Carg11874 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11874
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionsubtilisin-like protease SBT1.7
LocationCarg_Chr05: 659431 .. 661752 (+)
RNA-Seq ExpressionCarg11874
SyntenyCarg11874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATTCAAACTTGCAGAGTTTCGCAATGGTTTCTTCTGTTTTTGATTTCGTCCTGTTCCTGCTCGTTTGCAGCAGCACAGAAGAGCAGTCAGCAGTTGAAGAAGAAGACTTATATAATTCATATGGACAGAACCAATATGCCACAAGCCTTTGATGATCATTTCCAGTGGTATGATTCTTCTTTGAAGTCAGTTTCTGATTCAGCCCAAACGCTTTATTACTACAACACAGTGGTTCATGGCTTCTCCACAAGATTGACAGTGGAGGAGGCTAAGCTGATAGAGAAGCAAGAAGGAATTCTGGCTGTCATACCGGAACTGAAATACGACCTCCATACCACACGGACGCCCGAGTTTCTTGGACTTGAGAAGAGCGTCTCTTTCTTCCCGGCGTCGGTGAAGGTCGGCGAAGTGATCGTCGGTGTTCTTGACACCGGTGTATGCCCTGAATTGGAGAGCTTCGATGATACAGGGCTAGGGCCGGTGCCTGTAAGCTGGAAAGGGGAGTGCGAAGTGGGTAAAAACTTCAGCTCTTCTAGTTGTAACAGGAAATTGATCGGAGCCAGGTACTTTTCAAAGGGCTATGAAGCGGCATTTGGTGCAATTGACGAATCCCAAGAGTCCAAATCGCCGAGGGATGACGATGGCCACGGAAGTCACACTTCAACAACCGCTGCTGGATCTGCCGTGACCGGAGCAAGCCTCTTTGGTTTCGCTGCAGGAACTGCAAAAGGTATGGCGGCTGAAGCTCGGGTTGCAACATATAAGGTATGTTGGCTTGGAGGGTGTTTCGGCTCTGACATTTTGGCTGCCATGGATAAGGCCATTGAAGATGGTGTCAATGTTCTATCCTTGTCGCTTGGTGGATCTTCCCCTGATTACTACAGAGACAATGTTGCCATTGGCGCCTTCTCTGCTACGGCTCAGGGAGTTTTTGTGTCATGTTCTGCTGGAAATGGTGGGCCATCATCAGGTAGTTTGTCCAACGTTGCGCCGTGGATAACCACTGTTGGCGCTGGGACTCTGGACAGAGACTTCCCTGTATATGTTACTCTTGGAAATGGGAAGAAGTTCACAGGGCAGTCGCTCTACAATGGAAAGCCCTTGTCAGACTCTTTAATACCAATTGTATATGCAGCCCATGCGAGTAATTCAACCAGTGGCAGCCTTTGTTTGACCAGTACTCTGAATCCGGCGAAGGTGGCCGGAAAAATAGTCGTCTGTGACAGAGGAGGGAACTCTCGAGTTCAGAAAGGGTTGGTGGTAAAAGAAGCCGGCGGTGCAGGGATGATTCTGGCCAACACTGAAACATACGGCGAGGAACAATTAGCCGATGCACATCTCTTGCCGGCGGCGGCCGTTGGCCAGAAAACGGGCGACGCAATAAAGAGCTACATCTCCACCAACGCTAATCCAACAGCAACAATCAGCGCCGGCACCACAAGATTGGGAGTTCAACCATCGCCGTTGGTGGCCGCATTCAGTTCTCGAGGCCCGAATCTCCTTACTCCGCAGATTCTCAAACCCGATCTAATAGCACCGGGAGTGAACATTCTGGCTGGATGGGCCGGCGGCGTCGGACCAACAGGCTTAGATAGCGATAAGCGACATGTGGCCTTCAACATCATCTCTGGCACATCAATGTCGTGCCCTCACATTAGCGGATTAGCCGCTCTTCTAAAAGCCGCTCATCCAGATTGGAGCCCAGCCGCCATTAGATCTGCTCTAATGACCACAGCTTACTCAACATACAAAACCGGCGAAGCAATACAAGACGTATCCAGCGGATTACCATCGACGCCGTTCGATATCGGAGCCGGACATGTGAATCCAACGGCCGCCCTCGATCCCGGCCTCGTTTACGATGCCACCATTGAGGACTACTTCGCCTTCCTCTGCGCCCTAAACTACAGCTCATTTCAAATCAAAGTAATCAGCAAGAAAGACTTCACCTGCAGTGGGAACAAGAACTACAAATTGGAGGATCTCAACTACCCGTCATTTGCTGTTGCATTGGAGACCCCTTCCACCAAAGGCGGCGGCGAAGGCACCGCACCAACCACCGTAAAATATACAAGGACTCTGACCAACAAGGGTGCCCCATCGACGTATAAGGTCTCTGTAACGTCGAAAAGCCCGTCGGTGAAGATTATGGTTGAACCAGAATCATTGAGTTTTGCGAAGCCCAATGAGCAGAAGAGTTACACGGTAACATTCGTTGCTTCTGCAATGCCGTCGGGGTCTGAGAGCTTTGCTCGTCTGGAATGGTCGGATGGGAAACACAATGTGGGTAGCCCCATTGCCTTCACCTGGACTTGA

