Carg11895 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11895
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPhytosulfokine-beta
LocationCarg_Chr05: 582167 .. 582532 (+)
RNA-Seq ExpressionCarg11895
SyntenyCarg11895
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCACGAATATGTCGAAGCTCGCGGCTCTCTTCACCATTGCAGCACTCGTTTCAATGTCCCTCATGCTGCCGTCCTCCGAGGCTCGTCCCATCACTACTCTCACTGCCACTTCTGCCGTAATCGCCAATCCGGTAACGTAAAGTTTCATATCTCCGTTTTAATTTCGGATTGACTATGATATTGTTAAACTAAATTTGATGCTTAATTCTACGTATCCCTCCTAGGTGATCGTTTCTGAAGAGGTTTGTAAAGACGACTGCCTGATCAGAAGAACCTTAGAGGCTCATACTGATTACATCTATTCCGGCGACACTAAGCCATGAATTACTTATCTTTTTATATTTTCTCTCATTAATACAGTCTA

mRNA sequence

AACCACGAATATGTCGAAGCTCGCGGCTCTCTTCACCATTGCAGCACTCGTTTCAATGTCCCTCATGCTGCCGTCCTCCGAGGCTCGTCCCATCACTACTCTCACTGCCACTTCTGCCGTAATCGCCAATCCGGTGATCGTTTCTGAAGAGGTTTGTAAAGACGACTGCCTGATCAGAAGAACCTTAGAGGCTCATACTGATTACATCTATTCCGGCGACACTAAGCCATGAATTACTTATCTTTTTATATTTTCTCTCATTAATACAGTCTA

Coding sequence (CDS)

ATGTCGAAGCTCGCGGCTCTCTTCACCATTGCAGCACTCGTTTCAATGTCCCTCATGCTGCCGTCCTCCGAGGCTCGTCCCATCACTACTCTCACTGCCACTTCTGCCGTAATCGCCAATCCGGTGATCGTTTCTGAAGAGGTTTGTAAAGACGACTGCCTGATCAGAAGAACCTTAGAGGCTCATACTGATTACATCTATTCCGGCGACACTAAGCCATGA

Protein sequence

MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEVCKDDCLIRRTLEAHTDYIYSGDTKP
Homology
BLAST of Carg11895 vs. NCBI nr
Match: KAG6598323.1 (Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029293.1 Phytosulfokine [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137.1 bits (344), Expect = 6.0e-29
Identity = 73/73 (100.00%), Postives = 73/73 (100.00%), Query Frame = 0

Query: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEVCKDDCLIRRTLE 60
          MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEVCKDDCLIRRTLE
Sbjct: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEVCKDDCLIRRTLE 60

Query: 61 AHTDYIYSGDTKP 74
          AHTDYIYSGDTKP
Sbjct: 61 AHTDYIYSGDTKP 73

BLAST of Carg11895 vs. NCBI nr
Match: XP_023546620.1 (phytosulfokines-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 99.8 bits (247), Expect = 1.1e-17
Identity = 63/82 (76.83%), Postives = 66/82 (80.49%), Query Frame = 0

Query: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEV-----C----KD 60
          MSKLAALFTIAAL++M     SSEARPI TLTATSAVIANPV V+EEV     C    KD
Sbjct: 1  MSKLAALFTIAALLTM-----SSEARPIATLTATSAVIANPV-VAEEVRKDGPCEGREKD 60

Query: 61 DCLIRRTLEAHTDYIYSGDTKP 74
          DCLIRRTLEAHTDYIYSGDTKP
Sbjct: 61 DCLIRRTLEAHTDYIYSGDTKP 76

BLAST of Carg11895 vs. NCBI nr
Match: XP_022131649.1 (uncharacterized protein LOC111004777 [Momordica charantia])

HSP 1 Score: 76.3 bits (186), Expect = 1.3e-10
Identity = 51/82 (62.20%), Postives = 61/82 (74.39%), Query Frame = 0

Query: 1   MSK-LAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVI---VSEEVCKD----- 60
           MSK LAALF IAAL++MS++L SS+ARPITT +A S  IA+P     V E+ C +     
Sbjct: 22  MSKQLAALFAIAALLTMSVLL-SSQARPITTFSANSVEIAHPDSKDRVHEDGCDERAEDQ 81

Query: 61  DCLIRRTLEAHTDYIYSGDTKP 74
           DCL RRTLEAHTDYIYSG+TKP
Sbjct: 82  DCLNRRTLEAHTDYIYSGETKP 102

BLAST of Carg11895 vs. NCBI nr
Match: KAG6585714.1 (Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 68.2 bits (165), Expect = 3.4e-08
Identity = 46/82 (56.10%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSA---VIANPVIVS---EEVCK---- 60
          MSKL ALF++ ALV+MSL L SSEARPI T TATS     IA+ V+     ++VC+    
Sbjct: 1  MSKLVALFSVVALVTMSL-LSSSEARPIATSTATSLDLNHIAHQVVSEKGHDDVCEGGET 60

Query: 61 DDCLIRRTLEAHTDYIYSGDTK 73
           +CL +RT+EAHTDYIY+ DTK
Sbjct: 61 KECLAKRTVEAHTDYIYTDDTK 81

BLAST of Carg11895 vs. NCBI nr
Match: XP_022132249.1 (phytosulfokines-like [Momordica charantia])

HSP 1 Score: 65.5 bits (158), Expect = 2.2e-07
Identity = 45/81 (55.56%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPV---IVSEEVC-----KDD 60
          M+KLAALFTI ALV    +L SSEARP     ATS  I NPV    V+ ++C      +D
Sbjct: 1  MTKLAALFTIVALVLTMSLLTSSEARP---TIATSVKIVNPVPEETVNGDICDERNPDED 60

Query: 61 CLIRRTLEAHTDYIYSGDTKP 74
          CL RRTL AHTDYIY+ +TKP
Sbjct: 61 CLFRRTLTAHTDYIYT-ETKP 77

BLAST of Carg11895 vs. ExPASy Swiss-Prot
Match: Q9FS10 (Phytosulfokines OS=Asparagus officinalis OX=4686 GN=PSK PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-05
Identity = 32/76 (42.11%), Postives = 43/76 (56.58%), Query Frame = 0

Query: 2  SKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVIVSEEVC----KDDCLIRR 61
          SK   L  IA L S+SL   +  ARP+    +T +V   P  V +E C    +++CL+RR
Sbjct: 3  SKAITLLLIALLFSLSL---AQAARPLQPADSTKSVHVIPEKVHDEACEGVGEEECLMRR 62

Query: 62 TLEAHTDYIYSGDTKP 74
          TL AH DYIY+ D  P
Sbjct: 63 TLTAHVDYIYTQDHNP 75

BLAST of Carg11895 vs. ExPASy TrEMBL
Match: A0A6J1BQV2 (Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111004777 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 6.1e-11
Identity = 51/82 (62.20%), Postives = 61/82 (74.39%), Query Frame = 0

Query: 1   MSK-LAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPVI---VSEEVCKD----- 60
           MSK LAALF IAAL++MS++L SS+ARPITT +A S  IA+P     V E+ C +     
Sbjct: 22  MSKQLAALFAIAALLTMSVLL-SSQARPITTFSANSVEIAHPDSKDRVHEDGCDERAEDQ 81

Query: 61  DCLIRRTLEAHTDYIYSGDTKP 74
           DCL RRTLEAHTDYIYSG+TKP
Sbjct: 82  DCLNRRTLEAHTDYIYSGETKP 102

BLAST of Carg11895 vs. ExPASy TrEMBL
Match: A0A6J1BSJ0 (Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111005149 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-07
Identity = 45/81 (55.56%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 1  MSKLAALFTIAALVSMSLMLPSSEARPITTLTATSAVIANPV---IVSEEVC-----KDD 60
          M+KLAALFTI ALV    +L SSEARP     ATS  I NPV    V+ ++C      +D
Sbjct: 1  MTKLAALFTIVALVLTMSLLTSSEARP---TIATSVKIVNPVPEETVNGDICDERNPDED 60

Query: 61 CLIRRTLEAHTDYIYSGDTKP 74
          CL RRTL AHTDYIY+ +TKP
Sbjct: 61 CLFRRTLTAHTDYIYT-ETKP 77

BLAST of Carg11895 vs. ExPASy TrEMBL
Match: A0A5A7V550 (Phytosulfokine-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001000 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.4e-04
Identity = 41/85 (48.24%), Postives = 56/85 (65.88%), Query Frame = 0

Query: 3  KLAALFT-IAALVSMS--LMLPSSE-ARPITTLTATSAVIANPVIVS---------EEVC 62
          KLAALFT IAALV++   +++PSSE ARPI +L      I +  +V+         E + 
Sbjct: 4  KLAALFTIIAALVTIMSLIVIPSSEAARPIPSLFKPQNPIVSRELVADHNNEGISCEGLG 63

Query: 63 KDDCLIRRTLEAHTDYIYSGD-TKP 74
          K+DCLI+RTL+AH DY+Y+ D TKP
Sbjct: 64 KEDCLIKRTLQAHIDYVYTCDPTKP 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6598323.16.0e-29100.00Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029293.1 Ph... [more]
XP_023546620.11.1e-1776.83phytosulfokines-like [Cucurbita pepo subsp. pepo][more]
XP_022131649.11.3e-1062.20uncharacterized protein LOC111004777 [Momordica charantia][more]
KAG6585714.13.4e-0856.10Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022132249.12.2e-0755.56phytosulfokines-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9FS102.2e-0542.11Phytosulfokines OS=Asparagus officinalis OX=4686 GN=PSK PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BQV26.1e-1162.20Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111004777 PE=3 SV=1[more]
A0A6J1BSJ01.1e-0755.56Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111005149 PE=3 SV=1[more]
A0A5A7V5509.4e-0448.24Phytosulfokine-beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009438PhytosulfokinePFAMPF06404PSKcoord: 48..70
e-value: 5.8E-7
score: 30.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11895-RACarg11895-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008283 cell population proliferation
cellular_component GO:0005576 extracellular region
cellular_component GO:0016020 membrane
molecular_function GO:0008083 growth factor activity