Carg11803 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11803
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA 4-demethylwyosine synthase (AdoMet-dependent)
Location	Carg_Chr05: 1039744 .. 1045632 (-)
RNA-Seq Expression	Carg11803
Synteny	Carg11803

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGTAATTTCTTCGGCTGTTTATCTGCATTTTATTTCTCACGATGAGATGATGAAGAAAGCTTTCGGCAGACATTACCTTTAATCATAGATTCCAAATTTGTGTTTTCCGGTGCACCCCCAAATTGTCGGAATTACTGCTTGTTTTCTAGGCAATTTCTGGGTGCCTTGTGAATCAATACAAATCTGGAATGACTTGATAGTTTATCTTTTGCAACGGTCTTAGTTGAAGCTTTACTTCTTTTCCATTTGTTGTCTGATTGGCTCTTGAAATGGTTTTTCTTATGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGGTAATGTCCCATACATTCTATTTTGCTTGACCACAGCGAGTCTTCTTTCCTTTTCACGGTCATTATAATATTTGATGTTTTGATACATTTAGTGGCAATGACATAGAACTCTCCGTTTCATAGATTAGAATTAAGATGTATGCCTCTTTACTCTGGTAAATGAATGATTTCTTTTCTTAGGAGGATGGATGATAGAATATGATTGAAGACTGCTAGGTGAATGGAAAAATAAGAATTTGGTGGCAGTGGCCTTCAAATCACTGAAAGTTACTAGAGTGTTTTTGGTGGCATCAGAGAAACTTTTGTTTGATATTCTTTGCTCGGCCAAATTTTACATCCAGCTGCTACTGATTTTATTTTTGAAATCCTTACTGTTTTGCCTACGTGACTGATATCTTCAATTTCCATAACATAAGCAGAAAGTTTCATGCTTTCTCATATTTTCTCAGTACATCCTTTTTATGTTGTTATGCTGATGCAAAATGTATATTGAGAGATAATTTTCTTTGATTTATCAACTTGCATTATGAATCCTTCTTCATCTGGGAACTTGAATTTAAAGTTGACTCCTCACTTTCCTTCCACACAACAAGGCTTTTCCTAATCTCTTAATTTTTTTTATAGACTGGTAGAAACTATCTATTGATTAGTGCTGTTGCTTGGATTAACTGTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCGAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCAGCAATCGATTTACACAAAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTTAGTGCTCCTGTTCTACTGCACTGGTGTGAGTAATATTTTCATGAAGATTGACCTTTCTTGGATAGACTTAATTGCTCCTGTGATTTGATGGTTGTAGAATCTGATTTTTTTTTTTTTGTTTGTCAATCATCAATATTGTTAATGTTTGTTATTTCTTTTAAAACTGTCAAAGAAATGCGCTGGCATTGCATGAGTTTTATGGCTAAATAGATATCAAGAGTAGTAGACCGCAAAGTAACGGCATTTTATACAGTTGAATCCCCCTTTGGGAGTTATAGAGATCTTTTAAGGAAGAAGTTCAAGTAACATATTAATCTAAGATTCCTGAAAATTTGGCTGCTTGAGAATTTAAAGTTGCAGTATTTTATTTTATAGTACCTATCATTGAGAATCCTGAGAGAATGCTTGAAATGAAAGTTATTTTTAAAATTTCCTTTTATTATTATTGTTATTATTTGTTTTTTTTTTTTGCAGTAATCCATGAAATTTTATCGATGAGTTTATGTTCACGTAACCTTTTTTTTTTTTTTTTTTTTTAATTGTTGGAAAAAGGTCATGTCAACGAATTTGAAAGACAATGACCAACGCTTGCTCCTTTACACAAACAAGTATAAAGCTAATTTTAGTAATCAGATACACTCATAACGTAAAATAAAAGTTCATGTACAATGCTAAAAAGTAAAAGCAAATTTTCTTTCGTTGGTAGTTGGGATTTTAGTGATGATGTGATTACAGAGTCATTAATGCATATCAATCTTGTTGATGAGTGATGTCTTCCTGCAGGTGTTACGCAGGAGAAATTGGAAGAAGGCTTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCACCGAAGGCGGATTTCAACTTTCTTAGTTACAAATGCTCAGTTTCCTGATAAAATAAAATCGTTGAAGCCGGTCACCCAGGTTTGCATGCTTTACAAATTTTCTTTTCTAAATAACATCAAATGTCAGGCTTATGCTCATTGATAACTAGATTACCTAGCTGATTTCAATGATGCATGCAGTTGTATGTAAGTGTAGATGCTGCAACGAAGGATAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGACTTTTGGGAGCGATTCATTGTAAGTTTCCTCCTACCCTCACCTGTTTCCAGGCCTGTCTCCTGAGCTAGACTGCATTGGAATTTATGAGAAAAGTAAATTTTATGGGGATGTTAAAATAAACAATGTTAACTTAAACTGTTCCCACCTCAAAACCTCACTTAGGCAATCGCCAATCTCTTCTTGCCCACACTGGGCGCTCAACAGTCAAATACATTTTTCTACAATCTCAGTTTGCCTGCCAAAGTAAAATTAAACAACAAATGTGCCTGACTCACCTAAAAGGGAGAAAAACCCTTCTAAAAGGCATTCCTACCTTGAAGGAAGCATTTCATGATGAACAGAGCCGATATTTTAGTAATTTTCATTAATTGGTGCTCGCTATCAGATATGCTCTCATGGAAACGCTCTGCCATCTAGTATCCATCCCCAATGAACTTATCTTGTGTATTTAAAAACCATAGTCTCCTATTAACTTTCTTCCTATGGTTATTTTATCAGCAATTAAAGTGATGGATTGTGAGAGAATTTCATTTCATTTGATATTTACTCAATTACTTTGGATGATTTGCAACTTGTGAGGTGTTTGTTGAATTCGATTGCTTTTAATTTGATTGCTTATATAAAATATATATATATATATATATATATATAATATAGTATATATTATATTATATATATAAATCGTTGATAGAAAATCAACTTGTGTGAAAGAACAACAAACAAGGAAAAGACACATGAAAAGAAAAAAGAGCTCGATCAAAAGAGGGGGGAGGGGAAACAATACTCAGAAAAACGTATAAGAAAGAACACCATCCTTCCAGTAACCTAAAAAAGAAAGAATTATGAAATAATTTTGAATTAAGAGCCTAAAGAGAAGCCTAAACCTAAAACAACAAATATTTACAAAATAAATTTCAATTAAGAGCCTAAAGAGAAGAACCACTAGATTCCACACCTATCCAAGAGCTATCTCTTTCTAGGAATACTCTTTTATTCCTTTCCAACTAGATAAACTGCAAAACAACAAAAAGAGCCAGCCGTTCTGCCAAAGAACCCGACCTTGTTACAAGTTGATTACATTGTTAGTATTTGTACGTAGTATTAGTTTAAAAGGCACATCAGAGGCTAAGTGTTGTTATGCAACTTATTAAGAATTATTTTATCAATAAAATTTTCTCTTAAGTCATAATGTTGAATATTAATAAATATTTAGAAAAGCTACAAAGGAGGCAATGCACAGGACTGCATCACGTCATTGTTGAATCTTTTTTGTTGATGAAATGTTGTTCAGCTTTGTATGTTGTTGCTTTGTTGTAGTTATCTACTAAGTAGTTTGCTATTCTTCTTTGTAAAAGACTGTCAGTTTCGTTCTTATCTATTATAAGAATCCCGACAGACATTCTGCTATCTAGTGTACATTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCTAGATGAGACTTGCATGATTTGTACTTTGGGGAAATGAGACTTTGATTGATTTCAACTTGTTATCCCATCAGAACTTAAGTTCTTTGATTAGTATGTATACTTAATCATGGTGCAAAAAAATTTGTGGACAAAACGAAATATTCTTTGAATTACTGATTTATTACTTTCCATAATATCGCAGGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACATTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCGAATCTTTTTAGTCTTGGTAAACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCGTGAGTTTCTGTTCTATTTATTTTGCATTAGGTGGGGAGGATATTGATTTGAAAATTGTGGGAGCATAACGAGTCTAATGTTCTTCTGTAGATCTAATACATCAAAGTTGACAATGGAGAACGTTCCTTGGCATTCTGATGTAAAGTCTTTTTCTGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCATTCATGTTGCGTTCTCTTGGCCAAAACTGACAAATTTAGAGTCGATGGGCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTTTGTTTGATGACAACTAATTTGCCATGAAACCTATGATGTTCAACCCCCTTCCTTTAACTTTTATCCTTCTCTAAAATTTTCAGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCGTGGGCTGTATATGGTGCTGACGAAGGTGGATTTGATCCTGTTCAATCTCGATACCGGAAGGAGCGACATCATAGACCAAAACCCACATCCTGATAGTTTTGAATGTCCGGGTCAGTTTTGTTTAACGGTCTTATCGATTTTCTTTGAGGACTTTTGTAGTTTATATTATTCTCTTCTGTGAAACAGCCTAGGTTGGCATTACTTATTTTTAGAAAGCGCCTTGCGTGGCATTTATGCCCGTATACATGTTTAAACTCGTTCTACTTTTCTAATTATAGAAGATATAGAGCTTGCTTGGTAACGAAATTTCTAGGTTAAAAGATAAACTTTAGCACGTAACAATTTAGTTACATTTTATTTTAACAATTGTAACAGTTTAATCCATATCATGAAAATTTTCATC

mRNA sequence

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGGTAATGTCCCATACATTCTATTTTGCTTGACCACAGCGAGTCTTCTTTCCTTTTCACGGTCATTATAATATTTGATGTTTTGATACATTTAGTGGCAATGACATAGAACTCTCCGTTTCATAGATTAGAATTAAGATGTATGCCTCTTTACTCTGGTAAATGAATGATTTCTTTTCTTAGGAGGATGGATGATAGAATATGATTGAAGACTGCTAGGTGAATGGAAAAATAAGAATTTGGTGGCAGTGGCCTTCAAATCACTGAAAGTTACTAGAGTGTTTTTGGTGGCATCAGAGAAACTTTTGTTTGATATTCTTTGCTCGGCCAAATTTTACATCCAGCTGCTACTGATTTTATTTTTGAAATCCTTACTGTTTTGCCTACGTGACTGATATCTTCAATTTCCATAACATAAGCAGAAAGTTTCATGCTTTCTCATATTTTCTCAGTACATCCTTTTTATGTTGTTATGCTGATGCAAAATGTATATTGAGAGATAATTTTCTTTGATTTATCAACTTGCATTATGAATCCTTCTTCATCTGGGAACTTGAATTTAAAGTTGACTCCTCACTTTCCTTCCACACAACAAGGCTTTTCCTAATCTCTTAATTTTTTTTATAGACTGGTAGAAACTATCTATTGATTAGTGCTGTTGCTTGGATTAACTGTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCGAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCAGCAATCGATTTACACAAAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGAAGAAGGCTTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCACCGAAGGCGGATTTCAACTTTCTTAGTTACAAATGCTCAGTTTCCTGATAAAATAAAATCGTTGAAGCCGGTCACCCAGTTGTATGTAAGTGTAGATGCTGCAACGAAGGATAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGACTTTTGGGAGCGATTCATTGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACATTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCGAATCTTTTTAGTCTTGGTAAACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCATCTAATACATCAAAGTTGACAATGGAGAACGTTCCTTGGCATTCTGATGTAAAGTCTTTTTCTGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCATTCATGTTGCGTTCTCTTGGCCAAAACTGACAAATTTAGAGTCGATGGGCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCGTGGGCTGTATATGGTGCTGACGAAGGTGGATTTGATCCTGTTCAATCTCGATACCGGAAGGAGCGACATCATAGACCAAAACCCACATCCTGATAGTTTTGAATGTCCGGGTCAGTTTTGTTTAACGGTCTTATCGATTTTCTTTGAGGACTTTTGTAGTTTATATTATTCTCTTCTGTGAAACAGCCTAGGTTGGCATTACTTATTTTTAGAAAGCGCCTTGCGTGGCATTTATGCCCGTATACATGTTTAAACTCGTTCTACTTTTCTAATTATAGAAGATATAGAGCTTGCTTGGTAACGAAATTTCTAGGTTAAAAGATAAACTTTAGCACGTAACAATTTAGTTACATTTTATTTTAACAATTGTAACAGTTTAATCCATATCATGAAAATTTTCATC

Coding sequence (CDS)

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCGCCCTTCTGGCTCTTCTCTCTGCCTCCACTTTGTATTGCTTCTACAAGTCCCGCCGTCTCAGAAAACTTAAACTCTCCTTAAACCCTACGGGTTGTTCTCGCAGACCCAAACTTTTCTTCATTTCCCAAACTGGTACCTCAAAGGCCCTAGCGCATCGTCTGCTTAACGTTTTGAATGCAAATGAGTTGGCGTTTGATATTGTCGATCCCAAGGATTTTGAGCCTGAAGATCTTTTCAAGGAGACTTTAGTTTTGTTCGTTGCTTCCACCTGGGAAGATGGAGGACCGCCTCCTCATGCTAAATTCCTCGCCAGTTGGCTTTCTGAGAGCGCGGAGGATTTCAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAATTTGCTGTATTTGGCGTTGGTAGTCGGGCTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGATGAAGGCATTGGGTGCGAAGGAGATATTAGCGGTTGGTGAGGGAGACGTTGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTCGGCAATTGCTGAAGGTTTTGAAGGTTGATGCGGTGGGCACTGGTGCGGATTTATATTCTGGGATTGATGAGGACTTTGATGCTGAAAGTGTTGAGGAGGATGACGAAGATGCCGACTCTGGGGAGGAGGACATCGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACCAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAAGTTATGGTTACCCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGGTATAAAATTATTGGCTCACACAGCGGTGTGAAAATATGTAGATGGACAAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAGCATTCGTTTTATGGCATAGAAAGTCATCGGTAA

Protein sequence

MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHR

Homology

[Close]

BLAST of Carg11803 vs. NCBI nr
Match: KAG7029383.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. NCBI nr
Match: KAG6598440.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 622.9 bits (1605), Expect = 1.6e-174
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. NCBI nr
Match: XP_023546392.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621.7 bits (1602), Expect = 3.5e-174
Identity = 316/317 (99.68%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. NCBI nr
Match: XP_022962153.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita moschata])

HSP 1 Score: 617.5 bits (1591), Expect = 6.6e-173
Identity = 314/317 (99.05%), Postives = 315/317 (99.37%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 313/317 (98.74%), Postives = 315/317 (99.37%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKE LVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. ExPASy Swiss-Prot
Match: Q8RXN5 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 7.5e-103
Identity = 204/323 (63.16%), Postives = 245/323 (75.85%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLK-LSLNPTGC--SRRPKLFFISQTGTSKAL 63
           SS   +LA +ALLSA+T YC +K RRL+ LK LSLNP+    + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 123
           A RL  +  +N++AFDIVDP  +EPEDL KETLVLF+ASTW+ G PP + +FL +WL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRQLLKVLKVDAV--GTGADLYSGIDED----FDAESVEEDDEDADSGEED 243
           ++D+ F DW   +++VLK  +     G     G  ED    +D+   E++D DAD G   
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGG--- 244

Query: 244 IVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRW 303
           IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRW
Sbjct: 245 IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRW 304

Query: 304 TKSQLRGRGGCYKHSFYGIESHR 318
           TKSQLRGRGGCYKHSFYGIESHR
Sbjct: 305 TKSQLRGRGGCYKHSFYGIESHR 322

[Close]

BLAST of Carg11803 vs. ExPASy Swiss-Prot
Match: Q8H8N3 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-83
Identity = 178/322 (55.28%), Postives = 221/322 (68.63%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNP--TGCSRRPKLFFISQTGTSKALA 63
           +S ++ LALL LLS+S+++  YKS RLR+      P   G +  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSESA 123
             L   L    +     D   F+P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGGD 183
            DFR G+ LLS  +FAVFGVGSRAYGETFNA AR FS+ ++ALGA E++AVGEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEED--IVDLE 243
           ++  F++W  ++++V+K + +G G   ++G  + FD    EE D+D D  E D   VD+E
Sbjct: 192 LEVVFEEWCGRVVRVVKGEEIGEG---HNGESDGFDELEEEESDDDDDEEEVDGGEVDME 251

Query: 244 DIAGKGP-SRKSTNVVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWT 303
           DIAGK P +R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKICRWT
Sbjct: 252 DIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWT 311

Query: 304 KSQLRGRGGCYKHSFYGIESHR 318
           KSQLRGRGGCYKHSFYGIESHR
Sbjct: 312 KSQLRGRGGCYKHSFYGIESHR 330

[Close]

BLAST of Carg11803 vs. ExPASy Swiss-Prot
Match: Q2KHP8 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 118/328 (35.98%), Postives = 177/328 (53.96%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +    L  ++++ K+++P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG PP  A++   WL E++ DFR G   L   ++AVFG+G+  Y   +N V ++  K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRQLLKVLKVDAVG------------- 226
             L A  ++   EGD +      G I+  F+ W R+ L  LK    G             
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGK 263

Query: 227 ------TGADLYSGIDEDFDAESVEEDDED------ADSGEED-------IVDLEDIAGK 286
                 +  +     +E+ +  S  ED ED      +DS  E+       ++D+ED+ GK
Sbjct: 264 CKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-GK 323

Query: 287 GPS--RKSTNVVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSGV 318
             S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSGV
Sbjct: 324 AMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGV 383

[Close]

BLAST of Carg11803 vs. ExPASy Swiss-Prot
Match: Q08C92 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-44
Identity = 115/325 (35.38%), Postives = 166/325 (51.08%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPEDLFKE-----TLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + A  +  +++D KDF+PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+  Y   FN V++   K +
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILAVGEGDVD-----GGDIDKCFDDWSRQLLKVLKVDAVG------------- 226
             L A  I+  GEGD +      G +   F  W  + L  L+  A G             
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKAS 259

Query: 227 ---------TGADLYSGIDEDFDAESVEEDDEDADSGEED------IVDLEDIAG----- 286
                       D +S  +++   E + E   D +S  ED      ++D+ED+       
Sbjct: 260 CKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHM 319

Query: 287 -KGPSR-----KSTNVVETNGKLNG-----KKVMVTPVIRASLEKQGYKIIGSHSGVKIC 318
            K   R     + +  V+ NG+        ++ M+TP +R SL KQGYK+IGSHSGVK+ 
Sbjct: 320 KKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLR 379

[Close]

BLAST of Carg11803 vs. ExPASy Swiss-Prot
Match: Q8BJM7 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Mus musculus OX=10090 GN=Tyw1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-43
Identity = 114/324 (35.19%), Postives = 167/324 (51.54%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNANELAFDIVDPKDFEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + +L   I++ K+++P+D     +  +T+  F+ +T+
Sbjct: 72  KIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATY 131

Query: 107 EDGGPPPHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRM 166
            DG P   A++   WL ESA DFR G   L   ++AVFG+G  AY   FN V+ +  K +
Sbjct: 132 TDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWL 191

Query: 167 KALGAKEILAVGEGDVDG-----GDIDKCFDDWSRQLLKVLKV----DAVGTGADLYSGI 226
             LGA+ +L  GEGD +      G I+  F  W  + +  L+     +    G +   G 
Sbjct: 192 WMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGK 251

Query: 227 DEDFDAESVE--------------EDDEDADSGEED------------IVDLEDIAG--K 286
            E       E              E++E  +S  ED            +VD+ED+     
Sbjct: 252 CESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMN 311

Query: 287 GPSRKSTNVVETNGKL----------NGK-KVMVTPVIRASLEKQGYKIIGSHSGVKICR 318
              R+       +GK           +G+ + M+TP +R +L KQGY++IGSHSGVK+CR
Sbjct: 312 PVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCR 371

[Close]

BLAST of Carg11803 vs. ExPASy TrEMBL
Match: A0A6J1HDZ5 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662 GN=LOC111462691 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.2e-173
Identity = 314/317 (99.05%), Postives = 315/317 (99.37%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. ExPASy TrEMBL
Match: A0A6J1K9S7 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 GN=LOC111492394 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.4e-173
Identity = 313/317 (98.74%), Postives = 315/317 (99.37%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+NELAFDIVDPKDFEPEDLFKE LVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADSGEEDIVDLED 240
           DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDAD GEEDIVDLED
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDEDADPGEEDIVDLED 240

Query: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300
           IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR
Sbjct: 241 IAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLR 300

Query: 301 GRGGCYKHSFYGIESHR 318
           GRGGCYKHSFYGIESHR
Sbjct: 301 GRGGCYKHSFYGIESHR 317

[Close]

BLAST of Carg11803 vs. ExPASy TrEMBL
Match: A0A5A7VC83 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005790 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 286/319 (89.66%), Postives = 300/319 (94.04%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMKALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADSGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE  ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHR 318
           LRGRGGCYKHSFYGIESHR
Sbjct: 301 LRGRGGCYKHSFYGIESHR 319

[Close]

BLAST of Carg11803 vs. ExPASy TrEMBL
Match: A0A1S3BAY2 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LOC103487965 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.3e-155
Identity = 285/319 (89.34%), Postives = 300/319 (94.04%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGET+NAVARDFSKRMK+LGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEE--DDEDADSGEEDIVDL 240
           DIDKCFDDWSR+LLKV+KVDA G G +L SGI  D DA SVEE  ++ED D GEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHR 318
           LRGRGGCYKHSFYGIESHR
Sbjct: 301 LRGRGGCYKHSFYGIESHR 319

[Close]

BLAST of Carg11803 vs. ExPASy TrEMBL
Match: A0A0A0LPA4 (tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 8.7e-155
Identity = 284/318 (89.31%), Postives = 298/318 (93.71%), Query Frame = 0


Query: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60
           MS SSRSAKL +LALLSASTLYCFYKSRRLRKLKLSLNPT  SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120
           AHRLLNVLN+N L FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180
           AEDFRVGSLLLSQCKF+VFGVGSR YGETFNAVARDFSKR+KALGAKEIL +GEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEED-DEDADSGEEDIVDLE 240
           DIDKCFDDWSR+LLKV+K DA G G +L SGI  D DA SVEED +ED D GEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHR 318
           RGRGGCYKHSFYGIESHR
Sbjct: 301 RGRGGCYKHSFYGIESHR 318

[Close]

BLAST of Carg11803 vs. TAIR 10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein )

HSP 1 Score: 375.2 bits (962), Expect = 5.3e-104
Identity = 204/323 (63.16%), Postives = 245/323 (75.85%), Query Frame = 0


Query: 4   SSRSAKLALLALLSASTLYCFYKSRRLRKLK-LSLNPTGC--SRRPKLFFISQTGTSKAL 63
           SS   +LA +ALLSA+T YC +K RRL+ LK LSLNP+    + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNANELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 123
           A RL  +  +N++AFDIVDP  +EPEDL KETLVLF+ASTW+ G PP + +FL +WL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 183
           AEDFRVGSLLLS CKFAVFGVGSRAYGE++NAVA++ S RM  LG  E++ VGEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRQLLKVLKVDAV--GTGADLYSGIDED----FDAESVEEDDEDADSGEED 243
           ++D+ F DW   +++VLK  +     G     G  ED    +D+   E++D DAD G   
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGG--- 244

Query: 244 IVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRW 303
           IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRW
Sbjct: 245 IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRW 304

Query: 304 TKSQLRGRGGCYKHSFYGIESHR 318
           TKSQLRGRGGCYKHSFYGIESHR
Sbjct: 305 TKSQLRGRGGCYKHSFYGIESHR 322

The following BLAST results are available for this feature:

BLAST of Carg11803 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029383.1	1.6e-174	100.00	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucu...	[more]
KAG6598440.1	1.6e-174	100.00	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, partial [Cucu...	[more]
XP_023546392.1	3.5e-174	99.68	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita pep...	[more]
XP_022962153.1	6.6e-173	99.05	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita mos...	[more]
XP_022997485.1	1.1e-172	98.74	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita max...	[more]

BLAST of Carg11803 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXN5	7.5e-103	63.16	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
Q8H8N3	3.9e-83	55.28	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
Q2KHP8	1.1e-45	35.98	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopu...	[more]
Q08C92	1.6e-44	35.38	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio ...	[more]
Q8BJM7	3.0e-43	35.19	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Mus mu...	[more]

BLAST of Carg11803 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HDZ5	3.2e-173	99.05	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita moschata OX=3662...	[more]
A0A6J1K9S7	5.4e-173	98.74	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucurbita maxima OX=3661 G...	[more]
A0A5A7VC83	1.0e-155	89.66	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BAY2	2.3e-155	89.34	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LPA4	8.7e-155	89.31	tRNA 4-demethylwyosine synthase (AdoMet-dependent) OS=Cucumis sativus OX=3659 GN...	[more]

BLAST of Carg11803 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75200.1	5.3e-104	63.16	flavodoxin family protein / radical SAM domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 95..106 score: 39.97 coord: 47..60 score: 35.6 coord: 133..143 score: 47.16 coord: 158..177 score: 33.36
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 250..317 e-value: 1.1E-24 score: 89.0
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 e-value: 9.6E-26 score: 90.7
IPR008254	Flavodoxin/nitric oxide synthase	PROSITE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 22.03581
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 26..205 e-value: 7.9E-40 score: 138.2
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 47..198
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..317
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 3..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11803-RA	Carg11803-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0031591	wybutosine biosynthetic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0102521	tRNA-4-demethylwyosine synthase activity
molecular_function	GO:0003824	catalytic activity