Carg11992 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11992
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	PMEI domain-containing protein
Location	Carg_Chr05: 9043292 .. 9043825 (-)
RNA-Seq Expression	Carg11992
Synteny	Carg11992

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTGCTCTCATGGCATCCCTGTTCTTCTTCTGGCAGTTTTTTACCTAGCCATTTTCCCTTCTCATGGAATCCTCCATGGAAATCTGGCCACCATTTGCTATAAAACCCCCGATCCGTTCAGCTGCCAGAGAATCCTTGGTAACGTTCCCGGTACCATCTCGGCTGACCTCCCTAAGCTCTCGCTTATATCCATCAATCTGACGAAGAACCAAGCCGATCAAAACCTTGATTCCTTCTCCAAATTAAGTAAGAATGAGAGTGATCCTGAGCTGAAGAAGAGTTTCAAGCATTGTGTGAAGTATTACCATGAAATTCAAGCACATATCCAGAAAGCCTTTAAGCTCTCACGGAAGAAGATTTTCAGAGATAATCATCCATTGGTAACATCAAAGAAACTTGTTAAAAGGTGTAATCAAGCCATCAGAATCAACTCATTGGATATTGAAGTCATGAACAAACGTATGATCCTAAGCTGTGATATTTCAATGAGTGTAAATCACTATGCTACTGCTAATGCCCACATTCAGTAA

mRNA sequence

ATGGGTTGCTCTCATGGCATCCCTGTTCTTCTTCTGGCAGTTTTTTACCTAGCCATTTTCCCTTCTCATGGAATCCTCCATGGAAATCTGGCCACCATTTGCTATAAAACCCCCGATCCGTTCAGCTGCCAGAGAATCCTTGGTAACGTTCCCGGTACCATCTCGGCTGACCTCCCTAAGCTCTCGCTTATATCCATCAATCTGACGAAGAACCAAGCCGATCAAAACCTTGATTCCTTCTCCAAATTAAGTAAGAATGAGAGTGATCCTGAGCTGAAGAAGAGTTTCAAGCATTGTGTGAAGTATTACCATGAAATTCAAGCACATATCCAGAAAGCCTTTAAGCTCTCACGGAAGAAGATTTTCAGAGATAATCATCCATTGGTAACATCAAAGAAACTTGTTAAAAGGTGTAATCAAGCCATCAGAATCAACTCATTGGATATTGAAGTCATGAACAAACGTATGATCCTAAGCTGTGATATTTCAATGAGTGTAAATCACTATGCTACTGCTAATGCCCACATTCAGTAA

Coding sequence (CDS)

ATGGGTTGCTCTCATGGCATCCCTGTTCTTCTTCTGGCAGTTTTTTACCTAGCCATTTTCCCTTCTCATGGAATCCTCCATGGAAATCTGGCCACCATTTGCTATAAAACCCCCGATCCGTTCAGCTGCCAGAGAATCCTTGGTAACGTTCCCGGTACCATCTCGGCTGACCTCCCTAAGCTCTCGCTTATATCCATCAATCTGACGAAGAACCAAGCCGATCAAAACCTTGATTCCTTCTCCAAATTAAGTAAGAATGAGAGTGATCCTGAGCTGAAGAAGAGTTTCAAGCATTGTGTGAAGTATTACCATGAAATTCAAGCACATATCCAGAAAGCCTTTAAGCTCTCACGGAAGAAGATTTTCAGAGATAATCATCCATTGGTAACATCAAAGAAACTTGTTAAAAGGTGTAATCAAGCCATCAGAATCAACTCATTGGATATTGAAGTCATGAACAAACGTATGATCCTAAGCTGTGATATTTCAATGAGTGTAAATCACTATGCTACTGCTAATGCCCACATTCAGTAA

Protein sequence

MGCSHGIPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISINLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNHPLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ

Homology

[Close]

BLAST of Carg11992 vs. NCBI nr
Match: KAG6599172.1 (hypothetical protein SDJN03_08950, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030110.1 hypothetical protein SDJN02_08457, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 353.2 bits (905), Expect = 1.3e-93
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MGCSHGIPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPK 60
           MGCSHGIPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPK
Sbjct: 1   MGCSHGIPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPK 60

Query: 61  LSLISINLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKK 120
           LSLISINLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKK
Sbjct: 61  LSLISINLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKK 120

Query: 121 IFRDNHPLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ 178
           IFRDNHPLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ
Sbjct: 121 IFRDNHPLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ 177

[Close]

BLAST of Carg11992 vs. NCBI nr
Match: KAA0048111.1 (pectinesterase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 235.7 bits (600), Expect = 3.0e-58
Identity = 122/171 (71.35%), Postives = 140/171 (81.87%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           IP LLLA F L IFPSHGI H NL TIC KT +P  C++IL N   T+SADLPKLSLI  
Sbjct: 11  IPALLLATFSLTIFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSADLPKLSLICS 70

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
           NL K QADQNLD+F KLSKNESDPE+KKSF+HCV+YYHEIQ++IQKA++ S++KIFR+N 
Sbjct: 71  NLAKKQADQNLDTFYKLSKNESDPEVKKSFEHCVEYYHEIQSNIQKAYQFSQQKIFRENG 130

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ 178
           PLV SK+LV RC+QAIRINSL IEVMNK MIL CDIS+SVN  A AN HIQ
Sbjct: 131 PLVASKRLVLRCSQAIRINSLHIEVMNKLMILCCDISISVNQCAAANGHIQ 181

[Close]

BLAST of Carg11992 vs. NCBI nr
Match: KAE8651630.1 (hypothetical protein Csa_021165 [Cucumis sativus])

HSP 1 Score: 228.0 bits (580), Expect = 6.3e-56
Identity = 118/169 (69.82%), Postives = 137/169 (81.07%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           IP LLLA F L +FPSHGI H NL TIC KT +P  C++IL N   T+SA+LPKLSLI  
Sbjct: 500 IPALLLAAFSLTVFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSANLPKLSLICS 559

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
           NL K QADQNLD+F KLSKNESDPE KKSF+HCVKYYHEIQ++IQKA++ S++KIFR+N 
Sbjct: 560 NLAKKQADQNLDTFYKLSKNESDPEEKKSFEHCVKYYHEIQSNIQKAYQFSQQKIFRENG 619

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAH 176
           PLV SK+LV +C+QAIRINSL IEVMNK MIL CDIS+SVN  A AN H
Sbjct: 620 PLVASKRLVLKCSQAIRINSLYIEVMNKLMILCCDISISVNQCAAANGH 668

[Close]

BLAST of Carg11992 vs. NCBI nr
Match: KAB1201343.1 (Pectinesterase inhibitor [Morella rubra] >KAB1224060.1 Pectinesterase inhibitor [Morella rubra])

HSP 1 Score: 127.9 bits (320), Expect = 8.8e-26
Identity = 73/163 (44.79%), Postives = 103/163 (63.19%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           + V +L+V    I+PS G+ H  L TIC KT +  +C+R L +   T SADLPKLSLISI
Sbjct: 8   VSVTVLSVASFLIYPSSGVSHDILTTICSKTQNHDACERSLESDSRTRSADLPKLSLISI 67

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
            LT  QAD N  +FS L  N +DP L+ SF++CV +Y E+QA I+ A+ LS+K+ ++   
Sbjct: 68  ELTMKQADHNHRTFSDLRDNATDPRLEDSFRNCVGFYKEMQAQIRAAYHLSQKRQYQKIT 127

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHY 170
            LV S++L + C   I IN L+I+ +   M+L+   S SVN Y
Sbjct: 128 ELVQSERLAQECASGIPINCLEIDALTTTMLLAIKTSESVNQY 170

[Close]

BLAST of Carg11992 vs. NCBI nr
Match: VVA33128.1 (PREDICTED: pectinesterase inhibitor [Prunus dulcis])

HSP 1 Score: 118.2 bits (295), Expect = 7.0e-23
Identity = 64/161 (39.75%), Postives = 102/161 (63.35%), Query Frame = 0


Query: 9   VLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISINL 68
           ++++A F++  F   G+ +  L  IC KT +P +C   L  VP T SADLP+LSLISI L
Sbjct: 12  IVIVASFFIGSF---GVSYKTLTKICSKTQNPKTCMTNLQTVPCTTSADLPELSLISIEL 71

Query: 69  TKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNHPL 128
              QADQNL++F +LS N +  ++K+ F  CV++Y E++  IQ A  LS+K+ + +N  L
Sbjct: 72  AGKQADQNLETFRELSHNATAAKMKEEFDTCVRFYKEVRPQIQAAHLLSQKRQYENNSAL 131

Query: 129 VTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHY 170
           + + +   RC+  I ++SL+I  + + ++L  D S+SVN Y
Sbjct: 132 LQANEFALRCSLGISVDSLEINQLQESVLLKLDTSISVNRY 169

[Close]

BLAST of Carg11992 vs. ExPASy TrEMBL
Match: A0A5A7U3I1 (Pectinesterase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001900 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.5e-58
Identity = 122/171 (71.35%), Postives = 140/171 (81.87%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           IP LLLA F L IFPSHGI H NL TIC KT +P  C++IL N   T+SADLPKLSLI  
Sbjct: 11  IPALLLATFSLTIFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSADLPKLSLICS 70

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
           NL K QADQNLD+F KLSKNESDPE+KKSF+HCV+YYHEIQ++IQKA++ S++KIFR+N 
Sbjct: 71  NLAKKQADQNLDTFYKLSKNESDPEVKKSFEHCVEYYHEIQSNIQKAYQFSQQKIFRENG 130

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAHIQ 178
           PLV SK+LV RC+QAIRINSL IEVMNK MIL CDIS+SVN  A AN HIQ
Sbjct: 131 PLVASKRLVLRCSQAIRINSLHIEVMNKLMILCCDISISVNQCAAANGHIQ 181

[Close]

BLAST of Carg11992 vs. ExPASy TrEMBL
Match: A0A0A0LK13 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034700 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 8.8e-56
Identity = 117/169 (69.23%), Postives = 136/169 (80.47%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           IP LLLA F L +FPSHGI H NL TIC KT +P  C++IL N   T+SA+LPKLSLI  
Sbjct: 11  IPALLLAAFSLTVFPSHGIPHENLVTICSKTSNPSLCEKILNNDSRTVSANLPKLSLICS 70

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
           NL K QADQNLD+F KLSKNESDPE KKSF+HCVKYYHEIQ++IQKA++ S++KIFR+N 
Sbjct: 71  NLAKKQADQNLDTFYKLSKNESDPEEKKSFEHCVKYYHEIQSNIQKAYQFSQQKIFRENG 130

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHYATANAH 176
           PLV SK+LV +C+Q IRINSL IEVMNK MIL CDIS+SVN  A AN H
Sbjct: 131 PLVASKRLVLKCSQTIRINSLYIEVMNKLMILCCDISISVNQCAAANGH 179

[Close]

BLAST of Carg11992 vs. ExPASy TrEMBL
Match: A0A6A1UMD5 (Pectinesterase inhibitor OS=Morella rubra OX=262757 GN=CJ030_MR0G004165 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.3e-26
Identity = 73/163 (44.79%), Postives = 103/163 (63.19%), Query Frame = 0


Query: 7   IPVLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISI 66
           + V +L+V    I+PS G+ H  L TIC KT +  +C+R L +   T SADLPKLSLISI
Sbjct: 8   VSVTVLSVASFLIYPSSGVSHDILTTICSKTQNHDACERSLESDSRTRSADLPKLSLISI 67

Query: 67  NLTKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNH 126
            LT  QAD N  +FS L  N +DP L+ SF++CV +Y E+QA I+ A+ LS+K+ ++   
Sbjct: 68  ELTMKQADHNHRTFSDLRDNATDPRLEDSFRNCVGFYKEMQAQIRAAYHLSQKRQYQKIT 127

Query: 127 PLVTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHY 170
            LV S++L + C   I IN L+I+ +   M+L+   S SVN Y
Sbjct: 128 ELVQSERLAQECASGIPINCLEIDALTTTMLLAIKTSESVNQY 170

[Close]

BLAST of Carg11992 vs. ExPASy TrEMBL
Match: A0A5E4G0J4 (PREDICTED: pectinesterase inhibitor OS=Prunus dulcis OX=3755 GN=ALMOND_2B005171 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 3.4e-23
Identity = 64/161 (39.75%), Postives = 102/161 (63.35%), Query Frame = 0


Query: 9   VLLLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISINL 68
           ++++A F++  F   G+ +  L  IC KT +P +C   L  VP T SADLP+LSLISI L
Sbjct: 12  IVIVASFFIGSF---GVSYKTLTKICSKTQNPKTCMTNLQTVPCTTSADLPELSLISIEL 71

Query: 69  TKNQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNHPL 128
              QADQNL++F +LS N +  ++K+ F  CV++Y E++  IQ A  LS+K+ + +N  L
Sbjct: 72  AGKQADQNLETFRELSHNATAAKMKEEFDTCVRFYKEVRPQIQAAHLLSQKRQYENNSAL 131

Query: 129 VTSKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHY 170
           + + +   RC+  I ++SL+I  + + ++L  D S+SVN Y
Sbjct: 132 LQANEFALRCSLGISVDSLEINQLQESVLLKLDTSISVNRY 169

[Close]

BLAST of Carg11992 vs. ExPASy TrEMBL
Match: A0A438CFW3 (PMEI domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_097019 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.0e-19
Identity = 65/159 (40.88%), Postives = 93/159 (58.49%), Query Frame = 0


Query: 11  LLAVFYLAIFPSHGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISINLTK 70
           LL VF L   P+ G+    LA+IC +T +   C+ IL + PGT SADL  LSLISINLT 
Sbjct: 14  LLMVFSLFTSPTSGVTQETLASICSQTQNQEICEGILESDPGTTSADLHGLSLISINLTM 73

Query: 71  NQADQNLDSFSKLSKNESDPELKKSFKHCVKYYHEIQAHIQKAFKLSRKKIFRDNHPLVT 130
            Q   N D+F+K   N +D  LK+SF++CV  Y+ I   ++ A++LS KK +        
Sbjct: 74  KQGSSNYDTFTKFMDNSTDTALKESFRNCVSLYNSIMEMLKVAYQLSEKKEYESITQPGE 133

Query: 131 SKKLVKRCNQAIRINSLDIEVMNKRMILSCDISMSVNHY 170
           S+ L   C   + I+S   + +++ MIL+C+ S SVN Y
Sbjct: 134 SQTLAYNCANGLPIDS-PTDGISRDMILTCETSASVNQY 171

The following BLAST results are available for this feature:

BLAST of Carg11992 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6599172.1	1.3e-93	100.00	hypothetical protein SDJN03_08950, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0048111.1	3.0e-58	71.35	pectinesterase inhibitor [Cucumis melo var. makuwa]	[more]
KAE8651630.1	6.3e-56	69.82	hypothetical protein Csa_021165 [Cucumis sativus]	[more]
KAB1201343.1	8.8e-26	44.79	Pectinesterase inhibitor [Morella rubra] >KAB1224060.1 Pectinesterase inhibitor ...	[more]
VVA33128.1	7.0e-23	39.75	PREDICTED: pectinesterase inhibitor [Prunus dulcis]	[more]

BLAST of Carg11992 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg11992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U3I1	1.5e-58	71.35	Pectinesterase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A0A0LK13	8.8e-56	69.23	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034700 PE=4 S...	[more]
A0A6A1UMD5	4.3e-26	44.79	Pectinesterase inhibitor OS=Morella rubra OX=262757 GN=CJ030_MR0G004165 PE=4 SV=...	[more]
A0A5E4G0J4	3.4e-23	39.75	PREDICTED: pectinesterase inhibitor OS=Prunus dulcis OX=3755 GN=ALMOND_2B005171 ...	[more]
A0A438CFW3	1.0e-19	40.88	PMEI domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_097019 PE=4 S...	[more]

BLAST of Carg11992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 25..166 e-value: 0.0027 score: 23.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 17..124 e-value: 2.3E-15 score: 54.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..117 e-value: 3.2E-9 score: 37.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 29..169 e-value: 1.4E-17 score: 66.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..124
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 10..146
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 10..146
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 32..169 e-value: 1.99124E-21 score: 83.2405

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11992-RA	Carg11992-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity