Carg12161 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12161
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 6.2-like
LocationCarg_Chr11: 2506936 .. 2510478 (+)
RNA-Seq ExpressionCarg12161
SyntenyCarg12161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATTCCTTTCAGAATCTCTGCCTACAAAACCAACAACTCCCTCACCACTTTCCACACTCACTTTCAACCCTTTCAAAAACTAAACCTTCTTTTACCTCATCTTCTCCATAGCTGCTACCTGCAAAGCTTCAAAAATGGTTGGTTCTTTTGAGAAACAGAGCTCCAATGGAGCCTTACTTTCATTTTCACCTCCATTTTTGTCTTATCGCTAATGGGTGAGCTTAAAAATTGTGTCTTTTTTTGTTCTGTTTGTTAGGATGGGAAGCTAAGTTGTAGAGTGGCTGATGCTGTGGATTACAAAGGCTTTCCTGCTGATAAGTCTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTGTGTAAATACTTAGCTCTTTAAGCTATTATGAAATGGCATTCATCTTAAAGTTGTTTCTGATATGATCTTATTAGCAGAGATTCAAGCCGGTATGATATTGCATGCAAATTTTCCAGGGAAAATATGGAGATGAGATTTGTTTTTTTATGATTCATTAGATTCTGTTTCAACTACAAAACTCCAAATTTTAAAAAGTGGAAGAACAGAAGAACTGAGAGGGACATAACGTAGATGGCTGCTTATTGGTGATGCCTTTTTGTGTTCATATGGGAATAAATGACATAATCTTATGTCACTTCATCACAAGCTTTTTAAGATAATATCACTTTTTTTTTTTTTTTTTTTTTCCTTTTCTTTGAAAAGATAAAGATTCAGTTTGGAAAATATTTGATAATTTTTCTTTAAAAAGATGGAGGATTGCTTGTTCCTTATGAGCAAAAATGGAGTTCTTGTTCTCTTGTTCGCTGTTCCAAAAGAACTGAACTTGTCTTTCCTTCCAAAATCATTGCTTTCCTCAACAGTTATTTTAAGTGTTTCTTTAATCCATGGTAATTATAGTTCTTTAAAAATTAAAACCCTAAATTTATACAAAGATTTCAAGTTTATGGAGTTTTCTTTTTTTCTTTTTTTTTTCTTTTTTTTTCTTTTTTCTTTTTTTTTTAATTCTTGAATTTACGTAATAGTGCAAATATTGTCTCTTTTGGCTTGTTACTTATTGTCATCCGTCTCGCAGTTTCAAAATGTTTTCTTATAAGGGTATGGAAACTCGTCTCTAACATACGCATTTTAAAACCCTAAGGTTGACACCTATACGTAACTGGCCAAAACAGACAATATCTACTAGCATACGCGTTTTAAAACCCTAAGGCTGACACCTATACGTAACTGGCCAAAACAGACAATATCTACTAGCGGTGAACTTCGACTATTACAAATGATATCAAAGCTAGATAATGTATAATGTGCCAGCAAAGACGTTGGGCCTCAAGAGTGTGGATTGTGAGATCCCATATAGGTTGGAGAGGGAAACGAAACGTTTCTTACAAAAGTGTGGAAACCTCTTGTGTGGTTTAAAACTGAATGAAAGTAAAATTATTTAATTTGTTTATTAAGAGAAATAAAAACAGTGACTTTTTTCAATTTATTATTCCTACTCCGAACAAACTTCCACTGAAATTTCTTTTAGAAAGAAGAAAAATATTAGAACCAGTAAGGAGGTTCCTGCAAAAAAAATTTAAAAATCAAAACTTGGAGAATAAACCTTCCTTACATACAAAGGAGCTTCATTGATCATAACTTGGTGTTCATATAAAGAATGAAACTAAACCAACAAATAAAAAATTTTAATTTTAATTTTCAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACGTACATGAGTGGGGTAATGCATCTACCCAGCGCTGCTTCAGCCAACATCGTTACAGATTTCATGGGCACTTGCTTTCTCCTTTCTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTGGGCCGATACTATACCATTCTCATCTCTGCTTCAATCCAAACACTGGTTTGTTCATCAAACTCACTTAAATCAATCATTTCAAGAACATATAGTGGGTGTTGATTACGATTGTATGCTACTATAGGGAACTGCTGCACTTGCAATTGCAACAAAACTGCCACAGCTTCGTCCGCCGCCGTGTGATGGCACCAACACCTGTAAGGAAGCAAATGGGTTTCAAATGGGAATTCTGTACCTGACTCTGTATCTCATTGCTTTGGGGACGGGTGGACTAAAATCAAGCATTTCGGGCTTTGGAACCGACCAGTTTGATGAGAAAGATGAGAAGGAAAAGACTAAGATGGCCTTTTTCTTTAACAGATTCTTTTTCTTTATCAGCACTGGAACACTCATGGCAGTCACTGTGCTGGTTTACATTCAAGATGAAGTGGGTCGGAGTTGGGCTTATGGGATTTGTTCTGTCTCAATGCTTGTCGCCATTTTGTTGTTCTTGTCGGGGACCAAGCGGTACAGATACAAGAAGAGCATGGGAAGCCCTGTAATTCACATTCTCCAAGTCATTTCTGCTGCAATCAAAAACAGGAAACTGGAGCTTCCTCACAATGTTGCTTTGCTGTATGAGGATTCTTCTGAGCATGCTACAAGAATCCAGCACACAAAACAATTCCAGTAAGTTCATTGATTAGCTGCAATTTTCAAGTGATGTCATTAATTTGTGATTCCTTTTCGTATAGGTTCTTGGACAAGGCTGCAATTGTTGCAGAAGGAGATTCAGCTCAAAATCCATGGAAGCTAAGCTCAGTGACAAGAGTGGAGGAGGTGAAAATGGTGGCAAGACTGCTGCCTGTATGGGCCACAACCATACTCTTCTGGACTATATATGCTCAGATGATCACTTTCTCAGTTGTACAAGCATCCACCATGGAAAGAACAATAGGGTCATTCCAAATCCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCTATACTCATCACACTGGGTGTTTATGACCGTCTTATTATGCCTCTATGGAAGAAATGGAAAGGAAAACAAGGTAAATTAAGATCCAATTACAAAATCCTAATCTGGACTTCAAGGAATCCTGAGTAGCTATGTTGTTATGTGACAGGTTTCACAAACATACAGAGAATGGCCATAGGCCTTGTCCTGTCAATACTTGGAATGGCAGCTGCAGCAGTAGCCGAGAGAAAACGGCTAGCAGTGGCAAAAGCAGTGGGTGACAACACAGCTACTCTGCCTATAACGGTGTTCCTATTGATCCCACAGTTTTTCTTGGTGGGTTCAGGTGAAGCCTTCATATATACAGGGCAGCTTGATTTCTTCATAACCAATTCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTGTTCCTCTCCACTTTATCACTCGGGTTTTTCATCAGCAGTTTCTTGGTTTTGGTTGTGAAGAGGGTGACAGGAAACGATGGACAAGGTTGGCTCGCAGACAATATAAACTACGGCAGACTTGACTGCTTCTATGGGCTATTGACCATATTAAGTGTGATCAACTTTGTTGCTTTTCTGGTTTGTGTTAACCGGTACATTCCGCAAGAAGGCAAAGACTCGCAGAAGAACCACAGCTTGGGTGAAGAGAAATGCTAG

mRNA sequence

TCATTCCTTTCAGAATCTCTGCCTACAAAACCAACAACTCCCTCACCACTTTCCACACTCACTTTCAACCCTTTCAAAAACTAAACCTTCTTTTACCTCATCTTCTCCATAGCTGCTACCTGCAAAGCTTCAAAAATGGATGGGAAGCTAAGTTGTAGAGTGGCTGATGCTGTGGATTACAAAGGCTTTCCTGCTGATAAGTCTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACGTACATGAGTGGGGTAATGCATCTACCCAGCGCTGCTTCAGCCAACATCGTTACAGATTTCATGGGCACTTGCTTTCTCCTTTCTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTGGGCCGATACTATACCATTCTCATCTCTGCTTCAATCCAAACACTGGGAACTGCTGCACTTGCAATTGCAACAAAACTGCCACAGCTTCGTCCGCCGCCGTGTGATGGCACCAACACCTGTAAGGAAGCAAATGGGTTTCAAATGGGAATTCTGTACCTGACTCTGTATCTCATTGCTTTGGGGACGGGTGGACTAAAATCAAGCATTTCGGGCTTTGGAACCGACCAGTTTGATGAGAAAGATGAGAAGGAAAAGACTAAGATGGCCTTTTTCTTTAACAGATTCTTTTTCTTTATCAGCACTGGAACACTCATGGCAGTCACTGTGCTGGTTTACATTCAAGATGAAGTGGGTCGGAGTTGGGCTTATGGGATTTGTTCTGTCTCAATGCTTGTCGCCATTTTGTTGTTCTTGTCGGGGACCAAGCGGTACAGATACAAGAAGAGCATGGGAAGCCCTGTAATTCACATTCTCCAAGTCATTTCTGCTGCAATCAAAAACAGGAAACTGGAGCTTCCTCACAATGTTGCTTTGCTGTATGAGGATTCTTCTGAGCATGCTACAAGAATCCAGCACACAAAACAATTCCAGTTCTTGGACAAGGCTGCAATTGTTGCAGAAGGAGATTCAGCTCAAAATCCATGGAAGCTAAGCTCAGTGACAAGAGTGGAGGAGGTGAAAATGGTGGCAAGACTGCTGCCTGTATGGGCCACAACCATACTCTTCTGGACTATATATGCTCAGATGATCACTTTCTCAGTTGTACAAGCATCCACCATGGAAAGAACAATAGGGTCATTCCAAATCCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCTATACTCATCACACTGGGTGTTTATGACCGTCTTATTATGCCTCTATGGAAGAAATGGAAAGGAAAACAAGGTTTCACAAACATACAGAGAATGGCCATAGGCCTTGTCCTGTCAATACTTGGAATGGCAGCTGCAGCAGTAGCCGAGAGAAAACGGCTAGCAGTGGCAAAAGCAGTGGGTGACAACACAGCTACTCTGCCTATAACGGTGTTCCTATTGATCCCACAGTTTTTCTTGGTGGGTTCAGGTGAAGCCTTCATATATACAGGGCAGCTTGATTTCTTCATAACCAATTCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTGTTCCTCTCCACTTTATCACTCGGGTTTTTCATCAGCAGTTTCTTGGTTTTGGTTGTGAAGAGGGTGACAGGAAACGATGGACAAGGTTGGCTCGCAGACAATATAAACTACGGCAGACTTGACTGCTTCTATGGGCTATTGACCATATTAAGTGTGATCAACTTTGTTGCTTTTCTGGTTTGTGTTAACCGGTACATTCCGCAAGAAGGCAAAGACTCGCAGAAGAACCACAGCTTGGGTGAAGAGAAATGCTAG

Coding sequence (CDS)

ATGGATGGGAAGCTAAGTTGTAGAGTGGCTGATGCTGTGGATTACAAAGGCTTTCCTGCTGATAAGTCTAAGACTGGTGGTTGGATTCCTGCTGCTCTTATTTTAGGTATTGAAATATGTGAAAGGCTGTCAACAATGGGAATAGCAGTGAATCTTGTGACGTACATGAGTGGGGTAATGCATCTACCCAGCGCTGCTTCAGCCAACATCGTTACAGATTTCATGGGCACTTGCTTTCTCCTTTCTTTGCTTGGAGGCTTTCTTGCTGATTCTTTCCTGGGCCGATACTATACCATTCTCATCTCTGCTTCAATCCAAACACTGGGAACTGCTGCACTTGCAATTGCAACAAAACTGCCACAGCTTCGTCCGCCGCCGTGTGATGGCACCAACACCTGTAAGGAAGCAAATGGGTTTCAAATGGGAATTCTGTACCTGACTCTGTATCTCATTGCTTTGGGGACGGGTGGACTAAAATCAAGCATTTCGGGCTTTGGAACCGACCAGTTTGATGAGAAAGATGAGAAGGAAAAGACTAAGATGGCCTTTTTCTTTAACAGATTCTTTTTCTTTATCAGCACTGGAACACTCATGGCAGTCACTGTGCTGGTTTACATTCAAGATGAAGTGGGTCGGAGTTGGGCTTATGGGATTTGTTCTGTCTCAATGCTTGTCGCCATTTTGTTGTTCTTGTCGGGGACCAAGCGGTACAGATACAAGAAGAGCATGGGAAGCCCTGTAATTCACATTCTCCAAGTCATTTCTGCTGCAATCAAAAACAGGAAACTGGAGCTTCCTCACAATGTTGCTTTGCTGTATGAGGATTCTTCTGAGCATGCTACAAGAATCCAGCACACAAAACAATTCCAGTTCTTGGACAAGGCTGCAATTGTTGCAGAAGGAGATTCAGCTCAAAATCCATGGAAGCTAAGCTCAGTGACAAGAGTGGAGGAGGTGAAAATGGTGGCAAGACTGCTGCCTGTATGGGCCACAACCATACTCTTCTGGACTATATATGCTCAGATGATCACTTTCTCAGTTGTACAAGCATCCACCATGGAAAGAACAATAGGGTCATTCCAAATCCCAGCAGGCTCTCTCACTGTCTTCTTTGTAGCAGCTATACTCATCACACTGGGTGTTTATGACCGTCTTATTATGCCTCTATGGAAGAAATGGAAAGGAAAACAAGGTTTCACAAACATACAGAGAATGGCCATAGGCCTTGTCCTGTCAATACTTGGAATGGCAGCTGCAGCAGTAGCCGAGAGAAAACGGCTAGCAGTGGCAAAAGCAGTGGGTGACAACACAGCTACTCTGCCTATAACGGTGTTCCTATTGATCCCACAGTTTTTCTTGGTGGGTTCAGGTGAAGCCTTCATATATACAGGGCAGCTTGATTTCTTCATAACCAATTCTCCCAAAGGGATGAAAACTATGAGTACCGGTCTGTTCCTCTCCACTTTATCACTCGGGTTTTTCATCAGCAGTTTCTTGGTTTTGGTTGTGAAGAGGGTGACAGGAAACGATGGACAAGGTTGGCTCGCAGACAATATAAACTACGGCAGACTTGACTGCTTCTATGGGCTATTGACCATATTAAGTGTGATCAACTTTGTTGCTTTTCTGGTTTGTGTTAACCGGTACATTCCGCAAGAAGGCAAAGACTCGCAGAAGAACCACAGCTTGGGTGAAGAGAAATGCTAG

Protein sequence

MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAEGDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFVAFLVCVNRYIPQEGKDSQKNHSLGEEKC
Homology
BLAST of Carg12161 vs. NCBI nr
Match: KAG7021815.1 (Protein NRT1/ PTR FAMILY 6.2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK
Sbjct: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK
Sbjct: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV
Sbjct: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNHSLGEEKC
Sbjct: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 568

BLAST of Carg12161 vs. NCBI nr
Match: KAG6587927.1 (Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 567/568 (99.82%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 51  MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 110

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP
Sbjct: 111 HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 170

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK
Sbjct: 171 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 230

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK
Sbjct: 231 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 290

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 291 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 350

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GD+AQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 351 GDTAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 410

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 411 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 470

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 471 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 530

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV
Sbjct: 531 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 590

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNHSLGEEKC
Sbjct: 591 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 618

BLAST of Carg12161 vs. NCBI nr
Match: XP_022933906.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita moschata])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 565/568 (99.47%), Postives = 567/568 (99.82%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 51  MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 110

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP
Sbjct: 111 HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 170

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK
Sbjct: 171 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 230

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK
Sbjct: 231 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 290

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 291 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 350

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GD+AQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 351 GDTAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 410

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 411 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 470

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAV DNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 471 VAERKRLAVAKAVDDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 530

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLAD+INYGRLDCFYGLLTILSVINFV
Sbjct: 531 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADDINYGRLDCFYGLLTILSVINFV 590

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNHSLGEEKC
Sbjct: 591 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 618

BLAST of Carg12161 vs. NCBI nr
Match: XP_023529436.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 559/568 (98.42%), Postives = 563/568 (99.12%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGT ALAI TKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTVALAITTKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGT TCKEANGF+MGILYLTLYLIALGTGGLKSSISGFGTDQFDE+DEKEKTK
Sbjct: 121 QLRPPPCDGTKTCKEANGFEMGILYLTLYLIALGTGGLKSSISGFGTDQFDERDEKEKTK 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSM VAILLFLSGTKRYRYK
Sbjct: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMFVAILLFLSGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMER+IGSF
Sbjct: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERSIGSF 360

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAVGDNT TLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 421 VAERKRLAVAKAVGDNTTTLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV
Sbjct: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNH+LGEEKC
Sbjct: 541 AFLVCVNRYIPQEGKDSQKNHNLGEEKC 568

BLAST of Carg12161 vs. NCBI nr
Match: XP_023000948.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita maxima])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 560/568 (98.59%), Postives = 562/568 (98.94%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAI TKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGT TCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKE TK
Sbjct: 121 QLRPPPCDGTKTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKETTK 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSM VAILLFLSGTKRYRYK
Sbjct: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMFVAILLFLSGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAVGDNTATLP+TVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 421 VAERKRLAVAKAVGDNTATLPVTVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTI SVINFV
Sbjct: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTISSVINFV 540

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNH+L EEKC
Sbjct: 541 AFLVCVNRYIPQEGKDSQKNHNLAEEKC 568

BLAST of Carg12161 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 4.9e-230
Identity = 418/566 (73.85%), Postives = 471/566 (83.22%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           M+ K S  VADAVDYKG PADKSKTGGWI AALILGIE+ ERLSTMGIAVNLVTY+   M
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPS+ SANIVTDFMGT FLL LLGGFLADSFLGR+ TI I ++IQ LGT ALA+ATKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           +LRPP C     C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFD+KD KEK  
Sbjct: 121 ELRPPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAH 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAYGIC+VSM +AI++FL GTKRYRYK
Sbjct: 181 MAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KS GSPV+ I QVI+AA + RK+ELP ++  LYED+ E   RI+HT QF  LDKAAIVAE
Sbjct: 241 KSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPE-GIRIEHTDQFHLLDKAAIVAE 300

Query: 301 GDSAQ--------NPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQAST 360
           GD  Q        NPWKLSSVT+VEEVKM+ RLLP+WATTI+FWT YAQMITFSV QAST
Sbjct: 301 GDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           M R IGSF+IPAGSLTVFFVAAILITL VYDR IMP WKKWKGK GF+++QR+AIGLVLS
Sbjct: 361 MRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLS 420

Query: 421 ILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITN 480
             GMAAAA+ E+KRL+VAK+   +  TLPI+VFLL+PQFFLVG+GEAFIYTGQLDFFIT 
Sbjct: 421 TAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQ 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLL 540
           SPKGMKTMSTGLFL+TLSLGFF+SSFLV +VKRVT      GWLADNIN+GRLD FY LL
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLL 540

Query: 541 TILSVINFVAFLVCVNRYIPQEGKDS 558
            ILS INFV +++C   + P +GKDS
Sbjct: 541 VILSGINFVVYIICALWFKPTKGKDS 563

BLAST of Carg12161 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 2.0e-162
Identity = 300/571 (52.54%), Postives = 408/571 (71.45%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANI 70
           DA D++G PAD+SKTGGW  AA+IL IE  ERL+T+GI VNLVTY++G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 71  VTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGT 130
           VT+F+GT F+L LLGGF+AD+FLGRY TI I A+IQ  G + L ++T +P LRPP C+ T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 131 NT--CKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRF 190
            +  C++A+G Q+ +LYL LYL ALGTGG+K+S+SGFG+DQFDE + KE++KM +FFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 191 FFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVI 250
           FF I+ G+L+AVTVLVY+QD+VGR W YGIC+ ++++A+ +FL+GT RYR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 251 HILQVISAAIKNRKLELPHNVALLY--------EDSSEHATRIQHTKQFQFLDKAAI--- 310
            +  VI AA +NRKLELP + + LY        E S +   ++ HT+QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQ 313

Query: 311 -VAEGDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERT 370
                 +  N W LS++T VEEVK + R+LP+WAT ILFWT++AQ+ T SV Q+ T++R+
Sbjct: 314 EAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRS 373

Query: 371 IGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILG 430
           IGSF+IP  S+ VF+V  +L+T  VYDR+ + L KK +    G   +QR+ +GL    + 
Sbjct: 374 IGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMA 433

Query: 431 MAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPK 490
           MA AA+ E KRL  A A G    TLP+  +LLIPQ+ +VG GEA IYTGQLDFF+   PK
Sbjct: 434 MAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPK 493

Query: 491 GMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILS 550
           GMK MSTGL LSTL+LGFF SS LV +V++ TG     W+AD++N GRL  FY L+ +L 
Sbjct: 494 GMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVAVLV 553

Query: 551 VINFVAFLVCVNRYIPQEGKDSQKNHSLGEE 567
            +NF+ FLV    Y+ +E + ++    L +E
Sbjct: 554 ALNFLIFLVFSKWYVYKEKRLAEVGIELDDE 583

BLAST of Carg12161 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.7e-145
Identity = 282/546 (51.65%), Postives = 375/546 (68.68%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANI 70
           +A DY+G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTY+ G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDG- 130
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISAS+  LG   L +AT +  +RPP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 ---TNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFN 190
               + C EANG Q+ +LY+ LY IALG GG+KS++SGFG+DQFD  D KE+ +M FFFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSP 250
           RF+F IS G+L AV  LVY+QD VGR W YGI + +M+VA ++ L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 VIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAEGDS--- 310
              I +V   A K RK   P + +LL   +    T + HT+  + LDKAAI     S   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---NGYDNTTVPHTEMLKCLDKAAISKNESSPSS 315

Query: 311 ----AQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGS 370
                ++PW +S+VT+VEEVK+V +L+P+WAT ILFWTIY+QM TF+V QA+ M+R +GS
Sbjct: 316 KDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGS 375

Query: 371 FQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILGMAA 430
           F +PAGS + F +  IL+   + +R+ +PL ++  K  QG T++QR+ +GLV S+  MA 
Sbjct: 376 FTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAV 435

Query: 431 AAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMK 490
           AAV E  R    +A  +N     I+ F L+PQ+FLVG+GEAF Y GQL+FFI  +P+ MK
Sbjct: 436 AAVIENAR---REAAVNNDK--KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMK 495

Query: 491 TMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVIN 545
           +MSTGLFLST+S+GFF+SS LV +V RVT    + WL  N+N  RL+ FY LL +L  +N
Sbjct: 496 SMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGALN 550

BLAST of Carg12161 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 6.6e-118
Identity = 231/550 (42.00%), Postives = 352/550 (64.00%), Query Frame = 0

Query: 13  VDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANIVT 72
           VD    PA+K KTG W     ILG E CERL+  G+  NLV Y+   ++  +A +AN VT
Sbjct: 13  VDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVT 72

Query: 73  DFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGTNT 132
           ++ GTC++  L+G F+AD++LGRY+TI     I   G   L ++  +P L+P  C+  +T
Sbjct: 73  NWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADT 132

Query: 133 CKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRFFFFI 192
           C   N  Q  + ++ LY+IALGTGG+K  +S FG DQFDE DE EK K + FFN F+F I
Sbjct: 133 C-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSI 192

Query: 193 STGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVIHILQ 252
           + G L+A TVLV+IQ  VG  W +G+ +V+M++A+  F  G++ YR ++  GSP+  I Q
Sbjct: 193 NVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQ 252

Query: 253 VISAAIKNRKLELPHNVALLYEDSSEHAT-----RIQHTKQFQFLDKAAIVAEGDSAQ-- 312
           VI AA +   +++P + +LL+E + + +      ++ HT   +F DKAA+ ++ DS +  
Sbjct: 253 VIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDG 312

Query: 313 --NPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIG-SFQI 372
             NPW+L SVT+VEE+K +  LLPVWAT I+F T+Y+QM T  V+Q +TM++ +G +F+I
Sbjct: 313 EVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEI 372

Query: 373 PAGSLTVFFVAAILITLGVYDRLIMPLWKKW-KGKQGFTNIQRMAIGLVLSILGMAAAAV 432
           P+ SL++F   ++L    VYD+ I+PL +K+ + ++GFT +QRM IGLV+SI  M  A V
Sbjct: 373 PSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGV 432

Query: 433 AERKRLAVAKAVGD-NTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 492
            E  RL   K     +   + +++F  IPQ+ L+G  E F + GQL+FF   +P  M+++
Sbjct: 433 LEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSL 492

Query: 493 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLLTILSVINF 550
            + L L+T++LG ++S+ LV VV ++T  +G+ GW+ DN+N G LD F+ LL  LS +NF
Sbjct: 493 CSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNF 552

BLAST of Carg12161 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 9.5e-117
Identity = 232/551 (42.11%), Postives = 350/551 (63.52%), Query Frame = 0

Query: 12  AVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANIV 71
           +VD+ G P  K KTG W     ILG E CERL+  GIA NL+TY++  +H  + ++A  V
Sbjct: 29  SVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 88

Query: 72  TDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGTN 131
           T + GTC+L  L+G  LAD++ GRY+TI   + I  +G +AL ++  +P L+P  C G +
Sbjct: 89  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIG-D 148

Query: 132 TCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRFFFF 191
            C  A   Q  + +  LYLIALGTGG+K  +S FG DQFD+ D +E+ + A FFN F+F 
Sbjct: 149 FCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFS 208

Query: 192 ISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVIHIL 251
           I+ G L++ ++LV+IQ+  G    +GI +V M +AI  F  GT  YR++K  GSP+  I 
Sbjct: 209 INIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRIS 268

Query: 252 QVISAAIKNRKLELPHNVALLYEDSSEHAT-----RIQHTKQFQFLDKAAIVAEGDSA-- 311
           QV+ A+ +   +++P +  LLYE   +++      +I+HT   Q+LDKAA+++E +S   
Sbjct: 269 QVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEESKSG 328

Query: 312 --QNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSFQI 371
              N W+L +VT+VEE+K++ R+ P+WA+ I+F  +YAQM T  V Q   M   IGSFQ+
Sbjct: 329 DYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQL 388

Query: 372 PAGSLTVFFVAAILITLGVYDRLIMPLWKKWKG-KQGFTNIQRMAIGLVLSILGMAAAAV 431
           P  +L  F  A+++I + +YDR I+PL +K+ G  +GFT IQRM IGL +S+L MAAAA+
Sbjct: 389 PPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAI 448

Query: 432 AERKRLAVAKAVG--DNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKT 491
            E  RL +A  +G  ++ A +PI+V   IPQ+F++G+ E F + GQL+FF   SP  M++
Sbjct: 449 VEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRS 508

Query: 492 MSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLLTILSVIN 550
           + + L L T +LG ++SS ++ +V   T  +GQ GW++DN+N G LD F+ LL  LS++N
Sbjct: 509 LCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGLSLVN 568

BLAST of Carg12161 vs. ExPASy TrEMBL
Match: A0A6J1F0B4 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita moschata OX=3662 GN=LOC111441177 PE=3 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 565/568 (99.47%), Postives = 567/568 (99.82%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 51  MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 110

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP
Sbjct: 111 HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 170

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK
Sbjct: 171 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 230

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK
Sbjct: 231 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 290

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 291 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 350

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GD+AQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 351 GDTAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 410

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 411 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 470

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAV DNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 471 VAERKRLAVAKAVDDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 530

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLAD+INYGRLDCFYGLLTILSVINFV
Sbjct: 531 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADDINYGRLDCFYGLLTILSVINFV 590

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNHSLGEEKC
Sbjct: 591 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 618

BLAST of Carg12161 vs. ExPASy TrEMBL
Match: A0A6J1KP37 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita maxima OX=3661 GN=LOC111495229 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 560/568 (98.59%), Postives = 562/568 (98.94%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM
Sbjct: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAI TKLP
Sbjct: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAITTKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           QLRPPPCDGT TCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKE TK
Sbjct: 121 QLRPPPCDGTKTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKETTK 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSM VAILLFLSGTKRYRYK
Sbjct: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMFVAILLFLSGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE
Sbjct: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300

Query: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360
           GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF
Sbjct: 301 GDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSF 360

Query: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420
           QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA
Sbjct: 361 QIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLSILGMAAAA 420

Query: 421 VAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480
           VAERKRLAVAKAVGDNTATLP+TVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM
Sbjct: 421 VAERKRLAVAKAVGDNTATLPVTVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 480

Query: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVINFV 540
           STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTI SVINFV
Sbjct: 481 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTISSVINFV 540

Query: 541 AFLVCVNRYIPQEGKDSQKNHSLGEEKC 569
           AFLVCVNRYIPQEGKDSQKNH+L EEKC
Sbjct: 541 AFLVCVNRYIPQEGKDSQKNHNLAEEKC 568

BLAST of Carg12161 vs. ExPASy TrEMBL
Match: A0A5A7TZJ0 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00850 PE=3 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.0e-286
Identity = 514/580 (88.62%), Postives = 541/580 (93.28%), Query Frame = 0

Query: 1   MDG-KLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGV 60
           MDG KLS  VADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTY+ GV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKL 120
           MHLPSAASANIVTDFMGTCFLL LLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKT 180
           PQLRPPPCDGT TCKEANGFQMGILYL LYLIALGTGGLKSSISGFGTDQFD+ DEKEK 
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRY 240
           KMA+FFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML+AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVA 300
           KKSMGSPV+ ILQVI+AAIK RKLELP NV LLYEDSS+HA+RI+HTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGD-------SAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQAST 360
           EGD       SA NPWKLSSVT+VEEVKM+ARLLP+WATTI+FWT YAQMITFSV QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITN 480
           ILGMAAAA+AERKRLAVAKAVG +T TLPI+VFLLIPQFFLVG+GEAFIYTGQLDFFIT 
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLV VVKRVTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSVINFVAFLVCVNRYIPQEGKDSQK----NHSLGEEKC 569
           ILS+INFVAFLVCV  Y+PQ+ KD QK    N +L EEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Carg12161 vs. ExPASy TrEMBL
Match: A0A1S3B9G1 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo OX=3656 GN=LOC103487457 PE=3 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.0e-286
Identity = 514/580 (88.62%), Postives = 541/580 (93.28%), Query Frame = 0

Query: 1   MDG-KLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGV 60
           MDG KLS  VADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTY+ GV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKL 120
           MHLPSAASANIVTDFMGTCFLL LLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKT 180
           PQLRPPPCDGT TCKEANGFQMGILYL LYLIALGTGGLKSSISGFGTDQFD+ DEKEK 
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRY 240
           KMA+FFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML+AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVA 300
           KKSMGSPV+ ILQVI+AAIK RKLELP NV LLYEDSS+HA+RI+HTKQFQFLDKAAIVA
Sbjct: 241 KKSMGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGD-------SAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQAST 360
           EGD       SA NPWKLSSVT+VEEVKM+ARLLP+WATTI+FWT YAQMITFSV QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITN 480
           ILGMAAAA+AERKRLAVAKAVG +T TLPI+VFLLIPQFFLVG+GEAFIYTGQLDFFIT 
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLV VVKRVTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSVINFVAFLVCVNRYIPQEGKDSQK----NHSLGEEKC 569
           ILS+INFVAFLVCV  Y+PQ+ KD QK    N +L EEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Carg12161 vs. ExPASy TrEMBL
Match: A0A5D3DTZ7 (Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00110 PE=3 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.5e-285
Identity = 513/580 (88.45%), Postives = 541/580 (93.28%), Query Frame = 0

Query: 1   MDG-KLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGV 60
           MDG KLS  VADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTY+ GV
Sbjct: 1   MDGKKLSWTVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYLGGV 60

Query: 61  MHLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKL 120
           MHLPSAASANIVTDFMGTCFLL LLGGFLADSFLGRYYTILISA IQTLGTA LA++TKL
Sbjct: 61  MHLPSAASANIVTDFMGTCFLLCLLGGFLADSFLGRYYTILISAIIQTLGTATLAVSTKL 120

Query: 121 PQLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKT 180
           PQLRPPPCDGT TCKEANGFQMGILYL LYLIALGTGGLKSSISGFGTDQFD+ DEKEK 
Sbjct: 121 PQLRPPPCDGTKTCKEANGFQMGILYLALYLIALGTGGLKSSISGFGTDQFDDTDEKEKA 180

Query: 181 KMAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRY 240
           KMA+FFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSML+AILLF+SGTKRYRY
Sbjct: 181 KMAYFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLIAILLFVSGTKRYRY 240

Query: 241 KKSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVA 300
           KKS+GSPV+ ILQVI+AAIK RKLELP NV LLYEDSS+HA+RI+HTKQFQFLDKAAIVA
Sbjct: 241 KKSIGSPVVQILQVIAAAIKKRKLELPQNVGLLYEDSSDHASRIEHTKQFQFLDKAAIVA 300

Query: 301 EGD-------SAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQAST 360
           EGD       SA NPWKLSSVT+VEEVKM+ARLLP+WATTI+FWT YAQMITFSV QAST
Sbjct: 301 EGDFQAHGAVSALNPWKLSSVTKVEEVKMMARLLPIWATTIIFWTTYAQMITFSVQQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           MER+IGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS
Sbjct: 361 MERSIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420

Query: 421 ILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITN 480
           ILGMAAAA+AERKRLAVAKAVG +T TLPI+VFLLIPQFFLVG+GEAFIYTGQLDFFIT 
Sbjct: 421 ILGMAAAALAERKRLAVAKAVGGDTTTLPISVFLLIPQFFLVGAGEAFIYTGQLDFFITK 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLT 540
           SPKGMKTMSTGLFL+TLSLGFFISSFLV VVKRVTGNDG+GWLADNINYGRLDCFYGLLT
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFISSFLVSVVKRVTGNDGEGWLADNINYGRLDCFYGLLT 540

Query: 541 ILSVINFVAFLVCVNRYIPQEGKDSQK----NHSLGEEKC 569
           ILS+INFVAFLVCV  Y+PQ+ KD QK    N +L EEKC
Sbjct: 541 ILSIINFVAFLVCVIWYMPQKRKDFQKNGIVNGNLAEEKC 580

BLAST of Carg12161 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 798.5 bits (2061), Expect = 3.5e-231
Identity = 418/566 (73.85%), Postives = 471/566 (83.22%), Query Frame = 0

Query: 1   MDGKLSCRVADAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVM 60
           M+ K S  VADAVDYKG PADKSKTGGWI AALILGIE+ ERLSTMGIAVNLVTY+   M
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 61  HLPSAASANIVTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLP 120
           HLPS+ SANIVTDFMGT FLL LLGGFLADSFLGR+ TI I ++IQ LGT ALA+ATKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 121 QLRPPPCDGTNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTK 180
           +LRPP C     C  A  FQM ILY++LYLIALGTGGLKSSISGFG+DQFD+KD KEK  
Sbjct: 121 ELRPPTCHHGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAH 180

Query: 181 MAFFFNRFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYK 240
           MAFFFNRFFFFIS GTL+AVTVLVY+QDEVGRSWAYGIC+VSM +AI++FL GTKRYRYK
Sbjct: 181 MAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYK 240

Query: 241 KSMGSPVIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAE 300
           KS GSPV+ I QVI+AA + RK+ELP ++  LYED+ E   RI+HT QF  LDKAAIVAE
Sbjct: 241 KSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPE-GIRIEHTDQFHLLDKAAIVAE 300

Query: 301 GDSAQ--------NPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQAST 360
           GD  Q        NPWKLSSVT+VEEVKM+ RLLP+WATTI+FWT YAQMITFSV QAST
Sbjct: 301 GDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAST 360

Query: 361 MERTIGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKKWKGKQGFTNIQRMAIGLVLS 420
           M R IGSF+IPAGSLTVFFVAAILITL VYDR IMP WKKWKGK GF+++QR+AIGLVLS
Sbjct: 361 MRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLS 420

Query: 421 ILGMAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITN 480
             GMAAAA+ E+KRL+VAK+   +  TLPI+VFLL+PQFFLVG+GEAFIYTGQLDFFIT 
Sbjct: 421 TAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQ 480

Query: 481 SPKGMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLL 540
           SPKGMKTMSTGLFL+TLSLGFF+SSFLV +VKRVT      GWLADNIN+GRLD FY LL
Sbjct: 481 SPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLL 540

Query: 541 TILSVINFVAFLVCVNRYIPQEGKDS 558
            ILS INFV +++C   + P +GKDS
Sbjct: 541 VILSGINFVVYIICALWFKPTKGKDS 563

BLAST of Carg12161 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 573.9 bits (1478), Expect = 1.4e-163
Identity = 300/571 (52.54%), Postives = 408/571 (71.45%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANI 70
           DA D++G PAD+SKTGGW  AA+IL IE  ERL+T+GI VNLVTY++G MHL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 71  VTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGT 130
           VT+F+GT F+L LLGGF+AD+FLGRY TI I A+IQ  G + L ++T +P LRPP C+ T
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 131 NT--CKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRF 190
            +  C++A+G Q+ +LYL LYL ALGTGG+K+S+SGFG+DQFDE + KE++KM +FFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 191 FFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVI 250
           FF I+ G+L+AVTVLVY+QD+VGR W YGIC+ ++++A+ +FL+GT RYR+KK +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 251 HILQVISAAIKNRKLELPHNVALLY--------EDSSEHATRIQHTKQFQFLDKAAI--- 310
            +  VI AA +NRKLELP + + LY        E S +   ++ HT+QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQ 313

Query: 311 -VAEGDSAQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERT 370
                 +  N W LS++T VEEVK + R+LP+WAT ILFWT++AQ+ T SV Q+ T++R+
Sbjct: 314 EAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRS 373

Query: 371 IGSFQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILG 430
           IGSF+IP  S+ VF+V  +L+T  VYDR+ + L KK +    G   +QR+ +GL    + 
Sbjct: 374 IGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMA 433

Query: 431 MAAAAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPK 490
           MA AA+ E KRL  A A G    TLP+  +LLIPQ+ +VG GEA IYTGQLDFF+   PK
Sbjct: 434 MAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPK 493

Query: 491 GMKTMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILS 550
           GMK MSTGL LSTL+LGFF SS LV +V++ TG     W+AD++N GRL  FY L+ +L 
Sbjct: 494 GMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK-AHPWIADDLNKGRLYNFYWLVAVLV 553

Query: 551 VINFVAFLVCVNRYIPQEGKDSQKNHSLGEE 567
            +NF+ FLV    Y+ +E + ++    L +E
Sbjct: 554 ALNFLIFLVFSKWYVYKEKRLAEVGIELDDE 583

BLAST of Carg12161 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.2e-146
Identity = 282/546 (51.65%), Postives = 375/546 (68.68%), Query Frame = 0

Query: 11  DAVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANI 70
           +A DY+G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTY+ G +H+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 71  VTDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDG- 130
           VT+FMGT  LL LLGGFLAD+ LGRY  + ISAS+  LG   L +AT +  +RPP CD  
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 131 ---TNTCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFN 190
               + C EANG Q+ +LY+ LY IALG GG+KS++SGFG+DQFD  D KE+ +M FFFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 191 RFFFFISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSP 250
           RF+F IS G+L AV  LVY+QD VGR W YGI + +M+VA ++ L GTKRYR+KK  GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 251 VIHILQVISAAIKNRKLELPHNVALLYEDSSEHATRIQHTKQFQFLDKAAIVAEGDS--- 310
              I +V   A K RK   P + +LL   +    T + HT+  + LDKAAI     S   
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL---NGYDNTTVPHTEMLKCLDKAAISKNESSPSS 315

Query: 311 ----AQNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGS 370
                ++PW +S+VT+VEEVK+V +L+P+WAT ILFWTIY+QM TF+V QA+ M+R +GS
Sbjct: 316 KDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGS 375

Query: 371 FQIPAGSLTVFFVAAILITLGVYDRLIMPLWKK-WKGKQGFTNIQRMAIGLVLSILGMAA 430
           F +PAGS + F +  IL+   + +R+ +PL ++  K  QG T++QR+ +GLV S+  MA 
Sbjct: 376 FTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAV 435

Query: 431 AAVAERKRLAVAKAVGDNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMK 490
           AAV E  R    +A  +N     I+ F L+PQ+FLVG+GEAF Y GQL+FFI  +P+ MK
Sbjct: 436 AAVIENAR---REAAVNNDK--KISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMK 495

Query: 491 TMSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQGWLADNINYGRLDCFYGLLTILSVIN 545
           +MSTGLFLST+S+GFF+SS LV +V RVT    + WL  N+N  RL+ FY LL +L  +N
Sbjct: 496 SMSTGLFLSTISMGFFVSSLLVSLVDRVT---DKSWLRSNLNKARLNYFYWLLVVLGALN 550

BLAST of Carg12161 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 426.0 bits (1094), Expect = 4.7e-119
Identity = 231/550 (42.00%), Postives = 352/550 (64.00%), Query Frame = 0

Query: 13  VDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANIVT 72
           VD    PA+K KTG W     ILG E CERL+  G+  NLV Y+   ++  +A +AN VT
Sbjct: 13  VDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVT 72

Query: 73  DFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGTNT 132
           ++ GTC++  L+G F+AD++LGRY+TI     I   G   L ++  +P L+P  C+  +T
Sbjct: 73  NWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADT 132

Query: 133 CKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRFFFFI 192
           C   N  Q  + ++ LY+IALGTGG+K  +S FG DQFDE DE EK K + FFN F+F I
Sbjct: 133 C-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSI 192

Query: 193 STGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVIHILQ 252
           + G L+A TVLV+IQ  VG  W +G+ +V+M++A+  F  G++ YR ++  GSP+  I Q
Sbjct: 193 NVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQ 252

Query: 253 VISAAIKNRKLELPHNVALLYEDSSEHAT-----RIQHTKQFQFLDKAAIVAEGDSAQ-- 312
           VI AA +   +++P + +LL+E + + +      ++ HT   +F DKAA+ ++ DS +  
Sbjct: 253 VIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDG 312

Query: 313 --NPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIG-SFQI 372
             NPW+L SVT+VEE+K +  LLPVWAT I+F T+Y+QM T  V+Q +TM++ +G +F+I
Sbjct: 313 EVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEI 372

Query: 373 PAGSLTVFFVAAILITLGVYDRLIMPLWKKW-KGKQGFTNIQRMAIGLVLSILGMAAAAV 432
           P+ SL++F   ++L    VYD+ I+PL +K+ + ++GFT +QRM IGLV+SI  M  A V
Sbjct: 373 PSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGV 432

Query: 433 AERKRLAVAKAVGD-NTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKTM 492
            E  RL   K     +   + +++F  IPQ+ L+G  E F + GQL+FF   +P  M+++
Sbjct: 433 LEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSL 492

Query: 493 STGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLLTILSVINF 550
            + L L+T++LG ++S+ LV VV ++T  +G+ GW+ DN+N G LD F+ LL  LS +NF
Sbjct: 493 CSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNF 552

BLAST of Carg12161 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 422.2 bits (1084), Expect = 6.8e-118
Identity = 232/551 (42.11%), Postives = 350/551 (63.52%), Query Frame = 0

Query: 12  AVDYKGFPADKSKTGGWIPAALILGIEICERLSTMGIAVNLVTYMSGVMHLPSAASANIV 71
           +VD+ G P  K KTG W     ILG E CERL+  GIA NL+TY++  +H  + ++A  V
Sbjct: 29  SVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 88

Query: 72  TDFMGTCFLLSLLGGFLADSFLGRYYTILISASIQTLGTAALAIATKLPQLRPPPCDGTN 131
           T + GTC+L  L+G  LAD++ GRY+TI   + I  +G +AL ++  +P L+P  C G +
Sbjct: 89  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIG-D 148

Query: 132 TCKEANGFQMGILYLTLYLIALGTGGLKSSISGFGTDQFDEKDEKEKTKMAFFFNRFFFF 191
            C  A   Q  + +  LYLIALGTGG+K  +S FG DQFD+ D +E+ + A FFN F+F 
Sbjct: 149 FCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFS 208

Query: 192 ISTGTLMAVTVLVYIQDEVGRSWAYGICSVSMLVAILLFLSGTKRYRYKKSMGSPVIHIL 251
           I+ G L++ ++LV+IQ+  G    +GI +V M +AI  F  GT  YR++K  GSP+  I 
Sbjct: 209 INIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRIS 268

Query: 252 QVISAAIKNRKLELPHNVALLYEDSSEHAT-----RIQHTKQFQFLDKAAIVAEGDSA-- 311
           QV+ A+ +   +++P +  LLYE   +++      +I+HT   Q+LDKAA+++E +S   
Sbjct: 269 QVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEESKSG 328

Query: 312 --QNPWKLSSVTRVEEVKMVARLLPVWATTILFWTIYAQMITFSVVQASTMERTIGSFQI 371
              N W+L +VT+VEE+K++ R+ P+WA+ I+F  +YAQM T  V Q   M   IGSFQ+
Sbjct: 329 DYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQL 388

Query: 372 PAGSLTVFFVAAILITLGVYDRLIMPLWKKWKG-KQGFTNIQRMAIGLVLSILGMAAAAV 431
           P  +L  F  A+++I + +YDR I+PL +K+ G  +GFT IQRM IGL +S+L MAAAA+
Sbjct: 389 PPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAI 448

Query: 432 AERKRLAVAKAVG--DNTATLPITVFLLIPQFFLVGSGEAFIYTGQLDFFITNSPKGMKT 491
            E  RL +A  +G  ++ A +PI+V   IPQ+F++G+ E F + GQL+FF   SP  M++
Sbjct: 449 VEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRS 508

Query: 492 MSTGLFLSTLSLGFFISSFLVLVVKRVTGNDGQ-GWLADNINYGRLDCFYGLLTILSVIN 550
           + + L L T +LG ++SS ++ +V   T  +GQ GW++DN+N G LD F+ LL  LS++N
Sbjct: 509 LCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGLSLVN 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021815.10.0e+00100.00Protein NRT1/ PTR FAMILY 6.2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6587927.10.0e+0099.82Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933906.10.0e+0099.47protein NRT1/ PTR FAMILY 6.2-like [Cucurbita moschata][more]
XP_023529436.10.0e+0098.42protein NRT1/ PTR FAMILY 6.2-like [Cucurbita pepo subsp. pepo][more]
XP_023000948.10.0e+0098.59protein NRT1/ PTR FAMILY 6.2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SZY44.9e-23073.85Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q050852.0e-16252.54Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9LVE01.7e-14551.65Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q9M3906.6e-11842.00Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460329.5e-11742.11Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F0B40.0e+0099.47protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita moschata OX=3662 GN=LOC111441177 ... [more]
A0A6J1KP370.0e+0098.59protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita maxima OX=3661 GN=LOC111495229 PE... [more]
A0A5A7TZJ05.0e-28688.62Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3B9G15.0e-28688.62protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo OX=3656 GN=LOC103487457 PE=3 S... [more]
A0A5D3DTZ71.5e-28588.45Protein NRT1/ PTR FAMILY 6.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT2G26690.13.5e-23173.85Major facilitator superfamily protein [more]
AT1G12110.11.4e-16352.54nitrate transporter 1.1 [more]
AT3G21670.11.2e-14651.65Major facilitator superfamily protein [more]
AT3G54140.14.7e-11942.00peptide transporter 1 [more]
AT2G02040.16.8e-11842.11peptide transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..514
e-value: 2.7E-106
score: 355.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 8..557
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 3..558
e-value: 1.5E-184
score: 616.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 22..550
NoneNo IPR availablePANTHERPTHR11654:SF85PROTEIN NRT1/ PTR FAMILY 6.2coord: 8..557
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 85..109

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12161-RACarg12161-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity