTLFPLWVILGT
Sbjct: 61  ISPVFALEATSSRR--VVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           ++GI+KP+ VTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 LVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQY+IKP+LGF I+M + +
Sbjct: 181 ---VGVGFLAQYMIKPILGFLIAMTLKL 185

[Close]

BLAST of Carg12171 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.9e-41
Identity = 86/136 (63.24%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 73  RKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTW 132
           R  Q+LCKA  ++S ++P+ + PS  + YE+ +E LTTLFP+WVILGTIIGIYKP+ VTW
Sbjct: 76  RSCQLLCKAEANISSNLPE-SIPSEANQYEKIVELLTTLFPVWVILGTIIGIYKPSMVTW 135

Query: 133 LETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQY 192
           LETDLFT+GLGFLMLSMGLTLTFEDFRRC+RNPWT                VGVGFLAQY
Sbjct: 136 LETDLFTVGLGFLMLSMGLTLTFEDFRRCMRNPWT----------------VGVGFLAQY 194

Query: 193 LIKPLLGFTISMVITM 209
           LIKP+LGF I+M + +
Sbjct: 196 LIKPMLGFAIAMTLKL 194

[Close]

BLAST of Carg12171 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.1e-10
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 86  SGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFL 145
           S D+P     S     E   E ++T FP+WV LG ++G+ +P+   W+  +   +GL   
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIVGLTIT 134

Query: 146 MLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMV 205
           ML MG+TLT +D R  L  P          K ++       GFL QY + PL  F +S +
Sbjct: 135 MLGMGMTLTLDDLRGALSMP----------KELF------AGFLLQYSVMPLSAFFVSKL 187

Query: 206 ITMMKSYSS 215
           + +   Y++
Sbjct: 195 LNLPPHYAA 187

[Close]

BLAST of Carg12171 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-07
Identity = 42/148 (28.38%), Postives = 65/148 (43.92%), Query Frame = 0


Query: 81  AANDVSGDIPDGNSPS-------GMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWL 140
           AA+     IP G S +          L  +  E L+  FP+WV     + +++P A  W+
Sbjct: 68  AADPAPSKIPGGGSGALEAGVVGWRDLLLQVGEVLSLGFPVWVASACAVALWRPPAFLWV 127

Query: 141 ETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYL 200
                 +G+ F ML MG+TLT +D +  L  P  +                  GFL QY 
Sbjct: 128 SPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKELAS----------------GFLLQYS 187

Query: 201 IKPLLGFTISMVITMMKSYSS----ISC 218
           + PL GF IS ++ +   Y++    +SC
Sbjct: 188 VMPLSGFLISKLLNLPSYYAAGLILVSC 199

[Close]

BLAST of Carg12171 vs. ExPASy Swiss-Prot
Match: Q8RXE8 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.4e-06
Identity = 31/104 (29.81%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 109 TTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTV 168
           + L P  V L  +  +  P + TW+  DL+   LG +MLS+G+ L+ +DF    + P   
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVP- 171

Query: 169 RDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMVITMMKSY 213
                          + VGF+AQY++KPLLG  ++    M +++
Sbjct: 172 ---------------LSVGFVAQYVLKPLLGVLVANAFGMPRTF 199

[Close]

BLAST of Carg12171 vs. ExPASy TrEMBL
Match: A0A6J1F2U8 (sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439284 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.3e-96
Identity = 183/208 (87.98%), Postives = 187/208 (89.90%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTH FDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHNFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIP+GNSPSG+SLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPEGNSPSGVSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. ExPASy TrEMBL
Match: A0A6J1KLF3 (sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495636 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.4e-95
Identity = 183/208 (87.98%), Postives = 186/208 (89.42%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MASFSKFEYTDCRTHKFDA YRQTYDFSAKKPT HLYIRGKLCSSHNARSLV+QCKLLKP
Sbjct: 1   MASFSKFEYTDCRTHKFDAPYRQTYDFSAKKPTDHLYIRGKLCSSHNARSLVSQCKLLKP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           IVFSG PSAI SRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT
Sbjct: 61  IVFSGSPSAITSRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQYLIKPLLGFTISM + +
Sbjct: 181 ---VGVGFLAQYLIKPLLGFTISMTLKL 192

[Close]

BLAST of Carg12171 vs. ExPASy TrEMBL
Match: A0A6J1CTH3 (sodium/pyruvate cotransporter BASS2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014598 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.5e-70
Identity = 144/210 (68.57%), Postives = 164/210 (78.10%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS SKF + DCR  + DA+ R++Y  SA KP AHL ++GKLC S N RSLVAQ  LL+P
Sbjct: 29  MASISKFAFKDCRLQRNDAICRRSYSASAMKPQAHLDVKGKLCLSDNGRSLVAQFMLLRP 88

Query: 61  IVFSGFPSAI--ISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVIL 120
           ++ S  P A+   SRKLQ+LCKAAND SG++P GNSPSG SLYERTIETLTTLFPLWVIL
Sbjct: 89  VLSSASPCAVGQTSRKLQMLCKAANDASGNVP-GNSPSGTSLYERTIETLTTLFPLWVIL 148

Query: 121 GTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWM 180
           GT+IGIY+PAAVTWLETDLFT+ LGFLMLSMGLTLTFEDFRRCLRNPWT           
Sbjct: 149 GTVIGIYRPAAVTWLETDLFTVALGFLMLSMGLTLTFEDFRRCLRNPWT----------- 208

Query: 181 YLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                VGVGFLAQYLIKP+LGFTISM + +
Sbjct: 209 -----VGVGFLAQYLIKPMLGFTISMTLKL 221

[Close]

BLAST of Carg12171 vs. ExPASy TrEMBL
Match: A0A1S3CKL3 (sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501527 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-66
Identity = 142/212 (66.98%), Postives = 159/212 (75.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARS--LVAQCKLL 60
           MAS SKF + DC   +FDALY + + FSA K  +HL  RGKLC S + R   +VAQ  LL
Sbjct: 1   MASISKFAFNDCSIMRFDALYNRNFHFSAGKFQSHLDFRGKLCLSDSGRRRLVVAQSMLL 60

Query: 61  KPIVFSGFPSAI--ISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
           KP+V S  P AI   SRKLQILCKAA+DVSG+I  G+SP GMS YER IETLTTLFP+WV
Sbjct: 61  KPLVSSASPCAIGVTSRKLQILCKAASDVSGEIVPGDSPDGMSTYERVIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTIIGIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTIIGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFVIAMTLKL 196

[Close]

BLAST of Carg12171 vs. ExPASy TrEMBL
Match: A0A0A0LZK6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G404660 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.4e-65
Identity = 137/212 (64.62%), Postives = 159/212 (75.00%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARS--LVAQCKLL 60
           MAS SK  + DCR  +FDAL+ + + FS  K  +HL +RGKLC S + R   +VAQ  LL
Sbjct: 1   MASISKLAFNDCRMQRFDALHNRNFHFSPGKLQSHLDVRGKLCLSDSRRRSLVVAQSMLL 60

Query: 61  KPIVFSGFPSA--IISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWV 120
           KP+V S  P A  + SRKLQILC+AA+DVSG+I  G+SP GMS YER IETLTTLFP+WV
Sbjct: 61  KPLVSSASPCAVRVTSRKLQILCEAASDVSGEIVPGDSPDGMSTYERVIETLTTLFPVWV 120

Query: 121 ILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTK 180
           ILGTI+GIYKPAAVTWLETDLFT+GLGFLMLSMGLTLTFEDFRRCLRNPWT         
Sbjct: 121 ILGTILGIYKPAAVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRCLRNPWT--------- 180

Query: 181 WMYLNSCVGVGFLAQYLIKPLLGFTISMVITM 209
                  VGVGFLAQYLIKP+LGF I+M + +
Sbjct: 181 -------VGVGFLAQYLIKPMLGFVIAMTLKL 196

[Close]

BLAST of Carg12171 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 173.7 bits (439), Expect = 1.6e-43
Identity = 107/208 (51.44%), Postives = 133/208 (63.94%), Query Frame = 0


Query: 1   MASFSKFEYTDCRTHKFDALYRQTYDFSAKKPTAHLYIRGKLCSSHNARSLVAQCKLLKP 60
           MAS S+   TD R  +       ++  S  +   HL     +  S++  SL  Q    KP
Sbjct: 1   MASISRILPTDGRLSQ--CRINTSWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNS--KP 60

Query: 61  IVFSGFPSAIISRKLQILCKAANDVSGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGT 120
           I       A  SR+  ++CKAA  VSGD+P+ ++P  +S YE+ IE LTTLFPLWVILGT
Sbjct: 61  ISPVFALEATSSRR--VVCKAAAGVSGDLPE-STPKELSQYEKIIELLTTLFPLWVILGT 120

Query: 121 IIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYL 180
           ++GI+KP+ VTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT             
Sbjct: 121 LVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWT------------- 180

Query: 181 NSCVGVGFLAQYLIKPLLGFTISMVITM 209
              VGVGFLAQY+IKP+LGF I+M + +
Sbjct: 181 ---VGVGFLAQYMIKPILGFLIAMTLKL 185

[Close]

BLAST of Carg12171 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 66.2 bits (160), Expect = 3.6e-11
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 86  SGDIPDGNSPSGMSLYERTIETLTTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFL 145
           S D+P     S     E   E ++T FP+WV LG ++G+ +P+   W+  +   +GL   
Sbjct: 75  SNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTIVGLTIT 134

Query: 146 MLSMGLTLTFEDFRRCLRNPWTVRDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMV 205
           ML MG+TLT +D R  L  P          K ++       GFL QY + PL  F +S +
Sbjct: 135 MLGMGMTLTLDDLRGALSMP----------KELF------AGFLLQYSVMPLSAFFVSKL 187

Query: 206 ITMMKSYSS 215
           + +   Y++
Sbjct: 195 LNLPPHYAA 187

[Close]

BLAST of Carg12171 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 53.5 bits (127), Expect = 2.4e-07
Identity = 31/104 (29.81%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 109 TTLFPLWVILGTIIGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNPWTV 168
           + L P  V L  +  +  P + TW+  DL+   LG +MLS+G+ L+ +DF    + P   
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVP- 171

Query: 169 RDILLLTKWMYLNSCVGVGFLAQYLIKPLLGFTISMVITMMKSY 213
                          + VGF+AQY++KPLLG  ++    M +++
Sbjct: 172 ---------------LSVGFVAQYVLKPLLGVLVANAFGMPRTF 199

The following BLAST results are available for this feature:

BLAST of Carg12171 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021824.1	1.1e-118	100.00	Sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita argyrosperma subsp...	[more]
KAG6587936.1	2.0e-96	88.94	Sodium/pyruvate cotransporter BASS2, chloroplastic, partial [Cucurbita argyrospe...	[more]
XP_022932828.1	1.3e-95	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita moschata]	[more]
XP_023531206.1	1.3e-95	88.46	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_023001525.1	2.9e-95	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic [Cucurbita maxima]	[more]

BLAST of Carg12171 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1EBV7	2.3e-42	51.44	Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
Q5VRB2	1.9e-41	63.24	Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su...	[more]
Q93YR2	5.1e-10	31.01	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis tha...	[more]
Q7XVB3	1.1e-07	28.38	Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa su...	[more]
Q8RXE8	3.4e-06	29.81	Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis tha...	[more]

BLAST of Carg12171 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2U8	6.3e-96	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KLF3	1.4e-95	87.98	sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CTH3	3.5e-70	68.57	sodium/pyruvate cotransporter BASS2, chloroplastic OS=Momordica charantia OX=367...	[more]
A0A1S3CKL3	1.8e-66	66.98	sodium/pyruvate cotransporter BASS2, chloroplastic OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LZK6	4.4e-65	64.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G404660 PE=3 SV=1	[more]

BLAST of Carg12171 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26900.1	1.6e-43	51.44	Sodium Bile acid symporter family	[more]
AT1G78560.1	3.6e-11	31.01	Sodium Bile acid symporter family	[more]
AT3G25410.1	2.4e-07	29.81	Sodium Bile acid symporter family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038770	Sodium/solute symporter superfamily	GENE3D	1.20.1530.20		coord: 95..214 e-value: 8.3E-17 score: 63.2
IPR002657	Bile acid:sodium symporter/arsenical resistance protein Acr3	PFAM	PF01758	SBF	coord: 139..209 e-value: 5.5E-8 score: 32.7
IPR004710	Bile acid:sodium symporter	PANTHER	PTHR10361	SODIUM-BILE ACID COTRANSPORTER	coord: 52..210
None	No IPR available	PANTHER	PTHR10361:SF62	SODIUM/PYRUVATE COTRANSPORTER BASS2, CHLOROPLASTIC	coord: 52..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12171-RA	Carg12171-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane