Carg11771 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11771
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	V-type proton ATPase subunit a
Location	Carg_Chr05: 1216470 .. 1217347 (-)
RNA-Seq Expression	Carg11771
Synteny	Carg11771

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGTATCAACCAGTGTAAATCAACTCATTGTGGGCTATATTCCTCCGTGCGGGTGACTTATACCCTCCGTTTTTCAGAGATACTAATACCATTGGTTTAATAAAGGTTCGTGACGCGTATCCATTTGGTGTGGATCCGAAGTGGCAGGGCACTCGAAGTGAGTTACCTTTTCTCAAATCTTTGAAGATGACGATGTCTATCCTACTGGAGTAGCTCAAATGAACAGAGGAATTATATTGAGCTACTTCAAAGCAAAATTCTTTGGAGAAAACATTAACATCTGGTTAGTTGCTTTAAGATGGATCCATTGTATCATCATTAAAATCATTTCTTTATTGAATTGTTAATTAATTGGCAGTCTTTCCGTAGAATTAATTGTTTGAAAATTTTCCTCCGGATGGCGGATTCTTGAATGAGATGTAATGTAGTCAATTATATGCAGTTGATGAATAAACTTTTAAAAAAAATATTGTTTTTATAAATTATTCAATTAGGCCTCTTCAAACCACATCCTAAAATTCTAGTGAAGATTCTGTTTTCTTTATTCTAACAAGTGTAAATTTTGTTTCATTGTTTCAGGTACCAATTTGTTCTACAGATGATTTTCATGAACAGTTTATGCGGTTACCTCTCACTTCTCATAATTGTAACGTGGTGCTCTGGATCTCAAGCTCTGTATCACGTAATGATCTATATAGGCGAAAACCAGCTTTTTCCAGGACAGTTCCTTCAGGTTCTTCATGCTTTGATGTGCTTCAAATTTCTTTTCATAATACTTGATTAGAATCAACTATTAATAACTATGTTTTTACACTTCCAGCATCCGTTAATTCTGTCTGCCCTCACTACCTTCTCTGTTGAAGAAACAACATGA

mRNA sequence

ATGCAGGTATCAACCAGTGTAAATCAACTCATTGTGGGCTATATTCCTCCGTGCGGAGATACTAATACCATTGGTTTAATAAAGGTTCGTGACGCGTATCCATTTGGTGTGGATCCGAAGTGGCAGGGCACTCGAAATGACGATGTCTATCCTACTGGAGTAGCTCAAATGAACAGAGGAATTATATTGAGCTACTTCAAAGCAAAATTCTTTGGAGAAAACATTAACATCTGTCTTTCCGTAGAATTAATTGTACCAATTTGTTCTACAGATGATTTTCATGAACAGTTTATGCGGTTACCTCTCACTTCTCATAATTGTAACGTGGTGCTCTGGATCTCAAGCTCTGTATCACGTAATGATCTATATAGGCGAAAACCAGCTTTTTCCAGGACAGTTCCTTCAGGTTCTTCATGCTTTGATAATCAACTATTAATAACTATGTTTTTACACTTCCAGCATCCGTTAATTCTGTCTGCCCTCACTACCTTCTCTGTTGAAGAAACAACATGA

Coding sequence (CDS)

ATGCAGGTATCAACCAGTGTAAATCAACTCATTGTGGGCTATATTCCTCCGTGCGGAGATACTAATACCATTGGTTTAATAAAGGTTCGTGACGCGTATCCATTTGGTGTGGATCCGAAGTGGCAGGGCACTCGAAATGACGATGTCTATCCTACTGGAGTAGCTCAAATGAACAGAGGAATTATATTGAGCTACTTCAAAGCAAAATTCTTTGGAGAAAACATTAACATCTGTCTTTCCGTAGAATTAATTGTACCAATTTGTTCTACAGATGATTTTCATGAACAGTTTATGCGGTTACCTCTCACTTCTCATAATTGTAACGTGGTGCTCTGGATCTCAAGCTCTGTATCACGTAATGATCTATATAGGCGAAAACCAGCTTTTTCCAGGACAGTTCCTTCAGGTTCTTCATGCTTTGATAATCAACTATTAATAACTATGTTTTTACACTTCCAGCATCCGTTAATTCTGTCTGCCCTCACTACCTTCTCTGTTGAAGAAACAACATGA

Protein sequence

MQVSTSVNQLIVGYIPPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPTGVAQMNRGIILSYFKAKFFGENINICLSVELIVPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRNDLYRRKPAFSRTVPSGSSCFDNQLLITMFLHFQHPLILSALTTFSVEETT

Homology

[Close]

BLAST of Carg11771 vs. NCBI nr
Match: KAG7029412.1 (V-type proton ATPase subunit a1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 355.5 bits (911), Expect = 2.5e-94
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MQVSTSVNQLIVGYIPPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPTGVAQMNRG 60
           MQVSTSVNQLIVGYIPPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPTGVAQMNRG
Sbjct: 1   MQVSTSVNQLIVGYIPPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPTGVAQMNRG 60

Query: 61  IILSYFKAKFFGENINICLSVELIVPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRN 120
           IILSYFKAKFFGENINICLSVELIVPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRN
Sbjct: 61  IILSYFKAKFFGENINICLSVELIVPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRN 120

Query: 121 DLYRRKPAFSRTVPSGSSCFDNQLLITMFLHFQHPLILSALTTFSVEETT 171
           DLYRRKPAFSRTVPSGSSCFDNQLLITMFLHFQHPLILSALTTFSVEETT
Sbjct: 121 DLYRRKPAFSRTVPSGSSCFDNQLLITMFLHFQHPLILSALTTFSVEETT 170

[Close]

BLAST of Carg11771 vs. NCBI nr
Match: KAG6598477.1 (V-type proton ATPase subunit a3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-21
Identity = 52/53 (98.11%), Postives = 52/53 (98.11%), Query Frame = 0


Query: 85  VPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRNDLYRRKPAFSRTVPSGS 138
           VPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRNDLYRRKPAFSRTVPS S
Sbjct: 253 VPICSTDDFHEQFMRLPLTSHNCNVVLWISSSVSRNDLYRRKPAFSRTVPSAS 305

[Close]

BLAST of Carg11771 vs. NCBI nr
Match: KAE8057188.1 (hypothetical protein FH972_013897 [Carpinus fangiana])

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-15
Identity = 43/72 (59.72%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P C D +T+GLIKVRD YPFGVDPKW GTR++  Y            GVAQMN GIILSY
Sbjct: 495 PSCSDASTVGLIKVRDTYPFGVDPKWHGTRSELPYLNSLKMKMSILLGVAQMNLGIILSY 554

Query: 76  FKAKFFGENINI 78
           F A+FFG+++NI
Sbjct: 555 FNARFFGDDLNI 566

[Close]

BLAST of Carg11771 vs. NCBI nr
Match: TXG52143.1 (hypothetical protein EZV62_021312 [Acer yangbiense])

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-15
Identity = 45/72 (62.50%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P C D  TIGL+KVRD YPFGVDPKW GTR++  +            GVAQMN GIILSY
Sbjct: 504 PSCRDAFTIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSILLGVAQMNLGIILSY 563

Query: 76  FKAKFFGENINI 78
           F AKFFG NINI
Sbjct: 564 FNAKFFGNNINI 575

[Close]

BLAST of Carg11771 vs. NCBI nr
Match: XP_007158764.1 (hypothetical protein PHAVU_002G179900g [Phaseolus vulgaris] >ESW30758.1 hypothetical protein PHAVU_002G179900g [Phaseolus vulgaris])

HSP 1 Score: 91.7 bits (226), Expect = 6.7e-15
Identity = 43/72 (59.72%), Postives = 50/72 (69.44%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P CGD +TIGLIKVRD YPFGVDPKW GTR++  Y            GV+QMN GII+SY
Sbjct: 503 PSCGDASTIGLIKVRDTYPFGVDPKWHGTRSELPYLNSLKMKMSILLGVSQMNLGIIMSY 562

Query: 76  FKAKFFGENINI 78
           + AK+F  NINI
Sbjct: 563 YNAKYFENNINI 574

[Close]

BLAST of Carg11771 vs. ExPASy Swiss-Prot
Match: Q8RWZ7 (V-type proton ATPase subunit a1 OS=Arabidopsis thaliana OX=3702 GN=VHA-a1 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.7e-14
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C D  T+GLIK RD YPFGVDP W+G+R +  Y            G+AQMN G+ILS+F 
Sbjct: 508 CSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKMSILLGIAQMNLGLILSFFN 567

[Close]

BLAST of Carg11771 vs. ExPASy Swiss-Prot
Match: Q9SJT7 (V-type proton ATPase subunit a2 OS=Arabidopsis thaliana OX=3702 GN=VHA-a2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 37/70 (52.86%), Postives = 44/70 (62.86%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C +  TIGLIK RD YPFGVDP W GTR++  +            GVAQMN GII+S+F 
Sbjct: 510 CSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMSILIGVAQMNLGIIMSFFN 569

[Close]

BLAST of Carg11771 vs. ExPASy Swiss-Prot
Match: Q8W4S4 (V-type proton ATPase subunit a3 OS=Arabidopsis thaliana OX=3702 GN=VHA-a3 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-13
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C +  TIGLIKVRD YPFG+DP W G+R++  +            GV+QMN GII+SYF 
Sbjct: 509 CSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMSILLGVSQMNLGIIMSYFN 568

[Close]

BLAST of Carg11771 vs. ExPASy TrEMBL
Match: A0A5N6RBM1 (V-type proton ATPase subunit a OS=Carpinus fangiana OX=176857 GN=FH972_013897 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.1e-15
Identity = 43/72 (59.72%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P C D +T+GLIKVRD YPFGVDPKW GTR++  Y            GVAQMN GIILSY
Sbjct: 495 PSCSDASTVGLIKVRDTYPFGVDPKWHGTRSELPYLNSLKMKMSILLGVAQMNLGIILSY 554

Query: 76  FKAKFFGENINI 78
           F A+FFG+++NI
Sbjct: 555 FNARFFGDDLNI 566

[Close]

BLAST of Carg11771 vs. ExPASy TrEMBL
Match: A0A5C7H531 (V-type proton ATPase subunit a OS=Acer yangbiense OX=1000413 GN=EZV62_021312 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.9e-15
Identity = 45/72 (62.50%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P C D  TIGL+KVRD YPFGVDPKW GTR++  +            GVAQMN GIILSY
Sbjct: 504 PSCRDAFTIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSILLGVAQMNLGIILSY 563

Query: 76  FKAKFFGENINI 78
           F AKFFG NINI
Sbjct: 564 FNAKFFGNNINI 575

[Close]

BLAST of Carg11771 vs. ExPASy TrEMBL
Match: V7CN33 (V-type proton ATPase subunit a OS=Phaseolus vulgaris OX=3885 GN=PHAVU_002G179900g PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.3e-15
Identity = 43/72 (59.72%), Postives = 50/72 (69.44%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P CGD +TIGLIKVRD YPFGVDPKW GTR++  Y            GV+QMN GII+SY
Sbjct: 503 PSCGDASTIGLIKVRDTYPFGVDPKWHGTRSELPYLNSLKMKMSILLGVSQMNLGIIMSY 562

Query: 76  FKAKFFGENINI 78
           + AK+F  NINI
Sbjct: 563 YNAKYFENNINI 574

[Close]

BLAST of Carg11771 vs. ExPASy TrEMBL
Match: A0A2K3MJ11 (V-type proton ATPase subunit a (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g046898 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 5.6e-15
Identity = 43/72 (59.72%), Postives = 50/72 (69.44%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P C D+ TIGLIKVRD YPFGVDPKW GTR++  +            GV+QMN GIILSY
Sbjct: 163 PSCRDSTTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMSILLGVSQMNLGIILSY 222

Query: 76  FKAKFFGENINI 78
           + AK+FG NINI
Sbjct: 223 YNAKYFGNNINI 234

[Close]

BLAST of Carg11771 vs. ExPASy TrEMBL
Match: A0A164UZN6 (V-type proton ATPase subunit a OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_023032 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.3e-15
Identity = 43/72 (59.72%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 16  PPCGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSY 75
           P CGD+ T+GLIKVRD YPFGVDP W GTR++  Y            GVAQMN GIILS+
Sbjct: 436 PSCGDSTTMGLIKVRDTYPFGVDPAWHGTRSELPYLNSLKMKMSILLGVAQMNLGIILSF 495

Query: 76  FKAKFFGENINI 78
           F AKFFG  +NI
Sbjct: 496 FNAKFFGSAVNI 507

[Close]

BLAST of Carg11771 vs. TAIR 10
Match: AT2G28520.1 (vacuolar proton ATPase A1 )

HSP 1 Score: 78.6 bits (192), Expect = 5.5e-15
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C D  T+GLIK RD YPFGVDP W+G+R +  Y            G+AQMN G+ILS+F 
Sbjct: 508 CSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKMSILLGIAQMNLGLILSFFN 567

[Close]

BLAST of Carg11771 vs. TAIR 10
Match: AT2G21410.1 (vacuolar proton ATPase A2 )

HSP 1 Score: 77.8 bits (190), Expect = 9.4e-15
Identity = 37/70 (52.86%), Postives = 44/70 (62.86%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C +  TIGLIK RD YPFGVDP W GTR++  +            GVAQMN GII+S+F 
Sbjct: 510 CSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMSILIGVAQMNLGIIMSFFN 569

[Close]

BLAST of Carg11771 vs. TAIR 10
Match: AT4G39080.1 (vacuolar proton ATPase A3 )

HSP 1 Score: 77.0 bits (188), Expect = 1.6e-14
Identity = 35/70 (50.00%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 18  CGDTNTIGLIKVRDAYPFGVDPKWQGTRNDDVYPT----------GVAQMNRGIILSYFK 77
           C +  TIGLIKVRD YPFG+DP W G+R++  +            GV+QMN GII+SYF 
Sbjct: 509 CSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMSILLGVSQMNLGIIMSYFN 568

The following BLAST results are available for this feature:

BLAST of Carg11771 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029412.1	2.5e-94	100.00	V-type proton ATPase subunit a1, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6598477.1	1.7e-21	98.11	V-type proton ATPase subunit a3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAE8057188.1	2.3e-15	59.72	hypothetical protein FH972_013897 [Carpinus fangiana]	[more]
TXG52143.1	4.0e-15	62.50	hypothetical protein EZV62_021312 [Acer yangbiense]	[more]
XP_007158764.1	6.7e-15	59.72	hypothetical protein PHAVU_002G179900g [Phaseolus vulgaris] >ESW30758.1 hypothet...	[more]

BLAST of Carg11771 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWZ7	7.7e-14	50.00	V-type proton ATPase subunit a1 OS=Arabidopsis thaliana OX=3702 GN=VHA-a1 PE=2 S...	[more]
Q9SJT7	1.3e-13	52.86	V-type proton ATPase subunit a2 OS=Arabidopsis thaliana OX=3702 GN=VHA-a2 PE=1 S...	[more]
Q8W4S4	2.3e-13	50.00	V-type proton ATPase subunit a3 OS=Arabidopsis thaliana OX=3702 GN=VHA-a3 PE=1 S...	[more]

BLAST of Carg11771 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5N6RBM1	1.1e-15	59.72	V-type proton ATPase subunit a OS=Carpinus fangiana OX=176857 GN=FH972_013897 PE...	[more]
A0A5C7H531	1.9e-15	62.50	V-type proton ATPase subunit a OS=Acer yangbiense OX=1000413 GN=EZV62_021312 PE=...	[more]
V7CN33	3.3e-15	59.72	V-type proton ATPase subunit a OS=Phaseolus vulgaris OX=3885 GN=PHAVU_002G179900...	[more]
A0A2K3MJ11	5.6e-15	59.72	V-type proton ATPase subunit a (Fragment) OS=Trifolium pratense OX=57577 GN=L195...	[more]
A0A164UZN6	7.3e-15	59.72	V-type proton ATPase subunit a OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_...	[more]

BLAST of Carg11771 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28520.1	5.5e-15	50.00	vacuolar proton ATPase A1	[more]
AT2G21410.1	9.4e-15	52.86	vacuolar proton ATPase A2	[more]
AT4G39080.1	1.6e-14	50.00	vacuolar proton ATPase A3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002490	V-type ATPase, V0 complex, 116kDa subunit family	PANTHER	PTHR11629	VACUOLAR PROTON ATPASES	coord: 17..79
None	No IPR available	PANTHER	PTHR11629:SF101	V-TYPE PROTON ATPASE SUBUNIT A	coord: 17..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11771-RA	Carg11771-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902600	proton transmembrane transport
biological_process	GO:0007035	vacuolar acidification
cellular_component	GO:0033179	proton-transporting V-type ATPase, V0 domain
cellular_component	GO:0016471	vacuolar proton-transporting V-type ATPase complex
molecular_function	GO:0051117	ATPase binding
molecular_function	GO:0015078	proton transmembrane transporter activity