mRNA sequence

ATGAAGATTCAAACTTGCAGAGTTTCGCAATGGTTTCTTCTGTTTTTGATTTCGTCCTGTTCCTGCTCGTTTGCAGCAGCACAGAAGAGCAGTCAGCAGTTGAAGAAGAAGACTTATATAATTCATATGGACAGAACCAATATGCCACAAGCCTTTGATGATCATTTCCAGTGGTATGATTCTTCTTTGAAGTCAGTTTCTGATTCAGCCCAAACGCTTTATTACTACAACACAGTGGTTCATGGCTTCTCCACAAGATTGACAGTGGAGGAGGCTAAGCTGATAGAGAAGCAAGAAGGAATTCTGGCTGTCATACCGGAACTGAAATACGACCTCCATACCACACGGACGCCCGAGTTTCTTGGACTTGAGAAGAGCGTCTCTTTCTTCCCGGCGTCGGTGAAGGTCGGCGAAGTGATCGTCGGTGTTCTTGACACCGGTGTATGCCCTGAATTGGAGAGCTTCGATGATACAGGGCTAGGGCCGGTGCCTGTAAGCTGGAAAGGGGAGTGCGAAGTGGGTAAAAACTTCAGCTCTTCTAGTTGTAACAGGAAATTGATCGGAGCCAGGTACTTTTCAAAGGGCTATGAAGCGGCATTTGGTGCAATTGACGAATCCCAAGAGTCCAAATCGCCGAGGGATGACGATGGCCACGGAAGTCACACTTCAACAACCGCTGCTGGATCTGCCGTGACCGGAGCAAGCCTCTTTGGTTTCGCTGCAGGAACTGCAAAAGGTATGGCGGCTGAAGCTCGGGTTGCAACATATAAGGTATGTTGGCTTGGAGGGTGTTTCGGCTCTGACATTTTGGCTGCCATGGATAAGGCCATTGAAGATGGTGTCAATGTTCTATCCTTGTCGCTTGGTGGATCTTCCCCTGATTACTACAGAGACAATGTTGCCATTGGCGCCTTCTCTGCTACGGCTCAGGGAGTTTTTGTGTCATGTTCTGCTGGAAATGGTGGGCCATCATCAGGTAGTTTGTCCAACGTTGCGCCGTGGATAACCACTGTTGGCGCTGGGACTCTGGACAGAGACTTCCCTGTATATGTTACTCTTGGAAATGGGAAGAAGTTCACAGGGCAGTCGCTCTACAATGGAAAGCCCTTGTCAGACTCTTTAATACCAATTGTATATGCAGCCCATGCGAGTAATTCAACCAGTGGCAGCCTTTGTTTGACCAGTACTCTGAATCCGGCGAAGGTGGCCGGAAAAATAGTCGTCTGTGACAGAGGAGGGAACTCTCGAGTTCAGAAAGGGTTGGTGGTAAAAGAAGCCGGCGGTGCAGGGATGATTCTGGCCAACACTGAAACATACGGCGAGGAACAATTAGCCGATGCACATCTCTTGCCGGCGGCGGCCGTTGGCCAGAAAACGGGCGACGCAATAAAGAGCTACATCTCCACCAACGCTAATCCAACAGCAACAATCAGCGCCGGCACCACAAGATTGGGAGTTCAACCATCGCCGTTGGTGGCCGCATTCAGTTCTCGAGGCCCGAATCTCCTTACTCCGCAGATTCTCAAACCCGATCTAATAGCACCGGGAGTGAACATTCTGGCTGGATGGGCCGGCGGCGTCGGACCAACAGGCTTAGATAGCGATAAGCGACATGTGGCCTTCAACATCATCTCTGGCACATCAATGTCGTGCCCTCACATTAGCGGATTAGCCGCTCTTCTAAAAGCCGCTCATCCAGATTGGAGCCCAGCCGCCATTAGATCTGCTCTAATGACCACAGCTTACTCAACATACAAAACCGGCGAAGCAATACAAGACGTATCCAGCGGATTACCATCGACGCCGTTCGATATCGGAGCCGGACATGTGAATCCAACGGCCGCCCTCGATCCCGGCCTCGTTTACGATGCCACCATTGAGGACTACTTCGCCTTCCTCTGCGCCCTAAACTACAGCTCATTTCAAATCAAAGTAATCAGCAAGAAAGACTTCACCTGCAGTGGGAACAAGAACTACAAATTGGAGGATCTCAACTACCCGTCATTTGCTGTTGCATTGGAGACCCCTTCCACCAAAGGCGGCGGCGAAGGCACCGCACCAACCACCGTAAAATATACAAGGACTCTGACCAACAAGGGTGCCCCATCGACGTATAAGGTCTCTGTAACGTCGAAAAGCCCGTCGGTGAAGATTATGGTTGAACCAGAATCATTGAGTTTTGCGAAGCCCAATGAGCAGAAGAGTTACACGGTAACATTCGTTGCTTCTGCAATGCCGTCGGGGTCTGAGAGCTTTGCTCGTCTGGAATGGTCGGATGGGAAACACAATGTGGGTAGCCCCATTGCCTTCACCTGGACTTGA

Coding sequence (CDS)

ATGAAGATTCAAACTTGCAGAGTTTCGCAATGGTTTCTTCTGTTTTTGATTTCGTCCTGTTCCTGCTCGTTTGCAGCAGCACAGAAGAGCAGTCAGCAGTTGAAGAAGAAGACTTATATAATTCATATGGACAGAACCAATATGCCACAAGCCTTTGATGATCATTTCCAGTGGTATGATTCTTCTTTGAAGTCAGTTTCTGATTCAGCCCAAACGCTTTATTACTACAACACAGTGGTTCATGGCTTCTCCACAAGATTGACAGTGGAGGAGGCTAAGCTGATAGAGAAGCAAGAAGGAATTCTGGCTGTCATACCGGAACTGAAATACGACCTCCATACCACACGGACGCCCGAGTTTCTTGGACTTGAGAAGAGCGTCTCTTTCTTCCCGGCGTCGGTGAAGGTCGGCGAAGTGATCGTCGGTGTTCTTGACACCGGTGTATGCCCTGAATTGGAGAGCTTCGATGATACAGGGCTAGGGCCGGTGCCTGTAAGCTGGAAAGGGGAGTGCGAAGTGGGTAAAAACTTCAGCTCTTCTAGTTGTAACAGGAAATTGATCGGAGCCAGGTACTTTTCAAAGGGCTATGAAGCGGCATTTGGTGCAATTGACGAATCCCAAGAGTCCAAATCGCCGAGGGATGACGATGGCCACGGAAGTCACACTTCAACAACCGCTGCTGGATCTGCCGTGACCGGAGCAAGCCTCTTTGGTTTCGCTGCAGGAACTGCAAAAGGTATGGCGGCTGAAGCTCGGGTTGCAACATATAAGGTATGTTGGCTTGGAGGGTGTTTCGGCTCTGACATTTTGGCTGCCATGGATAAGGCCATTGAAGATGGTGTCAATGTTCTATCCTTGTCGCTTGGTGGATCTTCCCCTGATTACTACAGAGACAATGTTGCCATTGGCGCCTTCTCTGCTACGGCTCAGGGAGTTTTTGTGTCATGTTCTGCTGGAAATGGTGGGCCATCATCAGGTAGTTTGTCCAACGTTGCGCCGTGGATAACCACTGTTGGCGCTGGGACTCTGGACAGAGACTTCCCTGTATATGTTACTCTTGGAAATGGGAAGAAGTTCACAGGGCAGTCGCTCTACAATGGAAAGCCCTTGTCAGACTCTTTAATACCAATTGTATATGCAGCCCATGCGAGTAATTCAACCAGTGGCAGCCTTTGTTTGACCAGTACTCTGAATCCGGCGAAGGTGGCCGGAAAAATAGTCGTCTGTGACAGAGGAGGGAACTCTCGAGTTCAGAAAGGGTTGGTGGTAAAAGAAGCCGGCGGTGCAGGGATGATTCTGGCCAACACTGAAACATACGGCGAGGAACAATTAGCCGATGCACATCTCTTGCCGGCGGCGGCCGTTGGCCAGAAAACGGGCGACGCAATAAAGAGCTACATCTCCACCAACGCTAATCCAACAGCAACAATCAGCGCCGGCACCACAAGATTGGGAGTTCAACCATCGCCGTTGGTGGCCGCATTCAGTTCTCGAGGCCCGAATCTCCTTACTCCGCAGATTCTCAAACCCGATCTAATAGCACCGGGAGTGAACATTCTGGCTGGATGGGCCGGCGGCGTCGGACCAACAGGCTTAGATAGCGATAAGCGACATGTGGCCTTCAACATCATCTCTGGCACATCAATGTCGTGCCCTCACATTAGCGGATTAGCCGCTCTTCTAAAAGCCGCTCATCCAGATTGGAGCCCAGCCGCCATTAGATCTGCTCTAATGACCACAGCTTACTCAACATACAAAACCGGCGAAGCAATACAAGACGTATCCAGCGGATTACCATCGACGCCGTTCGATATCGGAGCCGGACATGTGAATCCAACGGCCGCCCTCGATCCCGGCCTCGTTTACGATGCCACCATTGAGGACTACTTCGCCTTCCTCTGCGCCCTAAACTACAGCTCATTTCAAATCAAAGTAATCAGCAAGAAAGACTTCACCTGCAGTGGGAACAAGAACTACAAATTGGAGGATCTCAACTACCCGTCATTTGCTGTTGCATTGGAGACCCCTTCCACCAAAGGCGGCGGCGAAGGCACCGCACCAACCACCGTAAAATATACAAGGACTCTGACCAACAAGGGTGCCCCATCGACGTATAAGGTCTCTGTAACGTCGAAAAGCCCGTCGGTGAAGATTATGGTTGAACCAGAATCATTGAGTTTTGCGAAGCCCAATGAGCAGAAGAGTTACACGGTAACATTCGTTGCTTCTGCAATGCCGTCGGGGTCTGAGAGCTTTGCTCGTCTGGAATGGTCGGATGGGAAACACAATGTGGGTAGCCCCATTGCCTTCACCTGGACTTGA

Protein sequence

MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Homology
BLAST of Carg11874 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1518.4 bits (3930), Expect = 0.0e+00
Identity = 773/773 (100.00%), Postives = 773/773 (100.00%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
           AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Carg11874 vs. NCBI nr
Match: KAG6598344.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1515.4 bits (3922), Expect = 0.0e+00
Identity = 772/773 (99.87%), Postives = 772/773 (99.87%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
           AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG
Sbjct: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA
Sbjct: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCS NKNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSRNKNYK 660

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV
Sbjct: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Carg11874 vs. NCBI nr
Match: XP_022961887.1 (subtilisin-like protease SBT1.7 [Cucurbita moschata])

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 767/773 (99.22%), Postives = 769/773 (99.48%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFG 240

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
           AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           LVVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG
Sbjct: 421 LVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           +TRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGW  GVGPTGLDSDKRHVA
Sbjct: 481 STRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVA 540

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAVALETPSTKGG EGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV
Sbjct: 661 LEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Carg11874 vs. NCBI nr
Match: XP_023545694.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1499.6 bits (3881), Expect = 0.0e+00
Identity = 761/773 (98.45%), Postives = 766/773 (99.09%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MKIQTCRVSQWFLLFLISSCSCSF AAQKSSQQ+KK+TYIIHMDRTNMPQ FDDHFQWYD
Sbjct: 38  MKIQTCRVSQWFLLFLISSCSCSFVAAQKSSQQVKKQTYIIHMDRTNMPQVFDDHFQWYD 97

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQ+GILAVIPELKYDLHTTRTPEF
Sbjct: 98  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQQGILAVIPELKYDLHTTRTPEF 157

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNF+SS
Sbjct: 158 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFTSS 217

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA
Sbjct: 218 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 277

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
            GTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 278 EGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 337

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT
Sbjct: 338 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 397

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 398 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 457

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           LVVK+AGGAGMILANTETYGEEQLADAHLLPA AVGQKTGDAIKSYISTNANPTATISAG
Sbjct: 458 LVVKDAGGAGMILANTETYGEEQLADAHLLPAVAVGQKTGDAIKSYISTNANPTATISAG 517

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGW  GVGPTGLDSDKRHVA
Sbjct: 518 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVA 577

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS
Sbjct: 578 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 637

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK
Sbjct: 638 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 697

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAVALETPSTKGG EGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV
Sbjct: 698 LEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 757

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 758 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 810

BLAST of Carg11874 vs. NCBI nr
Match: XP_022996498.1 (subtilisin-like protease SBT1.7 [Cucurbita maxima])

HSP 1 Score: 1480.7 bits (3832), Expect = 0.0e+00
Identity = 758/775 (97.81%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLIS--SCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQW 60
           MKIQTCRVSQWFLLFLIS  SCSCSFAAAQ      KKKTYIIHMDRTNMPQAFDDHFQW
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSCSFAAAQ------KKKTYIIHMDRTNMPQAFDDHFQW 60

Query: 61  YDSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTP 120
           YDSSLKSVSDSAQTLYYYNTV HGFSTRLTVEEAKLIEKQ+GILAVIPELKYDLHTTRTP
Sbjct: 61  YDSSLKSVSDSAQTLYYYNTVAHGFSTRLTVEEAKLIEKQQGILAVIPELKYDLHTTRTP 120

Query: 121 EFLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFS 180
           EFLGLEKSVSFFPASVKVGEVIVGVLDTGV PELESFDDTGLGPVPVSWKGECEVGKNF+
Sbjct: 121 EFLGLEKSVSFFPASVKVGEVIVGVLDTGVWPELESFDDTGLGPVPVSWKGECEVGKNFT 180

Query: 181 SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG 240
           SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG
Sbjct: 181 SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG 240

Query: 241 FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD 300
           FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD
Sbjct: 241 FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD 300

Query: 301 NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK 360
           NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK
Sbjct: 301 NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK 360

Query: 361 FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ 420
           FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ
Sbjct: 361 FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ 420

Query: 421 KGLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATIS 480
           KGLVVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYIS++ANPTATIS
Sbjct: 421 KGLVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISSDANPTATIS 480

Query: 481 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRH 540
           AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGW GGVGPTGLDSDKRH
Sbjct: 481 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGVGPTGLDSDKRH 540

Query: 541 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGL 600
           VAFNIISGTSMSCPHISGLAALLKAAHPDWSPA IRSALMTTAYSTYKTGEAIQDVSSGL
Sbjct: 541 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAVIRSALMTTAYSTYKTGEAIQDVSSGL 600

Query: 601 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 660
           PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN
Sbjct: 601 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 660

Query: 661 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI 720
           YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI
Sbjct: 661 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI 720

Query: 721 MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 769

BLAST of Carg11874 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 1.8e-280
Identity = 488/765 (63.79%), Postives = 590/765 (77.12%), Query Frame = 0

Query: 11  WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSA 70
           +FLL  +  C  S +++ +        TYI+HM ++ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGFCHVSSSSSDQG-------TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 71  QTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGL-EKSVSF 130
           + LY Y   +HGFSTRLT EEA  +  Q G+++V+PE +Y+LHTTRTP FLGL E +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 131 FPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGA 190
           FP +    +V+VGVLDTGV PE +S+ D G GP+P SWKG CE G NF++S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 191 RYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGMAA 250
           R+F++GYE+  G IDES+ES+SPRDDDGHG+HTS+TAAGS V GASL G+A+GTA+GMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 251 EARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSATA 310
            ARVA YKVCWLGGCF SDILAA+DKAI D VNVLS+SLGG   DYYRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 311 QGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNGKP 370
           +G+ VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFP    LGNGK FTG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 371 LSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKEAGGA 430
           L D L+P +YA +ASN+T+G+LC+T TL P KV GKIV+CDRG N+RVQKG VVK AGG 
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 431 GMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTRLGVQPS 490
           GMILANT   GEE +ADAHLLPA  VG+K GD I+ Y++T+ NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 491 PLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNIISGTSM 550
           P+VAAFSSRGPN +TP ILKPDLIAPGVNILA W G  GPTGL SD R V FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 551 SCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPFDIGAGH 610
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTTAY TYK G+ + D+++G PSTPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 611 VNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLEDLNYPSF 670
           V+PT A +PGL+YD T EDY  FLCALNY+S QI+ +S++++TC  +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 671 AVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPESLSFAK 730
           AV ++       G G      KYTRT+T+ G   TY V VTS++  VKI VEP  L+F +
Sbjct: 671 AVNVD-------GVG----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 730

Query: 731 PNEQKSYTVTF-VASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
            NE+KSYTVTF V S+ PSGS SF  +EWSDGKH VGSP+A +WT
Sbjct: 731 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Carg11874 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 2.4e-219
Identity = 413/742 (55.66%), Postives = 507/742 (68.33%), Query Frame = 0

Query: 36  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEA-KL 95
           KKTYII ++ ++ P++F  H  WY S L S S     LY Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 96  IEKQEGILAVIPELKYDLHTTRTPEFLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELES 155
           +     IL +  +  Y LHTTRTPEFLGL               VI+GVLDTGV PE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 156 FDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGARYFSKGYE-AAFGAIDESQESKSPR 215
           FDDT +  +P  WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 216 DDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAM 275
           D DGHG+HTSTTAAGSAV  AS  G+AAGTA+GMA  ARVATYKVCW  GCFGSDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 276 DKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAP 335
           D+AI DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 336 WITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCL 395
           W+ TVGAGTLDRDFP +  LGNGK+ TG SLY+G  +    + +VY  +  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 396 TSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKEAGGAGMILANTETYGEEQLADAHLLPAA 455
             +L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 456 AVGQKTGDAIKSYISTNANPTATISAGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLI 515
           AVG+KTGD ++ Y+ +++ PTA +    T L V+PSP+VAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 516 APGVNILAGWAGGVGPTGLDSDKRHVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 575
            PGVNILAGW+  +GPTGLD D R   FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 576 RSALMTTAYSTYKTGEAIQDVSSGLPSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFL 635
           +SALMTTAY    T   + D +    S P+  G+GHV+P  AL PGLVYD + E+Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 636 CALNYSSFQIKVISKKDFTCSGNKNYKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYT 695
           C+L+Y+   I  I K+       K      LNYPSF+V         GG+      V+YT
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GGK----RVVRYT 686

Query: 696 RTLTNKGAPST-YKVSVTSKSPSVKIMVEPESLSFAKPNEQKSYTVTFVA--SAMPSGSE 755
           R +TN GA S+ YKV+V   +PSV I V+P  LSF    E+K YTVTFV+      +   
Sbjct: 687 REVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKA 746

Query: 756 SFARLEWSDGKHNVGSPIAFTW 773
            F  + WS+ +H V SP+AF+W
Sbjct: 747 EFGSITWSNPQHEVRSPVAFSW 751

BLAST of Carg11874 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 1.2e-215
Identity = 403/775 (52.00%), Postives = 520/775 (67.10%), Query Frame = 0

Query: 14  LFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 73
           LF+I S +  F  A+ ++Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 74  ---DSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLE 133
              ++ + LY Y T  HG + +LT EEA+ +E+++G++AVIPE +Y+LHTTR+P FLGLE
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 134 KSVS--FFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 193
           +  S   +   V   +V+VGVLDTG+ PE ESF+DTG+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 194 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 253
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHG+HT+ T AGS V GA+LFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 254 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 313
           TA+GMA +ARVA YKVCW+GGCF SDIL+A+D+A+ DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 314 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 373
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFP  V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 374 SLYNGKPL--SDSLIPIVY-AAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 433
           SLY G+ +   +   P+VY   +AS+    S CL   L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 434 GLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 493
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  T+   TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 494 GTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHV 553
             TR+G++PSP+VAAFSSRGPN L+ +ILKPDL+APGVNILA W G + P+ L SD R V
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 554 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 613
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 614 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISK-KDFTCSGNKN 673
           S+P+D GAGH++P  A DPGLVYD   ++YF FLC  + S  Q+KV +K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 674 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAP-STYKVSVTSKSPSVK 733
               +LNYP+ +            E T    +   RT+TN G   S+YKVSV S      
Sbjct: 672 KNPGNLNYPAISALFP--------ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGAS 731

Query: 734 IMVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTW 773
           + V+P++L+F   +++ SYTVTF         E F  L W    H V SP+  TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE-FGGLVWKSTTHKVRSPVIITW 776

BLAST of Carg11874 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.4e-206
Identity = 398/778 (51.16%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 13  LLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQ- 72
           + F+     C F+ +  SS  L  ++YI+H+ R++ P  F  H  W+ S L+S+  S Q 
Sbjct: 9   IFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 68

Query: 73  --TLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLEKSVSF 132
              LY Y+  VHGFS RL+  +   + +   +++VIP+   ++HTT TP FLG  ++   
Sbjct: 69  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 128

Query: 133 FPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGA 192
           +  S    +VIVGVLDTG+ PE  SF D+GLGP+P +WKGECE+G +F +SSCNRKLIGA
Sbjct: 129 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 188

Query: 193 RYFSKGYEAAFGAIDE--SQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGM 252
           R F +GY        +  ++ES+SPRD +GHG+HT++TAAGS V  ASL+ +A GTA GM
Sbjct: 189 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 248

Query: 253 AAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLG--GSSPDYYRDNVAIGAF 312
           A++AR+A YK+CW GGC+ SDILAAMD+A+ DGV+V+SLS+G  GS+P+Y+ D++AIGAF
Sbjct: 249 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 308

Query: 313 SATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLY 372
            AT  G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F      G+GK FTG SLY
Sbjct: 309 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 368

Query: 373 NGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKE 432
            G+ L DS + +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG  VK 
Sbjct: 369 AGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 428

Query: 433 AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTRLG 492
           AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI T+ +PTA IS   T +G
Sbjct: 429 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 488

Query: 493 VQ-PSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNII 552
              PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGW G VGPT LD D R V FNII
Sbjct: 489 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 548

Query: 553 SGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPFD 612
           SGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE I+D+++G  S  F 
Sbjct: 549 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 608

Query: 613 IGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDF---TCSGNKNYKL 672
            GAGHV+P  AL+PGLVYD  +++Y AFLCA+ Y    I V  +       C  +K    
Sbjct: 609 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 668

Query: 673 EDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGA--PSTYKVSVTSKSPSVKIM 732
            DLNYPSF+V   +      GE      VKY R + N G+   + Y+V V S + +V+I 
Sbjct: 669 GDLNYPSFSVVFAST-----GE-----VVKYKRVVKNVGSNVDAVYEVGVKSPA-NVEID 728

Query: 733 VEPESLSFAKPNEQKSYTVTFVASAMPSG-----SESFARLEWSDGKHNVGSPIAFTW 773
           V P  L+F+K      Y VTF +  +  G        F  +EW+DG+H V SP+A  W
Sbjct: 729 VSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Carg11874 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 2.1e-199
Identity = 382/779 (49.04%), Postives = 512/779 (65.73%), Query Frame = 0

Query: 11  WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSA 70
           ++  FL++  S S +A+  +S      TYI+H+D    P  F  HF WY SSL S++ S 
Sbjct: 5   FYFFFLLTLSSPSSSASSSNS-----LTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 64

Query: 71  QTLYY-YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLEKS--V 130
            ++ + Y+TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +   
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 131 SFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLI 190
                S    ++++GV+DTGV PE  SFDD GLGPVP+ WKG+C   ++F  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 191 GARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGM 250
           GAR+F  GYEA  G ++E+ E +SPRD DGHG+HT++ +AG  V  AS  G+A G A GM
Sbjct: 185 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 244

Query: 251 AAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSA 310
           A +AR+A YKVCW  GC+ SDILAA D A+ DGV+V+SLS+GG    YY D +AIGAF A
Sbjct: 245 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 304

Query: 311 TAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNG 370
             +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFP  V LGNGK  +G S+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 KPLSDS-LIPIVYAAH--ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVK 430
             L    + P+VY       +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 EAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYIS------TNANPTATIS 490
           + GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      ++ +PTATI 
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRH 550
              TRLG++P+P+VA+FS+RGPN  TP+ILKPD+IAPG+NILA W   +GP+G+ SD R 
Sbjct: 485 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 544

Query: 551 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGL 610
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G 
Sbjct: 545 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 604

Query: 611 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 670
            S+  D G+GHV+PT A+DPGLVYD T  DY  FLC  NY+   I  I+++   C G + 
Sbjct: 605 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 664

Query: 671 Y-KLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKG-APSTYKVSVTSKSPSV 730
              + +LNYPSF+V  +         G +  +  + RT+TN G + S Y++ +       
Sbjct: 665 AGHVGNLNYPSFSVVFQQ-------YGESKMSTHFIRTVTNVGDSDSVYEIKIRPPR-GT 724

Query: 731 KIMVEPESLSFAKPNEQKSYTV----TFVASAMPSGSESFARLEWSDGKHNVGSPIAFT 772
            + VEPE LSF +  ++ S+ V    T V  +  + +     + WSDGK NV SP+  T
Sbjct: 725 TVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Carg11874 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 767/773 (99.22%), Postives = 769/773 (99.48%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF
Sbjct: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS
Sbjct: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGF 
Sbjct: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFG 240

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
           AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT
Sbjct: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           LVVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG
Sbjct: 421 LVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           +TRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGW  GVGPTGLDSDKRHVA
Sbjct: 481 STRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVA 540

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS
Sbjct: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK
Sbjct: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAVALETPSTKGG EGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV
Sbjct: 661 LEDLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of Carg11874 vs. ExPASy TrEMBL
Match: A0A6J1KAX6 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111491728 PE=3 SV=1)

HSP 1 Score: 1480.7 bits (3832), Expect = 0.0e+00
Identity = 758/775 (97.81%), Postives = 763/775 (98.45%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLIS--SCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQW 60
           MKIQTCRVSQWFLLFLIS  SCSCSFAAAQ      KKKTYIIHMDRTNMPQAFDDHFQW
Sbjct: 1   MKIQTCRVSQWFLLFLISSCSCSCSFAAAQ------KKKTYIIHMDRTNMPQAFDDHFQW 60

Query: 61  YDSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTP 120
           YDSSLKSVSDSAQTLYYYNTV HGFSTRLTVEEAKLIEKQ+GILAVIPELKYDLHTTRTP
Sbjct: 61  YDSSLKSVSDSAQTLYYYNTVAHGFSTRLTVEEAKLIEKQQGILAVIPELKYDLHTTRTP 120

Query: 121 EFLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFS 180
           EFLGLEKSVSFFPASVKVGEVIVGVLDTGV PELESFDDTGLGPVPVSWKGECEVGKNF+
Sbjct: 121 EFLGLEKSVSFFPASVKVGEVIVGVLDTGVWPELESFDDTGLGPVPVSWKGECEVGKNFT 180

Query: 181 SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG 240
           SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG
Sbjct: 181 SSSCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFG 240

Query: 241 FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD 300
           FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD
Sbjct: 241 FAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRD 300

Query: 301 NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK 360
           NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK
Sbjct: 301 NVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKK 360

Query: 361 FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ 420
           FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ
Sbjct: 361 FTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQ 420

Query: 421 KGLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATIS 480
           KGLVVK+AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYIS++ANPTATIS
Sbjct: 421 KGLVVKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISSDANPTATIS 480

Query: 481 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRH 540
           AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGW GGVGPTGLDSDKRH
Sbjct: 481 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGVGPTGLDSDKRH 540

Query: 541 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGL 600
           VAFNIISGTSMSCPHISGLAALLKAAHPDWSPA IRSALMTTAYSTYKTGEAIQDVSSGL
Sbjct: 541 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAVIRSALMTTAYSTYKTGEAIQDVSSGL 600

Query: 601 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 660
           PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN
Sbjct: 601 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 660

Query: 661 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI 720
           YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI
Sbjct: 661 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKI 720

Query: 721 MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT
Sbjct: 721 MVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 769

BLAST of Carg11874 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 677/771 (87.81%), Postives = 723/771 (93.77%), Query Frame = 0

Query: 3   IQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 62
           ++TCRVSQWFLLFLIS CSCSF  AQKS+QQLKKKTYIIHMD+TNMPQAFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 63  LKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLG 122
           LKSVSDSAQ LY YNTV+HGFSTRLTVEEAKL+EKQEGI+AVIPE+KY+LHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 123 LEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 182
           L KSVSFFPAS KV EVI+GVLDTGV PELESF D GLGP+P SWKGECEVGKNF+SS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 183 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 242
           NRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHGSHTSTTAAGSAVTGA+LFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 243 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 302
           TA+GMAAEARVATYKVCWLGGCF SDILAAMDK++EDG N+LS+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 303 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 362
           GAFSATAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFP YVTLGNGKK TG+
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 363 SLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLV 422
           SLY+GKPL +SL+PIV AA ASNS+SGSLCL+ TLNPAKV GKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 423 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTT 482
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQK GDAIK+YIS+++NPTATIS GTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 483 RLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFN 542
           RLGVQPSP+VAAFSSRGPNLLTPQILKPDLIAPGVNILAGW GG GPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 543 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTP 602
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GE IQD+S+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 603 FDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLE 662
           FDIGAGHVNPTAALDPGLVYD T +DY AFLCALNYSS QIKVISKKDFTC+GNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 663 DLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEP 722
           DLNYPSFAV LETPST+ GGE  APTT+KYTRTLTNKGA STYKVSVT+KS SVKI+VEP
Sbjct: 661 DLNYPSFAVPLETPSTR-GGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEP 720

Query: 723 ESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           ESLSF + NEQKSYTVTF+AS MPSGS+SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 ESLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Carg11874 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 678/773 (87.71%), Postives = 726/773 (93.92%), Query Frame = 0

Query: 1   MKIQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYD 60
           MK+QTCRVS+ FLLFLI   S S+  A+K++Q+LKKKTYIIHMDRTNMPQAFDDHFQWYD
Sbjct: 4   MKMQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYD 63

Query: 61  SSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEF 120
           SSLKSVSDSAQ LY Y+TV+HGFSTRLTVEEA+L+EK+EGILAVIPE KY+LHTTRTPEF
Sbjct: 64  SSLKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEF 123

Query: 121 LGLEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSS 180
           LGL KS SFFPASVKVGEVI+G+LDTGV PELESF+D GLGPVP SWKGECEVGKNFSSS
Sbjct: 124 LGLGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSS 183

Query: 181 SCNRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFA 240
           SCNRKLIGARYF+KGYE+AFG IDESQESKSP+DDDGHG+HTSTTAAGSAVTGA+LFG+A
Sbjct: 184 SCNRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYA 243

Query: 241 AGTAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNV 300
           +GTA+GMAA+ARVATYKVCWLGGCFGSDILAA+DKA+EDGVNVLSLSLGGSSPDYYRDNV
Sbjct: 244 SGTARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNV 303

Query: 301 AIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFT 360
           AIGAFSA AQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFP Y+TLGNGKKFT
Sbjct: 304 AIGAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFT 363

Query: 361 GQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKG 420
           G+SLY+GKPL DSL+PIVYAAHASNSTSGS CLT TLNPAKVAGKIVVCDRGGNSRVQKG
Sbjct: 364 GESLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKG 423

Query: 421 LVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAG 480
           +VVKEAGGAGMILANTETYGEE+LADAHLLPAAAVGQKTGDAIKSYIS++ANPTA  S G
Sbjct: 424 VVVKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTG 483

Query: 481 TTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVA 540
           TTRLGVQPSP+VAAFSSRGPNLLTP ILKPDLIAPGVNILAGW G  GPTGLD DKR VA
Sbjct: 484 TTRLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVA 543

Query: 541 FNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPS 600
           FNIISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYK GEAIQD+SSGLPS
Sbjct: 544 FNIISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPS 603

Query: 601 TPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYK 660
           TPFDIGAGHVNP AALDPGLVYD T +DYF FLCALNY+S QIKVI+KKDFTCS +KNYK
Sbjct: 604 TPFDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYK 663

Query: 661 LEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMV 720
           LEDLNYPSFAV LETPS +GGG+  APTTVKYTRTLTNK A STYKVS TSKSPSVKI+V
Sbjct: 664 LEDLNYPSFAVPLETPSVRGGGD-AAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVV 723

Query: 721 EPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           EPESLSFA+ NEQKSYTVTF+AS MPSG+ESFARLEWSDGKH VGSPIAFTWT
Sbjct: 724 EPESLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of Carg11874 vs. ExPASy TrEMBL
Match: A0A1S3BA54 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=1)

HSP 1 Score: 1336.2 bits (3457), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 717/771 (93.00%), Query Frame = 0

Query: 3   IQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 62
           ++TCRVSQWFLLFLIS  SCSF  AQKS+QQLKKKTYIIHMD+T+MPQAFDDHFQWYD+S
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTS 60

Query: 63  LKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLG 122
           LKSVSDSAQ LY YN V+HGFST LTVEEAKL+EKQEGI+AV+PE+KY+LHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 123 LEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 182
           L KS SFFPAS KV EVI+G+LDTGV PELESF D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 183 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 242
           NRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHGSHTSTTAAGSAVTGA+LFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 243 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 302
           TA+GMAAEARVATYKVCWLGGCF SDILAA+DK++EDG N++S+SLGG+S DYYRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 303 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 362
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFP YVTLGNGKK TG+
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 363 SLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLV 422
           SLY+GKPL +SL+PIV AA ASNS+SGSLCL+ TLNPAKVAGKIVVCDRGGNSRVQKGLV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 423 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTT 482
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQ+ GDAIKSYIS+++NPTATIS GTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTT 480

Query: 483 RLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFN 542
           RLGVQPSP+VAAFSSRGPNLLTPQILKPDLIAPGVNILAGW GG GPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 543 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTP 602
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GE IQD+S+G PSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 603 FDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLE 662
           FDIGAGHVNPTAALDPGLVYD T +DY AFLCALNYSS QIKVISKKDFTC+GNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 663 DLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEP 722
           DLNYPSFAV LETPSTK  GE  APTTVKYTRTLTNKGAPSTYKVSVT+K  SVKI+V P
Sbjct: 661 DLNYPSFAVPLETPSTK-RGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAP 720

Query: 723 ESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
           ESLSF + NEQKSYTVTF+AS MPSGS+SFARLEWSDGKH VGSPIAFTWT
Sbjct: 721 ESLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of Carg11874 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 966.5 bits (2497), Expect = 1.3e-281
Identity = 488/765 (63.79%), Postives = 590/765 (77.12%), Query Frame = 0

Query: 11  WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSA 70
           +FLL  +  C  S +++ +        TYI+HM ++ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGFCHVSSSSSDQG-------TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 71  QTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGL-EKSVSF 130
           + LY Y   +HGFSTRLT EEA  +  Q G+++V+PE +Y+LHTTRTP FLGL E +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 131 FPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGA 190
           FP +    +V+VGVLDTGV PE +S+ D G GP+P SWKG CE G NF++S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 191 RYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGMAA 250
           R+F++GYE+  G IDES+ES+SPRDDDGHG+HTS+TAAGS V GASL G+A+GTA+GMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 251 EARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSATA 310
            ARVA YKVCWLGGCF SDILAA+DKAI D VNVLS+SLGG   DYYRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 311 QGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNGKP 370
           +G+ VSCSAGN GPSS SLSNVAPWITTVGAGTLDRDFP    LGNGK FTG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 371 LSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKEAGGA 430
           L D L+P +YA +ASN+T+G+LC+T TL P KV GKIV+CDRG N+RVQKG VVK AGG 
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 431 GMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTRLGVQPS 490
           GMILANT   GEE +ADAHLLPA  VG+K GD I+ Y++T+ NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 491 PLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNIISGTSM 550
           P+VAAFSSRGPN +TP ILKPDLIAPGVNILA W G  GPTGL SD R V FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 551 SCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPFDIGAGH 610
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTTAY TYK G+ + D+++G PSTPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 611 VNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLEDLNYPSF 670
           V+PT A +PGL+YD T EDY  FLCALNY+S QI+ +S++++TC  +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 671 AVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEPESLSFAK 730
           AV ++       G G      KYTRT+T+ G   TY V VTS++  VKI VEP  L+F +
Sbjct: 671 AVNVD-------GVG----AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 730

Query: 731 PNEQKSYTVTF-VASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 774
            NE+KSYTVTF V S+ PSGS SF  +EWSDGKH VGSP+A +WT
Sbjct: 731 ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Carg11874 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 763.5 bits (1970), Expect = 1.7e-220
Identity = 413/742 (55.66%), Postives = 507/742 (68.33%), Query Frame = 0

Query: 36  KKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQTLYYYNTVVHGFSTRLTVEEA-KL 95
           KKTYII ++ ++ P++F  H  WY S L S S     LY Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 96  IEKQEGILAVIPELKYDLHTTRTPEFLGLEKSVSFFPASVKVGEVIVGVLDTGVCPELES 155
           +     IL +  +  Y LHTTRTPEFLGL               VI+GVLDTGV PE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 156 FDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGARYFSKGYE-AAFGAIDESQESKSPR 215
           FDDT +  +P  WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 216 DDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGMAAEARVATYKVCWLGGCFGSDILAAM 275
           D DGHG+HTSTTAAGSAV  AS  G+AAGTA+GMA  ARVATYKVCW  GCFGSDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 276 DKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSATAQGVFVSCSAGNGGPSSGSLSNVAP 335
           D+AI DGV+VLSLSLGG S  YYRD +AIGAFSA  +GVFVSCSAGN GP+  S++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 336 WITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNGKPLSDSLIPIVYAAHASNSTSGSLCL 395
           W+ TVGAGTLDRDFP +  LGNGK+ TG SLY+G  +    + +VY  +  NS+S +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 396 TSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKEAGGAGMILANTETYGEEQLADAHLLPAA 455
             +L+ + V GKIVVCDRG N+RV+KG VV++AGG GMI+ANT   GEE +AD+HLLPA 
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 456 AVGQKTGDAIKSYISTNANPTATISAGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLI 515
           AVG+KTGD ++ Y+ +++ PTA +    T L V+PSP+VAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 516 APGVNILAGWAGGVGPTGLDSDKRHVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 575
            PGVNILAGW+  +GPTGLD D R   FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 576 RSALMTTAYSTYKTGEAIQDVSSGLPSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFL 635
           +SALMTTAY    T   + D +    S P+  G+GHV+P  AL PGLVYD + E+Y  FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 636 CALNYSSFQIKVISKKDFTCSGNKNYKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYT 695
           C+L+Y+   I  I K+       K      LNYPSF+V         GG+      V+YT
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF-------GGK----RVVRYT 686

Query: 696 RTLTNKGAPST-YKVSVTSKSPSVKIMVEPESLSFAKPNEQKSYTVTFVA--SAMPSGSE 755
           R +TN GA S+ YKV+V   +PSV I V+P  LSF    E+K YTVTFV+      +   
Sbjct: 687 REVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKA 746

Query: 756 SFARLEWSDGKHNVGSPIAFTW 773
            F  + WS+ +H V SP+AF+W
Sbjct: 747 EFGSITWSNPQHEVRSPVAFSW 751

BLAST of Carg11874 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 751.1 bits (1938), Expect = 8.7e-217
Identity = 403/775 (52.00%), Postives = 520/775 (67.10%), Query Frame = 0

Query: 14  LFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVS------ 73
           LF+I S +  F  A+ ++Q   KKTY+IHMD++ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 74  ---DSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLE 133
              ++ + LY Y T  HG + +LT EEA+ +E+++G++AVIPE +Y+LHTTR+P FLGLE
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 134 KSVS--FFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 193
           +  S   +   V   +V+VGVLDTG+ PE ESF+DTG+ PVP +W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 194 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 253
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHG+HT+ T AGS V GA+LFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 254 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 313
           TA+GMA +ARVA YKVCW+GGCF SDIL+A+D+A+ DGV VLS+SLGG    Y RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 314 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 373
             F A   GVFVSCSAGNGGP   SL+NV+PWITTVGA T+DRDFP  V +G  + F G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 374 SLYNGKPL--SDSLIPIVY-AAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQK 433
           SLY G+ +   +   P+VY   +AS+    S CL   L+   VAGKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 434 GLVVKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISA 493
           G VVK AGG GM+L NT T GEE +AD+H+LPA AVG+K G  IK Y  T+   TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 494 GTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHV 553
             TR+G++PSP+VAAFSSRGPN L+ +ILKPDL+APGVNILA W G + P+ L SD R V
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 554 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLP 613
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S   P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 614 STPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISK-KDFTCSGNKN 673
           S+P+D GAGH++P  A DPGLVYD   ++YF FLC  + S  Q+KV +K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 674 YKLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAP-STYKVSVTSKSPSVK 733
               +LNYP+ +            E T    +   RT+TN G   S+YKVSV S      
Sbjct: 672 KNPGNLNYPAISALFP--------ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGAS 731

Query: 734 IMVEPESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTW 773
           + V+P++L+F   +++ SYTVTF         E F  L W    H V SP+  TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE-FGGLVWKSTTHKVRSPVIITW 776

BLAST of Carg11874 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 721.1 bits (1860), Expect = 9.6e-208
Identity = 398/778 (51.16%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 13  LLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSAQ- 72
           + F+     C F+ +  SS  L  ++YI+H+ R++ P  F  H  W+ S L+S+  S Q 
Sbjct: 9   IFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 68

Query: 73  --TLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLEKSVSF 132
              LY Y+  VHGFS RL+  +   + +   +++VIP+   ++HTT TP FLG  ++   
Sbjct: 69  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 128

Query: 133 FPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLIGA 192
           +  S    +VIVGVLDTG+ PE  SF D+GLGP+P +WKGECE+G +F +SSCNRKLIGA
Sbjct: 129 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 188

Query: 193 RYFSKGYEAAFGAIDE--SQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGM 252
           R F +GY        +  ++ES+SPRD +GHG+HT++TAAGS V  ASL+ +A GTA GM
Sbjct: 189 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 248

Query: 253 AAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLG--GSSPDYYRDNVAIGAF 312
           A++AR+A YK+CW GGC+ SDILAAMD+A+ DGV+V+SLS+G  GS+P+Y+ D++AIGAF
Sbjct: 249 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 308

Query: 313 SATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLY 372
            AT  G+ VSCSAGN GP+  + +N+APWI TVGA T+DR+F      G+GK FTG SLY
Sbjct: 309 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 368

Query: 373 NGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVKE 432
            G+ L DS + +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG  VK 
Sbjct: 369 AGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 428

Query: 433 AGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTTRLG 492
           AGGAGMILANT   GEE  AD+HL+PA  VG K GD I+ YI T+ +PTA IS   T +G
Sbjct: 429 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 488

Query: 493 VQ-PSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFNII 552
              PSP VAAFSSRGPN LTP ILKPD+IAPGVNILAGW G VGPT LD D R V FNII
Sbjct: 489 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 548

Query: 553 SGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTPFD 612
           SGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE I+D+++G  S  F 
Sbjct: 549 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 608

Query: 613 IGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDF---TCSGNKNYKL 672
            GAGHV+P  AL+PGLVYD  +++Y AFLCA+ Y    I V  +       C  +K    
Sbjct: 609 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 668

Query: 673 EDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGA--PSTYKVSVTSKSPSVKIM 732
            DLNYPSF+V   +      GE      VKY R + N G+   + Y+V V S + +V+I 
Sbjct: 669 GDLNYPSFSVVFAST-----GE-----VVKYKRVVKNVGSNVDAVYEVGVKSPA-NVEID 728

Query: 733 VEPESLSFAKPNEQKSYTVTFVASAMPSG-----SESFARLEWSDGKHNVGSPIAFTW 773
           V P  L+F+K      Y VTF +  +  G        F  +EW+DG+H V SP+A  W
Sbjct: 729 VSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Carg11874 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-200
Identity = 382/779 (49.04%), Postives = 512/779 (65.73%), Query Frame = 0

Query: 11  WFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSSLKSVSDSA 70
           ++  FL++  S S +A+  +S      TYI+H+D    P  F  HF WY SSL S++ S 
Sbjct: 5   FYFFFLLTLSSPSSSASSSNS-----LTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 64

Query: 71  QTLYY-YNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLGLEKS--V 130
            ++ + Y+TV HGFS RLT ++A  +     +++VIPE    LHTTR+PEFLGL  +   
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 131 SFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSCNRKLI 190
                S    ++++GV+DTGV PE  SFDD GLGPVP+ WKG+C   ++F  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 191 GARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAGTAKGM 250
           GAR+F  GYEA  G ++E+ E +SPRD DGHG+HT++ +AG  V  AS  G+A G A GM
Sbjct: 185 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 244

Query: 251 AAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAIGAFSA 310
           A +AR+A YKVCW  GC+ SDILAA D A+ DGV+V+SLS+GG    YY D +AIGAF A
Sbjct: 245 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 304

Query: 311 TAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQSLYNG 370
             +G+FVS SAGNGGP + +++NVAPW+TTVGAGT+DRDFP  V LGNGK  +G S+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 KPLSDS-LIPIVYAAH--ASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLVVK 430
             L    + P+VY       +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 EAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYIS------TNANPTATIS 490
           + GG GMI+AN    GE  +AD H+LPA +VG   GD I+ YIS      ++ +PTATI 
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 AGTTRLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRH 550
              TRLG++P+P+VA+FS+RGPN  TP+ILKPD+IAPG+NILA W   +GP+G+ SD R 
Sbjct: 485 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 544

Query: 551 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGL 610
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S+G 
Sbjct: 545 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 604

Query: 611 PSTPFDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKN 670
            S+  D G+GHV+PT A+DPGLVYD T  DY  FLC  NY+   I  I+++   C G + 
Sbjct: 605 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 664

Query: 671 Y-KLEDLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKG-APSTYKVSVTSKSPSV 730
              + +LNYPSF+V  +         G +  +  + RT+TN G + S Y++ +       
Sbjct: 665 AGHVGNLNYPSFSVVFQQ-------YGESKMSTHFIRTVTNVGDSDSVYEIKIRPPR-GT 724

Query: 731 KIMVEPESLSFAKPNEQKSYTV----TFVASAMPSGSESFARLEWSDGKHNVGSPIAFT 772
            + VEPE LSF +  ++ S+ V    T V  +  + +     + WSDGK NV SP+  T
Sbjct: 725 TVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029314.10.0e+00100.00Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6598344.10.0e+0099.87Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961887.10.0e+0099.22subtilisin-like protease SBT1.7 [Cucurbita moschata][more]
XP_023545694.10.0e+0098.45subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo][more]
XP_022996498.10.0e+0097.81subtilisin-like protease SBT1.7 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O653511.8e-28063.79Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF62.4e-21955.66Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9FLI41.2e-21552.00Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Q9LVJ11.4e-20651.16Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
Q9LUM32.1e-19949.04Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1HBB40.0e+0099.22subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE... [more]
A0A6J1KAX60.0e+0097.81subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111491728 PE=3... [more]
A0A0A0LKN00.0e+0087.81Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1[more]
A0A6J1BPX80.0e+0087.71subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 P... [more]
A0A1S3BA540.0e+0086.90subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G67360.11.3e-28163.79Subtilase family protein [more]
AT2G05920.11.7e-22055.66Subtilase family protein [more]
AT5G51750.18.7e-21752.00subtilase 1.3 [more]
AT3G14067.19.6e-20851.16Subtilase family protein [more]
AT3G14240.11.5e-20049.04Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 545..561
score: 56.45
coord: 214..227
score: 38.27
coord: 136..155
score: 29.19
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 139..629
e-value: 1.0E-183
score: 613.3
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 112..620
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 139..584
e-value: 8.0E-47
score: 160.5
IPR003137PA domainPFAMPF02225PAcoord: 390..463
e-value: 1.2E-11
score: 44.5
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 38..113
e-value: 1.2E-12
score: 48.3
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 663..769
e-value: 1.5E-23
score: 82.9
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 20..113
e-value: 5.5E-17
score: 64.0
NoneNo IPR availableGENE3D3.50.30.30coord: 344..484
e-value: 1.0E-183
score: 613.3
NoneNo IPR availableGENE3D2.60.40.2310coord: 633..772
e-value: 1.1E-35
score: 124.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..226
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 26..771
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 109..616
score: 26.410862
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..20
score: 6.0
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 350..477
e-value: 1.61394E-46
score: 159.887
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 397..467
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 26..771
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 546..556
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 110..582
e-value: 3.50036E-140
score: 413.532

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11874-RACarg11874-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity