Carg12111 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12111
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Carg_Chr11: 2258618 .. 2259208 (+)
RNA-Seq Expression	Carg12111
Synteny	Carg12111

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTCCTCAAAAATTTATTCCCTCTTCCTAGTTTGCATGCTTTTCCTCTCCACCGCCACTCCCATTTTGGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGGCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCATCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCCTCGGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTAGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAATGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCCGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTCATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

mRNA sequence

ATGGAGTCCTCAAAAATTTATTCCCTCTTCCTAGTTTGCATGCTTTTCCTCTCCACCGCCACTCCCATTTTGGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGGCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCATCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCCTCGGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTAGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAATGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCCGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTCATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

Coding sequence (CDS)

ATGGAGTCCTCAAAAATTTATTCCCTCTTCCTAGTTTGCATGCTTTTCCTCTCCACCGCCACTCCCATTTTGGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGGCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCATCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCCTCGGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTAGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAATGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCCGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTCATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

Protein sequence

MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTCSL

Homology

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BLAST of Carg12111 vs. NCBI nr
Match: KAG7021766.1 (pEARLI1-like lipid transfer protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.2 bits (975), Expect = 1.1e-101
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPP 60
           MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPP
Sbjct: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPP 60

Query: 61  VVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKL 120
           VVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKL
Sbjct: 61  VVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKL 120

Query: 121 GACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQL 180
           GACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQL
Sbjct: 121 GACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQL 180

Query: 181 LISCGKTPPPGYTCSL 197
           LISCGKTPPPGYTCSL
Sbjct: 181 LISCGKTPPPGYTCSL 196

[Close]

BLAST of Carg12111 vs. NCBI nr
Match: XP_023529211.1 (36.4 kDa proline-rich protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 194/206 (94.17%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL------- 60
           MESSKIYSLFL+CMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL       
Sbjct: 1   MESSKIYSLFLLCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPITLPP 60

Query: 61  ---PPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120
              PPIDLPPVVKPPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGK
Sbjct: 61  IVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120

Query: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180
           ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL
Sbjct: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180

Query: 181 NIYVPIALQLLISCGKTPPPGYTCSL 197
           NIYVPIALQLLISCGKTPPPGYTCSL
Sbjct: 181 NIYVPIALQLLISCGKTPPPGYTCSL 206

[Close]

BLAST of Carg12111 vs. NCBI nr
Match: XP_022933855.1 (36.4 kDa proline-rich protein-like [Cucurbita moschata])

HSP 1 Score: 369.8 bits (948), Expect = 1.5e-98
Identity = 194/206 (94.17%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL------- 60
           MESSKIYSLFLVCMLFLSTATPI GCGACGNPPPKKGKPGSGSKSPKGGPIGL       
Sbjct: 1   MESSKIYSLFLVCMLFLSTATPIFGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPITLPP 60

Query: 61  ---PPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120
              PPIDLPPVVKPPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGK
Sbjct: 61  IVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120

Query: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180
           ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL
Sbjct: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180

Query: 181 NIYVPIALQLLISCGKTPPPGYTCSL 197
           NIYVPIALQLLISCGKTPPPGYTCSL
Sbjct: 181 NIYVPIALQLLISCGKTPPPGYTCSL 206

[Close]

BLAST of Carg12111 vs. NCBI nr
Match: XP_022966511.1 (36.4 kDa proline-rich protein-like [Cucurbita maxima])

HSP 1 Score: 365.2 bits (936), Expect = 3.6e-97
Identity = 192/206 (93.20%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL------- 60
           MESSKIYSLFL+CMLFLSTATPI GCGACGNPPPKK KPGSGSKSPKGGPIGL       
Sbjct: 1   MESSKIYSLFLLCMLFLSTATPIFGCGACGNPPPKKVKPGSGSKSPKGGPIGLPPISLPP 60

Query: 61  ---PPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120
              PPIDLPPVVKPPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGK
Sbjct: 61  IVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120

Query: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180
           ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL
Sbjct: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180

Query: 181 NIYVPIALQLLISCGKTPPPGYTCSL 197
           NIYVPIALQLLISCGKTPPPGYTCSL
Sbjct: 181 NIYVPIALQLLISCGKTPPPGYTCSL 206

[Close]

BLAST of Carg12111 vs. NCBI nr
Match: KAG6587881.1 (pEARLI1-like lipid transfer protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 347.4 bits (890), Expect = 7.9e-92
Identity = 182/193 (94.30%), Postives = 183/193 (94.82%), Query Frame = 0


Query: 14  MLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL----------PPIDLPPVVK 73
           MLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL          PPIDLPPVVK
Sbjct: 1   MLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPITLPPIVKPPIDLPPVVK 60

Query: 74  PPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGAC 133
           PPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGAC
Sbjct: 61  PPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGAC 120

Query: 134 VDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS 193
           VDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS
Sbjct: 121 VDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS 180

Query: 194 CGKTPPPGYTCSL 197
           CGKTPPPGYTCSL
Sbjct: 181 CGKTPPPGYTCSL 193

[Close]

BLAST of Carg12111 vs. ExPASy Swiss-Prot
Match: Q00451 (36.4 kDa proline-rich protein OS=Solanum lycopersicum OX=4081 GN=TPRP-F1 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.8e-31
Identity = 91/178 (51.12%), Postives = 113/178 (63.48%), Query Frame = 0


Query: 33  PPKKGKPGSGSKSPKGGPIGLPPI-DLPPVVKPP-ISLPPIVKPP-VDLPPI----PPVT 92
           PP   KP S +      PI  PPI   PPVV PP I  PPIV PP V  PP+    P V 
Sbjct: 166 PPVTPKPPSPTPPIVSPPIVYPPITPTPPVVSPPIIPTPPIVSPPFVPNPPVVIPPPYVP 225

Query: 93  LPPVTVPPVL-TKPSPASKPCP-------PSGKETCPIDTLKLGACVDLLGGLVHIGLGD 152
            PPV  PP++ T P+P   P P       P  + TCPID LKLGACVD+LGGL+HIG+G 
Sbjct: 226 SPPVVTPPIVPTPPTPCPPPPPPPAIIPSPPAQPTCPIDALKLGACVDVLGGLIHIGIGG 285

Query: 153 PAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLI-SCGKTPPPGYTC 195
            A   CCP+L GL +++AA+CLCTT+++K L++NI +PIALQ+LI  CGK PP  + C
Sbjct: 286 SAKQTCCPLLGGLVDLDAAICLCTTIRLKLLNINIILPIALQVLIDDCGKYPPKDFKC 343

[Close]

BLAST of Carg12111 vs. ExPASy Swiss-Prot
Match: Q9SU34 (pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12490 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.8e-18
Identity = 56/152 (36.84%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 45  SPKGGPIGLPPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKP 104
           SPK  P+  P +  P V  P +  P +  P V  P +P  ++P  +VP     P+P   P
Sbjct: 33  SPKPRPLPNPKVPSPKVPTPSVPSPYVPTPSVPSPSVPTPSVPSPSVPS--PNPTPVIPP 92

Query: 105 CPPSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLK 164
             P     CPID L+LG C ++L GL+++ LG P+   CC ++ GL +++AAVCLCT L+
Sbjct: 93  RTPGSSGNCPIDALRLGVCANVLSGLLNVQLGQPSPQPCCSLIQGLVDLDAAVCLCTALR 152

Query: 165 IKALSLNIYVPIALQLLIS-CGKTPPPGYTCS 196
              L +N+ VPI+L +L++ C +  P  + C+
Sbjct: 153 ANVLGINLNVPISLSVLLNVCNRRLPSNFQCA 182

[Close]

BLAST of Carg12111 vs. ExPASy Swiss-Prot
Match: Q9SU35 (pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.9e-17
Identity = 56/133 (42.11%), Postives = 80/133 (60.15%), Query Frame = 0


Query: 70  PIVKP-PVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPP-----SGKETCPIDTLKLGAC 129
           P  KP PV  P   PV  PP   PP  + PSP  +P  P     S   +CPID LKLG C
Sbjct: 32  PSPKPKPVPSPKPKPVQCPP---PPRPSVPSPNPRPVTPPRTPGSSGNSCPIDALKLGVC 91

Query: 130 VDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS 189
            ++L  L++I LG P+  +CC ++ GL +V+AA+CLCT L+   L +N+ VPI+L +L++
Sbjct: 92  ANVLSSLLNIQLGQPSSQQCCSLIQGLVDVDAAICLCTALRANVLGINLNVPISLSVLLN 151

Query: 190 -CGKTPPPGYTCS 196
            C +  P G+ C+
Sbjct: 152 VCNRKLPSGFQCA 161

[Close]

BLAST of Carg12111 vs. ExPASy Swiss-Prot
Match: Q9SU33 (pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g12500 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 8.6e-17
Identity = 51/138 (36.96%), Postives = 80/138 (57.97%), Query Frame = 0


Query: 59  PPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTL 118
           P V  P    P I  P V  P +P  ++P  +VP     P+P + P  P     CPID L
Sbjct: 42  PKVPSPKYPSPSIPSPSVPTPSVPTPSVPTPSVPS--PNPTPVTPPRTPGSSGNCPIDAL 101

Query: 119 KLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIAL 178
           +LG C ++L GL+++ LG P+   CC ++ GL +++AA+CLCT L+   L +N+ VPI+L
Sbjct: 102 RLGVCANVLSGLLNVQLGQPSAQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISL 161

Query: 179 QLLIS-CGKTPPPGYTCS 196
            +L++ C +  P  + C+
Sbjct: 162 SVLLNVCNRRLPSDFQCA 177

[Close]

BLAST of Carg12111 vs. ExPASy Swiss-Prot
Match: Q39176 (Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.5e-16
Identity = 65/195 (33.33%), Postives = 97/195 (49.74%), Query Frame = 0


Query: 2   ESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPPV 61
           ++S   +LF    +   T T    CG   NP PK  KP     SPK  P+          
Sbjct: 4   KNSASIALFFALNIIFFTLTAATDCGC--NPSPKH-KP---VPSPKPKPV---------- 63

Query: 62  VKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLG 121
                   P  KP     P+P  ++P  +VP     P P + P  P     CPID L+LG
Sbjct: 64  --------PSPKP----KPVPSPSVPSPSVPS--PNPRPVTPPRTPGSSGNCPIDALRLG 123

Query: 122 ACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLL 181
            C ++L  L++I LG P+   CC ++ GL +++AA+CLCT L+   L +N+ VPI+L +L
Sbjct: 124 VCANVLSSLLNIQLGQPSAQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISLSVL 168

Query: 182 IS-CGKTPPPGYTCS 196
           ++ C +  P G+ C+
Sbjct: 184 LNVCNRKVPSGFQCA 168

[Close]

BLAST of Carg12111 vs. ExPASy TrEMBL
Match: A0A6J1F602 (36.4 kDa proline-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111441146 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.2e-99
Identity = 194/206 (94.17%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL------- 60
           MESSKIYSLFLVCMLFLSTATPI GCGACGNPPPKKGKPGSGSKSPKGGPIGL       
Sbjct: 1   MESSKIYSLFLVCMLFLSTATPIFGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPITLPP 60

Query: 61  ---PPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120
              PPIDLPPVVKPPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGK
Sbjct: 61  IVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120

Query: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180
           ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL
Sbjct: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180

Query: 181 NIYVPIALQLLISCGKTPPPGYTCSL 197
           NIYVPIALQLLISCGKTPPPGYTCSL
Sbjct: 181 NIYVPIALQLLISCGKTPPPGYTCSL 206

[Close]

BLAST of Carg12111 vs. ExPASy TrEMBL
Match: A0A6J1HPJ2 (36.4 kDa proline-rich protein-like OS=Cucurbita maxima OX=3661 GN=LOC111466144 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.8e-97
Identity = 192/206 (93.20%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGL------- 60
           MESSKIYSLFL+CMLFLSTATPI GCGACGNPPPKK KPGSGSKSPKGGPIGL       
Sbjct: 1   MESSKIYSLFLLCMLFLSTATPIFGCGACGNPPPKKVKPGSGSKSPKGGPIGLPPISLPP 60

Query: 61  ---PPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120
              PPIDLPPVVKPPISLPPIVKPPVDLPP+PPVTLPPVTVPPVLTKPSPASKPCPPSGK
Sbjct: 61  IVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGK 120

Query: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180
           ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL
Sbjct: 121 ETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSL 180

Query: 181 NIYVPIALQLLISCGKTPPPGYTCSL 197
           NIYVPIALQLLISCGKTPPPGYTCSL
Sbjct: 181 NIYVPIALQLLISCGKTPPPGYTCSL 206

[Close]

BLAST of Carg12111 vs. ExPASy TrEMBL
Match: A0A6J1HBI4 (36.4 kDa proline-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111461860 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.2e-75
Identity = 163/235 (69.36%), Postives = 177/235 (75.32%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKG---GPIGL---- 60
           ME SKI++L LVCMLFLSTA PI GCG+CG PPPKK KPGSGSKSPKG    PI L    
Sbjct: 1   MEPSKIHALLLVCMLFLSTAAPIFGCGSCGKPPPKKVKPGSGSKSPKGPIVPPITLPPVV 60

Query: 61  ---------------PPIDLPPVVKPPISLPPI----VKPPVDLP------------PIP 120
                          PPIDLPPVVKPPI +PP+    VKPP+DLP            PIP
Sbjct: 61  KPPIKIPPVPLPPVKPPIDLPPVVKPPIKIPPVPLPPVKPPIDLPPVIKPPIKIPPVPIP 120

Query: 121 PVTLPPVTVPPVLTKPSPASKPC-PPSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVN 180
           PVT+PPVT PPV+  PSP   PC PP GKETCPIDTLKLG CVDLLGGLVHIG+GDPA N
Sbjct: 121 PVTVPPVTAPPVIKNPSPGKNPCPPPPGKETCPIDTLKLGGCVDLLGGLVHIGVGDPAAN 180

Query: 181 ECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTCSL 197
            CCP++SGLAE+EAAVCLCTTLKIKAL+LNIYVPIALQLLI+CGKTPPPGYTCSL
Sbjct: 181 ACCPIISGLAELEAAVCLCTTLKIKALNLNIYVPIALQLLITCGKTPPPGYTCSL 235

[Close]

BLAST of Carg12111 vs. ExPASy TrEMBL
Match: A0A6J1CTE9 (36.4 kDa proline-rich protein OS=Momordica charantia OX=3673 GN=LOC111014420 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.5e-75
Identity = 157/212 (74.06%), Postives = 176/212 (83.02%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPI---- 60
           ME SK++SL L+CM+ +S+A P+LGCG+CGN PPKK KP  GSKSPK GPIGLPP+    
Sbjct: 1   MEPSKLHSLLLICMVLVSSAAPMLGCGSCGN-PPKKHKPSPGSKSPK-GPIGLPPVIVKP 60

Query: 61  --DLPPV----VKPPISLPPIVKPPVDLPPI------PPVTLPPVTVPPVLTKPSPASKP 120
              LPP+    VKPP+ LPP+  PPV +PP+      PPV LPPVTVPPVL KPSP+ KP
Sbjct: 61  PGGLPPIGLPPVKPPVDLPPVGLPPVTVPPVKPPVDLPPVGLPPVTVPPVLGKPSPSGKP 120

Query: 121 CPPSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLK 180
           CPP GKETCPIDTLKLG CVDLLGGLVHIGLGDPA NECCPVL+GLAEVEAAVCLCTTLK
Sbjct: 121 CPPKGKETCPIDTLKLGGCVDLLGGLVHIGLGDPAANECCPVLAGLAEVEAAVCLCTTLK 180

Query: 181 IKALSLNIYVPIALQLLISCGKTPPPGYTCSL 197
           IKAL+LN+YVPIALQLLI+CGKTPPPGYTCSL
Sbjct: 181 IKALNLNLYVPIALQLLITCGKTPPPGYTCSL 210

[Close]

BLAST of Carg12111 vs. ExPASy TrEMBL
Match: A0A0A0LT87 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G228960 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.7e-69
Identity = 152/205 (74.15%), Postives = 172/205 (83.90%), Query Frame = 0


Query: 1   MESSKIYSLFLVCMLFLSTATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLP--PIDL 60
           ME SKIYSLFL+CMLF+S+A+PILGCG+C   PP+K KPGSGSKSPK GPI LP  PI +
Sbjct: 1   MEPSKIYSLFLICMLFISSASPILGCGSCSGKPPRKTKPGSGSKSPK-GPITLPPIPIKI 60

Query: 61  PPVVK-PPISLPPIVK-PPVDLP---PIPPVTLPPV-TVPPVLTKPSP-ASKPCPPSGKE 120
           PPVV  P + +PP+V  P V +P   PIP V +PPV  VPP+L KPSP  + PCPPSGKE
Sbjct: 61  PPVVPIPSVPIPPVVPIPSVPIPPVVPIPSVPIPPVLPVPPILGKPSPGVNTPCPPSGKE 120

Query: 121 TCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLN 180
           TCPIDTLKLG CV+LLGGLVHIG+GDPA N CCP++SGLAE+EAAVCLCTTLKIKAL LN
Sbjct: 121 TCPIDTLKLGGCVNLLGGLVHIGIGDPAANACCPIISGLAELEAAVCLCTTLKIKALDLN 180

Query: 181 IYVPIALQLLISCGKTPPPGYTCSL 197
           IYVPIALQLLI+CGKTPPPGYTCSL
Sbjct: 181 IYVPIALQLLITCGKTPPPGYTCSL 204

[Close]

BLAST of Carg12111 vs. TAIR 10
Match: AT2G10940.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-44
Identity = 97/172 (56.40%), Postives = 117/172 (68.02%), Query Frame = 0


Query: 50  PIGLPPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVT---------------VPPV 109
           P+ +P + +PPV  P + +PP+  P + LPPI  + +PPV                +PP 
Sbjct: 120 PVTVPKLPVPPVTVPKLPVPPVTVPKLPLPPISGLPIPPVVGPNLPLPPLPIVGPILPPG 179

Query: 110 LTKPSPASKPCPP----------SGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECC 169
            T P+   K CPP           GK TCPIDTLKLGACVDLLGGLV IGLGDPAVN+CC
Sbjct: 180 TTPPATGGKDCPPPPGSVKPPSGGGKATCPIDTLKLGACVDLLGGLVKIGLGDPAVNKCC 239

Query: 170 PVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTCSL 197
           P+L GL EVEAA CLCTTLK+KAL LN+YVP+ALQLL++CGK PPPGYTCS+
Sbjct: 240 PLLKGLVEVEAAACLCTTLKLKALDLNLYVPVALQLLLTCGKNPPPGYTCSI 291

[Close]

BLAST of Carg12111 vs. TAIR 10
Match: AT2G10940.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-44
Identity = 97/172 (56.40%), Postives = 117/172 (68.02%), Query Frame = 0


Query: 50  PIGLPPIDLPPVVKPPISLPPIVKPPVDLPPIPPVTLPPVT---------------VPPV 109
           P+ +P + +PPV  P + +PP+  P + LPPI  + +PPV                +PP 
Sbjct: 120 PVTVPKLPVPPVTVPKLPVPPVTVPKLPLPPISGLPIPPVVGPNLPLPPLPIVGPILPPG 179

Query: 110 LTKPSPASKPCPP----------SGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECC 169
            T P+   K CPP           GK TCPIDTLKLGACVDLLGGLV IGLGDPAVN+CC
Sbjct: 180 TTPPATGGKDCPPPPGSVKPPSGGGKATCPIDTLKLGACVDLLGGLVKIGLGDPAVNKCC 239

Query: 170 PVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTCSL 197
           P+L GL EVEAA CLCTTLK+KAL LN+YVP+ALQLL++CGK PPPGYTCS+
Sbjct: 240 PLLKGLVEVEAAACLCTTLKLKALDLNLYVPVALQLLLTCGKNPPPGYTCSI 291

[Close]

BLAST of Carg12111 vs. TAIR 10
Match: AT3G22142.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 3.1e-38
Identity = 93/195 (47.69%), Postives = 121/195 (62.05%), Query Frame = 0


Query: 19   TATPILGCGACGNPPPKKGKPGSGSKSPKGGPIGLPPIDLPPVVKPPISLPPIVKPPVDL 78
            TATP +       PP         +  P   P+  PP+  PP V PP++ PPI KPP   
Sbjct: 1291 TATPPVATSPIAKPP-------VATPPPATPPVAKPPVATPPTVTPPVATPPIAKPPGAR 1350

Query: 79   PPI--PPVTLPPVTVPPV----LTKPSPASKP--CPPSGK-----------ETCPIDTLK 138
            PP+  PPV  PP+   PV    +TKP  AS P   PP  K           +TCPIDTLK
Sbjct: 1351 PPVATPPVATPPIAKSPVATPPMTKPPVASSPIATPPIAKTPIATPPTTMPKTCPIDTLK 1410

Query: 139  LGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQ 195
            LG+CVDLLGGLVHIG+G  A  +CCPV+ GL +++AAVCLCTT+K K L++++ +PIAL+
Sbjct: 1411 LGSCVDLLGGLVHIGIGKSAKEKCCPVVEGLVDLDAAVCLCTTIKAKLLNIDVILPIALE 1470

[Close]

BLAST of Carg12111 vs. TAIR 10
Match: AT1G62500.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 5.3e-38
Identity = 92/168 (54.76%), Postives = 110/168 (65.48%), Query Frame = 0


Query: 50  PIGLPPIDLPPVVKPPISLPPIVKPPVDLPPI--PPVTLPPVTVPPVL----TKPSPASK 109
           PI +PPI  PPV  PP  LPPI  PP  LPP+  PP  LPPVT PP L      P P + 
Sbjct: 126 PIIIPPIQPPPVTTPPGLLPPITTPPGLLPPVTTPPGLLPPVTTPPGLLPPIINPPPVTV 185

Query: 110 PCPPSG-----------------KETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPV 169
           P P SG                 + TCPI+ LKLGACVD+LGGL+HIGLG+P  N CCPV
Sbjct: 186 PPPSSGYPPYGPPSGGGGGGGGKQPTCPINALKLGACVDVLGGLIHIGLGNPVENVCCPV 245

Query: 170 LSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTC 195
           L GL E+EAAVCLCTT+++K L+LNI++P+ALQ LI+CG  PP G+ C
Sbjct: 246 LQGLLELEAAVCLCTTIRLKLLNLNIFIPLALQALITCGINPPSGFVC 293

[Close]

BLAST of Carg12111 vs. TAIR 10
Match: AT3G22120.1 (cell wall-plasma membrane linker protein )

HSP 1 Score: 154.1 bits (388), Expect = 1.2e-37
Identity = 91/178 (51.12%), Postives = 116/178 (65.17%), Query Frame = 0


Query: 32  PPPKKGKPGSGSKSPKGGPIGLPPIDLPPVVKPPISLPPIVKPPVDLPPI-------PPV 91
           PPP    P + + +P   P+  PP   PPV+ PP   PP+V PP   PP+       PPV
Sbjct: 158 PPPTPCPPPTPTPTP---PVVTPPTPTPPVITPPTPTPPVVTPPTPTPPVITPPTPTPPV 217

Query: 92  TLPPVTVPPVLTKPSPA------SKPCPPSG-KETCPIDTLKLGACVDLLGGLVHIGLG- 151
             PP   PPV+T P+P         P PP+   ETCPIDTLKLGACVD+LGGL+HIGLG 
Sbjct: 218 ITPPTPTPPVVTPPTPTPPVVTPPTPTPPTPIPETCPIDTLKLGACVDVLGGLIHIGLGK 277

Query: 152 DPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTC 195
             A  ECCPVL GL +++AAVCLCTT+K+K L++++ +PIAL+LL+ CGKTPP  + C
Sbjct: 278 SHAKAECCPVLGGLLDLDAAVCLCTTIKLKLLNIDLVLPIALELLLDCGKTPPSDFKC 332

The following BLAST results are available for this feature:

BLAST of Carg12111 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021766.1	1.1e-101	100.00	pEARLI1-like lipid transfer protein 1, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_023529211.1	1.1e-98	94.17	36.4 kDa proline-rich protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022933855.1	1.5e-98	94.17	36.4 kDa proline-rich protein-like [Cucurbita moschata]	[more]
XP_022966511.1	3.6e-97	93.20	36.4 kDa proline-rich protein-like [Cucurbita maxima]	[more]
KAG6587881.1	7.9e-92	94.30	pEARLI1-like lipid transfer protein 1, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of Carg12111 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00451	2.8e-31	51.12	36.4 kDa proline-rich protein OS=Solanum lycopersicum OX=4081 GN=TPRP-F1 PE=2 SV...	[more]
Q9SU34	7.8e-18	36.84	pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g124...	[more]
Q9SU35	3.9e-17	42.11	pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE...	[more]
Q9SU33	8.6e-17	36.96	pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g125...	[more]
Q39176	2.5e-16	33.33	Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV...	[more]

BLAST of Carg12111 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F602	7.2e-99	94.17	36.4 kDa proline-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111441146...	[more]
A0A6J1HPJ2	1.8e-97	93.20	36.4 kDa proline-rich protein-like OS=Cucurbita maxima OX=3661 GN=LOC111466144 P...	[more]
A0A6J1HBI4	3.2e-75	69.36	36.4 kDa proline-rich protein-like OS=Cucurbita moschata OX=3662 GN=LOC111461860...	[more]
A0A6J1CTE9	5.5e-75	74.06	36.4 kDa proline-rich protein OS=Momordica charantia OX=3673 GN=LOC111014420 PE=...	[more]
A0A0A0LT87	2.7e-69	74.15	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G228960 PE=3 SV...	[more]

BLAST of Carg12111 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G10940.1	2.2e-44	56.40	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G10940.2	2.2e-44	56.40	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G22142.1	3.1e-38	47.69	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G62500.1	5.3e-38	54.76	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G22120.1	1.2e-37	51.12	cell wall-plasma membrane linker protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 113..194 e-value: 2.5E-5 score: 33.7
IPR027923	Hydrophobic seed protein domain	PFAM	PF14547	Hydrophob_seed	coord: 112..194 e-value: 1.9E-27 score: 95.3
IPR027923	Hydrophobic seed protein domain	CDD	cd01958	HPS_like	coord: 112..194 e-value: 7.04931E-26 score: 93.5472
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 112..195 e-value: 1.4E-22 score: 81.3
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 115..195
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..58
None	No IPR available	PANTHER	PTHR31731:SF1	36.4 KDA PROLINE-RICH PROTEIN	coord: 1..96 coord: 102..195
None	No IPR available	PANTHER	PTHR31731	FAMILY NOT NAMED	coord: 1..96 coord: 102..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12111-RA	Carg12111-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane