Carg11533 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11533
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionS-(hydroxymethyl)glutathione dehydrogenase
LocationCarg_Chr17: 9581163 .. 9587541 (+)
RNA-Seq ExpressionCarg11533
SyntenyCarg11533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTTCTCTCTAGCCTCTCATTCTTCTTTCTCGATCTCTTTCACTGTGTTCAGTTCTTCTATCCATGGCGACTCAAGGTCAAGTTATCACCTGCAAAGGTACTCCCTATACACATTTTATTCTAACTCTATATGCTTCACTTTTTCCTGCCATCGATCCTTTTGATTCAGCTAGCGTTTTTTCTTTTTCTTTTTCTGTCAGCGGCGGTGGCCTGGGAACCCAACAAGCCATTGGTGATCGAGGATGTTCAGGTGGCTCCGCCACAAGCCGGCGAGGTCCGAGTCAAGATCCTCTACACTGCTCTTTGTCACACTGATGCTTATACCTGGAGCGGCAAGGTTGTTTATCTTTTGATTTTCCGAATCTTAATTGATTGAATTGTCGGTCATTGCATGGCGCTTCTTTTTTCTTAATTTCTCTACTATTTTTTTGGTGATTCATTTTGGGGGTGTGCTTTCGCTCTTTGAAGAGTTCGTGATTTTTTAGTCCCTTTCTATTGGGTGGTGGAATGAAATTGAATTCGCAGTTCTTGTGCATTGATGATTGTAATTCTATCTTCAGATTAGGAAATACTAATTTCTGCGTGAATTATTTTATTCGAGTTTGCAAGATTGTCCAATCGGACAAGGTTGGTTTACTTCTCCAAAGACAAAATTTGTATGGTTAGGATCTATTATGTTCTTACTAATTTTTCTTTGTTCTATATCGATAAATTCAACGCGTTAACACCAGTGCTGGAATCTTCACGAGGATCCTGGATCAGACGGGATCTGGTGCGCTACCAATATGCTCTGTGGCATTTAGATAACTGAAAATCTAATTTCCAAGATCCTAGCTGTAATGGTTACTGAAAAAAAGCAACCTCCCTTCTAATCAATAGGTTTAGATAACTACCATTTTTCTTTCTCGAAGGACACTTCGACTGCATTGTACCAACTAAGTTAGCCTTGAACTCGAAGGGGTGATCACACTACAGATACAGCTAAAAGTCCTTTTTGAGATTGTGGAATTACCCAACCAAACTGAAGAAAGAAAACACGTTTTGTTCCCATGTTTGCTATGCATTGAATATGCAGAAACACCTTTTGGGGAAATAGACCCGCTTTGGAATTTCTTCTTTCAATTTTGTAAAAAATGTTTAAATCTTTTGGAGAACTTCCAAACTTAATTTTTGTTAGAACCCTTCTGGTTCCAAATTGATCTGGGCGAAGGATTCCTCCACTTCTATATTAGTGATAAAGTTGAAATAAATAATTTATCATATTAACTGATGTATAACATATTTTTATTGGATTTTTTTTAATCTCTTTTTATTCAATGAAAAAAAGAAGAGAATTCTTATTCCAGATTTCCAATACCTAAACTCTCATGAAGAAAAAGGTTTTTGGAGAAAACGTCCCATTTGATAAGCGGACAACCCAACTTCATGTTACTGGCCTAAAGTTTCTTCTTTTTTGCTCGCTCCTAAAATCCTAATAGTCTTGGCTGGATTTGGTTCTTTGCTTTGCTTACAGCCTTAGGATAGATTAATACTGAAGACATTTATGTCGAGAGCCTCCTTTTTTGTCCTTGGTCTGAACTGGATCCTCCTTTGTGAAGGAAGTTCTTGTTTTCTGGTAGGCTGGATTCTTTGTTATTTTGTGGTTTATTTGGTGGGAAGAAAATAGGAAAACATTTTTGGGGTTTGGTAGATAGTGGGAAGATATATGGCCACATTTTAATGCATCACTTTCGGATTTTAACTTAAATTGGTTTTGTAACTACCCTTTGTTATTTATTACCAAATGTTGGGGTTCTTTTTTGCAAGACTTTTTTGCACTTCCCACTTTTGGCTTGATATTTATTTTTGTGATGGGCTGTTCGTTGTACTACCCTTCATTTTATTGAAAGCTGGTTTCTCTTAACAAAATCGAGGATTAACAATTGTCTCTCATTTTTCAGTCATGTATAATATTACAATATGTGGCAAGTTTTGAACCTCCTATTAATTGTCCAATTGATTCTTTGGTTTAAGTGGCAGTTAAAGTTGTGTTTGACAAATCTACTTTTGGCACGTGCTATGACAAATTGAGGAGTTTCTAGTATAAAGGATCAATGAAGTCAATCACTTTGATAACCAAGATGTTTTTAGAATTTTTATTTTTTATTTTTATTTTTTTTATGTATTTTAGATAAATAACAAATTCATTGATGAATGAAATATGAAAGAGAGGAAAAAAAACCAAGCCCGAACACTTCGATCTAAGACATCATTAGCTCAATACCTTTCCTGGCCATTTGGCACCTGCCTAAAGTTTAATTTTCTCTTATTAATATTATTATTATCATTTCCTGTGCATGTTGAAACCCAACCTCCTGATATCTGACTTTTTTTAACTTTCATCTCTTTCTAGGACCCTGAAGGTCTCTTCCCCTGTATTCTTGGTCATGAGGCTGCAGGGTAGGCAACTTGATATCACATGCCAGATATTTTGGTTTTCATTATATATATAAATTTCACTACTTAAGCGGCAATATGAAACCGATGTGCAGGATAGTAGAGAGTGTTGGTGAAGGTGTGACGGAAGTTCAGCCTGGAGACCATGTAATTCCTTGCTACCAAGCAGAATGTCGAGAATGCAAGTTTTGCAAATCAGGGAAAACAAATCTTTGTGGCAAAGTCCGTGCTGCAACTGGAGTGGGAGTGATGATGTCAGATCGTCAGAGTCGTTTCTCCATAAATGGAAAACCTATATATCACTTCATGGGCACGTCAACATTTAGTCAATACACTGTTGTTCATGATGTCAGTGTTGCAAAGATCGATCCCACGGCTCCATTGGATAAAGTATGCCTTCTTGGATGTGGTGTTCCCACAGGTAGCGATATGTTAAACTTGCATCTATAATGTTTTATATATATATATATATATAAACCACCATGGGTTGATCTAGTGGTCAATAAGGATCAGACAAATAATAAAGGGTTTAGAGAGAGAGTGGATTCAAGTCATGGTAGCCAGGATAGAATATCCTGTGAATTACTTTGACTACCAAATATAGTAACGTCTGGCGGTCTTCTCTCGAGAATAGTTAAGGTGCACGTAAGGTTTCTTAAACACTCTTAATATAGACATATACGTTCATACATACATCCACACTTTTACGTTTACAACTAGCATGATTTTCCTGTTTTAACTATGAAAAATTAGAAAAAAGAAGATTTTAGATATAGGTGTATTTGTTTGTTAAAAGTTTATTTGGATGTCAAGGCGATGTTTTCTATCCAAGGGGAAATTTAAAAAATTCAGCTGACGTTCAATTTTCTTTTTGTTTCTCTAATTTATTCCTTTCTTTTTGAATGAGGGGGAGCTGTTCACATAAAATAATATCTGCCAACATAACTTTTTTTTTTTTTTTTTTTTCCTTTTCCATTTCACAATTCTTTATATGCCATAACGAATGGTATTTTCAGGCCTTGGAGCTGTTTGGAACACAGCAAAAGTAGAGCCAGGGTCCAATGTGGCTATTTTTGGCCTAGGGACTGTAGGCCTTGCGGTAAGCTCCATAGCAGACCCTCCTGTCTTTTGACTGCCTTCTCTTTCCTCTAAATTGACCTTTTATAGACTTAATGTTTGCTTGTAGTTATAGGTTAAAGGCAGATGGGCCTCACGTGATAGCAATAAGAATATTGTTAACTAATATTTTGGAATATAAAAGATATCTAATGCACGTAGCTATATAATACTCATATTACTTTACCACATGTTAAGAATAAACGCAAATGACTAAAATTGTAGAATATGCTGATCCTACGTGTGATACTGATTATATGTTAAAAAATAGAATCTTTATTAAAAATCACAATTGATACTAGGGACAAATCTTTCGCTCGATCATCCTTAAGATGGATGCGATGGGAAGAATTGGCATTTGGATGAGTTTTTTCCCCTATTAGCTTTTAAATATTGTTAACACAGTTATATTGGGGTTTTGCATCTTTTTAATGTTATTTATTAGCTTTTGTCTATATATCAAAGCTTATGCATTGTCTCTTTAAGCAAAGCCAGACAGCCTTTAGCCTTCTAAAAAATGTTTGCAGTGGTTTGATTGCTGCTAGCCATTTCTTCATGAACCCCAAAAATATTACCCAATCCTTTTCATAGTTATATCTTTTCTTTTTATCAGCGAAATTGTTTCTTTGGCTAATAGCATCAACTTCATTGTTACCTTAAATTTGACAGGTGGCAGAAGGTGCCAAAGCTGCTGGTGCCTCACGTATAATCGGCATTGATATTGACAGCAAAAAGTTTGAACTTGGTATACCTTGATTTCTCACATTTTATGCTCTATTTCTTGAATTTATAATCTAAAGGAGAGTCGTTCTAAAGCATATTATAATCTTTGAGGTTTGTGGTTTTATTATTATTATTTTTTCATGGCCATATTTTGTAACTGTTTTGCGACCTCAATGTATTTTCTAGATTTATAAATTTGGGGAGATCTGGTCGTTTGAACATAACTGAACTTTTTTACTGCTAATTCTTCACTAAATTTTTTTTGACATATATGTCGTCTGGAAAATTTTGGGATGTTTGTTTTCTTGATCTTTACTATTTTATGATCTTGAGGATAGACTTTGTTTGGAGTAGCAAAATGAGCTTTTATTGAGAAAATTTGAAGGAGTGGTGTTTTGTTCATTTGGCTCGATTTCTTTTCATAAAAGTTTGTTTGTATTAAAAAATTTCATTCGGCTTGAGAGCAGCGTGGGTTCTTGAACACTTTATTTTAAACTTTTTTCATCTCTTTAATTTTTACTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTAGTGTAAATTGTGTTCTCCTTTTCAATGCCTTAGCTTTACCTTTTCTATGGCAATTGGATGACCTTTTCGGTATCTTTCTTGGCTTGGGTTTTTTATTATTATTTTTTTTTTATCATCCTCTGTCTTTTGCAATTTCATTCTATCAATGAAATCAGCCTCTCCTACAAGAAAATTTATATTTTCTTTATTTTTATTTGATTTAAGTGGTAACTTTCCCTGGCCTTTTTAACGCCCAAATCTGCTGTTTGGGAGATACATATCCAATCAAACAGAACATACTCGTCTTTATGTATAACGAAACTAACAGTGTAGGACAAGATTTTCTCTATTTCCAATGATTCTTGAGGGGGCAATGAGGTGTGTTTATGACATTATTTACTGACACCTTTGCAGCCAAGAAATTTGGAGCGAATGAATTTGTGAACCCAAAGGAACATGACAAACCAATTCAGCAGGTCATCGTTGATCTCACAGATGGTGGCGTGGACTATAGTTTTGAGTGCATAGGGAATGTCAATGTGATGAGGTCTGCTCTGGAATGCTGCCACAAGGTAAGTGCTATAACAGATTTTATTGACTTGATAGATCATTGAAGTGGCTAACGTTCTGCCTGAGTTCTTTATGCATCTACTTACCAGGGCTGGGGGCAATCGGTCATTGTGGGAGTTGCAGCATCTGGCCAGGAAATATCCACAAGGCCTTTCCAGTTAGTGACTGGTCGCGTCTGGAAAGGAACAGCTTTTGGTGGGTTCAAGAGTCGTTCCCAAGTCCCTTGGCTCGTTGAAAAGTACATGAAGAAGGTATTTCTAGATGCTATCTTGTAGCTTGATTGTTATCTTTTAGAAAAAGAAATCTGCTATTGTTCTTGGTTGTTTATTGCACTTTCTAACTAAGACTTTAACATTTGATTTAGATGTTAACTATATTTTAAAAGGTTATAATACACGTTTAGTACTTGAGCTTTTAAGAAATTCAATTTTGTGTTACATAGATCTTAGTTTTCATGTTTCCAGGATGCTCACCAGTAATTTATCCATATGCTTGCAGGAAATCAAAGTTGACGAATACATAACGCACAACCTGACGCTGGAAGAGATCAACAAAGCCTTTGATCTGATGCATGGAGGCGATTGCCTGCGCTGCGTTCTTTCGGCCCATCCCTAAGCTCTAGCGCAAATTCATCTGTTCTTCCTTATGTAAGTTCCTAAAATAATGATGTGTTGAAATCTGACCACATATGCAATGATCCATAATGTTTTTGATATTTGAATTTGGATGGTATTGGTGATGATGAAAAGTGAACATGATATTAGTGTAGAGAACTTGGTGGATCCTTGAAGTTGTTTGATTCTGTGAATGCTGCAGTCAGTTCCTCCATCACTTTCATTCTTTAAGACCTCTTTTCTCACATGTGGAAGGGGTGGGCCAAGATTGTAACTACACAAAGGAGATATAATTATCACCTACAAACTAACATTTCCTGCTCTTTGTTTTGTTTTTGGGTAAAACTTCAAATACATTTAAAGCAGTTTGTT

mRNA sequence

CTTTTTTCTCTCTAGCCTCTCATTCTTCTTTCTCGATCTCTTTCACTGTGTTCAGTTCTTCTATCCATGGCGACTCAAGGTCAAGTTATCACCTGCAAAGCGGCGGTGGCCTGGGAACCCAACAAGCCATTGGTGATCGAGGATGTTCAGGTGGCTCCGCCACAAGCCGGCGAGGTCCGAGTCAAGATCCTCTACACTGCTCTTTGTCACACTGATGCTTATACCTGGAGCGGCAAGGACCCTGAAGGTCTCTTCCCCTGTATTCTTGGTCATGAGGCTGCAGGGATAGTAGAGAGTGTTGGTGAAGGTGTGACGGAAGTTCAGCCTGGAGACCATGTAATTCCTTGCTACCAAGCAGAATGTCGAGAATGCAAGTTTTGCAAATCAGGGAAAACAAATCTTTGTGGCAAAGTCCGTGCTGCAACTGGAGTGGGAGTGATGATGTCAGATCGTCAGAGTCGTTTCTCCATAAATGGAAAACCTATATATCACTTCATGGGCACGTCAACATTTAGTCAATACACTGTTGTTCATGATGTCAGTGTTGCAAAGATCGATCCCACGGCTCCATTGGATAAAGTATGCCTTCTTGGATGTGGTGTTCCCACAGGCCTTGGAGCTGTTTGGAACACAGCAAAAGTAGAGCCAGGGTCCAATGTGGCTATTTTTGGCCTAGGGACTGTAGGCCTTGCGGTGGCAGAAGGTGCCAAAGCTGCTGGTGCCTCACGTATAATCGGCATTGATATTGACAGCAAAAAGTTTGAACTTGCCAAGAAATTTGGAGCGAATGAATTTGTGAACCCAAAGGAACATGACAAACCAATTCAGCAGGTCATCGTTGATCTCACAGATGGTGGCGTGGACTATAGTTTTGAGTGCATAGGGAATGTCAATGTGATGAGGTCTGCTCTGGAATGCTGCCACAAGGGCTGGGGGCAATCGGTCATTGTGGGAGTTGCAGCATCTGGCCAGGAAATATCCACAAGGCCTTTCCAGTTAGTGACTGGTCGCGTCTGGAAAGGAACAGCTTTTGGTGGGTTCAAGAGTCGTTCCCAAGTCCCTTGGCTCGTTGAAAAGTACATGAAGAAGGAAATCAAAGTTGACGAATACATAACGCACAACCTGACGCTGGAAGAGATCAACAAAGCCTTTGATCTGATGCATGGAGGCGATTGCCTGCGCTGCGTTCTTTCGGCCCATCCCTAAGCTCTAGCGCAAATTCATCTGTTCTTCCTTATGTAAGTTCCTAAAATAATGATGTGTTGAAATCTGACCACATATGCAATGATCCATAATGTTTTTGATATTTGAATTTGGATGGTATTGGTGATGATGAAAAGTGAACATGATATTAGTGTAGAGAACTTGGTGGATCCTTGAAGTTGTTTGATTCTGTGAATGCTGCAGTCAGTTCCTCCATCACTTTCATTCTTTAAGACCTCTTTTCTCACATGTGGAAGGGGTGGGCCAAGATTGTAACTACACAAAGGAGATATAATTATCACCTACAAACTAACATTTCCTGCTCTTTGTTTTGTTTTTGGGTAAAACTTCAAATACATTTAAAGCAGTTTGTT

Coding sequence (CDS)

ATGGCGACTCAAGGTCAAGTTATCACCTGCAAAGCGGCGGTGGCCTGGGAACCCAACAAGCCATTGGTGATCGAGGATGTTCAGGTGGCTCCGCCACAAGCCGGCGAGGTCCGAGTCAAGATCCTCTACACTGCTCTTTGTCACACTGATGCTTATACCTGGAGCGGCAAGGACCCTGAAGGTCTCTTCCCCTGTATTCTTGGTCATGAGGCTGCAGGGATAGTAGAGAGTGTTGGTGAAGGTGTGACGGAAGTTCAGCCTGGAGACCATGTAATTCCTTGCTACCAAGCAGAATGTCGAGAATGCAAGTTTTGCAAATCAGGGAAAACAAATCTTTGTGGCAAAGTCCGTGCTGCAACTGGAGTGGGAGTGATGATGTCAGATCGTCAGAGTCGTTTCTCCATAAATGGAAAACCTATATATCACTTCATGGGCACGTCAACATTTAGTCAATACACTGTTGTTCATGATGTCAGTGTTGCAAAGATCGATCCCACGGCTCCATTGGATAAAGTATGCCTTCTTGGATGTGGTGTTCCCACAGGCCTTGGAGCTGTTTGGAACACAGCAAAAGTAGAGCCAGGGTCCAATGTGGCTATTTTTGGCCTAGGGACTGTAGGCCTTGCGGTGGCAGAAGGTGCCAAAGCTGCTGGTGCCTCACGTATAATCGGCATTGATATTGACAGCAAAAAGTTTGAACTTGCCAAGAAATTTGGAGCGAATGAATTTGTGAACCCAAAGGAACATGACAAACCAATTCAGCAGGTCATCGTTGATCTCACAGATGGTGGCGTGGACTATAGTTTTGAGTGCATAGGGAATGTCAATGTGATGAGGTCTGCTCTGGAATGCTGCCACAAGGGCTGGGGGCAATCGGTCATTGTGGGAGTTGCAGCATCTGGCCAGGAAATATCCACAAGGCCTTTCCAGTTAGTGACTGGTCGCGTCTGGAAAGGAACAGCTTTTGGTGGGTTCAAGAGTCGTTCCCAAGTCCCTTGGCTCGTTGAAAAGTACATGAAGAAGGAAATCAAAGTTGACGAATACATAACGCACAACCTGACGCTGGAAGAGATCAACAAAGCCTTTGATCTGATGCATGGAGGCGATTGCCTGCGCTGCGTTCTTTCGGCCCATCCCTAA

Protein sequence

MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATGVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGANEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKAFDLMHGGDCLRCVLSAHP
Homology
BLAST of Carg11533 vs. NCBI nr
Match: XP_022954014.1 (alcohol dehydrogenase class-3 [Cucurbita moschata] >XP_022954015.1 alcohol dehydrogenase class-3 [Cucurbita moschata] >KAG7014591.1 Alcohol dehydrogenase class-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785.4 bits (2027), Expect = 2.2e-223
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. NCBI nr
Match: XP_023548854.1 (alcohol dehydrogenase class-3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 783.9 bits (2023), Expect = 6.4e-223
Identity = 377/379 (99.47%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFS+NGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. NCBI nr
Match: KAG6576074.1 (Alcohol dehydrogenase class-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.5 bits (2022), Expect = 8.3e-223
Identity = 378/379 (99.74%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTS FSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSAFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. NCBI nr
Match: XP_038876338.1 (alcohol dehydrogenase class-3 [Benincasa hispida])

HSP 1 Score: 782.7 bits (2020), Expect = 1.4e-222
Identity = 375/379 (98.94%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFE+AKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFEIAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVI+DLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIIDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEY+THNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYVTHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. NCBI nr
Match: XP_022991611.1 (alcohol dehydrogenase class-3 [Cucurbita maxima] >XP_022991612.1 alcohol dehydrogenase class-3 [Cucurbita maxima])

HSP 1 Score: 781.2 bits (2016), Expect = 4.1e-222
Identity = 377/379 (99.47%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKID TAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDATAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. ExPASy Swiss-Prot
Match: Q96533 (Alcohol dehydrogenase class-3 OS=Arabidopsis thaliana OX=3702 GN=ADH2 PE=1 SV=2)

HSP 1 Score: 737.6 bits (1903), Expect = 6.8e-212
Identity = 352/375 (93.87%), Postives = 366/375 (97.60%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVA+EPNKPLVIEDVQVAPPQAGEVR+KILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAECRECKFCKSGKTNLCGKVR+AT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVG+MM+DR+SRFS+NGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAK AGASRIIGIDIDSKK+E AKKFG 
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGV 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPK+HDKPIQ+VIVDLTDGGVDYSFECIGNV+VMR+ALECCHKGWG SVIVGVAAS
Sbjct: 241 NEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITHNLTL EINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINK 360

Query: 361 AFDLMHGGDCLRCVL 376
           AFDL+H G CLRCVL
Sbjct: 361 AFDLLHEGTCLRCVL 375

BLAST of Carg11533 vs. ExPASy Swiss-Prot
Match: A2XAZ3 (Alcohol dehydrogenase class-3 OS=Oryza sativa subsp. indica OX=39946 GN=ADHIII PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 8.1e-205
Identity = 341/375 (90.93%), Postives = 359/375 (95.73%), Query Frame = 0

Query: 2   ATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEG 61
           +TQGQVITCKAAVAWE NKP+ IEDVQVAPPQAGEVRVKIL+TALCHTD YTWSGKDPEG
Sbjct: 4   STQGQVITCKAAVAWEANKPMTIEDVQVAPPQAGEVRVKILFTALCHTDHYTWSGKDPEG 63

Query: 62  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 121
           LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG
Sbjct: 64  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 123

Query: 122 VGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPT 181
           VGVMM+DR+SRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKI+P APLDKVCLLGCGV T
Sbjct: 124 VGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKINPQAPLDKVCLLGCGVST 183

Query: 182 GLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGAN 241
           GLGAVWNTAKVE GS VAIFGLGTVGLAVAEGAK+AGASRIIGIDIDSKKF++AK FG  
Sbjct: 184 GLGAVWNTAKVEAGSIVAIFGLGTVGLAVAEGAKSAGASRIIGIDIDSKKFDVAKNFGVT 243

Query: 242 EFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASG 301
           EFVNPK+HDKPIQQVIVDLTDGGVDYSFECIGNV+VMRSALECCHKGWG SVIVGVAASG
Sbjct: 244 EFVNPKDHDKPIQQVIVDLTDGGVDYSFECIGNVSVMRSALECCHKGWGTSVIVGVAASG 303

Query: 302 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKA 361
           QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+ KEIKVDEY+TH++ L +INKA
Sbjct: 304 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLNKEIKVDEYVTHSMNLTDINKA 363

Query: 362 FDLMHGGDCLRCVLS 377
           FDL+H G CLRCVL+
Sbjct: 364 FDLLHEGGCLRCVLA 378

BLAST of Carg11533 vs. ExPASy Swiss-Prot
Match: Q0DWH1 (Alcohol dehydrogenase class-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0815500 PE=2 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 1.8e-204
Identity = 340/375 (90.67%), Postives = 359/375 (95.73%), Query Frame = 0

Query: 2   ATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEG 61
           +TQGQVITCKAAVAWE N+P+ IEDVQVAPPQAGEVRVKIL+TALCHTD YTWSGKDPEG
Sbjct: 4   STQGQVITCKAAVAWEANRPMTIEDVQVAPPQAGEVRVKILFTALCHTDHYTWSGKDPEG 63

Query: 62  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 121
           LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG
Sbjct: 64  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 123

Query: 122 VGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPT 181
           VGVMM+DR+SRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKI+P APLDKVCLLGCGV T
Sbjct: 124 VGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKINPQAPLDKVCLLGCGVST 183

Query: 182 GLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGAN 241
           GLGAVWNTAKVE GS VAIFGLGTVGLAVAEGAK+AGASRIIGIDIDSKKF++AK FG  
Sbjct: 184 GLGAVWNTAKVEAGSIVAIFGLGTVGLAVAEGAKSAGASRIIGIDIDSKKFDVAKNFGVT 243

Query: 242 EFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASG 301
           EFVNPK+HDKPIQQVIVDLTDGGVDYSFECIGNV+VMRSALECCHKGWG SVIVGVAASG
Sbjct: 244 EFVNPKDHDKPIQQVIVDLTDGGVDYSFECIGNVSVMRSALECCHKGWGTSVIVGVAASG 303

Query: 302 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKA 361
           QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+ KEIKVDEY+TH++ L +INKA
Sbjct: 304 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLNKEIKVDEYVTHSMNLTDINKA 363

Query: 362 FDLMHGGDCLRCVLS 377
           FDL+H G CLRCVL+
Sbjct: 364 FDLLHEGGCLRCVLA 378

BLAST of Carg11533 vs. ExPASy Swiss-Prot
Match: P80572 (Alcohol dehydrogenase class-3 OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 8.4e-202
Identity = 335/377 (88.86%), Postives = 356/377 (94.43%), Query Frame = 0

Query: 2   ATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEG 61
           ATQGQVITCKAAVAWEPNKPL IEDV+VAPPQA EVR++IL+TALCHTDAYT  GKDPEG
Sbjct: 1   ATQGQVITCKAAVAWEPNKPLTIEDVEVAPPQANEVRIQILFTALCHTDAYTLGGKDPEG 60

Query: 62  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 121
           LFPCILGHEAAGIVESVGEGVT+V+PGDHVIP YQAEC ECKFCKS KTNLCGKVRAATG
Sbjct: 61  LFPCILGHEAAGIVESVGEGVTDVKPGDHVIPSYQAECGECKFCKSPKTNLCGKVRAATG 120

Query: 122 VGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPT 181
           VGVMM+DR+SRFS+ GKPIYHFMGTSTFSQYTVVHDVSVAKI P APLDKVCLLGCGVPT
Sbjct: 121 VGVMMADRKSRFSVKGKPIYHFMGTSTFSQYTVVHDVSVAKIHPDAPLDKVCLLGCGVPT 180

Query: 182 GLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGAN 241
           GLGAVWNTAKVEPGS VAIFGLGTVGLAVAEGAK+AGASRIIGIDIDS K++ AK FG  
Sbjct: 181 GLGAVWNTAKVEPGSIVAIFGLGTVGLAVAEGAKSAGASRIIGIDIDSNKYDTAKNFGVT 240

Query: 242 EFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASG 301
           EF+NPK+H+KPIQQVI+DLTDGGVDYSFEC+GNV+VMRSALECCHKGWG SVIVGVAASG
Sbjct: 241 EFINPKDHEKPIQQVIIDLTDGGVDYSFECLGNVSVMRSALECCHKGWGTSVIVGVAASG 300

Query: 302 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKA 361
           QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTL EINKA
Sbjct: 301 QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLLEINKA 360

Query: 362 FDLMHGGDCLRCVLSAH 379
           FDL+H G CLRCVL+ H
Sbjct: 361 FDLLHEGQCLRCVLAVH 377

BLAST of Carg11533 vs. ExPASy Swiss-Prot
Match: P93629 (Alcohol dehydrogenase class-3 OS=Zea mays OX=4577 GN=FDH PE=2 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 4.2e-201
Identity = 333/374 (89.04%), Postives = 357/374 (95.45%), Query Frame = 0

Query: 3   TQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEGL 62
           TQGQVITCKAAVA+EPNKPLVIEDVQVAPPQAGEVRVKIL+TALCHTD YTWSGKDPEGL
Sbjct: 5   TQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRVKILFTALCHTDHYTWSGKDPEGL 64

Query: 63  FPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATGV 122
           FPCILGHEAAGIVESVGEGVT+VQPGDHVIPCYQAEC+ECKFCKSGKTNLCGKVR+ATGV
Sbjct: 65  FPCILGHEAAGIVESVGEGVTDVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSATGV 124

Query: 123 GVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPTG 182
           GVMM+D +SRFS+NGKPIYHFMGTSTFSQYTVVHDVSVAKI+P APLDKVCLLGCGVPTG
Sbjct: 125 GVMMNDMKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKINPQAPLDKVCLLGCGVPTG 184

Query: 183 LGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGANE 242
           LGAVWNTAKVE GS VA+FGLGTVGLAVAEGAKAAGASR+IGIDID+KKF++AK FG  E
Sbjct: 185 LGAVWNTAKVESGSVVAVFGLGTVGLAVAEGAKAAGASRVIGIDIDNKKFDVAKNFGVTE 244

Query: 243 FVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASGQ 302
           FVNPKEHDKPIQQV+VDLTDGGVDYSFECIGNV++MR+ALEC  KGWG SVIVGVAASGQ
Sbjct: 245 FVNPKEHDKPIQQVLVDLTDGGVDYSFECIGNVSIMRAALECSDKGWGTSVIVGVAASGQ 304

Query: 303 EISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKAF 362
           EISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLV+KYMKKEIKVDEYITHN+ L +IN AF
Sbjct: 305 EISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVDKYMKKEIKVDEYITHNMNLADINDAF 364

Query: 363 DLMHGGDCLRCVLS 377
            L+H G CLRCVL+
Sbjct: 365 HLLHEGGCLRCVLA 378

BLAST of Carg11533 vs. ExPASy TrEMBL
Match: A0A6J1GRL8 (S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111456401 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.1e-223
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. ExPASy TrEMBL
Match: A0A6J1JR82 (S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111488173 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.0e-222
Identity = 377/379 (99.47%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKID TAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDATAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. ExPASy TrEMBL
Match: A0A0A0KBZ1 (S-(hydroxymethyl)glutathione dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_6G087920 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.7e-221
Identity = 373/379 (98.42%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVT+VQPGDHVIPCYQAECRECKFCKSGKTNLCGKVR AT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTDVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDR+SRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDID+KKFE+AKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDNKKFEIAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. ExPASy TrEMBL
Match: A0A1S3CB00 (S-(hydroxymethyl)glutathione dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498931 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.2e-221
Identity = 373/379 (98.42%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVT+VQPGDHVIPCYQAECRECKFCKSGKTNLCGKVR AT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTDVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRGAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDR+SRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFE+AK+FGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFEIAKQFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITHNLTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHNLTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. ExPASy TrEMBL
Match: A0A6J1HA66 (S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111460997 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 3.8e-221
Identity = 374/379 (98.68%), Postives = 376/379 (99.21%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDP APLDKVCLLGCGVP
Sbjct: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPAAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFE+AKKFGA
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFEIAKKFGA 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
            EFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS
Sbjct: 241 TEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKY+KKEIKVDEYITH LTLEEINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYLKKEIKVDEYITHELTLEEINK 360

Query: 361 AFDLMHGGDCLRCVLSAHP 380
           AFDLMHGGDCLRCVLSAHP
Sbjct: 361 AFDLMHGGDCLRCVLSAHP 379

BLAST of Carg11533 vs. TAIR 10
Match: AT5G43940.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 737.6 bits (1903), Expect = 4.9e-213
Identity = 352/375 (93.87%), Postives = 366/375 (97.60%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           MATQGQVITCKAAVA+EPNKPLVIEDVQVAPPQAGEVR+KILYTALCHTDAYTWSGKDPE
Sbjct: 1   MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPE 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
           GLFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAECRECKFCKSGKTNLCGKVR+AT
Sbjct: 61  GLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSAT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
           GVG+MM+DR+SRFS+NGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP
Sbjct: 121 GVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAK AGASRIIGIDIDSKK+E AKKFG 
Sbjct: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGV 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
           NEFVNPK+HDKPIQ+VIVDLTDGGVDYSFECIGNV+VMR+ALECCHKGWG SVIVGVAAS
Sbjct: 241 NEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
           GQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITHNLTL EINK
Sbjct: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINK 360

Query: 361 AFDLMHGGDCLRCVL 376
           AFDL+H G CLRCVL
Sbjct: 361 AFDLLHEGTCLRCVL 375

BLAST of Carg11533 vs. TAIR 10
Match: AT5G43940.2 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 727.6 bits (1877), Expect = 5.0e-210
Identity = 351/387 (90.70%), Postives = 366/387 (94.57%), Query Frame = 0

Query: 1   MATQGQVITCK------------AAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCH 60
           MATQGQVITCK            +AVA+EPNKPLVIEDVQVAPPQAGEVR+KILYTALCH
Sbjct: 1   MATQGQVITCKGTFILDPRTLTISAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCH 60

Query: 61  TDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSG 120
           TDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAECRECKFCKSG
Sbjct: 61  TDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSG 120

Query: 121 KTNLCGKVRAATGVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAP 180
           KTNLCGKVR+ATGVG+MM+DR+SRFS+NGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAP
Sbjct: 121 KTNLCGKVRSATGVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAP 180

Query: 181 LDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDID 240
           LDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAK AGASRIIGIDID
Sbjct: 181 LDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDID 240

Query: 241 SKKFELAKKFGANEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKG 300
           SKK+E AKKFG NEFVNPK+HDKPIQ+VIVDLTDGGVDYSFECIGNV+VMR+ALECCHKG
Sbjct: 241 SKKYETAKKFGVNEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKG 300

Query: 301 WGQSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEY 360
           WG SVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEY
Sbjct: 301 WGTSVIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEY 360

Query: 361 ITHNLTLEEINKAFDLMHGGDCLRCVL 376
           ITHNLTL EINKAFDL+H G CLRCVL
Sbjct: 361 ITHNLTLGEINKAFDLLHEGTCLRCVL 387

BLAST of Carg11533 vs. TAIR 10
Match: AT1G77120.1 (alcohol dehydrogenase 1 )

HSP 1 Score: 481.9 bits (1239), Expect = 4.8e-136
Identity = 224/376 (59.57%), Postives = 281/376 (74.73%), Query Frame = 0

Query: 1   MATQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPE 60
           M+T GQ+I CKAAVAWE  KPLVIE+V+VAPPQ  EVR+KIL+T+LCHTD Y W  K   
Sbjct: 1   MSTTGQIIRCKAAVAWEAGKPLVIEEVEVAPPQKHEVRIKILFTSLCHTDVYFWEAKGQT 60

Query: 61  GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAAT 120
            LFP I GHEA GIVESVGEGVT++QPGDHV+P +  EC EC+ C S ++N+C  +R  T
Sbjct: 61  PLFPRIFGHEAGGIVESVGEGVTDLQPGDHVLPIFTGECGECRHCHSEESNMCDLLRINT 120

Query: 121 GVGVMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180
             G M+ D +SRFSINGKPIYHF+GTSTFS+YTVVH   VAKI+P APLDKVC++ CG+ 
Sbjct: 121 ERGGMIHDGESRFSINGKPIYHFLGTSTFSEYTVVHSGQVAKINPDAPLDKVCIVSCGLS 180

Query: 181 TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGA 240
           TGLGA  N AK + G +VAIFGLG VGL  AEGA+ AGASRIIG+D +SK+F+ AK+FG 
Sbjct: 181 TGLGATLNVAKPKKGQSVAIFGLGAVGLGAAEGARIAGASRIIGVDFNSKRFDQAKEFGV 240

Query: 241 NEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAAS 300
            E VNPK+HDKPIQQVI ++TDGGVD S EC G+V  M  A EC H GWG +V+VGV + 
Sbjct: 241 TECVNPKDHDKPIQQVIAEMTDGGVDRSVECTGSVQAMIQAFECVHDGWGVAVLVGVPSK 300

Query: 301 GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINK 360
                T P   +  R  KGT FG +K ++ +P +VEKYM KE++++++ITH +   EINK
Sbjct: 301 DDAFKTHPMNFLNERTLKGTFFGNYKPKTDIPGVVEKYMNKELELEKFITHTVPFSEINK 360

Query: 361 AFDLMHGGDCLRCVLS 377
           AFD M  G+ +RC+++
Sbjct: 361 AFDYMLKGESIRCIIT 376

BLAST of Carg11533 vs. TAIR 10
Match: AT5G24760.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.0e-117
Identity = 198/372 (53.23%), Postives = 265/372 (71.24%), Query Frame = 0

Query: 4   QGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSGKDPEGLF 63
           Q QVITC AAVAW   +PLV+E+V+V+PPQ  E+R+K++ T+LC +D   W   + + L 
Sbjct: 10  QPQVITCNAAVAWRAGEPLVMEEVEVSPPQPLEIRIKVVCTSLCRSDLSAW---ESQSLL 69

Query: 64  PCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATGVG 123
           P I GHEAAGIVES+GEGVTE + GDHV+  +  EC  C+ C SGK+N+C +V      G
Sbjct: 70  PRIFGHEAAGIVESIGEGVTEFEKGDHVLAVFTGECGSCRHCISGKSNMC-QVLGMERKG 129

Query: 124 VMMSDRQSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPTGL 183
           +M SD+++RFSI GKP+YH+   S+FS+YTVVH     K+DP APL K+CLL CGV  GL
Sbjct: 130 LMHSDQKTRFSIKGKPVYHYCAVSSFSEYTVVHSGCAVKVDPLAPLHKICLLSCGVAAGL 189

Query: 184 GAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKFELAKKFGANEF 243
           GA WN A V+ GS+V IFGLGTVGL+VA+GAK  GA++I+G+DI+  K E AK FG  +F
Sbjct: 190 GAAWNVADVQKGSSVVIFGLGTVGLSVAQGAKLRGAAQILGVDINPAKAEQAKTFGVTDF 249

Query: 244 VNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQSVIVGVAASGQE 303
           +N  +  +PI QVI  +T GG D+SFEC+G+  +  +AL+ C  GWG +V +GV  +  E
Sbjct: 250 INSNDLSEPIPQVIKRMTGGGADFSFECVGDTGIATTALQSCSDGWGMTVTLGVPKAKPE 309

Query: 304 ISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHNLTLEEINKAFD 363
           +S      ++G+  KGT FGG+K +S +P L++KYM KEI +DE+ITHNL+ +EINKAF 
Sbjct: 310 VSAHYGLFLSGKSLKGTLFGGWKPKSDLPSLIDKYMNKEIMIDEFITHNLSFDEINKAFV 369

Query: 364 LMHGGDCLRCVL 376
           LM  G CLRCVL
Sbjct: 370 LMREGKCLRCVL 377

BLAST of Carg11533 vs. TAIR 10
Match: AT1G32780.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.5e-113
Identity = 198/383 (51.70%), Postives = 263/383 (68.67%), Query Frame = 0

Query: 3   TQGQVITCKAAVAWEPNKPLVIEDVQVAPPQAGEVRVKILYTALCHTDAYTWSG-KDPEG 62
           TQG+VITCKAAV W P  PLVI+++ V PPQ  EVRVKILY+++CHTD   W+G  + E 
Sbjct: 4   TQGKVITCKAAVVWGPKVPLVIQEICVDPPQKMEVRVKILYSSICHTDLGCWNGTNEAER 63

Query: 63  LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRAATG 122
            FP ILGHEA GIVESVGEGV +V+ GD+VIP +  EC ECK CK  ++NLC +      
Sbjct: 64  AFPRILGHEAVGIVESVGEGVKDVKEGDYVIPTFNGECGECKVCKREESNLCERYHVDPM 123

Query: 123 VGVMMSDRQSRFSI---------NGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKV 182
             VM++D  +RFS            +PIYHF+ TSTF++YTV+    V KIDP +PL ++
Sbjct: 124 KRVMVNDGGTRFSTTINKDGGSSQSQPIYHFLNTSTFTEYTVLDSACVVKIDPNSPLKQM 183

Query: 183 CLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKF 242
            LL CGV TG+GA WN A V+ G + A+FGLG+VGLAVAEGA+A GASRIIG+D ++ KF
Sbjct: 184 SLLSCGVSTGVGAAWNIANVKEGKSTAVFGLGSVGLAVAEGARARGASRIIGVDANASKF 243

Query: 243 ELAKKFGANEFVNPKEHDKPIQQVIVDLTDGGVDYSFECIGNVNVMRSALECCHKGWGQS 302
           E  K  G  +F+NPK+  KP+ Q+I ++T GGVDYSFEC GNV+V+R A    H GWG +
Sbjct: 244 EKGKLMGVTDFINPKDLTKPVHQMIREITGGGVDYSFECTGNVDVLREAFLSTHVGWGST 303

Query: 303 VIVGVAASGQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHN 362
           V+VG+  + + +   P +L  GR   G+ FGGFK +SQ+P   ++ MK  +K++ +IT+ 
Sbjct: 304 VLVGIYPTPRTLPLHPMELFDGRRITGSVFGGFKPKSQLPNFAQQCMKGVVKLEPFITNE 363

Query: 363 LTLEEINKAFDLMHGGDCLRCVL 376
           L  E+IN AF L+  G  LRC+L
Sbjct: 364 LPFEKINDAFQLLRDGKSLRCIL 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022954014.12.2e-223100.00alcohol dehydrogenase class-3 [Cucurbita moschata] >XP_022954015.1 alcohol dehyd... [more]
XP_023548854.16.4e-22399.47alcohol dehydrogenase class-3 [Cucurbita pepo subsp. pepo][more]
KAG6576074.18.3e-22399.74Alcohol dehydrogenase class-3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038876338.11.4e-22298.94alcohol dehydrogenase class-3 [Benincasa hispida][more]
XP_022991611.14.1e-22299.47alcohol dehydrogenase class-3 [Cucurbita maxima] >XP_022991612.1 alcohol dehydro... [more]
Match NameE-valueIdentityDescription
Q965336.8e-21293.87Alcohol dehydrogenase class-3 OS=Arabidopsis thaliana OX=3702 GN=ADH2 PE=1 SV=2[more]
A2XAZ38.1e-20590.93Alcohol dehydrogenase class-3 OS=Oryza sativa subsp. indica OX=39946 GN=ADHIII P... [more]
Q0DWH11.8e-20490.67Alcohol dehydrogenase class-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0... [more]
P805728.4e-20288.86Alcohol dehydrogenase class-3 OS=Pisum sativum OX=3888 PE=1 SV=1[more]
P936294.2e-20189.04Alcohol dehydrogenase class-3 OS=Zea mays OX=4577 GN=FDH PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GRL81.1e-223100.00S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JR822.0e-22299.47S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0KBZ11.7e-22198.42S-(hydroxymethyl)glutathione dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_6G0... [more]
A0A1S3CB002.2e-22198.42S-(hydroxymethyl)glutathione dehydrogenase OS=Cucumis melo OX=3656 GN=LOC1034989... [more]
A0A6J1HA663.8e-22198.68S-(hydroxymethyl)glutathione dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G43940.14.9e-21393.87GroES-like zinc-binding dehydrogenase family protein [more]
AT5G43940.25.0e-21090.70GroES-like zinc-binding dehydrogenase family protein [more]
AT1G77120.14.8e-13659.57alcohol dehydrogenase 1 [more]
AT5G24760.11.0e-11753.23GroES-like zinc-binding dehydrogenase family protein [more]
AT1G32780.11.5e-11351.70GroES-like zinc-binding dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 182..320
e-value: 1.3E-169
score: 565.8
NoneNo IPR availableGENE3D3.90.180.10coord: 13..372
e-value: 1.3E-169
score: 565.8
NoneNo IPR availablePANTHERPTHR43880:SF47S-(HYDROXYMETHYL)GLUTATHIONE DEHYDROGENASEcoord: 3..377
NoneNo IPR availablePANTHERPTHR43880ALCOHOL DEHYDROGENASEcoord: 3..377
IPR013154Alcohol dehydrogenase, N-terminalPFAMPF08240ADH_Ncoord: 35..162
e-value: 4.5E-25
score: 87.7
IPR014183Alcohol dehydrogenase class IIITIGRFAMTIGR02818TIGR02818coord: 10..375
e-value: 3.4E-190
score: 629.7
IPR014183Alcohol dehydrogenase class IIICDDcd08300alcohol_DH_class_IIIcoord: 8..375
e-value: 0.0
score: 798.362
IPR013149Alcohol dehydrogenase, C-terminalPFAMPF00107ADH_zinc_Ncoord: 206..327
e-value: 5.4E-26
score: 91.1
IPR002328Alcohol dehydrogenase, zinc-type, conserved sitePROSITEPS00059ADH_ZINCcoord: 68..82
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 326..375
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 3..193
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 168..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11533-RACarg11533-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008219 cell death
biological_process GO:0006069 ethanol oxidation
biological_process GO:0046292 formaldehyde metabolic process
biological_process GO:0010286 heat acclimation
biological_process GO:0048316 seed development
molecular_function GO:0106322 S-(hydroxymethyl)glutathione dehydrogenase NAD activity
molecular_function GO:0106321 S-(hydroxymethyl)glutathione dehydrogenase NADP activity
molecular_function GO:0080007 S-nitrosoglutathione reductase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity