Carg11547 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11547
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGlutathione gamma-glutamylcysteinyltransferase
LocationCarg_Chr17: 9677140 .. 9680353 (-)
RNA-Seq ExpressionCarg11547
SyntenyCarg11547
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCTGCTTATTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCAGTCCAAATTCGATACTTGTTTTCCCTTATTAATCGAGAGCTCATTTTTCATTGGATCTGCTACTAGCATTACTAACAATCGATGAATTACAATCCCATACCAATTTTAGTCCTCAGATATACTGTAGTATCTGCTTCAGTATCTTGTATGCATATATCGACCAATGCATTGATTTTTCTTTTCAGTCATTCTAATACTATGCTGGAAGTCCCTTTTCCAGTTTGGAGAACATTCGCAATTTCAGTTCCAAACAGGATTATTTTGTGCCCTGGCTATTTTTTGCTAATTGGATAGGACAAAACTGTTCTCTGTCAATGAGCAATATTTAGACGCAAGAAAAGAGAGGAGATTTGGATGTACCTTCATAGCAACAGGACTCATGTAAGTTTGATGAAACGATTGTTCTTGTTTTCTTCAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTGATGATTTTCGCAAGTATGTCTTGTCATGTTCATCTTCTGAGGACTGCCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGGTATATCGAAGTATATATCTTCACCAATGGTCTTACTATATGTCCTCAAAATTAGCTGTAGTAACTGAAAGAGGTGTATAGTTTTGTAAATCTAAACCAATGAGCTGAAGTTTAATATATTATATATAAGAAAATACTAATATAATAGCACAAACTCACCACCGGCAGATATAGTTTTCTTTGAGCTTTCCCTTATGGACTTACCTTCGAGGTTTTAAAATGAGTCTACTTGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTTGTTCTCTCACAATGGTTCAAGATTGGTTCAAGATGGGCCCTCGTTCATGAGAAACAACTTTTGACGGTGTATTTGATCACTTGTCATATTTGATCACTTGTCATATTTGATCACTTGTCATAGGAGTTAATTATAGGCTTGTCTTTAACAAAATAATTGGTTTCAGACTGGAAGTGGCCATTTTTCCCCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCTCACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGGTAAACCCATATTCTCATTTCTCGTGTTTATTTGTGGAGTAGCTTCTGTTCTGCAGATCCATGTTCTGAAGTTTTCTTTTTAACGCAGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGGTACATCCTTTTCATAAACTTCAAAGTTGTTTGATAAGTCTCTAAACTTCAAAGTGTATGTTTTTAGGTCTTCTCGTCTACGTATGTGTTAAGAATTAATATTGAACACTGGTCGTAAATTTGTTTTGTGCAGAGTTGTCAGCATGACGGTTGGAATAACATTATAAAATACTTAACAGAAGAAATTCCCCAACTTTTGAAAACAGAAGAGGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGTTGTTCTCTAGAGCTTCCTTCTGTTATTTTTCTTGCTATGATTTGTTTTATGAGGTTTAAGTATGGGAATCCCAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTATGCAAAGGCTTGACATCTTCGTTTGACACAGATGAATTACATGCCATTGCTGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTGGGCTGATCGAAGTTGCTGCAAAACCGATATCCATCATCTGAAAGGTGAAAATGAGAAGGCAGCAGTGGTGGTGTCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTAATCCAGCTTTGAGCTTCTTCGTTTCCTTTCTGCTTTGGTTTGAAAAAAAAAAAAAAAAAGTATATCCATATTATTCCTTGCAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAATCTTCCTCATCTTCTTCATATTTCATTCTTCGGTAATGTTGAGAATTTATATTTTATTGTGTCTGGTTGAATTTTCATCTCATTTCTTGAATTTATATGTTAGTATTGCGCTATATTTATTCCCCCCAAAGCTGGCATTGTAGTAATGGTCAATTCTCCAATGAGATAGCCATTTTATGTTATGTTGTGGAAACAATCAGATGTTAATGAATCTGGCATTCATGGTGTGGGAGATCAAAATTTGAAATAACATATTATTAGATAATCTGGGAGATGACGAACTCAACCACTAAAACTCGTGTATCTCAGCAAAAAAGCTACTCATTATTATTAAAATTTTGCCTTCTTTCGTTTTGCGACAAACAACGAGGAGACGATGGGAACAAAAAAAGAAGAATCCATGAAAAAGAATTCAGTCAAAAGAAGAACCCGAGGAGGAGGAAGGACCGTCGTTTCTGCAACTGCGAACAACAGACAGGGAAGAAGGAGAAGGCGCGATCCGCCCGACCCGATCCGACTCGACTCGGTTGGAGAAAGATGGTGGTGGAGTGGCTGATTAAAACCGAAGATACTAATAAGGTGAAGTGGGGCAAGGAGGGGAGTAATAACCTCATTGAGTTTCTTGATGGGAGAGAA

mRNA sequence

GAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCTGCTTATTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTGATGATTTTCGCAAGTATGTCTTGTCATGTTCATCTTCTGAGGACTGCCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGACTGGAAGTGGCCATTTTTCCCCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCTCACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGAGTTGTCAGCATGACGGTTGGAATAACATTATAAAATACTTAACAGAAGAAATTCCCCAACTTTTGAAAACAGAAGAGGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTATGCAAAGGCTTGACATCTTCGTTTGACACAGATGAATTACATGCCATTGCTGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTGGGCTGATCGAAGTTGCTGCAAAACCGATATCCATCATCTGAAAGGTGAAAATGAGAAGGCAGCAGTGGTGGTGTCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAATCTTCCTCATCTTCTTCATATTTCATTCTTCGGTAATGTTGAGAATTTATATTTTATTGTGTCTGGTTGAATTTTCATCTCATTTCTTGAATTTATATGTTAGTATTGCGCTATATTTATTCCCCCCAAAGCTGGCATTGTAGTAATGGTCAATTCTCCAATGAGATAGCCATTTTATGTTATGTTGTGGAAACAATCAGATGTTAATGAATCTGGCATTCATGGTGTGGGAGATCAAAATTTGAAATAACATATTATTAGATAATCTGGGAGATGACGAACTCAACCACTAAAACTCGTGTATCTCAGCAAAAAAGCTACTCATTATTATTAAAATTTTGCCTTCTTTCGTTTTGCGACAAACAACGAGGAGACGATGGGAACAAAAAAAGAAGAATCCATGAAAAAGAATTCAGTCAAAAGAAGAACCCGAGGAGGAGGAAGGACCGTCGTTTCTGCAACTGCGAACAACAGACAGGGAAGAAGGAGAAGGCGCGATCCGCCCGACCCGATCCGACTCGACTCGGTTGGAGAAAGATGGTGGTGGAGTGGCTGATTAAAACCGAAGATACTAATAAGGTGAAGTGGGGCAAGGAGGGGAGTAATAACCTCATTGAGTTTCTTGATGGGAGAGAA

Coding sequence (CDS)

GAAGGGTTTTTCAAGTTGATTTCTTACTACCAAACGCAATCGGAACCTGCTTATTGTGGACTTGCCACCCTTGCCGTTGTCCTCAATGCTCTTTCTATTGATCCTGGTAGAAAATGGAAAGGTCCGTGGAGATGGTTTGATGACACTATGCTGGACTGCTGTGAACCTTTGGCAAAGATCAAAACTGATGGTATCACATTTGGCAAGGTTGCATGTTTGGCTCGCTGCAATGGTGCTAAAGTGGTAGCTTTTAGGGCAAATGAAAGCACCATTGATGATTTTCGCAAGTATGTCTTGTCATGTTCATCTTCTGAGGACTGCCATGTGATCACTTCCTACCATAGAGGAGCTTTTAAACAGACTGGAAGTGGCCATTTTTCCCCAATTGGGGGTTATCATACTGGAAAAGATATGGTTCTCATCTTGGATGTTGCGCGTTTCAAGTATCCTCCTCACTGGGTTCCTCTTACCCTACTGTGGGATGCCATGAACACAATTGACAGTGCAACAGGACTACCTAGAGGCTACATGATTTTATCAAAGCTTCCCAGGGGACCGTCTATTCTTTACACTCTGAGTTGTCAGCATGACGGTTGGAATAACATTATAAAATACTTAACAGAAGAAATTCCCCAACTTTTGAAAACAGAAGAGGTGAAAGATGTAAGGGGATTACTTTCTGTAGTATTTAAAATGCCACCTCCAAATCTCAGACAATTTATCAAGTGGGTTGCAGAAGTCCGGGAGAAAGAGGATGGGAATGTAAAACTAAATGCAGAGGAGAGAGGAAGACTAGCTGTCAAGGAAGAGATATTGGAACAACTCCGAGCGACTGAACTCTTCAAACATGTCAAACAATGGTTGGCATCTGAGACACTATGCAAAGGCTTGACATCTTCGTTTGACACAGATGAATTACATGCCATTGCTGCAAGTGTTTGTTGTCAAGGGGCAGAAACTTTAGGTGGGAAACGATGCTGGGCTGATCGAAGTTGCTGCAAAACCGATATCCATCATCTGAAAGGTGAAAATGAGAAGGCAGCAGTGGTGGTGTCAGGGAGCGTTGTTAGTAATGTTAGTACTAAACAAGGGGTAGATATGTTGGTACCTTTGTGTAAAACAGATGAGTGTTGTTGTGAGTGGCCATCTGTTGTTGATGTTCTAACCATATTGTTGCTATCACTGCCCCAACATATTTGGTCCACCGTTAAAGAAGAGAAGCTGTTGGCAGACATTAACACACTTGTTGCAGAAAAGAACCTTCCTGCTTTGCTTCAGGATGAGGTTCTGCACTTGAGACAACAAATGCACCTTCTTATGACTGATCTCGGCTCTCAATAA

Protein sequence

EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKIKTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQTGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILSKLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQFIKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSSFDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVSTKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKNLPALLQDEVLHLRQQMHLLMTDLGSQ
Homology
BLAST of Carg11547 vs. NCBI nr
Match: KAG7014605.1 (Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ
Sbjct: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF
Sbjct: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS
Sbjct: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 446

BLAST of Carg11547 vs. NCBI nr
Match: KAG6576090.1 (Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ
Sbjct: 98  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 158 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF
Sbjct: 218 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 278 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS
Sbjct: 338 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 397

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 398 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 457

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 458 LPALLQDEVLHLRQQMHLLMTDLGSQ 483

BLAST of Carg11547 vs. NCBI nr
Match: XP_023548579.1 (glutathione gamma-glutamylcysteinyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-259
Identity = 437/446 (97.98%), Postives = 442/446 (99.10%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVV+FRANESTIDDFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 98  KTDGITFGKVACLARCNGAKVVSFRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 158 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTE+VKDVR LLSVVFKMPPPNLRQF
Sbjct: 218 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEDVKDVRELLSVVFKMPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 278 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIAASVCCQGAETLGG++CWADRSCCKTDIHHL GENEKAAVVVSGSVVSNVS
Sbjct: 338 FDKDELHAIAASVCCQGAETLGGEQCWADRSCCKTDIHHLNGENEKAAVVVSGSVVSNVS 397

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 398 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 457

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPA LQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 458 LPASLQDEVLHLRQQMHLLMTDLGSQ 483

BLAST of Carg11547 vs. NCBI nr
Match: XP_022953840.1 (glutathione gamma-glutamylcysteinyltransferase 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 897.1 bits (2317), Expect = 6.1e-257
Identity = 434/446 (97.31%), Postives = 441/446 (98.88%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 2   EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 61

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTI+DFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 62  KTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQ 121

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 122 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 181

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWN++IKYLTEEIPQLLK E+VKDVRGLLSVVFKMPPPNLRQF
Sbjct: 182 KLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQF 241

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 242 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 301

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIAASVCCQGAETLGGKRC ADRSCCKTDIH L GENEKAAVVV+GSVVSNVS
Sbjct: 302 FDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVS 361

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 362 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 421

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 422 LPALLQDEVLHLRQQMHLLMTDLGSQ 447

BLAST of Carg11547 vs. NCBI nr
Match: XP_022953839.1 (glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 897.1 bits (2317), Expect = 6.1e-257
Identity = 434/446 (97.31%), Postives = 441/446 (98.88%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTI+DFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 98  KTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 158 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWN++IKYLTEEIPQLLK E+VKDVRGLLSVVFKMPPPNLRQF
Sbjct: 218 KLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 278 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIAASVCCQGAETLGGKRC ADRSCCKTDIH L GENEKAAVVV+GSVVSNVS
Sbjct: 338 FDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVS 397

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 398 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 457

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 458 LPALLQDEVLHLRQQMHLLMTDLGSQ 483

BLAST of Carg11547 vs. ExPASy Swiss-Prot
Match: Q2QKL5 (Glutathione gamma-glutamylcysteinyltransferase 3 OS=Lotus japonicus OX=34305 GN=PCS3 PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 5.5e-168
Identity = 281/433 (64.90%), Postives = 339/433 (78.29%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKL+SYYQTQSEPAYCGLATL VVLNALSIDPGRKWKGPWRWFDD+MLDCCEPL KI
Sbjct: 38  EGFFKLVSYYQTQSEPAYCGLATLTVVLNALSIDPGRKWKGPWRWFDDSMLDCCEPLEKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GITFGKVACLARCNGA V AFR+NEST+ DFR  V+SC SSED H+I SYHR   KQ
Sbjct: 98  KVQGITFGKVACLARCNGAHVEAFRSNESTVSDFRDRVISCCSSEDRHLIVSYHRSGLKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG GHFSPIGGYH  +DMVLILDV R+KYPPHWVPLTLLWDAMNTID ATGL RGYMI+S
Sbjct: 158 TGEGHFSPIGGYHAERDMVLILDVTRYKYPPHWVPLTLLWDAMNTIDRATGLQRGYMIIS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KL R PSILYT+SC+H+GW+++ K+LTE +P LLK+E++KD++ +LSVVFK PP  LR+F
Sbjct: 218 KLKRAPSILYTVSCRHEGWSSVAKFLTENVPLLLKSEDLKDIQEVLSVVFKSPPSELREF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASE-TLCKGLTS 300
           I W+AEVR +EDGN+ L+ EE+GRLA+K +ILEQ+R T LFKHV  WL S+ + C+ +  
Sbjct: 278 ITWIAEVRRQEDGNLTLSEEEKGRLAIKADILEQIRTTTLFKHVTSWLDSQRSRCRTIAK 337

Query: 301 SFDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNV 360
             D D L  +AA VCCQGA  L G      + C + D+ HL  +++    +VSG+V S  
Sbjct: 338 LQDRDMLPELAAGVCCQGACLLTGCCLPGGKCCSQIDVKHLNVDHKNIVTLVSGTVASGS 397

Query: 361 STKQGVDMLVPLCK---TDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLV 420
           S++QGVD+LVPLC+      C    PS  DVLT+LLL+LP H WS +KEEKL A++ +L+
Sbjct: 398 SSEQGVDVLVPLCQMGPEGHCIGMHPSTADVLTVLLLALPLHTWSGIKEEKLCAEVTSLL 457

Query: 421 AEKNLPALLQDEV 430
             +NLP LLQ+EV
Sbjct: 458 TTENLPPLLQEEV 470

BLAST of Carg11547 vs. ExPASy Swiss-Prot
Match: Q2TE74 (Glutathione gamma-glutamylcysteinyltransferase 2 OS=Lotus japonicus OX=34305 GN=PCS2 PE=2 SV=2)

HSP 1 Score: 578.6 bits (1490), Expect = 6.3e-164
Identity = 275/435 (63.22%), Postives = 339/435 (77.93%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLIS+YQTQSEPAYCGLATL+VVLNAL+IDPGRKWKGPWRWFDD+MLDCCEPL K+
Sbjct: 38  EGFFKLISFYQTQSEPAYCGLATLSVVLNALAIDPGRKWKGPWRWFDDSMLDCCEPLEKV 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GITFGKVACLARCNGAKV AFR+NEST+ DFR  V+SC SSED H I SYHR   KQ
Sbjct: 98  KVQGITFGKVACLARCNGAKVEAFRSNESTVSDFRNRVISCCSSEDRHAIVSYHRSGLKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG GHFSP+GGYH  +DMVLILDV R+KYPPHWVP+TLLW+AMNTID ATGL RGYMI+S
Sbjct: 158 TGEGHFSPLGGYHAERDMVLILDVTRYKYPPHWVPVTLLWNAMNTIDRATGLQRGYMIIS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KL R PSILYTLSC+H+GW+++ K+LTE +P LLK+E++KD++ +LSVVFK PP  LR+F
Sbjct: 218 KLNRAPSILYTLSCRHEGWSSVAKFLTENVPLLLKSEDLKDIQEVLSVVFKSPPSELREF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASE-TLCKGLTS 300
           I W+AEVR +EDGN+ L+ EE+GRL +K EILE++R   LFKHV  WL S+ + C  + +
Sbjct: 278 ITWIAEVRRQEDGNLTLSEEEKGRLVIKAEILEKIRTAGLFKHVTSWLDSQRSRCSTIAN 337

Query: 301 SFDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEK-AAVVVSGSVVSN 360
             D D L  +AA VCCQGA  L G      + CC+ D+ HL  +++  A   VSG+  + 
Sbjct: 338 LQDKDMLPELAARVCCQGACLLTGCCLSGGKCCCQIDVKHLNVDSKNLATTFVSGTFTTG 397

Query: 361 VSTKQGVDMLVPLCK---TDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTL 420
            S++QGVD+LVPLC+           PS  DVLT+LLL+LP H WS +KEEKL A++ +L
Sbjct: 398 SSSEQGVDVLVPLCQRGPEGHYIAMHPSTADVLTVLLLALPLHTWSGIKEEKLRAEVTSL 457

Query: 421 VAEKNLPALLQDEVL 431
           V  ++LP+LLQ+EV+
Sbjct: 458 VTTEDLPSLLQEEVI 472

BLAST of Carg11547 vs. ExPASy Swiss-Prot
Match: Q2TSC7 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Lotus japonicus OX=34305 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.1e-152
Identity = 265/450 (58.89%), Postives = 343/450 (76.22%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           E F++L+SY+QTQSEPA+CGLA+L++VLNAL+IDPGRKWKGPWRWFD++MLDCCEPL KI
Sbjct: 38  ESFYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLDKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GI+FGK+ CLA C GAKV AF A+ S+ID FRKYV+ CS+S+DCHVI+SYHR A KQ
Sbjct: 98  KARGISFGKLVCLAHCAGAKVEAFHASHSSIDHFRKYVMKCSTSDDCHVISSYHREALKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGYH GKDM LILDVARFKYPPHW+PLT LW+ MN +D +TG  RG+M++S
Sbjct: 158 TGTGHFSPIGGYHAGKDMALILDVARFKYPPHWIPLTHLWEGMNYVDESTGKTRGFMLIS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +  R P +LYTLSC+H+ WN+I K+L ++IP LL +E+VKD+  +LSV+    P N  +F
Sbjct: 218 RPHREPGMLYTLSCKHESWNSIAKFLIDDIPFLLTSEDVKDICKVLSVIVTSLPSNFEEF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLT-S 300
           IKWVAE+R  EDG+  L+ EE+ RL+VKEEIL+Q++ T LFKHV  +L+    C G T +
Sbjct: 278 IKWVAEIRRGEDGSPSLSVEEKARLSVKEEILKQVQRTGLFKHVASFLSHS--CSGHTPT 337

Query: 301 SFDTDELHAIAASVCCQGAETLGGKRCWADRSCCK-TDIHHLKGENEKAAVVVSGSVVSN 360
           S D D    IAASVCCQGAE LGGK   +   CC+ T +   K E++K   +V G+VV N
Sbjct: 338 SGDRDTFPVIAASVCCQGAEILGGKISSSAEYCCRETCMKCWKAEDDKPIRMVCGTVV-N 397

Query: 361 VSTKQGVDMLVP-LCKTDECCC--------EWPSVVDVLTILLLSLPQHIWSTVKEEKLL 420
            +T+QGVD+L+P  C    C C        + P+  DVLT+LLLSLP   W+ + +EKLL
Sbjct: 398 GNTEQGVDVLIPSSCGKLSCTCSSTTKSIRKHPASTDVLTVLLLSLPTSTWAGIADEKLL 457

Query: 421 ADINTLVAEKNLPALLQDEVLHLRQQMHLL 440
           ++I+ LV+ +NLPALLQ+EVLHLR+Q+H+L
Sbjct: 458 SEIHDLVSIENLPALLQEEVLHLRRQLHIL 484

BLAST of Carg11547 vs. ExPASy Swiss-Prot
Match: Q9S7Z3 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.2e-142
Identity = 247/444 (55.63%), Postives = 329/444 (74.10%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFF+LISY+QTQSEPAYCGLA+L+VVLNALSIDPGRKWKGPWRWFD++MLDCCEPL  +
Sbjct: 38  EGFFRLISYFQTQSEPAYCGLASLSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVV 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GI+FGKV CLA C+GAKV AFR ++STIDDFRK+V+ C+SSE+CH+I++YHRG FKQ
Sbjct: 98  KEKGISFGKVVCLAHCSGAKVEAFRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGY+  +DM LILDVARFKYPPHWVPL LLW+AM++ID +TG  RG+M++S
Sbjct: 158 TGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLIS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +  R P +LYTLSC+ + W  I KYL E++P+L+ ++ V  V  ++SVVFK  P N  QF
Sbjct: 218 RPHREPGLLYTLSCKDESWIEIAKYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           I+WVAE+R  ED N  L+AEE+ RL +K+ +L+++  TELFKH+ ++L+        T  
Sbjct: 278 IRWVAEIRITEDSNQNLSAEEKSRLKLKQLVLKEVHETELFKHINKFLS--------TVG 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCK-TDIHHLKGENEKAAVVVSGSVVSNV 360
           ++ D L   AA  CCQGAE L G    +   CC+ T +  +KG ++    VV+G VV + 
Sbjct: 338 YE-DSLTYAAAKACCQGAEILSGSP--SKEFCCRETCVKCIKGPDDSEGTVVTGVVVRD- 397

Query: 361 STKQGVDMLVPLCKTDECCC----EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTL 420
             +Q VD+LVP  +T EC C     +P+  DV T LLL+LP   WS +K++ L+ ++  L
Sbjct: 398 GNEQKVDLLVPSTQT-ECECGPEATYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQL 457

Query: 421 VAEKNLPALLQDEVLHLRQQMHLL 440
           ++  +LP LLQ+EVLHLR+Q+ LL
Sbjct: 458 ISMASLPTLLQEEVLHLRRQLQLL 468

BLAST of Carg11547 vs. ExPASy Swiss-Prot
Match: Q9SWW5 (Glutathione gamma-glutamylcysteinyltransferase 1 OS=Triticum aestivum OX=4565 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.6e-141
Identity = 246/449 (54.79%), Postives = 319/449 (71.05%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFF LISY+QTQSEPA+CGLA+L+VVLNAL+IDPGR WKGPWRWFD++MLDCCEPL K+
Sbjct: 38  EGFFNLISYFQTQSEPAFCGLASLSVVLNALAIDPGRPWKGPWRWFDESMLDCCEPLHKV 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K +GITFGKV CLA C GA+V +FRA+++TI DFR ++  C+SS+DCH+I+SYHR  FKQ
Sbjct: 98  KAEGITFGKVVCLAHCAGARVQSFRADQTTIHDFRAHLTRCASSQDCHLISSYHRSPFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGYH  KDM LILDVARFKYPPHWVPLTLLWDAMNT D ATGL RG+M++S
Sbjct: 158 TGTGHFSPIGGYHAEKDMALILDVARFKYPPHWVPLTLLWDAMNTTDEATGLLRGFMLVS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +    PS+LYT+SC H  W ++ KY  E++P LLK E + +V  LLS + +  P N    
Sbjct: 218 RRSSAPSLLYTVSCGHGSWKSMAKYCVEDVPNLLKDESLDNVTTLLSRLVESLPANAGDL 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IK V EVR KE+G   L+ EE+ RL +KE++L+Q+R T+LF+ V +    + LC   +SS
Sbjct: 278 IKCVIEVRRKEEGESSLSKEEKERLFLKEKVLQQIRDTDLFRVVHELQYPKGLCGSCSSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCK-TDIHHLKGENEKAAVVVSGSVVSNV 360
            D D L  IAA+VCCQGA  L G     D  CC+ T I  ++   +    V+SG+VVS  
Sbjct: 338 SDEDSLAEIAATVCCQGAAFLSGNLVSRDGFCCRETCIKCIEANGDGLKTVISGTVVSK- 397

Query: 361 STKQGVDMLVPLCKTDECCC---------EWPSVVDVLTILLLSLPQHIWSTVKEEKLLA 420
             +Q VD+L+P   +    C         ++PS  DVLT+LLL L  + W  +K+E + A
Sbjct: 398 GNEQAVDLLLPTSSSKTSLCNSNLKSKIVKYPSSTDVLTVLLLVLQPNTWLGIKDENVKA 457

Query: 421 DINTLVAEKNLPALLQDEVLHLRQQMHLL 440
           +  +LV+  NLP LL+ E+LHLR+Q+H L
Sbjct: 458 EFQSLVSTDNLPDLLKQEILHLRRQLHYL 485

BLAST of Carg11547 vs. ExPASy TrEMBL
Match: A0A6J1GQY9 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456253 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 2.9e-257
Identity = 434/446 (97.31%), Postives = 441/446 (98.88%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTI+DFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 98  KTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 158 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWN++IKYLTEEIPQLLK E+VKDVRGLLSVVFKMPPPNLRQF
Sbjct: 218 KLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 278 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIAASVCCQGAETLGGKRC ADRSCCKTDIH L GENEKAAVVV+GSVVSNVS
Sbjct: 338 FDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVS 397

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 398 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 457

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 458 LPALLQDEVLHLRQQMHLLMTDLGSQ 483

BLAST of Carg11547 vs. ExPASy TrEMBL
Match: A0A6J1GQS9 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456253 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 2.9e-257
Identity = 434/446 (97.31%), Postives = 441/446 (98.88%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 2   EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 61

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTI+DFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 62  KTDGITFGKVACLARCNGAKVVAFRANESTINDFRKYVVSCSSSEDCHVITSYHRGAFKQ 121

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 122 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 181

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWN++IKYLTEEIPQLLK E+VKDVRGLLSVVFKMPPPNLRQF
Sbjct: 182 KLPRGPSILYTLSCQHDGWNSVIKYLTEEIPQLLKAEDVKDVRGLLSVVFKMPPPNLRQF 241

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 242 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 301

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIAASVCCQGAETLGGKRC ADRSCCKTDIH L GENEKAAVVV+GSVVSNVS
Sbjct: 302 FDKDELHAIAASVCCQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVAGSVVSNVS 361

Query: 361 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 420
           TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN
Sbjct: 362 TKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEKN 421

Query: 421 LPALLQDEVLHLRQQMHLLMTDLGSQ 447
           LPALLQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 422 LPALLQDEVLHLRQQMHLLMTDLGSQ 447

BLAST of Carg11547 vs. ExPASy TrEMBL
Match: A0A6J1JSE5 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488492 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.2e-252
Identity = 431/447 (96.42%), Postives = 436/447 (97.54%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 2   EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 61

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 62  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQ 121

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 122 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 181

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWNN+IKYLTEEIPQLLKTE+VKDV  LLSVVFKMPPPNLRQF
Sbjct: 182 KLPRGPSILYTLSCQHDGWNNVIKYLTEEIPQLLKTEDVKDVGELLSVVFKMPPPNLRQF 241

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVRE+EDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 242 IKWVAEVREQEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 301

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIA SVC QGAETLGGKRC ADRSCCKTDIH L GENEKAAVVVSGSVVSNVS
Sbjct: 302 FDKDELHAIALSVCSQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVSGSVVSNVS 361

Query: 361 TKQG-VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 420
           TKQG VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK
Sbjct: 362 TKQGVVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 421

Query: 421 NLPALLQDEVLHLRQQMHLLMTDLGSQ 447
           NL A LQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 422 NLSASLQDEVLHLRQQMHLLMTDLGSQ 448

BLAST of Carg11547 vs. ExPASy TrEMBL
Match: A0A6J1JUJ2 (Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488492 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.2e-252
Identity = 431/447 (96.42%), Postives = 436/447 (97.54%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPA+CGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAFCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYV+SCSSSEDCHVITSYHRGAFKQ
Sbjct: 98  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVVSCSSSEDCHVITSYHRGAFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS
Sbjct: 158 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           KLPRGPSILYTLSCQHDGWNN+IKYLTEEIPQLLKTE+VKDV  LLSVVFKMPPPNLRQF
Sbjct: 218 KLPRGPSILYTLSCQHDGWNNVIKYLTEEIPQLLKTEDVKDVGELLSVVFKMPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVRE+EDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS
Sbjct: 278 IKWVAEVREQEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           FD DELHAIA SVC QGAETLGGKRC ADRSCCKTDIH L GENEKAAVVVSGSVVSNVS
Sbjct: 338 FDKDELHAIALSVCSQGAETLGGKRCSADRSCCKTDIHLLNGENEKAAVVVSGSVVSNVS 397

Query: 361 TKQG-VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 420
           TKQG VDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK
Sbjct: 398 TKQGVVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 457

Query: 421 NLPALLQDEVLHLRQQMHLLMTDLGSQ 447
           NL A LQDEVLHLRQQMHLLMTDLGSQ
Sbjct: 458 NLSASLQDEVLHLRQQMHLLMTDLGSQ 484

BLAST of Carg11547 vs. ExPASy TrEMBL
Match: A0A6J1DUP4 (Glutathione gamma-glutamylcysteinyltransferase OS=Momordica charantia OX=3673 GN=LOC111024196 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.4e-229
Identity = 388/451 (86.03%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFFKLISYYQTQSEPAYCGL TLAVVLNALSIDPGRKWKGPWRWFDD+MLDCCEPLAKI
Sbjct: 38  EGFFKLISYYQTQSEPAYCGLGTLAVVLNALSIDPGRKWKGPWRWFDDSMLDCCEPLAKI 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           KT+GITFGKVACLARCNGAKVVAFR NESTIDDFRKYV+SCSSSEDCHVITSYHRG FKQ
Sbjct: 98  KTEGITFGKVACLARCNGAKVVAFRTNESTIDDFRKYVVSCSSSEDCHVITSYHRGVFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGYH GKDMVLILDVARFKYPPHWVPLTLLWDAMNTID ATGLPRGYMILS
Sbjct: 158 TGTGHFSPIGGYHAGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDGATGLPRGYMILS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +LPRGPSILYTLSCQH+GWNN+IKYLTEE+P LLKTE+VKDV+ LL+VVFK+PPPNLRQF
Sbjct: 218 RLPRGPSILYTLSCQHEGWNNVIKYLTEEVPLLLKTEDVKDVQELLTVVFKLPPPNLRQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKWVAEVRE+EDGNVKL+ EERGRLAVKEEILEQLR TELFKH+KQWLAS TLCKGL S 
Sbjct: 278 IKWVAEVREQEDGNVKLSVEERGRLAVKEEILEQLRETELFKHIKQWLASGTLCKGLASL 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCKTDIHHLKGENEKAAVVVSGSVVSNVS 360
           F+ DEL  IAA+VCCQGAETL GK C ADR+ CKTDIH L GENEK AVVVSG+VVS V 
Sbjct: 338 FNKDELPEIAATVCCQGAETLAGKSCSADRTFCKTDIHLLNGENEKPAVVVSGTVVSKV- 397

Query: 361 TKQGVDMLVPLCKT--------DECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADI 420
           TKQGVDMLVPLCKT        DECCC+WPSV+DVLTILLLSLPQHIWST+KEEKLL DI
Sbjct: 398 TKQGVDMLVPLCKTESSQSQISDECCCDWPSVIDVLTILLLSLPQHIWSTIKEEKLLGDI 457

Query: 421 NTLVAEKNLPALLQDEVLHLRQQMHLLMTDL 444
             LVAE  LPA+L++EVLHLRQQMH LMTDL
Sbjct: 458 KRLVAEDYLPAMLREEVLHLRQQMHFLMTDL 487

BLAST of Carg11547 vs. TAIR 10
Match: AT5G44070.1 (phytochelatin synthase 1 (PCS1) )

HSP 1 Score: 505.8 bits (1301), Expect = 3.7e-143
Identity = 247/444 (55.63%), Postives = 329/444 (74.10%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFF+LISY+QTQSEPAYCGLA+L+VVLNALSIDPGRKWKGPWRWFD++MLDCCEPL  +
Sbjct: 38  EGFFRLISYFQTQSEPAYCGLASLSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVV 97

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GI+FGKV CLA C+GAKV AFR ++STIDDFRK+V+ C+SSE+CH+I++YHRG FKQ
Sbjct: 98  KEKGISFGKVVCLAHCSGAKVEAFRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQ 157

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGY+  +DM LILDVARFKYPPHWVPL LLW+AM++ID +TG  RG+M++S
Sbjct: 158 TGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLIS 217

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +  R P +LYTLSC+ + W  I KYL E++P+L+ ++ V  V  ++SVVFK  P N  QF
Sbjct: 218 RPHREPGLLYTLSCKDESWIEIAKYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQF 277

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           I+WVAE+R  ED N  L+AEE+ RL +K+ +L+++  TELFKH+ ++L+        T  
Sbjct: 278 IRWVAEIRITEDSNQNLSAEEKSRLKLKQLVLKEVHETELFKHINKFLS--------TVG 337

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCK-TDIHHLKGENEKAAVVVSGSVVSNV 360
           ++ D L   AA  CCQGAE L G    +   CC+ T +  +KG ++    VV+G VV + 
Sbjct: 338 YE-DSLTYAAAKACCQGAEILSGSP--SKEFCCRETCVKCIKGPDDSEGTVVTGVVVRD- 397

Query: 361 STKQGVDMLVPLCKTDECCC----EWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTL 420
             +Q VD+LVP  +T EC C     +P+  DV T LLL+LP   WS +K++ L+ ++  L
Sbjct: 398 GNEQKVDLLVPSTQT-ECECGPEATYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQL 457

Query: 421 VAEKNLPALLQDEVLHLRQQMHLL 440
           ++  +LP LLQ+EVLHLR+Q+ LL
Sbjct: 458 ISMASLPTLLQEEVLHLRRQLQLL 468

BLAST of Carg11547 vs. TAIR 10
Match: AT1G03980.1 (phytochelatin synthase 2 )

HSP 1 Score: 469.9 bits (1208), Expect = 2.2e-132
Identity = 232/440 (52.73%), Postives = 307/440 (69.77%), Query Frame = 0

Query: 1   EGFFKLISYYQTQSEPAYCGLATLAVVLNALSIDPGRKWKGPWRWFDDTMLDCCEPLAKI 60
           EGFF LISY+QTQSEPA+CGLA+L++VLN+LSIDPGRKWKGPWRWFD++ML+CCEPL  +
Sbjct: 37  EGFFGLISYFQTQSEPAFCGLASLSMVLNSLSIDPGRKWKGPWRWFDESMLECCEPLEIV 96

Query: 61  KTDGITFGKVACLARCNGAKVVAFRANESTIDDFRKYVLSCSSSEDCHVITSYHRGAFKQ 120
           K  GI+FGKV CLA  +GAKV AFR N+STIDDFRKYV+ CS+S++CH+I++YHR   KQ
Sbjct: 97  KDKGISFGKVVCLAHSSGAKVEAFRTNQSTIDDFRKYVVKCSTSDNCHMISTYHRQVLKQ 156

Query: 121 TGSGHFSPIGGYHTGKDMVLILDVARFKYPPHWVPLTLLWDAMNTIDSATGLPRGYMILS 180
           TG+GHFSPIGGY+  +DM LILDVARFKYPPHWVPL LLWDAM++ID +TG  RG+M++S
Sbjct: 157 TGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWDAMDSIDQSTGRRRGFMLIS 216

Query: 181 KLPRGPSILYTLSCQHDGWNNIIKYLTEEIPQLLKTEEVKDVRGLLSVVFKMPPPNLRQF 240
           +  R P +LYTLSC+ + W +I KYL E++P+L+ ++ V  +  +L VVFK  P N  QF
Sbjct: 217 RPHREPGLLYTLSCKDESWISIAKYLKEDVPRLVSSQHVDTIERILYVVFKSLPANFNQF 276

Query: 241 IKWVAEVREKEDGNVKLNAEERGRLAVKEEILEQLRATELFKHVKQWLASETLCKGLTSS 300
           IKW+AE+R  ED N  L++EE+ RL +K+E+L+Q++ T+LFKHV ++L          SS
Sbjct: 277 IKWMAEIRRTEDVNQNLSSEEKSRLKLKQELLKQVQETKLFKHVDKFL----------SS 336

Query: 301 FDTDELHAIAASVCCQGAETLGGKRCWADRSCCK-TDIHHLKGENEKAAVVVSGSVVSNV 360
              D L  +AA V C G E L G    +D SCCK T +  +KG  E+   VV+       
Sbjct: 337 VYEDNLPYVAAKVYCDGDEILSGYE--SDESCCKETCVKCIKGLGEEKVTVVA------- 396

Query: 361 STKQGVDMLVPLCKTDECCCEWPSVVDVLTILLLSLPQHIWSTVKEEKLLADINTLVAEK 420
                                +PS  DV T LLL+LP   WS +K++ LL ++  L++  
Sbjct: 397 ---------------------YPSGNDVFTALLLALPPQTWSGIKDQSLLQEMKQLISMV 436

Query: 421 NLPALLQDEVLHLRQQMHLL 440
           + P LLQ EVLHLR+Q+ +L
Sbjct: 457 SHPTLLQQEVLHLRRQLEML 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014605.11.8e-264100.00Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperm... [more]
KAG6576090.11.8e-264100.00Glutathione gamma-glutamylcysteinyltransferase 3, partial [Cucurbita argyrosperm... [more]
XP_023548579.11.3e-25997.98glutathione gamma-glutamylcysteinyltransferase 3-like [Cucurbita pepo subsp. pep... [more]
XP_022953840.16.1e-25797.31glutathione gamma-glutamylcysteinyltransferase 3-like isoform X2 [Cucurbita mosc... [more]
XP_022953839.16.1e-25797.31glutathione gamma-glutamylcysteinyltransferase 3-like isoform X1 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
Q2QKL55.5e-16864.90Glutathione gamma-glutamylcysteinyltransferase 3 OS=Lotus japonicus OX=34305 GN=... [more]
Q2TE746.3e-16463.22Glutathione gamma-glutamylcysteinyltransferase 2 OS=Lotus japonicus OX=34305 GN=... [more]
Q2TSC71.1e-15258.89Glutathione gamma-glutamylcysteinyltransferase 1 OS=Lotus japonicus OX=34305 GN=... [more]
Q9S7Z35.2e-14255.63Glutathione gamma-glutamylcysteinyltransferase 1 OS=Arabidopsis thaliana OX=3702... [more]
Q9SWW52.6e-14154.79Glutathione gamma-glutamylcysteinyltransferase 1 OS=Triticum aestivum OX=4565 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1GQY92.9e-25797.31Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1GQS92.9e-25797.31Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JSE52.2e-25296.42Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1JUJ22.2e-25296.42Glutathione gamma-glutamylcysteinyltransferase OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1DUP44.4e-22986.03Glutathione gamma-glutamylcysteinyltransferase OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT5G44070.13.7e-14355.63phytochelatin synthase 1 (PCS1) [more]
AT1G03980.12.2e-13252.73phytochelatin synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038156Phytochelatin synthase, N-terminal domain superfamilyGENE3D3.90.70.30coord: 1..182
e-value: 8.4E-86
score: 288.6
IPR007719Phytochelatin synthase, N-terminal catalytic domainPFAMPF05023Phytochelatincoord: 1..176
e-value: 3.0E-76
score: 255.3
IPR007719Phytochelatin synthase, N-terminal catalytic domainPROSITEPS51443PCScoord: 1..184
score: 43.632595
IPR015407Phytochelatin synthase, C-terminalPFAMPF09328Phytochelatin_Ccoord: 194..436
e-value: 1.2E-87
score: 293.6
NoneNo IPR availablePANTHERPTHR33447:SF10GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASEcoord: 1..441
IPR040409Phytochelatin synthasePANTHERPTHR33447GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASEcoord: 1..441
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 2..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11547-RACarg11547-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098849 cellular detoxification of cadmium ion
biological_process GO:0010273 detoxification of copper ion
biological_process GO:0046938 phytochelatin biosynthetic process
biological_process GO:0010038 response to metal ion
molecular_function GO:0016756 glutathione gamma-glutamylcysteinyltransferase activity
molecular_function GO:0046872 metal ion binding